Processing method of ganoderma lucidum spore extract suspension with antitumor effect

Abstract

The application belongs to the technical field of polysaccharide medicine extraction, and discloses a processing method of Ganoderma cochlear spore extract suspension with anti-tumor effect, which comprises the following steps: adding Ganoderma cochlear spore extract solid into a mixed solution of Ganoderma cochlear spore oil and glycerin monostearate, and uniformly mixing; the solid-liquid weight ratio is 1:8-10, and the mass of glycerin monostearate is 1-1.5% of the mass of Ganoderma cochlear spore oil. The fat-soluble components and water-soluble components in Ganoderma cochlear spore powder are extracted respectively, and the Ganoderma cochlear spore powder full-component health food prepared through mixing and preparation makes full use of all components, and the tumor inhibition rate of the Ganoderma cochlear spore extract suspension is significantly better than that of Ganoderma cochlear spore powder extract suspension and Ganoderma cochlear spore oil.

Description

Technical Field

[0001] This invention belongs to the field of polysaccharide drug extraction technology, specifically relating to a processing method for a suspension of Ganoderma lucidum spore extract with anti-tumor effects. Background Technology

[0002] Ganoderma lucidum spore powder is made from Ganoderma lucidum, a fungus belonging to the Polyporaceae family. Ganoderma lucidum (Leyss. ex Fr.)Karst.), Ganoderma lucidum ( Ganoderma sinense Zhao, Xu et Zhang), Pine and Fir Ganoderma ( Ganoderma tsugae The dried, mature spores of Ganoderma lucidum (Reishi mushroom). It is mainly distributed in Jiangxi, Zhejiang, Fujian, Liaoning, Jilin, and Guizhou provinces of my country. Studies on the chemical composition of Ganoderma lucidum spore powder by scholars from various countries show that it is rich and diverse in chemical components, mainly including polysaccharides, triterpenes and sterols, terpenes, amino acids, and alkaloids. Among these, polysaccharides, triterpenes, and sterols are the components with higher content and are also the main components that exert its pharmacological effects. Pharmacological activity studies have reported that the main components of Ganoderma lucidum have pharmacological effects such as antioxidant, antitumor, hypoglycemic, hypolipidemic, anti-inflammatory, anti-aging, immunomodulatory, hepatoprotective, and neuroprotective effects.

[0003] In recent years, many scholars both domestically and internationally have conducted in-depth research on the active ingredients and pharmacological effects of Ganoderma lucidum spore powder. Health foods using Ganoderma lucidum spore powder as the main raw material, such as broken-cell wall Ganoderma lucidum spore powder and Ganoderma lucidum spore oil, have excellent health benefits and market value. Ganoderma lucidum spore powder is readily available and inexpensive, but research reports and utilization of it are relatively few, and the research on its effective components is not clear. Summary of the Invention

[0004] This invention involves deep processing of Ganoderma lucidum spore powder, extracting its fat-soluble and water-soluble components separately, and then mixing and preparing the extracts to create a health food containing all the components of Ganoderma lucidum spore powder.

[0005] The objective of this invention is achieved through the following means:

[0006] A method for processing a suspension of Ganoderma lucidum spore extract with anti-tumor effects includes the following steps: adding solid Ganoderma lucidum spore extract to a mixture of Ganoderma lucidum spore oil and glyceryl monostearate and mixing evenly, wherein the solid-liquid weight ratio is 1:8-10, and the mass of glyceryl monostearate is 1-1.5% of the mass of Ganoderma lucidum spore oil.

[0007] Preferably, the solid-liquid weight ratio is 1:9.

[0008] Preferably, the mass of the glyceryl monostearate is 1% of the mass of the Ganoderma lucidum spore oil.

[0009] The processing method of the Ganoderma lucidum spore extract suspension with the antitumor effect comprises the following steps:

[0010] 1) Ganoderma lucidum spore oil preparation: the Ganoderma lucidum spore powder is broken to a broken wall rate of more than 90%, granulated and extracted to obtain Ganoderma lucidum spore oil and Ganoderma lucidum spore particles;

[0011] 2) Ganoderma lucidum spore extract preparation: the Ganoderma lucidum spore particles after oil removal in step 1) are soaked in water at 100 DEG C for 10-12 hours, decocted with water for 2-3 times, the extraction liquid is filtered and combined after each time, concentrated, collected and dried to obtain Ganoderma lucidum spore extract dry cake, which is crushed to obtain Ganoderma lucidum spore extract;

[0012] 3) Ganoderma lucidum spore extract suspension preparation: Ganoderma lucidum spore oil and glyceryl monostearate are mixed and stirred at 65-75 DEG C, the mass of glyceryl monostearate is 1-1.5% of the mass of Ganoderma lucidum spore oil, after glyceryl monostearate is completely dissolved, Ganoderma lucidum spore extract is added according to a solid-liquid weight ratio of 1:8-10, and stirring is continued at 65-75 DEG C until the solution is uniform, and then the solution is uniformly ground and mixed by using a colloid mill to obtain the Ganoderma lucidum spore extract suspension.

[0013] Preferably, the breaking wall in step 1) is that the Ganoderma lucidum spore powder is added into a ball mill and broken for 30-50 minutes to achieve a broken wall rate of more than 90%.

[0014] Preferably, after the breaking wall in step 1) is finished, the granulator is used for granulation, the granules are uniformly laid on the drying tray for drying, the laying thickness is 2-4 cm, the drying temperature is 100-120 DEG C, and the time is 1-2 hours.

[0015] Preferably, the extraction in step 1) is that the Ganoderma lucidum spore particle after granulation is loaded into a CO2 supercritical extraction device, the extraction pressure is set to 25-35 Mpa, the temperature is set to 40-50 DEG C, and the extraction is performed for 3-4 hours to obtain Ganoderma lucidum spore oil and Ganoderma lucidum spore particles.

[0016] Preferably, the decoction in step 2) is performed for 2 times, 8-10 times the weight of water is used for decoction for 2-3 hours in the first time, and 6-8 times the weight of water is used for decoction for 1-2 hours in the second time.

[0017] Preferably, the colloid mill in step 3) is uniformly mixed for 3-8 minutes.

[0018] Preferably, the magnetic stirring in step 3) is continuously performed for 30-40 minutes at 65-75 DEG C until the solution is in a uniform state.

[0019] Compared with the prior art, the present application has the following beneficial effects:

[0020] 1. Both Ganoderma lucidum spore oil and Ganoderma lucidum spore extract have anti-tumor effects. The combined use of the two has a better tumor-suppressing effect. However, the two raw materials are liquid and solid, respectively. Direct mixing will result in stratification. This invention uses glyceryl monostearate as a suspending agent for the suspension to achieve better dispersibility and anti-settling ability. Moreover, the tumor inhibition rate of this suspension is higher than that of using it alone, with a 1+1>2 effect. At the same time, the tumor inhibition rate of the Ganoderma lucidum spore extract suspension is significantly better than that of the Ganoderma lucidum red spore extract suspension, and the cost is low.

[0021] 2. This invention extracts the fat-soluble and water-soluble components from Ganoderma lucidum spore powder separately, and the resulting product, after mixing and preparation, can effectively make full use of all components of Ganoderma lucidum spore powder. Attached Figure Description

[0022] Figure 1 The images show the appearance and microscopic particle size comparison of the suspensions obtained by using glyceryl monostearate and Span 80 as surfactants in Examples 1 and Comparative Examples 1-3.

[0023] Figure 2 Comparison of appearance and particle size under a microscope for suspensions with different contents of glyceryl monostearate. Detailed Implementation

[0024] The following provides a detailed description of specific embodiments of the present invention. It should be understood that the specific embodiments described herein are for illustrative and explanatory purposes only and are not intended to limit the scope of the invention.

[0025] In the specific embodiments of this invention, unless otherwise specified, the reagents and materials used are all conventional commercially available products in the art and can be obtained through conventional commercial channels. The Ganoderma lucidum spore powder used in the embodiments is the dried mature spore of the fungus Ganoderma lucidum (Leyss. ex Fr.) Karst. of the Polyporaceae family, purchased from Anhui Huangshan Yunle Ganoderma Co., Ltd. It is a uniform brown powder in appearance, dry without mold, lumps, or impurities, and has the characteristic odor of Ganoderma lucidum spores. The polysaccharide content is ≥1.0g / 100g, the moisture content is ≤10g / 100g, the ash content is ≤3.0g / 100g, the lead (as Pb) content is ≤1.5mg / kg, the total arsenic (as As) content is ≤1.0mg / kg, and the mercury (as Hg) content is ≤0.3mg / kg. Example 1

[0026] Preparation of Ganoderma lucidum spore oil: The raw material of Ganoderma lucidum spore powder was added to a Belyi pulverizer for wall breaking, and wall breaking was performed for 30 minutes with a wall breaking rate of 95%. After wall breaking, a granulator was used for granulation. The granules were uniformly spread on a baking tray at a suitable thickness, dried, the spreading thickness was 2-4 cm, the drying temperature was 110°C, and the time was 1.5 hours. After granulation, the Ganoderma lucidum spore powder granules were loaded into a CO2 supercritical extraction device, the extraction pressure was set to 30 Mpa, the temperature was about 45°C, and the extraction time was about 4 hours, to obtain Ganoderma lucidum spore oil and oil-removed Ganoderma lucidum spore granules.

[0027] Preparation of Ganoderma lucidum spore extract: The oil-removed Ganoderma lucidum spore granules were soaked in an extraction tank at 100°C for 12 hours, and were decocted twice with water, 10 times the weight of water for the first time for 3 hours and 8 times the weight of water for the second time for 2 hours. The two extraction liquids were filtered through a plate and frame filter, temporarily stored in an intermediate storage tank, concentrated to an appropriate density through a double-effect concentrator under reduced pressure, and the extract was collected. The extract was dried in vacuum to obtain a Ganoderma lucidum spore extract dry cake, which was further pulverized to obtain a Ganoderma lucidum spore extract fine powder.

[0028] Preparation of Ganoderma lucidum spore oil spore extract suspension: The water temperature of a mixing tank was set to 70°C, Ganoderma lucidum spore oil and glycerol monostearate were added to the mixing tank and stirred, the mass of glycerol monostearate was 1% of the mass of Ganoderma lucidum spore oil, and after the glycerol monostearate was completely dissolved, Ganoderma lucidum spore powder extract was added according to a solid-liquid weight ratio of 1:9, and the mixture was continuously stirred for 30 minutes to fully mix, until the solution was uniform. After mixing, the bottom discharge valve was opened, the oil liquid was put into the material bin of a colloid mill, and the mixture was uniformly mixed again for 5 minutes in the colloid mill, and then the mixture was loaded into an oil storage tank.

[0029] After detection, the polysaccharide content was 2.5 g / 100 g, the ergosterol content was 150.3 mg / 100 g, and the Ganoderma lucidum triterpene content was 20.2 g / 100 g. Example 2

[0030] Preparation of Ganoderma lucidum spore oil: The raw material of Ganoderma lucidum spore powder was added to a Belyi pulverizer for wall breaking, and wall breaking was performed for 30 minutes with a wall breaking rate of 95%. After wall breaking, a granulator was used for granulation. The granules were uniformly spread on a baking tray at a suitable thickness, dried, the spreading thickness was 2-4 cm, the drying temperature was 110°C, and the time was 1.5 hours. After granulation, the Ganoderma lucidum spore powder granules were loaded into a CO2 supercritical extraction device, the extraction pressure was set to 30 Mpa, the temperature was about 45°C, and the extraction time was about 4 hours, to obtain Ganoderma lucidum spore oil and oil-removed Ganoderma lucidum spore granules.

[0031] Preparation of Ganoderma lucidum spore extract: After the oil was removed, the Ganoderma lucidum spore particles were soaked in the extraction tank at 100°C for 12 hours, and then boiled with water twice, 10 times the weight of water for the first time for 3 hours, and 8 times the weight of water for the second time for 2 hours. The two extraction solutions were filtered through a plate and frame filter, temporarily stored in an intermediate storage tank, concentrated to an appropriate density by a double-effect concentrator under reduced pressure, and then collected. The paste was dried in a vacuum to obtain a Ganoderma lucidum spore extract dry paste block, which was then crushed to obtain a Ganoderma lucidum spore extract fine powder extract.

[0032] Preparation of Ganoderma lucidum spore oil spore extract suspension: The water temperature of the mixing tank was set to 70°C, and Ganoderma lucidum spore oil and glyceryl monostearate were added to the mixing tank and stirred. The mass of glyceryl monostearate was 1% of the mass of Ganoderma lucidum spore oil. After the glyceryl monostearate was completely dissolved, the Ganoderma lucidum spore powder extract was added according to a solid-liquid weight ratio of 1:8, and the solution was continuously stirred for 25 minutes until it was uniform. After the mixing was completed, the bottom discharge valve was opened, the oil was placed in the colloid mill hopper, and the mixture was uniformly mixed again for 5 minutes. The mixture was then loaded into the oil storage tank. Example 3

[0033] Preparation of Ganoderma lucidum spore oil: The Ganoderma lucidum spore powder was broken down in a Belyi crusher for 30 minutes, with a breakage rate of more than 90%. After the breakage, the particles were granulated using a granulator. The granules were evenly spread on the baking tray at an appropriate thickness, dried, and then collected. The thickness of the spread was 2-4 cm, the drying temperature was 120°C, and the drying time was 1 hour. After granulation, the Ganoderma lucidum spore powder particles were loaded into a CO2 supercritical extraction device, set to an extraction pressure of 30 Mpa and a temperature of about 45°C, and extracted for about 4 hours to obtain Ganoderma lucidum spore oil and oil-removed Ganoderma lucidum spore particles.

[0034] Preparation of Ganoderma lucidum spore extract: After the oil was removed, the Ganoderma lucidum spore particles were soaked in the extraction tank at 100°C for about 12 hours, and then boiled with water twice, 10 times the weight of water for the first time for 3 hours, and 8 times the weight of water for the second time for 2 hours. The two extraction solutions were filtered through a plate and frame filter, temporarily stored in an intermediate storage tank, concentrated to an appropriate density by a double-effect concentrator under reduced pressure, and then collected. The paste was dried in a vacuum to obtain a Ganoderma lucidum spore extract dry paste block, which was then crushed to obtain a Ganoderma lucidum spore extract fine powder extract.

[0035] Preparation of Ganoderma lucidum spore oil spore extract suspension: The water temperature of the mixing tank was set to 70°C, and Ganoderma lucidum spore oil and glyceryl monostearate were added to the mixing tank and stirred. The mass of glyceryl monostearate was 1% of the mass of Ganoderma lucidum spore oil. After the glyceryl monostearate was completely dissolved, the Ganoderma lucidum spore powder extract was added according to a solid-liquid weight ratio of 1:8, and the solution was continuously stirred for 25 minutes until it was uniform. After the mixing was completed, the bottom discharge valve was opened, the oil was placed in the colloid mill hopper, and the mixture was uniformly mixed again for 5 minutes. The mixture was then loaded into the oil storage tank.

[0036] Comparative Example 1

[0037] The remaining steps are the same as Example 1, except that the mixture after adding the Ganoderma lucidum spore powder extract according to a solid-liquid weight ratio of 1:9 is subjected to high-speed shearing for 5 min (4000 rpm), and the shearing is cooled with an ice water bath.

[0038] Comparative Example 2

[0039] The remaining steps are the same as Example 1, except that the glyceryl monostearate is replaced by Span 80 at a corresponding dose.

[0040] Comparative Example 3

[0041] The remaining steps are the same as Example 1, except that the glyceryl monostearate is replaced by Span 80 at a corresponding dose, and the mixture after adding the Ganoderma lucidum spore powder extract according to a solid-liquid ratio of 1:9 is subjected to high-speed shearing for 5 min (4000 rpm), and the shearing is cooled with an ice water bath.

[0042] A small amount of each of Example 1 and Comparative Examples 1-3 is taken, and the change in powder particle size is observed under a microscope after being placed for different times. The properties of the samples after being placed for 24 h are also observed. The results are shown in Table 2. Figure 1 .

[0043] The microscopic images show that, whether dispersed by magnetic stirring or by using high-speed shearing, the dispersing effect of Span 80 is better than that of glyceryl monostearate. Comparing the microscopic images of magnetic stirring and high-speed shearing, the dispersing effect of high-speed shearing is not as good as that of magnetic stirring, and even the phenomenon of particle aggregation occurs. However, after the samples are placed for 24 h, obvious sedimentation occurs in the Span 80 group, while the particles in the glyceryl monostearate group are still relatively uniform, indicating that the suspending effect of glyceryl monostearate is better.

[0044] Test Example 1 Investigation of the Effect of the Amount of Surfactant on the Dispersion of Solid Powder

[0045] The maximum daily dosage of glyceryl monostearate for oral administration is 272 mg. The weight of glyceryl monostearate is set to be 0.5%, 1%, 1.5%, and 2% of the amount of Ganoderma lucidum spore oil, respectively. After being heated and melted, glyceryl monostearate is added to Ganoderma lucidum spore oil (prepared according to the method of Example 1), and then Ganoderma lucidum spore powder extract (prepared according to the method of Example 1) is added, to obtain four samples. The samples are subjected to magnetic stirring for 0.5 h, and a small amount of each sample is taken and observed for changes in powder particle size under a microscope. The properties of the samples after being placed for 24 h are also observed. The specific sample amounts are shown in Table 1.

[0046]

[0047] The appearance and particle size are investigated, and the results are shown in Table 2. Figure 2

[0048] 2) Sedimentation ratio and redispersion investigation

[0049] The suspension of Ganoderma lingzhi spore extract obtained after magnetic stirring was poured into a measuring cylinder, and the initial volume H0 was recorded. The sedimentation surface volume (H 3h , H ∝ ) was recorded at 3 h, and different time points until no change was observed. The sedimentation volume ratio F (F 3h , F ∝ ) was calculated, and the results are shown in Table 2.

[0050]

[0051] In summary, the above four groups of samples all formed relatively uniform brown suspensions without obvious powder particles at the bottom. With the increase of the amount of glyceryl monostearate, the particle size distribution was more uniform, and the powder particle distribution was more uniform. When the amount of glyceryl monostearate reached 1% of the mass of Ganoderma lingzhi spore oil, good anti-sedimentation ability could be achieved, and the effect gradually increased with the increase of the amount, but the effect was not obvious. At the same time, with the increase of the amount of glyceryl monostearate, the redispersibility decreased. The last group had abnormal data, which may be due to the fact that the group had too much viscosity, and the original conditions could not make it completely settle, resulting in a lower redispersion time. In summary, the most preferred amount of glyceryl monostearate is 1% of the mass of Ganoderma lingzhi spore oil.

[0052] Test Example 2

[0053] 1. Purpose of the experiment

[0054] To detect whether the 1-4 samples of the drug have growth inhibition effect on mouse sarcoma S180 cell transplanted tumors and the strength of the effect, and to provide experimental basis for clinical application.

[0055] 2. Experimental materials

[0056] 2.1 Test drug

[0057] 2.1.1 Sample No. 1

[0058] Name: Sample No. 1, Ganoderma lingzhi spore oil (prepared according to the method of Example 1)

[0059] Characteristics: yellow oily solution

[0060] Concentration: 800 mg / mL

[0061] Preparation method: take an appropriate amount of sample No. 1 working stock solution, then dilute it with soybean oil (manufacturer: Shandong Ruisheng Pharmaceutical Auxiliary Co., Ltd., batch number: DD20210701, specification: 500 mL) to 50 mg / mL, 25 mg / mL, 12.5 mg / mL working solution, the administration volume is 0.4 ml / 20 g, and the administration dose is 1000 mg / kg, 500 mg / kg, 250 mg / kg respectively.

[0062] 2.1.2 Sample No. 2

[0063] Name: Sample No. 2, Ganoderma lucidum spore extract (prepared according to the method of Example 1)

[0064] Characteristics: brown powder

[0065] Preparation method: accurately weigh 0.10 g of sample No. 2, add 0.5% CMC-Na 10 ml to prepare a 10.0 mg / mL solution; accurately weigh 0.05 g of sample No. 2, add 0.5% CMC-Na 10 ml to prepare a 5.0 mg / mL solution; accurately weigh 0.025 g of sample No. 2, add 0.5% CMC-Na 10 ml to prepare a 2.5 mg / mL solution; the concentration of the solution is 10.0 g / ml, 5.0 g / ml, 2.5 g / ml. The administration volume is 0.4 mL / 20 g, and the administration dose is 200 mg / kg, 100 mg / kg, 50 mg / kg.

[0066] 2.1.3 Sample No. 3

[0067] Name: Sample No. 3

[0068] Characteristics: Ganoderma lucidum spore oil spore extract suspension (prepared according to the method of Example 1)

[0069] Concentration: 800 mg / mL

[0070] Preparation method: take an appropriate amount of sample No. 3 working stock solution, then dilute it with soybean oil (manufacturer: Shandong Ruisheng Pharmaceutical Auxiliary Co., Ltd., batch number: DD20210701, specification: 500 mL) to 50 mg / mL, 25 mg / mL, 12.5 mg / mL working solution, the administration volume is 0.4 ml / 20 g, and the administration dose is 1000 mg / kg, 500 mg / kg, 250 mg / kg respectively.

[0071] 2.1.4 Sample No. 4

[0072] Name: Sample No. 4

[0073] Characteristics: Red mushroom spore oil spore extract suspension (preparation method same as example 1, the difference is that the raw material uses red mushroom)

[0074] Concentration: 800 mg / mL

[0075] Preparation method: Take an appropriate amount of sample No. 4 working mother liquor, then dilute it with soybean oil (manufacturer: Shandong Ruisheng Pharmaceutical Auxiliary Co., Ltd., batch number: DD20210701, specification: 500 mL) into working solutions of 50 mg / mL, 25 mg / mL, and 12.5 mg / mL, respectively. The administration volume is 0.4 ml / 20 g, and the administration dose is 1000 mg / kg, 500 mg / kg, and 250 mg / kg, respectively.

[0076] 2.1 Control drug

[0077] Name: 5-FU (5-fluorouracil)

[0078] Providing unit: Tianjin Jinyao Pharmaceutical Co., Ltd.

[0079] Preparation method: One bottle of injection is 0.25 g / 10 ml, take 1 ml and add 7.33 ml of physiological saline (manufacturer: Jiangsu Huaian Shuanghe Pharmaceutical Co., Ltd.; specification: 500 ml: 4.5 g) to shake up to 3 mg / ml solution, store at 4℃, the administration volume is 0.2 ml / 20 g, and the administration dose is 30 mg / kg.

[0080] 2.2 Experimental animals

[0081] Source, lineage, strain: ICR mice, provided by Hangzhou Medical College (production) (experimental animal production license: SCXK (Zhejiang) 2019-0002).

[0082] Weeks of age: 6-8 weeks of age

[0083] Gender: female

[0084] 2.3 The experimental animal grouping is shown in Table 3

[0085]

[0086] 2.4 Experimental method

[0087] ICR mice were inoculated with ascitic tumor (ascitic tumor was taken under sterile operation, diluted with physiological saline to 1:4 cell suspension, container was placed on ice block, and cell was mixed before each aspiration, 0.2 ml was inoculated to right forelimb axillary of each mouse), and weighed 24 hours after inoculation, and randomly divided into 14 groups. The first administration was performed 24 hours after inoculation (d1), and 1-4 samples were administered by gavage, once a day, for 9 times. The administration volume was 0.4 ml / 20 g. The tumor-bearing mice were sacrificed on the 10th day after inoculation (d10), weighed, and tumor mass was separated and weighed, and the obtained data was statistically processed (t-test).

[0088] 3. Results and discussion

[0089] The results of mouse sarcoma S180 cell transplantation tumor model experiment are shown in Table 4:

[0090] Sample 1 was administered by gavage at 1000 mg / kg, 500 mg / kg, and 250 mg / kg, once a day, for 10 days;

[0091] Sample 2 was administered by gavage at 200 mg / kg, 100 mg / kg, and 50 mg / kg, once a day, for 10 days;

[0092] Sample 3 was administered by gavage at 1000 mg / kg, 500 mg / kg, and 250 mg / kg, once a day, for 10 days;

[0093] Sample 4 was administered by gavage at 1000 mg / kg, 500 mg / kg, and 250 mg / kg, once a day, for 10 days;

[0094] Positive drug 5-FU was administered by tail vein injection at 30 mg / kg, once every 2 days, for 10 days.

[0095] During the experiment, no obvious effect of samples 1-4 at various doses on the body weight of experimental animals was found, but the positive drug 5-FU had a very significant inhibitory effect on the body weight of experimental animals.

[0096]

[0097] 4. Experimental conclusion

[0098] Both Ganoderma cochlear spore oil and Ganoderma cochlear spore extract have anti-tumor effect, but the inhibition rate of the mixture is higher than that of single use, and has the effect of 1+1>2. The inhibition rate of Ganoderma cochlear spore extract mixture is also significantly higher than that of Ganoderma lucidum spore powder extract mixture.

Claims

1. A method for processing Ganoderma amym spore extract suspension having an antitumor effect, characterized by The method comprises the following steps: adding Ganoderma lucidum spore extract solid into Ganoderma lucidum spore oil and glyceryl monostearate mixed solution and uniformly mixing, the solid-liquid weight ratio is 1:8-10, and the mass of glyceryl monostearate is 1-1.5% of the mass of Ganoderma lucidum spore oil; the preparation method of the Ganoderma lucidum spore extract comprises the following steps: breaking the wall of Ganoderma lucidum spore powder to more than 90% of the wall breaking rate, granulating and extracting to obtain Ganoderma lucidum spore oil and Ganoderma lucidum spore particles; immersing the Ganoderma lucidum spore particles in water at 100 DEG C for 10-12 hours, decocting twice or three times, filtering the extraction liquid respectively, combining, concentrating, collecting the extract, vacuum drying to obtain Ganoderma lucidum spore extract dry extract block, and crushing to obtain Ganoderma lucidum spore extract.

2. The method of claim 1, wherein The solid-liquid weight ratio is 1:

9.

3. The method of claim 1 wherein The mass of glyceryl monostearate is 1% of the mass of Ganoderma lucidum spore oil.

4. The method of claim 1 wherein The method specifically comprises the following steps: 1) Ganoderma lucidum spore oil preparation: breaking the wall of Ganoderma lucidum spore powder to more than 90% of the wall breaking rate, granulating and extracting to obtain Ganoderma lucidum spore oil and Ganoderma lucidum spore particles; 2) Ganoderma lucidum spore extract preparation: immersing the Ganoderma lucidum spore particles after removing fat in step 1) in water at 100 DEG C for 10-12 hours, decocting twice or three times, filtering the extraction liquid respectively, combining, concentrating, collecting the extract, vacuum drying to obtain Ganoderma lucidum spore extract dry extract block, and crushing to obtain Ganoderma lucidum spore extract; 3) Ganoderma lucidum spore extract suspension preparation: mixing Ganoderma lucidum spore oil and glyceryl monostearate at 65-75 DEG C, the mass of glyceryl monostearate is 1-1.5% of the mass of Ganoderma lucidum spore oil, after glyceryl monostearate is completely dissolved, adding Ganoderma lucidum spore extract according to the solid-liquid weight ratio of 1:8-10, continuing to stir at 65-75 DEG C until the solution is uniform, and then uniformly grinding and mixing by using a colloid mill.

5. The method of claim 4, wherein In step 1), the wall breaking is specifically adding Ganoderma lucidum spore powder into a ball mill and wall breaking for 30-50 minutes to achieve more than 90% of the wall breaking rate.

6. The method of claim 4, wherein After the wall breaking in step 1) is completed, the granulator is used for granulation, the particles are uniformly laid on the drying tray for drying, the laying thickness is 2-4 cm, the drying temperature is 100-120 DEG C, and the time is 1-2 hours.

7. The method of claim 4, wherein In step 1), the extraction is loading the granulated Ganoderma lucidum spore particle into a CO2 supercritical extraction device, setting the extraction pressure to 25-35 Mpa and the temperature to 40-50 DEG C, and extracting for 3-4 hours to obtain Ganoderma lucidum spore oil and Ganoderma lucidum spore particles.

8. The method of claim 4, wherein In step 2), the water decocting is performed twice, the first time is decocting for 2-3 hours with 8-10 times the weight of water, and the second time is decocting for 1-2 hours with 6-8 times the weight of water.

9. The method of claim 4, wherein In step 3), the colloid mill is uniformly mixed for 3-8 minutes.

10. The method of claim 4, wherein In step 3), the solution is continuously stirred at 65-75 DEG C for 30-40 minutes until the solution is in a uniform state.

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