Composite nanoemulsion as well as preparation method and application thereof

Abstract

The invention relates to a composite nanoemulsion as well as a preparation method and application thereof. Each liter of the composite nanoemulsion is prepared from 35g to 50g of fat-soluble vitamin, 85g to 100g of water-soluble vitamin, 2g to 5g of selenium homolanonine, 5g to 25g of yucca extract, 15g to 40g of nicotinamide, 40g to 60g of urea, 140g to 160g of emulsifier, 25g to 45g of cosolvent and 8g to 15g of stabilizer. According to the composite nanoemulsion, the fat-soluble vitamins, the water-soluble vitamins, the natural functional ingredient yucca extract and the organic selenium are synergistically carried together, and the composite nanoemulsion not only has more and higher nutritional ingredients, but also is good in stability, long in storage life, small in particle size and narrow in distribution.

Description

Technical Field

[0001] The present invention relates to the technical field of nanoemulsions, and in particular to a composite nanoemulsion and a preparation method and application thereof. Background Art

[0002] Vitamins are essential micronutrients for animal growth and development, immune regulation, and metabolic function. In livestock, poultry, pets, and aquaculture, vitamin supplementation plays a crucial role in improving production performance, enhancing disease resistance, and generally improving animal health. However, traditional veterinary vitamin formulations (such as tablets and capsules) suffer from low bioavailability and poor stability, limiting their effectiveness. In recent years, nanoemulsion technology has become a research hotspot for vitamin delivery systems due to its potential to enhance the solubility, absorption, and stability of fat-soluble vitamins.

[0003] At present, some veterinary vitamin nanoemulsion products have been launched, but the existing technology still has the following shortcomings: First, the vitamin composition is limited and insufficient. Currently, veterinary vitamin nanoemulsions on the market primarily contain fat-soluble vitamins (such as vitamins A, D, E, and K), while water-soluble vitamins (such as B vitamins and vitamin C) are rarely added, resulting in an unbalanced nutritional profile. Furthermore, some products have low vitamin content, failing to achieve the desired nutritional supplementation effect.

[0004] Second, there is a lack of natural functional ingredients. Yucca schidigera extract (rich in active ingredients such as saponins and polysaccharides) has multiple benefits in animal nutrition, including reducing ammonia emissions, improving intestinal health, enhancing immunity, and providing antioxidant benefits. However, existing veterinary vitamin nanoemulsions rarely incorporate these natural plant ingredients, failing to fully exploit their synergistic effects. This is particularly true in intensive farming environments, where they are unable to effectively alleviate oxidative stress and intestinal health issues in animals.

[0005] Third, the key trace element selenium is not fortified. Selenium is an essential trace element for animals, crucial for improving reproductive performance, enhancing immunity, and enhancing antioxidant capacity. However, existing nanoemulsion systems generally do not add selenium.

[0006] To address these shortcomings, the applicants discovered through experimental research that simply increasing the amount of water-soluble and fat-soluble vitamins significantly reduces system stability, making phase separation or precipitation more likely and difficult to form a stable, uniform nanoemulsion system. While inorganic selenium is highly water-soluble, it suffers from low bioavailability and potential toxicity, making it unsuitable for veterinary nanoemulsion formulations. Organic selenium, due to its complex molecular structure, exhibits poor solubility and dispersibility in nanoemulsion systems, presenting significant technical obstacles. Furthermore, the saponin component in yucca extract, due to its surface-active properties, may interfere with the integrity of the nanoemulsion's interfacial membrane structure, leading to stability issues such as droplet aggregation or system demulsification. Summary of the Invention

[0007] The purpose of the present invention is to provide a composite nanoemulsion and its preparation method and application. The composite nanoemulsion not only achieves the synergistic high-content encapsulation of water-soluble and fat-soluble vitamins, but also innovatively integrates active ingredients such as organic selenium and yucca extract, while maintaining the stability of the system, significantly improving the nutritional value and functional properties of the product.

[0008] In order to achieve the above object, the technical solution adopted by the present invention is as follows: The present invention provides a composite nanoemulsion, wherein each liter of the composite nanoemulsion comprises: 35g to 50g of fat-soluble vitamins, 85g to 100g of water-soluble vitamins, 2g to 5g of selenium homolanolin, 5g to 25g of yucca extract, 15g to 40g of nicotinamide, 40g to 60g of urea, 140g to 160g of emulsifier, 25g to 45g of solubility aid and 8g to 15g of stabilizer, wherein: the fat-soluble vitamins include vitamin A, vitamin D and vitamin E; the water-soluble vitamins include vitamin A, vitamin D and vitamin E; The soluble vitamins include vitamin B and vitamin C. The emulsifier is selected from at least two polyethylene glycol glycerol ricinoleates, one polyethylene glycol glycerol ricinoleate having a polyethylene glycol chain degree of polymerization of 31-40, and the other polyethylene glycol glycerol ricinoleate having a polyethylene glycol chain degree of polymerization of 20-30. The mass ratio of the polyethylene glycol glycerol ricinoleate having a polyethylene glycol chain degree of polymerization of 20-30 to the polyethylene glycol glycerol ricinoleate having a polyethylene glycol chain degree of polymerization of 31-40 is (3-12):1. Unless otherwise specified, the degree of polymerization (DP) in this invention refers to the number of repeating units in a polymer molecular chain and is an important parameter describing polymer chain length. For example, a polyethylene glycol chain degree of polymerization of 31-40 refers to 31-40 repeating -CH2-CH2-O- units.

[0009] This invention optimizes the performance of the nanoemulsion system through the synergistic action of urea and niacinamide. Specifically, the addition of urea effectively disrupts the hydrogen bond network between water molecules, significantly reducing the viscosity of the aqueous phase, thereby promoting the uniform dispersion of vitamin B2 and other components, inhibiting droplet aggregation, and maintaining the stability of the nanoemulsion. Simultaneously, the niacinamide molecules, through bridging, form stable molecular connections at the oil-water interface, ensuring the long-term dispersion and stability of vitamin B2 and other components in the aqueous phase.

[0010] In addition, the present invention significantly reduces the interfacial tension of the system by optimizing the composition of the emulsification system and the organic selenium component, further promotes the formation of nano-scale emulsion droplets, improves the particle size distribution and storage stability of the nanoemulsion, and also increases the solubility of the oil phase components in the aqueous phase.

[0011] In some embodiments, the content of vitamin A in the composite nanoemulsion is 4-7 g / L, that is, each liter of composite nanoemulsion contains 4-7 g of vitamin A. Preferably, the content of vitamin A in the composite nanoemulsion is 5-6.5 g / L.

[0012] In some embodiments, the content of vitamin D in the composite nanoemulsion is 0.1-0.5 g / L, more preferably 0.2-0.4 g / L.

[0013] In some embodiments, the content of vitamin E in the composite nanoemulsion is 30-40 g / L, more preferably 33-38 g / L.

[0014] In some embodiments, the content of vitamin B in the composite nanoemulsion is 2-4 g / L, more preferably 2-3 g / L.

[0015] In some embodiments, the content of vitamin C in the composite nanoemulsion is 80-100 g / L, more preferably 85-95 g / L.

[0016] In some preferred embodiments, the vitamin A is vitamin A palmitate.

[0017] In some preferred embodiments, the vitamin D is vitamin D3.

[0018] In some preferred embodiments, the vitamin E is vitamin E acetate.

[0019] In some preferred embodiments, the vitamin B is vitamin B2.

[0020] In some embodiments, the cosolvent is selected from one or more of polyethylene glycol and propylene glycol.

[0021] In some embodiments, the stabilizer is sodium metabisulfite, sodium bicarbonate, and ethylenediaminetetraacetate. Preferably, the content of sodium metabisulfite in the composite nanoemulsion is 0.5-2.5 g / L, more preferably 1-2 g / L. The content of sodium bicarbonate in the composite nanoemulsion is 8-12 g / L, more preferably 9-11 g / L. The content of ethylenediaminetetraacetate in the composite nanoemulsion is 0.1-0.5 g / L, more preferably 0.2-0.4 g / L.

[0022] In some specific embodiments, the EDTA salt is disodium EDTA.

[0023] In some embodiments, the average particle size of the composite nanoemulsion is no more than 50 nm. Further, the average particle size of the composite nanoemulsion is 40 nm to 50 nm.

[0024] In some specific embodiments, each liter of the composite nanoemulsion comprises: 4g~7g vitamin A, 0.1g~0.5g vitamin D, 30g~40g vitamin E, 2g~4g vitamin B, 80g~100g vitamin C, 2g~5g homolanine selenium, 10g~20g yucca extract, 15g~25g niacinamide, 45g~55g urea, 110g~120g polyethylene glycol chain polymerization degree of 20~30 polyethylene glycol glycerol ricinoleate, 10g~20g polyethylene glycol chain polymerization degree of 31~40 polyethylene glycol glycerol ricinoleate, 25g~45g solubility aid, 8g~12g sodium bicarbonate, 0.5g~2.5g sodium metabisulfite and 0.1g~0.5g ethylenediaminetetraacetate.

[0025] In some embodiments, the composite nanoemulsion further comprises 10 g to 20 g of xylooligosaccharide.

[0026] In some embodiments, the dispersion medium of the composite nanoemulsion is water.

[0027] A second aspect of the present invention is to provide a method for preparing the composite nanoemulsion as described above, comprising the steps of: (1) After mixing the fat-soluble vitamins, polyethylene glycol glycerol ricinoleate with a polyethylene glycol chain polymerization degree of 20 to 30 and a portion of the dispersion medium are added and mixed uniformly to prepare a first mixed solution; (2) mixing the yucca extract with polyethylene glycol glycerol ricinoleate having a polyethylene glycol chain polymerization degree of 31 to 40, adding a cosolvent and a portion of a dispersion medium, and mixing them uniformly to prepare a second mixed solution; (3) dissolving urea in the remaining dispersion medium, adding a stabilizer, water-soluble vitamins, niacinamide, and selenium homolanolinate, and mixing them evenly to prepare a third mixed solution; (4) Evenly mixing the first mixed solution, the second mixed solution, and the third mixed solution to prepare the composite nanoemulsion.

[0028] In some embodiments, the step (1) mixes the fat-soluble vitamins at 50-60°C.

[0029] In some embodiments, the mixing in steps (1) to (4) is performed under stirring, and the stirring speed is independently 300-500 r / min.

[0030] The third aspect of the present invention is to provide an application of the composite nanoemulsion as described above, wherein the application comprises adding the composite nanoemulsion as a feed additive to feed or drinking water.

[0031] Due to the application of the above technical solution, the present invention has the following advantages compared with the prior art: The composite nanoemulsion of the present invention realizes the synergistic co-loading of fat-soluble vitamins, water-soluble vitamins, the natural functional ingredient yucca extract and organic selenium. The composite nanoemulsion not only has more and higher nutritional components, but also has good stability, long storage period, small particle size and narrow distribution. DETAILED DESCRIPTION

[0032] The present invention is further described below with reference to the following examples. However, the present invention is not limited to the following examples. The implementation conditions used in the examples may be further adjusted according to the specific requirements of the application. Unspecified implementation conditions are conventional conditions in the industry. The technical features involved in the various embodiments of the present invention may be combined with each other as long as they do not conflict with each other.

[0033] Unless otherwise specified, the raw materials involved in the following examples and comparative examples are all commercially available products.

[0034] Unless otherwise specified, "parts" hereinbelow are parts by mass.

[0035] Example 1: This example provides a composite nanoemulsion, the components of which are as follows: Vitamin E acetate (purchased from DSM Vitamins (Shanghai) Co., Ltd., production batch number 0420085) 35 parts, vitamin A palmitate (purchased from Shangyu Xinhecheng Biochemical Co., Ltd., production batch number 0424070005) 5.88 parts, vitamin D3 (purchased from Zhejiang Weishi Biotechnology Co., Ltd., production batch number 2936290090) 0.3 parts, vitamin B2 (purchased from Shanghai Haijianuo Pharmaceutical Development Co., Ltd., production batch number HS230909) 2.5 parts, vitamin C (purchased from Shandong Luwei Pharmaceutical Co., Ltd., production batch number 2240123009) 90 parts, homolanine selenium (purchased from British Union Pumexin Technology (Jiangxi) Co., Ltd., brand Pumexin Yingte Selenium) ), 3.5 parts of yucca extract (purchased from Xi'an Nuozhong Kangjian Biotechnology Co., Ltd., production batch number C25022101) 15 parts, niacinamide 20.5 parts, xylo-oligosaccharides (purchased from Shandong Longli Biotechnology Co., Ltd., production batch number F95P20241036) 15 parts, urea 50 parts, sodium bicarbonate 10 parts, Brido 695 (polyethylene glycol glycerol ricinoleate, polyethylene glycol chain polymerization degree 26, purchased from Nouryon) 130 parts, Brido 694 (polyethylene glycol glycerol ricinoleate, polyethylene glycol chain polymerization degree 36, purchased from Nouryon) 15 parts, propylene glycol 35 parts, sodium metabisulfite 1.5 parts, disodium ethylenediaminetetraacetic acid (EDTA-2Na) 0.3 parts, and the volume was adjusted to 1 L with purified water.

[0036] This embodiment also provides a method for preparing a composite nanoemulsion, comprising the following steps: (1) Mix vitamin A palmitate, vitamin E acetate and vitamin D3, heat to 50-60℃, stir uniformly at 300-500 r / min, add Brij 695, continue to stir until uniform, then slowly add 15-20% purified water, continue to stir until clear and transparent, to make the first emulsion for standby.

[0037] (2) Mix Yucca extract and Brij 694, stir uniformly, then add propylene glycol and 2-5% purified water, stir uniformly, to make the second emulsion for standby.

[0038] (3) Dissolve urea in the remaining purified water at 20℃, after complete dissolution, add sodium bicarbonate, vitamin B2 in turn, stir thoroughly, then add nicotinamide, finally add vitamin C, selenomethionine, EDTA-2Na, sodium pyrosulfite and xylo-oligosaccharide in turn, stir thoroughly until completely dissolved, to make the water phase for standby.

[0039] (4) Mix the first emulsion, the second emulsion and the water phase, stir at room temperature (25℃) at 300-500 r / min for 10-15 minutes, to make the composite nanoemulsion.

[0040] Example 2: This example is substantially the same as Example 1, except that the addition amount of vitamin B2 in the composite nanoemulsion is different, which is 3.5 parts.

[0041] Example 3: This example is substantially the same as Example 1, except that the composite nanoemulsion does not contain xylo-oligosaccharide.

[0042] Comparative Example 1: This comparative example is substantially the same as Example 1, except that the composite nanoemulsion does not contain nicotinamide.

[0043] Comparative Example 2: This comparative example is substantially the same as Example 1, except that the addition amount of nicotinamide in the composite nanoemulsion is different, which is 10 parts.

[0044] Comparative Example 3: This comparative example is substantially the same as Example 1, except that the composite nanoemulsion does not contain urea.

[0045] Comparative Example 4: This comparative example is substantially the same as Example 1, except that the addition amount of urea in the composite nanoemulsion is different, which is 30 parts.

[0046] Comparative Example 5: This comparative example is substantially the same as Example 1, except that the addition amounts of Brij 695, Brij 694 and propylene glycol in the composite nanoemulsion are different. Among them, Brij 695 is 100 parts, Brij 694 is 5 parts, and propylene glycol is 10 parts.

[0047] Comparative Example 6: This comparative example is substantially the same as Example 1, except that an equal amount of methionine selenium is used in place of homolanolinic acid selenium in the composite nanoemulsion.

[0048] Comparative Example 7: This comparative example is substantially the same as Example 1, except that the composite nanoemulsion does not contain selenium homolanolinate, yucca extract, niacinamide, and xylo-oligosaccharide.

[0049] Performance testing: 1. Appearance: Observe the appearance and transparency of the sample while it is still.

[0050] The appearance results of the above examples and comparative examples are shown in Table 1.

[0051]

[0052] The product prepared in the embodiment of the present invention has a qualified appearance, while the product prepared in the comparative example fails to form a nanoemulsion.

[0053] 2. Particle size: Use a laser particle size distribution analyzer to dilute the nanoemulsion to the concentration required for the laser particle size distribution analyzer test, and calculate the particle size distribution and average particle size by measuring light scattering.

[0054] The particle sizes of the above examples are shown in Table 2.

[0055]

[0056] The nanoemulsion prepared in the embodiment of the present invention has a small particle size and a narrow particle size distribution, and can be absorbed more quickly, thereby reducing the degradation and loss of active ingredients in the gastrointestinal tract, and helping to improve the bioavailability of the nanoemulsion.

[0057] 3. Physical stability: 1) Centrifugation experiment: The nanoemulsion was diluted 5 times with deionized water at room temperature and placed in a centrifuge. The samples were centrifuged at 3000 r / min for 15 min, 30 min, and 45 min, respectively, to observe whether the samples had stratification or precipitation.

[0058] The centrifugal test results of the above embodiments and comparative examples are shown in Table 3.

[0059]

[0060] The nanoemulsion prepared in Example 1 of the present invention has good centrifugal stability. However, the product of the comparative example showed precipitation or stratification after centrifugation, indicating poor centrifugal stability.

[0061] 2) Dilution stability: The nanoemulsion was diluted at various times to observe its stability. Specifically, the nanoemulsion was diluted 10-fold, 20-fold, and 30-fold, and the particle size and polymer dispersibility index (PDI) were measured using a laser particle size analyzer. A change in average particle size before and after dilution of ≤10% and a PDI of ≤0.2 after dilution indicated good dilution stability.

[0062] Table 4 below shows the average particle size and PDI changes of the nanoemulsion of Example 1 at different dilution ratios.

[0063]

[0064] It can be seen that the average particle size of the nanoemulsion of Example 1 is almost unchanged when diluted 10 to 30 times, and the PDI also changes slightly, indicating that the nanoemulsion of Example 1 has good dilution stability.

[0065] The same method was used to examine the nanoemulsions of Example 2 and Example 3, respectively, and similar experimental results were obtained.

[0066] 3) High temperature test: The nanoemulsion was placed at 25°C, 40°C, and 60°C, and 75% humidity, and observed in the dark for 5 days and 10 days, respectively. The particle size and PDI were measured using a laser particle size analyzer and compared with the particle size and PDI before heat treatment. The smaller the change, the better the storage stability. When the average particle size change before and after heat treatment is ≤10% and the PDI after dilution is ≤0.2, it indicates that the nanoemulsion has good heat treatment stability.

[0067] Table 5 below shows the average particle size and PDI changes of the nanoemulsion of Example 1 at different temperatures.

[0068]

[0069] As can be seen, the nanoemulsion of Example 1 exhibits minimal changes in average particle size and PDI at room temperature, demonstrating its excellent stability at room temperature. However, it is sensitive to temperature. At 40°C, 60°C, and high humidity, aggregation increases over time, leading to increased particle size and increased risk. It should be noted that the average particle size at day 0 shown here differs slightly from that in Table 2, likely due to measurement errors.

[0070] The same method was used to examine the nanoemulsions of Example 2 and Example 3, respectively, and similar experimental results were obtained.

[0071] 4) Low temperature test: The nanoemulsion was placed in a 4°C refrigerator for 20 days and 40 days, and its appearance, particle size and PDI were observed. When the average particle size change before and after low temperature treatment was ≤10% and the PDI after dilution was ≤0.2, it indicated that the nanoemulsion had good low temperature stability.

[0072] Table 6 below shows the average particle size and PDI changes of the nanoemulsion of Example 1 under low temperature test.

[0073]

[0074] It can be seen that the average particle size and PDI of the nanoemulsion of Example 1 did not change significantly after being placed at low temperature for 20 days or even 40 days, indicating that the nanoemulsion has good low-temperature stability.

[0075] The same method was used to examine the nanoemulsions of Example 2 and Example 3, respectively, and similar experimental results were obtained.

[0076] 4. Chemical stability: 1) High temperature test: The nanoemulsions were stored at 25°C, 40°C, and 60°C in the dark for 30 days, and the retention rates (g / L) of vitamin A palmitate and vitamin E acetate in the nanoemulsions were measured. Vitamin A palmitate and vitamin E acetate were detected by Agilent HPLC using pure methanol as the mobile phase for vitamin A palmitate and methanol + purified water as the mobile phase for vitamin E acetate. The detection conditions were similar to those in existing technologies.

[0077] Table 7 below shows the changes in the retention rates (g / L) of vitamin A palmitate and vitamin E acetate in the nanoemulsion of Example 1 at different temperatures.

[0078]

[0079] Note: Some of the measured values ​​of vitamin A palmitate in the table are higher than 5.88 g / L, which may be a measurement error. It is generally considered that 96%~104% of the labeled amount is normal.

[0080] It can be seen that the vitamin A palmitate and vitamin E acetate of the nanoemulsion of Example 1 did not undergo significant changes at different temperatures, indicating that the nanoemulsion had good chemical stability.

[0081] The same method was used to examine the nanoemulsions of Example 2 and Example 3, respectively, and similar experimental results were obtained.

[0082] The nanoemulsion was stored in the dark at 40° C. for 1 month, 3 months, and 6 months, and samples were taken regularly to investigate the retention rate of vitamin B2 in the nanoemulsion. The detection of vitamin B2 can be carried out by fluorescence spectrophotometry, and the detection conditions can refer to the existing technology.

[0083] The test results of each embodiment are shown in Table 8 below.

[0084]

[0085] It can be seen that even at high temperature (40°C), the vitamin B2 retention rate of the nanoemulsion of the embodiment of the present invention hardly changes significantly after longer storage, indicating that the nanoemulsion has good storage stability even at high temperature.

[0086] 2) Shelf life: Store the nanoemulsion in a sealed container at room temperature (25°C) away from light. Regularly test the nanoemulsion for appearance, particle size, zeta potential and other indicators. Observe whether there is any stratification, precipitation, or deterioration. The shelf life of the nanoemulsion should be determined until it no longer meets the quality requirements.

[0087] The performance test data of the above embodiments and comparative examples are summarized in Table 9 below.

[0088]

[0089] It can be seen that the nanoemulsion of the embodiment of the present invention has a longer shelf life.

[0090] This invention not only has a high active ingredient content of nearly 20%, but also achieves efficient co-delivery of multiple active ingredients, including vitamin E acetate, vitamin A palmitate, vitamin D3, vitamin B2, vitamin C, homolanine selenium, yucca extract, niacinamide, and xylo-oligosaccharides. This unique co-delivery method significantly enhances the product's functional density and application value, giving it broad application prospects in related fields.

[0091] Furthermore, by optimizing the formulation, the nanoemulsion exhibits a clear, transparent solution with a small, narrowly distributed particle size and excellent physical and chemical stability. These properties make the product more convenient and reliable during storage, transportation, and use, further enhancing its market competitiveness.

[0092] Comparison of Example 1 and Comparative Example 1 reveals that without niacinamide, nanoemulsions cannot form when multiple active ingredients, such as vitamin B2, are added. This is likely because niacinamide is an amphiphilic molecule, containing a hydrophilic amide group and a hydrophobic pyridine ring in its molecular structure. This unique structure enables niacinamide to adsorb at the oil-water interface, effectively reducing interfacial tension and promoting the formation and stability of nanoemulsion droplets. Furthermore, the amide group of niacinamide can form a hydrogen bond network with other components in the system (such as vitamin B2), significantly enhancing the mechanical strength of the interfacial film. Its pyridine ring may interact with hydrophobic components (such as vitamin E) through π-π stacking, further stabilizing the droplet structure. However, insufficient niacinamide dosage also fails to form a stable nanoemulsion (as shown in Comparative Example 2). Excessive dosage can adversely affect the system. Therefore, the niacinamide content in the nanoemulsion is preferably 15-40 g / L, more preferably 15-30 g / L.

[0093] By comparing Example 1 and Comparative Example 3, it can be found that if urea is not added in the formula, nanoemulsion cannot be formed even if multiple active components such as vitamin B2 are added. This may be because urea and nicotinamide synergistically promote the dissolution of components such as vitamin B2 under weak alkaline conditions to facilitate their dispersion in the medium, effectively reducing droplet aggregation. At the same time, urea can indirectly maintain the conformation of some macromolecules (such as yucca extract) to prevent them from aggregating due to hydrophobic interactions and weaken the negative charge of homoarginine selenium. In addition, urea can also synergize with other components in the system to maintain the stability of the nanoemulsion system. However, if the amount of urea is insufficient, stable nanoemulsion cannot be formed (as shown in Comparative Example 2); and if the amount is too high, it will have an adverse effect on the system. Therefore, the content of urea in the nanoemulsion is preferably 40-60 g / L, and further preferably 45-55 g / L.

[0094] In addition, the emulsifier and cosolvent are also critical in the present application. When the amount of emulsifier and cosolvent is not appropriate (such as Comparative Example 5), not only can nanoemulsion not be formed, but the stability of the system will also be greatly compromised. The choice of organic selenium is also important. When methionine selenium is used, it is not conducive to the formation of a stable nanoemulsion system because methionine selenium has poor solubility in the aqueous phase.

[0095] Application test: 300 12-day-old AA white-feathered broilers were randomly divided into three representative groups, namely the control group, test group 1 and test group 2. The test chickens were raised in a 4-layer stacked cage, with free access to water and feed throughout the experiment. The control group was fed a basic diet, and test groups 1 and 2 were fed a basic diet supplemented with the nanoemulsion of Example 1 and Comparative Example 7, respectively, at a dosage of 500 g / t of diet. The experiment lasted for 14 days, including a 7-day pre-test period and a 7-day main test period. During the experiment, the feeding troughs were emptied before feeding each day, and then the feed was fed to ensure that the diet was clean.

[0096] After the experiment, the indicators were measured, and the test results are shown in Table 10. Among them: Daily average weight gain = (total weight of chickens at the end of the group - initial total weight of chickens in the group) / (number of test days x total number of chickens).

[0097] Daily average feed intake = total feed intake of chickens in the group / (number of test days x total number of chickens).

[0098] Feed conversion rate = daily feed intake / daily weight gain. The lower the feed conversion rate, the less feed is required per unit of weight gain, and the higher the breeding efficiency.

[0099] Survival rate = [(total number of chickens in the group - number of dead chickens in the group) / total number of chickens in the group] x 100%.

[0100] Uniformity = the number of qualified individuals / the total number of samples × 100%, according to the scale of the chicken population, the sample is proportionally extracted, and the sample is not less than 50 at least, and the qualified sample is defined as: the chicken with the weight in the range of the average weight of the whole group ± 10% is regarded as qualified.

[0101]

[0102] It can be seen that the nanoemulsion of the embodiment of the present application has the best broiler growth rate and feed conversion rate, and the survival rate and uniformity of the broilers are higher. It is illustrated that the nanoemulsion of the present application can effectively promote the healthy growth of the broilers, and has important application value in improving the breeding benefit.

[0103] The present application is described in detail above, the purpose is to let the person who is familiar with this field technology can understand the content of the present application and implement, and cannot limit the protection scope of the present application, all equivalent changes or modifications according to the spirit of the present application should be covered in the protection scope of the present application.

Claims

1. A composite nanoemulsion, characterized in that Each liter of composite nanoemulsion includes: 35g~50g fat-soluble vitamins, 85g~100g water-soluble vitamins, 2g~5g homolanine selenium, 5g~25g yucca extract, 15g~40g niacinamide, 40g~60g urea, 140g~160g emulsifier, 25g~45g cosolvent and 8g~15g stabilizer, wherein: The fat-soluble vitamins include vitamin A, vitamin D and vitamin E; The water-soluble vitamins include vitamin B and vitamin C; The emulsifier is selected from at least two polyethylene glycol glycerol ricinoleates, wherein the polyethylene glycol chain polymerization degree of one polyethylene glycol glycerol ricinoleate is 31-40, and the polyethylene glycol chain polymerization degree of the other polyethylene glycol glycerol ricinoleate is 20-30, and the mass ratio of the polyethylene glycol glycerol ricinoleate with a polyethylene glycol chain polymerization degree of 20-30 to the polyethylene glycol glycerol ricinoleate with a polyethylene glycol chain polymerization degree of 31-40 is (3-12):

1.

2. The composite nanoemulsion according to claim 1, characterized in that The content of vitamin A in the composite nanoemulsion is 4-7 g / L; And / or, the content of vitamin D in the composite nanoemulsion is 0.1-0.5 g / L; And / or, the content of vitamin E in the composite nanoemulsion is 30-40 g / L; And / or, the content of vitamin B in the composite nanoemulsion is 2-4 g / L; And / or, the content of vitamin C in the composite nanoemulsion is 80-100 g / L.

3. The composite nanoemulsion according to claim 1, characterized in that The vitamin A is vitamin A palmitate; And / or, the vitamin D is vitamin D3; And / or, the vitamin E is vitamin E acetate; And / or, the vitamin B is vitamin B2.

4. The composite nanoemulsion according to claim 1, characterized in that The cosolvent is selected from one or more of polyethylene glycol and propylene glycol; And / or, the stabilizer is sodium metabisulfite, sodium bicarbonate and ethylenediaminetetraacetate.

5. The composite nanoemulsion according to claim 4, characterized in that The content of sodium metabisulfite in the composite nanoemulsion is 0.5-2.5 g / L; and / or, the content of sodium bicarbonate in the composite nanoemulsion is 8-12 g / L; And / or, the content of EDTA in the composite nanoemulsion is 0.1-0.5 g / L.

6. The composite nanoemulsion according to claim 1, characterized in that The average particle size of the composite nanoemulsion does not exceed 50 nm.

7. The composite nanoemulsion according to claim 6, characterized in that The average particle size of the composite nanoemulsion is 40 nm to 50 nm.

8. The composite nanoemulsion according to claim 1, characterized in that Each liter of the composite nanoemulsion comprises: 4g-7g vitamin A, 0.1g-0.5g vitamin D, 30g-40g vitamin E, 2g-4g vitamin B, 80g-100g vitamin C, 2g-5g homolanine selenium, 10g-20g yucca extract, 15g-25g niacinamide, 45g-55g urea, 110g-120g polyethylene glycol glycerol ricinoleate with a polyethylene glycol chain polymerization degree of 20-30, 10g-20g polyethylene glycol glycerol ricinoleate with a polyethylene glycol chain polymerization degree of 31-40, 25g-45g solubility aid, 8g-12g sodium bicarbonate, 0.5g-2.5g sodium metabisulfite and 0.1g-0.5g ethylenediaminetetraacetate.

9. The composite nanoemulsion according to claim 1 or 8, characterized in that The composite nanoemulsion further comprises 10g to 20g of xylooligosaccharide.

10. The composite nanoemulsion according to claim 1, characterized in that The dispersion medium of the composite nanoemulsion is water.

11. The method for preparing a composite nanoemulsion according to any one of claims 1 to 10, wherein: The steps include: (1) After mixing the fat-soluble vitamins, polyethylene glycol glycerol ricinoleate with a polyethylene glycol chain polymerization degree of 20 to 30 and a portion of the dispersion medium are added and mixed uniformly to prepare a first mixed solution; (2) mixing the yucca extract with polyethylene glycol glycerol ricinoleate having a polyethylene glycol chain polymerization degree of 31 to 40, adding a cosolvent and a portion of a dispersing medium, and mixing them uniformly to prepare a second mixed solution; (3) dissolving urea in the remaining dispersion medium, adding a stabilizer, water-soluble vitamins, niacinamide, and selenium homolanolinate, and mixing them evenly to prepare a third mixed solution; (4) Evenly mixing the first mixed solution, the second mixed solution, and the third mixed solution to prepare the composite nanoemulsion.

12. The use of the composite nanoemulsion according to any one of claims 1 to 10, characterized in that: The application includes adding the composite nanoemulsion into feed or drinking water as a feed additive.

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