Moisturizing, anti-inflammatory and anti-allergy sodium hyaluronate composition and application thereof

Abstract

The invention relates to a moisturizing and anti-inflammatory composition, in particular to a moisturizing, anti-inflammatory and anti-allergy sodium hyaluronate composition. The sodium hyaluronate composition is prepared from the following components in percentage by mass: 1 to 15 percent of small molecular weight sodium hyaluronate, 3 to 8 percent of traditional Chinese medicine extract, 1 to 3 percent of polypeptide, 5 to 10 percent of ethanol, 0.5 to 2 percent of vitamin C, 0.1 to 0.5 percent of thickening agent and the balance of deionized water, the traditional Chinese medicine extract is prepared from petroselinum crispum, rosa roxburghii tratt and petasites tatewakianus; the molecular weight of the sodium hyaluronate with the small molecular weight is 2000 to 5000 Da. The moisturizing, anti-inflammatory and anti-allergy sodium hyaluronate composition disclosed by the invention adopts natural traditional Chinese medicine extracts and sodium hyaluronate with small classifications as main raw materials, has multiple effects of moisturizing, anti-inflammatory, anti-allergy and the like, is safe and does not have side effects.

Description

Technical Field

[0001] The present invention relates to a moisturizing and anti-inflammatory composition, and in particular to a moisturizing, anti-inflammatory and allergy-relief sodium hyaluronate composition. The present invention also relates to a preparation method and application of the composition. Background Art

[0002] With changes in living environments, increasing social pressures, and the frequent use of various cosmetics, the number of people suffering from skin allergies or sensitivities is increasing. People with allergies or sensitivities are prone to symptoms such as acne, redness, itching, and stinging, as well as dryness and flaking. Currently, there are a wide variety of anti-allergic products on the market, but their effectiveness varies. Most medicines and skincare products contain numerous chemical additives, which themselves have certain side effects on human skin. Some products even use hormones and antibiotics. While these products may be effective in the short term, they are prone to recurrence.

[0003] Sodium hyaluronate is a natural, high-molecular-weight mucopolysaccharide known as a "natural moisturizing factor." Research has shown that sodium hyaluronate has important physiological functions, including improving skin hydration, preventing postoperative adhesions, promoting wound healing, and providing anti-inflammatory and anti-tumor properties. Consequently, it is widely used in the medical, cosmetic, and food industries. Therefore, sodium hyaluronate is a key ingredient in moisturizing and anti-inflammatory products. Some existing skin care products use sodium hyaluronate as a primary ingredient, but these sodium hyaluronates have a relatively high molecular weight, uneven distribution, and limited anti-allergic effects. Summary of the Invention

[0004] In response to the above technical problems, the present invention provides a moisturizing, anti-inflammatory and soothing sodium hyaluronate composition. The composition uses natural Chinese medicine extracts as the main raw materials and adds fewer chemical substances. It has multiple functions such as anti-inflammatory, moisturizing, and soothing, and is safe and has no side effects.

[0005] In a first aspect, the present invention provides a moisturizing, anti-inflammatory and soothing sodium hyaluronate composition, which is composed of the following components in percentage by mass: 1-15% low molecular weight sodium hyaluronate, 3-8% traditional Chinese medicine extract, 1-3% polypeptide, 5-10% ethanol, 0.5-2% vitamin C, 0.1-0.5 thickener, and the balance is deionized water; the traditional Chinese medicine extract is made from parsley, rowan herb and butterbur; the molecular weight of the low molecular weight sodium hyaluronate is 2000-5000 Da.

[0006] As a preferred embodiment, the Chinese medicine extract is prepared from 15 to 30 parts of Herba Cynanchifoliae, 10 to 40 parts of Parsley and 10 to 30 parts of Butterbur.

[0007] As a preferred embodiment, the Chinese medicine extract is prepared from 20-25 parts of Herba Cynanchifoliae, 20-30 parts of Parsley and 20-30 parts of Butterbur.

[0008] As a preferred embodiment, the mass percentage of the vitamin C is 1.0-1.5%. As a preferred embodiment, the thickener includes but is not limited to a combination of one or more of xanthan gum, high molecular weight polyhydrate, coconut oil, pectin, carbomer and sodium alginate.

[0009] In a second aspect, the present invention provides a method for preparing the above-mentioned moisturizing, anti-inflammatory and soothing sodium hyaluronate composition, comprising the following steps: (1) Crush the dried celery, rowan herb, and butterbur separately and set aside; (2) The crushed material is added to water and soaked, and then ultrasonically extracted at 70-85°C for 40-70 minutes, and filtered to obtain the extract and filter residue; (3) Add the filter residue to an ethanol-water solution, perform ultrasonic extraction at 35-50°C for 2-3 hours, and filter the extracted mixture to obtain an extract; (4) combining the extract obtained in step (2) and the extract obtained in step (3), evaporating the solvent, and obtaining a Chinese herbal medicine extract; (5) Add low molecular weight sodium hyaluronate, Chinese herbal extract, and vitamin C to deionized water according to the formula amount and mix evenly; add the polypeptide to ethanol and stir to dissolve, then mix the two thoroughly, and then add the thickener and stir evenly.

[0010] As a preferred embodiment, in step (1), the parsley, rowan herb and butterbur are crushed to 20-30 mesh.

[0011] As a preferred embodiment, in step (2), the mixture is soaked in water for 3 to 4 hours.

[0012] As a preferred embodiment, in step (2), immersion is performed with 4-6 times the volume of distilled water.

[0013] As a preferred embodiment, in step (3), the concentration of the ethanol aqueous solution is 75-90%.

[0014] As a preferred embodiment, in step (3), the material-liquid ratio of the filter residue to the ethanol aqueous solution is 1:15~30g / mL.

[0015] In a third aspect, the present invention provides use of a moisturizing, anti-inflammatory and soothing sodium hyaluronate composition in the preparation of skin care products.

[0016] The application includes, but is not limited to, one of a mask, a skin cream, a facial cleanser, a toner, a skin lotion, a sunscreen, a BB cream, a shampoo, a shower gel and a skin jelly.

[0017] Compared with the prior art, the present application has the beneficial effects that: 1. The moisturizing anti-inflammatory soothing sodium hyaluronate composition of the present application uses natural traditional Chinese medicine extracts and small molecular weight sodium hyaluronate as main raw materials, and adds less chemicals, has multiple effects such as moisturizing, anti-inflammatory, soothing and the like, and is safe and has no side effects.

[0018] 2. The moisturizing anti-inflammatory soothing sodium hyaluronate composition of the present application contains sodium hyaluronate with a molecular weight of 2000-5000 Da, and only sodium hyaluronate with an average molecular weight of less than 5000 Da can penetrate into the inside of the dermis layer, moisturize the deep layer, and truly inhibit inflammation and repair cells. The present application uses 2000-5000 Da sodium hyaluronate with uniform molecular weight distribution as the main component, changes the original mode of using sodium hyaluronate as a moisturizing agent auxiliary material, and plays a good role in moisturizing and anti-inflammatory.

[0019] The main components of the Luo Wen grass extract are eucalyptol, monoterpenes, monoterpenols, phenolic chemicals and polysaccharides, have the effects of promoting blood circulation, repairing skin, relieving skin redness, and the like, the phenolic compounds have soothing effect on sensitive skin, the polysaccharides can promote cell repair and regeneration, enhance skin barrier function, and improve the overall health status of the skin.

[0020] The main components of the carrot extract include apigenin, volatile oil, bergamot inner fat ester, organic acid, protein and various vitamins, have the effects of anti-inflammatory soothing, moisturizing and improving dry problems, the volatile oil and the organic acid play a role in inhibiting the growth and reproduction of bacteria; the apigenin has antioxidant performance, can remove free radicals and prevent skin from getting acne and aging.

[0021] The Pteridium aquilinum extract can effectively inhibit the release of histamine and reduce or inhibit allergic symptoms, and is rich in various antioxidant components, which can reduce the damage of oxidative stress to the skin.

[0022] Vitamin C is a natural antioxidant and has good antioxidant and free radical scavenging capacity.

[0023] The polypeptide can penetrate into the deep layer of the skin to play a moisturizing role, and on the other hand, interfere with the release and activation of inflammatory mediators to reduce inflammation, and together with the Pteridium aquilinum extract, plays a role in anti-inflammatory and anti-allergic.

[0024] The combination of the various drugs achieves the effects of moisturizing, anti-inflammatory, soothing and the like.

[0025] 3. The preparation method of the sodium hyaluronate composition of the present invention is carried out with appropriate extraction method, time and temperature, which is more conducive to retaining the active ingredients of the drug. The preparation method is simple and has better therapeutic effect. DETAILED DESCRIPTION

[0026] The technical solution of the present invention will be further described in detail below in conjunction with specific implementation methods, but this does not constitute any limitation to the present invention.

[0027] In Example 1, 15 g of dried celery, 10 g of rowan herb, and 10 g of butterbur are respectively crushed into 20-30 mesh sizes; the crushed materials are added to 4-6 times the volume of water and soaked for 3-4 hours, then ultrasonically extracted at 70-85° C. for 40-70 minutes, and filtered to obtain an extract and a filter residue; the filter residue is added to an ethanol aqueous solution with a solid-liquid ratio of 1:15-30 g / mL and a concentration of 75-90%, ultrasonically extracted at 35-50° C. for 2-3 hours, and the extracted mixture is filtered to obtain an extract; the two extracts are combined, and the solvent is evaporated to obtain a traditional Chinese medicine extract.

[0028] According to the mass ratio, 1% of small molecule sodium hyaluronate with a molecular weight of 5000Da, 8% of traditional Chinese medicine extract, and 1% of vitamin C are added to 83.9% of deionized water and mixed evenly; 1% of polypeptide is added to 5% of ethanol and stirred to dissolve, and then the two are fully mixed, and then 0.1% of thickener is added and stirred evenly to obtain a moisturizing, anti-inflammatory and soothing sodium hyaluronate composition.

[0029] In Example 2, 30 g of dried celery, 40 g of rowan herb, and 30 g of butterbur were respectively crushed into 20-30 mesh sizes; the crushed materials were added to 4 times the volume of water and soaked for 3 hours, and then ultrasonically extracted at 70° C. for 70 minutes, and filtered to obtain an extract and a filter residue; the filter residue was added to an ethanol aqueous solution with a solid-liquid ratio of 1:30 g / mL and a concentration of 75%, and ultrasonically extracted at 50° C. for 2 hours. The extracted mixture was filtered to obtain an extract; the two extracts were combined, and the solvent was evaporated to obtain a traditional Chinese medicine extract.

[0030] 15% of small molecule sodium hyaluronate with a molecular weight of 3000Da, 3% of traditional Chinese medicine extract, and 1.5% of vitamin C are added to 67% of deionized water and mixed evenly according to the mass ratio; 3% of polypeptide is added to 10% ethanol and stirred to dissolve, and then the two are fully mixed, and then 0.5% of thickener is added and stirred evenly to obtain a moisturizing, anti-inflammatory and soothing sodium hyaluronate composition.

[0031] Example 3, dry 20g of cumin, 20g of lovage and 20g of Pteridium aquilinum were respectively crushed to 20-30 mesh; the crushed materials were added to 6 times the volume of water and soaked for 3 hours, then ultrasonic extraction was carried out at 85°C for 40 min, and the filtrate and residue were obtained by filtration; the residue was added to an ethanol aqueous solution with a concentration of 90% and a solid-liquid ratio of 1:15 g / mL, and ultrasonic extraction was carried out at 35°C for 2 hours, and the mixture after extraction was filtered to obtain the extract; the two extract solutions were combined, and the solvent was evaporated to obtain the traditional Chinese medicine extract.

[0032] According to the mass ratio, 8% small molecular weight hyaluronic acid sodium with a molecular weight of 2000 Da, 5% traditional Chinese medicine extract, and 0.5% vitamin C were added to 76.2% deionized water and mixed uniformly; 2% polypeptide was dissolved in 8% ethanol by stirring, then the two were fully mixed, and 0.3% thickening agent was added and stirred uniformly to obtain a hyaluronic acid sodium composition with moisturizing, anti-inflammatory and soothing effects.

[0033] Example 4, dry 25g of cumin, 30g of lovage and 30g of Pteridium aquilinum were respectively crushed to 20-30 mesh; the crushed materials were added to 5 times the volume of water and soaked for 3 hours, then ultrasonic extraction was carried out at 80°C for 60 min, and the filtrate and residue were obtained by filtration; the residue was added to an ethanol aqueous solution with a concentration of 90% and a solid-liquid ratio of 1:25 g / mL, and ultrasonic extraction was carried out at 40°C for 3 hours, and the mixture after extraction was filtered to obtain the extract; the two extract solutions were combined, and the solvent was evaporated to obtain the traditional Chinese medicine extract.

[0034] According to the mass ratio, 10% small molecular weight hyaluronic acid sodium with a molecular weight of 5000 Da, 5% traditional Chinese medicine extract, and 1% vitamin C were added to 77.7% deionized water and mixed uniformly; 1% polypeptide was dissolved in 5% ethanol by stirring, then the two were fully mixed, and 0.3% thickening agent was added and stirred uniformly to obtain a hyaluronic acid sodium composition with moisturizing, anti-inflammatory and soothing effects.

[0035] Comparative Example 1, according to the mass ratio, 10% small molecular weight hyaluronic acid sodium with a molecular weight of 2000-5000 Da, 1% vitamin C were added to 82.7% deionized water and mixed uniformly; 1% polypeptide was dissolved in 5% ethanol by stirring, then the two were fully mixed, and 0.3% thickening agent was added and stirred uniformly to obtain a hyaluronic acid sodium composition with moisturizing, anti-inflammatory and soothing effects.

[0036] In comparative example 2, 30 g of dried radix serratae and 30 g of butterbur were respectively crushed into 20-30 mesh; the crushed materials were added to 5 times the volume of water and soaked for 3 hours, and then ultrasonically extracted at 80° C. for 60 minutes, and filtered to obtain an extract and a residue; the residue was added to an ethanol aqueous solution with a solid-liquid ratio of 1:25 g / mL and a concentration of 90%, and ultrasonically extracted at 40° C. for 3 hours. The extracted mixture was filtered to obtain an extract; the above two extracts were combined, and the solvent was evaporated to obtain a traditional Chinese medicine extract.

[0037] 10% of small molecule sodium hyaluronate with a molecular weight of 2000~5000Da, 5% of traditional Chinese medicine extract, and 1% of vitamin C are added to 77.7% of deionized water and mixed evenly according to the mass ratio; 1% of polypeptide is added to 5% of ethanol and stirred to dissolve, and then the two are fully mixed, and then 0.3% of thickener is added and stirred evenly to obtain a moisturizing, anti-inflammatory and soothing sodium hyaluronate composition.

[0038] In comparative example 3, 25 g of dried celery and 30 g of butterbur were respectively crushed into 20-30 mesh; the crushed materials were added to 5 times the volume of water and soaked for 3 hours, and then ultrasonically extracted at 80° C. for 60 minutes, and filtered to obtain an extract and a residue; the residue was added to an ethanol aqueous solution with a solid-liquid ratio of 1:25 g / mL and a concentration of 90%, and ultrasonically extracted at 40° C. for 3 hours. The extracted mixture was filtered to obtain an extract; the above two extracts were combined, and the solvent was evaporated to obtain a traditional Chinese medicine extract.

[0039] 10% of small molecule sodium hyaluronate with a molecular weight of 2000~5000Da, 5% of traditional Chinese medicine extract, and 1% of vitamin C are added to 77.7% of deionized water and mixed evenly according to the mass ratio; 1% of polypeptide is added to 5% of ethanol and stirred to dissolve, and then the two are fully mixed, and then 0.3% of thickener is added and stirred evenly to obtain a moisturizing, anti-inflammatory and soothing sodium hyaluronate composition.

[0040] In comparative example 4, 25 g of dried celery and 30 g of rowan herb were respectively crushed into 20-30 mesh; the crushed materials were added to 5 times the volume of water and soaked for 3 hours, and then ultrasonically extracted at 80°C for 60 minutes, and filtered to obtain an extract and a residue; the residue was added to an ethanol aqueous solution with a solid-liquid ratio of 1:25 g / mL and a concentration of 90%, and ultrasonically extracted at 40°C for 3 hours. The extracted mixture was filtered to obtain an extract; the above two extracts were combined, the solvent was evaporated, and a traditional Chinese medicine extract was obtained.

[0041] 10% of small molecule sodium hyaluronate with a molecular weight of 2000~5000Da, 5% of traditional Chinese medicine extract, and 1% of vitamin C are added to 77.7% of deionized water and mixed evenly according to the mass ratio; 1% of polypeptide is added to 5% of ethanol and stirred to dissolve, and then the two are fully mixed, and then 0.3% of thickener is added and stirred evenly to obtain a moisturizing, anti-inflammatory and soothing sodium hyaluronate composition.

[0042] The compositions obtained in Examples 1 to 4 and Comparative Examples 1 to 4 were added to a mask bag containing mask paper and sealed to obtain a mask.

[0043] The masks were tested for physical and chemical indicators and microbial indicators, and the results are shown in Tables 1 and 2.

[0044] Table 1

[0045] Table 2

[0046] The monitoring results show that the content of heavy metals such as lead, total arsenic, and mercury, as well as various microbial indicators in the composition and facial mask provided by the present invention meet the qualified standards.

[0047] Test Example: Moisturizing Effect Test: The test conditions were a temperature of 20±2°C and a relative humidity of 50%±3%. 40 subjects aged 20-30 with healthy skin and no allergic diseases were selected. All subjects had not used hormone drugs and immunosuppressants in the past month and had not participated in other clinical trials. The subjects were divided into 4 groups, with 10 subjects in each group. The same area of ​​5cm×5cm on the inner side of the left forearm was selected as the test site. After cleaning, the facial masks prepared in Comparative Examples 1-4 and Examples 1-4 were used respectively. The skin moisture content before and 30 minutes after application of the sample was measured using a skin moisture tester. Each group of samples was tested 3 times in parallel, and the skin hydration growth rate was calculated.

[0048] The formula for calculating the growth rate of skin hydration is as follows: Skin hydration growth rate = (MWV t -MWV0) / MWV0×100%; MWV0 is the moisture content of the skin before applying the sample, MWV t The results are shown in Table 3.

[0049] Table 3

[0050] As shown in Table 3, the moisturizing effects of the facial masks prepared in Examples 1-4 significantly exceeded those of Comparative Examples 1-4. Comparative Example 1, a composition without the addition of Chinese herbal extracts, had the worst moisturizing effect, indicating that the addition of Chinese herbal extracts played a significant role in moisturizing. Comparative Example 2, which did not include the addition of parsley extract, achieved essentially the same moisturizing effect as Comparative Example 1. Comparative Examples 3-4, however, all added parsley extract and achieved essentially the same results. Therefore, compared to the Herba Rosmarinis and Herba Butterbur extracts, parsley extract is more beneficial in enhancing moisturizing properties.

[0051] Anti-allergic and antipruritic experiment: After 1 week of adaptive feeding, 70 mice were randomly divided into 7 groups: a blank control group, a histamine group, Example 1 of the present invention, and comparative examples 1-4, with 10 mice in each group. The mice were depilated with 1% sodium sulfide, leaving a 2 cm × 3 cm area on their backs. Two days later, the mice were used for the experiment. During the experiment, the corresponding sample (0.2 mL / mouse) was topically applied to the depilated area on the backs of the animals in each group for 5 consecutive days. One hour after the last dose, each group, except the blank control group, received an intradermal injection of 50 μL of histamine into the back of the neck. After histamine administration, the mice were observed for pruritus, and the number of scratching episodes within 30 minutes was recorded. The experimental data were statistically analyzed using SPSS 22.0 statistical software. Statistics were expressed as mean ± standard deviation. If the data were normally distributed, the t-test was used to analyze the changes in data before and after sample application and the differences between the test area and the matrix area. If the data were non-normally distributed, the rank sum test was used for statistical analysis, and the validity of the results was determined based on the calculated results. The results of the anti-allergic and antipruritic test are shown in Table 4.

[0052] Table 4

[0053] From the results in Table 4, it can be seen that the composition of Example 1 of the present invention has a significant anti-allergic and antipruritic effect on the pruritus caused by histamine, and there is a significant difference compared with the histamine model group, indicating that the components in the composition of the present invention have a significant synergistic anti-allergic and soothing effect. Compared with the comparative examples containing only two or no Chinese herbal extracts, its anti-allergic and antipruritic effect is significant, with a clear synergistic effect. In contrast, the anti-allergic effect of Comparative Example 4 is slightly worse, and Comparative Example 4 does not contain a Herba Butterbur extract, indicating that the Herba Butterbur extract has a more significant anti-allergic and soothing effect.

[0054] In summary, the various components of the moisturizing, anti-inflammatory and soothing sodium hyaluronate of the present invention synergistically promote each other, have significant moisturizing, anti-inflammatory and soothing effects, are safe in ingredients, are suitable for various skin care products, and are worthy of promotion and use.

[0055] The above embodiments are preferred implementations of the present invention, but the implementations of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications that do not deviate from the spirit and principles of the present invention should be considered as equivalent replacement methods and are included in the scope of protection of the present invention.

Claims

1. A moisturizing, anti-inflammatory and soothing sodium hyaluronate composition, characterized in that: The invention is composed of the following components in percentage by mass: 1-15% low molecular weight sodium hyaluronate, 3-8% traditional Chinese medicine extract, 1-3% polypeptide, 5-10% ethanol, 0.5-2% vitamin C, 0.1-0.5% thickener, and the balance is deionized water; the traditional Chinese medicine extract is made from celery, rowan herb and butterbur; the molecular weight of the low molecular weight sodium hyaluronate is 2000-5000 Da.

2. The moisturizing, anti-inflammatory and soothing sodium hyaluronate composition according to claim 1, characterized in that: The traditional Chinese medicine extract is prepared from 15 to 30 parts of rowan herb, 10 to 40 parts of celery and 10 to 30 parts of butterbur.

3. The moisturizing, anti-inflammatory and soothing sodium hyaluronate composition according to claim 2, characterized in that: The traditional Chinese medicine extract is prepared from 20-25 parts of Herba Cynanchifoliae, 20-30 parts of Parsley and 20-30 parts of Butterbur.

4. The moisturizing, anti-inflammatory and soothing sodium hyaluronate composition according to claim 1, characterized in that: The mass percentage of the vitamin C is 1.0-1.5%.

5. The moisturizing, anti-inflammatory and soothing sodium hyaluronate composition according to claim 1, characterized in that: The thickener comprises one or more combinations of xanthan gum, high molecular weight polyhydrate, coconut oil, pectin, carbomer and sodium alginate.

6. Use of the moisturizing, anti-inflammatory and soothing sodium hyaluronate composition according to claim 1 in the preparation of skin care products.

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