Airlift reactor for continuous fermentation production of bile salt hydrolase

Abstract

The invention discloses an airlift reactor for continuous fermentation production of bile salt hydrolase, and belongs to the technical field of biological fermentation equipment. The invention discloses an airlift reactor for continuous fermentation production of bile salt hydrolase. The top of the tank body is provided with a tank cover, the tank cover is provided with a feed port, the center of the inner wall of the bottom of the tank body is provided with turbulent flow columns which are uniformly distributed, and the tops of the turbulent flow columns are fixedly connected with a positioning ring; the gas distributor is mounted at the bottom of the tank body and is used for introducing dispersed gas into the tank body so as to accelerate uniform mixing of the reaction raw materials; the magnetic coupling stirring mechanism comprises a first driving assembly installed at the bottom of the tank body, through the optimal design of an adjustable inverted-cone-shaped dynamic diffusion assembly, the substrate diffusion range and mixing uniformity are remarkably improved, substrate deposition is effectively reduced, meanwhile, key components have excellent durability and sealing performance, and the service life of the device is prolonged. Therefore, the substrate utilization rate and the production efficiency of fermentation of the bile salt hydrolase are improved.

Description

Technical Field

[0001] The invention relates to the technical field of biological fermentation equipment, in particular to an airlift reactor for continuous fermentation production of bile salt hydrolase. Background Art

[0002] Airlift reactors are commonly used in the continuous fermentation production of bile salt hydrolase. However, existing airlift reactors have a limited diffusion range for substrates within the reactor, resulting in insufficient contact between the substrate and the fermenting microorganisms, affecting fermentation efficiency. Substrate easily adheres to the inner walls of the tank, causing waste and potentially breeding bacteria, impacting the quality of the fermentation product. Bubbles carry substrate with them during their rise, resulting in a high substrate loss rate. Furthermore, traditional stirring methods struggle to maintain a clean interior, hindering long-term stable operation.

[0003] Therefore, it is necessary to design a new type of airlift reactor to solve the above problems. Summary of the Invention

[0004] The present invention aims to provide an airlift reactor for continuous fermentation production of bile salt hydrolase, so as to solve the problems in the prior art such as limited diffusion range of substrate, insufficient mixing uniformity, easy deposition, and the need to improve the durability and sealing of key components, thereby improving substrate utilization and fermentation efficiency.

[0005] In order to achieve the above object, the present invention adopts the following technical solutions: An airlift reactor for continuous fermentation production of bile salt hydrolase, comprising: A tank body, wherein a tank cover is provided on the top of the tank body, a feed port is provided on the tank cover, and evenly distributed spoiler columns are provided at the center of the bottom inner wall of the tank body, and a positioning ring is fixedly connected to the top of the spoiler column; A gas distributor is installed at the bottom of the tank body and is used to introduce dispersed gas into the tank body to accelerate the mixing of the reaction raw materials; A magnetic coupling stirring mechanism, the magnetic coupling stirring mechanism includes a first driving assembly installed at the bottom of the tank body, the output end of the first driving assembly is installed with a first internal and external magnetic coupling assembly acting on the inner cavity of the tank body, the working end of the first internal and external magnetic coupling assembly placed in the inner cavity of the tank body is connected to a stirring shaft that is gap-matched with the inner side wall of the positioning ring, the bottom end of the stirring shaft is open and the inner cavity of the middle and lower sections is hollow, and the outer wall of the stirring shaft is sequentially provided with a bubble breaking assembly, a diversion assembly and a suction-type rising assembly in the vertical direction; An adjustable inverted cone-shaped dynamic diffusion component is installed between the tank cover and the top of the stirring shaft, and is used to diffuse the rising substrate along the cone surface to the edge of the tank body to increase the diffusion range of the substrate.

[0006] Preferably, the adjustable inverted cone-shaped dynamic diffusion assembly includes a second driving assembly installed at the center of the top of the tank cover, the inner wall of the top of the tank body is fixedly connected to the mounting bracket, the mounting bracket is rotatably connected to the rotating rod, and the output end of the second driving assembly is connected to the second inner and outer magnetic coupling assembly between the output end of the second driving assembly and the end of the rotating rod near the tank cover, the top of the stirring shaft is rotatably connected to the connecting seat, the outer wall of the connecting seat is rotatably connected to a uniformly distributed and inclined support rod, one end of the support rod away from the connecting seat is rotatably connected to the transmission rod, and the end of the transmission rod away from the support rod is rotatably connected to the lifting seat, the top of the lifting seat is fixedly connected to the lifting rod, and a cylindrical cam assembly is connected between the top of the lifting rod and the bottom of the rotating rod, and the outer wall of the support rod is fixedly connected to an air bag with an inverted cone structure, the air bag is placed in the umbrella frame space formed by the support rod and the transmission rod, and the air bag and the transmission rod are clearance-matched; The second drive assembly includes a second servo motor mounted on the top of the tank cover via a bracket; The second inner and outer magnetic coupling assembly includes a second outer magnetic ring fixedly mounted on the output end of the second servo motor, and the top end of the rotating rod is fixedly connected to a second inner magnetic ring arranged opposite to the second outer magnetic ring.

[0007] Preferably, the cylindrical cam assembly includes a cylinder fixedly connected to the bottom of the mounting frame through a support rod, the bottom end of the rotating rod is placed through the outer wall of the cylinder and extends to the inner cavity of the cylinder, the extended end of the rotating rod is fixedly connected to the cylindrical cam, the outer wall of the cylindrical cam is provided with a curved groove connected end to end, a slider is slidably connected in the curved groove, the outer wall of the slider is connected to a driven rod, the top end of the lifting rod passes through the outer wall of the cylinder and extends to the inner cavity of the cylinder, and the driven rod is fixedly connected to the extended end of the lifting rod.

[0008] Preferably, the top of the tank body is further provided with an air pressure balancing component for balancing the air pressure in the airbag cavity and the inner cavity of the tank body; The air pressure balancing component includes a pressure sensor installed on the inner wall of the tank body and the inner cavity of the airbag. An electrically controlled air intake valve and an electrically controlled exhaust valve are also provided on the top of the airbag. A compensation tube is also provided on the electrically controlled air intake valve. The compensation tube extends outward through the tank cover. The extended end of the compensation tube is connected to an air pressure compensation gas tank. A compensation valve is provided at the junction of the air pressure compensation gas tank and the compensation tube. The air pressure in the air pressure compensation gas tank is greater than the air pressure in the inner cavity of the tank body.

[0009] Preferably, a horizontally arranged connecting rod is fixedly connected to the outer wall of the rod end of the lifting rod close to the lifting seat, and a flexible cleaning ring that contacts the inner wall of the tank body is fixedly connected to one end of the connecting rod away from the lifting rod.

[0010] Preferably, the first driving assembly comprises a base mounted at the bottom of the tank body and having a hollow inner cavity, wherein a first servo motor is embedded in the inner cavity of the base; The first inner-outer magnetic force coupling assembly comprises a first outer magnetic ring fixedly connected to the output end of the first servo motor, and the outer lateral wall of the stirring shaft close to the bottom end is fixedly connected with a first inner magnetic ring arranged opposite to the first outer magnetic ring; The suction type rising assembly comprises a suction type paddle fixedly installed on the outer wall of the first inner magnetic ring; The flow guide assembly comprises a flow guide ring fixedly installed on the shaft wall of the middle section of the stirring shaft through a support rod, the inner lateral wall of the flow guide ring is fixedly connected with flow guide plates which are uniformly distributed and arranged in an inclined manner, and the middle section of the stirring shaft is provided with a flow guide groove which is in communication with the inner cavity of the stirring shaft and faces the flow guide plates; The bubble breaking assembly comprises stirring sub-pieces of disc-shaped structure which are installed at equal distances on the outer wall of the upper section of the stirring shaft, and the outer lateral wall of the stirring sub-pieces is provided with uniformly distributed flat sawtooth pieces, and the length of the flat sawtooth pieces decreases in a vertical direction in sequence.

[0011] Preferably, the gas distributor comprises a first annular gas pipe installed on the inner wall of the bottom of the tank body, a second annular gas pipe is arranged above the first annular gas pipe, the second annular gas pipe is provided with uniformly distributed gas injection holes in a circumferential direction, a one-way valve is connected between the first annular gas pipe and the second annular gas pipe, an air inlet pipe extending towards the bottom of the tank body is arranged on the tank cover, an electromagnetic valve is arranged on the air inlet of the air inlet pipe, one end of the air inlet pipe arranged in the tank body is connected with the first annular gas pipe, and the first annular gas pipe and the second annular gas pipe are sleeved outside the flow guide column.

[0012] Preferably, the controller is in signal connection with the magnetic force coupling stirring mechanism, the adjustable inverted cone-shaped dynamic diffusion assembly, the gas pressure balance assembly and the gas distributor.

[0013] Preferably, a low-frequency ultrasonic vibrator for destroying the adhesion of the substrate to the inner wall of the bottom of the tank body is embedded at the center of the bottom of the tank body, and the bottom of the tank body adopts a conical surface structure.

[0014] Preferably, a water cooling jacket is arranged on the outer lateral wall of the tank body, a pressure gauge, a pressure relief valve, a thermometer and a pH meter are arranged on the tank cover, a discharge port is arranged on the outer lateral wall of the tank body close to the bottom end, and a dissolved oxygen monitoring sensor is arranged on the inner lateral wall of the tank body.

[0015] Compared with the prior art, the present application provides a gas lifting type reactor for continuous fermentation production of bile salt hydrolase, which has the following beneficial effects: This airlift reactor for continuous fermentation of bile salt hydrolase utilizes an adjustable inverted cone-shaped dynamic diffusion component. The airbag contracts and expands as the ribs rise and fall. When contracted, it reduces resistance to liquid flow, facilitating the accumulation of substrate in the center. When expanded, it forms an "umbrella" that propels the substrate toward the edges of the tank, significantly increasing the diffusion range compared to a static umbrella-shaped flow cap. The pulsed deformation generated by the reciprocating rise and fall creates periodic pressure fluctuations, significantly improving the mixing efficiency of the substrate in both the radial and axial directions, further enhancing the uniformity of substrate concentration across the tank.

[0016] 2. The airlift reactor used for continuous fermentation production of bile salt hydrolase has a flexible cleaning ring connected to the lifting rod, which comes into contact with the inner wall of the tank as the lifting rod moves, thereby promptly cleaning the substrate attached to the inner wall of the tank. The dynamic movement of the airbag produces a "pumping effect", disturbing the stagnant layer near the wall of the reactor tank, reducing the deposition of substrate on the wall, and cooperating with the low-frequency ultrasonic vibrator at the bottom of the tank to further destroy the adhesion between the substrate and the tank bottom.

[0017] 3. The airlift reactor used for continuous fermentation production of bile salt hydrolase adopts magnetic coupling transmission through the magnetic coupling stirring mechanism, avoiding the leakage and contamination problems that may be caused by traditional mechanical seals and ensuring the cleanliness of the tank interior. At the same time, the rational design and coordinated operation of various components reduce the abnormal accumulation and adhesion of substrates in the tank, which is conducive to the long-term stable operation of the reactor.

[0018] 4. The airlift reactor used for continuous fermentation production of bile salt hydrolase can break bubbles during the rising process through the bubble breaking component in the magnetic coupling stirring mechanism, reducing the amount of substrate carried by the bubbles; the diversion component and the suction-type rising component can optimize the fluid flow in the tank, making the substrate distribution more uniform, and further reducing the loss rate of the substrate carried by the bubbles.

[0019] 5. The airlift reactor for continuous fermentation production of bile salt hydrolase has a pressure balance component that maintains the pressure balance between the inner cavity of the pressure tank and the inner cavity of the air bag, ensuring that the air bag deforms within the normal pressure range, avoiding damage to the air bag or affecting the substrate diffusion effect due to pressure imbalance. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 This is one of the three-dimensional structural diagrams of the present invention; Figure 2 This is the second schematic diagram of the three-dimensional structure of the present invention; Figure 3 This is one of the schematic diagrams of the internal structure of the tank body of the present invention; Figure 4 This is the second schematic diagram of the internal structure of the tank body of the present invention; Figure 5It is a front view of the magnetic coupling stirring mechanism of the present invention; Figure 6 This is a front view of the adjustable inverted cone-shaped dynamic diffusion assembly of the present invention; Figure 7 is a cross-sectional view of a cylindrical cam assembly of the present invention; Figure 8 It is a structural schematic diagram of the cylindrical cam assembly of the present invention; Figure 9 Schematic diagram of the structure of the gas distributor of the present invention; Figure 10 Schematic diagram of the structure of the first drive assembly of the present invention.

[0021] In the figure: 10, tank body; 110, tank cover; 111, mounting frame; 120, spoiler column; 130, positioning ring; 140, water-cooling jacket; 20, gas distributor; 210, first annular air pipe; 220, second annular air pipe; 230, air injection hole; 240, one-way valve; 250, air inlet pipe; 30, magnetic coupling stirring mechanism; 310, first drive assembly; 311, base; 312, first servo motor; 320, first inner and outer magnetic coupling assembly; 321, first outer magnetic ring; 322, first inner magnetic ring; 330, stirring shaft; 340, bubble crushing assembly; 341, stirring rod; 342, straight serrated blade; 350, guide assembly; 351, guide ring; 352, guide plate; 353 , guide groove; 360, suction-type paddle; 40, adjustable inverted cone-shaped dynamic diffusion component; 410, second servo motor; 420, cylindrical cam component; 421, cylinder; 422, cylindrical cam; 423, curved groove; 424, slider; 425, driven rod; 430, rotating rod; 440, second internal and external magnetic coupling component; 441, second outer magnetic ring; 442, second inner magnetic ring; 450, connecting seat; 460, support rod; 470, transmission rod; 480, lifting seat; 490, lifting rod; 491, connecting rod; 492, flexible cleaning ring; 50, airbag; 510, electronically controlled intake valve; 520, electronically controlled exhaust valve; 530, compensation pipe; 540, air pressure compensation tank; 550, compensation valve. DETAILED DESCRIPTION

[0022] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention; it is obvious that the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0023] It will be understood by those skilled in the art that, unless otherwise defined, all terms (including technical and scientific terms) used herein have the same meaning as commonly understood by those skilled in the art in the art to which the present invention pertains. It should also be understood that terms such as those defined in common dictionaries should be understood to have meanings consistent with their meanings in the context of the prior art and, unless defined as such herein, will not be interpreted in an idealized or overly formal sense.

[0024] Example: Reference Figure 1 、 Figure 2 、 Figure 3 、 Figure 4 、 Figure 5 、 Figure 6 、 Figure 7 、 Figure 8 、 Figure 9 and Figure 10 , an airlift reactor for continuous fermentation production of bile salt hydrolase, comprising: The tank body 10 is provided with a tank cover 110 on the top of the tank body 10, and a feed port is provided on the tank cover 110. The center of the bottom inner wall of the tank body 10 is provided with evenly distributed spoiler columns 120, and the top of the spoiler column 120 is fixedly connected with a positioning ring 130; The gas distributor 20 is installed at the bottom of the tank body 10 and is used to introduce dispersed gas into the tank body 10 to accelerate the mixing of the reaction raw materials; The magnetic coupling stirring mechanism 30 includes a first driving assembly 310 installed at the bottom of the tank body 10. The output end of the first driving assembly 310 is installed with a first internal and external magnetic coupling assembly 320 acting on the inner cavity of the tank body 10. The working end of the first internal and external magnetic coupling assembly 320 placed in the inner cavity of the tank body 10 is connected to a stirring shaft 330 that is gap-matched with the inner wall of the positioning ring 130. The bottom end of the stirring shaft 330 is open and the inner cavity of the middle and lower sections is hollow. The outer wall of the stirring shaft 330 is sequentially provided with a bubble crushing assembly 340, a diversion assembly 350 and a suction-type rising assembly in the vertical direction. The adjustable inverted cone-shaped dynamic diffusion component 40 is installed between the tank cover 110 and the top of the stirring shaft 330, and is used to diffuse the rising substrate along the cone surface to the edge of the tank body 10 to increase the diffusion range of the substrate.

[0025] Reference Figure 2 、 Figure 3 、 Figure 4 、 Figure 6 、 Figure 7 and Figure 8The adjustable inverted cone-shaped dynamic diffusion component 40 includes a second driving component installed at the top center of the tank cover 110. The inner wall of the top of the tank body 10 is fixedly connected to the mounting bracket 111, and the mounting bracket 111 is rotatably connected to the rotating rod 430. A second internal and external magnetic coupling component 440 is connected between the output end of the second driving component and the end of the rotating rod 430 close to the tank cover 110. The top of the stirring shaft 330 is rotatably connected to the connecting seat 450, and the outer wall of the connecting seat 450 is rotatably connected to the support rods 460 that are evenly distributed and inclined. The end of the support rod 460 away from the connecting seat 450 is rotatably connected to the transmission rod 470, and the end of the transmission rod 470 away from the support rod 460 is rotatably connected to the lifting seat. 480, a lifting rod 490 is fixedly connected to the top of the lifting seat 480, and a cylindrical cam assembly 420 is connected between the top of the lifting rod 490 and the bottom end of the rotating rod 430. An airbag 50 with an inverted cone structure is fixedly connected to the outer wall of the support rod 460. The airbag 50 is placed in the umbrella frame space formed by the support rod 460 and the transmission rod 470, and the airbag 50 and the transmission rod 470 are clearance-matched. In this embodiment, the support rod 460 and the transmission rod 470 are made of titanium alloy, and the airbag 50 is made of fluororubber with an embedded high-strength aramid fiber reinforcement layer, which is temperature-resistant to 140°C and resistant to bile salt corrosion. The connection between the support rod 460 and the airbag 50 is sealed with food-grade silicone rubber to prevent the substrate from penetrating into the interior of the airbag 50; The second drive assembly includes a second servo motor 410 mounted on the top of the tank cover 110 via a bracket. When the airbag 50 expands, it diffuses the rising substrate along the conical surface to the edge of the tank body 10, increasing the diffusion range of the substrate. When it contracts, it facilitates the aggregation of the substrate to the central area, preparing for the next diffusion. The airbag 50 folds and the ribs close, reducing the resistance to liquid flow and facilitating the aggregation of the substrate to the central area along with the rising flow. When the airbag 50 expands, the angle between the support rod 460 and the transmission rod 470 decreases, and the "umbrella surface" of the umbrella frame mechanism formed by them becomes larger, pushing the substrate to diffuse to the edge of the tank body 10. When the airbag 50 contracts, the angle between the support rod 460 and the transmission rod 470 increases, and the ribs close, reducing the resistance to liquid flow and facilitating the aggregation of the substrate to the central area along with the rising flow. The second internal and external magnetic coupling assembly 440 includes a second outer magnetic ring 441 fixedly mounted on the output end of the second servo motor 410, and the top end of the rotating rod 430 is fixedly connected to a second inner magnetic ring 442 arranged opposite to the second outer magnetic ring 441. The second servo motor 410 is mounted on the top of the tank cover 110. After starting, it drives the second outer magnetic ring 441 to rotate. The second outer magnetic ring 441 and the second inner magnetic ring 442 at the top end of the rotating rod 430 are magnetically coupled, causing the rotating rod 430 to rotate on the mounting bracket 111, and the cylindrical cam 422 connected to the bottom end of the rotating rod 430 rotates accordingly.

[0026] Reference Figure 7 and Figure 8The cylindrical cam assembly 420 comprises a cylinder 421 fixedly connected below the mounting frame 111 by a support rod, the bottom end of a rotating rod 430 extends into the inner cavity of the cylinder 421 through the outer wall of the cylinder 421, the extended end of the rotating rod 430 is fixedly connected with a cylindrical cam 422, the outer wall of the cylindrical cam 422 is provided with a curve groove 423 connected head to tail, a sliding block 424 is slidably connected in the curve groove 423, the outer wall of the sliding block 424 is connected with a driven rod 425, the top end of a lifting rod 490 extends into the inner cavity of the cylinder 421 through the outer wall of the cylinder 421, the extended end of the driven rod 425 is fixedly connected with the lifting rod 490, the sliding block 424 is slidably connected in the curve groove 423 of the outer wall of the cylindrical cam 422, when the cylindrical cam 422 rotates, the shape of the curve groove 423 forces the sliding block 424 to slide in the groove, the sliding block 424 is connected with the lifting rod 490 through the driven rod 425, thereby driving the lifting rod 490 to make axial reciprocating motion; the lifting rod 490 is fixedly connected with a lifting seat 480, the lifting seat 480 is connected with a support rod 460 through a transmission rod 470, the support rod 460 is rotatably connected with a connecting seat 450 at the top end of the stirring shaft 330, when the lifting rod 490 makes axial reciprocating motion, the lifting seat 480 is driven to move up and down, the support rod 460 is driven to rotate around the connecting seat 450 through the transmission rod 470, thereby enabling the air bag 50 with the inverted conical structure to realize contraction and expansion.

[0027] The top of the tank body 10 is further provided with a gas pressure balancing assembly for balancing the gas pressure in the inner cavity of the air bag 50 and the inner cavity of the tank body 10. Referring to Figure 1 , Figure 2 and Figure 3 , the gas pressure balancing assembly comprises a pressure sensor installed in the inner side wall of the tank body 10 and the inner cavity of the air bag 50, the top of the air bag 50 is further provided with an electrically controlled air inlet valve 510 and an electrically controlled air outlet valve 520, the electrically controlled air inlet valve 510 is further provided with a compensation pipe 530 extending outwardly through the tank cover 110, the extended end of the compensation pipe 530 is connected with a gas pressure compensation gas tank 540, a compensation valve 550 is arranged at the joint of the gas pressure compensation gas tank 540 and the compensation pipe 530, the gas pressure in the gas pressure compensation gas tank 540 is greater than the gas pressure in the inner cavity of the tank body 10, the inner side wall of the tank body 10 and the inner cavity of the air bag 50 are both provided with a pressure sensor, when the air bag 50 expands or contracts, the internal gas pressure will change, if the gas pressure in the inner cavity of the air bag 50 is lower than the gas pressure in the inner cavity of the tank body 10, after the pressure sensor detects the pressure difference, the controller controls the electrically controlled air inlet valve 510 to open, the gas in the gas pressure compensation gas tank 540 enters the air bag 50 through the compensation pipe 530 to balance the gas pressure; if the gas pressure in the inner cavity of the air bag 50 is higher than the gas pressure in the inner cavity of the tank body 10, the electrically controlled air outlet valve 520 is opened, the gas in the air bag 50 is discharged to maintain the gas pressure balance, to ensure that the air bag 50 deforms within a normal pressure range, and to avoid damage to the air bag or affect the substrate diffusion effect due to unbalanced gas pressure.

[0028] Reference Figure 3 and Figure 4 The lifting rod 490 is fixedly connected to the outer wall of the rod end near the lifting seat 480 with a horizontally arranged connecting rod 491, and the end of the connecting rod 491 away from the lifting rod 490 is fixedly connected to the flexible cleaning ring 492 which contacts the inner wall of the tank body 10. The lifting rod 490 is fixedly connected to the outer wall of the rod end near the lifting seat 480 with the connecting rod 491, and the flexible cleaning ring 492 at the other end contacts the inner wall of the tank body 10 as the lifting rod 490 moves. During the contraction and expansion movement of the airbag 50, the flexible cleaning ring 492 continuously scrapes the inner wall of the tank body 10, promptly cleaning the substrate attached to the tank wall and reducing the amount of substrate attachment on the inner wall of the tank body. In this process, the "pumping effect" generated by the reciprocating lifting pushes the substrate outward when expanding and sucks the substrate inward when contracting, which can disturb the stagnant layer near the wall of the reactor and further reduce the deposition of the substrate on the wall.

[0029] Reference Figure 2 、 Figure 3 、 Figure 4 and Figure 5 The first driving assembly 310 includes a base 311 mounted at the bottom of the tank 10 and having a hollow inner cavity, and a first servo motor 312 is embedded in the inner cavity of the base 311; The first internal and external magnetic coupling assembly 320 includes a first outer magnetic ring 321 fixedly connected to the output end of the first servo motor 312. A first inner magnetic ring 322, which is arranged opposite to the first outer magnetic ring 321, is fixedly connected to the outer side wall of the stirring shaft 330 near the bottom end. The first servo motor 312 is embedded in the inner cavity of the base 311. When started, it drives the first outer magnetic ring 321 to rotate. The first outer magnetic ring 321 and the first inner magnetic ring 322 on the stirring shaft 330 are magnetically coupled to achieve contactless transmission, driving the stirring shaft 330 to rotate within the tank body. This magnetic coupling method avoids the leakage risk of traditional mechanical seals and ensures the sterile environment and cleanliness inside the tank body. The suction-type lifting assembly includes a suction-type paddle 360 ​​fixedly mounted on the outer wall of the first inner magnetic ring 322. When the stirring shaft 330 rotates, the suction-type paddle 360 ​​fixedly mounted on the outer wall of the first inner magnetic ring 322 rotates accordingly. The special structure of the suction-type paddle 360 ​​enables it to form a certain negative pressure at the bottom of the stirring shaft 330 during rotation, sucking in the substrate at the bottom of the tank and lifting it upward, thereby promoting the circulation of the substrate. The flow guide assembly 350 comprises a flow guide ring 351 fixedly installed on the middle section of the shaft wall of the stirring shaft 330 through a support rod, the inner side wall of the flow guide ring 351 is fixedly connected with flow guide plates 352 which are uniformly distributed and obliquely arranged, the middle section of the stirring shaft 330 is provided with a flow guide groove 353 which is in communication with the inner cavity of the stirring shaft and is opposite to the flow guide plates 352, and the flow guide ring 351 of the middle section of the stirring shaft 330 is fixed on the shaft wall through a support rod, the flow guide plates 352 are fixed on the inner side wall of the flow guide ring 351 and are obliquely arranged, when the stirring shaft 330 rotates, the flow guide plates 352 guide the fluid to generate axial and radial mixed flow, further enhancing the mixing effect of the fluid and optimizing the distribution of the substrate in the tank; The bubble breaking assembly 340 comprises disc-shaped stirring sub 341 which is equidistantly installed on the outer wall of the upper section of the stirring shaft 330, the outer side wall of the stirring sub 341 is provided with uniformly distributed flat sawtooth pieces 342, and the length of the flat sawtooth pieces 342 decreases in the vertical direction in turn, the disc-shaped stirring sub 341 which is equidistantly installed on the outer wall of the upper section of the stirring shaft 330 is provided with flat sawtooth pieces 342 on the outer side wall, which rotate at high speed when the stirring shaft 330 rotates, the bubbles in the rising process meet these high-speed rotating flat sawtooth pieces 342 and are cut and broken, since the length of the flat sawtooth pieces 342 decreases in the vertical direction in turn, bubbles of different sizes will be broken by sawtooth pieces of different lengths in the rising process, effectively reducing the amount of substrate carried by the bubbles in the rising process and reducing the substrate loss rate.

[0030] Referring to Figure 2 and Figure 9The gas distributor 20 includes a first annular air pipe 210 installed on the inner wall of the bottom of the tank body 10, a second annular air pipe 220 is arranged above the first annular air pipe 210, and the second annular air pipe 220 is provided with gas injection holes 230 evenly distributed along the circumference, a one-way valve 240 is connected between the first annular air pipe 210 and the second annular air pipe 220, and the tank cover 110 is provided with an air inlet pipe 250 extending toward the bottom of the tank body 10, and the air inlet of the air inlet pipe 250 is provided with an electromagnetic valve, and one end of the air inlet pipe 250 placed in the tank body 10 is connected to the first annular air pipe 210, and the first annular air pipe 210 and the second annular air pipe 220 are sleeved on the outside of the spoiler column 120. After the electromagnetic valve on the air inlet pipe 250 is opened, the gas enters the air inlet pipe 250 from the tank cover 110 and flows into the first annular air pipe 210. A one-way valve 240 is installed in the middle, and the gas can only flow from the first annular air pipe 210 to the second annular air pipe 220. The gas injection holes 230 evenly distributed along the circumference of the second annular air pipe 220 disperse the gas into the bottom area of ​​the tank body 10. During the rising process, the dispersed gas initially drives the reaction raw materials to mix, breaks the static distribution of the raw materials in the tank, and makes the substrate initially contact with the gas environment required for fermentation, laying the foundation for subsequent reactions. At the same time, the gas distributor is sleeved on the outside of the spoiler column 120, and the spoiler column 120 further interferes with the rising airflow, so that the gas and raw materials are mixed more evenly. In the initial stage of ventilation, pay close attention to the gas distribution. It can be judged by observing the degree of churning of the liquid in the tank and the uniformity of the rising bubbles. If the gas distribution is found to be uneven, such as too many or too few local bubbles, check whether the gas distributor 20 is blocked or damaged, and clean or repair it in time.

[0031] The system further includes a controller, which is connected to the magnetic coupling stirring mechanism 30 , the adjustable inverted cone-shaped dynamic diffusion component 40 , the air pressure balance component and the gas distributor 20 by signal.

[0032] Reference Figure 1 and Figure 2The bottom center of the tank body 10 is embedded with a low-frequency ultrasonic vibrator for destroying the adhesion between the substrate and the inner wall of the bottom of the tank body 10. When the low-frequency ultrasonic vibrator embedded at the center of the bottom of the tank body 10 works, it emits low-frequency ultrasonic waves, which act on the substrate at the bottom of the tank body, destroying the adhesion between the substrate and the inner wall of the bottom of the tank body 10, making it easier for the substrate deposited at the bottom to be stirred and driven by the fluid movement, thereby reducing the accumulation of the substrate at the bottom. The bottom of the tank body 10 adopts a conical structure, the outer wall of the tank body 10 is provided with a water-cooling jacket 140, and the tank cover 110 is provided with a pressure gauge, a pressure relief valve, a temperature control valve, and a pressure relief valve. A thermometer and a pH meter are provided. A discharge port is provided on the outer wall of the tank body 10 near the bottom, a dissolved oxygen monitoring sensor is provided on the inner wall of the tank body 10, and a pressure gauge monitors the pressure in the tank in real time. When the pressure in the tank exceeds the set threshold, the pressure relief valve automatically opens to release part of the gas in the tank to prevent the pressure in the tank from being too high and ensure the safe operation of the reactor. A water-cooling jacket 140 is installed on the outer wall of the tank body 10. Through the circulating cooling medium such as water, the heat generated during the fermentation process is taken away, and the temperature in the tank is accurately controlled within a suitable range, providing a stable temperature environment for bile salt hydrolase fermentation.

[0033] According to the production process requirements, accurately prepare the reaction raw materials to ensure that the concentration, purity and other indicators of the raw materials meet the requirements. Perform necessary pretreatment on the raw materials, such as filtering to remove impurities and adjusting the pH value. For example, adjust the pH value of the raw materials to 6.5-7.5 to meet the suitable environment for bile salt hydrolase fermentation. Slowly add the prepared raw materials into the tank through the feed port. During the addition process, pay attention to the changes in the liquid level in the tank to avoid excessive addition that may cause the raw materials to overflow or generate a large amount of foam. After the solenoid valve on the air inlet pipe 250 is opened, the gas enters the air inlet pipe 250 from the tank cover 110 and flows into the first annular air pipe 210. Since a one-way valve 240 is installed between the first annular air pipe 210 and the second annular air pipe 220, the gas can only flow from the first annular air pipe 210 to the second annular air pipe 220. The gas injection holes 230 uniformly distributed along the circumference of the second annular air pipe 220 disperse the gas into the bottom area of ​​the tank body 10. The dispersed gas initially drives the reaction raw materials to mix during the rising process, breaking the raw materials in the tank. Static distribution allows the substrate to initially contact the gas environment required for fermentation, laying the foundation for subsequent reactions. At the same time, the gas distributor is placed on the outside of the spoiler column 120, which further interferes with the rising airflow, making the gas and raw materials mix more evenly. In the early stage of ventilation, pay close attention to the gas distribution. It can be judged by observing the degree of churning of the liquid in the tank and the uniformity of the rising bubbles. If the gas distribution is found to be uneven, such as too many or too few bubbles in some areas, check whether the gas distributor 20 is blocked or damaged, and clean or repair it in time.

[0034] The fermentation process and the dissolved oxygen concentration in the tank are monitored by a dissolved oxygen monitoring sensor installed in the tank, and the aeration amount is adjusted in time, for example, the aeration amount is appropriately increased when the dissolved oxygen concentration is lower than the set lower limit; the aeration amount is appropriately reduced when the dissolved oxygen concentration is higher than the set upper limit; The first servo motor 312 is embedded in the inner cavity of the base 311, and drives the first outer magnetic ring 321 to rotate after being started. The first outer magnetic ring 321 and the first inner magnetic ring 322 on the stirring shaft 330 are coupled by magnetic force, realizing non-contact transmission and driving the stirring shaft 330 to rotate in the tank body. This magnetic coupling mode avoids the leakage risk of traditional mechanical seals, ensuring the sterile environment and cleanliness inside the tank body. When the stirring shaft 330 rotates, the suction paddle 360 fixedly installed on the outer wall of the first inner magnetic ring 322 rotates. The special structure of the suction paddle 360 can form a certain negative pressure at the bottom end of the stirring shaft 330 during rotation, and the substrate at the bottom of the tank is sucked and lifted upwards, promoting the circulation of the substrate. The flow guide ring 351 in the middle section of the stirring shaft 330 is fixed to the shaft wall through a support rod, and the flow guide plate 352 is fixed to the inner side wall of the flow guide ring 351 and is inclined. When the stirring shaft 330 rotates, the flow guide plate 352 guides the fluid to produce axial and radial mixing flow, further enhancing the mixing effect of the fluid and optimizing the distribution of the substrate in the tank. The disc-shaped stirring sub 341 is installed at equal distances on the outer wall of the upper section of the stirring shaft 330, and the flat sawtooth blade 342 is arranged on the outer side wall. When the stirring shaft 330 rotates, the flat sawtooth blade 342 rotates at high speed. The bubbles in the rising process meet these high-speed rotating flat sawtooth blades 342 and are cut and broken. Since the length of the flat sawtooth blade 342 decreases along the vertical direction, different sizes of bubbles will be broken by different lengths of sawtooth blades in the rising process, effectively reducing the amount of substrate carried by the bubbles in the rising process and reducing the substrate loss rate. The second servo motor 410 is installed on the top of the tank cover 110, and drives the second outer magnetic ring 441 to rotate after being started. The second outer magnetic ring 441 and the second inner magnetic ring 442 at the top end of the rotating rod 430 are coupled by magnetic force, so that the rotating rod 430 rotates on the mounting bracket 111, and the cylindrical cam 422 connected to the bottom end of the rotating rod 430 rotates. The slider 424 is slidably connected in the curved groove 423 on the outer wall of the cylindrical cam 422. When the cylindrical cam 422 rotates, the shape of the curved groove 423 forces the slider 424 to slide in the groove. The slider 424 is connected to the lifting rod 490 through the driven rod 425, thereby driving the lifting rod 490 to perform axial reciprocating motion; the lifting rod 490 is fixedly connected to the lifting seat 480, and the lifting seat 480 is connected to the support rod 460 through the transmission rod 470. The support rod 460 is rotatably connected to the connecting seat 450 at the top of the stirring shaft 330. When the lifting rod 490 performs axial reciprocating motion, it drives the lifting seat 480 to move up and down, and the support rod 460 is rotated around the connecting seat 450 through the transmission rod 470, thereby causing the airbag 50 with an inverted cone structure to contract and expand; When the airbag 50 expands, it diffuses the rising substrate along the cone surface to the edge of the tank body 10, increasing the diffusion range of the substrate; when it contracts, it facilitates the substrate to gather in the central area to prepare for the next diffusion; the airbag 50 folds and the ribs are retracted to reduce the liquid flow resistance, making it easier for the substrate to gather in the central area along with the rising flow. When the airbag 50 expands, the angle between the support rod 460 and the transmission rod 470 becomes smaller, and the "umbrella surface" of the umbrella frame mechanism formed by it becomes larger, pushing the substrate to spread to the edge of the tank body 10. When the airbag 50 contracts, the angle between the support rod 460 and the transmission rod 470 becomes larger, and the umbrella frame is retracted, reducing the resistance to liquid flow, so that the substrate can gather in the central area with the rising flow. The lifting rod 490 is fixed to the outer wall of the rod end close to the lifting seat 480, and the flexible cleaning ring 492 at the other end thereof contacts the inner wall of the tank body 10 as the lifting rod 490 moves. During the contraction and expansion of the airbag 50, the flexible cleaning ring 492 continuously scrapes the inner wall of the tank body 10, promptly cleaning the substrate attached to the tank wall and reducing the amount of substrate attached to the inner wall of the tank. In this process, the "pumping effect" generated by the reciprocating lifting pushes the substrate outward when expanding and sucks the substrate inward when contracting, which can disturb the stagnant layer near the wall of the reactor and further reduce the deposition of the substrate on the wall. Pressure sensors are installed on the inner wall of the tank body 10 and the inner cavity of the airbag 50. When the airbag 50 expands or contracts, the internal air pressure will change. If the air pressure in the airbag 50 is lower than the air pressure in the tank body 10, the pressure sensor detects the pressure difference, and the controller controls the electronically controlled air inlet valve 510 to open, and the gas in the pressure compensation gas tank 540 enters the airbag 50 through the compensation tube 530 to balance the air pressure; if the air pressure in the airbag 50 is higher than the air pressure in the tank body 10, the electronically controlled exhaust valve 520 opens, and the gas in the airbag 50 is discharged to maintain the air pressure balance, ensuring that the airbag 50 deforms within the normal pressure range, avoiding damage to the airbag or affecting the substrate diffusion effect due to air pressure imbalance; The controller monitors the temperature, pH, dissolved oxygen, substrate concentration and other parameters during the fermentation process in real time, and fine-tunes the operating parameters of each component as needed to ensure a stable and efficient fermentation process.

[0035] The above description is only a preferred specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any technician familiar with the technical field, within the technical scope disclosed by the present invention, who makes equivalent replacements or changes based on the technical solution and inventive concept of the present invention, should be covered by the scope of protection of the present invention.

Claims

1. An airlift reactor for continuous fermentation production of bile salt hydrolase, characterized in that: include: A tank body (10), wherein a tank cover (110) is provided on the top of the tank body (10), a feed port is provided on the tank cover (110), and evenly distributed spoiler columns (120) are provided at the center of the bottom inner wall of the tank body (10), and a positioning ring (130) is fixedly connected to the top of the spoiler column (120); A gas distributor (20), the gas distributor (20) being installed at the bottom of the tank body (10) and used to introduce dispersed gas into the tank body (10) to accelerate mixing of the reaction raw materials; A magnetic coupling stirring mechanism (30), the magnetic coupling stirring mechanism (30) comprising a first driving assembly (310) mounted on the bottom of the tank body (10), the output end of the first driving assembly (310) being mounted with a first internal and external magnetic coupling assembly (320) acting on the inner cavity of the tank body (10), the working end of the first internal and external magnetic coupling assembly (320) being placed in the inner cavity of the tank body (10) being connected with a stirring shaft (330) having a clearance fit with the inner wall of the positioning ring (130), the bottom end of the stirring shaft (330) being open and the inner cavity of the middle and lower sections being hollow, and the outer wall of the stirring shaft (330) being provided with a bubble breaking assembly (340), a flow guide assembly (350) and a suction-type rising assembly in sequence along the vertical direction; An adjustable inverted cone-shaped dynamic diffusion component (40) is installed between the tank cover (110) and the top end of the stirring shaft (330) and is used to diffuse the rising substrate along the cone surface to the edge of the tank body (10) to increase the diffusion range of the substrate.

2. The airlift reactor for continuous fermentation production of bile salt hydrolase according to claim 1, wherein The adjustable inverted cone-shaped dynamic diffusion assembly (40) includes a second drive assembly mounted at the center of the top of the tank cover (110); a mounting frame (111) is fixedly connected to the inner wall of the top of the tank body (10); a rotating rod (430) is rotatably connected to the mounting frame (111); a second internal and external magnetic coupling assembly (440) is connected between the output end of the second drive assembly and the end of the rotating rod (430) close to the tank cover (110); a connecting seat (450) is rotatably connected to the top of the stirring shaft (330); and support rods (460) are evenly distributed and tilted and rotatably connected to the outer wall of the connecting seat (450). The support rods (460) One end away from the connecting seat (450) is rotatably connected to a transmission rod (470), and one end of the transmission rod (470) away from the support rod (460) is rotatably connected to a lifting seat (480). The top of the lifting seat (480) is fixedly connected to a lifting rod (490), and a cylindrical cam assembly (420) is connected between the top of the lifting rod (490) and the bottom end of the rotating rod (430). An air bag (50) with an inverted cone structure is fixedly connected to the outer wall of the support rod (460), and the air bag (50) is placed in the umbrella frame space formed by the support rod (460) and the transmission rod (470), and the air bag (50) and the transmission rod (470) are clearance-matched. The second driving assembly comprises a second servo motor (410) mounted on the top of the tank cover (110) via a bracket; The second inner and outer magnetic coupling assembly (440) comprises a second outer magnetic ring (441) fixedly mounted on the output end of the second servo motor (410), and the top end of the rotating rod (430) is fixedly connected to a second inner magnetic ring (442) arranged opposite to the second outer magnetic ring (441).

3. The airlift reactor for continuous fermentation production of bile salt hydrolase according to claim 2, wherein: The cylindrical cam assembly (420) includes a cylinder (421) fixedly connected to the bottom of the mounting frame (111) through a support rod, the bottom end of the rotating rod (430) is placed through the outer wall of the cylinder (421) and extends into the inner cavity of the cylinder (421), the extended end of the rotating rod (430) is fixedly connected to the cylindrical cam (422), the outer wall of the cylindrical cam (422) is provided with a curved groove (423) connected end to end, a slider (424) is slidably connected in the curved groove (423), the outer wall of the slider (424) is connected to a driven rod (425), the top end of the lifting rod (490) passes through the outer wall of the cylinder (421) and extends into the inner cavity of the cylinder (421), and the driven rod (425) is fixedly connected to the extended end of the lifting rod (490).

4. The airlift reactor for continuous fermentation production of bile salt hydrolase according to claim 3, characterized in that: The top of the tank body (10) is also provided with an air pressure balancing component for balancing the air pressure in the inner cavity of the air bag (50) and the inner cavity of the tank body (10); The air pressure balancing component includes a pressure sensor installed on the inner wall of the tank body (10) and in the inner cavity of the airbag (50). The top of the airbag (50) is also provided with an electrically controlled air intake valve (510) and an electrically controlled air exhaust valve (520). The electrically controlled air intake valve (510) is also provided with a compensation tube (530). The compensation tube (530) passes through the tank cover (110) and extends outward. The extended end of the compensation tube (530) is connected to an air pressure compensation gas tank (540). A compensation valve (550) is provided at the intersection of the air pressure compensation gas tank (540) and the compensation tube (530). The air pressure in the air pressure compensation gas tank (540) is greater than the air pressure in the inner cavity of the tank body (10).

5. The airlift reactor for continuous fermentation production of bile salt hydrolase according to claim 4, characterized in that: The outer wall of the rod end of the lifting rod (490) close to the lifting seat (480) is fixedly connected to a horizontally arranged connecting rod (491), and one end of the connecting rod (491) away from the lifting rod (490) is fixedly connected to a flexible cleaning ring (492) that contacts the inner wall of the tank body (10).

6. The airlift reactor for continuous fermentation production of bile salt hydrolase according to claim 5, characterized in that: The first driving assembly (310) comprises a base (311) mounted on the bottom of the tank body (10) and having a hollow inner cavity, wherein a first servo motor (312) is embedded in the inner cavity of the base (311); The first internal and external magnetic coupling assembly (320) comprises a first outer magnetic ring (321) fixedly connected to the output end of the first servo motor (312); and a first inner magnetic ring (322) arranged opposite to the first outer magnetic ring (321) is fixedly connected to the outer side wall of the stirring shaft (330) near the bottom end. The suction-type lifting component comprises a suction-type blade (360) fixedly mounted on the outer wall of the first inner magnetic ring (322); The flow guide assembly (350) comprises a flow guide ring (351) fixedly mounted on the middle shaft wall of the stirring shaft (330) via a support rod, the inner side wall of the flow guide ring (351) is fixedly connected with a flow guide plate (352) that is evenly distributed and tilted, and the middle section of the stirring shaft (330) is provided with a flow guide groove (353) that is in communication with the inner cavity of the stirring shaft, and the flow guide groove (353) is directly opposite to the flow guide plate (352); The bubble breaking assembly (340) comprises a stirrer (341) of a disc-shaped structure mounted at equal distances on the outer wall of the upper section of the stirring shaft (330); the outer wall of the stirrer (341) is provided with evenly distributed straight sawtooth pieces (342), and the lengths of the straight sawtooth pieces (342) decrease sequentially along the vertical direction.

7. The airlift reactor for continuous fermentation production of bile salt hydrolase according to claim 6, characterized in that: The gas distributor (20) includes a first annular air pipe (210) installed on the inner wall of the bottom of the tank body (10), a second annular air pipe (220) is arranged above the first annular air pipe (210), and the second annular air pipe (220) is provided with gas injection holes (230) evenly distributed along the circumference, and a one-way valve (240) is connected between the first annular air pipe (210) and the second annular air pipe (220). The tank cover (110) is provided with an air inlet pipe (250) extending toward the bottom of the tank body (10), and the air inlet of the air inlet pipe (250) is provided with a solenoid valve. One end of the air inlet pipe (250) placed in the tank body (10) is connected to the first annular air pipe (210), and the first annular air pipe (210) and the second annular air pipe (220) are sleeved on the outside of the spoiler column (120).

8. The airlift reactor for continuous fermentation production of bile salt hydrolase according to claim 7, characterized in that: It also includes a controller, which is connected to the magnetic coupling stirring mechanism (30), the adjustable inverted cone-shaped dynamic diffusion component (40), the air pressure balance component and the gas distributor (20) by signal.

9. The airlift reactor for continuous fermentation production of bile salt hydrolase according to claim 1, characterized in that: A low-frequency ultrasonic vibrator for destroying the adhesion between the substrate and the inner wall of the bottom of the tank body (10) is embedded at the center of the bottom of the tank body (10), and the bottom of the tank body (10) adopts a conical surface structure.

10. The airlift reactor for continuous fermentation production of bile salt hydrolase according to claim 1, characterized in that: The outer wall of the tank body (10) is provided with a water-cooling jacket (140), the tank cover (110) is provided with a pressure gauge, a pressure relief valve, a thermometer and a pH meter, the outer wall of the tank body (10) near the bottom is provided with a discharge port, and the inner wall of the tank body (10) is provided with a dissolved oxygen monitoring sensor.

Images