Special liquid multi-vitamin compound nutrient for livestock and poultry as well as preparation method and application of special liquid multi-vitamin compound nutrient

Abstract

The invention discloses a special liquid multi-vitamin compound nutrient for livestock and poultry as well as a preparation method and application of the special liquid multi-vitamin compound nutrient. The method comprises the following steps: (1) preparing an oil phase containing fat-soluble vitamins, an emulsifier and fish oil; (2) preparing a water phase containing water-soluble vitamins, amino acids and a traditional Chinese medicine preparation, wherein the traditional Chinese medicine preparation is traditional Chinese medicine preparation modified Sijunzi decoction; (3) adding the water phase into the oil phase for emulsification reaction in batches, wherein the mass ratio of the oil phase to the water phase is 1: (4-6); (4) performing high-pressure homogenization treatment on the emulsion to obtain a nanoscale emulsion, wherein the particle size of the emulsion is less than 30nm; and (5) filtering, sterilizing and packaging. The nano-emulsion is prepared by optimizing the oil-water phase ratio and a fractional emulsification process and combining a high-pressure homogenization technology, the stability and bioavailability of fat-soluble vitamins are remarkably improved, meanwhile, the added traditional Chinese medicine preparation, namely the modified Sijunzi decoction, effectively enhances the immune regulation function of livestock, and the product is good in stabilizing effect and free of toxic and side effects. The method can be widely applied to the livestock breeding field.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of livestock feed, more particularly, to a liquid multi-vitamin compound for livestock and its preparation method and application. BACKGROUND

[0002] Vitamins, as essential nutrients for animal bodies, play an irreplaceable role in the growth and development, immune regulation and metabolic processes of livestock. However, traditional powder-type vitamins have obvious limitations in practical application. First, their bioavailability is generally low, only 25-30%, which not only wastes resources but also directly affects the production performance of livestock. Second, the powder is easily destroyed by high temperature during feed processing, and has problems such as moisture absorption and caking, further reducing the stability of the effective ingredients.

[0003] With the development of intensive farming, liquid preparations have gradually become the main research and development direction of vitamin supplements. However, existing liquid vitamin products still face the following problems: First, the stability problem, especially the fat-soluble vitamins (such as A, D, E) are easily affected by light, heat, oxygen and other factors, and are prone to oxidative degradation, resulting in a large loss of activity during storage. Second, the absorption and utilization rate of conventional preparations needs to be improved in young livestock or under stress. In addition, there are also problems such as uniformity of feed mixing and blockage of the drinking water system in practical application.

[0004] Chinese patent application document (202110078732.2) discloses a preparation method for a compound liquid vitamin for livestock, comprising the following steps: weighing appropriate amounts of 2.6 million IU of vitamin A oil, 25 million IU of vitamin D3 oil and 92.5% vitamin E oil, and sequentially placing them into an ultrasonic treatment tank, adding an appropriate amount of Tween 80, and then performing ultrasonic treatment at room temperature to obtain nanoemulsion; weighing an appropriate amount of water-soluble riboflavin phosphatidyl acid sodium, then adding an appropriate amount of purified water and dissolving thoroughly for standby use. Although the solution of the document has better clarity and ensures the quality of the product, it lacks targeted design for the digestive system of livestock and has limited improvement effect on sub-health state.

[0005] It is worth noting that the active ingredients in traditional Chinese medicine preparations have been proven to have significant immune regulation effects. How to introduce active ingredients of Chinese medicine with immune regulation function into compound vitamin preparations to form a synergistic effect between Chinese medicine preparations and vitamins, and to develop a livestock-specific nutrient preparation with good stability, high bioavailability and immune regulation function has important research value and application prospect. SUMMARY

[0006] Therefore, it is necessary to provide a liquid multi-vitamin compound for livestock and its preparation method and application in view of the above technical problems.

[0007] To solve the above technical problems, the first aspect of the present application provides a preparation method of liquid multi-vitamin compound nutrients for livestock and poultry, comprising the following steps:

[0008] (1) preparing an oil phase containing fat-soluble vitamins, emulsifiers and fish oil;

[0009] (2) preparing an aqueous phase containing water-soluble vitamins, amino acids and traditional Chinese medicine preparation, wherein the traditional Chinese medicine preparation is modified Sijunzi decoction;

[0010] (3) adding the aqueous phase into the oil phase in several portions for emulsification reaction, and the mass ratio of the oil phase to the aqueous phase is 1:4-6;

[0011] (4) performing high-pressure homogenization treatment on the emulsion to obtain a nano-emulsion, wherein the particle size of the emulsion is less than 30 nm;

[0012] (5) performing sterilization and packaging after filtration.

[0013] Further, the oil phase contains the following components by mass fraction: vitamin A 2-4, vitamin D3 0.1-0.3, vitamin E 8-15, tea polyphenol 0.6-1, glycerol 10-20, fish oil 3-6, potassium hydroxide 1-3, and Tween-80 50-75.3.

[0014] Further, the aqueous phase contains the following components by mass fraction: vitamin B1 2-3, vitamin B2 0.5-0.8, vitamin B6 0.5-0.8, biotin-vitamin B7 1-2, folic acid-vitamin B9 1-2, vitamin C 10-15, lysine 1.5-3, methionine 3-4, taurine 12-18, traditional Chinese medicine preparation 2-3, potassium hydroxide 0.1-0.3, and sterile deionized water 40-70. 11 1-2, vitamin B 12 1-2, vitamin C 10-15, lysine 1.5-3, methionine 3-4, taurine 12-18, traditional Chinese medicine preparation 2-3, potassium hydroxide 0.1-0.3, and sterile deionized water 40-70.

[0015] Further, the aqueous phase is added into the oil phase in five portions in the emulsification reaction step.

[0016] Further, the modified Sijunzi decoction comprises Dangshen polysaccharide extract, Baizhu extract, Fuling polysaccharide extract, Gancao extract, Buguzhi extract, Cogongrass extract, Duzhong extract and Angelica sinensis polysaccharide extract.

[0017] Further, the modified Sijunzi decoction contains the following components by mass fraction: Dangshen polysaccharide extract 15-20, Baizhu extract 15-20, Fuling polysaccharide 5-10, Gancao extract 5-10, Buguzhi extract 15-20, Cogongrass extract 15-20, Duzhong extract 15-20, and Angelica sinensis polysaccharide extract 15-20.

[0018] Furthermore, the high-pressure homogenization treatment is specifically a cyclic treatment at 100-120 MPa and 60-68° C. for 3-5 times.

[0019] Furthermore, the emulsification reaction is carried out at 60-68° C., and the pH is adjusted to 3.4-6.3; and the filtration uses a 0.11-0.22 μm filter membrane.

[0020] The second aspect of the present invention provides a liquid multi-dimensional composite nutrient for livestock and poultry prepared by any of the above methods, which comprises a nanoemulsion with a particle size of 20 to 30 nm.

[0021] The third aspect of the present invention proposes the application of liquid multi-dimensional composite nutrients for livestock and poultry, including the following application methods:

[0022] (1) Application in pig farming: Add to the pig drinking water system at a ratio of 1 kg / ton of water, once a day;

[0023] (2) Application in poultry farming: Add to the drinking water system of laying hens at a ratio of 1 ml / L water, once a day;

[0024] (3) The application method is suitable for nutritional supplementation throughout the entire growth cycle of livestock and poultry.

[0025] Compared with the prior art, the present invention has the following beneficial effects:

[0026] The present invention provides a liquid multi-dimensional composite nutrient for livestock and poultry, and its preparation method and application. The preparation method significantly improves the product stability and the bioavailability of nutrients by scientifically regulating the ratio of oil phase to water phase and adopting a graded emulsification process, combined with high-pressure homogenization treatment. The particle size of the nanoemulsion prepared by the method provided by the present invention is controlled within the range of 20 to 30 nm and is evenly distributed, so that fat-soluble vitamins can be stably dispersed in the aqueous phase system for a long time. In particular, the present invention achieves multiple synergies by adding a specific ratio of traditional Chinese medicine preparations to the flavored Four Gentlemen Decoction. Codonopsis pilosula and Atractylodes macrocephala promote nutrient absorption, Cistanche deserticola and Eucommia ulmoides regulate reproductive hormones, Psoralea corylifolia and Angelica sinensis improve microcirculation and enhance the vitality of livestock and poultry, and Licorice and Poria cocos resist stress and strengthen immunity. The multi-target synergy significantly improves the reproduction and fattening performance of livestock and poultry, effectively enhances the body's immune regulation function, improves the intestinal health of livestock and poultry, and achieves a comprehensive improvement in production performance.

[0027] Testing of the liquid multi-dimensional composite nutrient for livestock and poultry prepared by this invention shows that after 60 days of storage at 45°C, the vitamin activity retention rate exceeds 99.5%. Animal experiments also confirm that it can significantly improve the absorption and utilization rate of nutrients. The preparation method proposed in this invention is simple and efficient, and the product has stable performance. It can be widely used in the livestock and poultry breeding industry to improve animal production performance and health. DETAILED DESCRIPTION

[0028] In order to make the person skilled in the art better understand the application scheme, the technical solutions in the embodiments of the application will be clearly and completely described below. Obviously, the described embodiments are only a part of the embodiments of the application, not all. Based on the embodiments in the application, all other embodiments obtained by those skilled in the art without creative labor should belong to the protection scope of the application.

[0029] In order to make the person skilled in the art better understand the application scheme, the technical solutions in the embodiments of the application will be clearly and completely described below.

[0030] Embodiment 1

[0031] The embodiment provides a preparation method of traditional Chinese medicine preparation Jiawei Sijunzi Decoction and Sijunzi Decoction, which comprises the following steps:

[0032] S1, extract the radix ginseng polysaccharide extract, atractylodes extract, poria cocos polysaccharide extract, liquorice extract, psoralea extract, cistanche extract, eucommia extract and angelica polysaccharide extract according to the following steps respectively:

[0033] 1, the extraction method of radix ginseng polysaccharide extract, comprising the following steps:

[0034] 1.1 pretreatment of raw materials: put radix ginseng in the air blast drying oven, and bake at 60 DEG C to constant weight, and then crush into fine powder with a pulverizer;

[0035] 1.2 defatting treatment: defatting with petroleum ether for 3 times (2 hours each time);

[0036] 1.3 water extraction treatment: add distilled water according to the ratio of 1:20, soak at room temperature for 12 hours, centrifuge at 4000 r / min for 15 minutes, and collect the supernatant;

[0037] 1.4 initial concentration treatment: filter the filtrate and concentrate under reduced pressure to 1 / 4 of the original volume;

[0038] 1.5 first alcohol precipitation: add 3 times the volume of 95% ethanol, and stand in the refrigerator at 4 DEG C for 12 hours, centrifuge at 4000 r / min for 15 minutes, and collect the precipitate;

[0039] 1.6 deproteinization purification: redissolve the precipitate with sterile deionized water, add Sevage reagent (chloroform: n-butanol = 4:1), shake for 30 minutes, and then centrifuge at 4000 r / min for 15 minutes;

[0040] 1.7 second alcohol precipitation: repeat step 1.5 to carry out second alcohol precipitation;

[0041] 1.8 Final treatment: the precipitate is washed with anhydrous ether for 3 times, and dried under vacuum to obtain the extract of radix codonopsis polysaccharide

[0042] 2. The extraction method of the extract of Atractylodes macrocephala Koidz, comprising the following steps:

[0043] 2.1 Raw material pretreatment: 1 part of Atractylodes macrocephala Koidz is dried in a 70℃ oven until the water content is less than or equal to 5%, and is ground into fine powder. The Atractylodes macrocephala Koidz powder is irradiated with an electron beam at 10 kGy (sterilization and auxiliary wall breaking);

[0044] 2.2 Ethanol extraction (mixture A): a 70% ethanol solution (10 parts of Atractylodes macrocephala Koidz) is prepared, the pH is adjusted to 5 with citric acid, the Atractylodes macrocephala Koidz powder is added, and ultrasonic extraction is performed at 40℃ for 5 min, and stirring is performed at 50℃ for 1 h. The first filtrate (mixture A) is obtained by filtration, and the first residue is obtained by rotary evaporation;

[0045] 2.3 Organic solvent extraction (mixture B): the first residue is added to 8 parts of an organic solvent (ethyl acetate or acetone), ultrasonic extraction is performed at 30℃ for 5 min, and stirring is performed at 40℃ for 30 min. The second filtrate (mixture B) is obtained by filtration, and the second residue is obtained by rotary evaporation;

[0046] 2.4 Enzymatic hydrolysis-water extraction (mixture C): the second residue is added to 50 parts of deionized water, the pH is adjusted to 4.5 with acetic acid, 0.0001 parts of pectinase and 0.0001 parts of cellulase are added, and enzymatic hydrolysis is performed at 45℃ for 10 min. Then, high-temperature cooking is performed at 3 MPa and 150℃ for 10 min, and the third filtrate is obtained by filtration after cooling to 30℃. The mixture C is obtained by concentrating under reduced pressure.

[0047] 2.5. Alcohol precipitation purification: mixtures A, B and C are combined, 15 parts of anhydrous ethanol is added, stirring is performed at 50℃ for 20 min to promote dissolution, low-temperature precipitation is performed by placing in a refrigerator at 5℃ for 10 h, and finally, the extract of Atractylodes macrocephala Koidz is obtained by filtration. After drying, the extract is packaged for use.

[0048] 3. The extraction method of the extract of Poria cocos, comprising the following steps:

[0049] 3.1 Hot water extraction: 1 part of Poria cocos powder is weighed and added to 20 parts of hot water for extraction;

[0050] 3.2 Preliminary treatment: the solid-liquid two phases are separated by filtration, and the filtrate is retained; the filtrate is concentrated (distilled) under reduced pressure, and the ratio of the extraction solution to the concentrated solution is controlled to be 10:1;

[0051] 3.3 Enzymatic hydrolysis: cellulase 0.0001 parts, neutral protease 0.0001 parts and pectinase 0.0001 parts are added to the concentrated solution, and enzymatic hydrolysis is performed at 35℃ for 24 hours;

[0052] 3.4 alcohol precipitation: add 95% ethanol to the concentrated solution, and place it at 5°C for 12 hours;

[0053] 3.5 separation and washing: centrifugal separation of the precipitate, and then sequentially wash the precipitate with anhydrous ethanol, acetone and ether;

[0054] 3.6 final treatment: vacuum drying of the washed precipitate to obtain the extract of Poria cocos polysaccharide.

[0055] 4. The extraction method of licorice extract, comprising the following steps:

[0056] 4.1 raw material pretreatment: take 1 part of licorice, cut into small pieces and add 10 parts of water, soak for 2 hours;

[0057] 4.2 decoction and concentration: heat decoction for 0.5-3 hours, until the volume of the concentrated solution reaches 1 / 3 of the water added;

[0058] 4.3 solid-liquid separation: precipitate and take the supernatant;

[0059] 4.4 refining and drying: filter the supernatant, and spray dry the filtrate to obtain licorice extract.

[0060] 5. The extraction method of psoralen extract, comprising the following steps:

[0061] 5.1 raw material pretreatment: cut or grind the psoralen sample into fine powder;

[0062] 5.2 alcohol extraction: add 95% ethanol according to the solid-liquid ratio of 1:5-1:10, soak at room temperature for 24-48 hours;

[0063] 5.3 ultrasonic extraction: place the extraction solution in an ultrasonic extractor, set the power to 300W, extract for 30 minutes, and control the temperature at 40°C;

[0064] 5.4 filtration and concentration: filter with a 0.45μm filter membrane, and concentrate the filtrate under reduced pressure at 50°C to 1 / 5 of the original volume

[0065] 5.5 drying treatment: grind the concentrated solution to pass through an 80 mesh sieve after vacuum drying (60°C, 0.08MPa);

[0066] 5.6 quality control: detect the content of psoralen ≥2.0%, and the moisture content ≤5%.

[0067] 6. The extraction method of cistanche extract, comprising the following steps:

[0068] 6.1 raw material pretreatment: dry the cistanche medicinal material at 60°C, and grind it into a 40 mesh powder;

[0069] 6.2D-mannitol extraction: reflux extraction with 10 times amount of 95% ethanol for 3 times (2 hours each time), and the extract was concentrated and crystallized to obtain D-mannitol;

[0070] 6.3 phenylethanoid glycoside extraction: the residue was extracted with 8 times amount of 70% ethanol, and the extract was subjected to AB-8 macroporous resin column (gradient elution with ethanol), and the elution fraction of 30% ethanol was collected, concentrated and dried;

[0071] 6.4 polysaccharide extraction: the residue was extracted with 12 times amount of water for 3 times (1 hour each time), and the combined extract was concentrated, 3 times volume of 95% ethanol was added, precipitated at 4°C for 24 hours, centrifuged to obtain the precipitate which was dried;

[0072] 6.5 preparation of finished product: mixed according to the ratio of D-mannitol: phenylethanoid glycoside: polysaccharide = 1:2:3 to obtain Cistanche composite extract.

[0073] 7, the extraction method of Eucommia ulmoides Oliv. extract, comprising the following steps:

[0074] 7.1 raw material treatment: Eucommia ulmoides Oliv. bark was crushed through 40 mesh sieve;

[0075] 7.2 alcohol extraction: extracted with 60% ethanol (solid-liquid ratio 1:15) at 60°C for 2 times (30 minutes each time);

[0076] 7.3 purification: the combined extract was subjected to neutral alumina column (100-200 mesh) for decolorization, and eluted with 95% ethanol;

[0077] 7.4 concentration and drying: the eluate was extracted with ethyl acetate for 3 times, the combined organic phase was concentrated under reduced pressure, and spray dried to obtain Eucommia ulmoides Oliv. extract.

[0078] 8, the extraction method of Angelica sinensis polysaccharide: completely consistent with the extraction method of Codonopsis pilosula polysaccharide (1.1-1.8 steps).

[0079] S2, the dry matter of each traditional Chinese medicine extract prepared in S1 was mixed according to the following mass formula to prepare Jiawei Sijunzi Decoction: Codonopsis pilosula polysaccharide extract 15-20, Atractylodes macrocephala Koidz extract 15-20, Grifola frondosa polysaccharide 5-10, Glycyrrhiza uralensis Fisch extract 5-10, Fructus Psoraleae extract 15-20, Cistanche tubulosa extract 15-20, Eucommia ulmoides Oliv. extract 15-20, Angelica sinensis polysaccharide extract 15-20;

[0080] S3, the dry matter of each traditional Chinese medicine extract prepared in S1 was mixed according to the following mass formula to prepare classic Sijunzi Decoction: Codonopsis pilosula polysaccharide 15-20, Atractylodes macrocephala Koidz extract 15-20, Grifola frondosa polysaccharide 5-10, Glycyrrhiza uralensis Fisch extract 5-10. After the raw materials of each formula were mixed uniformly, they were packaged separately for use.

[0081] Example 2

[0082] This embodiment provides a method for preparing a liquid multi-dimensional composite nutrient for livestock and poultry, which comprises the following steps:

[0083] S1. Preparation of oil phase raw materials:

[0084] (1) The oil phase raw materials are composed of the following components in a mass ratio: 2 parts of vitamin A, 30.1 parts of vitamin D, 8 parts of vitamin E, 0.6 parts of tea polyphenols, 10 parts of glycerol, 3 parts of fish oil, 1 part of potassium hydroxide, and 75.3 parts of Tween-80, for a total of 100 parts;

[0085] (2) Heat the fish oil to melt and mix well;

[0086] (3) adding a 50% potassium hydroxide aqueous solution to the fish oil at a rate of 18 to 28 drops / min;

[0087] (4) After the addition was complete, the mixture was stirred at 50°C and 70 rpm for 35 min;

[0088] (5) Add the remaining oil phase raw materials to the above emulsion, keep warm and stir at 60-68°C until a clear and uniform light yellow transparent liquid is formed to obtain an oil phase material mixture containing an emulsifier.

[0089] S2. Preparation of aqueous phase material:

[0090] (1) The aqueous phase raw materials are composed of the following components in a mass ratio: 2 parts of vitamin B1, 0.5 parts of vitamin B2, 0.5 parts of vitamin B6, 1 part of biotin-vitamin B7, 1 part of folic acid-vitamin B 11 1 serving, Vitamin B 12 1 part, 10 parts of vitamin C, 1.5 parts of lysine, 3 parts of methionine, 12 parts of taurine, 2 parts of modified Sijunzi Decoction, 0.1 parts of potassium hydroxide, and 65.4 parts of sterile deionized water, a total of 100 parts; specifically, the mass ratio of the modified Sijunzi Decoction is: 18 parts of Codonopsis pilosula polysaccharide extract, 18 parts of Atractylodes macrocephala extract, 8 parts of Poria cocos polysaccharide, 8 parts of Licorice extract, 18 parts of Psoralea corylifolia extract, 18 parts of Cistanche deserticola extract, 18 parts of Eucommia ulmoides extract, and 18 parts of Angelica sinensis polysaccharide extract;

[0091] (2) Mixing and stirring the aqueous phase raw materials until the mixture is clear and transparent to obtain an aqueous phase material mixture.

[0092] S3, emulsification reaction:

[0093] (1) Slowly add the aqueous phase obtained in S2 to the oil phase obtained in S1 in 5 portions, with each addition ratio being 20%;

[0094] (2) The mass ratio of oil phase raw material to water phase raw material is 1:4;

[0095] (3) Keep stirring at 60-68°C for 60 min;

[0096] (4) detecting and adjusting the pH to 5 to obtain a multivitamin nutrient solution colostrum;

[0097] (5) subjecting the primary emulsion obtained in (4) to a high-pressure homogenization treatment, wherein the high-pressure homogenization treatment is performed by a high-pressure homogenizer under 110 MPa conditions for 4 cycles and maintaining a constant temperature of 60° C. to obtain a nanoemulsion with a particle size of 20 to 30 nm;

[0098] (6) filtering the nanoemulsion obtained in (5) through a 0.11-0.22 μm filter element;

[0099] (7) Aseptically package the product into two sizes: 500ml and 1000ml.

[0100] Example 3

[0101] This embodiment provides a method for preparing a liquid multi-dimensional composite nutrient for livestock and poultry, which differs from Example 2 in that:

[0102] S1. Preparation of oil phase raw materials:

[0103] (1) The oil phase raw materials are composed of the following components in a mass ratio: 3 parts of vitamin A, 0.2 parts of vitamin D3, 10 parts of vitamin E, 0.8 parts of tea polyphenols, 15 parts of glycerol, 4 parts of fish oil, 2 parts of potassium hydroxide, and 65 parts of Tween-80, for a total of 100 parts;

[0104] S2. Preparation of aqueous phase material:

[0105] (1) The aqueous phase raw materials are composed of the following components in a mass ratio: 2.5 parts of vitamin B1, 0.6 parts of vitamin B2, 0.6 parts of vitamin B6, 1.5 parts of biotin-vitamin B7, 1.5 parts of folic acid-vitamin B 11 1.5 portions, vitamin B 12 1.5 parts, 12 parts of vitamin C, 2 parts of lysine, 3.5 parts of methionine, 15 parts of taurine, 2.5 parts of modified Sijunzi Decoction, 0.2 parts of potassium hydroxide, and 56.6 parts of sterile deionized water, a total of 100 parts; the specific mass ratio of the modified Sijunzi Decoction is the same as that in Example 2;

[0106] S3, emulsification reaction:

[0107] (2) The mass ratio of oil phase raw material to water phase raw material is 1:5;

[0108] (4) detecting and adjusting the pH to 6.3 to obtain a multivitamin nutrient solution colostrum;

[0109] (5) the initial emulsion obtained in (4) is subjected to high-pressure homogenization treatment, the high-pressure homogenization treatment is cyclic treatment for 3 times under the condition of 100 MPa by using a high-pressure homogenizer, and constant temperature of 60°C is maintained, to obtain a nano-emulsion with particle size of 20-30 nm.

[0110] Other step parameters not specifically mentioned are consistent with Example 2.

[0111] Example 4

[0112] The present example provides a preparation method of liquid multi-vitamin complex nutrient special for livestock and poultry, which is different from Example 2 in that:

[0113] S1, oil phase raw material preparation:

[0114] (1) the oil phase raw materials are composed of vitamin A 4 parts, vitamin D3 0.3 parts, vitamin E 15 parts, tea polyphenol 1 part, glycerol 20 parts, fish oil 6 parts, potassium hydroxide 3 parts, and Tween-80 50.7 parts, with a total of 100 parts;

[0115] S2, water phase material preparation:

[0116] (1) the water phase raw materials are composed of vitamin B1 3 parts, vitamin B2 0.8 parts, vitamin B6 0.8 parts, vitamin B7-biotin 2 parts, vitamin B12 0.3 parts, folic acid 2 parts, vitamin B6 2 parts, vitamin C 15 parts, lysine 3 parts, methionine 4 parts, taurine 18 parts, flavored Sijunzi decoction 3 parts, potassium hydroxide 0.3 parts, and sterile deionized water 46.1 parts, with a total of 100 parts; the specific mass ratio of the flavored Sijunzi decoction is the same as that of Example 2; 11 12 2 parts, vitamin C 15 parts, lysine 3 parts, methionine 4 parts, taurine 18 parts, flavored Sijunzi decoction 3 parts, potassium hydroxide 0.3 parts, and sterile deionized water 46.1 parts, with a total of 100 parts; the specific mass ratio of the flavored Sijunzi decoction is the same as that of Example 2;

[0117] S3, emulsification reaction:

[0118] (2) the mass ratio of oil phase raw materials to water phase raw materials is 1:6;

[0119] (4) detect and adjust the pH to 3.4 to obtain a complex vitamin nutrient liquid initial emulsion;

[0120] (5) the initial emulsion obtained in (4) is subjected to high-pressure homogenization treatment, the high-pressure homogenization treatment is cyclic treatment for 3 times under the condition of 100 MPa by using a high-pressure homogenizer, and constant temperature of 60°C is maintained, to obtain a nano-emulsion with particle size of 20-30 nm.

[0121] Other step parameters not specifically mentioned are consistent with Example 2.

[0122] Comparative Example 1

[0123] ​The comparative example provides a preparation method of a liquid multi-vitamin compound nutrient special for livestock and poultry, which is different from example 2 in that the water phase material preparation in step S2 is replaced by a classic four gentlemen decoction instead of flavored four gentlemen decoction. Specifically, the water phase raw materials are composed of the following components in a mass ratio: vitamin B1 2 parts, vitamin B20.5 parts, vitamin B60.5 parts, biotin-vitamin B7 1 part, folic acid-vitamin B 11 1 part, vitamin B 12 1 part, vitamin C 10 parts, lysine 1.5 parts, methionine 3 parts, taurine 12 parts, classic four gentlemen decoction 2 parts, potassium hydroxide 0.1 part, sterile deionized water 65.4 parts, a total of 100 parts; wherein the classic four decoction is configured according to the following component mass ratio: 18 of radix angelicae sinensis polysaccharide extract, 18 of atractylodes extract, 8 of grifola frondosa polysaccharide, and 8 of glycyrrhiza extract.

[0124] Comparative example 2

[0125] The comparative example provides a preparation method of a liquid multi-vitamin compound nutrient special for livestock and poultry, which is different from example 2 in that no traditional Chinese medicine preparation flavored four gentlemen decoction is added in the water phase material in step S3, and the proportion of the remaining components remains unchanged, and the sterile deionized water is correspondingly increased to 67.4 parts to maintain the total amount consistent.

[0126] Comparative example 3

[0127] The comparative example provides a preparation method of a liquid multi-vitamin compound nutrient special for livestock and poultry, which is different from example 2 in that the traditional water decoction method is used to extract the traditional Chinese medicine preparation flavored four gentlemen decoction; because the traditional extract contains more macromolecular impurities, the emulsion in step S3 is first filtered by a 1 μm filter, and then filtered again by a 0.45 μm filter.

[0128] Comparative example 4

[0129] The comparative example provides a preparation method of a liquid multi-vitamin compound nutrient special for livestock and poultry, which is different from example 2 in that the high-pressure homogenization treatment is cancelled in the emulsification reaction in S3, and only ordinary stirring emulsification is used; because the emulsion particle size is greater than 500 nm in step S6, it cannot pass through a 0.22 μm sterile filter, so it is coarsely filtered through a 5 μm pre-filtering filter.

[0130] The contents of the effective components in the liquid multi-vitamin compound nutrient special for livestock and poultry prepared by the methods of examples 2-4 and comparative examples 1-4 are detected by the following method, and the results are shown in Table 1.

[0131] The specific detection criteria of each component are as follows: Vitamin A acetate is determined according to the saponification extraction method in GB / T 17817-2010; Vitamin D3 refers to the saponification extraction method in GB / T 17818-2010; Vitamin E is determined according to the saponification extraction method (arbitration method) in GB / T 17812-2008; Vitamin B1 and B2 are determined by methods 2 in GB / T 14700-2018 and GB / T 14701-2019 respectively; Vitamin B6 and B 12 respectively according to GB / T 14702-2018 and GB / T 17819-2017; Folic acid-vitamin B 11 and biotin-vitamin B7 content are determined according to GB / T 17813-2018 and GB / T 17778-2005 standards respectively; Lysine and methionine are determined according to GB / T 18246-2019.

[0132] Table 1: Content of each effective component in the livestock and poultry special-purpose multi-vitamin and multi-mineral prepared in Examples 2-4 and Comparative Examples 1-4

[0133]

[0134]

[0135] Test Example 1

[0136] The average particle size and stability of the livestock and poultry special-purpose liquid multi-vitamin and multi-mineral prepared in Examples 2-4 and Comparative Examples 1-4 were tested, and the results are shown in Table 2.

[0137] 1. The average particle size of the nanoemulsion was determined by dynamic light scattering method (DLS). The sample was irradiated with laser at a scattering angle of 90° at 25±1℃, and the particle size distribution was determined by detecting the change of light scattering intensity. Each sample was tested repeatedly for 3 times and the average value was taken.

[0138] 2. The stability of the nanoemulsion was evaluated by 45℃ constant temperature accelerated test. After the sample was stored for 60 days, the change of the content of each vitamin was determined by high performance liquid chromatography method. The loss rate of each vitamin was calculated according to the formula (initial content-60 days later content) / initial content×100%, and the total loss rate was obtained by adding the loss rates of all vitamins. The smaller the value, the better the stability of the system.

[0139] Table 2: Comparison of average particle size and total vitamin loss rate of nanoemulsions in different groups

[0140]

[0141]

[0142] From the data in Table 2, the particle size analysis: the average particle size of examples 2-4 is maintained below 30 nm (20-30 nm), this nano-scale particle size distribution can significantly improve the bioavailability of nutrients, make the fat-soluble vitamins uniformly dispersed in the water phase, form a stable O / W type nano-emulsion system; after 60 days of 45℃ accelerated test, the total loss rate of all examples is less than 0.20%, and the compatibility system of traditional Chinese medicine extract and vitamin does not appear precipitation or stratification phenomenon, fully proves that the liquid multi-vitamin compound nutrient system proposed in the application has the vitamin activity maintaining ability and oil-water phase stability.

[0143] Animal experiment 1

[0144] The liquid multi-vitamin compound nutrients prepared by examples 2 and comparative examples 1-4 are actually carried out in nursery pig breeding test.

[0145] (1) Test time and place: the test was carried out in the nursery house of Shandong Xibeiaizhu Pig Farm from June 20, 2024 to July 26, a total of 36 days;

[0146] (2) Test animals: 360 healthy weaned nursery pigs of 24 days old were selected as test objects, all test pigs were from the same source, with the same parity, similar farrowing time, litter size, and sex ratio, and the weaning weight was 6.5±1.10 kg;

[0147] (3) Test grouping is divided into 6 groups: test group 1 (using the product of example 2 group), test group 2 (using the product of comparative example 1 group), test group 3 (using the product of comparative example 2), test group 4 (using the product of comparative example 3 group), test group 5 (using the product of comparative example 4 group), blank control group (synchronous with mass production, without liquid multi-vitamin), each group is set with 3 repeats, and each repeat is 20 pigs.

[0148] (4) Add corresponding liquid multi-vitamin compound nutrients for livestock and poultry in drinking water, add 1 kg of liquid multi-vitamin compound nutrients for livestock and poultry per ton of water, add 1 time per day, and all pigs are free to eat.

[0149] (5) Record the diarrhea death and growth rate of nursery pigs in control group and test group within 36 days of test period, and the results are shown in Table 3 and Table 4.

[0150] Table 3, comparison of diarrhea death of nursery pigs in each test group

[0151]

[0152]

[0153] Table 4 shows the influence of different test groups on the growth rate of nursery pigs

[0154] Test item Control group Test 1 group Test 2 group Test 3 group Test 4 group Test 5 group Age at test start 24 24 24 24 24 24 Age at test end 60 60 60 60 60 60 Average weight at test start (kg / head) 7.8 6.8 5.8 7.9 6.9 6.5 Average weight at test end (kg / head) 17.5 18.8 16.8 18.2 17 17 Average daily gain (g / head·day) 270 333.3 305.5 286.1 280.5 291.6 Average daily feed intake (g / head·day) 302 320 310 308 310 308 Feed conversion ratio 1.1:1 0.96:1 1:01 1.1:1 1.1:1 1.1:1

[0155] From Table 3 and Table 4, it can be seen that the test group 1 (product of Example 2) showed the best comprehensive performance in the 36-day test period: the average daily weight gain of the nursery pigs reached 333.3 g / pig / day, which was 23.4% higher than the control group; the feed conversion ratio was 0.96:1, which was better than the control group of 1.1:1; at the same time, the mortality rate (1.7%) and the incidence of diarrhea (3%) were the lowest in each group, which were 87% and 85% lower than the control group, respectively. This shows that the liquid multi-vitamin compound nutrient provided by the present application can significantly promote the growth of nursery pigs, improve the feed conversion efficiency, and effectively reduce the incidence of disease, and has good breeding application value.

[0156] (6) Immune organ development evaluation: At 24 days and 60 days of age, 5 healthy nursery pigs were randomly selected from each group, and were fasted for 12 hours (with free water) before being weighed. Then the experimental animals were sacrificed by carotid artery bleeding method, and the thymus (located from the neck to the precordial region), spleen (located behind the greater curvature of the stomach on the left abdominal side) and mesenteric lymph nodes of the jejunum were immediately dissected. The collected organs need to be carefully removed from the surface connective tissue and fat, and finally weighed accurately using an electronic balance. The results are shown in Table 5, and the immune organ index (mg / g) = net weight of immune organ (mg) / live body weight (g).

[0157] Table 5: Effect of different experimental groups on immune organ index of nursery pigs

[0158]

[0159]

[0160] As can be seen from Table 5, the thymus, spleen and mesenteric lymph node indices of 60-day-old nursery pigs in the test group 1 (using the product of Example 2) reached 2.3, 2.6 and 1.4 mg / g, respectively, which were significantly increased by 155.6%, 73.3% and 75% compared with the control group, and it was the only group that achieved synchronous development of all immune organs, indicating that the liquid multi-vitamin compound nutrient of the present application can effectively promote the development of immune organs of nursery pigs and improve the immune function of the body.

[0161] Animal experiment 2

[0162] Egg-laying chicken breeding experiments were carried out on the liquid multi-vitamin compound nutrients prepared in Example 2 and Comparative Examples 1-4.

[0163] (1) Evaluation of special liquid multi-vitamin compound nutrients for livestock and poultry on production performance in the post-egg-laying period

[0164] The test was carried out in Yancheng Chenbao Poultry Farm, and 380-day-old healthy laying hens were selected as the test objects. The test started at 380 days of age, and ended at 408 days of age, for a total of 28 days. All test laying hens were of the same origin, breed, and had similar egg-laying periods and health conditions. The test used a completely randomized block design, with a total of 900 chickens divided into 6 experimental groups: test group 1 (using the product of example 2), test group 2 (using the product of comparative example 1), test group 3 (using the product of comparative example 2), test group 4 (using the product of comparative example 3), test group 5 (using the product prepared in comparative example 4), and a blank control group (without liquid multivitamins, managed according to the large-scale production mode), with 3 replicates of 50 chickens each.

[0165] Each test group was caged, and the granular feed was the same in each group. The chickens were allowed to freely eat and drink water, and the coop was cleaned regularly. The laying hens in each test group were given the multivitamin product prepared in the corresponding example or comparative example in their drinking water, with 1 ml of the liquid multivitamin compound for livestock and poultry added to each 1 L of drinking water. The addition was made once a day, and the laying hens were allowed to freely eat. The blank control group was not given any addition, and the experimental results are shown in Table 6 below.

[0166] Table 6, Effect of liquid multivitamin compound for livestock and poultry on production performance in the late laying period

[0167]

[0168]

[0169] Generally, after laying hens reach 380 days of age, their physical fitness gradually declines, and most of them are in a sub-healthy state. Therefore, their egg-laying rate gradually decreases, and the rate of unqualified eggs gradually increases. However, as can be seen from the results in Table 6, after test group 1 used the new type of compound vitamin nutrient liquid of the present application, the egg-laying rate was stably maintained at 94.8%, and the breakage rate was only 0.5%, which was better than the other test groups, showing good economic benefits.

[0170] (2) Evaluation of immune organ development in laying hens

[0171] Select 120 healthy 14-day-old laying chicks in the Chenbao laying farm, and divide them into 6 groups, 20 in each group: test group 1 (using the product of example 2), test group 2 (using the product of comparative example 1), test group 3 (using the product of comparative example 2), test group 4 (using the product of comparative example 3), test group 5 (using the product prepared in comparative example 4), and a blank control group (without using liquid multivitamins, and managed according to the large production mode). Each test group is caged, and the granular feed is the same in each group. The chickens are free to eat and drink water, and the henhouse is regularly cleaned. The laying hens in each test group are added with the multivitamin product prepared in the corresponding example or comparative example in the drinking water. 1 ml of liquid multivitamin compound for livestock and poultry is added to each 1 L of drinking water, added once a day, and the laying chicks are free to eat.

[0172] At 14 days and 28 days, 5 healthy laying chicks are randomly selected from each group, and the fasting body weight is measured after fasting for 12 hours (free drinking water during the period). After the laying chicks are killed by the heart air embolism method, the spleen, bursa of fabricius and thymus tissues are immediately dissected and weighed after removing the surface connective tissue and fat. Finally, the development index of each immune organ is calculated, and the results are shown in Table 7. Immune organ index (mg / g) = net weight of immune organ (mg) / live fasting body weight (g).

[0173] Table 7: Effect of different experimental groups on immune organ index of laying chicks

[0174]

[0175]

[0176] As can be seen from the data in Table 7, test group 1 (product of example 2) significantly promotes the development of immune organs of laying hens: the thymus, spleen and bursa of fabricius indexes at 14 days of age are 1.2, 0.5 and 0.7 mg / g, respectively, which are 20%, 67% and 17% higher than those of the control group; and at 28 days of age, they are further increased to 1.7, 0.7 and 0.8 mg / g, which are 89%, 40% and 33% higher than those of the control group. The immune organ index of test group 1 remains the highest at all detection time points, indicating that the liquid multivitamin compound provided by the example of the present application can continuously promote the development of the immune system of laying hens in the early stage, and the effect is significantly better than that of other test groups.

[0177] Obviously, the above-described embodiments are only some embodiments but not all the embodiments of the present application, the present application can be implemented in many different forms, and the purpose of providing these embodiments is to make the disclosure of the present application more thorough and comprehensive. Although the present application is described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions recorded in the foregoing specific embodiments, or make equivalent replacements to some technical features. Any equivalent structure made by using the content of the present application, directly or indirectly used in other related technical fields, is also within the scope of the patent protection of the present application.

Claims

1. A method for preparing a liquid multidimensional composite nutrient for livestock and poultry, characterized in that: The following steps are involved: (1) preparing an oil phase comprising fat-soluble vitamins, an emulsifier and fish oil; (2) preparing an aqueous phase comprising water-soluble vitamins, amino acids, and a traditional Chinese medicine preparation, wherein the traditional Chinese medicine preparation is a traditional Chinese medicine preparation of modified Sijunzi Decoction; (3) Add the water phase to the oil phase in batches for emulsification reaction, with the mass ratio of oil phase to water phase being 1:4-6; (4) performing high-pressure homogenization on the emulsion to obtain a nano-scale emulsion, wherein the particle size of the emulsion is less than 30 nm; (5) Filter and sterilize before packaging.

2. The method for preparing the liquid multidimensional composite nutrient for livestock and poultry according to claim 1, characterized in that: The oil phase comprises the following components in parts by mass: 2-4 parts of vitamin A, 0.1-0.3 parts of vitamin D3, 8-15 parts of vitamin E, 0.6-1 parts of tea polyphenols, 10-20 parts of glycerol, 3-6 parts of fish oil, 1-3 parts of potassium hydroxide, and 50-75.3 parts of Tween-80.

3. The method for preparing the liquid multidimensional composite nutrient for livestock and poultry according to claim 1, characterized in that: The aqueous phase contains the following components in parts by mass: 2-3 parts of vitamin B1, 0.5-0.8 parts of vitamin B2, 0.5-0.8 parts of vitamin B6, 1-2 parts of biotin-vitamin B7, 1-2 parts of folic acid-vitamin B 11 1-2. Vitamin B 12 1-2, vitamin C 10-15, lysine 1.5-3, methionine 3-4, taurine 12-18, traditional Chinese medicine preparation 2-3, potassium hydroxide 0.1-0.3, sterile deionized water 40-70.

4. The method for preparing the liquid multidimensional composite nutrient for livestock and poultry according to claim 1, characterized in that: In the emulsification reaction step, the water phase is added to the oil phase in 5 times.

5. The method for preparing the liquid multidimensional composite nutrient for livestock and poultry according to claim 3, characterized in that: The traditional Chinese medicine preparation modified Sijunzi decoction consists of codonopsis pilosula polysaccharide extract, atractylodes macrocephala extract, poria cocos polysaccharide extract, liquorice extract, psoralea corylifolia extract, cistanche deserticola extract, eucommia bark extract and angelica polysaccharide extract.

6. The method for preparing the liquid multi-dimensional composite nutrient for livestock and poultry according to claim 5, characterized in that: The traditional Chinese medicine preparation modified Sijunzi Decoction contains the following components by weight: 15-20 grams of Codonopsis pilosula polysaccharide extract, 15-20 grams of Atractylodes macrocephala extract, 5-10 grams of Poria cocos polysaccharide, 5-10 grams of Licorice extract, 15-20 grams of Psoralea corylifolia extract, 15-20 grams of Cistanche deserticola extract, 15-20 grams of Eucommia ulmoides extract, and 15-20 grams of Angelica sinensis polysaccharide extract.

7. The method according to claim 1, characterized in that The high-pressure homogenization treatment is specifically carried out under the conditions of 100-120 MPa and 60-68° C. for 3-5 cycles.

8. The method according to claim 7, characterized in that The emulsification reaction is carried out at 60-68° C., and the pH is adjusted to 3.4-6.3; and the filtration adopts a 0.11-0.22 μm filter membrane.

9. A liquid multi-dimensional composite nutrient for livestock and poultry prepared by the method according to any one of claims 1 to 8, characterized in that: It contains nanoemulsion with particle size of 20 to 30 nm.

10. An application of the liquid multi-dimensional composite nutrient for livestock and poultry according to claim 9, characterized in that: The following applications are included: (1) Application in pig farming: Add to the pig drinking water system at a ratio of 1 kg / ton of water, once a day; (2) Application in poultry farming: Add to the drinking water system of laying hens at a ratio of 1 ml / L water, once a day; (3) The application method is suitable for nutritional supplementation throughout the entire growth cycle of livestock and poultry.

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