Anti-aging composition for reducing pressure type fatigue and preparation method and application thereof

Abstract

The invention relates to the technical field of cosmetics, and particularly discloses an anti-aging composition for reducing pressure type fatigue as well as a preparation method and application of the anti-aging composition. The anti-aging composition comprises a camellia nitidissima extract, a tephrosia sinensis seed extract, mu-conopeptide and ascorbic acid polypeptide, the mass ratio of the camellia nitidissima extract to the tephrosia sinensis seed extract to the mu-conopeptide to the ascorbic acid polypeptide is (0.1-5): (0.01-2): (0.001-1): (0.01-5). The anti-aging composition provided by the invention improves the problems of dull skin, rough skin, loose skin and fine wrinkles caused by staying up late, working and mood pressure by reducing skin cell pressure injury and improving cell viability, and is mild, non-irritant and suitable for people with sensitive skin to use.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of cosmetics, in particular to an anti-aging composition for reducing stress fatigue and a preparation method and application thereof. BACKGROUND

[0002] Under the heavy pressure of fast-paced life, the skin is subjected to internal and external attacks far beyond the naked eye, forming a typical "stress muscle" state. When the cells are deprived of the golden period of night repair due to staying up late, the accumulation of metabolic waste and slow microcirculation make the skin lose its natural luster and appear stubborn dullness. When the cortisol storm is continuously triggered by work fatigue, collagen and elastic fibers are accelerated to be consumed, and the subcutaneous support structure collapses like a boat without anchor, leading to sagging and falling contour lines. When the barrier defense line is repeatedly impacted by emotional stress, the keratin layer's water retention capacity collapses, making the originally fine expression lines evolve into persistent fragile fine lines, and finally interweave into a complex fatigue of roughness, collapse and dryness. The essence of this "stress muscle" is the result of long-term erosion of cell vitality by sleep deprivation, metabolic disorder, collagen loss and barrier fragility.

[0003] However, the current mainstream anti-aging technology still mainly targets the natural aging mechanism, and it is difficult to meet the core needs of multi-pathway collaborative repair and mildness for stress-type skin aging. For example, in the selection of target sites, excessive focus is placed on a single pathway that promotes collagen synthesis, while the reduction of cell vitality, metabolic accumulation and chronic inflammation in stress-type aging are ignored. In the application of active ingredients, traditional anti-wrinkle ingredients such as retinoids can improve photoaging wrinkles, but their strong keratolytic effect will further damage the barrier integrity of the already damaged stress muscle. SUMMARY

[0004] The present application aims to overcome the shortcomings of the prior art and provide an anti-aging composition for reducing stress fatigue and a preparation method and application thereof. The anti-aging composition provided by the present application reduces skin cell stress damage and improves cell vitality, thereby improving skin dullness, roughness, sagging and fine line problems caused by staying up late, work and emotional stress, and is mild and non-irritating, suitable for sensitive skin.

[0005] To achieve the above-mentioned purpose, the technical solution adopted by the present application is as follows:

[0006] The present application provides an anti-aging composition, which comprises a Lycium ruthenicum Murr extract, a Prosopis cineraria seed extract, a μ-conotoxin and an ascorbic acid polypeptide.

[0007] The mass ratio of the Lycium ruthenicum Murr extract, the Prosopis cineraria seed extract, the μ-conotoxin and the ascorbic acid polypeptide is (0.1-5):(0.01-2):(0.001-1):(0.01-5).

[0008] Through the above technical solution, the four active components of Camellia japonica extract, Prosopis cineraria seed extract, mu-conotoxin and ascorbic acid polypeptide form a cascade anti-aging network after penetration, and comprehensively improve the dull, rough, loose and fine line problems caused by fatigue and stress. Among them, Camellia japonica extract is rich in tea polyphenols and flavonoid glycosides, which can stimulate collagen synthesis and antioxidant, protect skin cells from stress damage, and significantly improve skin sagging; Prosopis cineraria seed extract is rich in polysaccharides and flavone glycosides, which can reduce the level of stress hormone cortisol and relieve skin stress; mu-conotoxin is a kind of neuroregulatory peptide, which can inhibit muscle contraction signal pathway and reduce dynamic muscle tension, and significantly improve expression lines and fine lines; ascorbic acid polypeptide is a complex of vitamin C and polypeptide, which can effectively antioxidant, whitening and promote collagen regeneration, and significantly improve skin dullness, roughness and signs of aging. The inventors of the present application found through a large number of research and experiments that Camellia japonica extract and Prosopis cineraria seed extract have a synergistic penetration effect, and the polyphenolic compounds in Camellia japonica extract can enhance the lipid fluidity by interfering with the lipid arrangement of skin cells, promote the penetration of lipophilic ingredients; the polysaccharides in Prosopis cineraria seed extract can improve the hydration of the skin, form a hydrogel layer to improve the hydration of the stratum corneum, and reduce the diffusion resistance of hydrophilic compounds; the flavonoids in Camellia japonica extract and Prosopis cineraria seed extract can expand the intercellular tight junction by affecting the expression of tight junction proteins, and assist the penetration of macromolecular peptides.

[0009] Moreover, the present application realizes breakthrough intervention of stress-induced skin aging through the following comprehensive advantages: at the mechanism level, a multi-target synergistic pathway is creatively constructed — the flavone glycosides in Prosopis cineraria seed extract directly down-regulate the level of cortisol, thereby blocking the collagen degradation and barrier damage induced by stress hormones from the source; at the same time, Camellia japonica extract and ascorbic acid polypeptide form a dual antioxidant matrix, which can synergistically neutralize the reactive oxygen species (ROS) triggered by overwork and emotional stress, and promote collagen regeneration; and mu-conotoxin precisely inhibits the calcium ion channel of motor neurons, thereby relieving the dynamic wrinkle fixation caused by the continuous contraction of expression muscles. The four components of Camellia japonica extract, Prosopis cineraria seed extract, mu-conotoxin and ascorbic acid polypeptide form a cascade anti-aging network of "stress blocking-oxidation scavenging-collagen remodeling-muscle relaxation".

[0010] At the level of transdermal absorption of active substances, the polyphenolic components in Camellia japonica extract enhance the lipid fluidity of cell membranes, the hydrated gel layer formed by Prosopis cineraria polysaccharides improves the permeability of the stratum corneum, and the flavonoid components of the two synergistically regulate the expression of tight junction proteins to expand the intercellular space, three mechanisms significantly optimize the transdermal efficiency of active ingredients such as mu-conotoxin and ascorbic acid polypeptide; at the safety level, the compatibility of active components through natural plant extracts (Camellia japonica / Prosopis cineraria) and directed modification peptides avoids traditional strong stimulating ingredients (such as retinol, high-concentration acid), and sensitive skin groups can also use it.

[0011] The Camellia sinensis extract, the Millettia pinnata seed extract, the mu-conotoxin and the ascorbic acid polypeptide in the above mass ratio can reduce skin cell stress damage, improve cell vitality, thereby improving skin dullness, roughness, sagging and fine line problems caused by staying up late, work and emotional stress, and are mild and non-irritating, suitable for people with sensitive skin.

[0012] As a preferred embodiment of the anti-aging composition described in the present application, the mass ratio of the Camellia sinensis extract, the Millettia pinnata seed extract, the mu-conotoxin and the ascorbic acid polypeptide is (0.5-3):(0.05-0.5):(0.01-0.5):(0.1-2).

[0013] In the technical solution of the present application, the Camellia sinensis extract, the Millettia pinnata seed extract, the mu-conotoxin and the ascorbic acid polypeptide are in the above preferred mass ratio range, the Camellia sinensis extract and the Millettia pinnata seed extract can better synergize with the mu-conotoxin and the ascorbic acid polypeptide to promote penetration, and then better form a "stress blocking-oxidation scavenging-collagen remodeling-muscle relaxation" cascade anti-aging network with the mu-conotoxin and the ascorbic acid polypeptide, and comprehensively improve the dullness, roughness, sagging and fine line problems caused by fatigue stress.

[0014] As a preferred embodiment of the anti-aging composition described in the present application, the mass ratio of the Camellia sinensis extract, the Millettia pinnata seed extract, the mu-conotoxin and the ascorbic acid polypeptide is (1-2):(0.2-0.3):(0.1-0.2):(0.6-0.8).

[0015] When the mass ratio of the Camellia sinensis extract, the Millettia pinnata seed extract, the mu-conotoxin and the ascorbic acid polypeptide in the present application is more preferably in the above range, a "stress blocking-oxidation scavenging-collagen remodeling-muscle relaxation" cascade anti-aging network is better formed, and the dullness, roughness, sagging and fine line problems caused by fatigue stress are comprehensively improved.

[0016] In the present application, the Camellia sinensis extract and the ascorbic acid polypeptide form a two-dimensional antioxidant matrix, synergistically neutralize active oxygen (ROS) stimulated by staying up late and emotional stress, and promote collagen neogenesis, and the above components synergistically matched can better stimulate the synthesis of collagen, improve the antioxidant effect; when applied to an anti-aging composition, it can better protect skin cells from stress damage, significantly improve skin sagging, and achieve anti-aging effect.

[0017] As a preferred embodiment of the anti-aging composition described in the present application, the preparation method of the Millettia pinnata seed extract comprises the following steps:

[0018] 1) adding greyhairy bean seed powder into enzymatic hydrolysis solution to carry out enzymatic hydrolysis reaction, freeze-drying to obtain a reaction product;

[0019] 2) extracting the reaction product by supercritical CO2 to obtain greyhairy bean seed extract;

[0020] The extraction conditions by supercritical CO2 include: extraction pressure of 30-35 Mpa, extraction temperature of 50-60℃, extraction time of 1.5-3h, separation pressure of 5.5-6.5 Mpa, separation temperature of 35-45℃ and CO2 flow rate of 15-30 L / h.

[0021] The present application adopts enzymatic pretreatment combined with supercritical CO2 extraction method, under the premise of retaining the activity of greyhairy bean seed polysaccharide and flavonoid glycoside, through accurate cracking of cell wall, the dissolution rate of target components is improved, at the same time, by using the inertness and low temperature characteristics of supercritical CO2, the green extraction process of no organic solvent residue and low allergenicity is realized, which is significantly better than the traditional organic solvent or high temperature water extraction method, especially in line with the high purity and safety demand of skin care raw materials.

[0022] The greyhairy bean seed extract prepared by the above method is richer in polysaccharide and flavonoid glycoside compounds compared with other methods, and forms an anti-aging composition with the remaining three components, which can reduce the level of stress hormone cortisol when applied to skin care, block the collagen degradation and barrier damage induced by stress hormone from the source, and relieve skin stress.

[0023] Among them, the greyhairy bean seed extract and ascorbic acid polypeptide synergistically improve cell vitality and improve the problems of dull, rough, loose and fine lines caused by staying up late, work and emotional stress.

[0024] In some specific embodiments, the greyhairy bean seeds are taken out after peeling the greyhairy bean, dried, crushed, and sieved to obtain greyhairy bean seed powder.

[0025] As a preferred embodiment of the anti-aging composition described in the present application, the weight ratio of greyhairy bean seed powder to enzymatic hydrolysis solution in step 1) is 1:(5-15).

[0026] The above weight ratio range of greyhairy bean seed powder and enzymatic hydrolysis solution can obtain more polysaccharide and flavonoid glycoside compounds, and improve the dissolution rate of target components.

[0027] More preferably, the weight ratio of greyhairy bean seed powder to enzymatic hydrolysis solution in step 1) is 1:(8-12).

[0028] The greyhairy bean seed powder and enzymatic hydrolysis solution preferably have the above weight ratio, and the obtained greyhairy bean seed extract has better quality.

[0029] As a preferred embodiment of the anti-aging composition described in the application, in the step 1), the enzymatic solution comprises 2.5-5 million U / L of cellulase and 0.8-1.2 million U / L of hemicellulase, and the pH value of the enzymatic solution is 5.0-5.5.

[0030] As a preferred embodiment of the anti-aging composition described in the application, in the step 1), the enzymatic solution comprises 2.5-5 million U / L of cellulase and 0.8-1.2 million U / L of hemicellulase, and the pH value of the enzymatic solution is 5.0-5.5.

[0031] In the technical solution of the application, the enzymatic solution contains the cellulase and the hemicellulase described above, and the greyhairspea seed powder is subjected to enzymolysis, and the supercritical CO2 method is used to obtain more polysaccharides and flavone glycosides, so as to better improve the skin hydration, form a hydrophilic gel layer to improve the stratum corneum hydration, reduce the diffusion resistance of hydrophilic compounds, and also promote the penetration of μ-conotoxin and ascorbic acid polypeptide, thereby improving the anti-aging effect of the composition.

[0032] Through the above technical solution, the polyphenolic compounds in the camellia nitidissima chung extract interfere with the lipid arrangement of skin cells, enhance the lipid fluidity, and promote the penetration of lipophilic ingredients; the polysaccharides in the greyhairspea seed extract improve the skin hydration, form a hydrophilic gel layer to improve the stratum corneum hydration, and reduce the diffusion resistance of hydrophilic compounds; the camellia nitidissima chung extract and the greyhairspea seed extract have a synergistic penetration-promoting effect, the flavonoids in the camellia nitidissima chung extract and the greyhairspea seed extract affect the expression of tight junction proteins, expand the intercellular tight junction, and assist the penetration of macromolecular peptides, thereby improving the utilization rate of μ-conotoxin and ascorbic acid polypeptide, and further exerting the corresponding anti-aging effect.

[0033] The application also provides an application of the anti-aging composition described above in the preparation of a product for reducing skin cell stress damage.

[0034] The anti-aging composition provided by the application can reduce skin cell stress damage and improve cell vitality, thereby improving the problems of skin dullness, roughness, sagging, and fine lines caused by staying up late, work, and emotional stress, and is mild and non-irritating, and is suitable for use by people with sensitive skin.

[0035] The application also provides an application of the anti-aging composition described above in the preparation of a product for lightening, anti-aging, repair, or anti-sensitivity skin care.

[0036] Preferably, the skin care product comprises at least one of the following: cosmetic water, emulsion, cream, mask, serum, and spray.

[0037] The application also provides an anti-aging skin care product, which comprises an emulsion.

[0038] The emulsion comprises the following components in the following mass percentages:

[0039] Thickener 0.05-0.3%, moisturizer 0.5-3%, pH adjuster 0.01-0.3%, emulsifier 0.5-5%, preservative 0.5-3%, anti-aging composition 1-5%.

[0040] As a preferred embodiment of the anti-aging skin care product described in the present application, the thickener includes at least one of polyacrylate crosspolymer-6, carbomer, carrageenan, gellan gum, xanthan gum, microcrystalline cellulose, cellulose gum, ethyl cellulose, tara gum, guar gum, ammonium acryloyldimethyltaurate / VP copolymer, acrylic acid (ester) copolymer, and sclerotium gum;

[0041] The moisturizing agent includes at least one of glycerin, D-panthenol, vitamin B5, 1,2-butylene glycol, 1,3-butylene glycol, 1,2-hexanediol, 1,3-propylene glycol, caprylyl glycol, dipropylene glycol, tremella polysaccharide, trehalose, betaine, allantoin, sodium hyaluronate, β-glucan, budding pullulan polysaccharide, and ceramide;

[0042] The pH regulator includes at least one of arginine, tromethamine, and EDTA-disodium;

[0043] The emulsifier includes at least one of caprylic / capric triglyceride, C14-22 alcohol, C12-20 alkyl glucoside, sodium polyacrylate, hydrogenated lecithin, cetearyl glucoside, isononyl isononanoate, pentaerythritol tetraisostearate, polydimethylsiloxane, stearyl alcohol, hydroxystearic acid, polymethylsilsesquioxane, pentaerythritol distearate, and sucrose stearate;

[0044] The preservative includes at least one of p-hydroxyacetophenone and polyol.

[0045] Through the above technical solutions, the addition of the above types of thickeners can better adjust the viscosity of the product, make the texture of the skin care product more stable, enhance the product consistency and improve the touch.

[0046] Adding the above types of moisturizers can absorb and retain moisture, reduce moisture loss in the skin, and provide long-lasting moisturizing, thereby improving the softness and elasticity of the skin. In addition, it can also reduce the appearance of fine lines and wrinkles.

[0047] Adding the above types of pH adjusters can maintain the acid-base balance of the product, ensure the stability of the formula and avoid irritation to the skin; adding the above types of emulsifiers can promote the mixing of oil and water, forming a stable emulsion structure, making cosmetics easier to apply and with a uniform texture.

[0048] Compared with the prior art, this application has the following beneficial effects:

[0049] The application provides an anti-aging composition for reducing stress fatigue, and a preparation method and application thereof. The anti-aging composition contains camellia nitidissima hu extract, albizia kalkora seed extract, mu-conotoxin and ascorbic acid polypeptide. A multi-target synergistic pathway is creatively constructed. The flavonoid glycosides in the albizia kalkora seed extract directly down-regulate the cortisol level, thereby blocking the collagen degradation and barrier damage induced by stress hormones from the source. Meanwhile, the camellia nitidissima hu extract and the ascorbic acid polypeptide form a double-antioxidant matrix, which synergistically neutralizes the reactive oxygen species (ROS) triggered by overwork and emotional stress, and promotes collagen neogenesis. The mu-conotoxin precisely inhibits the calcium ion channel of motor neurons, thereby relieving the dynamic wrinkle fixation caused by the continuous contraction of expression muscles. The four components form a cascade anti-aging network of “stress blocking-oxidation scavenging-collagen remodeling-muscle relaxation”. In addition, the polyphenol components in the camellia nitidissima hu extract enhance the lipid fluidity of the cell membrane, the hydration gel layer formed by the polysaccharides in the albizia kalkora seed extract improves the permeability of the stratum corneum, and the flavonoid components in the two components synergistically regulate the expression of tight junction proteins to expand the intercellular space, thereby significantly optimizing the transdermal efficiency of the active ingredients such as mu-conotoxin and ascorbic acid polypeptide. The anti-aging composition provided by the application can reduce the stress damage of skin cells and improve the cell vitality, thereby improving the problems of dullness, roughness, sagging and fine lines caused by overwork, work and emotional stress, and is mild and non-irritating, and is suitable for people with sensitive skin. BRIEF DESCRIPTION OF DRAWINGS

[0050] Figure 1 The results of improving gloss and skin texture before and 28 days after application of application example 7 and blank application example. DETAILED DESCRIPTION

[0051] For the purpose of better illustrating the application, technical solutions and advantages, the application will be further described in conjunction with the drawings and specific examples.

[0052] In the following examples and comparative examples, the experimental methods used are conventional methods unless otherwise specified, and the materials, reagents, etc. used are commercially available unless otherwise specified, and the component raw materials used in each parallel experiment are the same.

[0053] The raw materials used in the examples and comparative examples in the application are commercially available unless otherwise specified, and specifically are as follows:

[0054] Mu-conotoxin: purchased from Shenzhen Weiqi Biological Technology Co., Ltd.;

[0055] Ascorbic acid polypeptide: purchased from BioHarvest Sciences Co., Ltd. in Canada;

[0056] Camellia nitidissima hu extract: purchased from Ximu Source (Xiamen) Biological Technology Co., Ltd.;

[0057] Honeysuckle extract: purchased from Ningke (Xi'an) Biomedicine Technology Co., Ltd.;

[0058] Soybean extract: purchased from Shanxi Kangruikejian Biotechnology Co., Ltd.;

[0059] Millettia pachycarpa seed extract 1, self-made, including the following steps:

[0060] 1) After peeling the Millettia pachycarpa, the Millettia pachycarpa seeds were taken out, dried, crushed, sieved, and the Millettia pachycarpa seed powder was obtained, which was added into an enzymatic hydrolysis solution (the enzymatic hydrolysis solution included 3.75 million U / L cellulase and 1 million U / L hemicellulase, and the pH value of the enzymatic hydrolysis solution was 5.2) to perform an enzymatic hydrolysis reaction, the weight ratio of the Millettia pachycarpa seed powder to the enzymatic hydrolysis solution was 1:10, the enzymatic hydrolysis temperature was 50°C, the enzymatic hydrolysis time was 1.5 hours, and freeze-drying was performed to obtain a reaction product;

[0061] 2) The reaction product was extracted by supercritical CO2 to obtain the Millettia pachycarpa seed extract;

[0062] The extraction conditions by supercritical CO2 included an extraction pressure of 33 Mpa, an extraction temperature of 55°C, an extraction time of 2 hours, a separation pressure of 6.0 Mpa, a separation temperature of 40°C, and a CO2 flow rate of 25 L / h.

[0063] Millettia pachycarpa seed extract 2, self-made, including the following steps:

[0064] 1) After peeling the Millettia pachycarpa, the Millettia pachycarpa seeds were taken out, dried, crushed, sieved, and the Millettia pachycarpa seed powder was obtained, which was added into an enzymatic hydrolysis solution (the enzymatic hydrolysis solution included 2.5 million U / L cellulase and 0.8 million U / L hemicellulase, and the pH value of the enzymatic hydrolysis solution was 5) to perform an enzymatic hydrolysis reaction, the weight ratio of the Millettia pachycarpa seed powder to the enzymatic hydrolysis solution was 1:5, the enzymatic hydrolysis temperature was 45°C, the enzymatic hydrolysis time was 1 hour, and freeze-drying was performed to obtain a reaction product;

[0065] 2) The reaction product was extracted by supercritical CO2 to obtain the Millettia pachycarpa seed extract;

[0066] The extraction conditions by supercritical CO2 included an extraction pressure of 30 Mpa, an extraction temperature of 50°C, an extraction time of 1.5 hours, a separation pressure of 5.5 Mpa, a separation temperature of 35°C, and a CO2 flow rate of 15 L / h.

[0067] Millettia pachycarpa seed extract 3, self-made, including the following steps:

[0068] 1) peeling gray soybeans, removing gray soybean seeds, drying, crushing, and sieving to obtain gray soybean seed powder, adding an enzymatic hydrolysis solution (the enzymatic hydrolysis solution comprises 5 million U / L cellulase and 1.2 million U / L hemicellulase, and the enzymatic hydrolysis solution has a pH value of 5.5) to perform an enzymatic hydrolysis reaction, wherein the weight ratio of the gray soybean seed powder to the enzymatic hydrolysis solution is 1:15, the enzymatic hydrolysis temperature is 55° C., the enzymatic hydrolysis time is 5 hours, and freeze-drying is performed to obtain a reactant;

[0069] 2) extracting the reactants by supercritical CO2 to obtain a soybean seed extract;

[0070] The conditions for supercritical CO2 extraction include: extraction pressure of 35 MPa, extraction temperature of 60°C, extraction time of 3 h, separation pressure of 6.5 MPa, separation temperature of 45°C and CO2 flow rate of 30 L / h.

[0071] Ganoderma lucidum seed extract 4, homemade, including the following steps:

[0072] 1) peeling gray soybeans, removing gray soybean seeds, drying, crushing, and sieving to obtain gray soybean seed powder, adding an enzymatic hydrolysis solution (the enzymatic hydrolysis solution comprises 3.75 million U / L cellulase and 1 million U / L hemicellulase, and the enzymatic hydrolysis solution has a pH value of 5.2) to perform an enzymatic hydrolysis reaction, wherein the weight ratio of the gray soybean seed powder to the enzymatic hydrolysis solution is 1:8, the enzymatic hydrolysis temperature is 50° C., the enzymatic hydrolysis time is 1.5 hours, and freeze-drying is performed to obtain a reactant;

[0073] 2) extracting the reactants by supercritical CO2 to obtain a soybean seed extract;

[0074] The conditions for supercritical CO2 extraction include: extraction pressure of 33 MPa, extraction temperature of 55°C, extraction time of 2 h, separation pressure of 6.0 MPa, separation temperature of 40°C and CO2 flow rate of 25 L / h.

[0075] Ganoderma lucidum seed extract 5, homemade, including the following steps:

[0076] 1) peeling gray soybeans, removing gray soybean seeds, drying, crushing, and sieving to obtain gray soybean seed powder, adding an enzymatic hydrolysis solution (the enzymatic hydrolysis solution comprises 3.75 million U / L cellulase and 1 million U / L hemicellulase, and the enzymatic hydrolysis solution has a pH value of 5.2) to perform an enzymatic hydrolysis reaction, wherein the weight ratio of the gray soybean seed powder to the enzymatic hydrolysis solution is 1:12, the enzymatic hydrolysis temperature is 50° C., the enzymatic hydrolysis time is 1.5 hours, and freeze-drying is performed to obtain a reactant;

[0077] 2) extracting the reactants by supercritical CO2 to obtain a soybean seed extract;

[0078] The conditions for extraction by supercritical CO2 include: extraction pressure of 33 Mpa, extraction temperature of 55℃, extraction time of 2h, separation pressure of 6.0 Mpa, separation temperature of 40℃ and CO2 flow rate of 25 L / h.

[0079] Greyhairy bean seed extract 6, self-made, including the following steps:

[0080] 1) After peeling the greyhairy bean, the greyhairy bean seeds were taken out, dried, crushed, sieved to obtain greyhairy bean seed powder, and then added into an enzymatic hydrolysis solution (the enzymatic hydrolysis solution included 3.75 million U / L of cellulase and 1 million U / L of hemicellulase, and the pH value of the enzymatic hydrolysis solution was 5.2) to perform an enzymatic hydrolysis reaction, the weight ratio of the greyhairy bean seed powder to the enzymatic hydrolysis solution was 1:10, the enzymatic hydrolysis temperature was 50℃, the enzymatic hydrolysis time was 1.5 hours, and then freeze-drying was performed to obtain a reaction product;

[0081] 2) The reaction product was extracted by supercritical CO2 to obtain the greyhairy bean seed extract;

[0082] The conditions for extraction by supercritical CO2 include: extraction pressure of 33 Mpa, extraction temperature of 55℃, extraction time of 2h, separation pressure of 6.0 Mpa, separation temperature of 40℃ and CO2 flow rate of 25 L / h.

[0083] Greyhairy bean seed extract 7, self-made, including the following steps:

[0084] After peeling the greyhairy bean, the greyhairy bean seeds were taken out, dried, crushed, sieved to obtain greyhairy bean seed powder, and then extracted by supercritical CO2 to obtain the greyhairy bean seed extract;

[0085] The conditions for extraction by supercritical CO2 include: extraction pressure of 33 Mpa, extraction temperature of 55℃, extraction time of 2h, separation pressure of 6.0 Mpa, separation temperature of 40℃ and CO2 flow rate of 25 L / h.

[0086] The greyhairy bean seed extract 7 does not have an enzymatic hydrolysis process compared with the greyhairy bean seed extract 1.

[0087] Greyhairy bean seed extract 8, self-made, including the following steps:

[0088] 1) After peeling the greyhairy bean, the greyhairy bean seeds were taken out, dried, crushed, sieved to obtain greyhairy bean seed powder, and then added into an enzymatic hydrolysis solution (the enzymatic hydrolysis solution included 3.75 million U / L of pectinase and 1 million U / L of papain, and the pH value of the enzymatic hydrolysis solution was 5.2) to perform an enzymatic hydrolysis reaction, the weight ratio of the greyhairy bean seed powder to the enzymatic hydrolysis solution was 1:10, the enzymatic hydrolysis temperature was 50℃, the enzymatic hydrolysis time was 1.5 hours, and then freeze-drying was performed to obtain a reaction product;

[0089] 2) The reaction product was extracted by supercritical CO2 to obtain the greyhairy bean seed extract;

[0090] The conditions for supercritical CO2 extraction include: extraction pressure of 33 Mpa, extraction temperature of 55℃, extraction time of 2h, separation pressure of 6.0 Mpa, separation temperature of 40℃ and CO2 flow rate of 25 L / h.

[0091] The greyhairy milkvetch seed extract 9 is self-made, including the following steps:

[0092] The greyhairy milkvetch seed is taken out after peeling, dried, crushed, sieved, and then added into an ethanol solution (20% of ethanol by mass fraction) for ultrasonic extraction. The ultrasonic extraction is performed at a temperature of 50℃ for 30 min at a power of 120 W, and the solid-liquid ratio is 1 g:10 mL. After the ultrasonic extraction, the supernatant is collected by centrifugation, and then dried to obtain the greyhairy milkvetch seed extract.

[0093] Examples 1 to 15 and Comparative Examples 1 to 9, an anti-aging composition

[0094] Examples 1-15 and Comparative Examples 1-9 provide an anti-aging composition, and the formula is shown in Table 1 (parts by weight).

[0095] Table 1

[0096]

[0097]

[0098]

[0099] Comparative Example 10

[0100] Comparative Example 10 is different from Example 7 in that honeysuckle extract is used instead of Lonicera japonica extract, and the other components, parts and Example 7 are the same.

[0101] Comparative Example 11

[0102] Comparative Example 11 is different from Example 7 in that soybean extract is used instead of greyhairy milkvetch seed extract, and the other components, parts and Example 7 are the same.

[0103] Application Examples 1 to 17 and Comparative Application Examples 1 to 11

[0104] Application Examples 1-17 and Comparative Application Examples 1-11 provide an anti-aging emulsion, each raw material component and the mass percentage of each component are shown in Table 2. Although the anti-aging composition of the corresponding example / contrast example is described in Table 2, in order to avoid ambiguity, it is recommended to illustrate here, for example: Application Example 1 uses the anti-aging composition of Example 1, Application Example 2 uses the anti-aging composition of Example 2, and so on. Comparative Application Example 1 uses the anti-aging composition of Comparative Example 1, Comparative Application Example 2 uses the anti-aging composition of Comparative Example 2, and so on. Application Examples 16-17 use the anti-aging composition of Example 7.

[0105] Table 2

[0106]

[0107] The present application provides a preparation method of an anti-aging emulsion, the preparation method comprising the following steps:

[0108] (1) Mix the humectant, thickening agent and pH adjuster with water, stir, heat to 85°C, then homogenize at a speed of 1300 rpm for 4 min, after homogenization, keep warm for standby, obtain pre-preparation A component;

[0109] (2) Mix the emulsifier, heat to 75°C, then homogenize at a speed of 1300 rpm for 4 min, after homogenization, keep warm for standby, obtain pre-preparation B component;

[0110] (3) Mix the preservative, heat to 60°C to melt, obtain pre-preparation C component;

[0111] (4) Warm the pre-preparation A component to 80°C, add the pre-preparation B component at a speed of 300 rpm, stir and mix, then cool to 60°C, add the pre-preparation C component at a speed of 300 rpm, stir and mix, then cool to below 45°C, add the anti-aging composition, continue to stir for 8 min, finally add the remaining pH adjuster (arginine) to adjust the pH to 5.5-7.0, stop stirring, discharge, obtain the anti-aging emulsion.

[0112] Test Example 1, skin permeability test of the anti-aging composition

[0113] Experimental method: in vitro pig skin test, the exposed skin area in the diffusion cell is 1.5 cm 2 , the receiving chamber volume is 5.0 mL. Use a phosphate buffer solution with a pH of 5.8 and anhydrous ethanol with a volume ratio of 50:50 as the receiving liquid. Take 200 μL of the anti-aging emulsion prepared in Application Examples 1-17 and Comparative Application Examples 1-11, evenly apply it on the skin, and cover it with a film to prevent evaporation.

[0114] The initial total peptide content M0 in the emulsion and the total peptide transdermal absorption amount M1 after 24h were detected by BCA method, and the peptide transdermal rate was calculated as M1 / M2. The specific detection method was as follows: 50 μL sample was added into 1 mL BCA working solution, and reacted at 37°C for 30 min. The ultraviolet spectrophotometer was used to detect the absorbance OD value at 562 nm, and the total peptide content of the sample was calculated by comparing with the standard curve of bovine serum albumin (BSA). The greater the 24h peptide transdermal rate, the better the skin permeability of the anti-aging composition. The results are shown in Table 3 below.

[0115] Table 3

[0116]

[0117]

[0118] Conclusion: According to the above experiments, the 24h peptide transdermal rate of the anti-aging emulsion prepared in application examples 1-17 is 48%-86.4%, among which, the skin permeability of the anti-aging composition in application examples 2 and 5 is better than that in application examples 1 and 6, indicating that when the mass ratio of Camellia nitidissima H.T. Chang extract, Millettia pachycarpa extract, μ-conotoxin and ascorbic acid polypeptide is (0.5-3):(0.05-0.5):(0.01-0.5):(0.1-2), the skin permeability can be improved, and the skin permeability of the anti-aging composition in application examples 3-4 is better than that in application examples 2 and 5, indicating that when the mass ratio of Camellia nitidissima H.T. Chang extract, Millettia pachycarpa extract, μ-conotoxin and ascorbic acid polypeptide is (1-2):(0.2-0.3):(0.1-0.2):(0.6-0.8), the skin permeability can be better improved, and the anti-aging composition in application example 7 has the best skin permeability.

[0119] The anti-aging emulsion in application examples 16-17 added different mass percentages of anti-aging composition, and there was a difference in skin permeability of the anti-aging emulsion, indicating that the amount of anti-aging composition added had a great influence on the skin permeability.

[0120] The 24h peptide transdermal rate of the anti-aging emulsion prepared in application examples 7-11 is 80.9%-86.4%, and the anti-aging emulsion in application examples 10-11 is better than that in application examples 8-9 in terms of skin permeability, indicating that the weight ratio of Millettia pachycarpa seed powder to enzyme solution in the preparation process of Millettia pachycarpa seed extract is 1:(8-12), which is better than the effect brought by the weight ratio of 1:(5-15).

[0121] Compared with application examples 7-11 and application examples 12-14, when the extraction pressure, extraction temperature and extraction time in the preparation process of the extract of Millettia pachynema Benth seeds in the anti-aging composition of application example 12 are not within the scope of the present application (the extraction pressure is 30-35 MPa, the extraction temperature is 50-60 DEG C, and the extraction time is 1.5-3 h), the preparation process of the extract of Millettia pachynema Benth seeds in the anti-aging composition of application example 13 does not contain enzymatic treatment, and the preparation process of the extract of Millettia pachynema Benth seeds in the anti-aging composition of application example 14 uses other types of enzymatic hydrolysate, application examples 7-11 obtain more polysaccharides and flavonoids from Millettia pachynema Benth seeds, and application examples 12-14 are not as good as application examples 7-9 in improving skin hydration, reducing the diffusion resistance of hydrophilic compounds, and promoting the penetration of polypeptides. Compared with application examples 7-9 and application example 15, when the extract of Millettia pachynema Benth seeds is prepared by other methods (ultrasonic extraction has low extraction efficiency), the skin permeability of the obtained anti-aging composition is poor.

[0122] Compared with application examples 1-3 without Camellia nitidissima Chang extract and / or Millettia pachynema Benth seed extract, and compared with application examples 10-11, other types of extracts are used to replace Camellia nitidissima Chang extract or Millettia pachynema Benth seed extract, and the 24h peptide transdermal rate is lower, and the skin permeability of the anti-aging composition is poor. It is shown that Camellia nitidissima Chang extract and Millettia pachynema Benth seed extract have a synergistic penetration effect. The polyphenolic compounds in Camellia nitidissima Chang extract enhance the lipid fluidity by interfering with the arrangement of skin cell lipids, and promote the penetration of lipophilic ingredients; the polysaccharides in Millettia pachynema Benth seed extract improve skin hydration, form a hydrophilic gel layer to improve the hydration of the stratum corneum, and reduce the diffusion resistance of hydrophilic compounds; the flavonoids in Camellia nitidissima Chang extract and Millettia pachynema Benth seed extract affect the expression of tight junction proteins, expand the intercellular tight junction, and assist the penetration of macromolecular peptides.

[0123] Compared with application examples 6-7, when the anti-aging composition is used in the same amount, the skin permeability of the anti-aging composition without Camellia nitidissima Chang extract or Millettia pachynema Benth seed extract is not as good as that of the anti-aging composition used in application example 7.

[0124] Compared with application examples 8-9, when the mass ratio of Camellia nitidissima Chang extract and Millettia pachynema Benth seed extract is not within the scope of the present application, the skin permeability of the anti-aging composition is poor.

[0125] Test Example 2, effect of the anti-aging composition on skin cells under the state of overwork / fatigue stress

[0126] Sleep deprivation / fatigue stress accelerates skin aging through a dual mechanism of inducing oxidative stress and elevated cortisol levels. Under the condition of continuous sleep deprivation, a large amount of reactive oxygen species (ROS) accumulates in the body, directly attacking the mitochondrial function of dermal fibroblasts, leading to a significant decrease in cell viability; at the same time, stress activates the hypothalamic-pituitary-adrenal axis, causing abnormal increase in cortisol secretion. Cortisol, by binding to glucocorticoid receptors, inhibits collagen production and activates matrix metalloproteinases (MMPs) to accelerate collagen degradation. The synergistic effect of oxidative damage and cortisol forms a "double blow to cell function", causing a decrease in skin cell viability, a decrease in collagen, and ultimately leading to the destruction of the dermal matrix structure, loss of skin elasticity, and deepening of wrinkles and other signs of aging.

[0127] The cell line used in this test example is human dermal fibroblasts HDF (catalog number PC-202h, Wuhan Sino Biological Technology Co., Ltd.), and the test conditions are: incubator temperature 37±1℃, humidity 90±5%, carbon dioxide 5±1%; according to the grouping, the cells are cultured and treated, and then tested, the specific tests are cell activity test and type I collagen content detection, the test methods are as follows:

[0128] (1) The cell suspension was inoculated in a 96-well cell culture plate, with a density of 2000 cells per well, and 100 μL of DMEM high glucose culture medium containing 10% fetal bovine serum (Gibco) was added to each well, and cultured for 24 h;

[0129] (2) Discard the supernatant, add 100 μL of DMEM medium (2105341, Gibco) to the blank control group, add 100 μL of DMEM medium containing 200 μM hydrogen peroxide (Macklin, H792073) and 150 μM cortisol (Shanghai Yuanye Bio, S31360) to the model group, and add 100 μL of culture medium containing 0.5wt% corresponding anti-aging composition (anti-aging compositions prepared in Examples 1-15 and Comparative Examples 1-11) and 200 μM hydrogen peroxide and 150 μM cortisol to the sample group, with 6 holes repeated in each group, and cultured for 24 h;

[0130] (3) Cell activity determination: CCK8 cell proliferation kit (catalog number C6005M, Suzhou Youyilanbi Biological Technology Co., Ltd.) was used to detect the cell viability of each group, and the specific operation was to add 10 μL of CCK8 solution to each well, continue to incubate at 37℃ for 2 h, then use the enzyme marker to shake for 30 s, and use the enzyme-linked immunodetection instrument to detect the absorbance OD value at 450 nm wavelength;

[0131] (4) Type I collagen content detection: The type I collagen content of each group was detected using a human type I collagen (Col I) ELISA kit (item number ml057630, Shanghai ZhiLian Bioengineering). The cells of each group were collected, and the supernatant was discarded. The cells were lysed with RIPA lysis buffer (Shanghai Merck, V900854) containing protease inhibitors. The lysed sample was centrifuged at 12000g for 5 minutes, and the supernatant was taken. Then, according to the kit instructions, an equal amount of supernatant was added to the corresponding kit reaction wells. After incubation, washing, color development and other steps, the absorbance OD value was measured at a specific wavelength using an enzyme-labeled instrument. The type I collagen content of each group was calculated according to the standard curve.

[0132] The cell viability improvement effect of the composition was represented by the improvement of cell survival, and the anti-aging effect was represented by the improvement of type I collagen content. The calculation method was as follows:

[0133] Improvement rate = (OD value of sample - OD value of blank control) / OD value of blank control x 100%; 样品组 模型组 模型组 The results are shown in Table 4.

[0134] Table 4

[0135] Group Cell survival improvement rate / % Collagen type I content improvement rate / % Example 1 37.6 88.8 Example 2 41.3 117.4 Example 3 58.5 147.4 Example 4 58.5 144.9 Example 5 47.2 110.5 Example 6 38.9 103.7 Example 7 63.9 157.3 Example 8 59.1 152.7 Example 9 59.2 152.3 Example 10 61.6 154.9 Example 11 62.3 155.7 Example 12 48.7 143.7 Example 13 50.9 144.6 Example 14 54.1 146.3 Example 15 42.5 111.8 Comparative Example 1 22.4 14.8 Comparative Example 2 16.5 60.7 Comparative Example 3 10.5 11.1 Comparative Example 4 31.9 65.2 Comparative Example 5 11.2 43.5 Comparative Example 6 17.2 63.2 Comparative Example 7 23.0 17.9 Comparative Example 8 35.8 73.8 Comparative Example 9 32.0 66.3 Comparative Example 10 25.1 15.3 Comparative Example 11 8.4 66.2

[0136] Conclusion: The cell survival improvement rate of the anti-aging composition prepared in Examples 1-15 was 37.6%-63.9%, and the type I collagen content improvement rate was 88.8%-157.3%. This shows that the anti-aging composition of the application can better improve the cell viability effect and anti-aging effect.

[0137] Among them, the anti-aging composition of Examples 2 and 5 has better cell viability effect and anti-aging effect than Examples 1 and 6, which shows that when the mass ratio of Camellia nitidissima extract, Millettia pachycarpa seed extract, μ-conotoxin and ascorbic acid polypeptide is (0.5-3):(0.05-0.5):(0.01-0.5):(0.1-2), the cell viability effect and anti-aging effect can be improved. The anti-aging composition of Examples 3-4 has better cell viability effect and anti-aging effect than Examples 2 and 5, which shows that when the mass ratio of Camellia nitidissima extract, Millettia pachycarpa seed extract, μ-conotoxin and ascorbic acid polypeptide is (1-2):(0.2-0.3):(0.1-0.2):(0.6-0.8), the cell viability effect and anti-aging effect can be better improved. The anti-aging composition of Example 7 has the best cell viability effect and anti-aging effect.

[0138] ​​The cell survival improvement rate of the anti-aging composition prepared in Examples 7-11 is 59.1%-63.9%, and the type I collagen content improvement rate is 152.3%-157.3%. The anti-aging compositions of Examples 10-11 are superior to Examples 8-9 in improving cell vitality and anti-aging effect, indicating that the weight ratio of the Millettia pachycarpa seed powder to the enzymatic hydrolysate in the preparation process of the Millettia pachycarpa seed extract is 1:(8-12), which is superior to the effect brought by the weight ratio of 1:(5-15).

[0139] Compared with Examples 7-11 and Examples 12-14, when the extraction pressure, extraction temperature and extraction time in the preparation process of the Millettia pachycarpa seed extract in the anti-aging composition of Example 12 are not within the scope of the present application (extraction pressure is 30-35 MPa, extraction temperature is 50-60°C, and extraction time is 1.5-3h), the preparation process of the Millettia pachycarpa seed extract in the anti-aging composition of Example 13 does not contain enzymatic treatment, and the preparation process of the Millettia pachycarpa seed extract in the anti-aging composition of Example 14 uses other types of enzymatic hydrolysate, the cell survival improvement rate and the type I collagen content improvement rate of Examples 12-14 are not as good as Examples 7-11.

[0140] When the Millettia pachycarpa seed extract of Example 15 is not extracted by the supercritical CO2 method, the cell survival improvement rate and the type I collagen content improvement rate of the obtained anti-aging composition are not as good as Examples 7-11.

[0141] The anti-aging compositions of Comparative Examples 1-3 do not contain Camellia nitidissima extract and / or Millettia pachycarpa seed extract, and Comparative Examples 10-11 use other types of extracts to replace Camellia nitidissima extract or Millettia pachycarpa seed extract, and the cell survival improvement rate and the type I collagen content improvement rate of the anti-aging compositions are not as good as Example 7, indicating that Camellia nitidissima extract and Millettia pachycarpa seed extract can synergistically improve cell vitality and anti-aging effect.

[0142] Comparative Examples 6-7 remove the Millettia pachycarpa seed extract alone while keeping the amount of the anti-aging composition unchanged, and the cell vitality effect of the anti-aging composition is not as good as the anti-aging composition used in Example 7; and the anti-aging composition removes the Camellia nitidissima extract alone, and the anti-aging effect is not as good as the anti-aging composition used in Example 7.

[0143] The mass ratio of Camellia nitidissima extract to Millettia pachycarpa seed extract in Comparative Examples 8-9 is not within the scope of the present application, and the cell vitality effect and anti-aging effect of the anti-aging composition are not as good as the anti-aging composition used in Example 7.

[0144] Test Example 3, human skin patch test

[0145] Sixty volunteers, 30 males and 30 females, aged 20-50 years, were divided into two groups according to a random number table method, 15 males and 15 females in each group. A closed patch test method was used, and equal amounts (0.020 mL-0.025 mL) of test samples (emulsions prepared by application examples 1-17, comparative application examples 1-11, and blank application example) were placed in a specific patch tester, which was attached to the arm of the volunteer with a low-sensitivity adhesive tape, and was lightly pressed to make it evenly attached to the skin, and was left for 24 h; the blank control group was distilled water, and the blank application example was an emulsion without an anti-aging composition. Each volunteer had one patch tester on each arm, and each patch tester had 10 holes, so each volunteer (two hands) could test 20 samples at the same time. The test samples of the first group were emulsions prepared by application examples 1-17 and blank application example, and the test samples of the second group were emulsions prepared by comparative application examples 1-11 and blank application example, and a blank control was set up for each group. After 24 h, the patch tester was removed, and the skin reaction was observed after 0.5 h, 24 h, and 48 h, and the results were recorded. The adverse reaction grade of the skin is shown in Table 5 below.

[0146] Table 5

[0147]

[0148] After testing, the anti-aging emulsions prepared by application examples 1-17 and comparative application examples 1-11 of the present application were all negative reactions after human patch testing, and were safe and non-irritating to human skin.

[0149] Test Example 4, efficacy test of the anti-aging composition on skin lightening, fine, firmness, and wrinkle reduction

[0150] Experimental method:

[0151] 1. Experimental basis:

[0152] Refer to the human efficacy evaluation test method in the "Cosmetic Safety Technical Specification" (2015 edition).

[0153] 2. Subject screening:

[0154] Inclusion criteria: recruit 25-40-year-old Asian adult volunteers who meet the following conditions:

[0155] a) Frequent late-night sleep (sleep after 11 p.m., and average daily sleep time less than 6 hours);

[0156] b) High work pressure (medical staff or engaged in emerging internet industry, daily work time greater than or equal to 9 hours);

[0157] c) Self-reported history of skin sensitivity (confirmed by dermatologist assessment);

[0158] d) No history of serious systemic disease or skin disease.

[0159] Exclusion criteria: pregnant or lactating women, severe allergic constitution, participated in other clinical trials within 3 months.

[0160] Number of subjects: 174 qualified volunteers were included, divided into 29 groups of 6 people each according to the random number table method.

[0161] 3. Sample application method:

[0162] Test samples: Anti-aging emulsions prepared in Examples 1-17, Comparative Examples 1-11, and Blank Example (double-blind code).

[0163] Usage: Volunteers applied 1 mL of the sample evenly to the entire face after cleansing in the morning and evening, and gently massaged until completely absorbed.

[0164] 4. Test period and process:

[0165] Test period: 28 days (D0 to D28).

[0166] Visit time points: Instrument testing was performed at D0 (baseline) and D28 (end of trial).

[0167] Preparation before testing:

[0168] a) After the subject arrived, the face was cleaned with a unified non-irritating cleansing product;

[0169] b) Rest for 30 minutes in a constant temperature and humidity environment (temperature 21±1℃, humidity 50±10%);

[0170] c) Maintain a closed-eye relaxed state during the test period to avoid expression movements that interfere with the test.

[0171] 5. Test instruments and detection indicators:

[0172] Before testing, the instrument was calibrated to the standard parameters. The researcher used the CK skin glossiness test probe GL200 to measure the glossiness of the cheekbones; the Cutometer elasticity tester to measure the tightness F4 of the cheekbones; and the VISIA-CR to take pictures and analyze the skin texture and wrinkles of the volunteers. During the test, the probe pressure and measurement time were fixed, and the same part was measured 3 times, with the average value taken.

[0173] The brightening effect of the sample was represented by the improvement in glossiness, the skin tightening effect was represented by the improvement in tightness F4 value, the skin fine effect was represented by the improvement in skin texture score, and the fine line lightening effect was represented by the improvement in the number of facial fine lines, with the formula as follows:

[0174] Glossiness value improvement rate = (glossiness T28 - glossiness T0Glossiness T0 × 100 %;

[0175] Improvement rate of firmness F4 value = (F4 T0 - F4 T28 ) / F4 T0 × 100 %;

[0176] Improvement rate of skin texture score = (skin texture score T0 - skin texture score T28 ) / skin texture score T0 × 100 %;

[0177] Improvement rate of fine line number = (fine line number T0 - fine line number T28 ) / fine line number T0 × 100 %;

[0178] The results are shown in Table 6.

[0179] Table 6

[0180]

[0181]

[0182] It is found by experiments that the improvement rate of glossiness value of the anti-aging emulsion prepared in application examples 1-17 is 7.9%-16.9%; the improvement rate of firmness F4 value is 15.8%-33.2%; the improvement rate of skin texture score is 33.9%-63.1%; and the improvement rate of fine line number is 28.7%-55.7%.

[0183] Among them, the improvement rate of glossiness value of the anti-aging emulsion prepared in application examples 1-7 is 7.9%-16.9%, the improvement rate of firmness F4 value is 15.8%-33.2%, the improvement rate of skin texture score is 33.9%-63.1%, and the improvement rate of fine line number is 28.7%-55.7%; the anti-aging emulsion prepared in application examples 1-7 can better improve glossiness, firmness, skin texture and fine lines; the anti-aging emulsion of application examples 3-4 is superior to application examples 2, 5 in improving glossiness, firmness, skin texture and fine lines; the anti-aging emulsion of application examples 2, 5 is superior to application examples 1, 6 in improving glossiness, firmness, skin texture and fine lines; and the anti-aging emulsion of application example 7 is best in improving glossiness, firmness, skin texture and fine lines.

[0184] The anti-aging emulsion of application examples 16-17 adds different mass percentages of anti-aging composition, and there is a difference in improving glossiness, firmness, skin texture and fine lines, which shows that the amount of anti-aging composition added has a great influence on improving glossiness, firmness, skin texture and fine lines.

[0185] The anti-aging emulsions prepared in application examples 7-11 have better improvement rates of glossiness value, F4 value of firmness, skin texture score, and number of fine lines. The anti-aging emulsions of application examples 10-11 are superior to application examples 8-9 in terms of skin permeability, indicating that the weight ratio of grey hair seed powder to enzymatic liquid during the preparation of grey hair seed extract is 1:(8-12), which is superior to the effect of the weight ratio of grey hair seed powder to enzymatic liquid of 1:(5-15) in improving glossiness, firmness, skin texture, and fine lines.

[0186] Compared with application examples 7-11 and application examples 12-14, when the extraction pressure, extraction temperature, and extraction time used in the preparation of grey hair seed extract in the anti-aging composition of application example 12 are not within the scope of the present application (extraction pressure of 30-35 Mpa, extraction temperature of 50-60℃, and extraction time of 1.5-3h), the preparation process of grey hair seed extract in the anti-aging composition of application example 13 does not contain enzymatic treatment, and the preparation process of grey hair seed extract in the anti-aging composition of application example 14 uses other types of enzymatic products to obtain more grey hair seed polysaccharides and flavonoids, and application examples 12-14 are inferior to application examples 7-11 in improving glossiness, firmness, skin texture, and fine lines. Compared with application examples 7-11 and application example 15, when the grey hair seed extract is prepared by other methods, the obtained anti-aging composition has poor effects in improving glossiness, firmness, skin texture, and fine lines.

[0187] Compared with application examples 1-3 without camellia sinensis extract and / or grey hair seed extract, and compared with application examples 11-12, other types of extracts are used to replace camellia sinensis extract or grey hair seed extract, and the anti-aging emulsions prepared have lower improvement rates of glossiness value, F4 value of firmness, skin texture score, and number of fine lines, indicating that camellia sinensis extract and grey hair seed extract have a synergistic effect in improving glossiness, firmness, skin texture, and fine lines.

[0188] Compared with application example 4 without μ-conotoxin and application example 5 without ascorbic acid polypeptide, the anti-aging emulsion has a poorer effect in improving glossiness, firmness, skin texture, and fine lines than application examples 1-7. Among them, camellia sinensis extract synergizes with ascorbic acid polypeptide to better improve glossiness, and the addition of μ-conotoxin can better improve firmness; μ-conotoxin synergizes with ascorbic acid polypeptide to better improve the number of fine lines.

[0189] Compared with application examples 6-7, the anti-aging emulsion has a poorer effect in improving glossiness, firmness, skin texture, and fine lines than the anti-aging emulsion used in application example 7 when the amount of the anti-aging composition is kept unchanged and only camellia sinensis extract or grey hair seed extract is removed.

[0190] The mass ratio of the extract of golden camellia and the extract of greyhairsed bean seed in Comparative Examples 8-9 is not within the scope of the present application, and the anti-aging emulsion is poor in improving gloss, firmness, skin texture and fine lines.

[0191] The results of improving gloss and skin texture before and after using the emulsion of Application Example 7 and the blank application are shown in Table 2. Figure 1

[0192] Finally, it should be noted that the above examples are only used to illustrate the technical solutions of the present application and are not a limitation on the scope of protection of the present application. Although the present application has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present application can be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present application.​

Claims

1. An anti-aging composition, characterized in that The anti-aging composition comprises golden camellia extract, ginkgo biloba seed extract, μ-conopeptide and ascorbic acid polypeptide; The mass ratio of the camellia chrysantha extract, glycine max seed extract, μ-conopeptide and ascorbic acid polypeptide is (0.1-5): (0.01-2): (0.001-1): (0.01-5).

2. The anti-aging composition according to claim 1, wherein The mass ratio of the camellia chrysantha extract, glycine max seed extract, μ-conopeptide and ascorbic acid polypeptide is (0.5-3): (0.05-0.5): (0.01-0.5): (0.1-2).

3. The anti-aging composition according to claim 2, wherein The mass ratio of the camellia chrysantha extract, glycine max seed extract, μ-conopeptide and ascorbic acid polypeptide is (1-2): (0.2-0.3): (0.1-0.2): (0.6-0.8).

4. The anti-aging composition according to claim 1, wherein The preparation method of the gray edamame seed extract comprises the following steps: 1) adding edamame seed powder to an enzymatic hydrolysis solution for enzymatic hydrolysis, and freeze-drying to obtain a reactant; 2) extracting the reactants by supercritical CO2 to obtain a soybean seed extract; The conditions for supercritical CO2 extraction include: extraction pressure of 30-35 MPa, extraction temperature of 50-60°C, extraction time of 1.5-3 h, separation pressure of 5.5-6.5 MPa, separation temperature of 35-45°C and CO2 flow rate of 15-30 L / h.

5. The anti-aging composition according to claim 4, characterized in that In the step 1), the weight ratio of the gray soybean seed powder to the enzymatic hydrolyzate is 1:(5-15).

6. The anti-aging composition according to claim 5, characterized in that In the step 1), the weight ratio of the gray soybean seed powder to the enzymatic hydrolyzate is 1:(8-12).

7. The anti-aging composition according to claim 4, wherein In the step 1), the enzymatic hydrolysis solution comprises 2.5 to 5 million U / L of cellulase and 800,000 to 1.2 million U / L of hemicellulase, and the pH value of the enzymatic hydrolysis solution is 5.0 to 5.

5.

8. The anti-aging composition according to claim 4, wherein The enzymatic hydrolysis temperature in step 1) is 45-55° C., and the enzymatic hydrolysis time is 1-2 hours.

9. Use of the anti-aging composition according to any one of claims 1 to 8 in the preparation of brightening, anti-aging, repairing or anti-sensitive skin care products.

10. An anti-aging skin care product, characterized in that: The anti-aging skin care product includes an emulsion; the emulsion includes the following components in percentage by mass: Thickener 0.05-0.3%, moisturizer 0.5-3%, pH adjuster 0.01-0.3%, emulsifier 0.5-5%, preservative 0.5-3%, anti-aging composition 1-5%.

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