Skin-tendering and freckle-removing essence and preparation method thereof

Abstract

The invention relates to the field of daily chemical products, in particular to skin-tendering and freckle-removing essence and a preparation method thereof. The essence is prepared from the following raw materials in percentage by weight: 0.01 to 0.5 percent of alpha-arbutin, 0.01 to 0.5 percent of glabridin, 0.01 to 0.5 percent of asiaticoside, 0.2 to 1.5 percent of an uncaria chinensis leaf extract, 0.2 to 1.5 percent of a phyllanthus emblica leaf extract, 0.2 to 1.5 percent of a beetroot extract, 0.2 to 1.5 percent of a holy basil leaf extract, 5 to 20 percent of polyhydric alcohol, 1 to 3 percent of a solubilizer, 0 to 2 percent of auxiliary materials and the balance of water. Alpha-arbutin, glabridin and asiaticoside are adopted to endow the essence with the effects of whitening, removing freckles, moisturizing, tendering skin and the like, and meanwhile, a large amount of polyhydric alcohols are added to endow the skin with better moisturizing and tendering effects; meanwhile, active ingredients such as uncaria chinensis leaf extract, phyllanthus emblica leaf extract, beet root extract and ocimum shengbasil leaf extract are further added, so that the whitening and freckle removing effects of the essence can be remarkably improved, the updating effect of skin epidermal cells can be effectively improved, and the skin is endowed with a tender effect.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of daily chemical products, in particular to a skin-smoothing and freckle-removing essence and a preparation method thereof. BACKGROUND

[0002] The skin-smoothing and freckle-removing essence is a functional skin care product designed for rough skin and pigmentation, and has the dual functions of improving skin quality and lightening skin color, achieving the synergistic improvement of "fine skin quality + even skin color". The skin-smoothing effect needs to strengthen the skin barrier, promote cell renewal and improve the support of the dermis; by activating the proliferation of keratinocytes, accelerating the metabolism of aged keratin, improving rough skin and large pores, increasing skin fullness and elasticity, and making the touch more smooth and smooth. The freckle-removing effect needs to act on the whole chain of melanin generation, transport and deposition, inhibit tyrosinase activity (reduce melanin synthesis), block the transport of melanin particles to keratinocytes, and accelerate the metabolism of formed pigments, fade sunspots, acne marks, and yellow-brown spots, and achieve even and bright skin color.

[0003] Essences have various forms, including water-like, egg-white-like, oil-like, and gel-like forms. Water-like essence (such as hyaluronic acid essence) is refreshing and easy to absorb, suitable for oily and mixed oily skin; oil-like essence (such as rosehip oil essence) is high in moisturizing, suitable for dry skin or autumn and winter seasons; gel-like essence (such as aloe vera essence) has both moisturizing and soothing properties, and can be used by sensitive skin.

[0004] Invention patent CN119097567B discloses a whitening and freckle-removing essence and a preparation method thereof, which is formed by mixing additives such as niacinamide, EDTA, L-lactic acid, citric acid, and baicalin inclusion.

[0005] Invention patent CN115887322B discloses a whitening and freckle-removing, skin-smoothing and moisturizing essence and a preparation method thereof, which comprises Artemisia argyi extract, Morinda citrifolia extract, Ligusticum chuanxiong extract, white mulberry extract, cucumber extract, humectant, emollient, emulsifier and water.

[0006] Invention patent CN114796042B discloses a processing method of an anti-aging, freckle-removing, whitening and moisturizing essence, comprising the following steps: step one, washing raw materials, washing Chinese herbal medicine with anti-aging effect, then cutting into sections, and then using activated carbon for decolorization; step two, extracting ingredients, pouring Chinese herbal medicine into clean water; step three, preparing water phase, mixing Chinese herbal medicine extract, mulberry extract and mountain berry extract, then adding deionized water; step four, preparing oil phase; step five, forming, mixing water phase and oil phase according to the volume ratio of 1:3, then pouring into the emulsifying device for sufficient stirring.

[0007] The application patent CN115444800B discloses a whitening and freckle-removing essence and a preparation method thereof. The essence includes the following components in mass percentage: glycerol 2-4%, xanthan gum 0.08-0.12%, phenylethyl resorcinol 0.8-1.2%, perilla ketone 0.3-0.5%, skin conditioning agent 1-3%, skin tendering agent 6-8%, moisturizing aid 2.3-2.7%, antioxidant 0.4-0.6%, arginine 0.4-0.6%, disodium EDTA 0.03-0.05%, and water in the rest; wherein the skin conditioning agent includes citric acid and mushroom extract; the skin tendering agent includes hydrolyzed algal extract and zucchini seed extract.

[0008] However, for the skin tendering and freckle-removing essence, it is still a technical problem to be solved to improve the main performance of the essence in freckle removal and skin tendering. SUMMARY

[0009] In view of the above technical problems, the present application provides a skin tendering and freckle-removing essence and a preparation method thereof.

[0010] The skin tendering and freckle-removing essence provided by the present application has raw materials of alpha-arbutin, glabridin, asiaticoside, Uncaria sinensis leaf extract, Phyllanthus emblica leaf extract, Beta vulgaris root extract, Ocimum sanctum leaf extract, polyhydric alcohol, solubilizer, auxiliary material, and water.

[0011] The alpha-arbutin used in the present application is a natural whitening ingredient derived from plants. Its core mechanism of action is to inhibit the activity of tyrosinase and reduce the biosynthesis of melanin. Alpha-arbutin can competitively bind to tyrosinase, preventing it from catalyzing the conversion of tyrosine to dopa (a precursor of melanin), thereby reducing skin pigmentation from the source and improving problems such as color spots, freckles, and acne marks. In addition, the glycoside group in its molecular structure can enhance the hydration capacity of the stratum corneum, reduce dryness-induced roughness and desquamation, and make the skin feel softer. Glabriden is an active ingredient extracted from plants in the genus Glycyrrhiza. It has multiple functions such as whitening, anti-inflammatory, and antioxidant. It can inhibit the activity of tyrosinase and dopachrome tautomerase, block melanin production through two pathways, and accelerate the decomposition of existing melanin, thereby significantly improving stubborn color spots such as chloasma and sunspots. This material can also protect skin cells from oxidative damage, delay collagen loss, improve fine lines and skin laxity. It can also restore the normal shedding and renewal rhythm of the stratum corneum, metabolize aged keratin, and prevent skin roughness. Asiaticoside is the core active ingredient in the extract of Centella asiatica. It can stimulate the activity of skin fibroblasts, promote cell renewal and metabolism, promote the synthesis of collagen and hyaluronic acid, and accelerate the repair of the skin barrier, with good skin-smoothing effect. It can also enhance the water-retention capacity of the stratum corneum and improve dryness and desquamation.

[0012] The above-mentioned alpha-arbutin, glabriden, and asiaticoside can impart certain whitening, spot-removing, moisturizing, and skin-smoothing effects to the serum. The addition of a large amount of polyhydric alcohol can impart moisturizing and skin-smoothing effects to the skin. Meanwhile, the present application further adds active ingredients such as Uncaria hookeriana leaf extract, Phyllanthus emblica leaf extract, Beta vulgaris root extract, and Ocimum sanctum leaf extract, which can significantly improve the whitening and spot-removing effects of the serum and promote the renewal of epidermal cells, imparting a tender effect to the skin.

[0013] Preferably, the skin-smoothing and spot-removing serum of the present application contains at least one of a preservative, a thickening agent, a pigment, a fragrance, a pH adjuster, and a chelating agent.

[0014] Preferably, the skin-smoothing and spot-removing serum of the present application contains the following ingredients in the following proportions by weight: 0.01-0.5% alpha-arbutin, 0.01-0.5% glabriden, 0.01-0.5% asiaticoside, 0.2-1.5% Uncaria hookeriana leaf extract, 0.2-1.5% Phyllanthus emblica leaf extract, 0.2-1.5% Beta vulgaris root extract, 0.2-1.5% Ocimum sanctum leaf extract, 5-20% polyhydric alcohol, 1-3% solubilizer, 0-2% auxiliary material, and the balance water.

[0015] Preferably, the amount of auxiliary materials in the skin-whitening and freckle-removing essence of the present application is as follows: 0-0.3% preservative, 0-0.8% thickening agent, 0-0.05% pigment, 0-0.05% flavoring agent, 0-0.5% pH regulator, and 0-0.3% chelating agent.

[0016] Preferably, the polyhydric alcohol in the skin-whitening and freckle-removing essence of the present application includes at least one of sorbitol, ethylene glycol, polyethylene glycol, glycerol, propylene glycol, butylene glycol, hexylene glycol, and pentylene glycol.

[0017] Preferably, the solubilizing agent in the skin-whitening and freckle-removing essence of the present application includes at least one of Tween-20, Tween 60, and PEG-40 hydrogenated castor oil.

[0018] Preferably, the weight ratio of the Uncaria hirsuta leaf extract and the Phyllanthus emblica leaf extract in the skin-whitening and freckle-removing essence of the present application is 1:0.8-1.5.

[0019] Preferably, the Uncaria hirsuta leaf extract in the skin-whitening and freckle-removing essence of the present application is prepared by crushing Uncaria hirsuta leaves, adding 30-55wt% ethanol solution for heating and leaching, filtering, concentrating, and drying to obtain the Uncaria hirsuta leaf extract.

[0020] Preferably, the Phyllanthus emblica leaf extract in the skin-whitening and freckle-removing essence of the present application is prepared by crushing Phyllanthus emblica leaves, adding 25-50wt% ethanol solution for heating and leaching, filtering, concentrating, and drying to obtain the Phyllanthus emblica leaf extract.

[0021] Preferably, the weight ratio of the Beta vulgaris root extract and the Ocimum sanctum leaf extract in the skin-whitening and freckle-removing essence of the present application is 1:0.4-1.

[0022] Preferably, the Beta vulgaris root extract and the Ocimum sanctum leaf extract in the skin-whitening and freckle-removing essence of the present application are both prepared by leaching with 20-40wt% ethanol solution.

[0023] The present application also provides a preparation method of the skin-whitening and freckle-removing essence, which comprises the following steps: adding water into a reaction container and heating, opening the stirring, adding polyhydric alcohol and solubilizing agent, stirring, then adding α-arbutin, glabridin, and asiaticoside, continuing to stir, cooling, adding Uncaria hirsuta leaf extract, Phyllanthus emblica leaf extract, Beta vulgaris root extract, and Ocimum sanctum leaf extract, continuing to stir, standing and aging, sterilizing and packaging, to obtain the skin-whitening and freckle-removing essence.

[0024] Preferably, the heating is performed at 55-75℃, and the cooling is performed at 35-50℃.

[0025] The beneficial effects of the present application are: The present application uses alpha-arbutin, glabridin, asiaticoside, Uncaria sinensis leaf extract, Phyllanthus emblica leaf extract, Beta vulgaris root extract, Ocimum sanctum leaf extract, polyol, solubilizer, excipient and water to prepare the essence; wherein alpha-arbutin, glabridin and asiaticoside can endow the essence with whitening, freckle-removing, moisturizing and skin-tendering effects, and the addition of a large amount of polyol can endow the skin with better moisturizing and skin-tendering effects. Meanwhile, the present application further adds active ingredients such as Uncaria sinensis leaf extract, Phyllanthus emblica leaf extract, Beta vulgaris root extract and Ocimum sanctum leaf extract, which can significantly improve the whitening and freckle-removing effects of the essence, and effectively improve the epidermal cell renewal of the skin, endowing the skin with a tender effect.

[0026] The Uncaria sinensis leaf extract and the Phyllanthus emblica leaf extract prepared by the present application can effectively inhibit the activity of tyrosinase and the formation of melanin, and both can greatly improve the whitening and freckle-removing effects of cosmetics.

[0027] The Uncaria sinensis leaf extract and the Phyllanthus emblica leaf extract prepared by the present application have a stronger inhibitory effect on melanin and tyrosinase when the weight ratio is within the range of 1:0.2-8, which is stronger than the effect of Uncaria sinensis leaf extract or Phyllanthus emblica leaf extract alone. The two can produce synergistic effects to improve the whitening and freckle-removing effects. In addition, when the Uncaria sinensis leaf extract and the Phyllanthus emblica leaf extract are combined in a weight ratio of 1:0.8-1.5, the whitening and freckle-removing effects are better.

[0028] The essence prepared by the present application can effectively promote cell renewal and metabolism, and is rich in moisturizing active ingredients such as polyol, Beta vulgaris root extract and Ocimum sanctum leaf extract, which can effectively endow the skin with skin-tendering and plumpness.

[0029] The use of Beta vulgaris root extract and Ocimum sanctum leaf extract in the essence of the present application can effectively promote the renewal and metabolism of keratinocytes. When Beta vulgaris root extract and Ocimum sanctum leaf extract are used together, the effect is better than when only one of them is used. The combination of the two can synergistically improve the renewal rate and effect of keratinocytes, especially when the ratio of the two is within the range of 1:0.4-1. DETAILED DESCRIPTION

[0030] The specific embodiments of the present invention are described below to facilitate understanding of the present invention by those skilled in the art. However, it should be clear that the present invention is not limited to the scope of the specific embodiments. For those skilled in the art, as long as various changes are within the spirit and scope of the present invention as defined and determined by the appended claims, these changes are obvious, and all inventions and creations utilizing the concepts of the present invention are protected.

[0031] Example 1 Preparation of Uncaria sinensis leaf extract: Crush the Uncaria sinensis leaves, add a 40 wt% ethanol solution at a solid-liquid ratio of 1 g:20 mL, heat and extract at 55°C and 100 rpm for 4 hours, then filter through a 0.22 μm filter membrane, and concentrate the filtrate at 55°C and 0.0095 MPa to 13.2% by weight of the filtrate, and then freeze-dry at -45°C to a water content of 0.95 wt% to obtain the Uncaria sinensis leaf extract.

[0032] Preparation of Phyllanthus emblica leaf extract: crush the Phyllanthus emblica leaves, add 35wt% ethanol solution at a solid-liquid ratio of 1g:27mL, heat and extract at 55°C and 100 rpm for 4.5 hours, then filter through a 0.22μm filter membrane, concentrate the filtrate at 55°C and 0.0092MPa to 14.5% of the filtrate weight, and then freeze-dry at -45°C to a water content of 1.01wt% to obtain the Uncaria sinensis leaf extract.

[0033] The weight ratios in Table 1 were used to prepare test samples for performance testing.

[0034] Table 1: Sample composition

[0035] The samples in Table 1 were tested for their freckle removal effects by inhibiting tyrosinase and melanin synthesis in the body and characterizing the freckle removal effects.

[0036] 1. Inhibition of tyrosinase test in vivo: Materials: B16-F10 cells, culture medium (DMEM medium containing 10% fetal bovine serum), PBS, and microplate reader.

[0037] Experimental process: B16-F10 cells in the logarithmic growth phase were taken and the density was 1.5×10 6Each well was inoculated into a 6-well plate, and culture medium was added to 2 mL per well. The cells were cultured in a 37°C, 5% CO2 incubator for 24 hours, the supernatant was discarded, and the cells were rinsed twice with PBS. The cells were then divided into groups: a blank group and a sample group. In the sample group, the above samples 1-11 were diluted to a concentration of 55 μg / mL using serum-free DMEM medium and then added to the well plate rinsed with PBS, 2 mL per well. In the blank group, 2 mL of serum-free DMEM medium was added to each well, with 4 parallels per group. The cells were then cultured in a 37°C, 5% CO2 incubator for 48 hours. The supernatant was aspirated and the remaining material (including the cells) was collected in a centrifuge tube. The supernatant was discarded after centrifugation, 40 μL of 1 wt% Triton-X100 solution was added, the cells were frozen and lysed at -80°C for 30 minutes, and then thawed in a 37°C water bath. Then, 10 μL of 1 wt% L-dopa solution was added, and the cells were reacted in a 37°C water bath for 30 minutes. The absorbance at a wavelength of 450 nm was measured. The tyrosinase inhibition rate was calculated according to the absorbance of the test, where tyrosinase inhibition rate = (blank group OD - 1 mol / L - sample group OD / blank group OD). The test results are shown in Table 2.

[0038] Table 2: Tyrosinase inhibition rate

[0039] 2. Inhibit melanin test in the body: Materials: B16-F10 cells, culture medium (DMEM medium containing 10% fetal bovine serum), PBS, and microplate reader.

[0040] Experimental process: B16-F10 cells in the logarithmic growth phase were taken and the density was 1.5×10 6 Each well was inoculated into a 6-well plate, and 2 mL of culture medium was added to each well. The plate was cultured in a 37°C, 5% CO2 incubator for 24 hours, and the supernatant was discarded. The plate was rinsed twice with PBS and then divided into blank and sample groups. In the sample group, the above samples 1-11 were diluted to a concentration of 55 μg / mL using serum-free DMEM medium, and then added to the plate rinsed with PBS, 2 mL per well. In the blank group, 2 mL of serum-free DMEM medium was added to each well. Four parallels were set up for each group. The plate was then cultured in a 37°C, 5% CO2 incubator for 24 hours. The supernatant was discarded, and the plate was rinsed twice with PBS. The cells were cultured in a % CO2 incubator for 48 hours. The supernatant was aspirated and the remaining material (including cells) was collected in a centrifuge tube. The supernatant was discarded after centrifugation. Then, 200 μL of 1 mol / L NaOH solution was added and the cells were vortexed in an 80°C water bath for 1 hour. The absorbance of each vortex solution was measured at 405 nm using a microplate reader. The melanin inhibition rate was calculated according to the measured absorbance, where melanin inhibition rate = (blank group OD - 1 mol / L - sample group OD / blank group OD). The test results are shown in Table 3.

[0041] Table 3: Melanin inhibition rate

[0042] It can be seen from the test results in Table 2-3 that both the Uncaria sinensis leaf extract and the Emblica officinalis leaf extract can effectively inhibit the activity of tyrosinase and the formation of melanin, and both can greatly enhance the whitening and freckle-removing effect of cosmetics. In addition, it can be seen from the comparison of samples 1-11 that when the Uncaria sinensis leaf extract and the Emblica officinalis leaf extract are in the range of 1:0.2-8 by weight, the melanin and tyrosinase inhibition effects produced by the combination of the two are stronger than the effects of the Uncaria sinensis leaf extract and the Emblica officinalis leaf extract alone, indicating that the two can produce a synergistic effect in the above-mentioned ratio range. According to the comparison of samples 5-7 with other samples, it can be obtained that when the weight ratio is within the range of 1:0.8-1.5, the effect produced by the combination is better.

[0043] Example 2 The extracts of Uncaria sinensis leaves and Emblica officinalis leaves prepared above were used as raw materials and the amounts of the raw materials in weight percentages as shown in Table 4 were used to prepare essences.

[0044] Table 4: Essence composition

[0045] In Table 4, the preparation method of beetroot extract is as follows: beetroot is crushed, and a 30wt% ethanol solution is added according to a solid-liquid ratio of 1g:20mL, heated to 50°C and kept warm, and stirred at a speed of 100rpm for 3.5 hours, and then filtered through a 0.22μm filter membrane. The obtained filtrate is concentrated to 12.3% of the filtrate weight at 55°C and 0.0095MPa, and then freeze-dried at -45°C to a water content of 1.21wt% to obtain the beetroot extract.

[0046] The preparation method of holy basil leaf extract is as follows: take holy basil leaves and crush them, add a 32.5wt% ethanol solution according to the solid-liquid ratio of 1g:22mL, heat to 55°C and keep warm and stir at a speed of 100rpm for 4 hours, then filter with a 0.22μm filter membrane, and concentrate the obtained filtrate at 55°C and 0.0095MPa to 13.6% of the filtrate weight, and then freeze-dry at -45°C to a water weight of 1.13wt% to obtain the holy basil leaf extract.

[0047] The preparation method of the above-mentioned essence is as follows: add water to a reaction container and heat it to 60°C, keep it warm and start stirring at 120 rpm, add polyol (1,3-butylene glycol) and solubilizer (Tween-20, PEG-40 hydrogenated castor oil), stir for 0.3 hour, then add α-arbutin, glycyrrhizin, and asiaticoside and continue stirring for 0.3 hour, cool to 45°C, add Uncaria rhynchophylla leaf extract, Phyllanthus emblica leaf extract, beet root extract, holy basil leaf extract and excipients and continue stirring for 0.5 hour, let it stand at room temperature for aging for 2 hours, sterilize and package to obtain the essence.

[0048] The essence prepared above was tested to test the effect of the emulsion on promoting the repair and renewal (healing rate) of the keratinocyte cell line HaCaT cells.

[0049] Materials: HaCaT cells, DMEM medium containing 10% fetal bovine serum, DMEM medium, PBS.

[0050] Experimental process: HaCaT cells in the logarithmic growth phase were taken and the density was 4×10 6 Cells were seeded into 6-well plates at 100 μg / well and incubated at 37°C, 5% CO₂ until 80% confluency. A 200 μL pipette tip was used to evenly scratch the wound, followed by rinsing with PBS. The PBS and culture medium were then aspirated. The cells were then divided and dosed differently: For the serum group, the serum-free serum was diluted to 5 mg / mL in serum-free DMEM and added to 2 mL of the wells. For the blank group, serum-free DMEM was added to 2 mL of the wells. Four replicates were set up for each group. The cells were then incubated at 37°C, 5% CO₂ for 24 hours. The wound healing process was observed microscopically at the initial scratch and again after 24 hours. The healing rate was calculated based on the scratch area at each time point. The healing rate was calculated as (scratch area at 24 hours – scratch area at the initial time) / scratch area at the initial time. The test results are shown in Table 5.

[0051] Table 5: Healing rate test

[0052] According to the test results in Table 5, the serum prepared by the present application can effectively promote cell renewal and metabolism, is rich in moisturizing active ingredient polyol and active ingredients such as beetroot extract and basil leaf extract for promoting keratinocyte metabolism and renewal, and can effectively give the skin a tender and full state. According to the comparison of serums 1 and 3, it can be known that the use of beetroot extract and basil leaf extract in the serum of the present application can effectively promote the renewal and metabolic performance of keratinocytes. And according to the comparison of serums 1, 4-7, it can be known that when the beetroot extract and basil leaf extract are used together, the effect is better than when only one of them is used, and the combination of the two can synergistically improve the renewal rate and effect of keratinocytes, especially when the ratio of the two is in the range of 1:0.4-1, the effect is better.

[0053] The foregoing description describes only specific embodiments of the present application. Other modifications and variations can be made by those skilled in the art without departing from the spirit and scope of the present application. The appended claims are intended to cover all such modifications and variations as falling within the scope of the present application. Various modifications and variations can be made to the present application without departing from the spirit and scope of the present application; if these modifications and variations of the present application fall within the scope of the claims of the present application and their equivalents, the present application also intends to include the technical content of these modifications and variations.

Claims

1. A skin rejuvenation and freckle removal essence, characterized in that: The raw materials are: α-arbutin, glycyrrhizin, asiaticoside, Uncaria rhynchophylla leaf extract, Emblica officinalis leaf extract, Beetroot extract, Holy Basil leaf extract, polyol, solubilizer, excipients and water.

2. A skin rejuvenation and freckle removal essence according to claim 1, characterized in that: The auxiliary material is at least one of a preservative, a thickener, a pigment, a flavor, a pH regulator, and a chelating agent.

3. The skin rejuvenation and freckle removal essence according to claim 1, characterized in that: According to weight percentage, the raw materials are: 0.01-0.5% α-arbutin, 0.01-0.5% glabridin, 0.01-0.5% asiaticoside, 0.2-1.5% Uncaria rhynchophylla leaf extract, 0.2-1.5% emblica leaf extract, 0.2-1.5% beet root extract, 0.2-1.5% holy basil leaf extract, 5-20% polyol, 1-3% solubilizer, 0-2% excipients and the balance water.

4. The skin rejuvenation and freckle removal essence according to claim 1, characterized in that: The dosage of auxiliary materials is: 0-0.3% preservative, 0-0.8% thickener, 0-0.05% pigment, 0-0.05% fragrance, 0-0.5% pH regulator, 0-0.3% chelating agent.

5. The skin rejuvenation and freckle removal essence according to claim 1, characterized in that: The polyol includes at least one of sorbitol, ethylene glycol, polyethylene glycol, glycerol, propylene glycol, butylene glycol, hexylene glycol, pentylene glycol, and caprylyl glycol; And / or, the solubilizing agent includes at least one of Tween-20, Tween 60, and PEG-40 hydrogenated castor oil.

6. The skin rejuvenation and freckle removal essence according to claim 1, characterized in that: The weight ratio of the Uncaria sinensis leaf extract to the Emblica officinalis leaf extract is 1:0.8-1.

5.

7. The skin rejuvenation and freckle removal essence according to claim 1, characterized in that: The preparation method of the Uncaria sinensis leaf extract comprises: crushing the Uncaria sinensis leaves, adding 30-55 wt% ethanol solution, heating and extracting, filtering, concentrating and drying to obtain the Uncaria sinensis leaf extract; And / or, the preparation method of the emblica leaf extract comprises: crushing the emblica leaves, adding 25-50wt% ethanol solution for heating and extraction, filtering, concentrating and drying to obtain the emblica leaf extract.

8. The skin rejuvenation and freckle removal essence according to claim 1, characterized in that: The weight ratio of beetroot extract to holy basil leaf extract is 1:0.4-1; And / or, the beetroot extract and the holy basil leaf extract are both prepared by extracting with a 20-40 wt% ethanol solution.

9. A method for preparing a skin-rejuvenating and freckle-removing essence according to any one of claims 1 to 8, characterized in that: include: Add water to the reaction container and heat it, start stirring, add polyol and solubilizer, stir, then add α-arbutin, glabridin, and asiaticoside and continue stirring, cool, add Uncaria rhynchophylla leaf extract, Emblica officinalis leaf extract, Beetroot extract, Holy Ocimum sanctum leaf extract and auxiliary materials and continue stirring, let it stand for aging, sterilize and package to obtain the skin rejuvenation and freckle removal essence.

10. The preparation method according to claim 9, characterized in that Heat to 55-75°C.

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