Wild jujube cake and preparation method thereof

Abstract

The invention discloses a wild jujube cake and a preparation method thereof, relates to the technical field of homology of medicine and food, and adopts the technical scheme that the wild jujube cake is prepared from 3 parts of fried spina date seeds, 3 parts of wine steamed mulberries, 1 part of salt steamed black beans, 1 part of nine-steamed nine-sun-dried black sesames and 4 parts of refined honey. The traditional Chinese medicine can tonify the liver and the kidney, is used for treating insomnia, alopecia and leukotrichia caused by deficiency of liver blood, and particularly has a relatively obvious effect on senile insomnia and climacteric insomnia.

Description

Technical Field

[0001] The present invention relates to the technical field of medicine and food homology, and more particularly to a sour jujube cake and a preparation method thereof. Background Art

[0002] Sleep disorders are common in modern society. In 2020, approximately 700 million people worldwide suffered from insomnia. The disease has a protracted course and a high relapse rate after recovery. Long-term insomnia increases the risk of developing or developing comorbid physical and mental illnesses.

[0003] Insomnia has become a widespread problem in modern society. Its high prevalence and severe detrimental effects pose a significant challenge to the public's physical and mental health and have become a prominent issue affecting the public's quality of life. Refractory insomnia, in particular, presents a daunting challenge in clinical treatment. Patients often experience cognitive biases and emotional disturbances, manifesting as oversensitivity, suspicion, and frequent internal conflicts. Excessive worry about the consequences of insomnia further exacerbates their psychological burden. They often attempt to improve their condition by deliberately pursuing sleep. However, the conflicting feeling of "the more they want to sleep, the less they can" only worsens their insomnia symptoms and can even trigger negative emotions such as pessimism and depression, ultimately forming a vicious cycle that is difficult to break. Furthermore, long-term illness can trigger blood stasis, a pathological mechanism known as "long-term illness entering the meridians," further complicating the pathogenesis of insomnia. Overall, insomnia has diverse causes and complex pathogenesis. Traditional treatments, primarily based on Western medicine, often offer limited clinical efficacy due to issues such as daytime residual effects, addiction, rebound reactions, and other side effects. There is an urgent need to explore safer and more effective intervention strategies.

[0004] Currently, Western medicine is the main treatment for insomnia, but it has pain points such as receptor tolerance, rebound after withdrawal, and limited efficacy of subtypes. Although such drugs can quickly promote sleep, they cause side effects such as daytime sleepiness and liver damage. From the perspective of traditional Chinese medicine theory, insomnia belongs to the category of "insomnia" and "inability to sleep". The core of its pathogenesis lies in the miscommunication of yin and yang and the imbalance of viscera and qi, mainly liver blood deficiency type. The "Yellow Emperor's Internal Classic" proposes that "wei qi cannot enter yin and often stays in yang" is the root of insomnia. Although traditional Chinese medicine seeds have a calming effect, they are not targeted enough for insomnia of liver blood deficiency type. It is necessary to break through the single medication mode recorded in ancient books, and construct a three-dimensional compatibility system of "nourishing liver blood, replenishing kidney essence, clearing away heat and calming the mind" through four medicinal materials (fried Chinese medicine seeds - wine mulberry - salt black beans - processed sesame), and realize the synergistic effect of "1+1>2" in the optimization of the ratio of monarch, minister, assistant and envoy. In view of this, the inventors propose a Chinese medicine cake and a preparation method thereof. Summary of the Invention

[0005] The invention aims to provide a sour jujube cake and a preparation method thereof, which can nourish the liver and kidney and be used for insomnia, hair loss and gray hair caused by liver blood deficiency, and has a more obvious effect on senile insomnia and menopausal insomnia.

[0006] The technical problem of the present application is solved by the following technical solution:

[0007] The present application provides an acid jujube cake and a preparation method thereof, which is prepared from the following raw materials by weight:

[0008] 3 parts of fried acid jujube kernel, 3 parts of wine steamed mulberry, 1 part of salt steamed black bean, 1 part of nine steaming and nine drying black sesame, and 4 parts of refined honey.

[0009] The present application further provides a preparation method of the acid jujube cake, which comprises the following steps:

[0010] S1. The fried acid jujube kernel, wine steamed mulberry and salt steamed black bean are respectively ground into powder and sieved; the nine steaming and nine drying black sesame is crushed;

[0011] S2. The fried acid jujube kernel, wine steamed mulberry, salt steamed black bean and nine steaming and nine drying black sesame are mixed in a ratio of 3:3:1:1 and uniformly prepared for use;

[0012] S3. The refined honey is taken, and the refined honey is prepared into soft and hard pill blocks in a ratio of 2:1, which are then placed in a mold, flattened, and cut into pill blocks of appropriate size.

[0013] In combination with the second aspect, the present application is further provided as follows: in step S1, the fried acid jujube kernel is sieved through a No. 2 sieve, and the wine steamed mulberry and salt steamed black bean are sieved through a No. 3 sieve.

[0014] In summary, the present application has the following beneficial effects:

[0015] The fried acid jujube kernel is the monarch drug for calming the nerves and prolonging the deep sleep period. After the wine steamed mulberry and salt steamed black bean are steamed, their cool nature and the warm nature of the salt steamed black bean are balanced to maintain the balance of “moistening yin without stagnation and warming and tonifying without dryness”. The wine steamed mulberry and black bean are both the minister drugs of the prescription. The black sesame is prepared to eliminate the side effect of the raw black sesame of sliding the intestines, and it is used to counteract the astringency of the acid jujube kernel to ensure the stability of the intestinal flora, and it is the assistant drug of the prescription. The main effect is to tonify the liver and kidney, and it is used for insomnia, hair loss and white hair caused by liver blood deficiency, especially for senile insomnia and menopausal insomnia. The present prescription combines the four methods of “frying, steaming, roasting and exposure” and the combination wisdom of “monarch tonifying the spirit, minister moistening yin, assistant tonifying the kidney and minister regulating the collaterals”, which not only follows the traditional theory of “water and fire being in balance”, but also realizes the precise sleep regulation of multiple systems and multiple targets, and provides a model solution for the modernization of traditional Chinese medicine compounds. BRIEF DESCRIPTION OF DRAWINGS

[0016] Figure 1 is the body weight change of each group of rats in experimental example 1 of the present application;

[0017] Figure 2 is the content of DA in the serum of each group of rats in experimental example 1 of the present application;

[0018] Figure 3is the content of ACH in the serum of each group of rats in experimental example 1 of the present application;

[0019] Figure 4 is the content of 5-HT in the serum of each group of rats in experimental example 1 of the present application;

[0020] Figure 5 is the content of GABA in the serum of each group of rats in experimental example 1 of the present application (1 is a blank group, 2 is a model group, 3 is a positive group, 4 is a low-dose group of raw Semen Ziziphi Spinosae, 5 is a medium-dose group of raw Semen Ziziphi Spinosae, 6 is a high-dose group of raw Semen Ziziphi Spinosae, 7 is a low-dose group of fried Semen Ziziphi Spinosae, 8 is a medium-dose group of fried Semen Ziziphi Spinosae, 9 is a high-dose group of fried Semen Ziziphi Spinosae, 10 is a low-dose group of steamed Semen Ziziphi Spinosae, 11 is a medium-dose group of steamed Semen Ziziphi Spinosae, 12 is a high-dose group of steamed Semen Ziziphi Spinosae, and 13 is a excipient group);

[0021] Figure 6 is a hippocampal tissue section of each group of rats in experimental example 2 of the present application;

[0022] Figure 7 is the expression result of GABA in the hippocampal tissue of each group of rats in experimental example 2 of the present application (1 is a blank group, 2 is a model group, 3 is a positive group, 4 is a low-dose group of raw Semen Ziziphi Spinosae, 5 is a medium-dose group of raw Semen Ziziphi Spinosae, 6 is a high-dose group of raw Semen Ziziphi Spinosae, 7 is a low-dose group of fried Semen Ziziphi Spinosae, 8 is a medium-dose group of fried Semen Ziziphi Spinosae, 9 is a high-dose group of fried Semen Ziziphi Spinosae, 10 is a low-dose group of steamed Semen Ziziphi Spinosae, 11 is a medium-dose group of steamed Semen Ziziphi Spinosae, 12 is a high-dose group of steamed Semen Ziziphi Spinosae, and 13 is a excipient group);

[0023] Figure 8 is the expression result of 5-HT in the hippocampal tissue of each group of rats in experimental example 2 of the present application (1 is a blank group, 2 is a model group, 3 is a positive group, 4 is a low-dose group of raw Semen Ziziphi Spinosae, 5 is a medium-dose group of raw Semen Ziziphi Spinosae, 6 is a high-dose group of raw Semen Ziziphi Spinosae, 7 is a low-dose group of fried Semen Ziziphi Spinosae, 8 is a medium-dose group of fried Semen Ziziphi Spinosae, 9 is a high-dose group of fried Semen Ziziphi Spinosae, 10 is a low-dose group of steamed Semen Ziziphi Spinosae, 11 is a medium-dose group of steamed Semen Ziziphi Spinosae, 12 is a high-dose group of steamed Semen Ziziphi Spinosae, and 13 is a excipient group);

[0024] Figure 9 is the expression amount of CHRNA7 mRNA in the hypothalamus of each group of rats in experimental example 2 of the present application;

[0025] Figure 10 is the expression amount of DRD2 mRNA in the hypothalamus of each group of rats in experimental example 2 of the present application;

[0026] Figure 11 is the protein expression in the hypothalamus of each group of rats in experimental example 2 of the present application. DETAILED DESCRIPTION

[0027] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative work fall within the scope of the present application.

[0028] Embodiment 1

[0029] A jujube cake is prepared from the following raw materials by weight:

[0030] 3 parts of fried jujube kernels, 3 parts of wine-steamed mulberries, 1 part of salt-steamed black beans, 1 part of nine-steamed and nine-dried black sesame seeds, and 4 parts of refined honey.

[0031] Jujube kernel: sweet, sour, and flat. It is attributed to the liver, gallbladder, and heart meridians. It nourishes the heart and liver, calms the heart and tranquilizes the mind, astringes sweat, and generates saliva. It is used for restlessness, insomnia, palpitation, and dreaminess due to deficiency, excessive sweating, and thirst due to saliva deficiency.

[0032] Fried jujube kernel: take jujube kernel raw medicinal materials, remove impurities and broken shells, get clean kernels, and place them in a preheated to 160℃ frying machine, and uniformly stir-fry at a speed of 3 revolutions per minute, so that the seed coat cracking rate reaches 80%, and the aroma is strong without burning.

[0033] Mulberry: sweet, sour, and cold. It is attributed to the heart, liver, and kidney meridians. It nourishes yin and blood, generates saliva, and moistens dryness. It is used for liver and kidney yin deficiency, dizziness and tinnitus, palpitation and insomnia, premature graying, thirst due to saliva deficiency, internal heat and polydipsia, and constipation due to dryness.

[0034] Wine-steamed mulberry: mix mulberry and yellow rice wine at a ratio of 1:5, soak for 2 hours until the yellow rice wine is completely absorbed, steam at 100℃ under normal pressure for 120 minutes, and dry at 60℃ for standby;

[0035] Black bean: sweet and flat. It is attributed to the spleen and kidney meridians. It benefits the eyesight, nourishes blood, dispels wind, benefits water, and detoxifies. It is used for yin deficiency, thirst, dizziness and blurred vision, excessive sweating, kidney deficiency, edema, and numbness of the hands and feet.

[0036] Salt-steamed black bean: take clean black beans, soak them in water and salt at a ratio of 100:100:1 for 2 hours until the salt water is completely absorbed, steam for 120 minutes under normal pressure, and dry at 60℃ for standby;

[0037] Black sesame seed: sweet and flat. It is attributed to the liver, kidney, and large intestine meridians. It supplements the liver and kidney, benefits essence and blood, and moistens the intestines. It is used for deficiency of essence and blood, dizziness and blurred vision, tinnitus and deafness, premature graying, hair loss after illness, and constipation due to dryness.

[0038] Nine-steamed and nine-dried black sesame seed: take clean black sesame seeds, steam for 1 hour, dry for 12 hours, repeat the steaming for 9 times, and dry for standby.

[0039] Example 2: Preparation method of jujube cake

[0040] The above fried jujube seeds are ground to pass through a No. 2 sieve, the wine mulberries and salt black beans are ground to pass through a No. 3 sieve, respectively; and the nine steamed and nine dried black sesame seeds are broken. The fried jujube seeds, wine mulberries, salt black beans and nine steamed and nine dried black sesame seeds are mixed in a ratio of 3:3:1:1 to obtain a mixture. The mixture is mixed evenly and used as prepared. The refined honey is mixed with the mixture in a ratio of 2:1 to obtain a pill with a soft and hard degree, which is then placed in a mold, flattened, and cut into pills with a proper size.

[0041] Experimental Example 1: Study on the regulation of neurotransmitters in serum of insomnia rats with raw and processed jujube seeds

[0042] (1) Grouping and administration

[0043] The 130 healthy male SD rats were randomly divided into 13 groups, 10 rats in each group, namely a blank group (K), a model group (M), a positive group (Y), a raw jujube seed low-dose group (SD), a raw jujube seed medium-dose group (SZ), a raw jujube seed high-dose group (SG), a fried jujube seed low-dose group (CD), a fried jujube seed medium-dose group (CZ), a fried jujube seed high-dose group (CG), a steamed jujube seed low-dose group (ZD), a steamed jujube seed medium-dose group (ZZ), and a steamed jujube seed high-dose group (ZG). The rats were intraperitoneally injected at a fixed time every day. The model group (M), the positive group (Y), the raw jujube seed low-dose group (SD), the raw jujube seed medium-dose group (SZ), the raw jujube seed high-dose group (SG), the fried jujube seed low-dose group (CD), the fried jujube seed medium-dose group (CZ), the fried jujube seed high-dose group (CG), the steamed jujube seed low-dose group (ZD), the steamed jujube seed medium-dose group (ZZ), and the steamed jujube seed high-dose group (ZG) were intraperitoneally injected with 400 mg / (kg·d) of PCPA, and the blank group (K) was intraperitoneally injected with the same volume of normal saline, once a day for 3 consecutive days. After the modeling was successful, the low-dose group was orally administered with 1.5 g / (kg·d) of raw jujube seeds, fried jujube seeds and steamed jujube seeds, respectively, the medium-dose group was orally administered with 3 g / (kg·d) of raw jujube seeds, fried jujube seeds and steamed jujube seeds, respectively, the high-dose group was orally administered with 6 g / (kg·d) of raw jujube seeds, fried jujube seeds and steamed jujube seeds, respectively, the blank group (K) and the model group (M) were orally administered with the same volume of normal saline, and the positive group was orally administered with 0.52 mg / (kg·d) for 14 consecutive days, once a day. The hair, food intake and mental state of the rats in each group were observed, and the body weight of the rats was recorded on the 0th, 3rd, 6th, 9th, 12th, 15th, 17th day of the experiment, respectively.

[0044] (2) Sample collection

[0045] After the last administration for 24h, the rats were injected with 0.6mL / 100g of 20% urethane in the abdominal cavity, and blood was taken from the abdominal aorta. After standing for 30min, the blood was centrifuged at 3500r·min -1 for 10min, and the supernatant was used for the detection of rat 5-hydroxytryptamine (5-HT) ELISA kit.

[0046] Experimental results:

[0047] (1) Changes in body weight

[0048] The rats in the blank group had lustrous hair, normal circadian rhythm, and normal food and water intake. After modeling, the rats had dull hair, abnormal circadian rhythm, and decreased food and water intake. After administration, the above-mentioned conditions were alleviated in all groups except the model group. After modeling, the body weight of each group decreased except the blank group. After administration, the body weight of each group increased significantly, while the model group had a smaller increase. The largest increase was observed in the high-dose group of the fried Semen Ziziphi Spinosae. The results are shown in Figure 1 .

[0049] (2) Effects of raw and processed Semen Ziziphi Spinosae on GABA, ACH, DA, and 5-HT in serum of rats

[0050] After 14 days of administration, the DA content in the model group was higher than that in the blank group. Compared with the model group, the DA content in each administration group decreased. Except for the blank group, the lowest DA content was observed in the high-dose group of the fried Semen Ziziphi Spinosae, followed by the middle-dose group of the steamed Semen Ziziphi Spinosae. The results are shown in Figure 2 .

[0051] After 14 days of administration, the ACH content in the model group was higher than that in the blank group. Compared with the model group, the ACH content in each administration group decreased. The lowest ACH content was observed in the low-dose group of the steamed Semen Ziziphi Spinosae, followed by the high-dose group of the steamed Semen Ziziphi Spinosae. The results are shown in Figure 3 .

[0052] After 14 days of administration, the 5-HT content in the model group was lower than that in the blank group. Compared with the model group, the 5-HT content in each administration group increased. The highest 5-HT content was observed in the high-dose group of the fried Semen Ziziphi Spinosae, followed by the high-dose group of the steamed Semen Ziziphi Spinosae. The results are shown in Figure 4 .

[0053] After 14 days of administration, the GABA content in the model group was lower than that in the blank group. Compared with the model group, the GABA content in most administration groups increased. The highest GABA content was observed in the high-dose group of the steamed Semen Ziziphi Spinosae, the high-dose group of the fried Semen Ziziphi Spinosae, and the middle-dose group of the fried Semen Ziziphi Spinosae. The results are shown in Figure 5 .

[0054] Conclusion: In this study, the insomnia model of rats was replicated by intraperitoneal injection of PCPA, and the content of neurotransmitters in serum of rats in each group was determined by enzyme-linked immunoassay to clarify the specific effect of the drugs on neurotransmitters in insomnia rats and deduce the possible mechanism. The results showed that both raw and processed Zizyphi Spinosi Semen could improve the behavioral indicators of insomnia rats, increase the content of GABA and 5-HT in insomnia rats, and reduce the content of DA and ACH. The effects of different processed products of Zizyphi Spinosi Semen and different doses of the same processed product were different. In this experiment, the low-dose group of Zizyphi Spinosi Semen steamed product had the best effect on reducing ACH level, the high-dose group of Zizyphi Spinosi Semen steamed product, the high-dose and medium-dose groups of Zizyphi Spinosi Semen fried product had the best effect on increasing GABA level, and the high-dose group of Zizyphi Spinosi Semen fried product had the best effect on increasing 5-HT and reducing DA level. In general, the effect of different processed products of Zizyphi Spinosi Semen and different doses of the same processed product on neurotransmitters in serum of insomnia rats was as follows: fried product > steamed product > raw product, high dose > medium dose ≈ low dose.

[0055] Experimental Example 2: (II) Effect of raw and processed Zizyphi Spinosi Semen on dopamine / cholinergic pathway in insomnia rats

[0056] Experimental method:

[0057] (1) Grouping and drug administration

[0058] 130 healthy male SD rats were randomly divided into 13 groups, 10 rats in each group, respectively, blank group (K), model group (M), positive group (Y), low-dose group of raw Semen Ziziphi Spinosae (SD), medium-dose group of raw Semen Ziziphi Spinosae (SZ), high-dose group of raw Semen Ziziphi Spinosae (SG), low-dose group of fried Semen Ziziphi Spinosae (CD), medium-dose group of fried Semen Ziziphi Spinosae (CZ), high-dose group of fried Semen Ziziphi Spinosae (CG), low-dose group of steamed Semen Ziziphi Spinosae (ZD), medium-dose group of steamed Semen Ziziphi Spinosae (ZZ), high-dose group of steamed Semen Ziziphi Spinosae (ZG). The rats were intraperitoneally injected at a fixed time every day. The model group (M), positive group (Y), low-dose group of raw Semen Ziziphi Spinosae (SD), medium-dose group of raw Semen Ziziphi Spinosae (SZ), high-dose group of raw Semen Ziziphi Spinosae (SG), low-dose group of fried Semen Ziziphi Spinosae (CD), medium-dose group of fried Semen Ziziphi Spinosae (CZ), high-dose group of fried Semen Ziziphi Spinosae (CG), low-dose group of steamed Semen Ziziphi Spinosae (ZD), medium-dose group of steamed Semen Ziziphi Spinosae (ZZ), high-dose group of steamed Semen Ziziphi Spinosae (ZG) were intraperitoneally injected with 400 mg / (kg·d) of PCPA, and the blank group (K) was intraperitoneally injected with the same volume of normal saline, once a day for 3 consecutive days. After the modeling was successful, the low-dose group was given 1.5 g / (kg·d) of raw Semen Ziziphi Spinosae, fried Semen Ziziphi Spinosae and steamed Semen Ziziphi Spinosae by gavage at a fixed time every day, respectively; the medium-dose group was given 3 g / (kg·d) of raw Semen Ziziphi Spinosae, fried Semen Ziziphi Spinosae and steamed Semen Ziziphi Spinosae by gavage, respectively; the high-dose group was given 6 g / (kg·d) of raw Semen Ziziphi Spinosae, fried Semen Ziziphi Spinosae and steamed Semen Ziziphi Spinosae by gavage, respectively; the blank group (K) and the model group (M) were given the same volume of normal saline by gavage, and the positive group was given 0.52 mg / (kg·d) by gavage for 14 consecutive days, once a day. The hair, food intake and mental state of rats in each group were observed, and the body weight of rats was recorded on the 0th, 3rd, 6th, 9th, 12th, 15th and 17th day of the experiment.

[0059] (2) Sample collection

[0060] 24 hours after the last administration, the rats were intraperitoneally injected with 0.6 mL / 100 g of 20% urethane, and the abdominal aorta was taken blood after decapitation, and the hippocampus of the rats was quickly taken on ice and placed in 40% paraformaldehyde solution for fixation for immunohistochemistry and HE staining for observation and analysis; the hypothalamus was washed with normal saline and then divided into two EP tubes and frozen in liquid nitrogen, and stored at -80℃ for use. The hypothalamus was used to determine the mRNA expression of DRD2 and CHRNA7 and the protein expression of SLC6A3, ChRM2, CHRNA7 and DRD2.

[0061] (3) RT-PCR method for determining the mRNA expression of DRD2 and CHRNA7 in the hypothalamus of rats

[0062] According to the specified steps, the total RNA was extracted, its concentration and purity were determined, and after meeting the requirements, the cDNA was synthesized according to the reverse transcription kit instructions. The reaction system was configured according to the kit instructions, and the reaction was carried out on the PCR instrument by selecting the reaction conditions. The primer sequence is shown in Table 20, 2- △△ The relative expression amount of the target gene in each sample was calculated by CT method.

[0063] Table 20 primer sequence table

[0064]

[0065] (4) Western Blot method for determining the protein expression of SLC6A3, ChRM2, CHRNA7 and D2 in the hypothalamus of rats

[0066] The hypothalamus of each group of rats was ground with a tissue homogenizer, and the protein was extracted by low-temperature lysis with RIPA lysis buffer. The protein concentration was determined by Bradford method, and the known concentration of protein was denatured by setting the program of the gene amplifier. Then electrophoresis, electrotransfer, 5% skimmed milk room temperature shaking bed blocking, adding diluted primary antibody and internal reference 4℃ shaking bed incubation overnight, then adding diluted secondary antibody room temperature shaking bed incubation for 1h, adding appropriate amount of ECL luminescent liquid exposure imaging, analyzing the gray value of the band.

[0067] Experimental results

[0068] 1. Light microscope observation results of hippocampal tissue sections of rats in each group

[0069] The normal group: the rat hypothalamic cortex nerve cells are rich in shape, uniform distribution, the hypothalamic nucleus is arranged densely, and the structure is complete. A large number of nerve cells in the cortex of the model group are vacuolated, the neurons in the thalamic nucleus are atrophic, the arrangement is loose, the cell outline is not clear, and the cell number is reduced. The low, medium and high groups of Zizyphus jujuba var. spinosa, fried products, steamed products, positive group and auxiliary material group of rats can restore most of the nerve cells in the hypothalamic cortex and thalamic nucleus, and the arrangement is relatively neat. Occasionally, small focal cell atrophy and halo around the cells can be seen. The results are shown in Figure 6 .

[0070] The normal group has more GABA cells in the hypothalamus and uniform distribution, and the cytoplasm is colored darker. The number of GABA cells in the hypothalamus of the model group is reduced and unevenly distributed, and the cytoplasm is colored lighter. The number of GABA cells in the low, medium and high groups of Zizyphus jujuba var. spinosa, fried products, steamed products, positive group and auxiliary material group is increased to varying degrees, and the color is darker. The results are shown in Figure 7 .

[0071] The normal group has more 5-HT cells in hypothalamus and the cells are uniformly distributed and have deep color. The model group has less 5-HT cells in hypothalamus and the cells are unevenly distributed and have light color. The low, medium and high groups of the raw, fried and steamed products of Semen Ziziphi Spinosae, the positive group and the auxiliary material group have 5-HT cells in different degrees, and the color is deep. The results are shown in Figure 8 .

[0072] After 14 days of administration, compared with the blank group, the expression of DRD2 mRNA in the model group is high; compared with the model group, the administration groups can reduce the expression of DRD2 mRNA in the hypothalamus of insomnia rats, and the lowest expression is the low-dose group of steamed Semen Ziziphi Spinosae. The results are shown in Figure 10 .

[0073] After 14 days of administration, compared with the blank group, the expression of SLC6A3, ChRM2, CHRNA7 and DRD2 protein in the model group is high; compared with the model group, the administration groups can reduce the expression of SLC6A3, ChRM2, CHRNA7 and DRD2 protein in the hypothalamus of insomnia rats. The results are shown in Figure 11 .

[0074] Experimental Example 2 Conclusion: In this study, the insomnia rat model was replicated by intraperitoneal injection of PCPA, and the hypothalamus of rats in each group was taken for pathological section, immunohistochemistry, and determination of the expression of CHRNA7, DADR2 mRNA and the expression of SLC6A3, ChRM2, CHRNA7 and DRD2 protein. The results show that the water decoction of raw and processed Semen Ziziphi Spinosae can improve the pathological state of the hypothalamic section of rats caused by insomnia and the abnormal state of GABA and 5-HT positive cells in the hypothalamus; it can also reverse the increase of CHRNA7, DADR2 mRNA expression and SLC6A3, ChRM2, CHRNA7 and DRD2 protein expression in the hypothalamus of rats caused by insomnia through the DA / ACH pathway, and play a role in sedation and hypnotism.

[0075] Finally, it should be noted that the above description is only the preferred embodiments of the present application and is not intended to limit the present application. Although the present application has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent replacements to some technical features. Any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application shall be included in the protection scope of the present application.

Claims

1. A sour jujube cake, characterized by: It is prepared from the following raw materials in parts by weight: 3 parts of stir-fried sour jujube kernels, 3 parts of steamed mulberries with wine, 1 part of steamed black beans with salt, 1 part of steamed and dried black sesame seeds, and 4 parts of refined honey.

2. A method for preparing a sour jujube cake, characterized in that: The steps include: S1. Grind the stir-fried sour jujube kernels, wine-cooked mulberries, and salted black beans into powder and sieve separately; crush the steamed and dried black sesame seeds nine times; S2. Stir-fry sour jujube kernels, wine mulberries, salt black beans, and steamed and sun-dried black sesame seeds in a ratio of 3:3:1:1 and mix thoroughly. S3. Take refined honey and make pills of appropriate hardness and softness with a ratio of medicinal powder to refined honey of 2:

1. Place them in a mold and flatten them. Then cut them into pills of appropriate size.

3. The method for preparing a sour jujube cake according to claim 2, wherein: In step S1, the fried sour jujube kernels are passed through a No. 2 sieve, and the wine-cured mulberries and salted black beans are passed through a No. 3 sieve.

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