Preparation method and application of plant compound essential oil

Abstract

The invention relates to a skin preparation, in particular to a preparation method and application of plant compound essential oil. The plant compound essential oil is prepared from calendula officinalis infused oil, rose hip oil, sesame oil, fennelflower oil, lemon verbena oil, pelargonium graveolens oil, Damascus rose oil, clary sage oil, wormwood oil, chaste tree oil, European red pine oil and other raw materials, and the obtained compound essential oil is excellent in performance and has a good use effect.

Description

TECHNICAL FIELD

[0001] The present application relates to skin preparations, in particular to a preparation method of a plant compound essential oil and application thereof. BACKGROUND

[0002] The compound essential oil is prepared by scientific proportioning of single essential oil and base oil, and has multi-dimensional physiological regulation effect in the fields of massage, breast enhancement and female health, and the effect is based on transdermal absorption and targeting mechanism of active ingredients.

[0003] The essential oil is massaged to the skin, penetrates into the inner layer through the skin, enhances the local blood flow speed to accelerate the penetration of terpene small molecules in the essential oil through the stratum corneum, the base oil acts as a carrier to enhance the diffusion efficiency of fat-soluble ingredients, part of the essential oil contains phytoestrogens (such as anethole in fennel), which can temporarily activate the estrogen receptors of mammary gland cells, promote the hyperplasia of acini, stimulate the PPARγ pathway of adipocytes, and increase the volume of breast fat pad; part of the essential oil contains limonene, gingerene and trans- caryophyllene, which can relieve excessive contraction of uterine smooth muscle through abdominal massage; in addition, part of the essential oil contains terpinen-4-ol, terpenes and phenoxyethanol, which can destroy the cell membrane of Candida albicans.

[0004] The compound essential oil on the market currently has single effect or multiple functions but uneven efficacy strength. Therefore, it is a technical problem to be solved to provide a compound essential oil with multiple functions and uniform efficacy strength. SUMMARY

[0005] In view of the above technical problems, the present application provides a preparation method of a plant compound essential oil and application thereof.

[0006] The present application first provides a plant compound essential oil, and the raw material composition comprises chrysanthemum diplopappus infusion oil, rose hip oil, sesame oil, nuxvomica oil, lemon verbena oil, geranium oil, damask rose oil, clary sage oil and artemisia oil.

[0007] The clary sage oil, geranium oil, damask rose oil and acacia oil have estrogen-like effect, can stimulate hormones, and make the chest plump and firm; the clary sage oil contains linalyl acetate and other components, which can stimulate the PPARγ pathway of adipocytes; the geranium oil is also believed to stimulate the growth of mammary tissue. The lemon verbena oil and artemisia oil can be used for mammary gland dredging, the lemon verbena oil can improve deep sclerosis and soothe pain, and the artemisia oil is combined with body acupuncture to improve the shape of the breast.

[0008] In addition, the calendula infusion oil contains active ingredients such as flavonoids, which can help repair the skin, relieve minor burns, abrasions, or skin inflammation after repair; it has a certain moisturizing and soothing effect on sensitive or dry skin, and can reduce skin itching, tightness and discomfort. Rosehip oil is rich in vitamin A (retinol precursor) and essential fatty acids (such as linoleic acid and alpha-linolenic acid), which can help promote skin cell turnover, reduce fine lines, fade scars or stretch marks, and make the skin smoother; its oily texture can form a barrier on the skin surface to reduce water loss. As a carrier oil, sesame oil is often used for massage, which can increase the lubricity of the skin and relieve dry skin; it contains a small amount of antioxidant ingredients (such as sesamin), which may have a weak UV protection effect. The composition of the black seed oil, such as vitamin K, can regulate the immune system; it can also help relieve cough, nasal congestion and other symptoms. Lemon verbena oil has a fresh aroma and is often used in aromatherapy to relieve stress, anxiety, improve mood, and have a certain calming effect, and may also help relieve indigestion and abdominal distension. The oil of the rosmarinus officinalis has a certain regulating effect on female hormones and can relieve premenstrual discomfort (such as mood swings and breast pain); it can also be used as one of the ingredients of natural insect repellents. Damask rose oil has a significant soothing effect and can improve depression and anxiety, improve mood, and help promote blood circulation in the skin, making the skin appear rosy. Clary sage oil can be used to relieve menopausal symptoms (such as hot flashes and night sweats) or irregular menstruation, and it has an effect on estrogen levels; it can also be used as an aromatherapy ingredient to help relax the body and mind, improve sleep quality, and relieve tension. Mugwort oil has the effect of warming the meridians and dispelling cold, which can help relieve pain caused by cold and dampness (such as joint pain and menstrual pain) when used externally or moxibustion; its components such as eucalyptol and camphor can inhibit bacteria and fungi.

[0009] In one specific embodiment, the plant compound essential oil comprises, by weight: 45-55 parts of calendula infusion oil, 25-35 parts of rosehip oil, 5-10 parts of sesame oil, 2-7 parts of black seed oil, 0.5-1.5 parts of lemon verbena oil, 0.5-1.5 parts of rosmarinus officinalis oil, 0.2-0.7 parts of damask rose oil, 0.2-0.7 parts of clary sage oil, and 0.2-0.5 parts of mugwort oil.

[0010] In one specific embodiment, the plant compound essential oil further comprises acacia oil and European red pine oil. The weight of acacia oil can be 0.5-5 parts. The weight of European red pine oil can be 0.5-5 parts.

[0011] In one specific embodiment, the preparation method of acacia oil comprises: drying acacia fruit, crushing, adding ethanol and water (ethanol and water weight ratio 2-5:1), heating and stirring to extract, then filtering and concentrating to obtain.

[0012] In one embodiment, the preparation method of the European pine oil comprises: drying, crushing, adding ethanol and water (weight ratio of ethanol and water 2-5:1), heating and stirring extraction, then filtering and concentrating to obtain.

[0013] The Vitex oil indirectly balances the progesterone and estrogen levels by inhibiting the prolactin (PRL) secretion of the anterior pituitary, and reduces chronic inflammation caused by hormone imbalance (such as endometriosis, breast hyperplasia); the dopamine receptor agonist contained therein can reduce the luteal function caused by hyperprolactinemia, and relieve premenstrual breast pain and uterine inflammation. It is rich in monoterpenes (such as sabinene and limonene), which can inhibit the synthesis of COX-2 enzyme and prostaglandin E2 (PGE2), reduce tissue redness, heat and pain, and improve skin inflammation such as dermatitis and urticaria. In addition, the Vitex oil also has excellent antioxidant and strong free radical scavenging ability, and good skin moisturizing performance. The European pine oil contains α / β-pinene, which can stimulate the adrenal glands to secrete natural cortisol, inhibit the release of TNF-α, IL-6 and other pro-inflammatory factors, and significantly relieve allergic diseases (such as hay fever and allergic rhinitis) and arthritis; it also contains active ingredients such as catechin, epicatechin and ferulic acid, which can scavenge free radicals, inhibit matrix metalloproteinase activity, protect collagen from ultraviolet degradation, and have excellent antioxidant effect.

[0014] In one embodiment, the weight ratio of the Vitex oil and the European pine oil in the plant compound essential oil is 1:0.5-2.

[0015] In one embodiment, the plant compound essential oil further comprises vitamin E, and the weight part of vitamin E is 2-6 parts.

[0016] The application also provides a composition of the Vitex oil and the European pine oil. The combination of the two can enhance the antioxidant and anti-inflammatory effects. When the weight ratio of the Vitex oil and the European pine oil is 1:0.5-2, the effect is better.

[0017] The application also provides a preparation method of the plant compound essential oil, which comprises: preparing raw materials, adding the raw materials into a reaction kettle, stirring and mixing under stirring, standing and aging, filling and weighing, and obtaining the plant compound essential oil. The stirring and mixing time is 0.5-1.5 hours, and the standing and aging time is 0.5-1.5 hours.

[0018] The application also provides the application of the plant compound essential oil in preparing a composition. The composition comprises: 1-10% plant compound essential oil, 10-20 polyhydric alcohol, 3-7% emulsifier and the rest water. The emulsifier comprises sodium di(laureth-7) citrate and PPG-8-ceteth-20 at a weight ratio of 1:1-2. The polyhydric alcohol comprises at least one of propylene glycol, ethylene glycol, butylene glycol, glycerol and sorbitol.

[0019] The application also provides application of the plant compound essential oil in preparation of a massage preparation, wherein the plant compound essential oil is used in an amount of 0.1-15% in the massage preparation. The massage preparation can improve the breast enhancement effect, has a calming effect, can regulate and improve female endocrine and menstrual problems, and can also moisturize and soothe the skin.

[0020] Compared with the prior art, the application has the following effects.

[0021] The present application adopts raw materials including calendula infusion oil, rosehip oil, sesame oil, nigger grass oil, lemon verbena oil, geranium oil, damask rose oil, clary sage oil, wormwood oil, aspen tree oil and European red pine oil to prepare a plant compound essential oil, and the obtained compound essential oil has excellent performance and good use effect. The clary sage oil, geranium oil, damask rose oil and aspen tree oil have estrogen-like effect, can stimulate hormones and make the chest plump and firm. The clary sage oil contains linalyl acetate and other components, can stimulate the PPARγ pathway of adipocytes. The geranium oil is also considered to stimulate breast tissue growth. The lemon verbena oil and wormwood oil can be used for breast dredging, the lemon verbena oil can improve deep sclerosis and soothe pain. The wormwood oil is combined with body acupuncture to improve breast shape. The calendula infusion oil contains flavonoid active ingredients, has an auxiliary effect on skin repair, helps to relieve the repair after slight burns, abrasions or skin inflammation, has a certain moisturizing and soothing effect on sensitive or dry skin, reduces skin itching, tightness and discomfort. The rosehip oil is rich in vitamin A (retinol precursor) and essential fatty acids (such as linoleic acid and alpha-linolenic acid), can help promote skin cell renewal, reduce fine lines, fade scars or stretch marks, and make the skin smoother. The oil texture of the rosehip oil can form a barrier on the skin surface to reduce water loss. The sesame oil is used as a carrier oil, is often used for massage, can increase the lubricity of the skin, is used for body care and relieves dry skin. The sesame oil contains a small amount of antioxidant components (such as sesamin), and has a weak ultraviolet protection effect. The nigger grass oil has a certain regulating effect on the immune system due to the components such as vitamin K; and can help relieve symptoms such as cough and nasal congestion. The lemon verbena oil has a fresh aroma, is often used in aromatherapy to relieve stress and anxiety, helps to improve mood, has a certain nerve calming effect, and can also help relieve indigestion and abdominal distension. The geranium oil has a certain regulating effect on female hormones, can relieve premenstrual discomfort (such as mood swings and breast pain), and can also be used as one of the components of natural insect repellents. The damask rose oil has significant soothing effect, can improve depression and anxiety, improve mood, and help promote skin blood circulation to make the skin appear rosy. The clary sage oil can be used to relieve menopausal symptoms (such as hot flashes and night sweats) or irregular menstruation, has an effect on estrogen levels, and can also be used as an aromatherapy component to help relax the body and mind, improve sleep quality and relieve tension. The wormwood oil has the effect of warming the meridians and dispelling cold, and can help relieve pain caused by cold and dampness (such as joint pain and menstrual pain) when used externally or moxibustion. The components such as eucalyptol and camphor in the wormwood oil can inhibit bacteria and fungi.

[0022] The Eucommia ulmoides oil and the Pinus koraiensis oil used in the application have good free radical scavenging and anti-inflammatory effects; in the range of 1:0.1 to 1:7 in weight ratio, the two have a synergistic relationship in terms of antioxidant and anti-inflammatory effects; when the weight ratio changes from 1:0.1 to 1:7, the composition of the two has a trend of first increasing and then decreasing in terms of enhancing antioxidant and anti-inflammatory effects; when the ratio reaches 1:0.5-2, the matching effect is better, and the anti-inflammatory and antioxidant performance is better.

[0023] The product containing the compound essential oil prepared by the application has excellent stability, and the ZETA values of the normal temperature and temperature resistance stability tests are-59.03 mV and-50.76 mV, respectively, and the product has good stability. In the application, sodium di(laureth-7) citrate and PPG-8-ceteth-20 are used to effectively stabilize the compound essential oil, so that the stability of the product is significantly improved, and the synergistic effect of the two is better than that of a single use, and when the ratio of the two is 1:1-2, the stability of the product is further improved, and the storage and application effect of the product are effectively improved. BRIEF DESCRIPTION OF DRAWINGS

[0024] Figure 1 : Free radical scavenging test results of each group.

[0025] Figure 2 : Anti-inflammatory test results of each group.

[0026] Figure 3 : A1 value of stability test of each group. DETAILED DESCRIPTION

[0027] The application will be described in detail below in conjunction with specific embodiments, but the application is not limited to these specific embodiments. In addition, unless otherwise specified, the raw materials used in the specific embodiments of the application are obtained by commercial purchase.

[0028] Calendula soaking oil: Bai Caofang set calendula flower soaking oil, Jiangxi Bai Caofang Biological Technology Co., Ltd. Rose fruit oil: item number R909035, Macklin Reagent. Sesame oil: item number S905724, Macklin Reagent. Nigella damascena oil: Jiangxi Xinsen Natural Plant Oil Co., Ltd. Lemon verbena oil: Ji'an Huatian Precious Chinese Herbal Medicine Biological Product Factory. Myrtle geranium oil: item number G905687, Macklin Reagent. Damask rose oil: Jiangxi Cedrus Natural Medicinal Oil Co., Ltd. Happy chamomile oil: Jiangxi Global Natural Spices Co., Ltd. Mugwort oil: item number W905688, Macklin Reagent.

[0029] Preparation method of Zanthoxylum bungeanum oil: Zanthoxylum bungeanum fruits are dried, crushed, and particles with a particle size of less than 0.3 mm are selected. Ethanol and water are added, wherein the weight ratio of ethanol to water is 4:1, and the ratio of the particles to the total weight of ethanol and water is 1:23. The mixture is heated to 45°C, and stirred at 100 rpm for 6 hours. Then, the mixture is filtered, and concentrated at 60°C and 0.009 MPa to obtain Zanthoxylum bungeanum oil with a water content of 1.64% by weight.

[0030] Preparation method of Pinus sylvestris oil: Pinus sylvestris fruits are dried, crushed, and particles with a particle size of less than 0.3 mm are selected. Ethanol and water are added, wherein the weight ratio of ethanol to water is 4.5:1, and the ratio of the particles to the total weight of ethanol and water is 1:20. The mixture is heated to 50°C, and stirred at 100 rpm for 5.5 hours. Then, the mixture is filtered, and concentrated at 60°C and 0.009 MPa to obtain Pinus sylvestris oil with a water content of 1.73% by weight.

[0031] I. Free radical scavenging and anti-inflammatory tests 1. Free radical scavenging test Prepare the test samples according to the contents in Table 1 below, and measure the weight.

[0032] Table 1: Sample composition Working solution: prepare a DPPH solution with 0.12 mg / mL of DPPH and 95% ethanol.

[0033] Test group: dilute the above samples to a concentration of 0.045 mg / mL with 95% (V / V) ethanol to obtain a mixture, and then mix the mixture with the working solution according to the ratio of mixture: working solution = 0.5 mL: 2.5 mL to obtain the test solution of the test group.

[0034] Blank group: mix according to the ratio of 95% (V / V) ethanol: working solution = 0.5 mL: 2.5 mL to obtain the test solution of the blank group.

[0035] Store the test solutions of the above blank group and test group at room temperature and away from light for 30 minutes. Then, measure the absorbance OD value at a wavelength of 517 nm, and set three parallel groups. Calculate the DPPH free radical scavenging rate = 100% x (1-OD test group / OD blank group), wherein OD test group is the OD value of the test solution of the test group, and OD blank group is the OD value of the test solution of the blank group. The results are shown in Table 2 and Figure 1 .

[0036] Table 2: Free radical scavenging test 2. Anti-inflammatory test Test subjects and consumables: RAW264.7 cells; ELISA kit; culture medium, Gibco BenchStable DMEM medium.

[0037] Test groups: blank group, model group and experimental group (samples 1-9 above).

[0038] Take RAW264.7 cells in the logarithmic growth phase and plate them at 1.0×10 5 Each well was inoculated into a 24-well plate, 0.5 mL of culture medium was added to each well, and cultured in a constant temperature incubator at 37°C and 5% CO2 for 24 hours. 1 μg / mL of LPS was added to the wells of each group except the blank group to establish a cell inflammation model, and 0.5 mL of test solution was also added to the wells of the experimental group (samples 1-9 were used in turn with the working solution to prepare a 0.02 wt% test solution, and the working solution consisted of 0.2 wt% Tween + 0.5% glycerol and the remaining culture medium). 0.5 mL of culture medium was also added to the wells of the model group; only 0.5 mL of culture medium was added to the blank group; the above setting was repeated in 3 parallel groups. Then each group of well plates was incubated in a constant temperature incubator at 37°C and 5% CO2 for 30 hours. The supernatant was collected, and the concentrations of TNF-α and IL-6 were detected according to the instructions of the ELISA kit. The results are shown in Tables 3 and Figure 2 .

[0039] Table 3: Anti-inflammatory test According to Table 2-3 and Figures 1-2 The results show that both Chaste tree oil and Scots pine oil obtained by the present invention have good free radical scavenging and anti-inflammatory effects. Within a weight ratio range of 1:0.1 to 1:7, the two exhibit a synergistic relationship in terms of antioxidant and anti-inflammatory effects. When the weight ratio varies from 1:0.1 to 1:7, the combined composition shows a trend of first increasing and then decreasing in enhancing antioxidant and anti-inflammatory effects. In particular, when the ratio reaches 1:0.5-2, the combination has a better effect and better anti-inflammatory and antioxidant properties.

[0040] 2. Application and Testing Preparation of compound essential oils: Compound essential oil 1: Weigh the following raw materials by weight: 48.4 parts of calendula infused oil, 30 parts of rosehip oil, 7.8 parts of sesame oil, 3.8 parts of black cumin oil, 0.8 parts of lemon verbena oil, 0.8 parts of geranium oil, 0.5 parts of damask rose oil, 0.5 parts of clary sage oil, 0.5 parts of wormwood oil, 2.2 parts of chaste tree oil, and 2.2 parts of Scots pine oil. Add the raw materials to a reactor and stir at 100 rpm for 1 hour. Then, let stand at room temperature for 1 hour, fill and weigh to obtain a compound plant essential oil.

[0041] Compound essential oil 2: raw materials are weighed according to parts by weight: 55 parts of calendula officinalis infusion oil, 25 parts of rose hip oil, 5 parts of sesame oil, 6 parts of black nightshade oil, 1.5 parts of lemon verbena oil, 0.5 parts of lemongrass oil, 0.2 parts of damask rose oil, 0.2 parts of happy sage oil, 0.2 parts of wormwood oil, 2.5 parts of aspidosperma quebracho-blanco oil and 1.9 parts of European red pine oil. The raw materials are added to the reaction kettle, and stirring is carried out at a stirring speed of 100 rpm for 1.2 hours, then the mixture is aged at room temperature for 0.8 hours, and then filled and weighed to obtain the plant compound essential oil.

[0042] The above compound essential oil is used to prepare products, and the product composition is shown in Table 4, and the amount is measured in percentage by weight; wherein λ is the weight ratio of sodium di (laureth-7) citrate and PPG-8-cetyl alcohol polyether-20, product 2 uses compound essential oil 2, and other products use compound essential oil 1.

[0043] Table 4: Product composition The preparation method of the above composition is as follows: water is added to a container, polyhydric alcohol (1,2-propanediol, ethylene glycol, glycerol) and emulsifier (sodium di (laureth-7) citrate and PPG-8-cetyl alcohol polyether-20) are added, and stirring is carried out at 100 rpm for 0.2 hours, then the compound essential oil is added, and the stirring speed is increased to 200 rpm for 1 hour to obtain the composition.

[0044] The above composition is tested for ZETA potential.

[0045] Normal temperature test: the above composition is mixed with water at a weight ratio of 1:40, stirred at a speed of 300 rpm for 1 hour, and then placed at room temperature for 2 hours to obtain a test solution, and the normal temperature ZETA potential of the test solution is tested and recorded as A1. Heat resistance test: after testing the normal temperature ZETA potential, the test solution is stored in an environment of 40℃ for 5 days, and the heat resistance ZETA potential is tested and recorded as A2. The specific test results are shown in Table 5.

[0046] Table 5: ZETA potential According to the test results in Table 5, the product containing the compound essential oil prepared by the present application has excellent stability, and the ZETA potential of the normal temperature and heat resistance stability tests reaches-59.03 mV and-50.76 mV, which has good stability. According to the test results of products 1, 3-6, sodium di (laureth-7) citrate and PPG-8-cetyl alcohol polyether-20 can effectively stabilize the compound essential oil, so that the stability of the product is significantly improved, the synergistic effect of the two is better than the single use effect, and when the proportion is 1:1-2, the stability effect of the product is better, and the storage, application and use effect of the product are improved.

Claims

1. A plant compound essential oil, characterized in that: The raw materials are: calendula infused oil, rosehip oil, sesame oil, black cumin oil, lemon verbena oil, geranium oil, Damascus rose oil, clary sage oil and wormwood oil.

2. The plant compound essential oil according to claim 1, wherein The raw material composition is as follows, in parts by weight: 45-55 parts of calendula infused oil, 25-35 parts of rosehip oil, 5-10 parts of sesame oil, 2-7 parts of black cumin oil, 0.5-1.5 parts of lemon verbena oil, 0.5-1.5 parts of geranium oil, 0.2-0.7 parts of Damascus rose oil, 0.2-0.7 parts of clary sage oil and 0.2-0.5 parts of wormwood oil.

3. The plant compound essential oil according to claim 1, wherein Also includes chaste tree oil and Scots pine oil.

4. The plant compound essential oil according to claim 3, wherein The weight ratio of chaste tree oil to Scots pine oil is 1:0.5-2.

5. The plant compound essential oil according to claim 1, characterized in that Also includes vitamin E.

6. A method for preparing a plant compound essential oil according to any one of claims 1 to 5, characterized in that: include: Prepare raw materials, add the raw materials into the reactor, start stirring to stir and mix, then let it stand for aging, fill and weigh, and obtain the plant compound essential oil.

7. The preparation method according to claim 6, characterized in that The stirring and mixing time is 0.5-1.5 hours, and the standing and aging time is 0.5-1.5 hours.

8. Use of the plant compound essential oil according to any one of claims 1 to 5 in preparing a composition, characterized in that: The composition comprises: 1-10% of plant compound essential oil, 10-20% of polyol, 3-7% of emulsifier and the balance of water.

9. The use according to claim 8, characterized in that The emulsifier includes sodium di(laureth-7) citrate and PPG-8-ceteth-20 in a weight ratio of 1:1-2.

10. Use of the plant compound essential oil according to any one of claims 1 to 5 in preparing a massage preparation, characterized in that: The amount of the plant compound essential oil in the massage preparation is 0.1-15%.

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