Antioxidant composition containing polygala tenuifolia extract as well as preparation method and application of antioxidant composition

Abstract

The invention provides an antioxidant composition containing a polygala tenuifolia extract as well as a preparation method and application of the antioxidant composition. The antioxidant composition comprises the polygala tenuifolia extract and isoprene glycol, flavonoids contained in the polygala tenuifolia extract can inhibit oxidation reaction and can effectively resist aging; the polygala tenuifolia extract has the advantages that skin cuticle interlaminar lipid can be temporarily opened by the aid of the isoprene glycol, accordingly, more water-soluble components which cannot easily enter the polygala tenuifolia extract can enter the skin, permeation effects of the polygala tenuifolia extract on cuticles can be improved, and the oxidation resistance of the polygala tenuifolia extract can be effectively improved. According to the preparation method of the polygala tenuifolia extract, waste of natural resources is avoided, the utilization rate of stems and leaves of polygala tenuifolia can be increased, and greater economic benefits can be obtained.

Description

Technical Field

[0001] The present application belongs to the technical field of natural extract skin care products, and in particular relates to an antioxidant composition containing Polygala tenuifolia extract, and a preparation method and application thereof. Background Art

[0002] Polygala tenuifolia is a commonly used ingredient in traditional Chinese medicine. It is a perennial herb that is mainly produced in Northeast, North, Northwest, and Central my country. Polygala tenuifolia is classified as a "major commonly used wild medicinal species with severely reduced resources." The root of Polygala tenuifolia is the main medicinal component, while the stems and leaves (known as "small grass" in ancient times) are mostly considered non-medicinal parts and are usually discarded directly after harvesting. The stems and leaves of Polygala tenuifolia contain antioxidant ingredients such as total flavonoids and mangiferin, which have economic value. Discarding them directly is a serious waste of natural resources. Some companies have tried to crush the stems and leaves and use them as feed additives or plant fertilizers, but the economic benefits are still very low.

[0003] In order to improve the utilization rate of Polygala tenuifolia stems and leaves and obtain greater economic benefits, the Polygala tenuifolia stems and leaves can be prepared into a Polygala tenuifolia extract for use in preparing antioxidant cosmetics. Therefore, developing an antioxidant composition containing the Polygala tenuifolia extract is an urgent problem to be solved. Summary of the Invention

[0004] The present invention provides an antioxidant composition containing a polygala root extract and a preparation method and application thereof to solve the problems existing in the related art. The technical solution is as follows:

[0005] In a first aspect, the present invention provides an antioxidant composition containing a Polygala tenuifolia extract, comprising the following components in parts by weight:

[0006] Polygala tenuifolia extract: 0.5-2 parts; isopentyl glycol: 0.5-2 parts; adjuvant: 4.41-24.1 parts; solvent: 74.9-91.59 parts.

[0007] In one embodiment, the auxiliary agent is one or a combination of two or more of a moisturizer, a stabilizer, a thickener, a preservative or a soothing agent.

[0008] In one embodiment, the moisturizer includes one or a combination of two or more of propylene glycol, butylene glycol or glycerin; the stabilizer is EDTA-disodium; the thickener is xanthan gum and / or sodium polyacrylate; the preservative is one or a combination of two or more of chlorphenesin, methyl ester or phenoxyethanol; and the soothing agent is allantoin.

[0009] In one embodiment, the polygala root extract is prepared by the following steps:

[0010] The stems and leaves of Polygala tenuifolia are freeze-dried and then crushed, and extracted in a mixed solvent of ethanol and water; the extract is concentrated, butanediol is added and mixed, and the ethanol is removed to obtain the Polygala tenuifolia extract.

[0011] In one embodiment, the mass ratio of ethanol to water is 1:(0.8-1.2); the mass ratio of Polygala tenuifolia stems and leaves to the solvent is 1:(1-5); and the extraction time is 12-36 hours.

[0012] In one embodiment, the extraction process is: after the stems and leaves of Polygala tenuifolia are extracted once, the extract is poured out to obtain a first extract; the residue is added with a solvent and then extracted a second time to obtain a second extract; the first extract and the second extract are combined to obtain an extract.

[0013] In one embodiment, the extract is first filtered through a filter screen, and the filtrate is concentrated by vacuum distillation at 60° C.; when the filtrate is evaporated to 10% remaining, butanediol is added in an amount equal to the filtrate, and stirred to obtain a mixed solution;

[0014] The mixed solution was vacuum distilled at 50° C. to remove ethanol, thereby obtaining the polygala root extract.

[0015] In a second aspect, the present invention provides a method for preparing the antioxidant composition, comprising the following steps:

[0016] S1: Mix the additive components, heat them, homogenize them, and keep them warm to obtain an additive mixture;

[0017] S2: Cooling the auxiliary agent mixture, adding Polygala tenuifolia extract and isopentyl glycol to obtain the antioxidant composition.

[0018] In one embodiment, in step S1, the temperature is raised to 80-85°C and kept warm for 15-25 minutes; and in step S2, the temperature is lowered to 30-45°C.

[0019] In a third aspect, the embodiments of the present application provide the use of the antioxidant composition in skin care products, which include water-based skin care products, gel-based skin care products, emulsion-based skin care products, or cream-based skin care products.

[0020] The advantages or beneficial effects of the above technical solution include at least:

[0021] The present invention discloses an antioxidant composition containing a Polygala root extract, comprising the Polygala root extract and isopentyl glycol. The flavonoids contained in the Polygala root extract can inhibit oxidation reactions and effectively resist aging. Isopentyl glycol can temporarily open the lipids between the skin's stratum corneum, thereby allowing more water-soluble components that are difficult to enter to enter the skin, improving the effectiveness of the Polygala root extract in penetrating the stratum corneum and effectively enhancing the antioxidant capacity of the Polygala root extract. The present invention also discloses a method for preparing the Polygala root extract, which avoids the waste of natural resources, improves the utilization rate of the Polygala root stems and leaves, and achieves greater economic benefits.

[0022] The above summary is for illustrative purposes only and is not intended to be limiting in any way. In addition to the illustrative aspects, embodiments and features described above, further aspects, embodiments and features of the present application will be readily apparent by reference to the following detailed description. DETAILED DESCRIPTION

[0023] Hereinafter, only certain exemplary embodiments are briefly described. As will be appreciated by those skilled in the art, the described embodiments may be modified in various ways without departing from the spirit or scope of the present application. Therefore, the description is considered to be illustrative and non-restrictive in nature.

[0024] The present invention provides an antioxidant composition containing a polygala root extract, comprising the following components in parts by weight:

[0025] Polygala tenuifolia extract: 0.5-2 parts; isopentyl glycol: 0.5-2 parts; adjuvant: 4.41-24.1 parts; solvent: 74.9-91.59 parts.

[0026] The main ingredients of the antioxidant composition of the present application are polygala extract and isopentyl glycol, etc., which have no side effects on the human body and can be suitable for all kinds of people. The natural ingredients contained therein each have the effect of improving skin problems. Compared with various antioxidant skin care products currently on the market, the skin care products containing the antioxidant composition provided by this solution not only have extremely small side effects on the human body but also have significant antioxidant effects.

[0027] The antioxidant mechanism is achieved through the following pathways: the flavonoids contained in the Polygala extract act as free radical traps, reacting with free radicals in the body to generate phenoloxyl radicals. Due to the stable P-π conjugated structure, this can effectively inhibit the chain reaction of free radicals. Metal ions in the human body generally act as catalysts for oxidation reactions, and the adjacent hydroxyl or ketone groups on the flavonoids can chelate these metal ions, effectively inhibiting the oxidation reaction. The flavonoids contained in the Polygala extract can also reduce some other antioxidants (such as vitamins), allowing them to function again, thereby enhancing antioxidant capacity and effectively resisting aging.

[0028] Isopentyldiol is an amphiphilic molecular structure with good skin affinity. It can temporarily open the lipids between the stratum corneum of the skin, thereby allowing more water-soluble components that are difficult to enter to enter the skin, thereby improving the penetration effect of Polygala tenuifolia extract into the stratum corneum and effectively improving the antioxidant capacity of Polygala tenuifolia extract.

[0029] As one embodiment, the auxiliary agent is one or a combination of two or more of a moisturizer, a stabilizer, a thickener, a preservative or a soothing agent. The antioxidant composition further comprises an auxiliary agent to improve the performance of the antioxidant composition or to impart certain properties to the antioxidant composition.

[0030] As one embodiment, the moisturizer includes one or a combination of two or more of propylene glycol, butylene glycol or glycerin; the stabilizer is EDTA-disodium; the thickener is xanthan gum and / or sodium polyacrylate; the preservative is one or a combination of two or more of chlorphenesin, methyl ester or phenoxyethanol; and the soothing agent is allantoin.

[0031] As one embodiment thereof, the polygala root extract is prepared by the following steps:

[0032] The stems and leaves of Polygala tenuifolia are freeze-dried and then crushed, and extracted in a mixed solvent of ethanol and water; the extract is concentrated, butanediol is added and mixed, and the ethanol is removed to obtain the Polygala tenuifolia extract.

[0033] The method for preparing the polygala root extract of the present application avoids the waste of natural resources, can improve the utilization rate of the polygala root stems and leaves, and obtain greater economic benefits.

[0034] The mass ratio of the ethanol to water is 1:(0.8-1.2); preferably, the mass ratio of the ethanol to water is 1:1.

[0035] As one embodiment, the mass ratio of Polygala tenuifolia stems and leaves to the solvent is 1:(1-5); and the extraction time is 12-36 hours.

[0036] As one embodiment, the extraction process is: after the stems and leaves of Polygala tenuifolia are extracted once, the extract is poured out to obtain a first extract; the residue is added with a solvent and then extracted a second time to obtain a second extract; the first extract and the second extract are combined to obtain an extract.

[0037] As one embodiment, the extract is first filtered through a filter, and the filtrate is concentrated by vacuum distillation at 60°C; when the filtrate is evaporated to 10% remaining, butanediol is added in an amount equal to the filtrate, and stirred to obtain a mixed solution;

[0038] The mixed solution was vacuum distilled at 50° C. to remove ethanol, thereby obtaining the polygala root extract.

[0039] The present application also provides a method for preparing the antioxidant composition, comprising the following steps:

[0040] S1: Mix the additive components, heat them, homogenize them, and keep them warm to obtain an additive mixture;

[0041] S2: Cooling the auxiliary agent mixture, adding Polygala tenuifolia extract and isopentyl glycol to obtain the antioxidant composition.

[0042] The present invention adds Polygala root extract and isopentyl glycol to an antioxidant composition. The flavonoids contained in the Polygala root extract react with free radicals in the body to generate phenoloxyl radicals. Due to the stable Pπ conjugated structure, the chain reaction of free radicals can be effectively inhibited. At the same time, the adjacent hydroxyl or ketone groups on the flavonoids can chelate metal ions that act as catalysts for oxidation reactions in the human body, effectively inhibiting the oxidation reaction. The flavonoids contained in the Polygala root extract can also reduce some other antioxidants (such as vitamins), allowing them to resume their function, thereby enhancing antioxidant capacity and effectively resisting aging. Isopentyl glycol can significantly scavenge reactive oxygen free radicals produced by cells, effectively improving antioxidant capacity.

[0043] As one embodiment, in step S1, the temperature is raised to 80-85°C and kept warm for 15-25 minutes; and in step S2, the temperature is lowered to 30-45°C.

[0044] The present application also provides the use of the antioxidant composition in skin care products, which include water-based skin care products, gel-based skin care products, emulsion-based skin care products or cream-based skin care products.

[0045] In this embodiment, the antioxidant composition can be applied to various skin care products, so that the skin care products containing the antioxidant composition provided by the present invention have antioxidant capacity and are effective in resisting aging.

[0046] The following is a further description of the specific implementation methods.

[0047] Example 1

[0048] Step 1: Rinse the sorted Polygala stems and leaves, spread them out on a sterilized stainless steel tray, and freeze-dry for 20 hours;

[0049] Step 2: Grind the freeze-dried Polygala tenuifolia stems and leaves in step 1 with a grinder and sieve with a 50-mesh sieve;

[0050] Step 3: Add the stems and leaves of Polygala tenuifolia, 95% ethanol and deionized water in a weight ratio of 1:1:1 into an extraction tank, stir at low speed, and extract for 24 hours. Pour out the first crude extract, leave the residue in the tank and repeat the steps of the first crude extract. After 24 hours of extraction, pour out the second crude extract, and combine the first and second crude extracts to obtain an extract;

[0051] Step 4: Filter the extract through a 200-mesh filter. After filtration, transfer the filtrate to a rotary evaporator and evacuate the filtrate. Set the temperature to 60°C for low-temperature distillation. When the filtrate is evaporated to 10%, add butanediol in an amount equal to the filtrate and stir evenly to obtain a mixed solution.

[0052] Step 5: Transfer the mixed solution to a rotary evaporator in batches, evacuate the mixture, set the temperature to 50° C., and continue to evaporate the remaining ethanol to obtain the Polygala tenuifolia extract.

[0053] Example 2

[0054] Step 1: Rinse the sorted Polygala stems and leaves, spread them out on a sterilized stainless steel tray, and freeze-dry for 20 hours;

[0055] Step 2: Grind the freeze-dried Polygala tenuifolia stems and leaves in step 1 with a grinder and sieve with a 50-mesh sieve;

[0056] Step 3: Add the stems and leaves of Polygala tenuifolia, 95% ethanol and deionized water in a weight ratio of 0.8:1:1.2 to an extraction tank, stir at low speed, and extract for 24 hours. Pour out the first crude extract, leave the residue in the tank and repeat the steps of the first crude extract. After 24 hours of extraction, pour out the second crude extract, and combine the first and second crude extracts to obtain an extract;

[0057] Step 4: Filter the extract through a 200-mesh filter. After filtration, transfer the filtrate to a rotary evaporator and evacuate the filtrate. Set the temperature to 60°C for low-temperature distillation. When the filtrate is evaporated to 10%, add butanediol in an amount equal to the filtrate and stir evenly to obtain a mixed solution.

[0058] Step 5: Transfer the mixed solution to a rotary evaporator in batches, evacuate the mixture, set the temperature to 50° C., and continue to evaporate the remaining ethanol to obtain the Polygala tenuifolia extract.

[0059] According to Table 1, the Polygala tenuifolia extract prepared in Example 1 was formulated into an antioxidant composition containing the Polygala tenuifolia extract:

[0060] Table 1

[0061]

[0062]

[0063] The preparation method of the antioxidant composition containing the polygala root extract is to prepare the composition according to the following steps using the proportions shown in Table 2:

[0064] S1: Mix propylene glycol, disodium EDTA, xanthan gum, sodium polyacrylate, chlorphenesin, methyl ester, glycerin, butylene glycol, and allantoin in the additives and heat to 80-85℃, homogenize and keep warm for 20 minutes;

[0065] S2: Cooling the auxiliary agent to below 45° C., adding the Polygala tenuifolia extract and isopentyl glycol to obtain an antioxidant composition containing the Polygala tenuifolia extract.

[0066] The preparation methods of the examples and comparative examples are the same.

[0067] 1. According to QB / T2660-2004, the physical and chemical indexes of the antioxidant composition examples and comparative examples prepared above were tested. The results are as follows:

[0068] (1) Appearance: Light yellow viscous liquid.

[0069] (2) Color: light yellow.

[0070] (3) Odor: Slight characteristic odor.

[0071] (4) pH value: 4.0-8.5.

[0072] (5) Heat stability test: Keep at (40±1)℃ for 24h. After returning to room temperature, there is no obvious difference in properties compared with before the test, which is qualified.

[0073] (6) Cold stability test: Keep at (5±1)℃ for 24h. After returning to room temperature, there is no obvious difference in properties compared with before the test, which is qualified.

[0074] In summary, within the range of raw material dosage designed in the formula, all have passed the stability test and are in compliance with relevant national standards.

[0075] 2. Product safety test:

[0076] The antioxidant compositions prepared in the examples and comparative examples were subjected to human patch tests according to the method described in the "Technical Specifications for Safety of Cosmetics (2015 Edition)".

[0077] Experimental population: A total of 36 subjects, 30 females and 6 males, aged 21-59, 6 in each group, meeting the volunteer inclusion criteria;

[0078] Test method: Select an area not exceeding 50mm 2 , a suitable patch tester with a depth of about 1mm, and using the closed patch test method, add about 0.020-0.025ml of the test substance into the patch tester; apply the patch tester to the curved side of the subject's forearm, remove the test substance after 24 hours, and observe the skin reaction 0.5h, 24h, and 48h after removal, and record the results according to the skin reaction grading standard in the "Technical Specifications for Safety of Cosmetics (2015 Edition)".

[0079] Test results: 36 subjects scored 0 points (negative reaction) at three observation time points: 0.5h, 24h, and 48h after removing the patch.

[0080] 3. Product efficacy test:

[0081] The examples and comparative examples prepared above were subjected to efficacy tests, including a skin photodamage repair test and a skin inflammation relief test.

[0082] 1. Skin photodamage repair test:

[0083] Testing Principle: UV imaging can penetrate the epidermis and capture UV-induced pigmentation (a sign of free radical oxidative damage) deep within the skin. Antioxidants scavenge free radicals and inhibit melanin synthesis, resulting in a reduction in the number or size of UV-affected spots.

[0084] Test steps:

[0085] (1) Take the cosmetics of Examples 3-5 and Comparative Examples 1-3.

[0086] (2) The subject should thoroughly cleanse their face and remove makeup and skin care products to avoid interference with imaging.

[0087] (3) A technician used a VISIA skin detector to measure the UV spots on the skin of the subjects before applying the cosmetics of the examples and comparative examples.

[0088] (4) After the subjects used the cosmetics of the examples or comparative examples for four consecutive weeks, the skin UV spots (UV Spots) data were measured using a VISIA skin detector.

[0089] (5) The values ​​measured on the subjects' skin each time were counted and the changing patterns of their skin UV spots were analyzed to determine the efficacy of the cosmetics of the embodiments and comparative examples in improving skin UV spots (the same technician performed the measurements on each subject each time).

[0090] 2. Skin inflammation relief test

[0091] Test Principle: Cross-polarized light is used to detect subcutaneous capillary dilation and inflammation (oxidative stress often causes redness). When antioxidants reduce inflammation, the red area shrinks and hemoglobin concentrations decrease.

[0092] Test steps:

[0093] (1) Take the cosmetics of Examples 3-5 and Comparative Examples 1-3.

[0094] (2) The subject should thoroughly cleanse their face and remove makeup and skin care products to avoid interference with imaging.

[0095] (3) A technician used a VISIA skin tester to measure the red areas of the subjects' skin before applying the cosmetics of the examples and comparative examples.

[0096] (4) After the subjects used the cosmetics of the examples or comparative examples for four consecutive weeks, the red areas of the skin were measured using a VISIA skin detector.

[0097] (5) The numerical values ​​measured on the subjects' skin each time were counted and the changing patterns of their skin red areas were analyzed to determine the efficacy of the cosmetics of the Examples and Comparative Examples in improving the skin red areas (the same technician performed the measurements on each subject each time).

[0098] Test group: 60 wrinkle-bearing individuals with an average age of 40-50 years old. Among them, 20 were male and 40 were female, with healthy skin. Ten subjects were included in each example and comparative example.

[0099] After using the above products for four weeks (28 days), the statistical data are shown in Tables 3 and 4.

[0100] Table 3 Statistics of skin UV spots test data

[0101]

[0102] Table 4 Statistics of skin red area test data

[0103]

[0104]

[0105] The examples are prepared by adding different proportions of Polygala root extract and isopentyl glycol; Comparative Example 1 only adds Polygala root extract, Comparative Example 2 only adds isopentyl glycol, and Comparative Example 3 is a blank control. As shown in Tables 3 and 4, the test results show that after 4 weeks of use, the subjects using Comparative Examples 1-3 showed moderate improvement in skin elasticity and texture, while the subjects using Examples 3-5 showed significant improvement in skin elasticity and texture after 4 weeks of use, indicating that the antioxidant effects of the example products with different proportions of Polygala root extract and isopentyl glycol are significant. After analysis of instrumental test data, skin elasticity and texture were significantly improved after 4 weeks of use, indicating that the synergistic effect of the Polygala root extract combined with isopentyl glycol products is significant, significantly better than using Polygala root extract or isopentyl glycol alone.

[0106] In the description of this specification, the reference terms "one embodiment," "some embodiments," "example," "specific example," or "some examples" mean that the specific features, structures, materials, or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present application. Moreover, the specific features, structures, materials, or characteristics described may be combined in any appropriate manner in any one or more embodiments or examples. In addition, those skilled in the art may combine and combine different embodiments or examples described in this specification, as well as features of different embodiments or examples, unless they are mutually inconsistent.

[0107] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features being referred to. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. Throughout the description of this application, "plurality" means two or more, unless otherwise specifically defined.

[0108] The above description is merely a specific embodiment of the present application, but the scope of protection of the present application is not limited thereto. Any person skilled in the art can easily conceive of various modifications or substitutions within the technical scope disclosed in this application, and such modifications or substitutions should be included within the scope of protection of the present application. Therefore, the scope of protection of the present application should be based on the scope of protection of the claims.

Claims

1. An antioxidant composition containing a Polygala tenuifolia extract, characterized in that: It comprises the following components in parts by weight: Polygala root extract: 0.5-2 parts; Isopentyldiol: 0.5-2 parts; Adjuvant: 4.41-24.1 parts; Solvent: 74.9-91.59 parts.

2. The antioxidant composition containing the Polygala tenuifolia extract according to claim 1, wherein The auxiliary agent is one or a combination of two or more of a moisturizer, a stabilizer, a thickener, a preservative or a soothing agent.

3. The antioxidant composition containing the Polygala tenuifolia extract according to claim 2, wherein The moisturizer includes one or a combination of two or more of propylene glycol, butylene glycol or glycerin; the stabilizer is disodium EDTA; the thickener is xanthan gum and / or sodium polyacrylate; the preservative is one or a combination of two or more of chlorphenesin, methyl ester or phenoxyethanol; and the allergy-reducing agent is allantoin.

4. The antioxidant composition containing the Polygala tenuifolia extract according to claim 2, wherein The polygala root extract is prepared by the following steps: The stems and leaves of Polygala tenuifolia are freeze-dried and then crushed, and extracted in a mixed solvent of ethanol and water; the extract is concentrated, butanediol is added and mixed, and the ethanol is removed to obtain the Polygala tenuifolia extract.

5. The antioxidant composition containing the Polygala tenuifolia extract according to claim 4, wherein The mass ratio of the ethanol to the water is 1:(0.8-1.2); the mass ratio of the polygala stems and leaves to the solvent is 1:(1-5); and the extraction time is 12-36 hours.

6. The antioxidant composition containing the Polygala tenuifolia extract according to claim 4, wherein The extraction process is as follows: after the stems and leaves of Polygala tenuifolia are extracted once, the extract is poured out to obtain a first extract; the residue is added with a solvent and then extracted for a second time to obtain a second extract; and the first extract and the second extract are combined to obtain an extract.

7. The antioxidant composition containing the Polygala tenuifolia extract according to claim 4, wherein The extract is first filtered through a filter, and the filtrate is concentrated by vacuum distillation at 60°C; when the filtrate is evaporated to 10% remaining, butanediol is added in an amount equal to the filtrate, and stirred evenly to obtain a mixed solution; The mixed solution was vacuum distilled at 50° C. to remove ethanol, thereby obtaining the polygala root extract.

8. The method for preparing the antioxidant composition according to any one of claims 1 to 7, characterized in that: The following steps are involved: S1: Mix the additive components, heat them, homogenize them, and keep them warm to obtain an additive mixture; S2: Cooling the auxiliary agent mixture, adding Polygala tenuifolia extract and isopentyl glycol to obtain the antioxidant composition.

9. The method for preparing the antioxidant composition according to claim 8, wherein: In step S1, the temperature is raised to 80-85°C and kept warm for 15-25 minutes; in step S2, the temperature is lowered to 30-45°C.

10. Use of the antioxidant composition according to any one of claims 1 to 7 in skin care products, wherein the skin care products include water-based skin care products, gel-based skin care products, emulsion-based skin care products or cream-based skin care products.

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