A ship sewage treatment system and method

Abstract

This invention relates to the field of ship wastewater treatment technology, specifically to a ship wastewater treatment system and method. The system includes an intelligent sensing module composed of a quantum tunneling sensor array and a live microbial sensor for real-time detection of heavy metals, pathogens, and chemical oxygen demand (COD) in wastewater; a plasma-sonic-chemical synergistic reactor configured to remove suspended solids and organic pollutants through the synergistic effect of magnetic nanoparticle adsorption and sonochemical oxidation; a synthetic microbial biomimetic reactor with built-in gene-edited bacterial communities and 3D-printed porous carriers for degrading organic matter and removing nitrogen and phosphorus; a membrane separation and resource recovery module comprising a biomimetic cell membrane intelligent filtration system and an electrochemical concentration unit for freshwater resource recovery and phosphate and lithium-ion recovery; an energy recovery module consisting of a photocatalytic-microbial fuel cell to achieve simultaneous end-of-pipe treatment of pollutants and power generation; and an intelligent control module.

Description

Technical Field

[0001] This invention relates to the field of ship sewage treatment technology, and specifically to a ship sewage treatment system and method. Background Technology

[0002] With the rapid development of the global shipping industry, wastewater generated by ships has caused serious pollution problems to the marine environment. Traditional wastewater treatment methods have many shortcomings in treating complex water qualities and preventing the spread of pathogens, making it difficult to meet increasingly stringent environmental protection requirements. Specifically, existing technologies face the following major challenges:

[0003] Traditional wastewater treatment methods typically rely on chemical precipitation and filtration to remove heavy metals and pathogens, but these methods are often inefficient and prone to causing secondary pollution.

[0004] Traditional biological treatment methods have limited effectiveness in degrading complex organic pollutants (such as petroleum hydrocarbons), especially in high salinity or low temperature environments where microbial activity is inhibited.

[0005] While traditional membrane separation technology can effectively separate fresh water and concentrate, it generally suffers from problems such as low flux, high energy consumption, and easy clogging, resulting in high processing costs.

[0006] Traditional wastewater treatment processes are energy-intensive and lack effective energy recovery mechanisms, leading to increased operating costs and hindering sustainable development.

[0007] Traditional wastewater treatment systems lack intelligent monitoring capabilities and cannot respond to changes in water quality in real time, resulting in unstable treatment effects and high maintenance costs.

[0008] Traditional oil spill detection methods, such as gas chromatography-mass spectrometry (GC-MS), while highly accurate, suffer from bulky equipment and lengthy processing times, making them unsuitable for rapid online monitoring on ships. Conventional fluorescence or electrochemical sensors are susceptible to interference from complex seawater matrices (such as high salinity, humic acid, and suspended solids), and lack specificity and sensitivity for low-concentration, multi-component mixed oil spills. In particular, current technologies lack an integrated sensing solution capable of simultaneously identifying the chemical components of oil spills and their biotoxic effects (such as whether they induce drug resistance genes in pathogens). Summary of the Invention

[0009] To address the shortcomings of existing technologies, this invention provides a ship wastewater treatment system and method that integrates intelligent sensing, multi-stage treatment, membrane separation and resource recovery, and energy recovery functions. This not only solves many technical problems existing in traditional methods, but also achieves the goal of efficient, low-cost, and environmentally friendly wastewater treatment.

[0010] According to a first aspect of the present invention, a ship sewage treatment system is provided, comprising:

[0011] Intelligent sensing module: Composed of a quantum tunneling sensor array and a live microbial sensor, used to detect heavy metals, pathogens and chemical oxygen demand (COD) in wastewater in real time;

[0012] Multi-level processing module:

[0013] Plasma-sonic chemical synergistic reactor: configured to remove suspended solids and organic pollutants through the synergistic effect of magnetic nanoparticle adsorption and sonochemical oxidation;

[0014] Synthetic microbial biomimetic reactor: It has built-in gene-edited bacterial communities and 3D-printed porous carriers for degrading organic matter and removing nitrogen and phosphorus;

[0015] Membrane separation and resource recovery module: includes a biomimetic cell membrane intelligent filtration system and an electrochemical concentration unit, used for freshwater resource recovery and phosphate and lithium ion recovery;

[0016] Energy recovery module: Composed of photocatalytic-microbial fuel cell, it realizes the simultaneous generation of electricity and end-of-pipe treatment of pollutants;

[0017] Intelligent control module: Dynamically optimizes the operating parameters of each unit through a digital twin platform to ensure processing efficiency and energy balance.

[0018] This invention, by introducing a quantum tunneling sensor array (graphene quantum dots with a surface-modified CRISPR-Cas12a system) and a live microbial sensor (a mutant of Vibrio fischeri expressing the lux gene), enables real-time detection and precise control of heavy metal and pathogen concentrations. Combining the synergistic effect of magnetic nanoparticle adsorption and sonochemical oxidation, it can efficiently remove suspended solids and organic pollutants, ensuring water quality safety.

[0019] This invention employs a synthetic microbial biomimetic reactor, incorporating gene-edited bacterial communities (such as *Bocomella aracanno* expressing cytochrome P450 enzymes and *Pseudomonas schrenckii* carrying autotrophic denitrification gene clusters), enabling efficient degradation of organic pollutants under various environments and achieving nitrogen and phosphorus removal. The design of the 3D-printed porous carrier further enhances the adhesion and metabolic efficiency of the microorganisms.

[0020] The biomimetic cell membrane intelligent filtration system of this invention utilizes a lipid bilayer membrane formed by the self-assembly of aquaporin-Z and dipalmitoylphosphatidylcholine (DPPC), combined with a carbon nanotube and polyamide hybrid matrix support layer, achieving high flux (≥100 LMH / bar) and high rejection rate (NaCl rejection rate ≥99.9%). An electrochemical concentration unit is used to recover phosphate and lithium ions, further improving resource utilization.

[0021] The energy recovery module of this invention consists of a photocatalytic-microbial fuel cell, which not only achieves end-of-pipe treatment of pollutants but also simultaneously generates electricity (system net capacity ≥ 0.4 kWh / m³). 3 This significantly reduces overall energy consumption. The application of the digital twin platform ensures dynamic optimization of the operating parameters of each unit, achieving the best match between processing efficiency and energy balance.

[0022] The intelligent control module of this invention, through a digital twin platform, can monitor water quality data in real time and dynamically adjust the operating parameters of each unit. This intelligent management method not only improves processing efficiency but also reduces the need for manual intervention and lowers maintenance costs.

[0023] According to an embodiment of the present invention, in the intelligent sensing module:

[0024] The quantum tunneling sensor array is composed of graphene quantum dots (GQDs) of a surface-modified CRISPR-Cas12a system, with a detection limit of ≤0.1 ppb for heavy metal ions;

[0025] The live microbial sensor is a Vibrio fischeri mutant expressing the lux gene, and its bioluminescence intensity is linearly correlated with COD concentration in the range of 10-1000 mg / L (RL). 2 ≥0.99).

[0026] The Vibrio fischeri mutant was designed to detect chemical oxygen demand (COD), and its working principle is based on the relationship between the bioluminescence intensity of this strain and the organic matter content in water samples. The Vibrio fischeri mutant expressing the lux gene exhibits a corresponding bioluminescent response upon contact with samples containing a certain concentration of organic matter. The intensity of this response is linearly correlated with the COD in the sample, and within the range of 10-1000 mg / L, Rlux... 2 With a value of ≥0.99, it can reflect water quality very accurately.

[0027] When this mutant is exposed to a water sample, if biodegradable organic matter is present, this matter will affect the bacterial metabolic rate, thereby affecting the expression level of the lux gene and leading to changes in luminescence intensity. By quantifying these changes, the COD value of the water sample can be indirectly determined.

[0028] According to an embodiment of the present invention, the plasma-sonic chemical co-reactor comprises:

[0029] Fe3O4@ZIF-8 core-shell magnetic nanoparticles with a particle size of 50-100 nm and an addition amount of 0.5-1 g / L;

[0030] The ultrasonic generator operates at a frequency of 1MHz ± 5% and a power density of 50W / cm². 2 ;

[0031] The pulsed plasma device has an output voltage of 20kV±5% and a pulse width of 100ns±10%.

[0032] According to an embodiment of the present invention, in the synthetic microbial biomimetic reactor:

[0033] The gene-edited microbial community includes:

[0034] Alcanivorax borkumensis, which expresses cytochrome P450 enzymes, is used for the degradation of petroleum hydrocarbons;

[0035] Pseudomonas stutzeri, carrying an autotrophic denitrification gene cluster, is used for nitrate reduction;

[0036] The 3D printed porous carrier is a polylactic acid-glycolic acid copolymer (PLGA) porous scaffold with a surface pore size of 50±5μm, an internal pore size of 10±2μm, and a porosity of ≥90%.

[0037] According to an embodiment of the present invention, the biomimetic cell membrane intelligent filtration system includes:

[0038] The active layer is composed of a lipid bilayer membrane formed by the self-assembly of aquaporin-Z protein and dipalmitoylphosphatidylcholine (DPPC);

[0039] The support layer is a hybrid matrix of carbon nanotubes and polyamide, with a thickness of ≤200nm and an operating pressure of 0.5-1.5bar;

[0040] The biomimetic cell membrane intelligent filtration system has a water flux ≥100 LMH / bar and a NaCl retention rate ≥99.9%. Aquaporin-Z was used as part of the construction of this biomimetic cell membrane intelligent filtration system, which mimics the biofilm mechanisms found in nature to achieve efficient water treatment and resource recovery.

[0041] Aquaporin-Z self-assembles with dipalmitoylphosphatidylcholine (DPPC) to form a lipid bilayer membrane, which can create a highly efficient selective permeation membrane for freshwater separation and concentrate treatment in wastewater treatment processes.

[0042] According to an embodiment of the present invention, the CRISPR-Cas12a system is a CdSe / ZnS core-shell quantum dot structure immobilized with a CRISPR-Cas12a complex and an oil-specific DNA aptamer.

[0043] When the sensor identifies the target pollutant, it triggers the Cas12a trans-cleavage activity, resulting in amplification of the quantum dot fluorescence signal, with a detection limit of 0.01 ppm for diesel.

[0044] According to an embodiment of the present invention, the particle size of the CdSe / ZnS core-shell structured quantum dots is 2-5 nm;

[0045] The CdSe / ZnS core-shell quantum dots are immobilized on the surface via a carboxyl-amino coupling reaction.

[0046] CRISPR-Cas12a complex and oil-specific DNA aptamers.

[0047] CdSe / ZnS core-shell quantum dots exhibit high fluorescence quantum yields, providing strong and stable fluorescence signals even at low concentrations. A CRISPR-Cas12a complex and an oil-specific DNA aptamer were immobilized on the quantum dot surface via a carboxyl-amino coupling reaction. This surface modification method not only enhances the stability of the quantum dots but also endows them with specific recognition capabilities.

[0048] CRISPR-Cas12a complex: After recognizing a specific target sequence, the Cas12a protein triggers its trans-cleavage activity, that is, non-specifically cleaving the surrounding single-stranded DNA.

[0049] Oil-specific DNA aptamers: These aptamers can specifically bind to oil pollutants such as diesel fuel, enabling the Cas12a complex to be activated in the presence of the target pollutant.

[0050] The water sample to be tested is introduced into a detection system containing the aforementioned quantum dot sensor. If the water sample contains oil pollutants such as diesel fuel, oil-specific DNA adaptors will bind to them, forming a complex. The formed complex activates the Cas12a protein, triggering its trans-cleavage activity. Cas12a non-specifically cleaves the fluorescently labeled DNA probes attached to the surrounding quantum dots, resulting in a significant enhancement of the fluorescence signal of the quantum dots. By measuring the change in fluorescence signal, the concentration of oil in the water is calculated and compared with a set standard value to determine whether further treatment is needed.

[0051] When oily contaminants such as diesel fuel are present in ship sewage, their specific components (e.g., polycyclic aromatic hydrocarbons like pyrene and benzo[a]pyrene) bind with high affinity to DNA aptamers with unique three-dimensional structures, causing the aptamers to transform from a random coil conformation to a rigid complex structure such as a G-quadruplex. This conformationally changed DNA aptamer-oil contaminant complex acts as a highly efficient activator, binding to the Cas12a / crRNA binary complex and inducing a conformational change in the Cas12a protein, exposing its trans-cleavage catalytic center. The activated Cas12a protein indiscriminately cleaves all single-stranded DNA (ssDNA) in the reaction system. In this invention, a large number of ssDNA reporter molecules labeled with a fluorescence quencher group (BHQ-1) are pre-immobilized on the surface of CdSe / ZnS quantum dots using a streptavidin-biotin system. The trans-cleavage activity of Cas12a cleaves these reporter molecules, causing the quencher group to move away from the quantum dot surface, thereby relieving the fluorescence resonance energy transfer (FRET) effect and leading to an exponential recovery and enhancement of the quantum dot's fluorescence signal.

[0052] When the sensor detects a target contaminant (such as diesel fuel), oil-specific DNA aptamers bind to the contaminant, triggering the trans-cleavage activity of Cas12a. Cas12a non-specifically cleaves the fluorescently labeled DNA probe attached to the quantum dot surface, resulting in a significant enhancement of the quantum dot's fluorescence signal. Due to this signal amplification effect, the sensor's detection limit for diesel fuel can reach 0.01 ppm (parts per million), far exceeding the sensitivity of traditional detection methods.

[0053] In ship wastewater treatment systems, this sensor can monitor the oil content in wastewater in real time, ensuring that the treated water meets environmental standards. It can also be used for marine environmental monitoring, quickly and accurately detecting oil pollution in water bodies and enabling timely response measures.

[0054] According to an embodiment of the present invention, the electrochemical concentration unit employs an IrO2-Ta2O5 / Ti anode and a nitrogen-doped graphene cathode.

[0055] The anode, coated with iridium oxide and tantalum oxide, exhibits excellent electrocatalytic activity and stability, enabling long-term operation at high current densities without significant degradation. Titanium, as the substrate material, provides not only good mechanical strength but also excellent corrosion resistance, making it suitable for harsh environments such as strong acids and alkalis.

[0056] The cathode uses nitrogen-doped graphene. Doping nitrogen atoms into the graphene structure introduces more active sites, enhancing electrocatalytic activity, particularly in reduction reactions. Furthermore, nitrogen doping improves the conductivity and hydrophilicity of graphene, promoting ion transport in the electrolyte.

[0057] According to a second aspect of the present invention, a method for treating ship sewage is provided, employing the above-described ship sewage treatment system, comprising the following steps:

[0058] The concentration of pollutants in wastewater is detected by an intelligent sensing module, and the data is transmitted to the intelligent control module in real time.

[0059] In a plasma-sonochemistry co-processing reactor, heavy metals are adsorbed by magnetic nanoparticles, while organic matter is degraded by reactive oxygen species generated by ultrasonic cavitation and plasma activation.

[0060] In the synthetic microbial biomimetic reactor, biodegradation was carried out by gene-edited microbial communities, with the hydraulic retention time (HRT) controlled at 4 ± 0.5 hours;

[0061] Fresh water and concentrate are separated by a biomimetic cell membrane intelligent filtration system. The fresh water is reused, and the concentrate enters the electrochemical unit to recover phosphate and lithium ions.

[0062] Residual pollutants are treated and converted into electrical energy using a photocatalytic-microbial fuel cell, with a net system capacity of ≥0.4 kWh / m³. 3 ;

[0063] By dynamically adjusting the parameters of each module through a digital twin platform, the quality of discharged water is reduced to 50% below the limits set by the International Maritime Organization (IMO).

[0064] According to an embodiment of the present invention, the recovery rate of the magnetic nanoparticles is ≥98%, and they are regenerated by 0.1M citric acid solution after recovery;

[0065] During the sonochemical oxidation stage, the pH value is maintained at 3-5 by an automatic dosing system, and the concentration of hydroxyl radicals (·OH) is ≥1×10⁻⁶. -4 mol / L.

[0066] According to an embodiment of the present invention, in step (4):

[0067] Current density is 5-20 mA / cm 2 ;

[0068] Phosphate recovery rate ≥95%, lithium ion recovery rate ≥90%.

[0069] The wastewater treatment system and method provided by this invention efficiently remove heavy metals and pathogens through advanced sensing technology and synergistic treatment methods, ensuring water quality safety. Gene-edited microbial communities and 3D-printed porous carriers are used to efficiently degrade organic pollutants, improving biodegradation efficiency. A biomimetic cell membrane intelligent filtration system and electrochemical concentration unit are employed to achieve efficient water resource recovery and recycling. A photocatalytic-microbial fuel cell is used to achieve pollutant treatment and energy recovery, reducing overall energy consumption. A digital twin platform is applied to achieve real-time monitoring and dynamic adjustment, improving system stability and operability.

[0070] This invention creatively constructs a 'chemical-biological' dual-mode sensing interface based on the synergistic amplification of CRISPR-Cas12a trans-cleavage activity and quantum dot energy transfer effect. This interface is not a simple superposition of CRISPR and quantum dots, but rather achieves highly specific recognition and signal conversion of oil molecules through ingenious molecular design, ultimately realizing ultrasensitive detection through the significant enhancement of quantum dot fluorescence signal.

[0071] This invention provides a ship sewage treatment system and method that integrates multiple advanced technologies, which not only solves many technical problems existing in traditional methods, but also achieves the goal of efficient, low-cost and environmentally friendly sewage treatment. Detailed Implementation

[0072] This application provides a complete ship sewage treatment process based on a ship sewage treatment system and method.

[0073] Example 1

[0074] The system includes an intelligent sensing module, a multi-level processing module, a membrane separation and resource recovery module, an energy recovery module, and an intelligent control module.

[0075] 1. Intelligent Sensing Module

[0076] Sensor installation and calibration:

[0077] A quantum tunneling sensor array (graphene quantum dots GQDs with surface-modified CRISPR-Cas12a system) and a live microbial sensor (Vibrio fischeri mutant expressing the lux gene) were installed at the ship's sewage inlet.

[0078] The sensor was calibrated to ensure that the detection limit for heavy metal ions was ≤0.1 ppb and that the COD concentration detection range showed a linear correlation (R0) within the range of 10-1000 mg / L. 2 ≥0.99).

[0079] Real-time monitoring:

[0080] The sensors are activated to monitor heavy metals, pathogens, and chemical oxygen demand (COD) in wastewater in real time and transmit the data to the central control system.

[0081] The central control system adjusts the parameters of subsequent processing steps based on real-time data.

[0082] 2. Multi-level processing module

[0083] Plasma-sonic synergistic reactor:

[0084] Wastewater was introduced into a plasma-sonic-chemical co-processing reactor, and Fe3O4@ZIF-8 core-shell magnetic nanoparticles (particle size 50-100nm, dosage 0.5-1g / L) were added.

[0085] Start the ultrasonic generator (operating frequency 1MHz±5%, power density 50W / cm²). 2 ) and pulsed plasma device (output voltage 20kV±5%, pulse width 100ns±10%).

[0086] Magnetic nanoparticles are used to adsorb heavy metals in wastewater, and active oxygen species generated by ultrasonic cavitation and plasma activation degrade organic pollutants.

[0087] The recovery rate of magnetic nanoparticles is ≥98%. After recovery, they are regenerated with 0.1M citric acid solution for future use.

[0088] During the sonochemical oxidation stage, the pH value is maintained at 3-5 by an automatic dosing system, and the concentration of hydroxyl radicals (·OH) is ≥1×10⁻⁶. -4 mol / L.

[0089] Synthetic microbial biomimetic reactor:

[0090] Wastewater treated by a plasma-sonochemistry synergistic reactor is introduced into a synthetic microbial biomimetic reactor.

[0091] The reactor contains gene-edited bacterial cultures (such as A. borkumensis expressing cytochrome P450 enzyme and P. stutzeri carrying autotrophic denitrification gene clusters), which are attached to a 3D-printed PLGA porous scaffold (surface pore size 50±5μm, internal pore size 10±2μm, porosity ≥90%).

[0092] The hydraulic retention time (HRT) was controlled at 4 ± 0.5 hours, and gene-edited microbial communities were used to degrade organic matter and perform denitrification and phosphorus removal treatment.

[0093] 3. Membrane separation and resource recovery module

[0094] Bionic cell membrane intelligent filtration system:

[0095] Wastewater that has undergone biodegradation treatment is introduced into a biomimetic cell membrane intelligent filtration system.

[0096] The system consists of a lipid bilayer membrane formed by the self-assembly of Aquaporin-Z protein and dipalmitoylphosphatidylcholine (DPPC) as the active layer, and a carbon nanotube and polyamide hybrid matrix as the support layer (thickness ≤200nm, operating pressure 0.5-1.5bar).

[0097] The filtration system features high throughput (≥100 LMH / bar) and high rejection rate (NaCl rejection rate ≥99.9%), enabling the separation of fresh water and concentrate.

[0098] Electrochemical Concentration Unit:

[0099] The concentrate enters the electrochemical concentration unit, using an IrO2-Ta2O5 / Ti anode and a nitrogen-doped graphene cathode, with a current density of 5-20 mA / cm². 2 .

[0100] Under the influence of an electric field, phosphate and lithium ions migrate toward their respective electrodes and accumulate or precipitate near the electrodes.

[0101] Phosphate recovery rate ≥95%, lithium ion recovery rate ≥90%, these valuable substances are collected and stored for reuse.

[0102] 4. Energy recovery module

[0103] Photocatalytic-microbial fuel cells:

[0104] Residual pollutants are introduced into a photocatalytic-microbial fuel cell, where the synergistic effect of photocatalysts and microorganisms is used to convert the pollutants into electrical energy.

[0105] System net capacity ≥ 0.4 kWh / m 3 The generated electricity can be used to support the operation of the entire wastewater treatment system, reducing energy costs.

[0106] 5. Intelligent control module

[0107] Digital twin platform:

[0108] The digital twin platform is used to monitor the operating status and water quality parameters of each module in real time, and to dynamically optimize the operating parameters of each unit.

[0109] Through data analysis and feedback mechanisms, we ensure that the quality of discharged water is below 50% of the limits set by the International Maritime Organization (IMO).

[0110] Specific implementation steps

[0111] Startup and Preprocessing:

[0112] Install and calibrate all sensors and processing equipment to ensure the system is in optimal working condition.

[0113] Activate the intelligent sensing module to monitor various indicators in wastewater in real time.

[0114] Start all modules, warm up, and calibrate the sensors;

[0115] Input ship sewage and record initial water quality data.

[0116] The intelligent sensing module records the sensor output signals (fluorescence intensity, luminescence intensity);

[0117] The accuracy of the sensor response was verified by comparing it with the standard curve.

[0118] Output the predicted types and concentrations of pollutants.

[0119] Primary treatment: Plasma-sonochemical synergistic reaction:

[0120] Wastewater is introduced into a plasma-sonic-chemical co-process reactor, magnetic nanoparticles are added, and an ultrasonic generator and plasma device are activated.

[0121] Run the reactor and set the ultrasonic frequency (1MHz) and power density (50W / cm²). 2 ), pulsed plasma parameters (20kV, 100ns);

[0122] Add Fe3O4@ZIF-8 magnetic nanoparticles (0.5–1 g / L);

[0123] Monitor and adjust the pH value to ensure that the concentration of hydroxyl radicals meets the requirements.

[0124] Regularly sample and test the removal rates of heavy metals, COD, and petroleum substances.

[0125] The magnetic nanoparticles are recycled and regenerated for the next round of use.

[0126] Secondary treatment: Biomimetic reaction of synthetic microorganisms:

[0127] Wastewater that has undergone primary treatment is introduced into a synthetic microbial biomimetic reactor, where gene-edited microbial communities are used for biodegradation and denitrification and phosphorus removal, with the HRT controlled at 4 ± 0.5 hours; changes in ammonia nitrogen and total phosphorus are monitored.

[0128] Control the hydraulic retention time to ensure that the treatment effect achieves the expected goal.

[0129] Tertiary treatment: Membrane separation and resource recovery:

[0130] Wastewater treated by biodegradation was introduced into a biomimetic cell membrane intelligent filtration system, the operating pressure was set (0.5–1.5 bar), and the membrane flux was recorded;

[0131] Analyze the freshwater quality (pH, conductivity, NaCl rejection rate) to separate the freshwater and concentrate.

[0132] The concentrate enters the electrochemical concentration unit, and the current density is adjusted (5–20 mA / cm²). 2 Selectively recover phosphate and lithium ions, collect phosphate precipitate and lithium ion enrichment solution, and calculate recovery rate (PO43-≥95%, Li+≥90%).

[0133] Level 4 treatment: Energy recovery:

[0134] The remaining pollutants are introduced into the photocatalytic-microbial fuel cell, which operates as a photocatalytic-MFC. The open-circuit voltage and short-circuit current are measured and converted into electrical energy for the system's own use or other purposes.

[0135] Intelligent optimization and monitoring:

[0136] The system's operational status is monitored in real time through a digital twin platform, and the parameters of each module are dynamically adjusted to ensure processing efficiency and energy balance.

[0137] Regularly maintain and calibrate all sensors and processing equipment to ensure the long-term stable operation of the system.

[0138] Experimental Example

[0139] To verify the removal efficiency of a ship wastewater treatment system based on intelligent sensing, multi-stage treatment, membrane separation and resource recovery, and energy recovery for various pollutants (such as COD, BOD, heavy metals, nitrogen and phosphorus), and to evaluate its resource recovery and energy recovery capabilities.

[0140] Wastewater type: Simulated mixture of shipboard domestic sewage, oily wastewater, and a small amount of industrial wastewater. Initial water quality parameter reference values:

[0141] COD: 500–1000 mg / L

[0142] BOD5: 200–400 mg / L

[0143] Ammonia nitrogen (NH3-N): 30–60 mg / L

[0144] Total phosphorus (TP): 5–15 mg / L

[0145] Heavy metals (such as Cu) 2+ Zn 2+ Pb 2+ 0.1–1.0 mg / L

[0146] Petroleum-based substances (diesel): 5–50 ppm

[0147] After the wastewater was treated using the wastewater treatment system and method provided in Example 1, the treated wastewater was tested. The testing methods are shown in Table 1.

[0148] Table 1. Detection method in Example 1

[0149]

[0150] The wastewater treated by the system and method of Example 1 was measured using the above methods, and the results are shown in Table 2.

[0151] Table 2. Test results of Example 1

[0152] project Test Results COD removal rate ≥95％ ammonia nitrogen removal rate ≥90％ Total phosphorus removal rate ≥85％ Heavy metal removal rate ≥99％ Petroleum Removal Rate ≥98％ <![CDATA[PO43- recovery rate]]> ≥95％ <![CDATA[Li + Recovery rate ≥90％ Freshwater production rate ≥80％ System net capacity <![CDATA[≥0.4kWh / m 3 ]]> Intelligent control response time ≤5 minutes System continuous stable operation cycle ≥72 hours

[0153] The wastewater treatment system and method provided by this invention efficiently remove heavy metals and pathogens through advanced sensing technology and synergistic treatment methods, ensuring water quality safety. Gene-edited microbial communities and 3D-printed porous carriers are used to efficiently degrade organic pollutants, improving biodegradation efficiency. A biomimetic cell membrane intelligent filtration system and electrochemical concentration unit are employed to achieve efficient water resource recovery and recycling. A photocatalytic-microbial fuel cell is used to achieve pollutant treatment and energy recovery, reducing overall energy consumption. A digital twin platform is applied to achieve real-time monitoring and dynamic adjustment, improving system stability and operability.

[0154] Although preferred embodiments of the invention have been described, those skilled in the art, upon learning the basic inventive concept, can make other changes and modifications to these embodiments. Therefore, the appended claims are intended to be interpreted as including the preferred embodiments as well as all changes and modifications falling within the scope of the invention.

[0155] Obviously, those skilled in the art can make various modifications and variations to this invention without departing from its spirit and scope. Therefore, if these modifications and variations fall within the scope of the claims of this invention and their equivalents, this invention also intends to include these modifications and variations.

Claims

1. A marine sewage treatment system characterised in that, comprises, in sequence: an intelligent sensing module: composed of a quantum tunneling sensor array and a living microorganism sensor, for real-time detection of heavy metals, pathogens and chemical oxygen demand in sewage, wherein the quantum tunneling sensor array is a graphene quantum dot with a surface modified CRISPR-Cas12a system, the CRISPR-Cas12a system is a CdSe / ZnS core-shell structure quantum dot with a fixed CRISPR-Cas12a complex and oil stain specific DNA aptamer, and the living microorganism sensor is a Vibrio fischeri mutant expressing a lux gene; a multi-stage processing module, comprising: a plasma-acoustic chemical synergistic reactor: configured to remove suspended solids and organic pollutants through the synergistic effect of magnetic nanoparticle adsorption and acoustic chemical oxidation; a synthetic microorganism biomimetic reactor: with a gene editing bacterial population and a 3D printed porous carrier, for degrading organic matter and denitrifying and phosphorus removal, the gene editing bacterial population comprising: B. alacanofii expressing cytochrome P450 enzymes Alcanivorax borkumensis for degradation of petroleum hydrocarbons; Pseudomonas stutzeri carrying autotrophic denitrification gene cluster Pseudomonas stutzeri for nitrate reduction; the 3D printed porous carrier being a polylactic acid-glycolic acid copolymer porous scaffold; a membrane separation and resource recovery module: containing a biomimetic cell membrane intelligent filtration system and an electrochemical concentration unit, for freshwater resource and phosphate and lithium ion recovery; an energy recovery module: composed of a photocatalytic-microbial fuel cell, realizing end-of-pipe treatment of pollutants and synchronous generation of electrical energy; an intelligent control module: dynamically optimizing the operating parameters of each unit through a digital twin platform to ensure processing efficiency and energy balance.

2. The system of claim 1, wherein, in the intelligent sensing module: the bioluminescence intensity of the living microorganism sensor is linearly related to the COD concentration in the range of 10-1000 mg / L.

3. The system of claim 1, wherein, the plasma-acoustic chemical synergistic reactor comprises: Fe3O4@ZIF-8 core-shell structure magnetic nanoparticles with a particle size of 50-100 nm and a dosage of 0.5-1 g / L; an ultrasonic generator with a working frequency of 1 MHz±5% and a power density of 50 W / cm²; a pulsed plasma device with an output voltage of 20 kV±5% and a pulse width of 100 ns±10%.

4. The system of claim 1, wherein, in the synthetic microorganism biomimetic reactor: the 3D printed porous carrier has a surface pore size of 50±5 μm, an internal pore size of 10±2 μm, and a porosity of ≥90%.

5. The system of claim 1, wherein, the biomimetic cell membrane intelligent filtration system comprises: an active layer: composed of a lipid bilayer membrane self-assembled from aquaporin and dipalmitoyl phosphatidylcholine; a support layer: a hybrid matrix of carbon nanotubes and polyamide with a thickness of ≤200 nm and an operating pressure of 0.5-1.5 bar; the water flux of the biomimetic cell membrane intelligent filtration system is ≥100 LMH / bar.

6. The system of claim 1, wherein, when the quantum tunneling sensor array identifies the target pollutant, the Cas12a trans-cleavage activity is triggered, resulting in amplification of the quantum dot fluorescence signal, and the detection limit for diesel is 0.01 ppm.

7. The system of claim 6, wherein, the CdSe / ZnS core-shell structure quantum dot has a particle size of 2-5 nm; The CdSe / ZnS core-shell structure quantum dot surface is fixed with CRISPR-Cas12a complex and oil stain specific DNA aptamer through carboxyl-amino coupling reaction.

8. The system of claim 1, wherein, The electrochemical concentration unit adopts IrO2-Ta2O5 / Ti anode and nitrogen-doped graphene cathode.

9. A ship sewage treatment method using the ship sewage treatment system according to any one of claims 1 to 8, comprising the following steps: Detecting the concentration of pollutants in the sewage through the intelligent sensing module and transmitting the data to the intelligent control module in real time; In the plasma-acoustic chemical synergistic reactor, heavy metals are adsorbed by magnetic nanoparticles, and organic matter is degraded by active oxygen species generated by ultrasonic cavitation and plasma activation; In the synthetic microbial biomimetic reactor, biodegradation is carried out by gene editing bacteria, and the hydraulic retention time is controlled at 4±0.5 hours; Fresh water is separated from concentrated liquid by the biomimetic cell membrane intelligent filtration system, the fresh water is reused, and the concentrated liquid enters the electrochemical concentration unit to recover phosphate and lithium ions; Residual pollutants are treated by photocatalysis-microbial fuel cell and converted into electric energy, and the net energy production of the system is ≥0.4 kWh / m3; Through the digital twin platform, the parameters of each module are dynamically adjusted, so that the discharge water quality is lower than 50% of the international maritime organization limit value.

10. The method of claim 9, wherein, The recovery rate of the magnetic nanoparticles is ≥98%, and after recovery, they are regenerated by 0.1 M citric acid solution; The pH value of the sonochemical oxidation stage is maintained at 3-5 by an automatic dosing system, and the hydroxyl radical concentration is ≥1×10 -4 mol / L.

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