Composite biological deodorant and preparation method thereof

Abstract

The invention discloses a composite biological deodorant which comprises the following components in parts by weight: 1-3 parts of bacillus coagulans bacterial sludge; 1-3 parts of saccharomyces cerevisiae mud; 1-3 parts of plant lactobacillus bacterial sludge; 10-30 parts of a nutrient solution A; and 20-60 parts of a nutrient solution B. The composite biological deodorant is prepared by innovatively adopting a compound system of bacillus coagulans bacterial sludge, saccharomyces cerevisiae bacterial sludge and plant lactobacillus bacterial sludge and combining with a specific nutrient solution carrier, and bacillus coagulans provides environmental tolerance guarantee for a compound system by virtue of strong environmental resistance; metabolites secreted by the saccharomyces cerevisiae are beneficial to promoting proliferation of the plant lactobacillus, and bacteriocin generated by the plant lactobacillus selectively inhibits staphylococcus aureus, escherichia coli and other malodorous pathogenic bacteria and degrades ammonia nitrogen, hydrogen sulfide and other micromolecular malodorous substances at the same time; the three components have a synergistic effect, so that a remarkable deodorization and bacteriostasis effect is achieved, the product is free of strong peculiar smell, and the risk of infectious microbe pollution is effectively avoided.

Description

Technical Field

[0001] This invention relates to the field of microbial preparation technology, specifically to a composite biological deodorant and its preparation method. Background Technology

[0002] With increasing environmental and health requirements, biological deodorizers are becoming increasingly widely used in areas such as municipal solid waste treatment, sewage treatment, livestock farms, and residential environment improvement due to their environmental friendliness and high safety. Existing biological deodorizers are generally composed of a compound microbial fermentation broth, utilizing the synergistic effect of various strains to decompose odors and achieve a deodorizing effect. However, these biological deodorizers tend to produce a strong odor after a certain period of storage, potentially causing secondary odor pollution and increasing the risk of microbial contamination, making them particularly unsuitable for use in sensitive residential environments. Furthermore, liquid compound deodorizers generally face the problem of low survival rates of effective bacteria. For example, non-spore-forming bacteria such as *Lactobacillus plantarum* and yeast exhibit extremely rapid activity decay under normal storage conditions (the inactivation rate often exceeds 90% within 3 months at room temperature), resulting in a short shelf life and a significant decrease in deodorizing efficiency after a period of storage. Some studies have shown that solid microbial agents prepared using freeze-drying technology can extend the survival time of certain microbial species to some extent, but this process is costly and mainly applicable to highly resistant spore-forming bacteria, making it difficult to economically and effectively maintain the long-term stability of multi-strain compound systems.

[0003] Chinese patent application CN202410918462.5 discloses a method for preparing a microbial deodorant. Lactic acid bacteria, Bacillus, and yeast are cultured separately in liquid, then mixed in a specific ratio to form a composite bacterial solution. The composite bacterial solution is then mixed evenly with a carrier and dried to obtain the microbial deodorant. The method uses biochar-supported modified polyaspartic acid and β-cyclodextrin as the microbial carrier to improve the deodorizing effect. However, the carrier preparation process in this method is complex, resulting in high actual production costs. Furthermore, the carrier-based deodorant has limited contact area with the odor source, leading to slow absorption of odor molecules and significantly limiting its applicability compared to liquid deodorants.

[0004] On the other hand, current biological deodorizers rely on microbial metabolism to degrade malodorous substances. Microorganisms need a long time to adapt to the malodorous environment before they can effectively play a role, which is slow and limits their application.

[0005] Therefore, for liquid biological deodorizers, how to reduce the risk of product odor and contamination by miscellaneous bacteria, improve deodorization efficiency and long-lasting deodorization ability, and extend the shelf life of the composite microbial system are key technical problems that urgently need to be solved in this field. Summary of the Invention

[0006] To overcome the shortcomings of the existing technology, the present invention aims to provide a composite biological deodorizer that can efficiently and quickly deodorize, provide long-lasting antibacterial protection, and has high storage stability.

[0007] This invention is achieved through the following technical solution: In a first aspect, the present invention provides a composite biological deodorant, comprising the following components by weight: 1-3 parts of Bacillus coagulans bacterial sludge; 1-3 parts brewer's yeast slurry; 1-3 parts of Bacillus plantarum mycelium sludge; 10-30 portions of nutrient solution A; 20-60 portions of nutrient solution B; The Bacillus coagulans sludge is a solid substance obtained by fermenting and centrifuging Bacillus coagulans with accession number CICC 23843, followed by removing the supernatant. Its viable cell count is 5 × 10⁻⁶. 9 ~9×10 9 cfu / g; The brewer's yeast sludge is a solid substance obtained by fermenting and centrifuging brewer's yeast with accession number CICC 1450, and removing the supernatant. Its viable cell count is 1×10⁻⁶. 9 ~5×10 9 cfu / g; The *Lactobacillus plantarum* mycelium sludge is a solid substance obtained by fermenting and centrifuging *Lactobacillus plantarum* with accession number CICC 21791, followed by removal of the supernatant. Its viable cell count is 1 × 10⁻⁶. 10 ~5×10 10 cfu / g; The nutrient solution A comprises: sodium acetate 12-15 g / L, ammonium sulfate 1.5-2 g / L, sodium dihydrogen phosphate 0.9-1.1 g / L, disodium hydrogen phosphate 0.9-1.1 g / L, potassium chloride 3-5 g / L, magnesium sulfate 0.08-0.12 g / L, manganese sulfate 0.04-0.05 g / L, and trace element supplement 0.22-0.32 g / L; the pH of nutrient solution A is 4.5-5. The nutrient solution B comprises: glucose 15-20 g / L, sodium acetate 3-5 g / L, peptone 8-12 g / L, Tween 80 0.8-1.2 ml / L, sodium dihydrogen phosphate 0.9-1.1 g / L, disodium hydrogen phosphate 0.9-1.1 g / L, potassium chloride 3-5 g / L, magnesium sulfate 0.08-0.12 g / L, and manganese sulfate 0.04-0.05 g / L. The pH of nutrient solution B is 4.5-5.

[0008] This invention provides a method for preparing the *Bacillus coagulans* sludge, comprising the following steps: *Bacillus coagulans* is inoculated into LB medium at a rate of 0.5-1.1% and cultured at 28-35℃ and 160-180 rpm / min for 24-30 h to obtain seed culture A; seed culture A is inoculated into *Bacillus coagulans* fermentation medium at a rate of 2-5% and cultured at 40-45℃ and 160-180 rpm / min for 30-36 h to obtain *Bacillus coagulans* fermentation broth; the fermentation broth is centrifuged at 5000-6000 rpm / min, and the supernatant is discarded to obtain *Bacillus coagulans* sludge; the LB medium comprises 10.0 g / L peptone, 5.0 g / L yeast extract, and 10.0 g / L NaCl; the fermentation medium comprises 8.0-10.0 g / L molasses and 18.0-20.0 g / L yeast extract. g / L, NaCl 4.0-5.0 g / L, K2HPO44.0-5.0 g / L, MnSO48.0-10.0 mg / L.

[0009] This invention provides a method for preparing the *Saccharomyces cerevisiae* sludge, comprising the following steps: *Saccharomyces cerevisiae* is inoculated into YPD medium at a rate of 0.5-1% and cultured at 28-35℃ and 160-180 rpm / min for 24-30 hours to obtain seed culture B; seed culture B is inoculated into YPD medium at a rate of 1-3% and cultured at 25-30℃ and 160-180 rpm / min; when the pH decrease rate is less than 0.01, sodium acetate solution is added once to bring the sodium acetate concentration in the fermentation system to 7-10 g / L, and fermentation continues until the pH change rate is less than 0.01, yielding *Saccharomyces cerevisiae* fermentation broth; the fermentation broth is centrifuged at 3000-4000 rpm / min, and the supernatant is discarded to obtain *Saccharomyces cerevisiae* sludge; the YPD medium comprises 20.0 g / L glucose, 10.0 g / L yeast extract, and 20.0 g / L peptone.

[0010] This invention provides a method for preparing *Lactobacillus plantarum* sludge, comprising the following steps: inoculating *Lactobacillus plantarum* into MRS medium at an inoculum of 3-5%, and culturing at 28-35℃ for 40-48 hours to obtain seed culture C; inoculating seed culture C into MRS medium at an inoculum of 5-10%, and culturing at 28-35℃ for 40-48 hours to obtain *Lactobacillus plantarum* fermentation broth; centrifuging the *Lactobacillus plantarum* fermentation broth at 3000-3500 rpm / min and discarding the supernatant to obtain *Lactobacillus plantarum* sludge; the composition of the MRS medium includes 10.0 g / L peptone, 5.0 g / L beef extract, 4.0 g / L yeast extract, 2.0 g / L triammonium citrate, 20.0 g / L glucose, and Tween 80. 1 ml, sodium acetate 5.0 g / L, dipotassium hydrogen phosphate 2.0 g / L, magnesium sulfate 0.2 g / L, manganese sulfate 0.05 g / L, pH=6.4±0.2.

[0011] Furthermore, the trace element supplement in the nutrient solution A includes at least one of the following: disodium ethylenediaminetetraacetate, zinc sulfate heptahydrate, boric acid, manganese chloride tetrahydrate, cobalt chloride hexahydrate, copper sulfate pentahydrate, ammonium molybdate tetrahydrate, and ferrous sulfate heptahydrate.

[0012] Furthermore, the content of trace element supplements in the nutrient solution A is as follows: disodium ethylenediaminetetraacetate 0.05-0.075 g / L, zinc sulfate heptahydrate 0.022-0.033 g / L, boric acid 0.0114-0.171 g / L, manganese chloride tetrahydrate 0.0506-0.0759 g / L, cobalt chloride hexahydrate 0.0161-0.02415 g / L, copper sulfate pentahydrate 0.0157-0.02355 g / L, ammonium molybdate tetrahydrate 0.011-0.0165 g / L, and ferrous sulfate heptahydrate 0.0499-0.0499 g / L.

[0013] Furthermore, the nutrient solution B also includes cyclodextrin, citrus (CITRUS RETICULATA) peel extract and citric acid; the cyclodextrin content in the nutrient solution B is 0.8-1.2 g / L, the citrus (CITRUS RETICULATA) peel extract content is 3-5 g / L, and the pH of the nutrient solution is adjusted to 4.5-5 with citric acid.

[0014] The citrus (CITRUS RETICULATA) peel extract of the present invention can be obtained commercially or by self-production.

[0015] The cyclodextrin is preferably β-cyclodextrin.

[0016] Furthermore, the composite biological deodorizer includes biological deodorizer liquid A and biological deodorizer liquid B; The Bacillus coagulans sludge, Saccharomyces cerevisiae sludge, and nutrient solution A constitute biological deodorant solution A. The plant lactobacillus sludge and nutrient solution B constitute biological deodorizer solution B.

[0017] Furthermore, the biological deodorant liquid B also includes 1.5-3 wt% mannitol, 0.6-1 wt% inulin, 0.8-1.5 wt% skim milk, 0.03-0.06 wt% tea polyphenols, and 0.02-0.05 wt% sodium tripolyphosphate.

[0018] Secondly, the present invention provides a method for preparing a composite biological deodorizer, comprising the following steps: mixing the components evenly according to the ratio to prepare the composite biological deodorizer.

[0019] This invention also provides another method for preparing a composite biological deodorant, comprising the following steps: S1. Prepare biological deodorant solution A: According to the formula, Bacillus coagulans sludge, Saccharomyces cerevisiae sludge and nutrient solution A are mixed to obtain biological deodorant solution A. S2. Prepare biological deodorant solution B: According to the formula, the plant lactobacillus sludge and nutrient solution B are mixed to obtain biological deodorant solution B. Biological deodorant solution A and biological deodorant solution B are stored in different containers. When using them, biological deodorant solution A and biological deodorant solution B are mixed to obtain a composite biological deodorant.

[0020] Thirdly, the present invention provides a deodorizing spray, comprising the composite biological deodorizer described in the present invention.

[0021] The present invention has the following beneficial effects: 1. This invention innovatively employs a compound system of Bacillus coagulans, Saccharomyces cerevisiae, and Lactobacillus plantarum, combined with an odorless nutrient solution carrier, to formulate a composite biological deodorizer. Bacillus coagulans, with its strong environmental resistance, provides environmental tolerance for the composite system. The metabolites secreted by Saccharomyces cerevisiae help promote the proliferation of Lactobacillus plantarum. The bacteriocins produced by Lactobacillus plantarum (such as plant lactobacillusin) selectively inhibit malodorous pathogens such as Staphylococcus aureus and Escherichia coli, while degrading small molecule malodorous substances such as ammonia nitrogen and hydrogen sulfide. The three work synergistically to achieve a significant deodorizing and antibacterial effect. The product has no strong odor and effectively avoids the risk of contamination by miscellaneous bacteria.

[0022] 2. The composite biological deodorizer of the present invention further incorporates citric acid, cyclodextrin, and citrus peel extract to form a composite functional adjuvant, achieving a breakthrough optimization in deodorization timeliness: the effective bacteria continuously eliminate malodorous molecules such as ammonia nitrogen and hydrogen sulfide through long-term biodegradation, while the acid neutralization ability of citric acid, the molecular inclusion characteristics of cyclodextrin, and the natural adsorption activity of citrus peel extract can achieve instantaneous capture and transformation of malodorous substances, effectively making up for the delayed start-up defect of microbial deodorization.

[0023] 3. This invention innovatively designs a graded protection strategy based on the differences in the biological characteristics of bacterial strains. By using Bacillus coagulans and Saccharomyces cerevisiae bacterial sludge, most of the Bacillus coagulans in the Bacillus coagulans sludge transforms into spores during fermentation, and most of the Saccharomyces cerevisiae in the Saccharomyces cerevisiae sludge transforms into spores during fermentation. Using an oligonutrient solution to prepare biological deodorant solution A, the survival period of Bacillus coagulans and Saccharomyces cerevisiae can be effectively extended. Meanwhile, Bacterium plantarum bacterial sludge is separately prepared using a specific protectant and nutrient solution system to prepare biological deodorant solution B, which can maintain the stability and consistency of Bacterium plantarum cells and effectively extend the survival period of Bacterium plantarum. In use, only biological deodorant solution A and biological deodorant solution B need to be mixed to activate Bacillus coagulans and Saccharomyces cerevisiae to achieve efficient deodorization. This ensures that the product maintains a stable bacterial population ratio and functional consistency throughout its entire life cycle, extending the product shelf life to 1-2 years. Detailed Implementation

[0024] The following embodiments are provided to better understand the present invention and are not limited to the preferred embodiments described. They do not constitute a limitation on the content and scope of protection of the present invention. Any product that is the same as or similar to the present invention, derived by any person under the guidance of the present invention or by combining the features of the present invention with other prior art, falls within the protection scope of the present invention.

[0025] For experiments not specifically described in the examples, the procedures or conditions should be followed according to the conventional experimental procedures described in the literature in this field. Reagents or instruments whose manufacturers are not specified are all commercially available conventional reagent products.

[0026] The materials used in the embodiments and comparative examples of the present invention are described below, but are not limited to these materials: Bacillus coagulans: China Industrial Microbial Culture Collection Center, accession number: CICC 23843.

[0027] Saccharomyces cerevisiae: China Industrial Microbial Culture Collection Center, accession number: CICC 1450.

[0028] Lactobacillus plantarum: China Industrial Microbial Culture Collection Center, accession number: CICC 21791.

[0029] LB medium: peptone 10.0 g / L, yeast extract 5.0 g / L, NaCl 10.0 g / L, pH=7±0.2, autoclaved at 121℃ for 30 min.

[0030] YPD medium: glucose 20.0 g / L, yeast extract 10.0 g / L, peptone 20.0 g / L, autoclaved at 115℃ for 15 min.

[0031] MRS medium: peptone 10.0 g / L, beef extract 5.0 g / L, yeast extract 4.0 g / L, triammonium citrate 2.0 g / L, glucose 20.0 g / L, Tween 80 1 ml, sodium acetate 5.0 g / L, dipotassium hydrogen phosphate 2.0 g / L, magnesium sulfate 0.2 g / L, manganese sulfate 0.05 g / L, pH=6.4±0.2, sterilized at 121℃ for 30 min.

[0032] Fermentation medium for Bacillus coagulans: molasses 10.0 g / L, yeast extract powder 20.0 g / L, NaCl 5.0 g / L, K2HPO4 5.0 g / L, MnSO4 10.0 mg / L, pH=6.0-6.5, autoclaved at 115℃ for 15 min.

[0033] Bacillus coagulans mycelial sludge: This was prepared in-house using the following method: Bacillus coagulans was inoculated into LB medium at a 1% inoculum and cultured at 30℃ and 180 rpm / min for 24 h to obtain seed culture A. Seed culture A was then inoculated into Bacillus coagulans fermentation medium at a 2% inoculum and cultured at 45℃ and 180 rpm / min for 36 h to obtain Bacillus coagulans fermentation broth. The fermentation broth was centrifuged at 5000 rpm / min, and the supernatant was discarded to obtain Bacillus coagulans mycelial sludge. Its viable cell count was 5 × 10⁻⁶. 9 ~9×10 9 cfu / g.

[0034] Saccharomyces cerevisiae sludge: Prepared in-house using the following method: Saccharomyces cerevisiae was inoculated into YPD medium at a 1% inoculum and cultured at 30℃ and 180 rpm / min for 24 hours to obtain seed culture B. Seed culture B was then inoculated into YPD medium at a 1% inoculum and cultured at 25℃ and 180 rpm / min. When the pH decrease rate was less than 0.01, sodium acetate solution was added once to bring the sodium acetate concentration in the fermentation system to 8 g / L. Fermentation continued until the pH change rate was less than 0.01, yielding the Saccharomyces cerevisiae fermentation broth. The fermentation broth was centrifuged at 3000 rpm / min, and the supernatant was discarded to obtain the Saccharomyces cerevisiae sludge. Its viable cell count was 1 × 10⁻⁶.9 ~5×10 9 cfu / g.

[0035] *Lactobacillus plantarum* mycelial sludge: This was prepared in-house using the following method: *Lactobacillus plantarum* was inoculated into MRS medium at a 5% inoculum and cultured at 30℃ for 40 hours to obtain seed culture C; seed culture C was then inoculated into MRS medium at a 10% inoculum and cultured at 30℃ for 40 hours to obtain *Lactobacillus plantarum* fermentation broth; the fermentation broth was centrifuged at 3500 rpm / min, and the supernatant was discarded to obtain *Lactobacillus plantarum* mycelial sludge; its viable cell count was 1 × 10⁻⁶. 10 ~5×10 10 cfu / g.

[0036] Citrus peel extract solution: The content of citrus (CITRUS RETICULATA) peel extract is 10%, Guangzhou Zhongguang Biotechnology Co., Ltd.

[0037] Cyclodextrin: β-cyclodextrin, commercially available.

[0038] Examples 1-3 and Comparative Examples 1-6 A composite biological deodorant, comprising Bacillus coagulans sludge, Saccharomyces cerevisiae sludge, Bacillus plantarum sludge, nutrient solution A and nutrient solution B; Nutrient solution A formula: Sodium acetate 15g / L, ammonium sulfate 2g / L, sodium dihydrogen phosphate 1g / L, disodium hydrogen phosphate 1g / L, potassium chloride 5g / L, magnesium sulfate 0.1g / L, manganese sulfate 0.05g / L, disodium ethylenediaminetetraacetate 0.05g / L, zinc sulfate heptahydrate 0.022g / L, boric acid 0.0114g / L, manganese chloride tetrahydrate 0.0506g / L, cobalt chloride hexahydrate 0.0161g / L, copper sulfate pentahydrate 0.0157g / L, ammonium molybdate tetrahydrate 0.011g / L, ferrous sulfate heptahydrate 0.0499g / L; Nutrient solution B formula: glucose 20g / L, sodium acetate 5g / L, peptone 10g / L, Tween 80 1ml / L, sodium dihydrogen phosphate 1g / L, disodium hydrogen phosphate 1g / L, potassium chloride 5g / L, magnesium sulfate 0.1g / L, manganese sulfate 0.05g / L, nutrient solution pH=4.5-5.

[0039] According to the proportions in Table 1, Bacillus coagulans sludge, Saccharomyces cerevisiae sludge, Bacillus plantarum sludge, nutrient solution A, and nutrient solution B were mixed evenly to prepare the composite biological deodorizers of Examples 1-3 and Comparative Examples 1-6. Their deodorizing and antibacterial effects were tested respectively.

[0040] Table 1: Compound formulations of the composite biological deodorizers in Examples 1-3 and Comparative Examples 1-6 (by weight parts)

[0041] 1. Ammonia removal rate η(NH3) and sulfur removal rate η(H2S) test: 10 ml of water (blank control group), the biological deodorizers of Examples 1-3, and Comparative Examples 1-6 were inoculated into 2 L sealed containers containing 100 g of fresh chicken manure. Each container contained two small beakers: one containing 30 ml of 1 mol / L dilute sulfuric acid solution, and the other containing 30 ml of 1 mol / L alkaline zinc ammonium complex solution. The containers were placed in a 30°C incubator for 7 days. The ammonia nitrogen content in the dilute sulfuric acid absorption solution was determined spectrophotometrically using Nessler's reagent; the hydrogen sulfide content in the alkaline zinc ammonium complex solution was determined spectrophotometrically using methylene blue. The ammonia removal rate η(NH3) and sulfur removal rate η(H2S) of each group of biological deodorizers were then calculated using the following two formulas: η(NH3)=(CNH3 empty-CNH3 bacteria) / CNH3 empty*100% In the formula: CNH3_blank represents the content of histamine in the blank, and CNH3_bacteria represents the content of histamine in each deodorant, in mg / L.

[0042] η(H2S)=(CH2S empty-CH2S bacteria) / CH2S empty*100% In the formula: CH2S_blank represents the hydrogen sulfide content of the blank group, and CH2S_bacteria represents the hydrogen sulfide content of each deodorant group, in mg / L.

[0043] 2. Antibacterial activity test: Common odor-producing pathogens Staphylococcus aureus and Escherichia coli were inoculated onto LB plates using the streak plate method. After incubation in a 30°C constant temperature culture incubator for 30 hours, samples were taken and inoculated into LB medium. After incubation at 30°C and 160 rpm for 30 hours, Staphylococcus aureus and Escherichia coli bacterial suspensions were obtained, respectively.

[0044] Staphylococcus aureus and Escherichia coli cultures were diluted to 10⁶ CFU / mL with LB solid medium cooled to 40°C and quickly poured into plates. After the plates solidified, Staphylococcus aureus plates and Escherichia coli plates were obtained, and 10 evenly distributed marks were made on the petri dishes.

[0045] Antimicrobial activity test of the tested biological deodorant against Staphylococcus aureus: Sterile Oxford cups were placed on the surface of Staphylococcus aureus agar plates according to the markings, and 15 μL of water, biological deodorant from Examples 1-3, and Comparative 1-6 were added to each. After drying, the Oxford cups were removed, and the plates were placed in a 30°C incubator for 24 h. The diameter of the inhibition zone was observed and measured.

[0046] Antibacterial activity test of the tested biological deodorant against Escherichia coli: Sterile Oxford cups were placed on the surface of E. coli plates according to the markings, and 15 μL of water, biological deodorants of Examples 1-3, and Comparisons 1-6 were added to the plates respectively. After drying, the Oxford cups were removed, and the plates were placed in a 30°C incubator for 24 h. The diameter of the inhibition zone was observed and measured.

[0047] Table 2: Deodorizing and antibacterial effects of the composite biological deodorizers in Examples 1-3 and Comparative Examples 1-6

[0048] A comparison of Examples 1-3 and Comparative Examples 1-6 shows that the composite biological deodorizer of the present invention, using a mixture of Bacillus coagulans, Saccharomyces cerevisiae, and Lactobacillus plantarum, can effectively remove ammonia nitrogen and hydrogen sulfide, and exhibits excellent antibacterial effects against common odor-producing pathogens such as Staphylococcus aureus and Escherichia coli. Comparative Examples 1-6, using only one or two of these bacterial sludge samples, showed poor removal effects on ammonia nitrogen and hydrogen sulfide, and poor antibacterial effects against Staphylococcus aureus and Escherichia coli.

[0049] Example 4 and Comparative Examples 7-13 A composite biological deodorant, comprising Bacillus coagulans sludge, Saccharomyces cerevisiae sludge, Bacillus plantarum sludge, nutrient solution A and nutrient solution B; Nutrient solution A formula: Sodium acetate 15g / L, ammonium sulfate 2g / L, sodium dihydrogen phosphate 1g / L, disodium hydrogen phosphate 1g / L, potassium chloride 5g / L, magnesium sulfate 0.1g / L, manganese sulfate 0.05g / L, disodium ethylenediaminetetraacetate 0.05g / L, zinc sulfate heptahydrate 0.022g / L, boric acid 0.0114g / L, manganese chloride tetrahydrate 0.0506g / L, cobalt chloride hexahydrate 0.0161g / L, copper sulfate pentahydrate 0.0157g / L, ammonium molybdate tetrahydrate 0.011g / L, ferrous sulfate heptahydrate 0.0499g / L.

[0050] Nutrient solution B formula I: glucose 20g, sodium acetate 5g / L, peptone 10g / L, Tween 80 1ml / L, sodium dihydrogen phosphate 1g / L, disodium hydrogen phosphate 1g / L, potassium chloride 5g / L, magnesium sulfate 0.1g / L, manganese sulfate 0.05g / L, cyclodextrin 1g / L, citrus peel extract solution 32ml / L. Adjust the pH of the nutrient solution to 4.5-5 with citric acid.

[0051] Nutrient solution B formula II: glucose 20g, sodium acetate 5g / L, peptone 10g / L, Tween 80 1ml / L, sodium dihydrogen phosphate 1g / L, disodium hydrogen phosphate 1g / L, potassium chloride 5g / L, magnesium sulfate 0.1g / L, manganese sulfate 0.05g / L, cyclodextrin 2g / L, citrus peel extract solution 40ml / L. Adjust the pH of the nutrient solution to 4.5-5 with dilute hydrochloric acid.

[0052] Nutrient solution B formula III: 20g glucose, 5g / L sodium acetate, 10g / L peptone, 1ml / L Tween 80, 1g / L sodium dihydrogen phosphate, 1g / L disodium hydrogen phosphate, 5g / L potassium chloride, 0.1g / L magnesium sulfate, 0.05g / L manganese sulfate, 40ml / L citrus peel extract solution. Adjust the pH of the nutrient solution to 4.5-5 with citric acid.

[0053] Nutrient solution B formula IV: glucose 20g, sodium acetate 5g / L, peptone 10g / L, Tween 80 1ml / L, sodium dihydrogen phosphate 1g / L, disodium hydrogen phosphate 1g / L, potassium chloride 5g / L, magnesium sulfate 0.1g / L, manganese sulfate 0.05g / L, cyclodextrin 2g / L. Adjust the pH of the nutrient solution to 4.5-5 with citric acid.

[0054] Nutrient solution B formula V: glucose 20g, sodium acetate 5g / L, peptone 10g / L, Tween 80 1ml / L, sodium dihydrogen phosphate 1g / L, disodium hydrogen phosphate 1g / L, potassium chloride 5g / L, magnesium sulfate 0.1g / L, manganese sulfate 0.05g / L. Adjust the pH of the nutrient solution to 3.5-4 with citric acid.

[0055] Nutrient solution B formula VI: glucose 20g, sodium acetate 5g / L, peptone 10g / L, Tween 80 1ml / L, sodium dihydrogen phosphate 1g / L, disodium hydrogen phosphate 1g / L, potassium chloride 5g / L, magnesium sulfate 0.1g / L, manganese sulfate 0.05g / L, cyclodextrin 3g / L. Adjust the pH of the nutrient solution to 4.5-5 with dilute hydrochloric acid.

[0056] Nutrient solution B formula VII: 20g glucose, 5g / L sodium acetate, 10g / L peptone, 1ml / L Tween 80, 1g / L sodium dihydrogen phosphate, 1g / L disodium hydrogen phosphate, 5g / L potassium chloride, 0.1g / L magnesium sulfate, 0.05g / L manganese sulfate, 50ml / L citrus peel extract solution. Adjust the pH of the nutrient solution to 4.5-5 with dilute hydrochloric acid.

[0057] Nutrient solution B formula VIII: glucose 20g, sodium acetate 5g / L, peptone 10g / L, Tween 80 1ml / L, sodium dihydrogen phosphate 1g / L, disodium hydrogen phosphate 1g / L, potassium chloride 5g / L, magnesium sulfate 0.1g / L, manganese sulfate 0.05g / L, cyclodextrin 3g / L, citrus peel extract solution 50ml / L. Adjust the pH of the nutrient solution to 3.5 with citric acid.

[0058] According to the proportions in Table 3, Bacillus coagulans sludge, Saccharomyces cerevisiae sludge, Bacillus plantarum sludge, and nutrient solution were mixed evenly to prepare the composite biological deodorizers of Example 4 and Comparative Examples 7-13. Their deodorizing effects were then tested.

[0059] Table 3: Compound formulations of the composite biological deodorizers in Examples 4 and Comparative Examples 7-13 (by weight)

[0060] Odor removal effect test: Take 50mL of kitchen waste leachate from 9 groups and add it to 2L containers. Take the compound biological deodorizer of Example 4 and Comparative Examples 7-13, dilute it 30 times and put it into a spray bottle. Spray an equal amount of diluted deodorizer into 8 groups of containers. Spray an equal amount of water into the remaining group of containers as a blank control group. After spraying, the odor value in the containers is measured with an odor meter at 1min, 5min, 15min, 30min, 1h and 24h.

[0061] Table 4: Odor values ​​of the composite biological deodorizers of Example 4 and Comparative Examples 7-13

[0062] As can be seen from the comparison between Example 4 and Comparative Examples 7-13, the composite biological deodorizer of the present invention further incorporates citric acid, cyclodextrin and citrus (CITRUS RETICULATA) peel extract to form a composite functional adjuvant, which effectively makes up for the defect of delayed start-up of microbial deodorization. It can have a significant deodorizing effect within 1 minute after spraying and can deodorize for a long time.

[0063] Example 5 A composite biological deodorant, comprising Bacillus coagulans sludge, Saccharomyces cerevisiae sludge, Bacillus plantarum sludge, nutrient solution A and nutrient solution B; Nutrient solution A formula: Sodium acetate 15g / L, ammonium sulfate 2g / L, sodium dihydrogen phosphate 1g / L, disodium hydrogen phosphate 1g / L, potassium chloride 5g / L, magnesium sulfate 0.1g / L, manganese sulfate 0.05g / L, disodium ethylenediaminetetraacetate 0.05g / L, zinc sulfate heptahydrate 0.022g / L, boric acid 0.0114g / L, manganese chloride tetrahydrate 0.0506g / L, cobalt chloride hexahydrate 0.0161g / L, copper sulfate pentahydrate 0.0157g / L, ammonium molybdate tetrahydrate 0.011g / L, ferrous sulfate heptahydrate 0.0499g / L.

[0064] Nutrient solution B formula: glucose 20g / L, sodium acetate 5g / L, peptone 10g / L, Tween 80 1ml / L, sodium dihydrogen phosphate 1g / L, disodium hydrogen phosphate 1g / L, potassium chloride 5g / L, magnesium sulfate 0.1g / L, manganese sulfate 0.05g / L, nutrient solution pH=4.5-5.

[0065] By weight, 1 part of Bacillus coagulans sludge, 1 part of Saccharomyces cerevisiae sludge, 1 part of Bacillus plantarum sludge, 10 parts of nutrient solution A and 20 parts of nutrient solution B are mixed evenly to prepare the composite biological deodorizer of Example 5.

[0066] Example 6 A composite biological deodorant, comprising Bacillus coagulans sludge, Saccharomyces cerevisiae sludge, Bacillus plantarum sludge, nutrient solution A and nutrient solution B; Nutrient solution A formula: Sodium acetate 15g / L, ammonium sulfate 2g / L, sodium dihydrogen phosphate 1g / L, disodium hydrogen phosphate 1g / L, potassium chloride 5g / L, magnesium sulfate 0.1g / L, manganese sulfate 0.05g / L, disodium ethylenediaminetetraacetate 0.05g / L, zinc sulfate heptahydrate 0.022g / L, boric acid 0.0114g / L, manganese chloride tetrahydrate 0.0506g / L, cobalt chloride hexahydrate 0.0161g / L, copper sulfate pentahydrate 0.0157g / L, ammonium molybdate tetrahydrate 0.011g / L, ferrous sulfate heptahydrate 0.0499g / L, pH=4.5-5; Nutrient solution B formula: glucose 20g, sodium acetate 5g / L, peptone 10g / L, Tween 80 1ml / L, sodium dihydrogen phosphate 1g / L, disodium hydrogen phosphate 1g / L, potassium chloride 5g / L, magnesium sulfate 0.1g / L, manganese sulfate 0.05g / L, cyclodextrin 1g / L, citrus peel extract solution 32ml / L, adjust the pH of the nutrient solution to 4.5-5 with citric acid; Protectants: mannitol, inulin, defatting agents, tea polyphenols, sodium tripolyphosphate; The composite biological deodorizer of Example 6 was prepared by mixing 1 part Bacillus coagulans sludge, 1 part Saccharomyces cerevisiae sludge, 1 part Bacillus plantarum sludge, 10 parts nutrient solution A, and 20 parts nutrient solution B, and then adding a protective agent (final concentration of 2 wt% mannitol, 0.6 wt% inulin, 1 wt% skim milk, 0.05 wt% tea polyphenols, and 0.05 wt% sodium tripolyphosphate).

[0067] Comparative Example 14 A compound biological deodorant, comprising Bacillus coagulans fermentation broth, Saccharomyces cerevisiae fermentation broth, and Lactobacillus plantarum fermentation broth; Preparation of Bacillus coagulans fermentation broth: Bacillus coagulans was inoculated into LB medium at a 1% inoculum and cultured at 30℃ and 180 rpm / min for 24 h to obtain seed liquid A; seed liquid A was inoculated into Bacillus coagulans fermentation medium at a 2% inoculum and cultured at 45℃ and 180 rpm / min for 36 h to obtain Bacillus coagulans fermentation broth. Preparation of Saccharomyces cerevisiae fermentation broth: Saccharomyces cerevisiae was inoculated into YPD medium at a 1% inoculum and cultured at 30℃ and 180 rpm / min for 24 h to obtain seed culture B; seed culture B was inoculated into YPD medium at a 1% inoculum and cultured at 30℃ and 180 rpm / min. When the rate of pH decrease was less than 0.01, sodium acetate solution was added once to bring the sodium acetate concentration in the fermentation system to 8 g / L. Fermentation continued until the rate of pH change was less than 0.01 to obtain Saccharomyces cerevisiae fermentation broth.

[0068] Preparation of *Lactobacillus plantarum* fermentation broth: *Lactobacillus plantarum* was inoculated into MRS medium at a 5% inoculum and cultured at 30℃ for 40 h to obtain seed culture C; seed culture C was inoculated into MRS medium at a 10% inoculum and cultured at 30℃ for 40 h to obtain *Lactobacillus plantarum* fermentation broth.

[0069] By weight, 1 part of Bacillus coagulans fermentation broth, 1 part of Saccharomyces cerevisiae fermentation broth, and 1 part of Bacillus plantarum fermentation broth were mixed evenly to obtain the compound biological deodorant of Comparative Example 14.

[0070] After opening, Examples 5-6 and Comparative Example 14 were placed in a cool, dark place, and the gas expansion, bacterial contamination, and odor value changes of the compound biological deodorant were observed every quarter.

[0071] Table 5: Changes in the composite bio-deodorant of Examples 5-6 and Comparative Example 14 during storage

[0072] As can be seen from the comparison of Examples 5-6 and Comparative Example 14, the composite biological deodorizer of the present invention, which uses Bacillus coagulans sludge, Saccharomyces cerevisiae sludge, Bacillus plantarum sludge and a specific nutrient solution system, can effectively improve the storage stability of the product, effectively avoid the risk of contamination by miscellaneous bacteria, and will not produce a strong odor during long-term storage.

[0073] Example 7 A composite biological deodorizer includes biological deodorizer liquid A and biological deodorizer liquid B; biological deodorizer liquid A consists of Bacillus coagulans sludge, Saccharomyces cerevisiae sludge and nutrient solution A; biological deodorizer liquid B consists of Bacillus plantarum sludge, nutrient solution B and protective agents (mannitol, inulin, skim milk, tea polyphenols, sodium tripolyphosphate).

[0074] Nutrient solution A formula: Sodium acetate 15g / L, ammonium sulfate 2g / L, sodium dihydrogen phosphate 1g / L, disodium hydrogen phosphate 1g / L, potassium chloride 5g / L, magnesium sulfate 0.1g / L, manganese sulfate 0.05g / L, disodium ethylenediaminetetraacetate 0.05g / L, zinc sulfate heptahydrate 0.022g / L, boric acid 0.0114g / L, manganese chloride tetrahydrate 0.0506g / L, cobalt chloride hexahydrate 0.0161g / L, copper sulfate pentahydrate 0.0157g / L, ammonium molybdate tetrahydrate 0.011g / L, ferrous sulfate heptahydrate 0.0499g / L; Nutrient solution B formula: glucose 20g, sodium acetate 5g / L, peptone 10g / L, Tween 80 1ml / L, sodium dihydrogen phosphate 1g / L, disodium hydrogen phosphate 1g / L, potassium chloride 5g / L, magnesium sulfate 0.1g / L, manganese sulfate 0.05g / L, cyclodextrin 1g / L, citrus peel extract solution 32ml / L, adjust the pH of the nutrient solution to 4.5-5 with citric acid; Preparation of biological deodorant solution A: Take 1 part of Bacillus coagulans sludge, 1 part of Saccharomyces cerevisiae sludge, and 10 parts of nutrient solution A and mix them to obtain biological deodorant solution A; Preparation of biological deodorant solution B: Mix 1 part of Bacillus plantarum sludge and 20 parts of nutrient solution B, and add a protective agent (final concentration of mannitol 2wt%, inulin 0.6wt%, skim milk 1wt%, tea polyphenols 0.05wt%, sodium tripolyphosphate 0.05wt%) to obtain biological deodorant solution B.

[0075] Storage and use of the compound biological deodorizer: Store biological deodorizer A and biological deodorizer B in separate containers and keep them in an environment protected from light and with a temperature below 25°C. When using this deodorizer, pour biological deodorizer A into biological deodorizer B, mix well, and let it stand for 5-6 hours before use.

[0076] Comparative Example 15 The only difference between Comparative Example 15 and Example 7 is the preparation of Bacillus coagulans sludge: Bacillus coagulans was inoculated into LB medium at a 1% inoculum and cultured at 30°C and 160 rpm / min for 24 h to obtain seed liquid A; seed liquid A was inoculated into LB medium at a 2% inoculum and cultured at 30°C and 180 rpm / min for 36 h to obtain Bacillus coagulans fermentation broth, which was centrifuged at 5000 rpm / min and the supernatant was discarded to obtain Bacillus coagulans sludge.

[0077] Comparative Example 16 The only difference between Comparative Example 16 and Example 7 is the storage method of the composite biological deodorizer: Comparative Example 16 directly mixes biological deodorizer A liquid and biological deodorizer B liquid and stores them in an environment protected from light and with a temperature below 25°C; the rest is the same.

[0078] Comparative Example 17 The only difference between Comparative Example 17 and Example 7 is the preparation of the Saccharomyces cerevisiae sludge: In Comparative Example 17, Saccharomyces cerevisiae was inoculated into YPD medium at a 1% inoculum and cultured at 30°C and 180 rpm / min for 24 h to obtain seed culture B; Seed culture B was inoculated into YPD medium at a 2% inoculum and cultured at 25°C and 180 rpm / min. When the rate of pH decrease was less than 0.01, glucose solution was added once to bring the glucose concentration in the fermentation system to 8 g / L. Fermentation continued until the rate of pH decrease was less than 0.01 to obtain Saccharomyces cerevisiae fermentation broth. The sludge was obtained by centrifugation at 3500 rpm / min.

[0079] Samples of the biological deodorizing liquid A from Examples 7, 15, and 17, and the biological deodorizing liquid from Comparative Example 16, were taken every month after opening and plated on LB and PDA plates to determine the viable counts of Bacillus coagulans and Saccharomyces cerevisiae. Samples of the biological deodorizing liquid B from Examples 7, 15, and 17, and the biological deodorizing liquid from Comparative Example 16, were taken every month after opening and plated on MRS plates to determine the viable counts of Lactobacillus plantarum. The shelf life of the bacteria was recorded at the point when the viable counts of Bacillus coagulans, Saccharomyces cerevisiae, and Lactobacillus plantarum dropped to 10%. The shortest shelf life of Bacillus coagulans, Saccharomyces cerevisiae, and Lactobacillus plantarum in the deodorant was selected as the total shelf life of the deodorant (i.e., ensuring that the viable counts of each strain in the compound biological deodorant are all above 10%).

[0080] Table 6: Shelf life of the deodorants in Examples 7 and Comparative Examples 15-17

[0081] Unopened portions of the compound biological deodorizer from Example 7, stored for 1 month, 6 months, 12 months, 18 months, 24 months, and 25 months respectively, were mixed and activated. The ammonia removal rate, sulfur removal rate, antibacterial properties, and deodorization effect were tested. An equal volume of water was used as a blank control group. (The experimental procedures for the first three indicators were the same as those in Examples 1-3 and Comparative Examples 1-6; the experimental procedures for the last indicator were the same as those in Examples 4 and Comparative Examples 7-13.) Table 7: Deodorizing and antibacterial effects of the compound biological deodorizer in Example 7 at different storage times

[0082] Table 8: Odor values ​​of the compound biological deodorant in Example 7 at different storage times

[0083] As can be seen from the comparison of Example 7 and Comparative Examples 15-17, the composite biological deodorizer of the present invention uses Bacillus coagulans sludge and Saccharomyces cerevisiae sludge to prepare biological deodorizer A solution using an oligonutrient solution, and uses Bacillus plantarum sludge to prepare biological deodorizer B solution using a specific protectant and nutrient solution system. Before use, biological deodorizer A solution and biological deodorizer B solution are stored separately, and the shelf life of the product can be extended to 1-2 years.

[0084] Example 8 Preparation of biological deodorant solution B: By weight, take 1 part of Bacillus plantarum sludge and 20 parts of nutrient solution B, mix them together, and add a protective agent (final concentration of mannitol 2wt%, inulin 0.6wt%, skim milk 1wt%, tea polyphenols 0.05wt%, sodium tripolyphosphate 0.05wt%) to obtain biological deodorant solution B.

[0085] Nutrient solution B formula: 20g glucose, 5g / L sodium acetate, 10g / L peptone, 1ml / L Tween 80, 1g / L sodium dihydrogen phosphate, 1g / L disodium hydrogen phosphate, 5g / L potassium chloride, 0.1g / L magnesium sulfate, 0.05g / L manganese sulfate, 1g / L cyclodextrin, 32ml / L citrus peel extract solution. Adjust the pH of the nutrient solution to 4.5-5 with citric acid.

[0086] Comparative Examples 18-27 The only difference between Comparative Examples 18-27 and Example 8 is the formulation of the added protective agent. The specific formulations of each group of protective agents are shown in Table 7.

[0087] Table 9: Formulations of the protective agents added in Examples 8 and Comparative Examples 18-27

[0088] The effective viable bacteria count of the bacterial solutions from Examples 8 and Comparative Examples 18-27 was measured using MRS plates every month until the effective viable bacteria count of Bacillus plantarum dropped below 10%. This time period was recorded as the shelf life of the biological deodorant.

[0089] Table 8: Shelf life of biological deodorant liquid B in Examples 8 and Comparative Examples 18-27

[0090] A comparison of Examples 8 and Comparative Examples 18-27 shows that among various protective agents, sugars and glycolipids have the best protective effect on *Lactobacillus plantarum*, likely due to their protective effect on the *Lactobacillus plantarum* biofilm. For cost considerations, sufficient glucose was added to the nutrient solution, and 2% mannitol and 0.6% inulin were added to the protective agent. The protective effect of skim milk on *Lactobacillus plantarum* is likely due to its rich protein content, which protects the proteins in the *Lactobacillus plantarum* biofilm. Tea polyphenols have an antioxidant effect, thus reducing cell damage. Sodium tripolyphosphate acts as a water-retaining agent and thickener, stabilizing the cells and solution. This invention uses a specific protective agent and nutrient solution system to formulate *Lactobacillus plantarum* bacterial sludge into a biological deodorizing agent B solution, which can effectively solve the problem of short shelf life of *Lactobacillus plantarum*.

[0091] The above embodiments are merely illustrative examples for clear explanation and are not intended to limit the implementation. Those skilled in the art will recognize that other variations or modifications can be made based on the above description. It is neither necessary nor possible to exhaustively list all possible implementations. However, obvious variations or modifications derived therefrom are still within the scope of protection of this invention.

Claims

1. A composite biological deodorant, characterized in that, By weight, it includes the following components: 1-3 parts of Bacillus coagulans bacterial sludge; 1-3 parts brewer's yeast slurry; 1-3 parts of Bacillus plantarum mycelium sludge; 10-30 portions of nutrient solution A; 20-60 portions of nutrient solution B; The Bacillus coagulans sludge is a solid substance obtained by fermenting and centrifuging Bacillus coagulans with accession number CICC 23843, followed by removing the supernatant. Its viable cell count is 5 × 10⁻⁶. 9 ~9×10 9 cfu / g; The brewer's yeast sludge is a solid substance obtained by fermenting and centrifuging brewer's yeast with accession number CICC 1450, and removing the supernatant. Its viable cell count is 1×10⁻⁶. 9 ~5×10 9 cfu / g; The *Lactobacillus plantarum* mycelium sludge is a solid substance obtained by fermenting and centrifuging *Lactobacillus plantarum* with accession number CICC 21791, followed by removal of the supernatant. Its viable cell count is 1 × 10⁻⁶. 10 ~5×10 10 cfu / g; The nutrient solution A comprises: sodium acetate 12-15 g / L, ammonium sulfate 1.5-2 g / L, sodium dihydrogen phosphate 0.9-1.1 g / L, disodium hydrogen phosphate 0.9-1.1 g / L, potassium chloride 3-5 g / L, magnesium sulfate 0.08-0.12 g / L, manganese sulfate 0.04-0.05 g / L, and trace element supplement 0.22-0.32 g / L; the pH of nutrient solution A is 4.5-5. The nutrient solution B comprises: glucose 15-20 g / L, sodium acetate 3-5 g / L, peptone 8-12 g / L, Tween 80 0.8-1.2 ml / L, sodium dihydrogen phosphate 0.9-1.1 g / L, disodium hydrogen phosphate 0.9-1.1 g / L, potassium chloride 3-5 g / L, magnesium sulfate 0.08-0.12 g / L, and manganese sulfate 0.04-0.05 g / L. The pH of nutrient solution B is 4.5-5.

2. The composite biological deodorizer according to claim 1, characterized in that, The preparation method of the Bacillus coagulans sludge includes the following steps: Bacillus coagulans is inoculated into LB medium at an inoculum of 0.5-1.1% and cultured at 28-35℃ and 160-180 rpm / min for 24-30 h to obtain seed solution A; seed solution A is inoculated into Bacillus coagulans fermentation medium at an inoculum of 2-5% and cultured at 40-45℃ and 160-180 rpm / min for 30-36 h to obtain Bacillus coagulans fermentation broth; the Bacillus coagulans fermentation broth is centrifuged at 5000-6000 rpm / min, and the supernatant is discarded to obtain Bacillus coagulans sludge; the LB medium comprises 10.0 g / L peptone, 5.0 g / L yeast extract, and 10.0 g / L NaCl; the Bacillus coagulans fermentation medium comprises 8.0-10.0 g / L molasses and 18.0-20.0 g / L yeast extract. g / L, NaCl 4.0-5.0 g / L, K2HPO4 4.0-5.0 g / L, MnSO4 8.0-10.0 mg / L; The preparation method of the brewing yeast sludge includes the following steps: Brewing yeast is inoculated into YPD medium at a rate of 0.5-1%, and cultured at 28-35℃ and 160-180 rpm / min for 24-30 hours to obtain seed culture B; seed culture B is inoculated into YPD medium at a rate of 1-3%, and cultured at 25-30℃ and 160-180 rpm / min. When the pH decrease rate is less than 0.01, sodium acetate solution is added once to bring the sodium acetate concentration in the fermentation system to 7-10 g / L, and fermentation continues until the pH change rate is less than 0.01, yielding brewing yeast fermentation broth; the brewing yeast fermentation broth is centrifuged at 3000-4000 rpm / min, and the supernatant is discarded to obtain brewing yeast sludge; the YPD medium comprises 20.0 g / L glucose, 10.0 g / L yeast extract, and 20.0 g / L peptone. The preparation method of the *Lactobacillus plantarum* sludge includes the following steps: *Lactobacillus plantarum* is inoculated into MRS medium at a 3-5% inoculum and cultured at 28-35℃ for 40-48 hours to obtain seed culture C; seed culture C is inoculated into MRS medium at a 5-10% inoculum and cultured at 28-35℃ for 40-48 hours to obtain *Lactobacillus plantarum* fermentation broth; the fermentation broth is centrifuged at 3000-3500 rpm / min, and the supernatant is discarded to obtain *Lactobacillus plantarum* sludge; the MRS medium comprises 10.0 g / L peptone, 5.0 g / L beef extract, 4.0 g / L yeast extract, 2.0 g / L triammonium citrate, 20.0 g / L glucose, and Tween 80... 1 ml, sodium acetate 5.0 g / L, dipotassium hydrogen phosphate 2.0 g / L, magnesium sulfate 0.2 g / L, manganese sulfate 0.05 g / L, pH=6.4±0.

2.

3. The composite biological deodorizer according to claim 1, characterized in that, The trace element supplement in the nutrient solution A includes at least one of the following: disodium ethylenediaminetetraacetate, zinc sulfate heptahydrate, boric acid, manganese chloride tetrahydrate, cobalt chloride hexahydrate, copper sulfate pentahydrate, ammonium molybdate tetrahydrate, and ferrous sulfate heptahydrate.

4. The composite biological deodorizer according to claim 3, characterized in that, The trace element supplements in nutrient solution A contain the following amounts: disodium ethylenediaminetetraacetate 0.05-0.075 g / L, zinc sulfate heptahydrate 0.022-0.033 g / L, boric acid 0.0114-0.171 g / L, manganese chloride tetrahydrate 0.0506-0.0759 g / L, cobalt chloride hexahydrate 0.0161-0.02415 g / L, copper sulfate pentahydrate 0.0157-0.02355 g / L, ammonium molybdate tetrahydrate 0.011-0.0165 g / L, and ferrous sulfate heptahydrate 0.0499-0.0499 g / L.

5. The composite biological deodorizer according to claim 1, characterized in that, The nutrient solution B also includes cyclodextrin, citrus (CITRUS RETICULATA) peel extract and citric acid; the cyclodextrin content in the nutrient solution B is 0.8-1.2 g / L, the citrus (CITRUS RETICULATA) peel extract content is 3-5 g / L, and the pH of the nutrient solution is adjusted to 4.5-5 with citric acid.

6. The composite biological deodorizer according to claim 1, characterized in that, Includes biological deodorant solution A and biological deodorant solution B; The Bacillus coagulans sludge, Saccharomyces cerevisiae sludge, and nutrient solution A constitute biological deodorant solution A. The plant lactobacillus sludge and nutrient solution B constitute biological deodorizer solution B.

7. The composite biological deodorizer according to claim 6, characterized in that, The biological deodorant solution B also includes 1.5-3 wt% mannitol, 0.6-1 wt% inulin, 0.8-1.5 wt% skim milk, 0.03-0.06 wt% tea polyphenols, and 0.02-0.05 wt% sodium tripolyphosphate.

8. A method for preparing the composite biological deodorizer according to any one of claims 1-5, characterized in that, The process includes the following steps: mixing the components evenly according to the specified ratio to prepare a composite biological deodorizer.

9. The method for preparing the composite biological deodorizer according to claim 6, characterized in that, Includes the following steps: S1. Prepare biological deodorant solution A: According to the formula, Bacillus coagulans sludge, Saccharomyces cerevisiae sludge and nutrient solution A are mixed to obtain biological deodorant solution A. S2. Prepare biological deodorant solution B: According to the formula, the plant lactobacillus sludge and nutrient solution B are mixed to obtain biological deodorant solution B. Biological deodorant solution A and biological deodorant solution B are stored in different containers. When using them, biological deodorant solution A and biological deodorant solution B are mixed to obtain a composite biological deodorant.

10. A deodorizing spray, characterized in that, Includes the composite biological deodorizer as described in any one of claims 1-7.

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