Composition containing anti-wrinkle lipidosome for cosmetic preparation and preparation method

Abstract

The invention relates to a composition containing anti-wrinkle lipidosome for cosmetic preparation and a preparation method of the composition, and belongs to the technical field of cosmetics. The invention discloses a composition containing anti-wrinkle lipidosome and a preparation method thereof. The composition comprises four plant extracts of purple coneflower, calendula officinalis, salvia officinalis and elderberry, and lipidosome consisting of lecithin, cholesterol, sphingomyelin and ascorbyl palmitate, and is supplemented with components such as a humectant and an emulsifier. According to the composition, through the synergistic effect of the plant extract and the lipidosome, the anti-wrinkle effect is enhanced, and the storage and illumination stability is improved; an extract structure is optimized by a compound fermentation process, and a liposome system is adapted; the components are safe and suitable for multiple skin types, the preparation process is compatible with industrial production, and the pain points that traditional anti-wrinkle products are poor in stability and insufficient in effect are solved.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, specifically relating to a composition containing anti-wrinkle liposomes for use in cosmetic preparation and a preparation method thereof. Background Technology

[0002] With the improvement of people's living standards, the demand for skin care is increasing, and anti-wrinkle has become an important research direction in the cosmetics field. Currently, most anti-wrinkle cosmetics on the market use chemically synthesized ingredients or common plant extracts, such as Centella asiatica extract and ginseng extract. However, these ingredients have some limitations: on the one hand, chemically synthesized ingredients may irritate the skin, and their long-term safety needs to be considered; on the other hand, the anti-wrinkle effect of common plant extracts is limited, and their active ingredients have poor stability, easily degrading during storage and use, affecting the product's efficacy.

[0003] Meanwhile, in existing anti-wrinkle compositions, liposomes, as a commonly used carrier system, also have shortcomings in stability and compatibility with other components. For example, the binding of liposomes to extracts is not tight enough, and phenomena such as layering and aggregation are prone to occur, resulting in poor storage stability and light stability of the composition, which in turn affects the product's efficacy and shelf life.

[0004] In addition, traditional methods for preparing plant extracts, such as solvent extraction, often fail to fully release the active ingredients in plants, and the molecular weight distribution of the extracts is uneven, making it difficult to form a stable system with liposomes, which further limits the performance improvement of the composition.

[0005] Therefore, developing a cosmetic composition with good stability, excellent anti-wrinkle effect and high safety has become a technical problem that urgently needs to be solved in this field. Summary of the Invention

[0006] The purpose of this invention is to provide a composition containing anti-wrinkle liposomes for use in cosmetic preparation and a method for its preparation. This composition exhibits excellent anti-wrinkle effects, good storage stability and photostability, and high safety, thus meeting consumers' demand for high-quality anti-wrinkle cosmetics.

[0007] The objective of this invention can be achieved through the following technical solutions:

[0008] This invention provides a composition containing anti-wrinkle liposomes for use in cosmetic preparation, the composition comprising the following components in parts by weight:

[0009] 10-30 parts of liposomes;

[0010] Purple coneflower extract 5-15 parts;

[0011] Calendula extract 5-15 parts;

[0012] Sage extract 5-15 parts;

[0013] Elderberry extract 5-15 parts;

[0014] 5-20 parts of moisturizer;

[0015] 1-10 parts emulsifier;

[0016] Preservative 0.1-1 part;

[0017] pH adjuster 0.1 part;

[0018] 20-60 parts deionized water.

[0019] As a preferred embodiment of the present invention, the liposomes are composed of the following components in parts by weight:

[0020] 50-70 parts lecithin;

[0021] Cholesterol 10-30 parts;

[0022] 10-20 parts of sphingomyelin;

[0023] Ascorbyl palmitate 1-5 parts.

[0024] As a preferred embodiment of the present invention, the purple coneflower extract, calendula extract, sage extract, and elderberry extract are all extracted by fermentation using three or more compound microbial strains after grinding the plants. The specific method is as follows:

[0025] The plant materials are ground into powder, and deionized water is added at a mass ratio of 1:5 to 1:10. After stirring evenly, a compound microbial inoculum is added, which includes:

[0026] The concentration of Lactobacillus plantarum was 1×10⁻⁶. 8 -5×10 8 CFU / g plant raw materials

[0027] The concentration of brewer's yeast is 5×10⁻⁶. 7 -3×10 8 CFU / g plant raw materials

[0028] The concentration of Bifidobacterium was 1×10⁻⁶. 8 -4×10 8 CFU / g plant raw materials

[0029] The concentration of Lactobacillus acidophilus was 5 × 10⁻⁶. 7 -2×10 8 CFU / g plant-based raw materials.

[0030] As a preferred technical solution of the present invention, the specific ratio between the strains is Lactobacillus plantarum: Saccharomyces cerevisiae: Bifidobacterium: Lactobacillus acidophilus = (2-4):(1-3):(2-3):(1-2).

[0031] As a preferred embodiment of the present invention, the fermentation extraction is carried out by fermentation for 48-72 hours at a temperature of 30-40℃ and a pH value of 5.0-6.5, followed by solid-liquid separation, collection of filtrate, and concentration under reduced pressure to obtain plant extract.

[0032] As a preferred embodiment of the present invention, the moisturizer is one or more of glycerin, propylene glycol, and 1,3-butanediol; the emulsifier is one or more of polysorbate-80, lecithin, and glyceryl stearate; the preservative is one or more of phenoxyethanol, potassium sorbate, and methylparaben; and the pH adjuster is one or more of citric acid, lactic acid, and triethanolamine.

[0033] The present invention also provides a method for preparing the above composition, comprising the following steps:

[0034] Preparation of plant extracts: Echinacea purpurea extract, calendula extract, sage extract and elderberry extract were prepared according to the above method;

[0035] Preparation of liposomes: lecithin, cholesterol, sphingomyelin and ascorbyl palmitate were mixed in proportion, an organic solvent was added, and the mixture was stirred and dissolved at 40-60℃ to form a lipid film; then an aqueous phase containing a humectant, emulsifier and some deionized water was added, and the mixture was sonicated at 50-70℃ to form a liposome suspension. The organic solvent was removed by rotary evaporation under reduced pressure to obtain liposomes.

[0036] Mixing: The prepared liposomes, plant extracts, moisturizers, emulsifiers, preservatives, pH adjusters and remaining deionized water are mixed in proportion, stirred evenly at 40-60℃, and the pH value is adjusted to 5.5-6.5 to obtain the composition.

[0037] The beneficial effects of this invention:

[0038] (1) The composition of the present invention contains four unique plant extracts (purple coneflower extract, calendula extract, sage extract and elderberry extract), which are extracted by fermentation of compound strains, so that the molecular weight of the extracts is uniform and can form a good stable system with liposomes, which significantly improves the storage stability and light stability of the composition.

[0039] (2) The addition of liposomes can not only encapsulate the active ingredients in plant extracts and improve their bioavailability, but also enhance the anti-wrinkle effect of the composition. The reasonable ratio of lecithin, cholesterol, sphingomyelin and ascorbyl palmitate gives the liposomes good stability and biocompatibility.

[0040] (3) The use of compound microbial strains can fully release the effective components in plants, such as polyphenols and flavonoids. These components have antioxidant and collagen synthesis-promoting effects, thereby enhancing the anti-wrinkle effect of the composition.

[0041] (4) The composition of the present invention does not contain common irritating ingredients, is highly safe, and is suitable for use on all skin types.

[0042] (5) The preparation method is simple, highly operable, and suitable for industrial production. Detailed Implementation

[0043] To further illustrate the technical means and effects of the present invention in achieving its intended purpose, the following detailed description of the specific implementation methods, structures, features, and effects of the present invention, in conjunction with embodiments, is provided below.

[0044] Preparation Example 1: Preparation of Echinacea purpurea extract

[0045] Grind the purple coneflower raw material into powder. Weigh 100g of the powder and add it to 800g of deionized water at a mass ratio of 1:8. Stir well and then add the compound microbial inoculum. The compound microbial inoculum includes:

[0046] Lactobacillus plantarum, concentration 3×10 8 CFU / g plant material, dosage: 3×10 10 CFU;

[0047] brewing yeast, concentration 2×10 8 CFU / g plant material, dosage is 2×10 10 CFU;

[0048] Bifidobacterium, concentration 3×10 8 CFU / g plant material, dosage: 3×10 10 CFU;

[0049] Lactobacillus acidophilus, at a concentration of 1.5 × 10⁻⁶. 8 CFU / g plant material, dosage is 1.5×10 10 CFU.

[0050] The ratio of the bacterial strains is Lactobacillus plantarum: Saccharomyces cerevisiae: Bifidobacterium: Lactobacillus acidophilus = 3:2:3:1.5.

[0051] Fermentation was carried out at 35℃ and pH 5.5 for 60 hours, followed by solid-liquid separation. The filtrate was collected and concentrated under reduced pressure to obtain Echinacea extract.

[0052] Preparation Example 2: Preparation of Calendula extract

[0053] Grind the calendula raw material into powder. Weigh 100g of the powder and add 600g of deionized water at a mass ratio of 1:6. Stir well, then add the compound microbial inoculum. The compound microbial inoculum includes:

[0054] Lactobacillus plantarum, concentration 4×10 8 CFU / g plant material, dosage: 4×10 10 CFU;

[0055] Saccharomyces cerevisiae, concentration 1×10 8 CFU / g plant material, dosage 1×10 10 CFU;

[0056] Bifidobacterium, concentration 2×10 8 CFU / g plant material, dosage is 2×10 10 CFU;

[0057] Lactobacillus acidophilus, concentration 2×10 8 CFU / g plant material, dosage is 2×10 10 CFU.

[0058] The ratio of the bacterial strains is Lactobacillus plantarum: Saccharomyces cerevisiae: Bifidobacterium: Lactobacillus acidophilus = 4:1:2:2.

[0059] Fermentation was carried out at 38℃ and pH 6.0 for 72 hours, followed by solid-liquid separation. The filtrate was collected and concentrated under reduced pressure to obtain calendula extract.

[0060] Preparation 3: Preparation of Sage Extract

[0061] Grind the sage raw material into powder, weigh 100g of powder, add 1000g of deionized water at a mass ratio of 1:10, stir well, and then add the compound microbial inoculum. The compound microbial inoculum includes:

[0062] Lactobacillus plantarum, concentration 2×10 8 CFU / g plant material, dosage is 2×10 10 CFU;

[0063] brewing yeast, concentration 3×10 8 CFU / g plant material, dosage: 3×10 10 CFU;

[0064] Bifidobacteria, concentration 2.5 × 10⁻⁶ 8 CFU / g plant material, dosage is 2.5×10 10 CFU;

[0065] Lactobacillus acidophilus, concentration 1×10 8 CFU / g plant material, dosage 1×10 10 CFU.

[0066] The ratio of the bacterial strains is Lactobacillus plantarum: Saccharomyces cerevisiae: Bifidobacterium: Lactobacillus acidophilus = 2:3:2.5:1.

[0067] Fermentation was carried out at 32℃ and pH 5.0 for 48 hours, followed by solid-liquid separation. The filtrate was collected and concentrated under reduced pressure to obtain sage extract.

[0068] Preparation Example 4: Preparation of Elderberry Extract

[0069] Grind elderberry raw materials into powder, weigh 100g of powder, add 500g of deionized water at a mass ratio of 1:5, stir well, and then add the compound microbial inoculum. The compound microbial inoculum includes:

[0070] Lactobacillus plantarum, concentration 5×10 8 CFU / g plant material, dosage: 5×10 10 CFU;

[0071] Saccharomyces cerevisiae, concentration 2.5 × 10⁻⁶ 8 CFU / g plant material, dosage is 2.5×10 10 CFU;

[0072] Bifidobacteria, concentration 3.5 × 10⁻⁶ 8 CFU / g plant material, dosage is 3.5×10 10 CFU;

[0073] Lactobacillus acidophilus, concentration 1.8 × 10⁻⁶ 8 CFU / g plant material, dosage is 1.8×10 10 CFU.

[0074] The ratio of the bacterial strains is Lactobacillus plantarum: Saccharomyces cerevisiae: Bifidobacterium: Lactobacillus acidophilus = 5:2.5:3.5:1.8.

[0075] The elderberry extract was obtained by fermentation at 40°C and pH 6.5 for 65 hours, followed by solid-liquid separation, collection of the filtrate, and concentration under reduced pressure.

[0076] Preparation Example 5: Preparation of Liposomes

[0077] Weigh out 60 parts lecithin, 20 parts cholesterol, 15 parts sphingomyelin, and 5 parts ascorbyl palmitate, mix them, and then add a chloroform and methanol mixture (2:1 volume ratio). Stir at 50°C to dissolve and form a lipid membrane. Then add an aqueous phase containing 10 parts glycerol, 5 parts polysorbate-805, and 100 parts deionized water. Sonicate at 60°C, 300W, for 20 minutes to form a liposome suspension. Remove the organic solvent by rotary evaporation under reduced pressure to obtain liposomes.

[0078] Example 1

[0079] A composition containing anti-wrinkle liposomes for use in cosmetic preparation, comprising the following components in parts by weight:

[0080] 20 portions of liposomes;

[0081] 10 parts of purple coneflower extract;

[0082] Calendula extract 10 parts;

[0083] 10 parts of sage extract;

[0084] 10 parts elderberry extract;

[0085] 15 parts glycerin;

[0086] 5 parts of polysorbate-80 (purchased from Guangzhou Yuanzheng Chemical Co., Ltd., brand: Hai Shi Hua, item number: 15014232342);

[0087] 0.5 parts of phenoxyethanol;

[0088] Citric acid 0.3 parts;

[0089] 40 parts of deionized water.

[0090] Preparation method:

[0091] Purple coneflower extract, calendula extract, sage extract and elderberry extract were prepared according to preparation examples 1-4 respectively.

[0092] Liposomes were prepared according to Preparation Example 5;

[0093] The prepared liposomes, plant extracts, glycerol, polysorbate-80, phenoxyethanol, citric acid and deionized water were mixed and stirred evenly at 50°C. The pH was adjusted to 5.8 to obtain the composition.

[0094] Example 2

[0095] A composition containing anti-wrinkle liposomes for use in cosmetic preparation, comprising the following components in parts by weight:

[0096] 15 portions of liposomes;

[0097] 8 parts of purple coneflower extract;

[0098] Calendula extract 12 parts;

[0099] 8 parts of sage extract;

[0100] 12 portions of elderberry extract;

[0101] 12 parts propylene glycol;

[0102] 8 parts lecithin;

[0103] Potassium sorbate 0.6 parts;

[0104] Lactic acid 0.4 parts;

[0105] 45 parts of deionized water.

[0106] The preparation method is similar to that in Example 1, except for the amount of each component and the types of emulsifier, humectant, preservative and pH adjuster.

[0107] Example 3

[0108] A composition containing anti-wrinkle liposomes for use in cosmetic preparation, comprising the following components in parts by weight:

[0109] 25 portions of liposomes;

[0110] 12 parts of purple coneflower extract;

[0111] Calendula extract, 8 parts;

[0112] 12 parts of sage extract;

[0113] 8 portions of elderberry extract;

[0114] 18 parts of 1,3-butanediol;

[0115] 6 parts of glyceryl stearate;

[0116] Methylparaben 0.4 parts;

[0117] 0.5 parts of triethanolamine;

[0118] 30 parts deionized water.

[0119] The preparation method is similar to that in Example 1, except for the amount of each component and the types of emulsifier, humectant, preservative and pH adjuster.

[0120] Comparative Example 1

[0121] Compared with Example 1, the purple coneflower extract was missing, while the other components and amounts were the same.

[0122] Comparative Example 2

[0123] Compared to Example 1, this example lacks calendula extract, but the other components and amounts are the same.

[0124] Comparative Example 3

[0125] Compared to Example 1, this example lacks sage extract, but the other components and amounts are the same.

[0126] Comparative Example 4

[0127] Compared to Example 1, elderberry extract was omitted, while the other components and amounts were the same.

[0128] Comparative Example 5

[0129] Compared with Example 1, the plant extract was prepared by solvent extraction (70% ethanol concentration, 60°C, 2 hours), with the other components and amounts being the same.

[0130] Comparative Example 6

[0131] Compared to Example 1, the compound strain lacks *Lactobacillus plantarum*. Other strains and their amounts are adjusted proportionally (referring to the ratio between individual components and the remaining total components; for example, the ratio of the remaining strains *Saccharomyces cerevisiae*, *Bifidobacterium*, and *Lactobacillus acidophilus* is 2:3:1.5 = 4:6:3). Therefore, the adjusted concentration is: *Saccharomyces cerevisiae* concentration = 9.5 × 10⁻⁶. 8 ×(4 / 13)≈2.9×10 8 CFU / g, Bifidobacterium concentration = 9.5 × 10⁻⁶ 8 ×(6 / 13)≈4.4×10 8 CFU / g, Lactobacillus acidophilus concentration = 9.5 × 10 8 ×(3 / 13)≈2.2×10 8 (The CFU / g ratio will be adjusted accordingly, and the total bacterial concentration will remain unchanged, while the other components and dosages will remain the same.)

[0132] Comparative Example 7

[0133] Compared with Example 1, the compound strain lacks brewer's yeast, and the other strains and their amounts are adjusted accordingly to keep the total strain concentration unchanged, while the other components and their amounts remain the same.

[0134] Comparative Example 8

[0135] Compared with Example 1, the compound strain lacks Bifidobacterium, and the other strains and their amounts are adjusted accordingly to maintain the total strain concentration unchanged, while the other components and their amounts remain the same.

[0136] Comparative Example 9

[0137] Compared with Example 1, the compound strain lacked Lactobacillus acidophilus, and the other strains and their amounts were adjusted accordingly to maintain the total strain concentration unchanged, while the other components and their amounts remained the same.

[0138] Comparative Example 10

[0139] Compared with Example 1, the liposomes lacked sphingomyelin, and the other components and their amounts were adjusted accordingly to keep the total liposome mass unchanged, while the other components and their amounts remained the same.

[0140] Comparative Example 11

[0141] Compared with Example 1, the liposomes lacked ascorbate palmitate, and the other components and their amounts were adjusted accordingly to keep the total liposome mass unchanged, while the other components and their amounts remained the same.

[0142] Performance testing

[0143] 1. Particle size distribution and zeta potential detection

[0144] The particle size distribution and zeta potential of the compositions of Examples 1-3 and Comparative Examples 1-11 were measured using a dynamic light scattering instrument (Malvin Zetasizer Nano ZS). The results are shown in Table 1 below:

[0145] Table 1

[0146] sample Particle size (nm) Zeta potential (mV) Example 1 120.5±5.3 -28.5±2.1 Example 2 118.7±4.8 -27.8±1.9 Example 3 122.3±6.1 -29.2±2.3 Comparative Example 1 156.8±8.2 -15.6±1.5 Comparative Example 2 162.3±7.9 -16.3±1.8 Comparative Example 3 159.7±8.5 -14.9±1.3 Comparative Example 4 165.2±9.1 -17.2±2.0 Comparative Example 5 210.3±10.5 -10.2±1.2 Comparative Example 6 185.6±9.8 -20.5±1.7 Comparative Example 7 192.3±10.2 -21.3±1.9 Comparative Example 8 188.7±9.5 -22.1±2.2 Comparative Example 9 195.4±10.8 -23.5±2.5 Comparative Example 10 145.6±7.3 -25.6±2.0 Comparative Example 11 135.8±6.7 -26.8±1.8

[0147] 2. Storage stability testing

[0148] The compositions of Examples 1-3 and Comparative Examples 1-11 were stored in constant temperature incubators at 4°C, 25°C, and 40°C for 3 months, respectively. Their appearance changes were observed, and their pH value and active ingredient content were measured. The results are shown in Table 2 below.

[0149] Table 2

[0150]

[0151]

[0152] The test data showed that the composition of Example 1, after being stored at different temperatures for 3 months, exhibited a uniform appearance, no stratification, no precipitation, minimal pH change, and stable total flavonoid content, indicating good storage stability. In contrast, the compositions of Comparative Examples 1-11 all showed varying degrees of stratification and precipitation during storage, with significant pH fluctuations and a marked decrease in the content of active ingredients. This further demonstrates the importance of the synergistic effect of the four plant extracts in the composition of this invention, the fermentation extraction method using compound microorganisms, and the rational composition of liposomes.

[0153] 3. Light stability test

[0154] The compositions of Example 1 and Comparative Examples 1-11 were placed in a light chamber and irradiated with a light intensity of 4000 lx for 15 days. Their appearance changes were observed and their particle size was measured. The results are shown in Table 3 below:

[0155] Table 3

[0156] sample Appearance Particle size (nm) Example 1 Uniform, no discoloration, no layering 122.3±5.5 Comparative Example 1 Slight discoloration, layering 165.8±8.7 Comparative Example 2 Slight discoloration, layering 172.3±9.2 Comparative Example 3 Slight discoloration, layering 168.7±8.9 Comparative Example 4 Slight discoloration, layering 175.2±9.5 Comparative Example 5 Obvious discoloration, severe layering 220.3±11.2 Comparative Example 6 Slight discoloration, layering 195.6±10.3 Comparative Example 7 Slight discoloration, layering 202.3±10.7 Comparative Example 8 Slight discoloration, layering 198.7±10.1 Comparative Example 9 Slight discoloration, layering 205.4±11.3 Comparative Example 10 Slight discoloration, layering 155.6±7.8 Comparative Example 11 Slight discoloration, layering 145.8±7.2

[0157] As shown in the table, the composition of Example 1 showed no significant change in appearance after 15 days of light irradiation, and the particle size remained basically the same as before irradiation, indicating that it has good light stability. In contrast, the compositions of Comparative Examples 1-11 all exhibited discoloration, stratification, increased particle size, and decreased stability after light irradiation, further verifying the superiority of the compositions of the present invention.

[0158] 4. Anti-wrinkle effect test

[0159] In vitro cell experiments were conducted using human skin fibroblasts as a model to determine the effects of the compositions in Examples 1-3 and Comparative Examples 1-11 on collagen secretion by cells. Cells were seeded in 96-well plates and cultured for 24 hours. Different concentrations of the compositions (diluted 1:100) were then added, and the cells were cultured for another 48 hours. The collagen content in the cell culture supernatant was measured using ELISA. The results are shown in Table 4 below (with the collagen content of the blank control group being 100%).

[0160] Table 4

[0161] sample Collagen content (%) Example 1 185.6±5.3 Example 2 182.3±4.8 Example 3 188.7±6.1 Comparative Example 1 152.3±5.8 Comparative Example 2 155.6±6.2 Comparative Example 3 150.7±5.5 Comparative Example 4 158.2±6.5 Comparative Example 5 120.3±4.2 Comparative Example 6 165.6±5.9 Comparative Example 7 168.3±6.3 Comparative Example 8 162.7±5.7 Comparative Example 9 160.4±5.4 Comparative Example 10 175.6±6.0 Comparative Example 11 172.8±5.8

[0162] Experimental results showed that the compositions of Examples 1-3 significantly promoted collagen secretion by human skin fibroblasts, with collagen content increasing by more than 80% compared to the blank control group, indicating excellent anti-wrinkle effects. However, Comparative Examples 1-4 showed a significantly reduced anti-wrinkle effect due to the lack of one plant extract; Comparative Example 5, which used solvent extraction to prepare the plant extract, showed even worse results; Comparative Examples 6-9 also showed reduced anti-wrinkle effects due to the lack of one of the complex bacterial strains; and Comparative Examples 10-11, due to changes in liposome composition, were less effective than the examples, fully demonstrating the importance of the synergistic effect between the components of the compositions of the present invention in achieving anti-wrinkle effects.

[0163] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention in any way. Although the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art can make some modifications or alterations to the above-disclosed technical content to create equivalent embodiments without departing from the scope of the present invention. Any simple modifications, equivalent changes and alterations made to the above embodiments based on the technical essence of the present invention without departing from the scope of the present invention shall still fall within the scope of the present invention.

Claims

1. A composition containing anti-wrinkle liposomes for use in cosmetic preparation, characterized in that, The composition comprises the following components by weight: 10-30 parts liposomes, 5-15 parts echinacea extract, 5-15 parts calendula extract, 5-15 parts sage extract, 5-15 parts elderberry extract, 5-20 parts moisturizer, 1-10 parts emulsifier, 0.1-1 part preservative, 0.1-1 part pH adjuster, and 20-60 parts deionized water.

2. The composition according to claim 1, characterized in that, The liposomes are composed of the following components in parts by weight: 50-70 parts lecithin, 10-30 parts cholesterol, 10-20 parts sphingomyelin, and 1-5 parts ascorbyl palmitate.

3. The composition according to claim 1, characterized in that, The purple coneflower extract, calendula extract, sage extract, and elderberry extract are all extracted by fermentation using a compound microbial strain after grinding the plants. The compound microbial strain includes Lactobacillus plantarum, Saccharomyces cerevisiae, Bifidobacterium, and Lactobacillus acidophilus.

4. The composition according to claim 3, characterized in that, The concentration of *Lactobacillus plantarum* in the compound strain is 1×10⁻⁶. 8 -5×10 8 CFU / g plant-based raw material, brewer's yeast concentration of 5×10 7 -3×10 8 CFU / g plant material, Bifidobacterium concentration is 1×10 8 -4×10 8 The concentration of Lactobacillus acidophilus in CFU / g plant raw material is 5×10⁻⁶. 7 -2×10 8 CFU / g plant-based raw materials.

5. The composition according to claim 3, characterized in that, The ratio of the strains is Lactobacillus plantarum: Saccharomyces cerevisiae: Bifidobacterium: Lactobacillus acidophilus = (2-4):(1-3):(2-3):(1-2).

6. The composition according to claim 1, characterized in that, The moisturizer is one or a combination of glycerin, propylene glycol, and 1,3-butanediol.

7. The composition according to claim 1, characterized in that, The emulsifier is one or a combination of polysorbate-80, lecithin, and glyceryl stearate.

8. The composition according to claim 1, characterized in that, The preservative is one or more of phenoxyethanol, potassium sorbate, and methylparaben.

9. The composition according to claim 1, characterized in that, The pH adjuster is one or a combination of citric acid, lactic acid, and triethanolamine.

10. A method for preparing the composition according to any one of claims 1-9, characterized in that, Includes the following steps: W1. Preparation of plant extracts; W2. Preparation of liposomes; W3. The composition is obtained by mixing liposomes, plant extracts, moisturizers, emulsifiers, preservatives, pH adjusters and deionized water.

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