Composition with long-acting moisturizing and repairing effects as well as preparation method and application thereof

Abstract

The invention relates to the technical field of cosmetics, and particularly discloses a composition with long-acting moisturizing and repairing effects and application thereof. The composition is prepared from radix gentianae macrophyllae extract, mussel extract, leontopodium alpinum flower / leaf extract and rhizoma anemarrhenae root extract according to the mass ratio of 1: (0.5 to 1.5): (0.7 to 0.9): (0.1 to 0.3). According to the composition, through a multi-path synergistic effect, the gentiana macrophylla extract reduces water loss, the mussel extract constructs a dermis water storage network, the leontopodium alpinum extract enhances water locking and barrier repairing, and the rhizoma anemarrhenae root extract improves the water content of a cuticle. Experiments show that the gentiana macrophylla extract, the mussel extract, the leontopodium alpinum flower / leaf extract and the rhizoma anemarrhenae root extract are combined according to the mass ratio of 1: 1: 0.8: 0.2 for use, and the 8-hour moisturizing rate of the composition is as high as 79.69%. The composition can be added into cosmetics such as essence, emulsion and the like, and has high moisture retention, barrier repairing power and safety.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, specifically to a composition with long-lasting moisturizing and repairing effects, its preparation method, and its application. Background Technology

[0002] Current moisturizing products in the cosmetics industry are mostly limited to epidermal hydration, failing to simultaneously achieve long-term dermal hydration and barrier repair. While existing single moisturizing ingredients (such as hyaluronic acid) can temporarily increase the stratum corneum's moisture content, they cannot effectively reduce transepidermal water loss (TEWL) or repair damaged barrier structures. Furthermore, skin dryness caused by environmental stimuli (such as UV radiation and inflammatory responses) has not yet been systematically addressed. The market urgently needs a multi-effect combination that can synergistically work on stratum corneum hydration, dermal water-retaining network construction, and barrier integrity repair to overcome the technical shortcomings of short-lasting moisturizing effects and limited repair dimensions. Summary of the Invention

[0003] Based on the solution to the above-mentioned technical problems, the present invention proposes a technical solution, specifically providing a composition with long-lasting moisturizing and repairing effects and its application. The product provided by the present invention can simultaneously replenish the epidermis with timely hydration and retain moisture in the dermis for a long time, solving the causes of dryness at different levels, and also has a certain soothing effect.

[0004] The composition of this invention with long-lasting moisturizing and repairing effects includes Gentiana macrophylla extract, mussel extract, Edelweiss flower / leaf extract, and Anemarrhena asphodeloides root extract.

[0005] The triterpenoids in Gentiana macrophylla extract can enhance the adhesion between skin keratinocytes and reduce transepidermal water loss. Furthermore, its flavonoids can inhibit the release of pro-inflammatory factors such as TNF-α and IL-6, soothing the skin and reducing redness caused by external factors (UV radiation, environmental pollution). The small molecule peptides in mussel extract can penetrate deep into the stratum corneum, forming a three-dimensional water-retaining network and prolonging water retention time. These peptides can also reduce the release of UV-induced inflammatory mediators, preventing accelerated water loss after sun exposure. The plant polysaccharides in Edelweiss flower / leaf extract can lock in water through hydrogen bonds, enhancing epidermal cell function. It has a strong ability to lock in water, and the triterpenoids contained in the flower / leaf extract of Edelweiss can stimulate keratin synthesis, repair the dense structure of the stratum corneum, and improve the integrity of the barrier. In addition, the Edelweiss polyphenols contained in it can reduce the damage of the barrier function caused by the inflammatory response, further enhancing the barrier repair effect. The Anemarrhena asphodeloides root extract contains a large number of hydrophilic hydroxyl groups, which can absorb environmental moisture and form a hydration film, directly increasing the water content of the stratum corneum. At the same time, it promotes the synthesis of filaggrin, enhances the content of natural moisturizing factors in epidermal cells, and the flavonoids in the Anemarrhena asphodeloides extract can neutralize reactive oxygen species, scavenge free radicals, and avoid skin dryness caused by oxidative stress.

[0006] The composition with long-lasting moisturizing and repairing effects provided by the present invention includes Gentiana macrophylla extract, mussel extract, Edelweiss flower / leaf extract, and Anemarrhena asphodeloides root extract in a mass ratio of 1:0.5-1.5:0.7-0.9:0.1-0.3.

[0007] Furthermore, the mass ratio of the Gentiana macrophylla extract, mussel extract, Edelweiss flower / leaf extract, and Anemarrhena asphodeloides root extract is 1:0.5-1:0.7-0.8:0.1-0.2.

[0008] Furthermore, the mass ratio of the Gentiana macrophylla extract, mussel extract, Edelweiss flower / leaf extract, and Anemarrhena asphodeloides root extract is 1:1:0.8:0.2.

[0009] The present invention also provides the use of the above composition in the preparation of cosmetics with moisturizing effects.

[0010] The present invention also provides a moisturizing cosmetic, wherein the cosmetic contains the above-mentioned composition having long-lasting moisturizing and repairing effects and a cosmetic base.

[0011] Furthermore, the moisturizing cosmetics include emulsions, creams, powders, and sprays.

[0012] Furthermore, the moisturizing cosmetics include makeup or cleansing cosmetics.

[0013] Furthermore, the moisturizing cosmetics include products such as serums, lotions, facial cleansers, shampoos, toners, makeup removers, face masks, foundations, creams, and sprays.

[0014] Furthermore, the cosmetic matrix includes at least one of solvents, moisturizers, emollients, skin conditioning agents, thickeners, emulsifiers, preservatives, pH adjusters, and chelating agents.

[0015] Furthermore, the composition with long-lasting moisturizing and repairing effects is added to moisturizing cosmetics in an amount of 0.1-10 wt%.

[0016] Furthermore, the moisturizer includes at least one of propylene glycol, glycerin, ethylene glycol, butylene glycol, hexanediol, and sorbitol.

[0017] Furthermore, the emollient includes at least one of polydimethylsiloxane, grape seed oil, camellia oil, and jojoba oil.

[0018] Furthermore, the skin conditioning agent includes at least one of squalane, plant extracts, trehalose, and dipotassium glycyrrhizate.

[0019] Furthermore, the thickener includes at least one of ammonium acryloyldimethyl taurate / VP copolymer, xanthan gum, carrageenan, and gellan gum.

[0020] Furthermore, the emulsifier includes at least one of inulin lauryl carbamate, Tween, rhamnolipid, and amino acid-based surfactants.

[0021] Furthermore, the preservative includes at least one of hydroxyacetophenone, sodium benzoate, methylparaben, and ethylparaben.

[0022] Furthermore, the pH adjuster includes at least one of organic acids and inorganic acids.

[0023] Furthermore, the chelating agent includes at least one of citrate, EDTA, and their salts.

[0024] Furthermore, the solvent includes deionized water.

[0025] Further, based on the mass percentage of the moisturizing cosmetic, the cosmetic matrix comprises 5-20% moisturizer, 1-10% emollient, 1-4% skin conditioner, 0.1-2% thickener, 1.5-5% emulsifier, 0.1-0.6% preservative, 0-0.6% pH adjuster, 0-0.7% chelating agent, and water.

[0026] Beneficial effects: The composition of this invention achieves barrier strengthening and long-lasting moisturizing through the synergistic function of the following ingredients: I. Synergistic Repair of Barrier Structures: 1. Gentiana macrophylla extract enhances the adhesion between keratinocytes and reduces transepidermal water loss; 2. Triterpenoids in the extracts of Edelweiss flowers / leaves stimulate keratin synthesis and repair the dense structure of the stratum corneum; 3. Anemarrhena asphodeloides root extract promotes filaggrin synthesis and increases the content of natural moisturizing factors in epidermal cells; Together, these three elements enhance the integrity of the barrier, forming a physical water-locking defense line.

[0027] II. Synergistic effect of active moisturizing mechanism: 1. The plant polysaccharides in the extract of Edelweiss flower / leaf enhance the water-locking capacity of epidermal cells through hydrogen bonds; 2. The small molecule peptides of mussel extract penetrate deep into the stratum corneum to form a three-dimensional water-retaining network, prolonging the time moisture stays in the skin; 3. The polysaccharides in Anemarrhena asphodeloides root extract absorb moisture from the environment to form a hydration film, directly increasing the moisture content of the stratum corneum; The three components form a three-dimensional moisturizing network that "locks in moisture at a shallow level → stores moisture at a deep level → directly replenishes moisture".

[0028] III. Core of Overall Component Enhancement: Gentian root extract, mussel extract, edelweiss flower / leaf extract, and anemarrhena root extract are combined in a specific mass ratio (i.e., 1:(0.5-1.5):(0.7-0.9):(0.1-0.3)). Gentian root extract and edelweiss flower / leaf extract work by repairing the barrier structure to reduce water loss; mussel extract and anemarrhena root extract work by capturing and storing water to increase water content. They synergistically cover the dual pathway of "plugging leaks (barrier repair) → water storage (moisturizing network)" to achieve long-lasting moisturizing and barrier strengthening effects that cannot be achieved by a single ingredient. Detailed Implementation

[0029] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0030] The technical solution of the present invention will be further described in detail below with reference to specific embodiments. It should be understood that the following embodiments are only used to explain the present invention and are not intended to limit the present invention.

[0031] Unless otherwise specified, all raw materials, reagents, instruments and equipment used in this invention can be purchased from the market or prepared by existing methods.

[0032] In a specific embodiment of the present invention, the source of the raw materials is as follows: Polydimethylsiloxane: Purchased from Jiangxi Lanxing Xinghuo Organosilicon Co., Ltd., trade name: MIRASIL DM 10.

[0033] Squalane: Purchased from Senris Biotechnology (Shenzhen) Co., Ltd., trade name: Yeast Squalane.

[0034] Acryloyldimethyl taurate ammonium / VP copolymer: purchased from Clariant Chemicals (Shanghai) Co., Ltd., trade name: Aristoflex AVC.

[0035] Xanthan Gum: Purchased from CP Kelco Singapore Pte, Ltd., product name: KELTROL® CG LAX-TXANTHAN GUM.

[0036] Inulin lauryl carbamate: purchased from Gobiotics BV, brand name: INUTEC SL1.

[0037] Gentiana macrophylla extract: purchased from Zhejiang Zhongke Zhongzhi Biotechnology Co., Ltd., trade name: Tangola.

[0038] Mussel extract: purchased from Baiyin Biotechnology Co., Ltd., trade name: MAP-Reno, solid content: 0.5%.

[0039] Alpine edelweiss flower / leaf extract: purchased from DSM Nutritional Products Ltd., brand name: ALPAFLOR® EDELWEISS.

[0040] Anemarrhena asphodeloides root extract: purchased from SEDERMA, SAS, trade name: Volufiline.

[0041] The raw materials and their mass ratios, as well as the preparation method, of the composition with long-lasting moisturizing and repairing effects in this invention are as follows: Composition 1: It is composed of Gentiana macrophylla extract, mussel extract, Edelweiss flower / leaf extract, and Anemarrhena asphodeloides root extract in a mass ratio of 1:1:0.8:0.2. Its preparation method is as follows: Gentiana macrophylla extract, mussel extract, Edelweiss flower / leaf extract, and Anemarrhena asphodeloides root extract are mixed evenly in the mass ratio, sealed and stored in the dark.

[0042] Composition 2: It is composed of Gentiana macrophylla extract, mussel extract, Edelweiss flower / leaf extract, and Anemarrhena asphodeloides root extract in a mass ratio of 1:1.5:0.7:0.1; and its preparation method is the same as that of composition 1.

[0043] Composition 3: It is composed of Gentiana macrophylla extract, mussel extract, Edelweiss flower / leaf extract, and Anemarrhena asphodeloides root extract in a mass ratio of 1:0.5:0.6:0.3; and its preparation method is the same as that of composition 1.

[0044] Composition ①: Unlike composition 1, it lacks Gentiana macrophylla extract. The missing mass is made up by mussel extract, Edelweiss flower / leaf extract, and Anemarrhena asphodeloides root extract in a mass ratio of 1:0.8:0.2. The preparation method is as follows: the mussel extract, Edelweiss flower / leaf extract, and Anemarrhena asphodeloides root extract are mixed evenly in the mass ratio and stored in a sealed container away from light.

[0045] Composition ②: Unlike composition 1, it lacks mussel extract. The missing mass is made up by extracts of Gentiana macrophylla, Edelweiss flower / leaf extract, and Anemarrhena asphodeloides root extract in a mass ratio of 1:0.8:0.2. The preparation method is as follows: Gentiana macrophylla extract, Edelweiss flower / leaf extract, and Anemarrhena asphodeloides root extract are mixed evenly in the mass ratio and stored in a sealed container away from light.

[0046] Composition ③: Unlike composition 1, it lacks the extract of Edelweiss flower / leaf. The missing mass is made up by the extracts of Gentiana macrophylla, Mussel, and Anemarrhena asphodeloides root in a mass ratio of 1:1:0.2. The preparation method is as follows: the extracts of Gentiana macrophylla, Mussel, and Anemarrhena asphodeloides root are mixed evenly according to the mass ratio and stored in a sealed container away from light.

[0047] Composition ④: Unlike composition 1, it lacks Anemarrhena asphodeloides root extract. The missing mass is made up by Gentiana macrophylla extract, mussel extract, and Edelweiss flower / leaf extract in a mass ratio of 1:1:0.8. The preparation method is as follows: Gentiana macrophylla extract, mussel extract, and Edelweiss flower / leaf extract are mixed evenly in the mass ratio and stored in a sealed container away from light.

[0048] Composition ⑤: It is composed of Gentiana macrophylla extract, mussel extract, Edelweiss flower / leaf extract, and Anemarrhena asphodeloides root extract in a mass ratio of 1:0.2:1:0.8; and its preparation method is the same as that of composition 1.

[0049] Composition performance testing: The moisturizing effects of compositions 1-3 and compositions ①-⑤ were evaluated. (1) Test principle: Based on the different forces that different moisturizers exert on water molecules, their ability to absorb and retain water also varies slightly. The moisturizing rate of a moisturizer can be characterized by testing it in the laboratory (weighing method).

[0050] (2) Instruments and materials: Instrument: Constant temperature and humidity chamber; Test samples: Compositions 1-3, ①-⑤; Preparation of sample solutions: Each test sample was prepared into a 10wt% mixture using deionized water; Test environment conditions: The test environment temperature is 20℃±1℃ and the humidity is 45±2%.

[0051] Test method: Weigh 700 μL of sample solution and spread it on a glass slide. Place the glass slide in a constant temperature and humidity chamber. Weigh the glass slide at the 8th hour, record the data and calculate the moisture retention rate.

[0052] Test result calculation: According to the experimental design, M0 (initial slide mass after adding sample), Mglass (slide mass itself), and Mt (slide mass after corresponding storage time) were measured respectively. The moisture retention rate was then calculated using the formula: Moisturizing rate = (Mt - Mglass) / (M0 - Mglass) × 100% The moisturizing rate of each test substance was calculated.

[0053] The experimental results are shown in Table 1.

[0054] Table 1 Effect of composition on moisturizing rate Group 8h relative hydration rate / % Composition 1 79.69 Composition 2 74.37 Composition 3 75.21 Composition ① 57.34 Composition ② 62.09 Composition ③ 59.26 Composition ④ 60.37 Composition ⑤ 65.56 As can be seen from the data in Table 1, compared with compositions ①-⑤, compositions 1-3 of the present invention, which have long-lasting moisturizing and repairing effects, have superior moisturizing efficacy. Specifically, comparing the results of composition 1 with those of compositions ①-④, it can be seen that the combined use of Gentiana macrophylla extract, mussel extract, Edelweiss flower / leaf extract, and Anemarrhena asphodeloides root extract has a synergistic effect on moisturizing efficacy. Comparing the results of composition 1 with those of composition ⑤, it can be seen that the mass ratio of Gentiana macrophylla extract, mussel extract, Edelweiss flower / leaf extract, and Anemarrhena asphodeloides root extract in the composition has a certain influence on the moisturizing efficacy. When Gentiana macrophylla extract, mussel extract, Edelweiss flower / leaf extract, and Anemarrhena asphodeloides root extract are combined according to the mass ratio specified in the present invention, the resulting composition has better moisturizing efficacy.

[0055] Human skin test Test samples: Compositions 1-3 and ①-⑤ were compounded with the moisturizing essence base to form moisturizing essences 1-3 and ①-⑤, respectively; wherein the mass ratio of each composition to the moisturizing essence base was 3:97.

[0056] The components of the moisturizing essence base are as follows: 1,3-Propanediol: 5wt% Glycerin: 3.0 wt% 1,2-Hexanediol: 0.5 wt%; Polydimethylsiloxane: 3.0 wt% Squalane: 2.0 wt% Ammonium acryloyldimethyltaurate / VP copolymer: 0.8 wt%; Xanthan gum: 0.2 wt%; Inulin lauryl carbamate: 2.0 wt%; Hydroxyacetophenone: 0.5 wt% Deionized water to 100 wt%; The preparation method of the moisturizing essence base is as follows: S1: Add 1,3-propanediol, glycerol, 1,2-hexanediol, and hydroxyacetophenone to an aqueous phase pot, heat to 65°C, and stir at 200 rpm for 15 minutes. Keep warm and set aside for later use. S2: Deionized water, inulin lauryl carbamate, ammonium acryloyl dimethyl taurate / VP copolymer, and xanthan gum are added sequentially to the aqueous phase pot, heated to 82°C, and stirred at 150 rpm for 20 min. Keep warm for later use. S3: Add polydimethylsiloxane and squalane to an emulsifying pot, heat to 82°C, and stir at 200 rpm for 20 minutes. Keep warm and set aside for later use. S4: Set the vacuum in the emulsifying pot to -0.06MPa, start stirring at 200rpm, and draw the material from the aqueous phase pot into the emulsifying pot. After all the material has been drawn out, stir at 100rpm for 8 minutes, then stir at 200rpm for 10 minutes. Release the vacuum, start stirring, and allow it to cool naturally to room temperature to obtain the moisturizing essence matrix.

[0057] Preparation method of moisturizing essence: A1: Add the composition to the moisturizing essence base and stir at 200 rpm for 15 min; A2: Let stand at room temperature for 2 hours to obtain the moisturizing essence.

[0058] Experiment 1: Safety Test of Moisturizing Essence Using the 2015 "Cosmetic Safety Technical Specifications" as a reference standard, the safety of moisturizing essences 1-3 and ①-⑤ was evaluated. Forty people aged 16-65 years were randomly divided into groups of 5, and skin patch tests were conducted in each group.

[0059] Test method: Place the test substance into a patch applicator at a dosage of 0.020-0.025 g. Cover the patch applicator containing the test substance onto the flexor side of the subject's forearm with non-irritating adhesive tape. Gently press with the palm of your hand to ensure even adhesion to the skin surface. Leave on for 24 hours. Remove the patch applicator and observe the skin reaction 30 minutes after the pressure mark disappears. If the result is negative, observe again at 24 hours and 48 hours after the patch test.

[0060] Evaluation criteria: Grade 0: Negative reaction; Grade 1: Suspicious reaction, with only slight erythema; Grade 2: Weak positive reaction, erythema, infiltration, edema, and possible papules; Grade 3: Strong positive reaction, erythema, infiltration, edema, papules may be present, and the reaction may extend beyond the test area; Grade 4: Extremely positive reaction, with obvious erythema, severe infiltration, edema, confluent herpes, and reaction extending beyond the test area.

[0061] Test results: All subjects had negative skin reactions.

[0062] The above test results show that the moisturizing essence provided by the present invention is gentle and non-irritating to the skin and is safe to use.

[0063] Experiment 2: The effect of moisturizing essence on transepidermal water loss from the skin Experimental Methods: Forty-eight volunteers aged 20-35 years with similar skin conditions were randomly divided into eight groups of six (three men and three women) each. Every evening, after washing their faces with water, each group applied 1.5g of the aforementioned moisturizing essence evenly to their face for 40 consecutive days. A transepidermal water loss analyzer (CK, Germany) was used to collect skin moisture data on the cheeks before and after 40 days of essence use, under ambient conditions of 25±1℃ and 55±2% humidity. The average transepidermal water loss value (C0) before essence use, the average transepidermal water loss value (C40) after 40 days of essence use, and the reduction rate (γ) of transepidermal water loss value after 40 days of essence use were calculated. The results of the transepidermal water loss rate performance test are shown in Table 2.

[0064] Reduction rate Y (%) = (C0 - C40) / C0 × 100% Table 2. Effects of moisturizing serums on transepidermal water loss Group <![CDATA[C0 / (g / h·m 2 )]]> <![CDATA[C40 / (g / h·m 2 )]]> Y / （%） Moisturizing Essence 1 12.16 7.85 35.44 Moisturizing Essence 2 11.02 7.23 34.39 Moisturizing Essence 3 11.51 7.57 34.23 Moisturizing Essence ① 10.49 8.01 23.64 Moisturizing Essence ② 11.09 8.34 24.80 Moisturizing Essence ③ 11.25 8.88 21.07 Moisturizing Essence ④ 12.02 9.21 23.38 Moisturizing Essence ⑤ 11.17 7.87 29.54 As shown in Table 2, the test results indicate that moisturizing essences 1-3 can significantly reduce transepidermal water loss and have a certain effect on repairing the skin barrier. Comparing the results of moisturizing essence 1 with those of moisturizing essences ①-④, it can be seen that the extracts of Gentiana macrophylla, mussels, Edelweiss flower / leaf extract, and Anemarrhena asphodeloides root extract have a significant synergistic effect. When used together, they can significantly reduce transepidermal water loss. Comparing the results of moisturizing essence 1 with those of moisturizing essence ⑤, it can be seen that the combination of Gentiana macrophylla extract, mussels, Edelweiss flower / leaf extract, and Anemarrhena asphodeloides root extract in a specific mass ratio can effectively reduce transepidermal water loss.

[0065] The embodiments described above are some, but not all, embodiments of the present invention. The detailed description of the embodiments of the present invention is not intended to limit the scope of the claimed invention, but merely to illustrate selected embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without inventive effort are within the scope of protection of the present invention.

Claims

1. A composition with long-lasting moisturizing and repairing effects, characterized in that, The composition includes Gentiana macrophylla extract, mussel extract, Edelweiss flower / leaf extract, and Anemarrhena asphodeloides root extract, wherein the mass ratio of Gentiana macrophylla extract, mussel extract, Edelweiss flower / leaf extract, and Anemarrhena asphodeloides root extract is 1:0.5-1.5:0.7-0.9:0.1-0.

3.

2. The composition according to claim 1, characterized in that, The mass ratio of the Gentiana macrophylla extract, mussel extract, Edelweiss flower / leaf extract, and Anemarrhena asphodeloides root extract is 1:0.5-1:0.7-0.8:0.1-0.

2.

3. The composition according to claim 1, characterized in that, The mass ratio of the Gentiana macrophylla extract, mussel extract, Edelweiss flower / leaf extract, and Anemarrhena asphodeloides root extract is 1:1:0.8:0.

2.

4. The use of a composition having long-lasting moisturizing and repairing effects as described in any one of claims 1-3 in the preparation of a cosmetic having moisturizing effects.

5. A moisturizing cosmetic, characterized in that, The cosmetic contains the composition with long-lasting moisturizing and repairing effects as described in any one of claims 1-3, and a cosmetic base.

6. The moisturizing cosmetic according to claim 5, characterized in that, The moisturizing cosmetics include lotions, creams, powders, and sprays.

7. The moisturizing cosmetic according to claim 5, characterized in that, The cosmetic base includes at least one of the following: solvent, moisturizer, emollient, skin conditioner, thickener, emulsifier, preservative, pH adjuster, and chelating agent.

8. The moisturizing cosmetic according to claim 5, characterized in that, The composition with long-lasting moisturizing and repairing effects is added to moisturizing cosmetics at a rate of 0.1-10 wt%.