A mouse brain embedding box for neuroscience research and a low-temperature storage device thereof

Abstract

The application belongs to the technical field of neuroscience research, and particularly relates to a mouse brain embedding box for neuroscience research and a low-temperature storage device thereof. The mouse brain embedding box comprises a storage cabinet, a separation assembly arranged in the storage cabinet, a refrigeration assembly arranged in the storage cabinet, a plurality of placing assemblies arranged in the storage cabinet, the plurality of placing assemblies being respectively located at the top of a plurality of second partitions, and a plurality of gas storage assemblies arranged in the storage cabinet. The embedding box is stored in a storage drawer, so that the whole is not exposed when a single embedding box is stored or accessed, and cold air leakage is effectively reduced. When the storage drawer is pulled out, the cold air is stored in a gas storage tank by the gas storage assembly, and when the storage drawer is retracted, the cold air in the gas storage tank is sent back to the storage drawer by the gas storage assembly, so that the cold air can be recycled, and the effect of reducing cold air waste is achieved. The problem of unnecessary energy waste caused by the existing refrigeration device in the process of taking the embedding box is solved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of neuroscience research, and particularly relates to a mouse brain embedding box for neuroscience research and a low-temperature storage device thereof. BACKGROUND

[0002] In the field of neuroscience research, especially in brain science research based on animal models, rats are important experimental objects due to the similarity between their nervous systems and those of humans. Cryosection of mouse brain tissue is a key preparatory step for subsequent research such as morphological observation, immunohistochemistry, and in situ hybridization. In this process, tissue embedding and low-temperature storage are core links to ensure the quality of the sections.

[0003] Currently, researchers usually place the embedded mouse brain samples in a dedicated plastic embedding box, and then store a large number of embedding boxes in a low-temperature environment provided by a large ultra-low temperature refrigerator or a liquid nitrogen tank. However, the current storage method has a significant technical pain point: the mainstream low-temperature storage equipment (such as cryogenic shelves and cryogenic boxes) is designed in a whole or drawer structure. When researchers need to frequently access one or several specific mouse brain samples, they often have to take out the entire storage unit (even the entire drawer or shelf) from the low-temperature environment, expose it to room temperature, and search and take out the samples.

[0004] This operation mode causes several serious drawbacks: first, it causes a large amount of cold air to quickly escape from the storage equipment, and a large amount of external hot air to invade, which not only causes a sharp fluctuation and rebound of the temperature in the equipment, but also poses a serious threat to the preservation quality of all stored samples (especially those not taken out). Repeated temperature shocks can accelerate sample degradation and affect the accuracy and repeatability of research data. Second, the compressor or liquid nitrogen consumption system needs to work overload to quickly restore the set low-temperature environment, which consumes a large amount of energy and causes unnecessary energy waste, increasing the operating cost of the laboratory. SUMMARY

[0005] To solve the above technical problems, the present application provides a mouse brain embedding box for neuroscience research and a low-temperature storage device thereof, which stores the embedding boxes in the storage drawer separately, avoids exposing the whole device when accessing a single embedding box, effectively reduces the escape of cold air, stores the cold air in the gas storage tank through the gas storage assembly when the storage drawer is extracted, and sends the cold air in the gas storage tank back to the storage drawer through the gas storage assembly when the storage drawer is retracted, so that the cold air can be recycled, achieving the effect of reducing the waste of cold air.

[0006] The technical solution for achieving the purpose of the present application is: a low-temperature storage device of a mouse brain embedding box for neuroscience research, comprising a storage cabinet, an internal part of the storage cabinet is provided with a partition assembly, the partition assembly comprises:

[0007] The first partition is fixedly installed in the middle of the inner side wall of the storage cabinet, and a plurality of second partitions are fixedly installed on the left and right side walls of the first partition at equal intervals. The side walls of the plurality of second partitions are fixedly connected with the inner side wall of the storage cabinet;

[0008] The plurality of air dispersing holes are respectively arranged in the inner side wall of the second partition, and the air dispersing holes are located inside the second partition and close to the outside;

[0009] The inner part of the storage cabinet is provided with a refrigeration assembly, and the refrigeration assembly comprises:

[0010] The refrigeration device is fixedly installed on the inner bottom wall of the storage cabinet;

[0011] The air pipe is arranged in the inner part of the first partition, the air pipe is in communication with the plurality of air dispersing holes, and the inner part of the air pipe is in communication with the output end of the refrigeration device;

[0012] The inner part of the storage cabinet is provided with a plurality of placing assemblies, and the plurality of placing assemblies are respectively located on the top of the plurality of second partitions. The placing assembly comprises:

[0013] The storage drawer is slidingly installed on the top wall of the second partition, the front side wall of the storage drawer is fixedly installed with a pull ring, and the bottom wall of the storage drawer is provided with an air inlet hole, which is aligned with the adjacent air dispersing hole;

[0014] The inner part of the storage cabinet is provided with a plurality of gas storage assemblies, and the plurality of gas storage assemblies are respectively located on the rear side of the plurality of placing assemblies.

[0015] In some embodiments, the gas storage assembly comprises:

[0016] The gas storage tank is fixedly installed on the top wall of the second partition, two third partitions are fixedly installed on the inner side wall of the gas storage tank close to the storage drawer, the upper and lower side walls of the two third partitions are fixedly connected with the upper and lower inner side walls of the gas storage tank, a certain gap is left between the two third partitions and the inner side wall of the gas storage tank away from the storage drawer, a piston plate is slidingly installed in the inner part of the gas storage tank, the left and right side walls of the piston plate are attached to the side wall close to the two third partitions, a pull rod is fixedly installed on the side wall of the piston plate, and the end of the pull rod away from the piston plate is slidingly penetrated through the side wall of the gas storage tank and fixedly connected with the storage drawer;

[0017] The communication pipe is slidably penetrated through the side wall of the storage drawer, and the plurality of communication pipes are aligned with the cavities formed by the two partition plates and the inner side wall of the storage cabinet.

[0018] In some embodiments, the placing assembly further comprises:

[0019] The track slot is internally provided with a folding plate fixedly connected to the side wall away from the outside, the left and right ends of the folding plate are slidably installed at the corresponding positions in the track slot, a positioning strip is slidably installed in the track slot, the positioning strip is fixedly connected to the corresponding side wall of the folding plate, and a tension strip is fixedly installed on the top wall of the positioning strip.

[0020] In some embodiments, a sealing strip is fixedly installed on the side wall of the piston plate, the sealing strip is made of rubber, a return spring is sleeved on the side wall of the pull rod, and the two ends of the return spring are fixedly connected to the corresponding side walls of the piston plate and the gas storage tank.

[0021] In some embodiments, the storage drawer is internally provided with a supporting assembly, and the supporting assembly comprises:

[0022] The plurality of communication plates are slidably penetrated through the side wall of the storage drawer close to the gas storage tank, the outer side walls of the plurality of communication plates are slidably installed with sliding plates, the sliding plates are fixedly installed on the inner bottom wall of the storage drawer, the interiors of the communication plates and the sliding plates are hollow, the communication plates are in communication with the cavities formed by the side wall close to the two partition plates, a containing rod is fixedly installed on the side wall of the sliding plate, a containing slot is formed on the top wall of the containing rod, an extension rod is slidably installed in the containing slot, and the side walls of the containing rod and the sliding plate are provided with pressure holes in communication with each other.

[0023] In some embodiments, the top wall of the extension rod is fixedly installed with a placing plate, a plurality of air slots are formed in the side wall of the placing plate, and the embedding box is placed on the top of the placing plate.

[0024] In some embodiments, the supporting assembly further comprises:

[0025] The through hole is formed in the inner side wall of the sliding plate, and the through hole is in communication with the plurality of pressure holes in the interior of the sliding plate.

[0026] In some embodiments, the bottom wall of the extension rod is fixedly installed with a limiting strip, an air hole is formed in the bottom end of the side wall of the extension rod, and the top end of the air hole penetrates through the side wall of the extension rod and is in communication with the outside.

[0027] In some embodiments, the interior of the storage drawer is provided with a linkage assembly, which comprises:

[0028] Positioning blocks, a plurality of which are fixedly installed on the side wall of the communication pipe;

[0029] A linkage strip is fixedly installed on the bottom wall surface of the positioning strip, the bottom wall surface of the linkage strip and the top wall surface of the positioning block are both fixedly installed with magnetic blocks, and the magnetic blocks on the positioning block and the magnetic blocks on the linkage strip are magnetically different on the side wall surface close to each other.

[0030] A mouse brain embedding box for neuroscience research is stored using the above-mentioned low-temperature storage device, comprising:

[0031] A storage plate, the top wall surface of which is provided with a storage groove;

[0032] A cover plate covers the top of the storage plate, and the top wall surface of the cover plate is provided with an indicating groove in the shape of a trapezoidal groove.

[0033] Compared with the prior art, the present application has the following advantages:

[0034] Firstly, in the present application, the staff pulls out the storage drawer from the top of the second partition, and the cold air generated by the refrigeration device is injected into the interior of the storage drawer through the air inlet hole, the air vent pipe and the air outlet hole, so as to refrigerate the samples stored in the interior of the storage drawer. When the staff pulls out the storage drawer, the pull rod pulls the piston plate to move together. Since the side wall of the piston plate is in contact with the side walls of the two third partitions and the inner side wall of the storage cabinet, the piston plate can suck the cold air in the interior of the storage drawer into the cavity formed between the two third partitions through the communication hole, so as to store the cold air. When the staff pushes the storage drawer into the gas storage tank, the cold air stored between the two third partitions is sent into the interior of the communication hole through the gap between the third partition and the inner side wall of the storage cabinet by the movement of the piston plate, and then returns to the interior of the storage drawer through the communication hole, so as to recycle the cold air. By storing the embedding box in the storage drawer, the whole embedding box is not exposed when a single embedding box is accessed, the leakage of cold air is effectively reduced, the heat exchange load is reduced, energy consumption is saved, and the quality of low-temperature preservation of the samples is significantly improved. By storing the cold air in the gas storage tank when the storage drawer is pulled out, and sending the cold air in the gas storage tank back to the storage drawer when the storage drawer is retracted, the cold air can be recycled, so that the waste of cold air is reduced. The problem of unnecessary energy waste caused by the existing refrigeration device in the process of taking the embedding box is solved.

[0035] Secondly, the folding plate is slidably installed in the inside of the track groove, so that the folding plate can seal the top of the storage drawer, so that the storage drawer is in a sealed environment, avoiding the cold air in the storage drawer from spreading everywhere, which causes poor sample refrigeration effect in the storage drawer. The cooperation of the positioning strip and the pull strip enables the staff to conveniently push and pull the folding plate in the track groove, and facilitates the opening or closing operation of the top of the storage drawer, so as to conveniently put the sample into the storage drawer or take it out of the storage drawer.

[0036] Thirdly, the elasticity of the reset spring is utilized to support the side wall of the piston plate, so that the piston plate and the pull rod jointly pull the storage drawer to move towards the gas storage tank, so that the storage drawer can be automatically retracted between the two partition plates, and the sealing strip is sleeved on the side wall of the piston plate. By means of the friction between the sealing strip and the side wall of the partition plate three and the gas storage tank, the moving speed of the piston plate is effectively reduced, and the automatic retraction amplitude of the storage drawer is inhibited.

[0037] Fourthly, when the storage drawer moves outward, the position of the communication pipe is unchanged since the communication pipe is fixedly installed on the side wall of the gas storage tank. The positioning block and the linkage strip are connected together by the magnetism of the magnetic block, and the position of the positioning strip is fixed. With the movement of the storage drawer, the folding plate is folded. During the process that the storage drawer is pulled out, the folding plate is synchronously opened, which further facilitates the staff to take out the embedding box. Since the positioning block and the linkage strip are connected by the magnetism of the magnetic block, the staff can manually close or open the folding plate by pulling the pull strip.

[0038] Fifthly, the top of the storage plate has a groove part by the storage groove, so as to facilitate the positioning of the sample put into the inside of the storage plate and avoid the position of the sample from being moved everywhere. The top of the storage plate is in a sealed state by covering the top of the storage plate with the cover plate, so as to improve the protection effect of the sample. By utilizing the characteristics that one end of the trapezoid is larger and the other end is smaller, when the cover plate covers the top of the storage plate, the staff can distinguish the head and tail of the sample, avoid the head and tail of the sample from being difficult to distinguish due to freezing, and avoid the occurrence of the situation that the sample is cut by mistake, causing experimental loss. BRIEF DESCRIPTION OF DRAWINGS

[0039] The present application will be further explained in combination with the drawings and embodiments:

[0040] Figure 1 is a whole structure perspective view of the present application;

[0041] Figure 2 is a position schematic view of the gas scattering hole and the air pipe of the present application;

[0042] Figure 3 is a whole structure perspective view of the storage drawer of the present application;

[0043] Figure 4 is a perspective view of the internal structure of the storage drawer according to the present application;

[0044] Figure 5 is a perspective view of the vertical cross-section of the storage drawer and the gas storage tank according to the present application;

[0045] Figure 6 is a perspective view of the internal structure of the cover plate according to the present application;

[0046] Figure 7 is a perspective view of the horizontal cross-section of the storage drawer and the gas storage tank according to the present application;

[0047] Figure 8 is a perspective view of the internal structure of the sliding plate according to the present application;

[0048] Figure 9 is a perspective view of the internal structure of the containing rod according to the present application;

[0049] Figure 10 is a perspective view of the internal structure of the communication pipe according to the present application;

[0050] Figure 11 is a perspective view of the storage drawer according to the present application in the pulled-out state.

[0051] Explanation of reference signs:

[0052] 1, storage cabinet; 21, partition one; 22, partition two; 23, air dispersing hole; 31, refrigeration device; 32, air communication pipe; 41, storage drawer; 42, pull ring; 43, track strip; 44, track groove; 45, folding plate; 46, positioning strip; 47, pull strip; 48, air inlet hole; 51, storage plate; 52, storage groove; 53, cover plate; 54, indication groove; 61, gas storage tank; 62, partition three; 63, piston plate; 64, sealing strip; 65, pull rod; 66, return spring; 67, communication pipe; 68, communication hole; 71, communication plate; 72, sliding plate; 73, containing rod; 74, containing groove; 75, extension rod; 76, pressurizing hole; 77, through hole; 78, limiting strip; 79, air hole; 8, placement plate; 91, positioning block; 92, linkage strip; 93, magnetic block. DETAILED DESCRIPTION

[0053] The present application will be described in detail below, and the technical solutions in the embodiments of the present application will be clearly and completely described. Obviously, the described embodiments are only some of the embodiments of the present application, but not all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0054] The application provides a mouse brain embedding box for neuroscience research and a low-temperature storage device thereof.

[0055] Embodiment one

[0056] As shown in the figure, a mouse brain embedding box for neuroscience research comprises a storage plate 51 and a cover plate 53. Figures 4-6

[0057] The storage plate 51 is placed in the inside of the storage drawer 41, and a storage groove 52 is formed in the top wall of the storage plate 51; the top of the storage plate 51 has a recessed portion through the storage groove 52, so as to facilitate positioning of the sample placed in the inside of the storage plate 51 and avoid the position of the sample from being moved around.

[0058] The cover plate 53 covers the top of the storage plate 51, and an indicating groove 54 is formed in the top wall of the cover plate 53, and the indicating groove 54 is a trapezoidal groove; the top of the storage plate 51 is in a sealed state by covering the top of the storage plate 51 with the cover plate 53, so as to improve the protection effect on the sample; the trapezoidal groove has the characteristics that one end is large and the other end is small, so as to facilitate the staff to distinguish the head and tail of the sample when the cover plate 53 covers the top of the storage plate 51, avoid the head and tail of the sample from being difficult to distinguish due to freezing, and cause the sample to be cut by mistake, resulting in experimental loss.

[0059] The specific working method is as follows: the top of the storage plate 51 has a recessed portion through the storage groove 52, so as to facilitate positioning of the sample placed in the inside of the storage plate 51; the top of the storage plate 51 is in a sealed state by covering the top of the storage plate 51 with the cover plate 53, so as to improve the protection effect on the sample; the trapezoidal groove has the characteristics that one end is large and the other end is small, so as to facilitate the staff to distinguish the head and tail of the sample when the cover plate 53 covers the top of the storage plate 51, avoid the head and tail of the sample from being difficult to distinguish due to freezing, and cause the sample to be cut by mistake, resulting in experimental loss.

[0060] Embodiment two

[0061] As shown in the figure, a low-temperature storage device of a mouse brain embedding box for neuroscience research is used for storing the mouse brain embedding box in embodiment one and comprises a storage cabinet 1, and a partition assembly is arranged in the inside of the storage cabinet 1, and the partition assembly comprises a partition plate one 21 and air vents 23. Figures 1-11 The partition plate one 21 is fixedly installed at the middle position of the inner side wall of the storage cabinet 1, a plurality of partition plate twos 22 are fixedly installed at equal intervals on the left and right side walls of the partition plate one 21, and the side walls of the plurality of partition plate twos 22 are fixedly connected with the inner side wall of the storage cabinet 1.

[0062]

[0063] ​​The plurality of air vents 23 are arranged on the inner side wall of the second partition 22, and the air vents 23 are located inside the second partition 22 and close to the outside;

[0064] The inside of the storage cabinet 1 is provided with a refrigeration assembly, and the refrigeration assembly comprises a refrigeration device 31 and a ventilation pipe 32;

[0065] The refrigeration device 31 is fixedly installed on the inner bottom wall of the storage cabinet 1, and the refrigeration device 31 is used for generating cold air. The refrigeration device 31 is a prior art, and details are not repeated here.

[0066] The ventilation pipe 32 is arranged in the inside of the first partition 21, and the ventilation pipe 32 is communicated with the plurality of air vents 23, and the inside of the ventilation pipe 32 is communicated with the output end of the refrigeration device 31;

[0067] The inside of the storage cabinet 1 is provided with a plurality of placing assemblies, and the plurality of placing assemblies are respectively located on the top of the plurality of second partitions 22. The placing assembly comprises a storage drawer 41 and a track strip 43.

[0068] The storage drawer 41 is slidably installed on the top wall of the second partition 22. The top wall of the storage drawer 41 is open. The front wall of the storage drawer 41 is fixedly installed with a pull ring 42. The bottom wall of the storage drawer 41 is provided with an air inlet hole 48, and the air inlet hole 48 is aligned with the air vent 23. The pull ring 42 is used for conveniently pulling out the storage drawer 41 from the top of the second partition 22. The air inlet hole 48 is used for conveniently injecting the cold air generated by the refrigeration device 31 into the inside of the storage drawer 41 through the ventilation pipe 32 and the air vent 23, so as to refrigerate the samples stored in the inside of the storage drawer 41.

[0069] The track strip 43 is fixedly installed on the inner side wall top end of the storage drawer 41. The inner side wall of the track strip 43 is provided with a track groove 44. The inside of the track groove 44 is fixedly connected with a folding plate 45. The left and right ends of the folding plate 45 are slidably installed on the corresponding positions in the inside of the track groove 44. The inside of the track groove 44 is slidably installed with a positioning strip 46. The positioning strip 46 is fixedly connected with the corresponding side wall of the folding plate 45. The top wall of the positioning strip 46 is fixedly installed with a pull strip 47. The folding plate 45 is slidably installed in the inside of the track groove 44, so that the folding plate 45 can seal the top of the storage drawer 41, so that the storage drawer 41 is in a sealed environment, avoiding the cold air in the inside of the storage drawer 41 to spread everywhere, resulting in poor refrigeration effect of the samples in the inside of the storage drawer 41. The mutual cooperation of the positioning strip 46 and the pull strip 47 enables the workers to conveniently push and pull the folding plate 45 to move in the inside of the track groove 44, and also facilitates the opening or closing operation of the top end of the storage drawer 41, so as to conveniently put the samples into the inside of the storage drawer 41 or take out the samples from the inside of the storage drawer 41.

[0070] The inside of the storage cabinet 1 is provided with a plurality of gas storage assemblies, which are respectively located at the back side of the plurality of placing assemblies. The gas storage assembly comprises a gas storage tank 61 and a communication pipe 67.

[0071] The gas storage tank 61 is fixedly installed on the top wall of the second partition plate 22. Two third partition plates 62 are fixedly installed on the side wall of the inside of the gas storage tank 61 close to the storage drawer 41. The upper and lower side walls of the two third partition plates 62 are fixedly connected with the upper and lower side walls of the inside of the gas storage tank 61. A certain gap is left between the two third partition plates 62 and the side wall of the inside of the gas storage tank 61 away from the storage drawer 41. A piston plate 63 is slidingly installed in the inside of the gas storage tank 61. The left and right side walls of the piston plate 63 are attached to the side wall close to the two third partition plates 62. A sealing strip 64 is fixedly installed on the side wall of the piston plate 63. The sealing strip 64 is made of rubber. A pull rod 65 is fixedly installed on the side wall of the piston plate 63. The end of the pull rod 65 away from the piston plate 63 is slidingly penetrated through the side wall of the gas storage tank 61 and is fixedly connected with the storage drawer 41. A return spring 66 is sleeved on the side wall of the pull rod 65. The two ends of the return spring 66 are respectively fixedly connected with the side walls of the piston plate 63 and the gas storage tank 61 corresponding to each other. The elasticity of the return spring 66 is used to support the side wall of the piston plate 63. The piston plate 63 and the pull rod 65 jointly pull the storage drawer 41 to move in the direction of the gas storage tank 61, so that the storage drawer 41 can be automatically retracted between the two second partition plates 22. The sealing strip 64 is sleeved on the side wall of the piston plate 63. The friction between the sealing strip 64 and the side walls of the third partition plate 62 and the gas storage tank 61 effectively reduces the moving speed of the piston plate 63 and inhibits the automatic retraction amplitude of the storage drawer 41.

[0072] There are multiple connecting pipes 67, which are fixedly installed on both sides of the side wall of the air storage box 61 near the storage drawer 41. The end of the connecting pipe 67 away from the air storage box 61 slides through the side wall of the storage drawer 41. The multiple connecting pipes 67 are aligned with the cavities formed by the two partitions 62 and the inner side wall of the storage cabinet 1. The side wall of the connecting pipe 67 is provided with a connecting hole 68, which communicates with the interior of the storage drawer 41 and the air storage box 61. When the operator pulls the pull ring 42 to pull the storage drawer 41 outward, the pull rod 65 will pull the piston plate 63 to move together. Because the side wall of the piston plate 63 is aligned with the two partitions 62 and the inner side wall of the storage cabinet 1, the connecting pipe 67 is aligned with the inner side wall of the storage cabinet 41. The side wall of partition 3 62 is attached to the inner side wall of storage cabinet 1. Therefore, piston plate 63 can draw the cold air inside storage drawer 41 into the cavity formed between the two partitions 3 62 through the connecting hole 68 to store the cold air inside storage drawer 41. When the staff pushes storage drawer 41 into air storage box 61, the movement of piston plate 63 sends the cold air stored between the two partitions 3 62 into the interior of connecting hole 68 through the gap between partition 3 62 and the inner side wall of storage cabinet 1. The cold air then flows back into storage drawer 41 through connecting hole 68, reusing the cold air and avoiding waste caused by the cold air spreading everywhere.

[0073] like Figures 4-11 As shown, in one embodiment, the storage drawer 41 is provided with a support assembly inside, the support assembly including a connecting plate 71 and a through hole 77;

[0074] There are multiple connecting plates 71, all of which slide through the side wall of the storage drawer 41 near the air storage box 61. Sliding plates 72 are slidably installed on the outer walls of the multiple connecting plates 71, and are fixedly installed on the inner bottom wall of the storage drawer 41. Both the connecting plates 71 and the sliding plates 72 are hollow, communicating with the cavity formed by the connecting plates 71 and the side walls of the two partitions 62. A receiving rod 73 is fixedly installed on the side wall of the sliding plate 72. A receiving groove 74 is formed on the top wall of the receiving rod 73. An extension rod 75 is slidably installed inside the receiving groove 74. A placement plate 8 is fixedly installed on the top wall of the extension rod 75. Multiple ventilation grooves are formed on the side wall of the placement plate 8. The embedding box is placed on top of the placement plate 8. The receiving rod 73 and the sliding plate... The side wall of 72 is provided with interconnected pressurizing holes 76. The bottom wall of the extension rod 75 is fixedly installed with a limiting strip 78. The bottom end of the side wall of the extension rod 75 is provided with a vent hole 79. The top end of the vent hole 79 passes through the side wall of the extension rod 75 and communicates with the outside. When the storage drawer 41 moves outward, the piston plate 63 shifts to one side of the storage drawer 41, thereby guiding the air on the rear side to the interior of the sliding plate 72 through the connecting plate 71, and entering the interior of the receiving rod 73 through the pressurizing holes 76. As a result, the air pressure inside the sliding plate 72 and the receiving rod 73 increases, causing the extension rod 75 to move upward due to the increase in bottom air pressure. Subsequently, the placement plate 8 and the embedding box on its top also move upward, making it convenient for the staff to take the embedding box out of the storage drawer 41 smoothly.

[0075] The sliding range of the extension rod 75 is limited by the limiting strip 78 to prevent the extension rod 75 from detaching from the inside of the receiving rod 73. Excess air inside the receiving rod 73 is discharged through the vent hole 79 to prevent the storage drawer 41 from being unable to move outward due to the limited air stored inside the sliding plate 72 and the air behind the piston plate 63 not being able to be discharged.

[0076] The through hole 77 is opened on the inner side wall of the sliding plate 72, and the through hole 77 is interconnected with multiple pressurization holes 76 inside the sliding plate 72. During the process of the storage drawer 41 moving outward, the connecting plate 71 gradually shifts away from the multiple pressurization holes 76 inside the sliding plate 72, and the multiple pressurization holes 76 are interconnected through the through hole 77, so that the air entering the interior of the sliding plate 72 can enter the interior of multiple receiving rods 73 simultaneously. This can avoid the situation where the pressurization holes 76 near the air storage box 61 cannot rise synchronously due to the lack of air entering because the connecting plate 71 is attached to the inner side wall of the sliding plate 72.

[0077] like Figures 4-11 As shown, in one embodiment, the storage drawer 41 is provided with a linkage component inside, which includes a positioning block 91 and a linkage bar 92;

[0078] The positioning blocks 91 are fixedly installed on the side wall of the communication pipe 67;

[0079] The linkage strip 92 is fixedly installed on the bottom wall surface of the positioning strip 46, the bottom wall surface of the linkage strip 92 and the top wall surface of the positioning block 91 are both fixedly installed with the magnetic block 93, the magnetic blocks 93 on the positioning block 91 and the linkage strip 92 are different in magnetic properties on the side wall surface close to each other; when the storage drawer 41 moves outward, since the communication pipe 67 is fixedly installed on the side wall of the gas storage tank 61, the position of the communication pipe 67 is unchanged, the positioning block 91 and the linkage strip 92 are connected together by the magnetic properties of the magnetic block 93, the position of the positioning strip 46 is unchanged, with the movement of the storage drawer 41, the folding plate 45 is folded, in the process of the storage drawer 41 being pulled out, the folding plate 45 is synchronously opened, further facilitating the worker to take out the embedding box, since the positioning block 91 and the linkage strip 92 are connected by the magnetic properties of the magnetic block 93, the worker can manually close or open the folding plate 45 by pulling the pull strip 47.

[0080] Specific working method is: through the air inlet hole 48, the cold air generated by the refrigeration device 31 is injected into the inside of the storage drawer 41 through the air pipe 32 and the air distribution hole 23, so that the inside of the storage drawer 41 is in a low temperature state, through the pull ring 42, the staff can pull out the storage drawer 41 from the top of the second partition plate 22, at this time, the pull rod 65 will pull the piston plate 63 to move together, because the side wall of the piston plate 63 is attached to the side wall of the two third partition plates 62 and the inside wall of the storage cabinet 1, so the piston plate 63 can suck the cold air in the storage drawer 41 into the cavity formed between the two third partition plates 62 through the communication hole 68, and store the cold air in the storage drawer 41; During the movement of the storage drawer 41, the piston plate 63 is offset to one side of the storage drawer 41, and then the air on the rear side is guided into the inside of the sliding plate 72 through the connecting plate 71, and enters the inside of the containing rod 73 through the pressurizing hole 76, so that the air pressure in the inside of the sliding plate 72 and the containing rod 73 is increased, so that the extension rod 75 moves upward due to the increase of the air pressure at the bottom, and then the placing plate 8 and the embedding box on the top thereof are also moved upward, so that the staff can smoothly take out the embedding box from the inside of the storage drawer 41; Because the connecting pipe 67 is fixedly installed on the side wall of the gas storage tank 61, the position of the connecting pipe 67 does not change during the movement of the storage drawer 41, the positioning block 91 and the linkage strip 92 are connected together by using the magnetism of the magnetic block 93, the position of the positioning strip 46 is fixed, and the folding plate 45 is folded with the movement of the storage drawer 41, during the movement of the storage drawer 41, the folding plate 45 is opened synchronously, further facilitating the staff to take out the embedding box; When the staff pushes the storage drawer 41 into the gas storage tank 61, the cold air stored between the two third partition plates 62 is sent into the inside of the communication hole 68 through the gap between the third partition plate 62 and the inside wall of the storage cabinet 1 by the movement of the piston plate 63, and is returned to the inside of the storage drawer 41 through the communication hole 68, so that the cold air is reused, and the waste caused by the diffusion of the cold air is avoided.

[0081] The technical means disclosed in the scheme of the application is not limited to the technical means disclosed in the above technical means, and also includes technical solutions composed of equivalent replacement of the above technical features. The unfinished matters of the application belong to the common knowledge of those skilled in the art.

Claims

1. A cryogenic storage device for mouse brain embedding cassettes used in neuroscience research, characterized by: The utility model provides a kind of storage cabinet, the inside of storage cabinet (1) is provided with separating component, separating component includes: Partition one (21), the partition one (21) is fixedly installed in the inside wall of storage cabinet (1) intermediate position, the left and right side wall of partition one (21) is equidistantly fixedly installed with multiple partition two (22), the side wall of multiple partition two (22) is fixedly connected with the inside wall of storage cabinet (1); Air holes (23), the air holes (23) are shared multiple, multiple air holes (23) are respectively opened in the inside wall of partition two (22), air holes (23) are located in the inside of partition two (22) close to the position of outside; The inside of storage cabinet (1) is provided with refrigeration component, refrigeration component includes: Refrigeration device (31), the refrigeration device (31) is fixedly installed on the inside bottom wall of storage cabinet (1); Vent pipe (32), the vent pipe (32) is opened in the inside of partition one (21), vent pipe (32) is communicated with multiple air holes (23), and the inside of vent pipe (32) is communicated with the output end of refrigeration device (31); The inside of storage cabinet (1) is provided with multiple placing components, and multiple placing components are respectively located at the top of multiple partition two (22), and placing component includes: Storage drawer (41), the storage drawer (41) is slidably installed on the top wall of partition two (22), and the front side wall of storage drawer (41) is fixedly installed with a pull ring (42), and the bottom wall of storage drawer (41) is provided with air inlet (48), and air inlet (48) is aligned with the air hole (23) of the adjacent air hole (23); The inside of storage cabinet (1) is provided with multiple gas storage components, and multiple gas storage components are respectively located at the rear side of multiple placing components, and the gas storage component includes: Gas storage tank (61), the gas storage tank (61) is fixedly installed on the top wall of partition two (22), and the inside of gas storage tank (61) is fixedly installed with two partition three (62) on the side wall close to storage drawer (41), the upper and lower side walls of two partition three (62) are fixedly connected with the inside upper and lower side walls of gas storage tank (61), and a certain gap is left between the inside side wall of gas storage tank (61) away from storage drawer (41) and two partition three (62), the inside of gas storage tank (61) is slidably installed with piston plate (63), the left and right side walls of piston plate (63) are close to the side wall of two partition three (62), the side wall of piston plate (63) is fixedly installed with pull rod (65), one end of pull rod (65) away from piston plate (63) is slidably penetrated through the side wall of gas storage tank (61) and is fixedly connected with storage drawer (41), the side wall of piston plate (63) is fixedly installed with sealing strip (64), sealing strip (64) is made of rubber, the side wall of pull rod (65) is sleeved with return spring (66), and the two ends of return spring (66) are respectively fixedly connected with the side wall of piston plate (63) and gas storage tank (61) corresponding. The communication pipe (67) is shared by multiple, multiple communication pipes (67) are respectively fixedly installed on the two sides of the side wall of the gas storage tank (61) near the storage drawer (41), the end of the communication pipe (67) away from the gas storage tank (61) is slidably penetrated through the side wall of the storage drawer (41), multiple communication pipes (67) are respectively aligned with the cavities formed by the two partition plates three (62) and the inner side wall of the storage cabinet (1), the side wall of the communication pipe (67) is provided with a communication hole (68), and the communication hole (68) is in communication with the interiors of the storage drawer (41) and the gas storage tank (61).

2. The low temperature storage device for a mouse brain embedding cassette used in neuroscience research according to claim 1, characterized in that: The placing assembly further comprises: The track strip (43) is fixedly installed at the top of the inner side wall of the storage drawer (41), the inner side wall of the track strip (43) is provided with a track groove (44), the side wall of the track groove (44) away from the inside is fixedly connected with a folding plate (45), the left and right ends of the folding plate (45) are slidably installed at the corresponding positions in the track groove (44), the inside of the track groove (44) is slidably installed with a positioning strip (46), the positioning strip (46) is fixedly connected with the corresponding side wall of the folding plate (45), and the top wall of the positioning strip (46) is fixedly installed with a tension strip (47).

3. The low temperature storage device for a mouse brain embedding cassette used in neuroscience research according to claim 1, characterized in that: The inside of the storage drawer (41) is provided with a supporting assembly, and the supporting assembly comprises: The communication plate (71) is shared by multiple, multiple communication plates (71) are slidably penetrated through the side wall of the storage drawer (41) near the gas storage tank (61), the outer side wall of the multiple communication plates (71) is slidably installed with a sliding plate (72), the sliding plate (72) is fixedly installed on the inner bottom wall of the storage drawer (41), the inside of the communication plate (71) and the sliding plate (72) is hollow, the cavity formed by the side wall of the communication plate (71) and the two partition plates three (62) is communicated, the side wall of the sliding plate (72) is fixedly installed with a containing rod (73), the top wall of the containing rod (73) is provided with a containing groove (74), the inside of the containing groove (74) is slidably installed with an extension rod (75), and the side wall of the containing rod (73) and the sliding plate (72) is provided with a plurality of pressure holes (76) in communication.

4. The low temperature storage device for a mouse brain embedding cassette used in neuroscience research according to claim 3, characterized in that: The top wall of the extension rod (75) is fixedly installed with a placing plate (8), a plurality of air grooves are formed in the side wall of the placing plate (8), and the embedding box is placed on the top of the placing plate (8).

5. The low temperature storage device for a mouse brain embedding cassette used in neuroscience research according to claim 3, characterized in that: The supporting assembly further comprises; The through hole (77) is formed in the inner side wall of the sliding plate (72), and the through hole (77) is in communication with the multiple pressure holes (76) in the sliding plate (72).

6. The low temperature storage device for a mouse brain embedding cassette used in neuroscience research according to claim 3, characterized in that: The bottom wall of the extension rod (75) is fixedly installed with a limiting strip (78), the bottom end of the side wall of the extension rod (75) is provided with a ventilation hole (79), and the top end of the ventilation hole (79) penetrates through the side wall of the extension rod (75) and communicates with the outside.

7. The low temperature storage device for a mouse brain embedding cassette used in neuroscience research according to claim 2, characterized in that: The inside of the storage drawer (41) is provided with a linkage assembly, and the linkage assembly comprises: The positioning block (91) is shared by multiple, multiple positioning blocks (91) are fixedly installed on the side wall of the communication pipe (67); Linkage strip (92), the linkage strip (92) is fixedly installed on the bottom wall surface of the positioning strip (46), the bottom wall surface of the linkage strip (92) and the top wall surface of the positioning block (91) are fixedly installed with magnetic blocks (93), the magnetic blocks (93) on the positioning block (91) and the magnetic blocks (93) on the linkage strip (92) are different in magnetic properties on the side wall surface close to each other.

8. A mouse brain embedding cassette for neuroscience research, stored using the cryogenic storage device of any one of claims 1-7, wherein: Include: Storage plate (51), the top wall surface of the storage plate (51) is provided with a storage groove (52); Cover plate (53), the cover plate (53) covers the top of the storage plate (51), the top wall surface of the cover plate (53) is provided with an indicating groove (54), and the indicating groove (54) is a trapezoidal groove.

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