Feed additive composition as well as preparation method and application thereof

Abstract

The invention discloses a feed additive composition as well as a preparation method and application thereof, and belongs to the technical field of sturgeon culture. The feed additive composition comprises arachidonic acid, vitamin C and pollen. Besides, the invention also provides a preparation method of the feed additive composition, which comprises the following steps: mixing the selenium yeast, the first starch and the first water, drying to obtain first particles, and mixing the first particles, the arachidonic acid, the vitamin C and the pollen to obtain the feed additive composition. In addition, the invention further provides application of the feed additive composition in sturgeon feed. According to the feed additive composition provided by the invention, the immunity of sturgeons can be effectively improved under the cooperation of the arachidonic acid, the vitamin C and the pollen.

Description

Technical Field

[0001] This invention relates to the field of sturgeon farming technology, specifically to a feed additive composition, its preparation method, and its application. Background Technology

[0002] Sturgeon are among the earliest groups of vertebrates, with fossils of acipenseriformes discovered as early as the Jurassic period of the Mesozoic Era, 200 million years ago. Acipenseriformes are characterized by their large size, long lifespan, and late sexual maturity. There are 27 species of sturgeon worldwide, but due to factors such as water conservancy projects, fishing, shipping, and pollution, most are currently endangered to varying degrees. my country has eight species of sturgeon: the Daurian sturgeon (Husodauricus) and the Shih-renckr sturgeon (Acipenser schrenckr) in the Heilongjiang River; the Siberian sturgeon (Acipenser baer), the small sturgeon (Acipenser ruthanus), and the naked sturgeon (Acipenser nudiventris) in the Ertis River and other waters of Xinjiang; the Chinese sturgeon (Acipenser sinensis Gray), which migrates between rivers and the sea; the Yangtze sturgeon (Acipenser dabryanus), which lives in freshwater; and the Chinese paddlefish (Pesphurus gladius), which inhabits the estuaries of the Yangtze River and other major rivers. Unfortunately, the Chinese paddlefish, known as the "King of Freshwater Fish in China," was declared functionally extinct in 2020. On July 21, 2022, the International Union for Conservation of Nature (IUCN) declared the Yangtze sturgeon "extinct in the wild." The Chinese sturgeon is also facing the dilemma of continuous interruption of natural reproduction (2017-2024). If active and effective artificial protection measures are not taken, more and more sturgeon populations may disappear from our sight in the future.

[0003] The Yangtze sturgeon is a freshwater, sedentary sturgeon species found in the upper and middle reaches of the Yangtze River in my country. It has a long sexual maturity cycle, with females reaching sexual maturity at 6-8 years of age and males at 3-5 years of age. After the 1980s, its natural population shrank dramatically, and its natural reproductive activity ceased after 2000. It was listed as a Class I protected wild animal in China in 1988, listed in Appendix II of the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) in 1997, and upgraded to Critically Endangered (CR) by the International Union for Conservation of Nature (IUCN) in 2010. In 2022, it was declared "Extinct in the Wild" by the IUCN.

[0004] In recent years, with the development of artificial breeding technology, large-scale farming of Yangtze sturgeon has gradually emerged. However, because the immune function of juvenile fish is not yet fully developed, they are more sensitive to fluctuations in water temperature, changes in water quality, and potential pathogens in the breeding environment. This makes them prone to problems such as slow growth, low survival rate, and weak disease resistance, which has become a key bottleneck restricting the efficient development of the sturgeon farming industry.

[0005] Feed is a core factor affecting the growth and immune performance of aquatic animals, and its formulation directly determines the profitability of aquaculture. Currently, commercially available sturgeon fry feeds mostly aim to meet basic nutritional needs, primarily by controlling the content of conventional nutrients such as crude protein and crude fat to ensure the growth of fry. However, there is a lack of functional design specifically for "enhancing immune performance." To address this issue, the industry has begun to experiment with adding functional additives to feed, but these have not significantly improved the immunity of sturgeon. Summary of the Invention

[0006] The purpose of this invention is to overcome the above-mentioned technical deficiencies and provide a feed additive composition, its preparation method and application, thereby solving the technical problem of how to significantly improve the immunity of sturgeon in the prior art.

[0007] To achieve the above-mentioned technical objectives, the present invention provides a feed additive composition comprising arachidonic acid, vitamin C, and pollen.

[0008] In any embodiment, the amount of arachidonic acid added is 1-1.5% of the feed mass; the amount of vitamin C added is 0.01%-0.08% of the feed mass; and the amount of pollen added is 0.15%-0.9% of the feed mass.

[0009] In any embodiment, the amount of arachidonic acid added is 1% of the feed mass; the amount of vitamin C added is 0.04% of the feed mass; and the amount of pollen added is 0.45% of the feed mass.

[0010] Alternatively, the amount of arachidonic acid added is 1% of the feed mass; the amount of vitamin C added is 0.08% of the feed mass; and the amount of pollen added is 0.9% of the feed mass.

[0011] In any embodiment, it further includes yeast selenium and a first starch, wherein the amount of yeast selenium added is 0.2-0.5% of the feed mass; and the amount of the first starch added is 10%-20% of the yeast selenium mass.

[0012] In any embodiment, it further includes β-glucan, a second starch, and gum arabic, wherein the amount of β-glucan added is 0.3-0.6% of the feed mass, the amount of the second starch added is 10%-20% of the β-glucan mass, and the amount of gum arabic added is 5-10% of the β-glucan mass.

[0013] In any embodiment, the feed, calculated by weight, includes 15-20 parts fish meal, 20-30 parts soybean meal, 10-15 parts chicken meal, 10-15 parts fermented meal, 14-16 parts wheat flour, 1-2 parts phospholipids, 2-4 parts calcium dihydrogen phosphate, 2-4 parts compound multivitamins and minerals, and 5-10 parts oil.

[0014] In addition, the present invention also proposes a method for preparing a feed additive composition, comprising the following steps: mixing yeast selenium, first starch and first water and drying to obtain first granules, and mixing the first granules, arachidonic acid, vitamin C and pollen to obtain the feed additive composition.

[0015] In any embodiment, the method further includes: mixing the first particle, β-glucan, second starch, gum arabic, and second water, and then drying the mixture to obtain a second particle; and mixing the second particle, arachidonic acid, vitamin C, and pollen to obtain the feed additive composition.

[0016] Furthermore, the present invention also proposes the application of the above-mentioned feed additive composition in sturgeon feed.

[0017] In any embodiment, the steps include: mixing the feed additive composition with sturgeon feed, followed by adding a binder and a third water and mixing.

[0018] Compared with the prior art, the beneficial effects of the present invention include: the feed additive composition proposed in the present invention, in combination with arachidonic acid, vitamin C and pollen, can effectively improve the immunity of sturgeon. Attached Figure Description

[0019] Figure 1 This is a photograph of a sturgeon used in an application example of the present invention. Detailed Implementation

[0020] The "range" disclosed in this application is defined by a lower limit and an upper limit. A given range is defined by selecting a lower limit and an upper limit, which define the boundaries of a particular range. Ranges defined in this way can include or exclude endpoints and can be arbitrarily combined; that is, any lower limit can be combined with any upper limit to form a range. For example, if ranges of 60–120 and 80–110 are listed for a specific parameter, it is understood that ranges of 60–110 and 80–120 are also expected. Furthermore, if minimum range values ​​of 1 and 2 are listed, and if maximum range values ​​of 3, 4, and 5 are listed, then the following ranges are all expected: 1–3, 1–4, 1–5, 2–3, 2–4, and 2–5. In this application, unless otherwise stated, the numerical range "a–b" represents a shortened representation of any combination of real numbers between a and b, where a and b are real numbers. For example, the numerical range "0~5" indicates that all real numbers between "0~5" have been listed in this article; "0~5" is simply a shortened representation of these numerical combinations. Furthermore, when a parameter is stated as an integer ≥2, it is equivalent to disclosing that the parameter is, for example, an integer such as 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, etc.

[0021] Unless otherwise specified, the terms "comprising" and "including" as used in this application can be open-ended or closed-ended. For example, "comprising" and "including" can mean that other components not listed may also be included, or that only the listed components may be included.

[0022] Unless otherwise specified, the term "or" is inclusive in this application. For example, the phrase "A or B" means "A, B, or both A and B". More specifically, the condition "A or B" is satisfied by any of the following conditions: A is true (or exists) and B is false (or does not exist); A is false (or does not exist) and B is true (or exists); or both A and B are true (or exist).

[0023] This specific embodiment provides a feed additive composition comprising arachidonic acid, vitamin C, and pollen; the amount of arachidonic acid added is 1-1.5% of the feed mass; the amount of vitamin C added is 0.01%-0.08% of the feed mass; and the amount of pollen added is 0.15%-0.9% of the feed mass.

[0024] In some embodiments, the amount of arachidonic acid added is 1% of the feed mass; the amount of vitamin C added is 0.04% of the feed mass; and the amount of pollen added is 0.45% of the feed mass.

[0025] Alternatively, the amount of arachidonic acid added is 1% of the feed mass; the amount of vitamin C added is 0.08% of the feed mass; and the amount of pollen added is 0.9% of the feed mass.

[0026] In some embodiments, the feed also includes selenium yeast and a first starch, wherein the amount of selenium yeast added is 0.2-0.5% of the feed mass; and the amount of the first starch added is 10%-20% of the selenium yeast mass. To further improve the immunity of sturgeon, we added selenium yeast and, in combination with the first starch, formed first pellets which were added to the feed; however, the effect on improving the immunity of sturgeon was not significant.

[0027] Based on this, we further added β-glucan and, through combination with secondary starch and gum arabic, formulated a second granule capable of slow-release of the active ingredient. With the synergistic effect of β-glucan, yeast selenium, and other components, this significantly improved the immunity of sturgeon. This is likely because the slow-release β-glucan enhances the integrity of the sturgeon's intestinal mucosa, improving the absorption of other active ingredients in the feed, thereby effectively enhancing the sturgeon's immunity.

[0028] In some embodiments, the feed may further include β-glucan, a second starch, and gum arabic, wherein the amount of β-glucan added is 0.3-0.6% of the feed mass, the amount of the second starch added is 10%-20% of the β-glucan mass, and the amount of gum arabic added is 5-10% of the β-glucan mass.

[0029] Furthermore, this specific embodiment also proposes a method for preparing a feed additive composition, including the following steps: mixing yeast selenium, first starch and first water and drying to obtain first granules, and mixing the first granules, arachidonic acid, vitamin C and pollen to obtain the feed additive composition.

[0030] In some embodiments, the method further includes: mixing the first particle, β-glucan, second starch, gum arabic, and second water, and then drying the mixture to obtain a second particle; and mixing the second particle, arachidonic acid, vitamin C, and pollen to obtain the feed additive composition.

[0031] This specific embodiment also proposes the application of feed additive compositions in sturgeon feed.

[0032] In some embodiments, the above application includes the following steps: mixing the feed additive composition with sturgeon feed, followed by adding a binder and a third water mixture.

[0033] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the invention.

[0034] In this invention, the terms "some embodiments," "this embodiment," and examples are used to describe a subset of all possible embodiments. However, it is understood that "some embodiments" can be the same subset or different subsets of all possible embodiments and can be combined with each other without conflict.

[0035] If the application documents contain similar descriptions such as "first / second", the following explanation shall be added: In the following description, the terms "first / second / third" are used only to distinguish similar objects and do not represent a specific ordering of objects. It is understood that "first / second / third" may be interchanged in a specific order or sequence where permitted, so that the embodiments described herein can be implemented in an order other than that illustrated or described herein.

[0036] In this embodiment, the term "and / or" is merely a description of the relationship between related objects, indicating that there can be three relationships. For example, object A and / or object B can represent three situations: object A exists alone, object A and object B exist simultaneously, and object B exists alone.

[0037] The following describes embodiments of this application. The embodiments described below are exemplary and are only used to explain this application, and should not be construed as limiting this application. Where specific techniques or conditions are not specified in the embodiments, they are performed according to the techniques or conditions described in the literature in this field or according to the product instructions. Reagents or instruments used, unless otherwise specified, are all conventional products that can be obtained commercially.

[0038] The binder used in the following examples or comparative examples was purchased from Inner Mongolia Hezhengmei Biotechnology Co., Ltd., and the amount used was 0.5% of the weight of the sturgeon feed. The amount of the third water added in the following examples or comparative examples was 10% of the weight of the sturgeon feed.

[0039] The composite multivitamin and mineral raw materials used in the following examples or comparative examples are sourced from existing technologies. The composite multivitamin and mineral raw material composition includes vitamin A acetate, vitamin D3, d1-α-tocopherol acetate, vitamin K3, vitamin B2, vitamin B6, and vitamin B1. 12The compound contains L-ascorbic acid 2-phosphate, D-calcium pantothenate, nicotinamide, folic acid, D-biotin, inositol, zinc sulfate, copper sulfate, ferrous sulfate, cobalt phosphate, calcium iodate, and sodium selenite; the carrier is defatted rice bran and dizeolite powder; the content of each component per kilogram of the compound multivitamin and mineral is as follows: Vitamin A ≥ 450,000 IU, folic acid ≥ 0.3 g, Vitamin D3 200,000-240,000 IU, D-biotin ≥ 10 mg, d1-α-tocopheryl acetate ≥ 8.0 g, inositol ≥ 12 g, Vitamin K3 ≥ 0.6 g, zinc 2.0-6.0 g, Vitamin B1 ≥ 1.0 g, manganese 1.0-5.0 g, Vitamin B2 ≥ 1.2 g, copper 0.75-1.5 g, Vitamin B6 ≥ 1.0 g, iron 6.0-15 g, and vitamin B... 12 ≥5.0 mg, Cobalt 0.15-0.18 g, L-ascorbic acid 2-phosphate ≥42 g, Iodine 0.01-1.0 g, D-calcium pantothenate ≥2.4 g, Selenium 0.02-0.04 g, Nicotinamide ≥10 g, Moisture ≤10%.

[0040] In the following embodiments, the first particle has a mesh size of 110-120, and the second particle has a mesh size of 80-90.

[0041] Example 1

[0042] This embodiment proposes a feed additive composition comprising arachidonic acid, vitamin C, and pollen; the amount of arachidonic acid added is 1% of the feed mass; the amount of vitamin C added is 0.01% of the feed mass; and the amount of pollen added is 0.15% of the feed mass.

[0043] The method for preparing the feed additive composition of this embodiment includes the following steps: mixing arachidonic acid, vitamin C and pollen to obtain the feed additive composition.

[0044] The feed (i.e. sturgeon feed) in this embodiment, calculated by weight, includes 18 parts fish meal, 25 parts soybean meal, 12 parts chicken meal, 15 parts fermented meal, 15 parts wheat flour, 1 part phospholipid, 2 parts calcium dihydrogen phosphate, 4 parts compound multivitamins and minerals, and 8 parts oil.

[0045] This embodiment also proposes the application of the feed additive composition in sturgeon feed, including the following steps: mixing the feed additive composition with sturgeon feed evenly, and then adding a binder and a third water and mixing evenly.

[0046] Example 2

[0047] This embodiment proposes a feed additive composition comprising arachidonic acid, vitamin C, and pollen; the amount of arachidonic acid added is 1% of the feed mass; the amount of vitamin C added is 0.04% of the feed mass; and the amount of pollen added is 0.45% of the feed mass.

[0048] The method for preparing the feed additive composition of this embodiment includes the following steps: mixing arachidonic acid, vitamin C and pollen to obtain the feed additive composition.

[0049] The feed (i.e. sturgeon feed) in this embodiment, calculated by weight, includes 15 parts fish meal, 30 parts soybean meal, 10 parts chicken meal, 10 parts fermented meal, 14 parts wheat flour, 2 parts phospholipids, 3 parts calcium dihydrogen phosphate, 3 parts compound multivitamins and minerals, and 10 parts oil.

[0050] This embodiment also proposes the application of the feed additive composition in sturgeon feed, including the following steps: mixing the feed additive composition with sturgeon feed evenly, and then adding a binder and a third water and mixing evenly.

[0051] Example 3

[0052] This embodiment proposes a feed additive composition comprising arachidonic acid, vitamin C, and pollen; the amount of arachidonic acid added is 1% of the feed mass; the amount of vitamin C added is 0.08% of the feed mass; and the amount of pollen added is 0.9% of the feed mass.

[0053] The method for preparing the feed additive composition of this embodiment includes the following steps: mixing arachidonic acid, vitamin C and pollen to obtain the feed additive composition.

[0054] The feed (i.e. sturgeon feed) in this embodiment, calculated by weight, includes 20 parts fish meal, 20 parts soybean meal, 15 parts chicken meal, 12 parts fermented meal, 16 parts wheat flour, 1.5 parts phospholipids, 4 parts calcium dihydrogen phosphate, 2 parts compound multivitamins and minerals, and 5 parts oil.

[0055] This embodiment also proposes the application of the feed additive composition in sturgeon feed, including the following steps: mixing the feed additive composition with sturgeon feed evenly, and then adding a binder and a third water and mixing evenly.

[0056] Example 4

[0057] This embodiment proposes a feed additive composition comprising arachidonic acid, vitamin C, and pollen; the amount of arachidonic acid added is 1% of the feed mass; the amount of vitamin C added is 0.04% of the feed mass; the amount of pollen added is 0.45% of the feed mass; it also includes yeast selenium and a first starch, the amount of yeast selenium added is 0.3% of the feed mass; the amount of the first starch added is 15% of the yeast selenium mass.

[0058] This embodiment also proposes a method for preparing a feed additive composition, including the following steps: mixing yeast selenium, first starch and first water and drying to obtain first granules, mixing the first granules, arachidonic acid, vitamin C and pollen to obtain the feed additive composition, wherein the amount of first water added is 10% of the first starch.

[0059] This embodiment also proposes the application of the feed additive composition in sturgeon feed, including the following steps: mixing the feed additive composition with sturgeon feed evenly, and then adding a binder and a third water and mixing evenly.

[0060] Example 5

[0061] This embodiment proposes a feed additive composition comprising arachidonic acid, vitamin C, and pollen; the amount of arachidonic acid added is 1% of the feed mass; the amount of vitamin C added is 0.04% of the feed mass; the amount of pollen added is 0.45% of the feed mass; it also includes yeast selenium and a first starch, the amount of yeast selenium added is 0.5% of the feed mass; the amount of the first starch added is 10% of the yeast selenium mass.

[0062] This embodiment also proposes a method for preparing a feed additive composition, including the following steps: mixing yeast selenium, first starch and first water and drying to obtain first granules, mixing the first granules, arachidonic acid, vitamin C and pollen to obtain the feed additive composition, wherein the amount of first water added is 10% of the first starch.

[0063] This embodiment also proposes the application of the feed additive composition in sturgeon feed, including the following steps: mixing the feed additive composition with sturgeon feed evenly, and then adding a binder and a third water and mixing evenly.

[0064] Example 6

[0065] This embodiment proposes a feed additive composition comprising arachidonic acid, vitamin C, and pollen; the amount of arachidonic acid added is 1% of the feed mass; the amount of vitamin C added is 0.04% of the feed mass; the amount of pollen added is 0.45% of the feed mass; it also includes yeast selenium and a first starch, the amount of yeast selenium added is 0.3% of the feed mass; the amount of the first starch added is 15% of the yeast selenium mass; it further includes β-glucan, a second starch, and gum arabic, the amount of β-glucan added is 0.4% of the feed mass, the amount of the second starch added is 15% of the β-glucan mass, and the amount of gum arabic added is 8% of the β-glucan mass.

[0066] This embodiment also proposes a method for preparing a feed additive composition, comprising the following steps: mixing yeast selenium, a first starch and a first water and drying to obtain a first particle; mixing the first particle, β-glucan, a second starch, gum arabic and a second water and drying to obtain a second particle; mixing the second particle, arachidonic acid, vitamin C and pollen to obtain the feed additive composition, wherein the amount of the first water added is 10% of the first starch and the amount of the second water added is 12% of the second starch.

[0067] This embodiment also proposes the application of the feed additive composition in sturgeon feed, including the following steps: mixing the feed additive composition with sturgeon feed evenly, and then adding a binder and a third water and mixing evenly.

[0068] Comparative Example 1

[0069] The difference between this comparative example and Example 2 is that the feed additive in this comparative example is arachidonic acid; the amount of arachidonic acid added is 1% of the feed mass.

[0070] This embodiment also proposes the application of feed additives in sturgeon feed, including the following steps: mixing arachidonic acid and sturgeon feed evenly, and then adding binder and first water and mixing evenly.

[0071] Comparative Example 2

[0072] The difference between this comparative example and Example 2 is that the feed additive composition in this comparative example is arachidonic acid and vitamin C; the amount of arachidonic acid added is 1% of the feed mass, and the amount of vitamin C added is 0.04% of the feed mass.

[0073] This embodiment also proposes the application of feed additives in sturgeon feed, including the following steps: arachidonic acid and vitamin C are mixed evenly, then sturgeon feed is added and mixed evenly, then binder and first water are added and mixed evenly.

[0074] Comparative Example 3

[0075] The difference between this comparative example and Example 2 is that the feed additives in this comparative example are arachidonic acid and pollen; the amount of arachidonic acid added is 1% of the feed mass, and the amount of pollen added is 0.45% of the feed mass.

[0076] This embodiment also proposes the application of feed additives in sturgeon feed, including the following steps: mixing arachidonic acid and pollen, then adding sturgeon feed and mixing evenly, then adding binder and first water and mixing evenly.

[0077] Comparative Example 4

[0078] The components and dosages of this comparative feed additive composition are the same as those in Example 6.

[0079] This comparative example also proposes a method for preparing a feed additive composition, comprising the following steps: mixing yeast selenium, first starch, β-glucan, second starch, gum arabic, second water, and third water evenly and then drying to obtain first granules; mixing the first granules, arachidonic acid, vitamin C, and pollen evenly to obtain the feed additive composition.

[0080] This comparative example also proposes the application of the feed additive composition in sturgeon feed, including the following steps: mixing the feed additive composition with sturgeon feed evenly, and then adding a binder and a third water and mixing evenly.

[0081] Comparative Example 5

[0082] The feed additive composition of this comparative example comprises yeast selenium and a first starch, wherein the amount of yeast selenium added is 0.3% of the feed mass; the amount of the first starch added is 15% of the yeast selenium mass; it also comprises β-glucan, a second starch and gum arabic, wherein the amount of β-glucan added is 0.4% of the feed mass, the amount of the second starch added is 15% of the β-glucan mass, and the amount of gum arabic added is 8% of the β-glucan mass.

[0083] This comparative example also proposes a method for preparing a feed additive composition, comprising the following steps: mixing yeast selenium, a first starch and a first water and drying to obtain a first particle; mixing the first particle, β-glucan, a second starch, gum arabic and a second water and drying to obtain a second particle; the second particle is the feed additive composition; the amount of the first water added is 10% of the first starch and the amount of the second water added is 12% of the second starch.

[0084] This comparative example also proposes the application of the feed additive composition in sturgeon feed, including the following steps: mixing the second pellet with sturgeon feed evenly, and then adding a binder and a third water and mixing evenly.

[0085] Feeding experiment

[0086] Before the experiment, 1100 healthy, uniformly sized juvenile Yangtze sturgeon were selected and reared in 11 indoor circular flow-through culture ponds. Each pond had a diameter of 3 meters, a depth of 0.75 meters, and an effective culture volume of 5.29 cubic meters. After two weeks of acclimatization to commercial feed, the juvenile Yangtze sturgeon with an initial average weight of (190.13 ± 38.18 g) were randomly reared in the 11 indoor circular flow-through culture ponds for an 8-week period. During the experiment, apparent satiety was used for feeding, twice daily, at 8:00 AM and 8:00 PM, using feeds containing feed additive compositions as described in Examples 1-6 and Comparative Examples 1-5, at approximately 1% of the fish's body weight. Wastewater was drained twice daily, each time draining 30% of the culture pond volume. During the aquaculture period, the water temperature should be 18.8–20.4℃, dissolved oxygen ≥5.5mg / L, pH 7.98–8.05, and ammonia nitrogen <0.05mg / L. Figure 1 Photos taken during the feeding of sturgeon.

[0087] Comparative Examples 1-3 correspond to F1-F3, Examples 1-3 correspond to F4-F6, Examples 4-6 correspond to F7-F9, and Comparative Example 4-5 corresponds to F10-F11. The detected performance parameters are shown in Tables 1-8. It should be noted that n in Tables 1 and 2 represents the total number of fish, and the measured performance parameters are the total performance parameters of n fish.

[0088] Table 1. Effects of the feed additive compositions of Examples 1-3 and Comparative Examples 1-3 on growth performance, feed utilization, and survival rate of juvenile Yangtze sturgeon.

[0089]

[0090] Table 2. Effects of the feed additive compositions of Examples 4-6 and Comparative Examples 4-5 on the growth performance and survival rate of juvenile Yangtze sturgeon.

[0091]

[0092] Table 3. Effects of feed additive compositions from Examples 1-3 and Comparative Examples 1-3 on blood health indicators of juvenile Yangtze sturgeon.

[0093]

[0094] Table 4. Effects of the feed additive compositions of Examples 4-6 and Comparative Examples 4-5 on blood health indicators of juvenile Yangtze sturgeon.

[0095]

[0096] Table 5. Effects of the feed additive compositions of Examples 1-3 and Comparative Examples 1-3 on the antioxidant capacity of the liver of juvenile Yangtze sturgeon.

[0097]

[0098] Table 6. Effects of the feed additive compositions of Examples 4-6 and Comparative Examples 4-5 on the antioxidant capacity of the liver of juvenile Yangtze sturgeon.

[0099]

[0100] Table 7. Effects of the feed additive compositions of Examples 1-3 and Comparative Examples 1-3 on the spleen immune function of juvenile Yangtze sturgeon.

[0101]

[0102] Table 8. Effects of the feed additive compositions of Examples 4-6 and Comparative Examples 4-5 on the spleen immune function of juvenile Yangtze sturgeon.

[0103]

[0104] As can be seen from Tables 1-8, the feed additive composition of Example 2 can significantly improve the antioxidant capacity of juvenile fish livers. The feed additive compositions of Examples 4-5 are comparable to Example 2 in improving the antioxidant capacity of juvenile fish livers. The feed additive composition of Example 6 is the most significant in improving the antioxidant capacity of juvenile fish livers. In addition, the feed additive composition of Example 2 can significantly improve the spleen immune performance of juvenile Yangtze sturgeon. The feed additives of Examples 4-5 are comparable to Example 2 in improving the spleen immune performance of juvenile Yangtze sturgeon. The feed additive composition of Example 6 is the most significant in improving the spleen immune performance of juvenile Yangtze sturgeon.

[0105] The specific embodiments of the present invention described above do not constitute a limitation on the scope of protection of the present invention. Any other corresponding changes and modifications made in accordance with the technical concept of the present invention should be included within the scope of protection of the claims of the present invention.

Claims

1. A feed additive composition, characterized in that, It includes arachidonic acid, vitamin C, and pollen.

2. The feed additive composition according to claim 1, characterized in that, The amount of arachidonic acid added is 1-1.5% of the feed weight; the amount of vitamin C added is 0.01%-0.08% of the feed weight; and the amount of pollen added is 0.15%-0.9% of the feed weight.

3. The feed additive composition according to claim 2, characterized in that, The amount of arachidonic acid added is 1% of the feed weight; the amount of vitamin C added is 0.04% of the feed weight; and the amount of pollen added is 0.45% of the feed weight. Alternatively, the amount of arachidonic acid added is 1% of the feed mass; the amount of vitamin C added is 0.08% of the feed mass; and the amount of pollen added is 0.9% of the feed mass.

4. The feed additive composition according to claim 2, characterized in that, It also includes yeast selenium and a first starch, wherein the amount of yeast selenium added is 0.2-0.5% of the feed mass; and the amount of the first starch added is 10%-20% of the yeast selenium mass.

5. The feed additive composition according to claim 4, characterized in that, It also includes β-glucan, secondary starch and gum arabic, wherein the amount of β-glucan added is 0.3-0.6% of the feed mass, the amount of secondary starch added is 10%-20% of the β-glucan mass, and the amount of gum arabic added is 5-10% of the β-glucan mass.

6. The feed additive composition according to any one of claims 1-5, characterized in that, The feed is calculated by weight, including 15-20 parts fish meal, 20-30 parts soybean meal, 10-15 parts chicken meal, 10-15 parts fermented meal, 14-16 parts wheat flour, 1-2 parts phospholipids, 2-4 parts calcium dihydrogen phosphate, 2-4 parts compound multivitamins and minerals, and 5-10 parts oil.

7. A method for preparing a feed additive composition, characterized in that, The process includes the following steps: mixing yeast selenium, first starch and first water and drying to obtain first granules; mixing the first granules, arachidonic acid, vitamin C and pollen to obtain the feed additive composition.

8. The method for preparing the feed additive composition according to claim 7, characterized in that, Also includes: The first particle, β-glucan, second starch, gum arabic, and second water are mixed and dried to obtain the second particle. The second particle, arachidonic acid, vitamin C, and pollen are mixed to obtain the feed additive composition.

9. The use of the feed additive composition according to any one of claims 1-6 in sturgeon feed.

10. The application according to claim 9, characterized in that, Includes the following steps: The feed additive composition is mixed with sturgeon feed, and then a binder and a third water are added and mixed.

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