Compound turpentine liniment as well as preparation method and application thereof

Abstract

The invention relates to a compound turpentine liniment as well as a preparation method and application of the compound turpentine liniment. Comprising the following preparation raw materials in percentage by mass: 8 to 12 percent of turpentine, 45 to 55 percent of glycerol, 12 to 18 percent of cyperus esculentus extract, 5 to 10 percent of soft soap, 0.5 to 1.5 percent of camphor, 0.1 to 1 percent of glucosamine, 0.5 to 2 percent of chondroitin sulfate, 0.1 to 1 percent of dimethyl sulfone, 0.5 to 2.5 percent of oil-soluble azone, 0.1 to 1 percent of lauramide propyl amine oxide and 12 to 20 percent of purified water. The cyperus esculentus extract prepared by the preparation method disclosed by the invention is compounded with the turpentine for use, a certain synergistic effect exists between the cyperus esculentus extract and the turpentine, the effect of reducing inflammatory factors in serum of the compound turpentine liniment can be improved, and the high-temperature-resistant stability is good; the compound turpentine liniment has an obvious better effect on reducing inflammatory factors in serum, so that a synergistic effect possibly exists between cellulase and mannase, and the compound turpentine liniment has a better curative effect on arthritis.

Description

Technical Field

[0001] This invention belongs to the field of pet medicine technology, specifically relating to a compound turpentine oil liniment, its preparation method, and its application. Background Technology

[0002] Arthritis is a common chronic inflammatory disease that primarily affects the joints of pet cats and dogs. The main types include osteoarthritis (also known as degenerative joint disease, a chronic, progressive wear and tear and degeneration of articular cartilage, most common in older pets), immune-mediated arthritis (such as rheumatoid arthritis), infectious arthritis (caused by bacterial, viral, or fungal infections, usually accompanied by fever and systemic symptoms), and traumatic arthritis (caused by joint injuries such as fractures, ligament tears, or joint dislocations). Common symptoms include lameness, joint swelling, limited mobility, pain, muscle atrophy, and behavioral changes. Furthermore, certain breeds of dogs (such as Labradors, Golden Retrievers, and German Shepherds) are more prone to arthritis due to developmental issues such as hip dysplasia, elbow dysplasia, and patellar dislocation.

[0003] Currently, chemical drugs are used to treat arthritis in pets, such as meloxicam, carbofen, and feloxibuxo. However, arthritis treatment is a long-term process that requires long-term medication. Single oral medications are slow to reduce swelling and pain and are prone to causing drug resistance. Therefore, this invention provides a compound turpentine oil liniment with natural ingredients and its preparation method. Summary of the Invention

[0004] The purpose of this invention is to solve the above-mentioned problems and provide a compound turpentine oil liniment, its preparation method, and its application.

[0005] The present invention achieves the above objectives through the following technical solutions: This invention provides a compound turpentine oil liniment, comprising the following raw materials in weight percentages: turpentine oil 8-12%, glycerin 45-55%, tiger pea extract 12-18%, soft soap 5-10%, camphor 0.5-1.5%, glucosamine 0.1-1%, chondroitin sulfate 0.5-2%, dimethyl sulfone 0.1-1%, oil-soluble azone 0.5-2.5%, laurylamidopropylamine oxide 0.1-1%, and purified water 12-20%.

[0006] As a further optimization of the present invention, the compound turpentine liniment comprises the following raw materials in weight percentage: 10% turpentine oil, 43.9% glycerin, 15% tiger pea extract, 7.5% soft soap, 1.0% camphor, 0.5% glucosamine, 1% chondroitin sulfate, 0.3% dimethyl sulfone, 2% oil-soluble azone, 0.3% lauramidopropylamine oxide, and 18.5% purified water.

[0007] As a further optimization of the present invention, the method for obtaining the tiger nut extract is as follows: tiger nut granules that have been screened, washed and dried are soaked in purified water, the pH is adjusted to 5.0-6.0, and after soaking at 40-50℃ for 1-2 hours, ultrasonic-assisted extraction is performed. The resulting extract is concentrated, freeze-dried and pulverized to obtain a powdered tiger nut extract.

[0008] As a further optimization of the present invention, the power of the ultrasound-assisted extraction is 300-500W, the frequency is 35-60kHz, the temperature is 50℃-60℃, and the time is 30-40min. Specifically, the ultrasound-assisted extraction involves ultrasound treatment for 10s, followed by a 5s pause, and this cycle is repeated.

[0009] As a further optimization of the present invention, when the tiger nuts are soaked, an enzymatic hydrolysant is added to the purified water. The amount of enzymatic hydrolysant used per 1 kg of tiger nuts is 0.1-0.5 g. The enzymatic hydrolysant includes at least one of cellulase and mannanase. When an enzymatic hydrolysate is added to the purified water, it should be inactivated at 90-100℃ for 10-20 minutes after soaking.

[0010] A method for preparing a compound turpentine oil liniment, the specific steps of which are as follows: (1) The soft soap, camphor powder, turpentine oil, glycerin and tiger pea extract were first ground in a colloid mill to obtain a preliminary mixed emulsion; then glucosamine and chondroitin sulfate were added for a second grinding; then dimethyl sulfone, oil-soluble azone and laurylamidopropylamine oxide were added for a third grinding to obtain a mixture; (2) Transfer the mixture to a glass bottle, add purified water, and shake to emulsify it completely to obtain compound turpentine liniment.

[0011] As a further optimization of the present invention, in step (1), the first grinding is carried out at 30-35℃ and 300-400r / min for 1-2 hours; The second grinding is carried out at 30-35℃ and 200-300r / min for 10-20min. The third grinding is carried out at 25-30℃ and 350-450r / min for 20-35 minutes.

[0012] As a further optimization of the present invention, the shaking treatment is a shaking treatment at 60r / min for 20-30min in a constant temperature water bath shaker at 35-45℃.

[0013] The use of a compound turpentine oil liniment in the preparation of a medicine for treating or improving arthritis in pets.

[0014] As a further optimization of the present invention, the topical dosage of the compound turpentine oil liniment is 2-5 times a day, with each application being 0.05-0.5g / kg.

[0015] The beneficial effects of this invention are as follows: The tiger nut extract prepared in this invention, when used in combination with turpentine oil, exhibits a certain synergistic effect, enhancing the efficacy of compound turpentine oil liniment in reducing serum inflammatory factors. Furthermore, it demonstrates good high-temperature stability. The tiger nut extract prepared through a combined enzymatic hydrolysis pretreatment process using cellulase and mannanase shows significantly better efficacy in reducing serum inflammatory factors, indicating a possible synergistic effect between cellulase and mannanase. This suggests that the compound turpentine oil liniment has a good therapeutic effect on arthritis. Detailed Implementation

[0016] The present application will now be described in further detail. It should be noted that the following specific embodiments are only used to further illustrate the present application and should not be construed as limiting the scope of protection of the present application. Those skilled in the art can make some non-essential improvements and adjustments to the present application based on the above application content.

[0017] I. Materials 1. pH adjuster: citric acid; 2. Cellulase: Purchased from Shaanxi Runfeng Biotechnology Co., Ltd., with a purity of 99% and an enzyme activity of 6.9 × 10⁻⁶. 4 U / g; 3. Mannanase: Purchased from Hubei Rishengchang New Material Technology Co., Ltd., with a purity of 99% and an enzyme activity of 7.3 × 10⁻⁶. 4 U / g.

[0018] 4. Tiger nuts: Tiger nuts are the underground tubers of the tiger nut (Cyperus esculentus var. sativus Boeckeler).

[0019] Unless otherwise specified, the methods used in this invention are conventional methods known to those skilled in the art, and the raw materials and reagents used are commercially available products.

[0020] II. Methods Example 1 (1) Preparation of tiger nut extract 1.1 Soak the screened, washed and dried tiger nut granules in purified water, adjust the pH to 5.5 using a pH adjuster, and soak at 45℃ for 1.5 hours; 1.2 The tiger nuts processed in step 1.1 were subjected to ultrasonic-assisted extraction. The ultrasonic-assisted extraction power was 350W, the frequency was 45kHz, the temperature was 55℃, and the time was 35min. Specifically, ultrasonic treatment was performed for 10s, followed by a 5s pause, and this cycle was repeated. The resulting extract was concentrated (to 20% of the original volume), freeze-dried, and pulverized to obtain a powdered tiger nut extract.

[0021] (2) Preparation of compound turpentine oil liniment In this embodiment, the compound turpentine liniment comprises the following raw materials in weight percentage: turpentine oil 8%, glycerin 52%, tiger pea extract 12%, soft soap 6%, camphor 0.8%, glucosamine 0.5%, chondroitin sulfate 0.8%, dimethyl sulfone 0.3%, oil-soluble azone 2%, lauramide propylamine oxide 0.3%, and purified water 17.3%.

[0022] The specific steps of the preparation method are as follows: 2.1. Soft soap, camphor powder, turpentine oil, glycerin, and tiger pea extract are ground in a colloid mill at 30-35℃ and 300-400 rpm for 1-2 hours to obtain a preliminary mixed emulsion. Then, glucosamine and chondroitin sulfate are added and ground at 30-35℃ and 200-300 rpm for 10-20 minutes. Finally, dimethyl sulfone, oil-soluble azone, and lauramide propylamine oxide are added and ground at 25-30℃ and 350-450 rpm for 20-35 minutes to obtain a mixture. 2.2 Transfer the mixture to a glass bottle, add purified water, and shake in a constant temperature water bath at 35-45℃ at 60r / min for 20-30min to ensure complete emulsification, thus obtaining the compound turpentine oil liniment.

[0023] Example 2 In this embodiment, the formulation of the compound turpentine liniment was adjusted. After adjustment, the compound turpentine liniment includes the following raw materials in the following mass percentages: 10% turpentine oil, 43.9% glycerin, 15% tiger nut extract (the preparation method of tiger nut extract is consistent with that in Example 1), 7.5% soft soap, 1.0% camphor, 0.5% glucosamine, 1% chondroitin sulfate, 0.3% dimethyl sulfone, 2% oil-soluble azone, 0.3% lauramidopropylamine oxide, and 18.5% purified water.

[0024] The preparation method of the compound turpentine oil liniment is consistent with that in Example 1.

[0025] Example 3 In this embodiment, the formulation of the compound turpentine liniment was adjusted. After adjustment, the compound turpentine liniment includes the following raw materials in the following mass percentages: 12% turpentine oil, 45% glycerin, 18% tiger nut extract (the preparation method of tiger nut extract is consistent with that in Example 1), 7% soft soap, 1.0% camphor, 0.5% glucosamine, 1% chondroitin sulfate, 0.3% dimethyl sulfone, 2% oil-soluble azone, 0.3% lauramidopropylamine oxide, and 12.9% purified water.

[0026] The preparation method of the compound turpentine oil liniment is consistent with that in Example 1.

[0027] Example 4 In this embodiment, based on Example 2, adjustments were made to the preparation process of tiger nut extract, as follows: (1) Preparation of tiger nut extract 1.1 Soak the screened, washed and dried tiger nut granules in purified water, adjust the pH to 5.0-6.0 using a pH adjuster, add an enzymatic hydrolysate (cellulase) at a dosage of 0.05-0.5 g / kg tiger nut granules, and soak at 40-50℃ for 1-2 hours; after soaking, inactivate the enzyme at 90-100℃ for 10-20 minutes. 1.2 The tiger nuts processed in step 1.1 were subjected to ultrasonic-assisted extraction. The ultrasonic-assisted extraction power was 350W, the frequency was 45kHz, the temperature was 55℃, and the time was 35min. Specifically, ultrasonic treatment was performed for 10s, followed by a 5s pause, and this cycle was repeated. The resulting extract was concentrated (to 20% of the original volume), freeze-dried, and pulverized to obtain a powdered tiger nut extract.

[0028] In addition, based on Example 4, this invention also explored technical solutions for preparing tiger nut extract using enzymatic hydrolysants with different ratios (Examples 5-6). The specific ratios of the enzymatic hydrolysants in Examples 5-6 are shown in Table 1: Table 1. Enzymatic hydrolysate formulations for preparing tiger nut extract in Examples 4-6 ; To investigate the effects of changes in different factors on the various properties of the tiger nut extract prepared in this invention and the compound turpentine oil liniment obtained by combining tiger nut extract and turpentine oil, this application also designed comparative examples 1-4, as follows: Comparative Example 1 In this comparative example, based on Example 6, the formulation of the compound turpentine liniment was adjusted. After adjustment, the compound turpentine liniment includes the following raw materials by mass percentage: turpentine 10%, glycerin 43.9%, soft soap 7.5%, camphor 1.0%, glucosamine 0.5%, chondroitin sulfate 1%, dimethyl sulfone 0.3%, oil-soluble azone 2%, laurylamidopropylamine oxide 0.3%, and purified water 33.5%.

[0029] The preparation method of the compound turpentine oil liniment is consistent with that in Example 1. Comparative Example 2 In this comparative example, based on Example 6, the formulation of the compound turpentine liniment was adjusted. After adjustment, the compound turpentine liniment includes the following raw materials by mass percentage: 10% turpentine oil, 43.9% glycerin, 15% soybean extract (the preparation method of soybean extract is consistent with that of Example 4), 7.5% soft soap, 1.0% camphor, 0.5% glucosamine, 1% chondroitin sulfate, 0.3% dimethyl sulfone, 2% oil-soluble azone, 0.3% lauramidopropylamine oxide, and 18.5% purified water.

[0030] Comparative Example 3 In this comparative example, based on Example 6, the formulation of the compound turpentine liniment was adjusted. After adjustment, the compound turpentine liniment includes the following raw materials by mass percentage: 10% turpentine oil, 43.9% glycerin, 15% pea extract (the preparation method of pea extract is consistent with that of Example 4), 7.5% soft soap, 1.0% camphor, 0.5% glucosamine, 1% chondroitin sulfate, 0.3% dimethyl sulfone, 2% oil-soluble azone, 0.3% lauramidopropylamine oxide, and 18.5% purified water.

[0031] Comparative Example 4 In this comparative example, based on Example 6, the formulation of the compound turpentine oil liniment was adjusted. After adjustment, the compound turpentine oil liniment includes the following raw materials by mass percentage: glycerin 43.9%, tiger nut extract 15% (the preparation method of tiger nut extract is consistent with that of Example 4), soft soap 7.5%, camphor 1.0%, glucosamine 0.5%, chondroitin sulfate 1%, dimethyl sulfone 0.3%, oil-soluble azone 2%, lauramide propylamine oxide 0.3%, and purified water 28.5%.

[0032] III. Experiment (1) Anti-inflammatory performance test Drugs subjected to high-temperature storage treatment: The compound turpentine oil liniments prepared in Examples 1-6 and Comparative Examples 1-3 were kept at a high temperature of 45°C for 60 days.

[0033] Drugs stored at room temperature: The compound turpentine oil liniments prepared in Examples 1-6 and Comparative Examples 1-3 were kept at a high temperature of 25°C for 60 days.

[0034] Experimental Methods: ICR mice weighing 50-60g were selected and randomly divided into 12 groups (Examples 1-6, Comparative Examples 1-4, negative control group, and positive control group). The hair around the right foot of each mouse was removed, and the joints were disinfected. The negative control group was injected with 0.04mL of physiological saline, while the others were injected with 0.04mL of complete Freund's adjuvant (CFA). Ten days later, a second injection of 0.04mL of complete Freund's adjuvant (CFA) was administered. The negative control group was injected with 0.04mL of physiological saline. The joints of the mice were observed. Changes at the joints were observed in mice in Examples 1-6, Comparative Examples 1-4, and the positive control group, indicating successful model construction. Fifteen days after successful model construction, mice in Examples 1-6 and Comparative Examples 1-4 were given medication (joint application, three times daily, 0.05-0.5 g / kg each time; 0.1 g / kg was used in this experiment) for 14 days. Serum levels of inflammatory factors (IL-1β, IL-6) (pg / mL) were measured, as shown in Table 2. Table 2. Statistical analysis of efficacy test data for compound turpentine oil liniment after high-temperature and room-temperature storage treatment. ; Experimental Conclusion: Based on the data analysis in Table 2, it can be seen that compared with the enzymatic pretreatment process without enzymatic hydrolysis or the enzymatic pretreatment process with a single enzyme, the tiger nut extract prepared by the enzymatic hydrolysis pretreatment process with cellulase and mannanase is significantly more effective in reducing inflammatory factors in serum. This indicates that there may be a synergistic effect between cellulase and mannanase. Furthermore, the tiger nut extract prepared by the method of this invention, when used in combination with turpentine oil, also exhibits a certain synergistic effect, which can enhance the effect of compound turpentine oil liniment in reducing inflammatory factors in serum and has good high-temperature stability.

[0035] (2) Clinical trials of drug efficacy Subjects: 350 dogs and cats with swollen joints in a pet hospital clinic.

[0036] Experimental procedure: The sick pets were randomly divided into 10 groups of 35 each and administered the drug.

[0037] The administration method was joint topical application, 3 times a day, with a dosage of 0.05-0.5 g / kg each time. In this application experiment, 0.1 g / kg was used for 14 consecutive days. During this period, observation was also conducted. No adverse reactions such as rapid breathing, strong cough, vomiting, diarrhea, convulsions, or coma were observed in any of the pets in all groups.

[0038] Experimental results statistics: ① Efficacy rate: During the experiment, clinical symptoms significantly improved and joint swelling basically disappeared; all were judged as effective, and the efficacy rate was calculated accordingly; ② Cure rate: During the experiment, pets were lively and their joints returned to normal after medication; all were judged as cured, and the cure rate was calculated accordingly; All results were statistically summarized in Table 3 below: Table 3. Statistical analysis of efficacy test data for compound turpentine oil liniment stored at room temperature. ; Experimental Conclusion: Based on the data analysis in Table 3, it can be seen that the compound turpentine oil liniments of Examples 1-6 all have good efficacy. Among them, the compound turpentine oil liniment of Example 6 has the best efficacy. This shows that the compound turpentine oil liniment of the present invention has good efficacy in the treatment of pet arthritis, and can effectively solve the problems of swelling, lameness, and pain caused by joint problems in animals. Through natural active factors and transdermal ingredients, it can quickly penetrate into the joint skin tissue to achieve the effect of reducing swelling and inflammation. The added analgesic ingredients can also effectively solve the discomfort caused by pain in pets.

[0039] The experiments described above only illustrate several embodiments of the present invention, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the present invention. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention, and these modifications and improvements all fall within the scope of protection of the present invention.

Claims

1. A compound turpentine oil liniment, characterized in that: The compound turpentine liniment comprises the following raw materials in the indicated weight percentages: turpentine oil 8-12%, glycerin 45-55%, tiger pea extract 12-18%, soft soap 5-10%, camphor 0.5-1.5%, glucosamine 0.1-1%, chondroitin sulfate 0.5-2%, dimethyl sulfone 0.1-1%, oil-soluble azone 0.5-2.5%, laurylamidopropylamine oxide 0.1-1%, and purified water 12-20%.

2. The compound turpentine oil liniment according to claim 1, characterized in that: The compound turpentine liniment comprises the following raw materials in weight percentage: 10% turpentine oil, 43.9% glycerin, 15% tiger pea extract, 7.5% soft soap, 1.0% camphor, 0.5% glucosamine, 1% chondroitin sulfate, 0.3% dimethyl sulfone, 2% oil-soluble azone, 0.3% lauramidopropylamine oxide, and 18.5% purified water.

3. The compound turpentine oil liniment according to claim 2, characterized in that: The method for obtaining the tiger nut extract is as follows: tiger nut granules that have been screened, washed and dried are soaked in purified water, the pH is adjusted to 5.0-6.0, and the mixture is soaked at 40-50℃ for 1-2 hours. Then, ultrasonic-assisted extraction is performed. The resulting extract is concentrated, freeze-dried and pulverized to obtain a powdered tiger nut extract.

4. A compound turpentine oil liniment according to claim 3, characterized in that: The ultrasonic-assisted extraction has a power of 300-500W, a frequency of 35-60kHz, a temperature of 50℃-60℃, and a time of 30-40min. Specifically, the ultrasonic-assisted extraction involves ultrasonic treatment for 10s, followed by a 5s pause, and this cycle is repeated.

5. A compound turpentine oil liniment according to claim 4, characterized in that: When the tiger nuts are soaked, an enzymatic hydrolysant is added to the purified water. The amount of enzymatic hydrolysant used per 1 kg of tiger nuts is 0.1-0.5 g. The enzymatic hydrolysant includes at least one of cellulase and mannanase. When an enzymatic hydrolysate is added to the purified water, it should be inactivated at 90-100℃ for 10-20 minutes after soaking.

6. A method for preparing a compound turpentine oil liniment as described in any one of claims 1-5, characterized in that: The specific steps are as follows: (1) The soft soap, camphor powder, turpentine oil, glycerin and tiger pea extract were first ground in a colloid mill to obtain a preliminary mixed emulsion; then glucosamine and chondroitin sulfate were added for a second grinding; then dimethyl sulfone, oil-soluble azone and laurylamidopropylamine oxide were added for a third grinding to obtain a mixture; (2) Transfer the mixture to a glass bottle, add purified water, and shake to emulsify it completely to obtain compound turpentine liniment.

7. The method for preparing a compound turpentine oil liniment according to claim 6, characterized in that: In step (1), the first grinding is carried out at 30-35℃ and 300-400r / min for 1-2 hours; The second grinding is carried out at 30-35℃ and 200-300r / min for 10-20min. The third grinding is carried out at 25-30℃ and 350-450r / min for 20-35 minutes.

8. The method for preparing a compound turpentine oil liniment according to claim 6, characterized in that: The shaking treatment is performed in a constant temperature water bath shaker at 35-45℃ for 60 r / min for 20-30 min.

9. The use of a compound turpentine oil liniment as described in any one of claims 1-5 in the preparation of a medicament for treating or improving arthritis in pets.

10. The application according to claim 9, characterized in that: The topical dosage of the compound turpentine oil liniment is 2-5 times a day, with each application being 0.05-0.5g / kg.