Essential oil for relieving pain caused by fascia nodules as well as preparation method and application of essential oil

Abstract

The invention belongs to the field of health care, and particularly relates to essential oil for relieving pain caused by fascia nodules and a preparation method and application thereof.The essential oil is prepared from, by weight, 15%-20% of core active essential oil, 0.5%-1.5% of a heat sensitizer, 1%-3% of an antioxidant, 0.5%-1.5% of trace functional components, 0.05%-0.1% of essence and the balance base oil. According to the invention, the raw materials are specifically selected and scientifically proportioned, and the effective components of the specific raw materials are extracted, so that the synergistic interaction among the components is realized. The prepared essential oil can quickly and effectively soften fascia nodules and body hardened tissues and relieve pain caused by the fascia nodules, has mild and comfortable warm feeling, greatly improves the efficiency and comfort of subsequent massage, physical therapy and other operations, and has wide application prospects in the fields of body physical therapy, exercise rehabilitation, daily health care and the like.

Description

Technical Field

[0001] This invention belongs to the field of health care, specifically relating to an essential oil for relieving pain caused by fascial nodules, its preparation method, and its application. Background Technology

[0002] Fascial nodules and local tissue hardening are common soft tissue problems caused by long-term strain, inflammation, or metabolic abnormalities, resulting in local tissue fibrosis or adhesions, often accompanied by symptoms such as pain and limited mobility.

[0003] Traditional treatments include massage and hot compresses, but these are relatively slow to take effect and may be accompanied by discomfort. Existing topical products are mainly massage oils, which contain capsaicin as the active ingredient. While capsaicin can promote local blood circulation and relieve muscle tension to some extent, its effectiveness in rapidly softening deep fascial nodules and hardened tissues is limited, and it also suffers from problems such as strong irritation and short duration of action.

[0004] Therefore, there is an urgent need to develop a topical product that is gentle on the skin, relieves pain, and can effectively soften deep fascia and nodules. Summary of the Invention

[0005] The purpose of this invention is to provide an essential oil for relieving pain caused by fascial nodules, its preparation method and application, which can effectively soften fascial nodules and hardened tissues in the body, and is accompanied by a mild and comfortable warming sensation, making it suitable for external use.

[0006] To achieve the above objectives, the present invention provides the following technical solution:

[0007] An essential oil for relieving pain caused by fascial nodules, the raw materials for which are prepared, by weight percentage, include 15%-20% core active essential oil, 0.5%-1.5% thermogenic agent, 1%-3% antioxidant, 0.5%-1.5% trace active ingredients, 0.05%-0.1% fragrance, and base oil to make up the balance to 100%.

[0008] Preferably, the core active essential oil includes one or more of turmeric root oil, ginger root oil, and lemon balm leaf oil; more preferably, it is turmeric root oil, ginger root oil, and lemon balm leaf oil.

[0009] Preferably, the mass ratio of turmeric root oil, ginger root oil, and lemon balm leaf oil is (1-3):(1-2):1; more preferably, it is 6:4:3.

[0010] By selecting turmeric root oil, ginger root oil, and lemon balm leaf oil as the core active essential oils, it is possible to relieve muscle tension and pain, soften fascial nodules, and shorten the softening time. This is likely because turmeric root oil is rich in curcumin compounds, which have anti-inflammatory and antioxidant effects, directly targeting fascial nodules and hardening caused by inflammation; ginger root oil is rich in gingerene, gingerol, and other effective ingredients, promoting local blood circulation, producing a warming sensation, relieving muscle tension and stiffness, and helping the active ingredients penetrate; lemon balm leaf oil has soothing, antispasmodic, and mild analgesic effects, helping to relieve muscle spasms and pain caused by fascial nodules. The three ingredients work synergistically through multiple pathways—anti-inflammatory, analgesic, and circulation-promoting—to relieve muscle tension, soften fascial nodules, and improve the efficiency of nodule softening. However, commercially available turmeric root oils are mainly prepared using traditional steam distillation, resulting in low curcumin content and easy degradation. Using large amounts of turmeric root oil to increase curcumin content in the essential oil would reduce the content of other components, affecting the oil's efficacy. Therefore, in order to achieve better fascia softening and pain relief, it is necessary to consider making your own turmeric root oil rich in curcumin.

[0011] Preferably, the method for preparing the turmeric root oil includes the following steps:

[0012] A1. After drying the turmeric root, pulverize it and pass it through a 40-mesh sieve to obtain turmeric root powder. Add water to adjust the moisture content to 80%-90% to obtain the enzymatic hydrolysis substrate. Add a compound enzyme for enzymatic hydrolysis, inactivate the enzyme, filter, and dry with hot air at 40℃ until the moisture content is ≤10% to obtain the enzymatic hydrolysis product.

[0013] A2. The enzymatic hydrolysis products were subjected to supercritical carbon dioxide fractional extraction to obtain the first and second products.

[0014] A3. Perform molecular distillation on the second product to obtain the purified product;

[0015] A4. Combine the first product and the purified product in a certain proportion to obtain the final product.

[0016] Preferably, in step A1, the complex enzyme includes one or more of cellulase, pectinase, and β-glucosidase; more preferably, it is cellulase, pectinase, and β-glucosidase.

[0017] Preferably, the amounts of cellulase, pectinase, and β-glucosidase added are 150-250 U / g of enzymatic hydrolysis substrate, 40-60 U / g of enzymatic hydrolysis substrate, and 20-40 U / g of enzymatic hydrolysis substrate, respectively.

[0018] In some preferred embodiments, the cellulase has an activity of approximately 100,000 U / g, the pectinase has an activity of approximately 30,000 U / g, and the β-glucosidase has an activity of approximately 2,000 U / g, and is derived from Shanghai Huashang Xiangyang Biotechnology Co., Ltd.

[0019] Preferably, the specific conditions for enzymatic hydrolysis are: temperature of 45-50℃, pH of 4.8-5.2, rotation speed of 150-200 rpm, and hydrolysis time of 3-4 hours.

[0020] Preferably, the specific conditions for enzyme inactivation are: temperature of 85-90℃ and time of 8-10 min.

[0021] Preferably, in step A2, the supercritical carbon dioxide fractional extraction includes a first stage of low-pressure extraction and a second stage of high-pressure enrichment.

[0022] Preferably, the conditions for the first stage of low-pressure extraction are: pressure of 25-28 MPa, temperature of 42-50℃, CO2 flow rate of 2-3 L / min, extraction time of 1-1.5 h, and the first product is collected by a primary separator.

[0023] Preferably, the specific parameters of the primary separator are: pressure of 6-10 MPa and temperature of 20-30℃.

[0024] Preferably, the conditions for the second-stage high-pressure enrichment are: pressure 38-40 MPa, temperature 55-65℃, CO2 flow rate 1.5-2 L / min, entrainer flow rate 5%-8% of CO2 flow rate, extraction time 2-2.5 h, and the second-stage product is collected by a secondary separator.

[0025] Preferably, the entrainer is an aqueous ethanol solution with a mass fraction of 95%.

[0026] Preferably, the specific parameters of the secondary separator are: pressure of 3-5 MPa and temperature of 35-45℃.

[0027] Preferably, in step A3, the specific conditions for molecular distillation are: feed rate of 0.8-1.2 mL / min, evaporation temperature of 180-200℃, system vacuum of 0.1-0.5 Pa, and condensation temperature of 43-47℃.

[0028] Preferably, in step A4, the mass ratio of the first product to the purified product is (7-9):(1-3).

[0029] Preferably, the specific conditions for compounding are: temperature of 40-45℃, protection from light, and stirring at 150-250 rpm for 30-60 minutes.

[0030] By making turmeric root oil in-house, the content of curcuminoids in the oil can be significantly increased, enhancing the final essential oil product's ability to soften hardened lumps and eliminate inflammation. This is likely because: firstly, turmeric root is treated with a complex enzyme hydrolysis process. Cellulase and pectinase work synergistically to disrupt the cell wall structure and decompose plant cell wall cellulose, while β-glucosidase hydrolyzes curcumin glucoside precursors, releasing free curcumin and increasing the curcumin content in the hydrolysis product. Then, supercritical carbon dioxide fractional extraction is used, with the low-pressure stage preserving the activity of the essential oil and the high-pressure stage combined with an entrainer to achieve targeted enrichment of curcumin, avoiding component degradation caused by high temperatures in traditional methods. Next, the curcumin fraction is purified to obtain a highly purified product. Finally, by mixing high-purity curcumin with whole essential oil in a certain proportion, the volatile anti-inflammatory and penetration-promoting components such as aromatic curcuminone in turmeric root and the purified anti-inflammatory and antioxidant component curcumin can be retained simultaneously. This results in a stronger synergistic anti-inflammatory and repairing effect with other components such as ginger root oil in the formula, while also enhancing the rapid softening effect. Furthermore, the turmeric root oil prepared by the method described in this invention can remove potential irritants and impurities, reduce the irritation of the essential oil, and is more suitable for use in massage, physiotherapy and other fields that require frequent or large-area application.

[0031] Preferably, the heat-sensitive agent comprises vanillyl butyl ether.

[0032] Preferably, the antioxidant includes tocopherol.

[0033] Preferably, the trace active ingredients include one or two of Tremella fuciformis polysaccharide and Artemisia argyi oil; more preferably, they are Tremella fuciformis polysaccharide and Artemisia argyi oil.

[0034] Preferably, the mass ratio of the tremella polysaccharide to the mugwort oil is (3-4):1; more preferably, it is 7:2.

[0035] By selecting vanillyl butyl ether as a heat-sensing agent and adding a trace amount of mugwort oil, it can work synergistically with ginger root oil to ensure a more significant and lasting warming effect. First, vanillyl butyl ether activates the heat receptors, then ginger essential oil promotes increased local blood flow, and mugwort oil warms the meridians and promotes blood circulation, reducing stiffness and recurrence, thus achieving a long-term warming effect.

[0036] Preferably, the base oil includes one or more of sunflower (HELIANTHUS ANNUUS) seed oil, peony (PAEONIA SUFFRUTICOSA) seed oil, and jojoba (SIMMONDSIA CHINENSIS) seed oil; more preferably, it is sunflower seed oil, peony seed oil, or jojoba seed oil.

[0037] Preferably, the mass ratio of sunflower seed oil, peony seed oil and jojoba seed oil is (5-6):(2-3):1; more preferably, it is 11:5:2.

[0038] By adding trace amounts of the active ingredient Tremella fuciformis polysaccharide, combined with the carrier oil peony seed oil, it can work synergistically with turmeric root oil to accelerate tissue repair. Jojoba oil and sunflower oil, as carrier oils, have excellent permeability, enabling them to carry active ingredients through the skin barrier to reach deeper fascia and hardened tissues, optimizing the absorption environment for active ingredients and achieving rapid softening of fascial nodules and hardened tissues, thus relieving pain.

[0039] The second aspect of this invention provides a method for preparing the essential oil for relieving pain caused by fascial nodules, comprising the following steps: mixing base oils and heating to 35-45°C, stirring at 200-400 rpm for 8-12 minutes, adding core active essential oils and Artemisia argyi oil sequentially, stirring for 15-25 minutes, adding a heat-sensitive agent and fragrance, stirring for 3-5 minutes, adding an antioxidant and Tremella fuciformis polysaccharide, stirring for 8-12 minutes, homogenizing, stopping heating, stirring and cooling to room temperature, standing for 24-48 hours, and filtering through a 200-mesh sieve to obtain the product.

[0040] Preferably, the specific conditions for homogenization are: stirring speed of 800-1200 rpm, homogenization time of 3-5 min, and homogenization pressure of 40-60 MPa.

[0041] The third aspect of this invention provides the application of the essential oil for relieving pain caused by fascial nodules, which can be used in the fields of physical therapy, sports rehabilitation, and daily health care.

[0042] Compared with the prior art, the advantages and beneficial effects of the present invention are as follows:

[0043] 1. This invention provides an essential oil for relieving pain caused by fascial nodules. By selecting core active essential oils and trace active ingredients, and preparing the oil using turmeric root oil, a synergistic effect among the components is achieved. The prepared essential oil can quickly and effectively soften fascial nodules and hardened tissues, relieving pain caused by fascial nodules, and providing a gentle and comfortable warming sensation. This greatly improves the efficiency and comfort of subsequent massage, physiotherapy, and other procedures. It also has multiple beneficial effects such as anti-inflammation, circulation promotion, soothing, and repair, and has broad application prospects in the fields of physical therapy, sports rehabilitation, and daily health care.

[0044] 2. This invention uses turmeric root oil, ginger root oil, and lemon balm leaf oil as core active essential oils, which can relieve muscle tension, soften fascial nodules, and relieve pain while shortening the softening time.

[0045] 3. By selecting vanillyl butyl ether as a heat-sensing agent and adding a trace amount of mugwort oil, this invention can work synergistically with ginger root oil to ensure a more significant and lasting warming effect.

[0046] 4. This invention, by adding trace amounts of the active ingredient Tremella polysaccharide and combining it with the carrier oil Peony seed oil, can work synergistically with Turmeric root oil to accelerate tissue repair.

[0047] 5. This invention, through the self-made turmeric root oil, can significantly increase the content of curcumin compounds in turmeric root oil, enhance the ability of the final essential oil product to soften hardened nodules and eliminate inflammation, thereby relieving pain caused by fascial nodules. Attached Figure Description

[0048] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below.

[0049] Figure 1 The images show a before (left) and after (right) comparison of the essential oil massage prepared in Example 1 7 days prior.

[0050] Figure 2 The images show a before (left) and after (right) comparison of the essential oil massage prepared in Example 1 three months prior.

[0051] Figure 3 The images show a before (left) and after (right) comparison of the essential oil massage prepared in Example 1 one month prior.

[0052] Figure 4 The images show a before (left) and after (right) comparison of the essential oil massage prepared in Example 1 three months prior.

[0053] Figure 5 The images show a before (left) and after (right) comparison of the essential oil massage prepared in Example 1 four months prior.

[0054] Figure 6 The images show a before (left) and after (right) comparison of the essential oil massage prepared in Example 1 three months prior. Detailed Implementation

[0055] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0056] All raw materials used in this invention are commercially available, specifically:

[0057] Ginger root oil, sourced from Jiangxi Baolin Natural Fragrance Co., Ltd.

[0058] Lemon balm leaf oil, Artemisia argyi oil, and peony seed oil are all from Guangzhou Fujie Biotechnology Co., Ltd.

[0059] Cellulase, with an enzyme activity of approximately 100,000 U / g; pectinase, with an enzyme activity of approximately 30,000 U / g; β-glucosidase, with an enzyme activity of approximately 2,000 U / g; all were obtained from Shanghai Huashang Xiangyang Biotechnology Co., Ltd.

[0060] Tremella polysaccharide, sourced from Shaanxi Panier Biotechnology Co., Ltd.

[0061] Sunflower seed oil, from Shanghai Zheyan Biotechnology Co., Ltd.

[0062] Jojoba seed oil, sourced from Guangdong Wengjiang Chemical Reagent Co., Ltd.

[0063] Example 1

[0064] This embodiment provides an essential oil for relieving pain caused by fascial nodules. The raw materials for its preparation, by weight percentage, include 18% core active essential oil, 1% thermogenic agent, 2% antioxidant, 1% trace active ingredients, 0.05% fragrance, and base oil to make up the balance to 100%.

[0065] The core active essential oils are turmeric root oil, ginger root oil, and lemon balm leaf oil, in a mass ratio of 6:4:3.

[0066] The preparation method of the turmeric root oil includes the following steps:

[0067] A1. After drying the turmeric root, pulverize it and pass it through a 40-mesh sieve to obtain turmeric root powder. Add water to obtain a mixture with a water content of 85wt% to obtain the enzymatic hydrolysis substrate. Add a compound enzyme for enzymatic hydrolysis, inactivate the enzyme, filter and dry it with hot air at 40℃ until the moisture content is 8% to obtain the enzymatic hydrolysis product.

[0068] A2. The enzymatic hydrolysis products were subjected to supercritical carbon dioxide fractional extraction to obtain the first and second products.

[0069] A3. Perform molecular distillation on the second product to obtain the purified product;

[0070] A4. Combine the first product and the purified product in a certain proportion to obtain the final product.

[0071] In step A1, the complex enzymes are cellulase, pectinase, and β-glucosidase, with addition amounts of 200 U / g of enzymatic hydrolysis substrate, 50 U / g of enzymatic hydrolysis substrate, and 30 U / g of enzymatic hydrolysis substrate, respectively.

[0072] The specific conditions for the enzymatic hydrolysis are: temperature 45℃, pH 5, rotation speed 180 rpm, and hydrolysis time 3.5 h.

[0073] The specific conditions for enzyme inactivation are: temperature 85℃ and time 10 min.

[0074] In step A2, the supercritical carbon dioxide fractional extraction includes a first stage of low-pressure extraction and a second stage of high-pressure enrichment.

[0075] The conditions for the first stage of low-pressure extraction were: pressure of 25 MPa, temperature of 45 °C, CO2 flow rate of 2.5 L / min, and extraction time of 1.5 h. The first product was collected by a primary separator.

[0076] The specific parameters of the primary separator are: pressure of 8 MPa and temperature of 25°C.

[0077] The conditions for the second-stage high-pressure enrichment were: pressure 38 MPa, temperature 60 °C, CO2 flow rate 2 L / min, entrainer flow rate 6% of CO2 flow rate, and extraction time 2.5 h. The second-stage product was collected by a secondary separator.

[0078] The entrainer is an aqueous solution of ethanol with a mass fraction of 95%.

[0079] The specific parameters of the secondary separator are: pressure 4MPa and temperature 40℃.

[0080] In step A3, the specific conditions for molecular distillation are: feed rate of 1 mL / min, evaporation temperature of 190°C, system vacuum of 0.3 Pa, and condensation temperature of 45°C.

[0081] In step A4, the mass ratio of the first product to the purified product is 4:1.

[0082] The specific conditions for compounding are: temperature 42℃, protection from light, and stirring at 200 rpm for 45 min.

[0083] The heat-sensitive agent is vanillyl butyl ether.

[0084] The antioxidant is tocopherol.

[0085] The trace active ingredients are Tremella polysaccharide and Artemisia argyi oil, with a mass ratio of 7:2.

[0086] The base oils are sunflower seed oil, peony seed oil, and jojoba seed oil in a mass ratio of 11:5:2.

[0087] The preparation method of the essential oil for relieving pain caused by fascial nodules is as follows: heat the base oil mixture to 40°C, stir at 300 rpm for 10 minutes, add the core active essential oil and mugwort oil in sequence, stir for 20 minutes, add the heat-sensing agent and fragrance, stir for 4 minutes, add the antioxidant and tremella polysaccharide, stir for 10 minutes, homogenize, stop heating, stir and cool to room temperature, let stand for 36 hours, and filter through a 200-mesh sieve to obtain the product.

[0088] The specific conditions for homogenization are: stirring speed of 1000 rpm, homogenization time of 4 min, and homogenization pressure of 50 MPa.

[0089] Example 2

[0090] The difference between this embodiment and Embodiment 1 is that the essential oil for relieving pain caused by fascial nodules is prepared by means of the following raw materials, by weight percentage: 20% core active essential oil, 1% thermosensitive agent, 2% antioxidant, 1% trace active ingredients, 0.05% fragrance, and base oil to make up the remainder to 100%.

[0091] Comparative Example 1

[0092] The difference between this comparative example and Example 1 is that the core active essential oils are ginger root oil and lemon balm leaf oil, with a mass ratio of 4:3.

[0093] Comparative Example 2

[0094] The difference between this comparative example and Example 1 is that the core active essential oils are turmeric root oil and lemon balm leaf oil, with a mass ratio of 2:1.

[0095] Comparative Example 3

[0096] The difference between this comparative example and Example 1 is that the trace active ingredient is Tremella polysaccharide.

[0097] Comparative Example 4

[0098] The difference between this comparative example and Example 1 is that the trace active ingredient is Artemisia argyi oil.

[0099] Comparative Example 5

[0100] The difference between this comparative example and Example 1 is that the base oils are sunflower seed oil and jojoba seed oil in a mass ratio of 11:2.

[0101] Comparative Example 6

[0102] The difference between this comparative example and Example 1 is as follows: The preparation method of the turmeric root oil is as follows: after drying the turmeric root, it is pulverized and passed through a 40-mesh sieve to obtain turmeric root powder. Water is added at a solid-liquid ratio of turmeric root powder to water of 1g:5mL. After soaking at room temperature for 35min, the water is heated to boil to generate steam. The water is kept at a slight boiling state of 95℃ and distilled continuously for 4h until no obvious oil droplets are precipitated in the condensate. The steam is cooled with 20℃ circulating cold water to convert it into an oil-water mixture. The mixture is allowed to stand for 2h to separate into layers. The upper layer of essential oil is taken and anhydrous sodium sulfate is added to adsorb the oil until the water content is 0.05%.

[0103] The essential oils prepared using the examples and comparative examples were tested.

[0104] Experimental subjects: 192 volunteers aged 25-55 years, with palpable fascial nodules or sclerotic tissue in the shoulder, neck, and lower back, with nodule diameter ≥5mm; VAS pain score of ≥3 points in the nodule area (0-10 points, the higher the score, the stronger the pain); no skin damage, allergies or other symptoms.

[0105] Experimental Methods: Volunteers were stratified by age, sex, and nodule diameter. Age was divided into three strata: 25-35 years old (young people with exercise-induced nodules); 36-45 years old (middle-aged people with chronic strain-induced nodules); and 46-55 years old (elderly people with degenerative nodules). Sex was categorized as male or female. Nodule diameter was categorized as 5-10 mm and >10 mm. The stratification variables were combined as follows: 3 (age) × 2 (sex) × 2 (nodule diameter) = 12 strata, with 16 participants in each stratum. From each stratum of 16 participants, 2 people were randomly selected to use the essential oil prepared in Example 1, and 2 people were randomly selected to use the essential oil prepared in Example 2, and so on, for a total of 8 groups, with 2 people per group per stratum. This process was repeated until all 12 strata were completed. The daily dosage for each person was 10 mL.

[0106] Assessment method: Volunteers self-reported the softening effect and VAS pain score of the nodule area 1 hour after application. The softening effect was assessed using a 5-point scale: 0 = no change, 1 = mild softening, 2 = moderate softening, 3 = significant softening, 4 = complete disappearance. The duration (min) of the immediate warming sensation was also assessed.

[0107] Evaluation criteria for usage results: The effect of the essential oil is judged by its ability to quickly soften fascial nodules and hardened tissue, and to generate heat. Results are categorized as significantly effective, effective, or ineffective.

[0108] Significant effect: Pain score reduced by ≥50%, softening effect score ≥3 after 1 hour, and duration of heat sensation ≥10 minutes.

[0109] Effective: Pain score reduced by 20%-50%, softening effect score of 2-3 after 1 hour, and heat sensation lasting 5-10 minutes.

[0110] Ineffective: Pain score decreased by <20%, softening effect score at 1 hour <2, duration of heat sensation <5 minutes or no heat sensation. Results are shown in Table 1.

[0111] Table 1 Measurement Results

[0112]

[0113]

[0114] Based on the above data, it can be seen that the essential oils prepared in Examples 1-2 of this invention have a significant and rapid softening effect, with noticeable softening effect within 1 hour, and a significant reduction in pain, with a long duration of heat sensation. Comparative Example 1, without the addition of turmeric root oil, could not quickly decompose fibrotic tissue using only ginger root oil and lemon balm leaf oil, resulting in a low softening effect score; Comparative Example 2, without the addition of ginger root oil, had insufficient heat intensity and limited pain relief; Comparative Example 3, without the addition of Tremella fuciformis polysaccharide, had a decreased absorption rate of active ingredients and a slower softening speed; Comparative Example 4, without the addition of Artemisia argyi oil, had an insufficient reduction in pain score; Comparative Example 5, without the addition of peony flower oil, had a decreased transdermal absorption rate of active ingredients and a low softening score; Comparative Example 6, using traditional steam distillation to prepare turmeric root oil, had a low content of curcumin and other substances, resulting in a slower softening speed and a shorter duration of heat sensation.

[0115] Comparison of before (left) and after (right) time periods using the essential oil massage prepared in Example 1, see... Figures 1-6 .from Figures 1-6 It can be seen that the use of essential oil massage can soften nodules and has multiple effects such as anti-inflammatory, circulation-promoting, soothing, and repairing.

[0116] The curcumin content in the turmeric root oil prepared in Example 1 and Comparative Example 6 was determined according to the HPLC method in the 2020 edition of the Chinese Pharmacopoeia. HPLC test conditions: column: C18 column (250 mm × 4.6 mm, 5 μm); mobile phase: acetonitrile-4% glacial acetic acid (45:55, v / v); flow rate: 1.0 mL / min; detection wavelength: 425 nm; column temperature: 30 ℃; injection volume: 20 μL. The results are shown in Table 2.

[0117] Table 2: Test Results

[0118] Curcumin content (mg / g) Example 1 8.23 Comparative Example 6 1.21

[0119] As shown in Table 2, the method for preparing turmeric root oil in Example 1 of this invention significantly increases the curcumin content compared to the traditional steam distillation method, thereby greatly improving the performance of the prepared essential oil. Therefore, the method described in this application can significantly increase the curcumin content in turmeric root oil; the essential oil prepared using the raw materials and method described in this application can quickly and effectively soften fascial nodules and hardened tissues, relieve pain caused by fascial nodules, and provide a gentle and comfortable warming sensation, greatly improving the efficiency and comfort of subsequent massage, physiotherapy, and other procedures.

[0120] The above description represents the preferred embodiments of the present invention. It should be noted that those skilled in the art can make various improvements and modifications without departing from the principles of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. An essential oil for relieving pain caused by fascial nodules, characterized in that, The raw materials used in its preparation, by weight percentage, include 15%-20% core active essential oil, 0.5%-1.5% thermosensitive agent, 1%-3% antioxidant, 0.5%-1.5% trace active ingredients, 0.05%-0.1% fragrance, and base oil to make up the balance to 100%. The core active essential oils include turmeric root oil, ginger root oil, and lemon balm leaf oil; the trace active ingredients include tremella polysaccharide and mugwort oil.

2. The essential oil for relieving pain caused by fascial nodules according to claim 1, characterized in that, The mass ratio of turmeric root oil, ginger root oil, and lemon balm leaf oil is (1-3):(1-2):

1.

3. The essential oil for relieving pain caused by fascial nodules according to claim 2, characterized in that, The method for preparing the turmeric root oil includes the following steps: A1. After drying the turmeric root, pulverize it and sieve it to obtain turmeric root powder. Add water to adjust the moisture content to 80%-90% to obtain the enzymatic hydrolysis substrate. Add a compound enzyme for enzymatic hydrolysis, inactivate the enzyme, filter and dry with hot air until the moisture content is ≤10% to obtain the enzymatic hydrolysis product. A2. The enzymatic hydrolysis products were subjected to supercritical carbon dioxide fractional extraction to obtain the first and second products. A3. Perform molecular distillation on the second product to obtain the purified product; A4. Combine the first product and the purified product in a certain proportion to obtain the final product.

4. The essential oil for relieving pain caused by fascial nodules according to claim 3, characterized in that, In step A1, the complex enzymes include cellulase, pectinase, and β-glucosidase.

5. The essential oil for relieving pain caused by fascial nodules according to claim 4, characterized in that, The amounts of cellulase, pectinase, and β-glucosidase added are 150-250 U / g of enzymatic hydrolysis substrate, 40-60 U / g of enzymatic hydrolysis substrate, and 20-40 U / g of enzymatic hydrolysis substrate, respectively.

6. The essential oil for relieving pain caused by fascial nodules according to claim 3, characterized in that, In step A2, the supercritical carbon dioxide fractional extraction includes a first stage of low-pressure extraction and a second stage of high-pressure enrichment.

7. The essential oil for relieving pain caused by fascial nodules according to claim 6, characterized in that, The conditions for the first stage of low-pressure extraction are: pressure of 25-28 MPa, temperature of 42-50℃, CO2 flow rate of 2-3 L / min, extraction time of 1-1.5 h, and the first product is collected by a primary separator.

8. The essential oil for relieving pain caused by fascial nodules according to claim 6, characterized in that, The conditions for the second-stage high-pressure enrichment are: pressure 38-40 MPa, temperature 55-65℃, CO2 flow rate 1.5-2 L / min, entrainer flow rate 5%-8% of CO2 flow rate, extraction time 2-2.5 h, and the second-stage product is collected through a secondary separator.

9. A method for preparing an essential oil for relieving pain caused by fascial nodules according to any one of claims 1 to 8, characterized in that, Includes the following steps: Heat the base oil mixture to 35-45℃ and stir at 200-400 rpm for 8-12 minutes. Add the core active essential oil and mugwort oil in sequence and stir for 15-25 minutes. Add the heat-sensitive agent and fragrance and stir for 3-5 minutes. Add the antioxidant and tremella polysaccharide and stir for 8-12 minutes. Homogenize, stop heating, stir and cool to room temperature. Let stand for 24-48 hours and filter through a 200-mesh screen to obtain the final product.

10. The application of an essential oil for relieving pain caused by fascial nodules according to any one of claims 1 to 8 in the fields of physical therapy, sports rehabilitation, and daily health care.

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