Anti-wrinkle soothing composition as well as application and cosmetic thereof

Abstract

The invention belongs to the field of cosmetics, and discloses an anti-wrinkle soothing composition, application of the anti-wrinkle soothing composition and a cosmetic. Comprising the following components in parts by weight: 15-20 parts of hydroxypropyl tetrahydropyrantriol, 10-15 parts of tetrahydromethylpyrimidine carboxylic acid, 5-10 parts of a leukocyte extract, 5-20 parts of a leontopodium alpinum callus extract solution and the balance of a bifidus yeast fermentation product filtrate, through reasonable compounding of the five components, synthesis of collagen and elastin can be effectively promoted, and good anti-wrinkle and soothing effects are achieved.

Description

Technical Field

[0001] This application relates to the field of cosmetics, and more particularly to an anti-wrinkle and soothing composition and its use in cosmetics. Background Technology

[0002] The skin consists of three layers: the epidermis, dermis, and subcutaneous tissue. The dermis contains abundant collagen and elastin, proteins that keep the skin elastic and firm. As people age or are affected by external environmental factors, the production of these proteins in the skin gradually decreases, leading to a loss of elasticity and the formation of wrinkles. Wrinkles are the most direct manifestation of skin aging. Therefore, to slow down skin aging, more and more manufacturers are developing anti-wrinkle and firming cosmetics to reduce or prevent wrinkles.

[0003] Prior art 1: Chinese patent application 201510725136.3 discloses a topical anti-wrinkle composition for skin, consisting of rhEGF, hydrolyzed silk protein, artemia extract, and brewer's yeast extract. Combining hydrolyzed silk protein, artemia extract, and brewer's yeast extract with rhEGF effectively prevents rhEGF inactivation, significantly improves rhEGF efficiency, and promotes rhEGF absorption while reducing rhEGF loss during penetration. rhEGF (recombinant human epidermal growth factor) is a bioactive polypeptide synthesized through gene recombination technology. Its structure is highly similar to that of natural human epidermal growth factor, composed of 53 amino acids, with a molecular weight of approximately 6000-6200 Daltons. It possesses excellent heat and acid resistance. By binding to cell surface receptors (such as EGFR), it activates intracellular signaling pathways, promotes RNA, DNA, and protein synthesis, thereby accelerating cell proliferation, migration, and differentiation, and supporting tissue repair and regeneration.

[0004] However, EGF mainly targets the epidermis, primarily focusing on epidermal repair. It is suitable for skin problems such as thin epidermis, sensitive skin, and superficial fine lines, achieving a certain anti-wrinkle effect. However, it has a lower effect on deeper skin aging, collagen loss, and other skin wrinkles.

[0005] Therefore, there is a need to develop an anti-wrinkle composition that can act not only on the epidermis for skin care, but also provide a more comprehensive anti-wrinkle effect. Summary of the Invention

[0006] To address the aforementioned issues, one objective of this application is to provide an anti-wrinkle and soothing composition that, through the rational combination of five components—hydroxypropyl tetrahydropyranotriol, tetrahydromethylpyrimidine carboxylic acid, Bifida ferment filtrate, leukocyte extract, and Edelweiss callus extract—can act simultaneously on both the epidermis and dermis, thereby exerting a comprehensive anti-wrinkle effect on the skin.

[0007] Another objective of this application is to provide the use of an anti-wrinkle and soothing composition in the preparation of cosmetics, wherein when the anti-wrinkle and soothing composition is applied to the skin as an active ingredient in cosmetics, it can effectively promote the anti-wrinkle and soothing effects of cosmetics.

[0008] Additionally, this application also provides a cosmetic product containing the anti-wrinkle and soothing composition of this application.

[0009] To achieve the above objectives, this application discloses an anti-wrinkle and soothing composition comprising the following components: 15-20 parts of hydroxypropyl tetrahydropyranotriol, 10-15 parts of tetrahydromethylpyrimidine carboxylic acid, 5-10 parts of leukocyte extract, 5-20 parts of Edelweiss callus extract solution, and 35-65 parts of Bifida ferment filtrate.

[0010] Hydroxypropyltetrahydropyranotriol and tetrahydromethylpyrimidine carboxylic acid can promote the secretion of fibroblast growth factor (FGF) by fibroblasts, activate the FGFR signaling pathway, promote the synthesis of collagen and elastin, accelerate cell proliferation and damage repair processes, and also reduce the damage of collagen and elastic fibers to oxidative stress by neutralizing free radicals, thus delaying the photoaging process. The amino acids (such as glycine and proline) and lactic acid in the Bifida ferment lysate filtrate can activate the fibroblast FGFR signaling pathway, increase the secretion of type I collagen, inhibit the activity of matrix metalloproteinases (MMP-1 / -3) and upregulate TIMP-1 expression, reduce collagen fiber breakage. The active ingredients in the filtrate can also scavenge reactive oxygen species (ROS) and free radicals induced by ultraviolet radiation, block the oxidation chain reaction, prevent lipid peroxidation and collagen oxidative damage, and improve skin elasticity. The leukocyte extract contains epidermal growth factor (EGF) and fibroblast growth factor (FGF), which can stimulate the proliferation and differentiation of epidermal basal cells, accelerate the renewal of aging stratum corneum, and promote the regeneration efficiency of damaged epidermis. It also stimulates the synthesis of collagen and elastin fibers, effectively repairing skin problems caused by external environmental damage (such as ultraviolet radiation) and significantly improving signs of skin aging. The edelweiss callus extract contains polyphenols and flavonoids that can neutralize free radicals, inhibit matrix metalloproteinase (MMPS) activity, reduce collagen loss, and promote the synthesis of type I and type III collagen, improving skin elasticity. It also stimulates the secretion of vascular endothelial growth factor (VEGF), improves dermal microcirculation, and provides oxygen and nutrient support for skin repair. Through the rational combination of various ingredients, the composition can synergistically exert a better anti-wrinkle and soothing effect.

[0011] Preferably, the anti-wrinkle and soothing composition may also contain appropriate amounts of inositol, ceramide NP, asiaticoside, rose extract, and desert rose leaf cell extract to enhance the anti-wrinkle and soothing effect of the composition.

[0012] In the technical solution of this application, the Edelweiss callus extract solution can be made from commercially available raw materials; preferably, the Edelweiss callus extract solution of this application contains 20wt% Edelweiss callus extract.

[0013] In addition, the use of the anti-wrinkle and soothing composition described above as a cosmetic active ingredient is also disclosed.

[0014] In addition, a cosmetic product is disclosed, which contains 1 to 10 wt% of the anti-wrinkle and soothing composition as described above.

[0015] Preferably, the cosmetic is one of the following: skin care water, serum, lotion, cream, and mask.

[0016] The beneficial effects of this application are:

[0017] This application provides an anti-wrinkle and soothing composition, which, through the rational combination of five components—hydroxypropyl tetrahydropyranotriol, tetrahydromethylpyrimidine carboxylic acid, Bifida ferment filtrate, leukocyte extract, and Edelweiss callus extract—can effectively promote the synthesis of collagen and elastin, achieving a good anti-wrinkle and soothing effect. Attached Figure Description

[0018] The present application will be described below with reference to the accompanying drawings and embodiments:

[0019] Figure 1 Photographs of cell scratches at 0 h in the blank control group during the cell scratch healing test;

[0020] Figure 2 Photographs of cell scratches in the blank control group at 24 hours in the cell scratch healing test;

[0021] Figure 3 Photographs of cell scratches in the blank control group at 48 hours in the cell scratch healing test;

[0022] Figure 4 Photographs of cell scratches in the positive control group at 0h in a cell scratch healing test;

[0023] Figure 5 Photographs of cell scratches in the positive control group at 24 hours in a cell scratch healing test;

[0024] Figure 6 Photographs of cell scratches in the positive control group at 48 hours in a cell scratch healing test;

[0025] Figure 7 Photographs of cell scratches at 0h in Example 1 group during the cell scratch healing test;

[0026] Figure 8Photographs of cell scratches in Example 1 group at 24 hours in the cell scratch healing test;

[0027] Figure 9 Cell scratch images of Example 1 group after 48 hours in the cell scratch healing test. Detailed Implementation

[0028] In the description of this application, it should be noted that, unless specific conditions are specified in the examples, conventional conditions or conditions recommended by the manufacturer are followed. Reagents or instruments whose manufacturers are not specified are all commercially available products.

[0029] Supplier information and / or product information

[0030] Hydroxypropyltetrahydropyranotriol: Tianjin Taipu Pharmaceutical Co., Ltd., content 100wt%;

[0031] Tetrahydromethylpyrimidine carboxylic acid: Nanjing Xianda Pharmaceutical Technology Co., Ltd., content 100wt%;

[0032] Leukocyte extract: Microsec Biotechnology (Yantai) Co., Ltd., content 100wt%;

[0033] Bifida Ferment Lysate Ferment Filtrate: Guangdong Dimei New Material Technology Co., Ltd., composed of 99wt% Bifida Ferment Lysate Ferment Filtrate, 0.5wt% p-hydroxyacetophenone, and 0.5wt% 1,2-hexanediol;

[0034] Edelweiss callus extract solution: Ansaibo (Chongqing) Biotechnology Co., Ltd., composed of 20wt% Edelweiss callus extract and 80wt% glycerin;

[0035] Desert Rose Leaf Cell Extract Solution: Guangzhou Xincheng Meike Biotechnology Co., Ltd., composed of 89wt% glycerol, 10wt% desert rose (ADENIUM OBESUM) leaf cell extract, 0.5wt% p-hydroxyacetophenone, and 0.5wt% 1,2-hexanediol.

[0036] It should be noted that the technical solution of this application can be achieved by purchasing other commercially available raw materials, and is not limited to the aforementioned raw material suppliers.

[0037] Examples 1-3

[0038] An anti-wrinkle and soothing composition, the components of which are shown in Table 1 by weight percentage:

[0039] Table 1. Ingredients of the Anti-wrinkle and Soothing Composition

[0040]

[0041] Comparative Examples 1-6

[0042] An anti-wrinkle and soothing composition, the components of which are shown in Table 2 by weight:

[0043] Table 2. Ingredients of the Anti-wrinkle and Soothing Composition

[0044]

[0045]

[0046] Performance testing:

[0047] I. Hyaluronidase Activity Test

[0048] 1. Experimental Principle

[0049] Hyaluronic acid, as an important natural moisturizing substance, plays a vital role in maintaining the volume of the extracellular matrix and normal skin physiological functions. However, hyaluronic acid can be specifically broken down by hyaluronidase, and its products promote skin inflammation, leading to increased histamine production in the body. Furthermore, hyaluronidase expression is also associated with most IgE-mediated type I and T-cell-mediated type IV hypersensitivity reactions. Inhibiting hyaluronidase activity can, to some extent, help suppress the development of skin inflammation and allergic reactions, and improve the irritated state of the skin caused by inflammation and allergic reactions. Therefore, inhibition of hyaluronidase activity is often used as an important indicator for evaluating the soothing and moisturizing effects of products.

[0050] Hyaluronidase breaks the glycosidic bond between N-acetylglucosamine and D-glucuronic acid in the hyaluronic acid (HA) sugar chain. The resulting reducing sugar can reduce DNS to an amino compound under alkaline conditions. After boiling in a water bath to allow for sufficient color development, its absorbance can be measured at 540 nm, thereby calculating the hyaluronidase activity.

[0051] 2. Materials and Methods

[0052] 2.1 Instruments and Equipment

[0053] Microbalance, water bath, microplate reader.

[0054] 2.2 Reagents and Materials

[0055] Hyaluronidase, hyaluronic acid, positive control: dexamethasone, DNS reagent.

[0056] 3. Operation process

[0057] 3.1 Test Operation

[0058] Test substance treatment: stock solution;

[0059] Refer to the sample addition grouping list (Table 1) and use 2mL centrifuge tubes for the experiment; each group has 3 parallel tubes.

[0060] Table 1. Sample Addition Grouping List

[0061]

[0062] After the test tube has cooled, shake well and add 150 μL of the reaction solution to a 96-well plate. Measure the absorbance at OD540 nm using an ELISA reader.

[0063] 3.2 Calculation of Results

[0064] Calculate the hyaluronidase activity inhibition rate:

[0065]

[0066] In the formula:

[0067] T—Absorbance of the sample group, i.e., the absorbance of the solution after the sample inhibits the reaction between hyaluronidase and hyaluronic acid;

[0068] T0—The background absorbance of the sample;

[0069] The absorbance of the C-enzyme reaction group is the absorbance of the reaction between hyaluronidase and hyaluronic acid without the addition of a sample.

[0070] C0 — absorbance of the solvent background group.

[0071] Examples 1-3 and Comparative Examples 1-7 were tested as test subjects according to the above method, and the results are shown in Table 2.

[0072] Table 2. Hyaluronidase activity inhibition effects of Examples 1-3 and Comparative Examples 1-7

[0073]

[0074]

[0075] According to the results in Table 2:

[0076] Based on the comparison of data from Examples 1-3 and the positive control data, it can be seen that the composition of this application has excellent skin soothing and moisturizing effects, and the hyaluronidase activity inhibition rate is at a high level.

[0077] A comparison of the data from Example 1 and Comparative Examples 1-3 shows that the present application, through the combination of hydroxypropyl tetrahydropyranotriol, tetrahydromethylpyrimidine carboxylic acid, Bifida ferment filtrate, leukocyte extract, and Edelweiss callus extract, can effectively improve the hyaluronidase activity inhibition rate of the composition. Although the improvement in hyaluronidase activity inhibition rate of Example 1 is lower than that of Comparative Examples 1 and 2, a comparison of the data from Example 1 and Comparative Examples 1 and 3 shows that the combination of leukocyte extract with hydroxypropyl tetrahydropyranotriol, tetrahydromethylpyrimidine carboxylic acid, and Bifida ferment filtrate does not effectively improve the hyaluronidase activity inhibition rate of the composition. It is speculated that the leukocyte extract itself does not have a significant effect on improving the hyaluronidase activity inhibition rate, but it produces a certain degree of synergistic effect when combined with hydroxypropyl tetrahydropyranotriol, tetrahydromethylpyrimidine carboxylic acid, Bifida ferment filtrate, and Edelweiss callus extract.

[0078] Furthermore, based on the data from comparative examples 4-7, it can be inferred that leukocyte extract and Rosa desertis leaf cell extract solution had no effect on improving the inhibition rate of hyaluronidase activity, while Edelweiss callus extract solution had a more significant effect on improving the inhibition rate of hyaluronidase activity.

[0079] II. Type I Collagen Content Test

[0080] 1. Experimental Principle

[0081] During their proliferation and division, cultured dermal fibroblasts secrete substances such as collagen. The efficacy of test substances in firming and anti-wrinkle cell function can be evaluated by measuring the expression of related proteins after administration. This document uses human fibroblasts (HDF) and a human enzyme-linked immunosorbent assay (ELISA) kit to detect the test substance's markers: type I collagen (ColI) and elastin (ELT). The analyte specifically binds to the antibody coated on the ELISA plate, and then to the substrate-labeled antibody. The substrate is catalyzed by the enzyme to generate a colored product. The concentration of the analyte is positively correlated with the intensity of the colored product. The concentration of the analyte is calculated by measuring the optical density (OD value) at 450 nm using an ELISA reader.

[0082] 2. Instruments and Equipment

[0083] Microbalance, CO2 incubator, microplate reader, water bath, biosafety cabinet.

[0084] 3. Reagents and Materials

[0085] Cell Counting Kit-8 reagents; BASIC H-DMEM, 4.5g Liter Glucose; FBS; 0.25% Trypsin-EDTA; COLI ELISA KIT; ELT ELISA KIT human fibroblasts.

[0086] 4. Operation process

[0087] 4.1 Pretreatment of test substance

[0088] The original sample solution was sterilized by filtration using a 0.22 μm filter, and then diluted to a concentration of 0.1562% using culture medium.

[0089] 4.2 Type I Collagen Secretion Test

[0090] After cell counting, the cells were diluted to the required concentration and added to 96-well microplates. After 24 hours of plating, when the cell confluence reached 40-60%, the test concentration of the reagent was added and incubated for another 24 hours. 200 μL of cell culture supernatant was collected from each well into a 1.5 mL sterile centrifuge tube, and the assay was performed according to the instructions for use of the COLI ELISA kit and the ELT ELISA kit.

[0091] 4.3 Result Calculation

[0092] A curve was plotted with absorbance (OD) value on the ordinate (Y) and the corresponding concentration of the analyte standard on the abscissa (X). The concentration of the analyte in the sample could be calculated from the standard curve based on its OD value. The final result of the analyte concentration was taken as the average value of the three replicates for each group.

[0093] Examples 1-3 and Comparative Examples 1-7 were tested according to the above method, and the results are shown in Table 3.

[0094] Table 3. Effects of Examples 1-3 and Comparative Examples 1-7 on type I collagen secretion in HDF.

[0095] Type I collagen content (ng / ml) Example 1 77.90 Example 2 73.15 Example 3 81.54 Comparative Example 1 36.93 Comparative Example 2 48.47 Comparative Example 3 42.87 Comparative Example 4 53.24 Comparative Example 5 66.12 Comparative Example 6 50.96 Comparative Example 7 37.88

[0096] According to the results in Table 3:

[0097] As can be seen from Examples 1-3, the composition of this application still exhibits a high effect on the secretion of type I collagen even at a low concentration of 0.1562%, indicating that the composition of this application can effectively increase the content of type I collagen, thereby achieving the effect of anti-wrinkle skin.

[0098] A comparison of the data from Example 1 and Comparative Examples 1-3 shows that the present application, through the compounding of hydroxypropyl tetrahydropyranotriol, tetrahydromethylpyrimidine carboxylic acid, Bifida ferment filtrate, leukocyte extract, and Edelweiss callus extract, can significantly increase the type I collagen content of the composition. In contrast, in Comparative Examples 1-3, where leukocyte extract and / or Edelweiss callus extract were lacking, the type I collagen content of the compositions showed a significant decrease. The possible reasons for this are as follows:

[0099] (1) The combination of hydroxypropyl tetrahydropyranotriol, tetrahydromethylpyrimidine carboxylic acid, Bifida ferment filtrate, leukocyte extract and Edelweiss callus extract has a significant synergistic effect, and none of the five components can be omitted.

[0100] (2) There is a synergistic effect between the leukocyte extract and the Edelweiss callus extract, which can significantly enhance the anti-wrinkle effect of the composition.

[0101] Based on the above speculation, this application verified the results using Comparative Examples 4-7. A comparison of the data from Example 1 and Comparative Example 4 shows that when the *Edelweiss* callus extract solution in the composition of this application is replaced with *Rosa desertica* leaf cell extract solution, the type I collagen content of the composition significantly decreases. However, data from Comparative Examples 5 and 6 show that, with the absence of hydroxypropyl tetrahydropyranotriol and tetrahydromethylpyrimidine carboxylic acid and a fixed concentration of leukocyte extract and Bifida ferment filtrate, Comparative Example 5 shows a higher increase in type I collagen content than Comparative Example 6, with a significant difference, especially when the concentration of *Rosa desertica* leaf cell extract in the composition is lower than that of *Edelweiss* callus extract. This indicates that the leukocyte extract and *Edelweiss* callus extract have significantly different concentrations. The extracts of *Edelweiss* callus tissue do not have a synergistic effect, or the synergistic effect is not as obvious as the technical solution of combining leukocyte extract and *Rosa desertica* leaf cell extract; however, Comparative Example 4 still shows a significant decrease in type I collagen content compared to Example 1. According to the data of Comparative Example 7, it can be seen that the combination of hydroxypropyl tetrahydropyranotriol, tetrahydromethylpyrimidine carboxylic acid, Bifida ferment filtrate, and *Rosa desertica* leaf cell extract does not have a good anti-wrinkle effect. Therefore, it can be seen that the composition of this application, which is a combination of hydroxypropyl tetrahydropyranotriol, tetrahydromethylpyrimidine carboxylic acid, Bifida ferment filtrate, leukocyte extract, and *Edelweiss* callus tissue extract, has a significant synergistic effect and can significantly improve the anti-wrinkle efficacy of the composition. All five components are indispensable.

[0102] III. Elastase Activity Test

[0103] 1. Experimental Principle

[0104] Elastin plays a crucial role in maintaining youthful skin, and its loss is a major cause of skin aging. Inhibiting elastase activity can effectively slow down the breakdown of elastin and maintain skin elasticity. Elastase catalyzes the hydrolysis of hydroxyl groups in the polypeptide bonds of various amino acids. Therefore, when it reacts with the substrate N-succinyl-alanine-alanine-alanine-p-nitroaniline, a colored substance is produced, the absorbance of which can be measured at 410 nm using a spectrophotometer. Based on the change in absorbance, the inhibitory effect of the test substance on elastase activity can be calculated, and the firming and anti-wrinkle effects of the test substance can be evaluated.

[0105] 2. Materials and Methods

[0106] 2.1 Instruments and Equipment

[0107] Precision balance with an accuracy of 0.01%, microplate reader.

[0108] 2.2 Reagents and Materials

[0109] Elastase, AAAPAN: N-succinyl-alanine-alanine-alanine-p-nitroaniline, positive control: EGCG, buffer: Tris-HCl pH 8.0.

[0110] 3. Operation process

[0111] 3.1 Test Operation

[0112] The elastase and the chromogenic substrate N-succinyl-alanine-alanine-alanine-p-nitroaniline were prepared into a working solution using buffer and should be prepared immediately before use.

[0113] The positive control EGCG was prepared with buffer solution and diluted 1 / 2 times to a series of concentrations to verify the experimental system.

[0114] Test substance: Original solution of the test substance.

[0115] Referring to Table 4, experiments were conducted using 96-well microplates, with four groups: sample group (T), sample background group (To), enzyme reaction group (C), and solvent background group (Co). The positive control group was treated the same as the sample group. Each group was run in triplicate.

[0116] Table 4 Grouping of elastase samples by solution addition

[0117]

[0118] Immediately use an ELISA reader for detection, with the parameters set as follows: slow oscillation for 10 seconds, absorbance measured at OD410nm. Export the file after the instrument completes the reading.

[0119] 3.2 Calculation of Results

[0120] Elastase activity inhibition rate:

[0121]

[0122] In the formula:

[0123] T represents the absorbance of the sample group, i.e., the absorbance of the solution after the sample affects the reaction between elastase and the substrate AAAPAN.

[0124] T0—The background absorbance of the sample;

[0125] The absorbance of the C-enzyme reaction group is the absorbance of the reaction between elastase and substrate AAAPAN without the addition of sample.

[0126] C0 — absorbance of the solvent background group.

[0127] The test sample of Example 1 was tested according to the above method, and the results are shown in Table 5.

[0128] Table 5. Inhibitory effect of elastase activity of the composition in Example 1.

[0129] elastase activity inhibition rate % Positive control 76.32 Example 1 11.71

[0130] According to the results in Table 5:

[0131] Elastase is also one of the mechanisms by which skin anti-wrinkle effects are manifested. Example 1 of this application also has a certain elastase activity inhibition rate, showing anti-wrinkle effects based on elastase inhibition. However, compared with the mechanism of increasing collagen content, its effect is not so significant. This indicates that the composition of this application mainly increases type I collagen content and secondarily inhibits elastase activity in terms of skin anti-wrinkle performance, thereby achieving significant anti-wrinkle effects.

[0132] IV. Cell scratch healing test

[0133] 1. Principle

[0134] Cell migration refers to the movement of cells after receiving migration signals or sensing stimulation from certain substances. Increased cell migration ability indicates a wound-healing effect. This experiment evaluates the repair effect of the test substance by calculating the scratch healing rate based on the speed of cell scratch healing.

[0135] 2. Instruments and Equipment

[0136] Microbalance, biosafety cabinet, carbon dioxide incubator, microplate reader, water bath.

[0137] 3. Reagents and Materials

[0138] 3.1 Reagents

[0139] Cell Counting Kit-8 reagents; BASICH-DMEM, 4.5g Liter Glucose; FBS; 0.25% Trypsin-EDTA; Positive sample: EGF.

[0140] 3.2 Cell lines

[0141] Human immortalized keratinocytes HaCaT.

[0142] 4. Operation process

[0143] 4.1 Pretreatment of test substance

[0144] The original sample solution was sterilized by filtration using a 0.22 μm filter, and then diluted to a concentration of 0.3125% using culture medium.

[0145] 4.2 Cell Scratch Assay

[0146] Cells were seeded in 24-well plates, with the cell count adjusted to achieve confluence every other day. After 24 hours of adherent culture, straight scratches were made on the bottom of the plate, ensuring consistent pressure, angle, and thickness. Floating cells were washed away with PBS, and fresh culture medium (containing 1% fetal bovine serum) was added. The blank control group received no intervention, while the sample groups received culture medium containing the test substance. Locations with uniform scratch thickness and cell density were selected, their coordinates recorded, and photographs taken at different time points to compare the healing speed of each group. The percentage of healed scratch area in each group was measured using software.

[0147] 4.3 Result Calculation

[0148] Calculation of the scratch adhesion rate of the test substance:

[0149]

[0150] In the formula: C n —Scratch area at nH; C0—Scratch area at 0H.

[0151] Example 1 was tested as the test substance according to the above method, and the results are shown in Table 6 and... Figure 1-9 As shown;

[0152] in Figure 1 This is a cell scratch image of the blank control group at 0h. Figure 2 This is a cell scratch image of the blank control group at 24 hours. Figure 3 Cell scratch images of the blank control group after 48 hours; Figure 4 This is a cell scratch image of the positive control group at 0h. Figure 5 This is a cell scratch image of the positive control group at 24 hours. Figure 6 Cell scratch images of the positive control group at 48 hours; Figure 7 These are cell scratch images taken at 0h in Example 1. Figure 8 These are cell scratch images from Example 1 group at 24 hours. Figure 9 This is a cell scratch photograph taken 48 hours after Example 1.

[0153] Table 6 Results of skin repair efficacy of the composition in Example 1

[0154]

[0155] According to the results in Table 6:

[0156] The composition of this application has excellent skin repair effects, achieving a cell scratch healing rate of 67% at 24 hours and 91% at 48 hours.

[0157] The above embodiments are preferred embodiments of this application, but the implementation of this application is not limited to the above embodiments. Any changes, modifications, substitutions, combinations, or simplifications made without departing from the spirit and principle of this application shall be considered equivalent substitutions and shall be included within the protection scope of this application.

Claims

1. An anti-wrinkle and soothing composition, characterized in that, The composition comprises, by weight parts, the following components: 15-20 parts hydroxypropyl tetrahydropyranotriol, 10-15 parts tetrahydromethylpyrimidine carboxylic acid, 5-10 parts leukocyte extract, 5-20 parts Edelweiss callus extract solution, and 35-65 parts Bifida ferment filtrate.

2. The anti-wrinkle and soothing composition according to claim 1, characterized in that, The solution of Edelweiss callus extract contains 20 wt% Edelweiss callus extract.

3. Use of the anti-wrinkle and soothing composition as described in any one of claims 1 or 2 as a cosmetic active ingredient.

4. A cosmetic product, characterized in that, The cosmetic contains 1-10 wt% The anti-wrinkle and soothing composition as described in any one of claims 1 or 2.

5. The cosmetic product according to claim 4, characterized in that, The cosmetic product is one of the following: skin care water, serum, lotion, cream, mask, base makeup, or hair care product.

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