Urothelium carcinoma methylation marker kit

Abstract

The invention relates to a methylation marker kit for urinary tract epithelial carcinoma, and further relates to a reagent capable of detecting the methylation state of a DNA (deoxyribonucleic acid) area in which a target gene in a sample is located and application of the reagent in preparation of the urinary tract epithelial carcinoma detection kit, and the target gene comprises HOXA9, VIM, SIM2, ZIC4, ZNF154, KCNMA1, FOXF2, TRPS1, RAB37, SEPT9, LHX8, NKX6-2, ZNF571-AS1, NKX1-1, MARCH11, PEK, TBX15, NKX2-8, NRN1, BCL11B, ALX1, KCNQ1DN, LRRC9, BNC1, IRX4, SLC26A4 The invention also provides a method for detecting urothelial carcinoma.

Description

[0001] This application is a divisional application of Chinese patent application No. 202410585603.6, filed on May 13, 2024, entitled "Urothelial Carcinoma Methylation Marker Reagent Kit". Technical Field

[0002] This application relates to the field of biomedicine, specifically to a biomarker, method, and kit for detecting urothelial carcinoma. Background Technology

[0003] Among numerous cancers, the incidence of urinary system tumors is increasing year by year, thus further expanding the demand for precision diagnosis and treatment of urinary system tumors. Urothelial carcinoma (UC) includes upper urinary tract urothelial carcinoma (UTUC) and lower urinary tract urothelial carcinoma (mainly bladder cancer, BC), characterized by high incidence, multiple primary sites, and a high recurrence rate after surgery. Clinically, commonly used methods for bladder cancer examination include cystoscopy (which allows for biopsy of suspected lesions), urine cytology, imaging examinations, and fluorescence in situ hybridization.

[0004] Clinically, the diagnosis and post-treatment monitoring of urothelial carcinoma (UC) primarily rely on imaging, urine tests, endoscopy, and biopsy. Traditional imaging examinations are difficult to use for small or atypical lesions, leading to challenges in localization and qualitative diagnosis. Urine tests are widely used as an important diagnostic tool in clinical practice, but they all have certain limitations. For example, urine cytology and FISH tests have relatively low sensitivity and specificity and cannot replace endoscopy and biopsy. Endoscopy (ureteroscopy / cystoscopy) combined with biopsy is the gold standard for the diagnosis and follow-up of urothelial tumors, but due to its invasiveness and relatively high cost, patient compliance is poor, making it difficult to detect early tumors and tumor recurrence in some patients, thus missing the opportunity for treatment.

[0005] In clinical practice, the early diagnosis and monitoring of ulcerative bladder cancer (UC) remain unsatisfactory. For example, the accuracy rate of CT scans in detecting muscle-invasive bladder cancer (MIBC) is only 54.9%; urine cytology and fluorescence in situ hybridization (FISH) have low sensitivity and are easily affected by urinary tract infections, bladder inflammation, stones, and hematuria; cystoscopy is the gold standard for diagnosing bladder cancer, but its invasive nature leads to patient resistance, making adherence to this method difficult to guarantee, and carcinoma in situ is not easily detected under cystoscopy and is easily confused with inflammatory changes in the bladder, requiring biopsy for definitive diagnosis.

[0006] Early diagnosis of upper urinary tract urothelial carcinoma (UTUC) remains highly challenging. When UTUC is strongly suspected but imaging studies are inconclusive, ureteroscopy allows direct visualization of the tumor and enables biopsy. However, the location and angle of the tumor can affect the endoscopic examination, often preventing the use of a rigid ureteroscope to reach the tumor site and thus hindering diagnosis. Ureteral obstruction can also affect endoscopic results, making it difficult to determine the cause of obstruction. Causes of obstruction include ureteral stones, ureteropelvic junction stenosis, and ureteral malignancies. Furthermore, ureteroscopy itself presents certain challenges. Besides the inherent risks of trauma and infection associated with ureteroscopy, the likelihood of postoperative exudation and adhesions in surrounding tissues significantly increases, complicating radical surgery and making it unsuitable for routine procedures.

[0007] Currently, although some genes that are abnormally expressed in patients with urinary system tumors are known, such as FGFR3, PKM2, CCNE1, and PIK3CA, not all abnormal genes can be used as diagnostic markers and cannot meet the clinical requirements for detection performance such as sensitivity and creativity.

[0008] Therefore, it is necessary to provide a non-invasive, highly sensitive, and specific product for accurate early screening and prognosis of urothelial carcinoma. Summary of the Invention

[0009] To improve the sensitivity and specificity of early diagnosis of urothelial carcinoma, and to achieve accurate early diagnosis and recurrence monitoring, this application provides a method for confirming the presence of urothelial carcinoma, assessing the risk of urothelial carcinoma occurrence, and / or assessing the prognosis / progress of urothelial carcinoma, as well as a corresponding detection kit and / or detection model.

[0010] This application is the first to discover the correlation between the methylation of the target gene and urothelial carcinoma, and applies it to the detection of urothelial carcinoma. The target gene of this application can be used alone or in combination with other urothelial carcinoma detection biomarkers for the development of detection products.

[0011] This application uses specially selected biomarkers and methylated gene fragments as detection indicators for the early diagnosis and prognosis assessment of urothelial carcinoma. Firstly, the detection method uses urinary gDNA as a template for initial sample testing, making it a non-invasive method that effectively avoids complications such as urogenital infections, urethral and bladder bleeding, urethral injury, and urethral stricture. It is also easy to perform and can be done at home. Secondly, conventional urinary cytology testing has insufficient sensitivity and a risk of false negatives, especially for G1 grade (well-differentiated papillary carcinoma) and low-grade tumors, where the sensitivity is only 16%. In contrast, the detection method in this application detects biomarkers including HOXA9, VIM, SIM2, ZIC4, ZNF154, KCNMA1, and FOXF2, or HOXA9, TRPS1, RAB37, SEPT9, LHX8, NKX6-2, ZNF571-AS1, NKX1-1, and MARC. Methylation status of genes including H11, PENK, TBX15, NKX2-8, NRN1, BCL11B, ALX1, and KCNQ1DN, or LRRC9, BNC1, IRX4, SLC26A4, and PAX1, effectively utilizes multiplex information within a limited sample to effectively differentiate tumors, especially early-stage tumors, exhibiting high sensitivity and specificity. Furthermore, the detection method of this application provides rapid data results, for example, using quantitative real-time PCR, which facilitates timely subsequent treatment for patients. Compared to other gene markers associated with urothelial carcinoma in the prior art, the method, kit, and / or detection model of this application, which includes specific gene combinations, have higher sensitivity and specificity.

[0012] On the one hand, this application provides the use of a reagent capable of detecting the methylation status of a DNA region containing a target gene in a sample in the preparation of a urothelial carcinoma detection kit, wherein the target gene comprises HOXA9.

[0013] On the other hand, this application provides a method for confirming the presence of urothelial carcinoma, assessing the risk of urothelial carcinoma occurrence, and / or assessing the prognosis / progression of urothelial carcinoma, the method comprising detecting the methylation status of a DNA region containing a target gene in a sample, the target gene comprising HOXA9.

[0014] In some embodiments, the DNA region of the HOXA9 gene is derived from the region defined in human chr7:27203916-27207001. In some embodiments, the DNA region of the HOXA9 gene includes the region defined in human chr7:27203916-27207001.

[0015] In some embodiments, the DNA region of the HOXA9 gene is derived from a region selected from the group consisting of: the region defined by human chr7:27206851-27207001, the region defined by human chr7:27203926-27204076, the region defined by human chr7:27205754-27205904, and the region defined by human chr7:27206632-27206782.

[0016] In some embodiments, the HOXA9 gene DNA region includes the region defined by human chr7:27206851-27207001. In some embodiments, the HOXA9 gene DNA region includes the region defined by human chr7:27203926-27204076. In some embodiments, the HOXA9 gene DNA region includes the region defined by human chr7:27205754-27205904. In some embodiments, the HOXA9 gene DNA region includes the region defined by human chr7:27206632-27206782.

[0017] In some implementations, the samples are from cancer patients, suspected cancer patients, cancer-susceptible populations, high-risk cancer populations, or healthy individuals.

[0018] In some implementations, the tumor patient includes patients who have not received anticancer therapy and / or patients who have received anticancer therapy.

[0019] In some embodiments, the sample comprises tissue, cells, and / or body fluids. In some embodiments, the sample is selected from blood, serum, plasma, urine, saliva, sweat, sputum, semen, mucus, tears, lymph, amniotic fluid, interstitial fluid, bronchoalveolar lavage fluid, cerebrospinal fluid, feces, and tissue samples.

[0020] In some embodiments, the sample contains blood. In some embodiments, the sample contains urine.

[0021] In some embodiments, the target gene comprises HOXA9, and also comprises one or more genes selected from the group consisting of: VIM, KCNQ1DN, SIM2, ZNF571-AS1, NKX1-1, MARCH11, ZIC4, BNC1, IRX4, SLC26A4, TBX15, NKX2-8, NRN1, ZNF154, TRPS1, RAB37, SEPT9, LRRC9, PAX1, KCNMA1, FOXF2, LHX8, NKX6-2, PENK, BCL11B, and ALX1.

[0022] In some embodiments, the target gene comprises HOXA9 and further comprises any one of the following genes selected from the group consisting of: VIM, KCNQ1DN, SIM2, ZNF571-AS1, NKX1-1, MARCH11, ZIC4, BNC1, IRX4, SLC26A4, TBX15, NKX2-8, NRN1, ZNF154, TRPS1, RAB37, SEPT9, LRRC9, PAX1, KCNMA1, FOXF2, LHX8, NKX6-2, PENK, BCL11B, and ALX1. In some embodiments, the target gene further comprises KCNMA1. In some embodiments, the target gene further comprises KCNQ1DN. In some embodiments, the target gene further comprises FOXF2. In some embodiments, the target gene further comprises TRPS1. In some embodiments, the target gene further comprises RAB37. In some embodiments, the target gene further comprises LRRC9. In some embodiments, the target gene further comprises BNC1. In some embodiments, the target gene further comprises SEPT9. In some embodiments, the target gene further comprises LHX8. In some embodiments, the target gene further comprises NKX6-2. In some embodiments, the target gene further comprises ZNF571-AS1. In some embodiments, the target gene further comprises VIM. In some embodiments, the target gene further comprises PAX1. In some embodiments, the target gene further comprises SIM2. In some embodiments, the target gene further comprises PENK. In some embodiments, the target gene further comprises TBX15. In some embodiments, the target gene further comprises NKX2-8. In some embodiments, the target gene further comprises NRN1. In some embodiments, the target gene further comprises ZNF154. In some embodiments, the target gene further comprises IRX4. In some embodiments, the target gene further comprises SLC26A4. In some embodiments, the target gene further comprises ZIC4. In some embodiments, the target gene further comprises NKX1-1. In some embodiments, the target gene further comprises MARCH11. In some embodiments, the target gene further comprises BCL11B. In some embodiments, the target gene further comprises ALX1.

[0023] In some embodiments, the target gene comprises HOXA9 and further comprises any two genes selected from the group consisting of: VIM, KCNQ1DN, SIM2, ZNF571-AS1, NKX1-1, MARCH11, ZIC4, BNC1, IRX4, SLC26A4, TBX15, NKX2-8, NRN1, ZNF154, TRPS1, RAB37, SEPT9, LRRC9, PAX1, KCNMA1, FOXF2, LHX8, NKX6-2, PENK, BCL11B, and ALX1. In some embodiments, the target gene comprises HOXA9, VIM, and KCNQ1DN. In some embodiments, the target gene comprises HOXA9, VIM, and SIM2. In some embodiments, the target gene comprises HOXA9, VIM, and ZNF571-AS1. In some embodiments, the target gene comprises HOXA9, VIM, and NKX1-1. In some embodiments, the target gene comprises HOXA9, VIM, and MARCH11. In some embodiments, the target gene comprises HOXA9, VIM, and ZIC4. In some embodiments, the target gene comprises HOXA9, VIM, and BNC1. In some embodiments, the target gene comprises HOXA9, VIM, and IRX4. In some embodiments, the target gene comprises HOXA9, VIM, and SLC26A4. In some embodiments, the target gene comprises HOXA9, VIM, and TBX15. In some embodiments, the target gene comprises HOXA9, VIM, and NKX2-8. In some embodiments, the target gene comprises HOXA9, VIM, and NRN1. In some embodiments, the target gene comprises HOXA9, VIM, and ZNF154. In some embodiments, the target gene comprises HOXA9, VIM, and TRPS1. In some embodiments, the target gene comprises HOXA9, VIM, and RAB37. In some embodiments, the target gene comprises HOXA9, VIM, and SEPT9. In some embodiments, the target gene comprises HOXA9, VIM, and LRRC9. In some embodiments, the target gene comprises HOXA9, VIM, and PAX1. In some embodiments, the target gene comprises HOXA9, VIM, and KCNMA1. In some embodiments, the target gene comprises HOXA9, VIM, and FOXF2. In some embodiments, the target gene comprises HOXA9, VIM, and LHX8. In some embodiments, the target gene comprises HOXA9, VIM, and NKX6-2. In some embodiments, the target gene comprises HOXA9, VIM, and PENK. In some embodiments, the target gene comprises HOXA9, VIM, and BCL11B.In some embodiments, the target gene comprises HOXA9, VIM, and ALX1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, and SIM2. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, and ZNF571-AS1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, and NKX1-1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, and MARCH11. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, and ZIC4. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, and BNC1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, and IRX4. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, and SLC26A4. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, and TBX15. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, and NKX2-8. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, and NRN1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, and ZNF154. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, and TRPS1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, and RAB37. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, and SEPT9. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, and LRRC9. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, and PAX1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, and KCNMA1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, and FOXF2. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, and LHX8. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, and NKX6-2. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, and PENK. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, and BCL11B. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, and ALX1. In some embodiments, the target gene comprises HOXA9, SIM2, and ZNF571-AS1. In some embodiments, the target gene comprises HOXA9, SIM2, and NKX1-1.In some embodiments, the target gene comprises HOXA9, SIM2, and MARCH11. In some embodiments, the target gene comprises HOXA9, SIM2, and ZIC4. In some embodiments, the target gene comprises HOXA9, SIM2, and BNC1. In some embodiments, the target gene comprises HOXA9, SIM2, and IRX4. In some embodiments, the target gene comprises HOXA9, SIM2, and SLC26A4. In some embodiments, the target gene comprises HOXA9, SIM2, and TBX15. In some embodiments, the target gene comprises HOXA9, SIM2, and NKX2-8. In some embodiments, the target gene comprises HOXA9, SIM2, and NRN1. In some embodiments, the target gene comprises HOXA9, SIM2, and ZNF154. In some embodiments, the target gene comprises HOXA9, SIM2, and TRPS1. In some embodiments, the target gene comprises HOXA9, SIM2, and RAB37. In some embodiments, the target gene comprises HOXA9, SIM2, and SEPT9. In some embodiments, the target gene comprises HOXA9, SIM2, and LRRC9. In some embodiments, the target gene comprises HOXA9, SIM2, and PAX1. In some embodiments, the target gene comprises HOXA9, SIM2, and KCNMA1. In some embodiments, the target gene comprises HOXA9, SIM2, and FOXF2. In some embodiments, the target gene comprises HOXA9, SIM2, and LHX8. In some embodiments, the target gene comprises HOXA9, SIM2, and NKX6-2. In some embodiments, the target gene comprises HOXA9, SIM2, and PENK. In some embodiments, the target gene comprises HOXA9, SIM2, and BCL11B. In some embodiments, the target gene comprises HOXA9, SIM2, and ALX1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, and NKX1-1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, and MARCH11. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, and ZIC4. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, and BNC1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, and IRX4. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, and SLC26A4. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, and TBX15.In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, and NKX2-8. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, and NRN1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, and ZNF154. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, and TRPS1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, and RAB37. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, and SEPT9. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, and LRRC9. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, and PAX1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, and FOXF2. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, and LHX8. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, and PENK. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, and BCL11B. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, and ALX1. In some embodiments, the target gene comprises HOXA9, NKX1-1, and MARCH11. In some embodiments, the target gene comprises HOXA9, NKX1-1, and ZIC4. In some embodiments, the target gene comprises HOXA9, NKX1-1, and BNC1. In some embodiments, the target gene comprises HOXA9, NKX1-1, and IRX4. In some embodiments, the target gene comprises HOXA9, NKX1-1, and SLC26A4. In some embodiments, the target gene comprises HOXA9, NKX1-1, and TBX15. In some embodiments, the target gene comprises HOXA9, NKX1-1, and NKX2-8. In some embodiments, the target gene comprises HOXA9, NKX1-1, and NRN1. In some embodiments, the target gene comprises HOXA9, NKX1-1, and ZNF154. In some embodiments, the target gene comprises HOXA9, NKX1-1, and TRPS1. In some embodiments, the target gene comprises HOXA9, NKX1-1, and RAB37.In some embodiments, the target gene comprises HOXA9, NKX1-1, and SEPT9. In some embodiments, the target gene comprises HOXA9, NKX1-1, and LRRC9. In some embodiments, the target gene comprises HOXA9, NKX1-1, and PAX1. In some embodiments, the target gene comprises HOXA9, NKX1-1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, NKX1-1, and FOXF2. In some embodiments, the target gene comprises HOXA9, NKX1-1, and LHX8. In some embodiments, the target gene comprises HOXA9, NKX1-1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, NKX1-1, and PENK. In some embodiments, the target gene comprises HOXA9, NKX1-1, and BCL11B. In some embodiments, the target gene comprises HOXA9, NKX1-1, and ALX1. In some embodiments, the target gene comprises HOXA9, MARCH11, and ZIC4. In some embodiments, the target gene comprises HOXA9, MARCH11, and BNC1. In some embodiments, the target gene comprises HOXA9, MARCH11, and IRX4. In some embodiments, the target gene comprises HOXA9, MARCH11, and SLC26A4. In some embodiments, the target gene comprises HOXA9, MARCH11, and TBX15. In some embodiments, the target gene comprises HOXA9, MARCH11, and NKX2-8. In some embodiments, the target gene comprises HOXA9, MARCH11, and NRN1. In some embodiments, the target gene comprises HOXA9, MARCH11, and ZNF154. In some embodiments, the target gene comprises HOXA9, MARCH11, and TRPS1. In some embodiments, the target gene comprises HOXA9, MARCH11, and RAB37. In some embodiments, the target gene comprises HOXA9, MARCH11, and SEPT9. In some embodiments, the target gene comprises HOXA9, MARCH11, and LRRC9. In some embodiments, the target gene comprises HOXA9, MARCH11, and PAX1. In some embodiments, the target gene comprises HOXA9, MARCH11, and KCNMA1. In some embodiments, the target gene comprises HOXA9, MARCH11, and FOXF2. In some embodiments, the target gene comprises HOXA9, MARCH11, and LHX8. In some embodiments, the target gene comprises HOXA9, MARCH11, and NKX6-2.In some embodiments, the target gene comprises HOXA9, MARCH11, and PENK. In some embodiments, the target gene comprises HOXA9, MARCH11, and BCL11B. In some embodiments, the target gene comprises HOXA9, MARCH11, and ALX1. In some embodiments, the target gene comprises HOXA9, ZIC4, and BNC1. In some embodiments, the target gene comprises HOXA9, ZIC4, and IRX4. In some embodiments, the target gene comprises HOXA9, ZIC4, and SLC26A4. In some embodiments, the target gene comprises HOXA9, ZIC4, and TBX15. In some embodiments, the target gene comprises HOXA9, ZIC4, and NKX2-8. In some embodiments, the target gene comprises HOXA9, ZIC4, and NRN1. In some embodiments, the target gene comprises HOXA9, ZIC4, and ZNF154. In some embodiments, the target gene comprises HOXA9, ZIC4, and TRPS1. In some embodiments, the target gene comprises HOXA9, ZIC4, and RAB37. In some embodiments, the target gene comprises HOXA9, ZIC4, and SEPT9. In some embodiments, the target gene comprises HOXA9, ZIC4, and LRRC9. In some embodiments, the target gene comprises HOXA9, ZIC4, and PAX1. In some embodiments, the target gene comprises HOXA9, ZIC4, and KCNMA1. In some embodiments, the target gene comprises HOXA9, ZIC4, and FOXF2. In some embodiments, the target gene comprises HOXA9, ZIC4, and LHX8. In some embodiments, the target gene comprises HOXA9, ZIC4, and NKX6-2. In some embodiments, the target gene comprises HOXA9, ZIC4, and PENK. In some embodiments, the target gene comprises HOXA9, ZIC4, and BCL11B. In some embodiments, the target gene comprises HOXA9, ZIC4, and ALX1. In some embodiments, the target gene comprises HOXA9, BNC1, and IRX4. In some embodiments, the target gene comprises HOXA9, BNC1, and SLC26A4. In some embodiments, the target gene comprises HOXA9, BNC1, and TBX15. In some embodiments, the target gene comprises HOXA9, BNC1, and NKX2-8. In some embodiments, the target gene comprises HOXA9, BNC1, and NRN1. In some embodiments, the target gene comprises HOXA9, BNC1, and ZNF154. In some embodiments, the target gene comprises HOXA9, BNC1, and TRPS1.In some embodiments, the target gene comprises HOXA9, BNC1, and RAB37. In some embodiments, the target gene comprises HOXA9, BNC1, and SEPT9. In some embodiments, the target gene comprises HOXA9, BNC1, and LRRC9. In some embodiments, the target gene comprises HOXA9, BNC1, and PAX1. In some embodiments, the target gene comprises HOXA9, BNC1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, BNC1, and FOXF2. In some embodiments, the target gene comprises HOXA9, BNC1, and LHX8. In some embodiments, the target gene comprises HOXA9, BNC1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, BNC1, and PENK. In some embodiments, the target gene comprises HOXA9, BNC1, and BCL11B. In some embodiments, the target gene comprises HOXA9, BNC1, and ALX1. In some embodiments, the target gene comprises HOXA9, IRX4, and SLC26A4. In some embodiments, the target gene comprises HOXA9, IRX4, and TBX15. In some embodiments, the target gene comprises HOXA9, IRX4, and NKX2-8. In some embodiments, the target gene comprises HOXA9, IRX4, and NRN1. In some embodiments, the target gene comprises HOXA9, IRX4, and ZNF154. In some embodiments, the target gene comprises HOXA9, IRX4, and TRPS1. In some embodiments, the target gene comprises HOXA9, IRX4, and RAB37. In some embodiments, the target gene comprises HOXA9, IRX4, and SEPT9. In some embodiments, the target gene comprises HOXA9, IRX4, and LRRC9. In some embodiments, the target gene comprises HOXA9, IRX4, and PAX1. In some embodiments, the target gene comprises HOXA9, IRX4, and KCNMA1. In some embodiments, the target gene comprises HOXA9, IRX4, and FOXF2. In some embodiments, the target gene comprises HOXA9, IRX4, and LHX8. In some embodiments, the target gene comprises HOXA9, IRX4, and NKX6-2. In some embodiments, the target gene comprises HOXA9, IRX4, and PENK. In some embodiments, the target gene comprises HOXA9, IRX4, and BCL11B. In some embodiments, the target gene comprises HOXA9, IRX4, and ALX1. In some embodiments, the target gene comprises HOXA9, SLC26A4, and TBX15.In some embodiments, the target gene comprises HOXA9, SLC26A4, and NKX2-8. In some embodiments, the target gene comprises HOXA9, SLC26A4, and NRN1. In some embodiments, the target gene comprises HOXA9, SLC26A4, and ZNF154. In some embodiments, the target gene comprises HOXA9, SLC26A4, and TRPS1. In some embodiments, the target gene comprises HOXA9, SLC26A4, and RAB37. In some embodiments, the target gene comprises HOXA9, SLC26A4, and SEPT9. In some embodiments, the target gene comprises HOXA9, SLC26A4, and LRRC9. In some embodiments, the target gene comprises HOXA9, SLC26A4, and PAX1. In some embodiments, the target gene comprises HOXA9, SLC26A4, and KCNMA1. In some embodiments, the target gene comprises HOXA9, SLC26A4, and FOXF2. In some embodiments, the target gene comprises HOXA9, SLC26A4, and LHX8. In some embodiments, the target gene comprises HOXA9, SLC26A4, and NKX6-2. In some embodiments, the target gene comprises HOXA9, SLC26A4, and PENK. In some embodiments, the target gene comprises HOXA9, SLC26A4, and BCL11B. In some embodiments, the target gene comprises HOXA9, SLC26A4, and ALX1. In some embodiments, the target gene comprises HOXA9, TBX15, and NKX2-8. In some embodiments, the target gene comprises HOXA9, TBX15, and NRN1. In some embodiments, the target gene comprises HOXA9, TBX15, and ZNF154. In some embodiments, the target gene comprises HOXA9, TBX15, and TRPS1. In some embodiments, the target gene comprises HOXA9, TBX15, and RAB37. In some embodiments, the target gene comprises HOXA9, TBX15, and SEPT9. In some embodiments, the target gene comprises HOXA9, TBX15, and LRRC9. In some embodiments, the target gene comprises HOXA9, TBX15, and PAX1. In some embodiments, the target gene comprises HOXA9, TBX15, and KCNMA1. In some embodiments, the target gene comprises HOXA9, TBX15, and FOXF2. In some embodiments, the target gene comprises HOXA9, TBX15, and LHX8. In some embodiments, the target gene comprises HOXA9, TBX15, and NKX6-2.In some embodiments, the target gene comprises HOXA9, TBX15, and PENK. In some embodiments, the target gene comprises HOXA9, TBX15, and BCL11B. In some embodiments, the target gene comprises HOXA9, TBX15, and ALX1. In some embodiments, the target gene comprises HOXA9, NKX2-8, and NRN1. In some embodiments, the target gene comprises HOXA9, NKX2-8, and ZNF154. In some embodiments, the target gene comprises HOXA9, NKX2-8, and TRPS1. In some embodiments, the target gene comprises HOXA9, NKX2-8, and RAB37. In some embodiments, the target gene comprises HOXA9, NKX2-8, and SEPT9. In some embodiments, the target gene comprises HOXA9, NKX2-8, and LRRC9. In some embodiments, the target gene comprises HOXA9, NKX2-8, and PAX1. In some embodiments, the target gene comprises HOXA9, NKX2-8, and KCNMA1. In some embodiments, the target gene comprises HOXA9, NKX2-8, and FOXF2. In some embodiments, the target gene comprises HOXA9, NKX2-8, and LHX8. In some embodiments, the target gene comprises HOXA9, NKX2-8, and NKX6-2. In some embodiments, the target gene comprises HOXA9, NKX2-8, and PENK. In some embodiments, the target gene comprises HOXA9, NKX2-8, and BCL11B. In some embodiments, the target gene comprises HOXA9, NKX2-8, and ALX1. In some embodiments, the target gene comprises HOXA9, NRN1, and ZNF154. In some embodiments, the target gene comprises HOXA9, NRN1, and TRPS1. In some embodiments, the target gene comprises HOXA9, NRN1, and RAB37. In some embodiments, the target gene comprises HOXA9, NRN1, and SEPT9. In some embodiments, the target gene comprises HOXA9, NRN1, and LRRC9. In some embodiments, the target gene comprises HOXA9, NRN1, and PAX1. In some embodiments, the target gene comprises HOXA9, NRN1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, NRN1, and FOXF2. In some embodiments, the target gene comprises HOXA9, NRN1, and LHX8. In some embodiments, the target gene comprises HOXA9, NRN1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, NRN1, and PENK.In some embodiments, the target gene comprises HOXA9, NRN1, and BCL11B. In some embodiments, the target gene comprises HOXA9, NRN1, and ALX1. In some embodiments, the target gene comprises HOXA9, ZNF154, and TRPS1. In some embodiments, the target gene comprises HOXA9, ZNF154, and RAB37. In some embodiments, the target gene comprises HOXA9, ZNF154, and SEPT9. In some embodiments, the target gene comprises HOXA9, ZNF154, and LRRC9. In some embodiments, the target gene comprises HOXA9, ZNF154, and PAX1. In some embodiments, the target gene comprises HOXA9, ZNF154, and KCNMA1. In some embodiments, the target gene comprises HOXA9, ZNF154, and FOXF2. In some embodiments, the target gene comprises HOXA9, ZNF154, and LHX8. In some embodiments, the target gene comprises HOXA9, ZNF154, and NKX6-2. In some embodiments, the target gene comprises HOXA9, ZNF154, and PENK. In some embodiments, the target gene comprises HOXA9, ZNF154, and BCL11B. In some embodiments, the target gene comprises HOXA9, ZNF154, and ALX1. In some embodiments, the target gene comprises HOXA9, TRPS1, and RAB37. In some embodiments, the target gene comprises HOXA9, TRPS1, and SEPT9. In some embodiments, the target gene comprises HOXA9, TRPS1, and LRRC9. In some embodiments, the target gene comprises HOXA9, TRPS1, and PAX1. In some embodiments, the target gene comprises HOXA9, TRPS1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, TRPS1, and FOXF2. In some embodiments, the target gene comprises HOXA9, TRPS1, and LHX8. In some embodiments, the target gene comprises HOXA9, TRPS1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, TRPS1, and PENK. In some embodiments, the target gene comprises HOXA9, TRPS1, and BCL11B. In some embodiments, the target gene comprises HOXA9, TRPS1, and ALX1. In some embodiments, the target gene comprises HOXA9, RAB37, and SEPT9. In some embodiments, the target gene comprises HOXA9, RAB37, and LRRC9. In some embodiments, the target gene comprises HOXA9, RAB37, and PAX1.In some embodiments, the target gene comprises HOXA9, RAB37, and KCNMA1. In some embodiments, the target gene comprises HOXA9, RAB37, and FOXF2. In some embodiments, the target gene comprises HOXA9, RAB37, and LHX8. In some embodiments, the target gene comprises HOXA9, RAB37, and NKX6-2. In some embodiments, the target gene comprises HOXA9, RAB37, and PENK. In some embodiments, the target gene comprises HOXA9, RAB37, and BCL11B. In some embodiments, the target gene comprises HOXA9, RAB37, and ALX1. In some embodiments, the target gene comprises HOXA9, SEPT9, and LRRC9. In some embodiments, the target gene comprises HOXA9, SEPT9, and PAX1. In some embodiments, the target gene comprises HOXA9, SEPT9, and KCNMA1. In some embodiments, the target gene comprises HOXA9, SEPT9, and FOXF2. In some embodiments, the target gene comprises HOXA9, SEPT9, and LHX8. In some embodiments, the target gene comprises HOXA9, SEPT9, and NKX6-2. In some embodiments, the target gene comprises HOXA9, SEPT9, and PENK. In some embodiments, the target gene comprises HOXA9, SEPT9, and BCL11B. In some embodiments, the target gene comprises HOXA9, SEPT9, and ALX1. In some embodiments, the target gene comprises HOXA9, LRRC9, and PAX1. In some embodiments, the target gene comprises HOXA9, LRRC9, and KCNMA1. In some embodiments, the target gene comprises HOXA9, LRRC9, and FOXF2. In some embodiments, the target gene comprises HOXA9, LRRC9, and LHX8. In some embodiments, the target gene comprises HOXA9, LRRC9, and NKX6-2. In some embodiments, the target gene comprises HOXA9, LRRC9, and PENK. In some embodiments, the target gene comprises HOXA9, LRRC9, and BCL11B. In some embodiments, the target gene comprises HOXA9, LRRC9, and ALX1. In some embodiments, the target gene comprises HOXA9, PAX1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, PAX1, and FOXF2. In some embodiments, the target gene comprises HOXA9, PAX1, and LHX8. In some embodiments, the target gene comprises HOXA9, PAX1, and NKX6-2.In some embodiments, the target gene comprises HOXA9, PAX1, and PENK. In some embodiments, the target gene comprises HOXA9, PAX1, and BCL11B. In some embodiments, the target gene comprises HOXA9, PAX1, and ALX1. In some embodiments, the target gene comprises HOXA9, KCNMA1, and FOXF2. In some embodiments, the target gene comprises HOXA9, KCNMA1, and LHX8. In some embodiments, the target gene comprises HOXA9, KCNMA1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, KCNMA1, and PENK. In some embodiments, the target gene comprises HOXA9, KCNMA1, and BCL11B. In some embodiments, the target gene comprises HOXA9, KCNMA1, and ALX1. In some embodiments, the target gene comprises HOXA9, FOXF2, and LHX8. In some embodiments, the target gene comprises HOXA9, FOXF2, and NKX6-2. In some embodiments, the target gene comprises HOXA9, FOXF2, and PENK. In some embodiments, the target gene comprises HOXA9, FOXF2, and BCL11B. In some embodiments, the target gene comprises HOXA9, FOXF2, and ALX1. In some embodiments, the target gene comprises HOXA9, LHX8, and NKX6-2. In some embodiments, the target gene comprises HOXA9, LHX8, and PENK. In some embodiments, the target gene comprises HOXA9, LHX8, and BCL11B. In some embodiments, the target gene comprises HOXA9, LHX8, and ALX1. In some embodiments, the target gene comprises HOXA9, NKX6-2, and PENK. In some embodiments, the target gene comprises HOXA9, NKX6-2, and BCL11B. In some embodiments, the target gene comprises HOXA9, NKX6-2, and ALX1. In some embodiments, the target gene comprises HOXA9, PENK, and BCL11B. In some embodiments, the target gene comprises HOXA9, PENK, and ALX1. In some embodiments, the target gene comprises HOXA9, BCL11B, and ALX1.

[0024] In some embodiments, the target gene comprises HOXA9, and also comprises any three genes selected from the group consisting of: VIM, KCNQ1DN, SIM2, ZNF571-AS1, NKX1-1, MARCH11, ZIC4, BNC1, IRX4, SLC26A4, TBX15, NKX2-8, NRN1, ZNF154, TRPS1, RAB37, SEPT9, LRRC9, PAX1, KCNMA1, FOXF2, LHX8, NKX6-2, PENK, BCL11B, and ALX1.

[0025] In some embodiments, the target gene comprises HOXA9, VIM, KCNQ1DN, and SIM2. In some embodiments, the target gene comprises HOXA9, VIM, KCNQ1DN, and ZNF571-AS1. In some embodiments, the target gene comprises HOXA9, VIM, KCNQ1DN, and NKX1-1. In some embodiments, the target gene comprises HOXA9, VIM, KCNQ1DN, and MARCH11. In some embodiments, the target gene comprises HOXA9, VIM, KCNQ1DN, and ZIC4. In some embodiments, the target gene comprises HOXA9, VIM, KCNQ1DN, and BNC1. In some embodiments, the target gene comprises HOXA9, VIM, KCNQ1DN, and IRX4. In some embodiments, the target gene comprises HOXA9, VIM, KCNQ1DN, and SLC26A4. In some embodiments, the target gene comprises HOXA9, VIM, KCNQ1DN, and TBX15. In some embodiments, the target gene comprises HOXA9, VIM, KCNQ1DN, and NKX2-8. In some embodiments, the target gene comprises HOXA9, VIM, KCNQ1DN, and NRN1. In some embodiments, the target gene comprises HOXA9, VIM, KCNQ1DN, and ZNF154. In some embodiments, the target gene comprises HOXA9, VIM, KCNQ1DN, and TRPS1. In some embodiments, the target gene comprises HOXA9, VIM, KCNQ1DN, and RAB37. In some embodiments, the target gene comprises HOXA9, VIM, KCNQ1DN, and SEPT9. In some embodiments, the target gene comprises HOXA9, VIM, KCNQ1DN, and LRRC9. In some embodiments, the target gene comprises HOXA9, VIM, KCNQ1DN, and PAX1. In some embodiments, the target gene comprises HOXA9, VIM, KCNQ1DN, and KCNMA1. In some embodiments, the target gene comprises HOXA9, VIM, KCNQ1DN, and FOXF2. In some embodiments, the target gene comprises HOXA9, VIM, KCNQ1DN, and LHX8. In some embodiments, the target gene comprises HOXA9, VIM, KCNQ1DN, and NKX6-2. In some embodiments, the target gene comprises HOXA9, VIM, KCNQ1DN, and PENK. In some embodiments, the target gene comprises HOXA9, VIM, KCNQ1DN, and BCL11B. In some embodiments, the target gene comprises HOXA9, VIM, KCNQ1DN, and ALX1.In some embodiments, the target gene comprises HOXA9, VIM, SIM2, and ZNF571-AS1. In some embodiments, the target gene comprises HOXA9, VIM, SIM2, and NKX1-1. In some embodiments, the target gene comprises HOXA9, VIM, SIM2, and MARCH11. In some embodiments, the target gene comprises HOXA9, VIM, SIM2, and ZIC4. In some embodiments, the target gene comprises HOXA9, VIM, SIM2, and BNC1. In some embodiments, the target gene comprises HOXA9, VIM, SIM2, and IRX4. In some embodiments, the target gene comprises HOXA9, VIM, SIM2, and SLC26A4. In some embodiments, the target gene comprises HOXA9, VIM, SIM2, and TBX15. In some embodiments, the target gene comprises HOXA9, VIM, SIM2, and NKX2-8. In some embodiments, the target gene comprises HOXA9, VIM, SIM2, and NRN1. In some embodiments, the target gene comprises HOXA9, VIM, SIM2, and ZNF154. In some embodiments, the target gene comprises HOXA9, VIM, SIM2, and TRPS1. In some embodiments, the target gene comprises HOXA9, VIM, SIM2, and RAB37. In some embodiments, the target gene comprises HOXA9, VIM, SIM2, and SEPT9. In some embodiments, the target gene comprises HOXA9, VIM, SIM2, and LRRC9. In some embodiments, the target gene comprises HOXA9, VIM, SIM2, and PAX1. In some embodiments, the target gene comprises HOXA9, VIM, SIM2, and KCNMA1. In some embodiments, the target gene comprises HOXA9, VIM, SIM2, and FOXF2. In some embodiments, the target gene comprises HOXA9, VIM, SIM2, and LHX8. In some embodiments, the target gene comprises HOXA9, VIM, SIM2, and NKX6-2. In some embodiments, the target gene comprises HOXA9, VIM, SIM2, and PENK. In some embodiments, the target gene comprises HOXA9, VIM, SIM2, and BCL11B. In some embodiments, the target gene comprises HOXA9, VIM, SIM2, and ALX1. In some embodiments, the target gene comprises HOXA9, VIM, ZNF571-AS1, and NKX1-1. In some embodiments, the target gene comprises HOXA9, VIM, ZNF571-AS1, and MARCH11.In some embodiments, the target gene comprises HOXA9, VIM, ZNF571-AS1, and ZIC4. In some embodiments, the target gene comprises HOXA9, VIM, ZNF571-AS1, and BNC1. In some embodiments, the target gene comprises HOXA9, VIM, ZNF571-AS1, and IRX4. In some embodiments, the target gene comprises HOXA9, VIM, ZNF571-AS1, and SLC26A4. In some embodiments, the target gene comprises HOXA9, VIM, ZNF571-AS1, and TBX15. In some embodiments, the target gene comprises HOXA9, VIM, ZNF571-AS1, and NKX2-8. In some embodiments, the target gene comprises HOXA9, VIM, ZNF571-AS1, and NRN1. In some embodiments, the target gene comprises HOXA9, VIM, ZNF571-AS1, and ZNF154. In some embodiments, the target gene comprises HOXA9, VIM, ZNF571-AS1, and TRPS1. In some embodiments, the target gene comprises HOXA9, VIM, ZNF571-AS1, and RAB37. In some embodiments, the target gene comprises HOXA9, VIM, ZNF571-AS1, and SEPT9. In some embodiments, the target gene comprises HOXA9, VIM, ZNF571-AS1, and LRRC9. In some embodiments, the target gene comprises HOXA9, VIM, ZNF571-AS1, and PAX1. In some embodiments, the target gene comprises HOXA9, VIM, ZNF571-AS1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, VIM, ZNF571-AS1, and FOXF2. In some embodiments, the target gene comprises HOXA9, VIM, ZNF571-AS1, and LHX8. In some embodiments, the target gene comprises HOXA9, VIM, ZNF571-AS1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, VIM, ZNF571-AS1, and PENK. In some embodiments, the target gene comprises HOXA9, VIM, ZNF571-AS1, and BCL11B. In some embodiments, the target gene comprises HOXA9, VIM, ZNF571-AS1, and ALX1. In some embodiments, the target gene comprises HOXA9, VIM, NKX1-1, and MARCH11. In some embodiments, the target gene comprises HOXA9, VIM, NKX1-1, and ZIC4. In some embodiments, the target gene comprises HOXA9, VIM, NKX1-1, and BNC1.In some embodiments, the target gene comprises HOXA9, VIM, NKX1-1, and IRX4. In some embodiments, the target gene comprises HOXA9, VIM, NKX1-1, and SLC26A4. In some embodiments, the target gene comprises HOXA9, VIM, NKX1-1, and TBX15. In some embodiments, the target gene comprises HOXA9, VIM, NKX1-1, and NKX2-8. In some embodiments, the target gene comprises HOXA9, VIM, NKX1-1, and NRN1. In some embodiments, the target gene comprises HOXA9, VIM, NKX1-1, and ZNF154. In some embodiments, the target gene comprises HOXA9, VIM, NKX1-1, and TRPS1. In some embodiments, the target gene comprises HOXA9, VIM, NKX1-1, and RAB37. In some embodiments, the target gene comprises HOXA9, VIM, NKX1-1, and SEPT9. In some embodiments, the target gene comprises HOXA9, VIM, NKX1-1, and LRRC9. In some embodiments, the target gene comprises HOXA9, VIM, NKX1-1, and PAX1. In some embodiments, the target gene comprises HOXA9, VIM, NKX1-1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, VIM, NKX1-1, and FOXF2. In some embodiments, the target gene comprises HOXA9, VIM, NKX1-1, and LHX8. In some embodiments, the target gene comprises HOXA9, VIM, NKX1-1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, VIM, NKX1-1, and PENK. In some embodiments, the target gene comprises HOXA9, VIM, NKX1-1, and BCL11B. In some embodiments, the target gene comprises HOXA9, VIM, NKX1-1, and ALX1. In some embodiments, the target gene comprises HOXA9, VIM, MARCH11, and ZIC4. In some embodiments, the target gene comprises HOXA9, VIM, MARCH11, and BNC1. In some embodiments, the target gene comprises HOXA9, VIM, MARCH11, and IRX4. In some embodiments, the target gene comprises HOXA9, VIM, MARCH11, and SLC26A4. In some embodiments, the target gene comprises HOXA9, VIM, MARCH11, and TBX15. In some embodiments, the target gene comprises HOXA9, VIM, MARCH11, and NKX2-8.In some embodiments, the target gene comprises HOXA9, VIM, MARCH11, and NRN1. In some embodiments, the target gene comprises HOXA9, VIM, MARCH11, and ZNF154. In some embodiments, the target gene comprises HOXA9, VIM, MARCH11, and TRPS1. In some embodiments, the target gene comprises HOXA9, VIM, MARCH11, and RAB37. In some embodiments, the target gene comprises HOXA9, VIM, MARCH11, and SEPT9. In some embodiments, the target gene comprises HOXA9, VIM, MARCH11, and LRRC9. In some embodiments, the target gene comprises HOXA9, VIM, MARCH11, and PAX1. In some embodiments, the target gene comprises HOXA9, VIM, MARCH11, and KCNMA1. In some embodiments, the target gene comprises HOXA9, VIM, MARCH11, and FOXF2. In some embodiments, the target gene comprises HOXA9, VIM, MARCH11, and LHX8. In some embodiments, the target gene comprises HOXA9, VIM, MARCH11, and NKX6-2. In some embodiments, the target gene comprises HOXA9, VIM, MARCH11, and PENK. In some embodiments, the target gene comprises HOXA9, VIM, MARCH11, and BCL11B. In some embodiments, the target gene comprises HOXA9, VIM, MARCH11, and ALX1. In some embodiments, the target gene comprises HOXA9, VIM, ZIC4, and BNC1. In some embodiments, the target gene comprises HOXA9, VIM, ZIC4, and IRX4. In some embodiments, the target gene comprises HOXA9, VIM, ZIC4, and SLC26A4. In some embodiments, the target gene comprises HOXA9, VIM, ZIC4, and TBX15. In some embodiments, the target gene comprises HOXA9, VIM, ZIC4, and NKX2-8. In some embodiments, the target gene comprises HOXA9, VIM, ZIC4, and NRN1. In some embodiments, the target gene comprises HOXA9, VIM, ZIC4, and ZNF154. In some embodiments, the target gene comprises HOXA9, VIM, ZIC4, and TRPS1. In some embodiments, the target gene comprises HOXA9, VIM, ZIC4, and RAB37. In some embodiments, the target gene comprises HOXA9, VIM, ZIC4, and SEPT9. In some embodiments, the target gene comprises HOXA9, VIM, ZIC4, and LRRC9.In some embodiments, the target gene comprises HOXA9, VIM, ZIC4, and PAX1. In some embodiments, the target gene comprises HOXA9, VIM, ZIC4, and KCNMA1. In some embodiments, the target gene comprises HOXA9, VIM, ZIC4, and FOXF2. In some embodiments, the target gene comprises HOXA9, VIM, ZIC4, and LHX8. In some embodiments, the target gene comprises HOXA9, VIM, ZIC4, and NKX6-2. In some embodiments, the target gene comprises HOXA9, VIM, ZIC4, and PENK. In some embodiments, the target gene comprises HOXA9, VIM, ZIC4, and BCL11B. In some embodiments, the target gene comprises HOXA9, VIM, ZIC4, and ALX1. In some embodiments, the target gene comprises HOXA9, VIM, BNC1, and IRX4. In some embodiments, the target gene comprises HOXA9, VIM, BNC1, and SLC26A4. In some embodiments, the target gene comprises HOXA9, VIM, BNC1, and TBX15. In some embodiments, the target gene comprises HOXA9, VIM, BNC1, and NKX2-8. In some embodiments, the target gene comprises HOXA9, VIM, BNC1, and NRN1. In some embodiments, the target gene comprises HOXA9, VIM, BNC1, and ZNF154. In some embodiments, the target gene comprises HOXA9, VIM, BNC1, and TRPS1. In some embodiments, the target gene comprises HOXA9, VIM, BNC1, and RAB37. In some embodiments, the target gene comprises HOXA9, VIM, BNC1, and SEPT9. In some embodiments, the target gene comprises HOXA9, VIM, BNC1, and LRRC9. In some embodiments, the target gene comprises HOXA9, VIM, BNC1, and PAX1. In some embodiments, the target gene comprises HOXA9, VIM, BNC1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, VIM, BNC1, and FOXF2. In some embodiments, the target gene comprises HOXA9, VIM, BNC1, and LHX8. In some embodiments, the target gene comprises HOXA9, VIM, BNC1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, VIM, BNC1, and PENK. In some embodiments, the target gene comprises HOXA9, VIM, BNC1, and BCL11B. In some embodiments, the target gene comprises HOXA9, VIM, BNC1, and ALX1.In some embodiments, the target gene comprises HOXA9, VIM, IRX4, and SLC26A4. In some embodiments, the target gene comprises HOXA9, VIM, IRX4, and TBX15. In some embodiments, the target gene comprises HOXA9, VIM, IRX4, and NKX2-8. In some embodiments, the target gene comprises HOXA9, VIM, IRX4, and NRN1. In some embodiments, the target gene comprises HOXA9, VIM, IRX4, and ZNF154. In some embodiments, the target gene comprises HOXA9, VIM, IRX4, and TRPS1. In some embodiments, the target gene comprises HOXA9, VIM, IRX4, and RAB37. In some embodiments, the target gene comprises HOXA9, VIM, IRX4, and SEPT9. In some embodiments, the target gene comprises HOXA9, VIM, IRX4, and LRRC9. In some embodiments, the target gene comprises HOXA9, VIM, IRX4, and PAX1. In some embodiments, the target gene comprises HOXA9, VIM, IRX4, and KCNMA1. In some embodiments, the target gene comprises HOXA9, VIM, IRX4, and FOXF2. In some embodiments, the target gene comprises HOXA9, VIM, IRX4, and LHX8. In some embodiments, the target gene comprises HOXA9, VIM, IRX4, and NKX6-2. In some embodiments, the target gene comprises HOXA9, VIM, IRX4, and PENK. In some embodiments, the target gene comprises HOXA9, VIM, IRX4, and BCL11B. In some embodiments, the target gene comprises HOXA9, VIM, IRX4, and ALX1. In some embodiments, the target gene comprises HOXA9, VIM, SLC26A4, and TBX15. In some embodiments, the target gene comprises HOXA9, VIM, SLC26A4, and NKX2-8. In some embodiments, the target gene comprises HOXA9, VIM, SLC26A4, and NRN1. In some embodiments, the target gene comprises HOXA9, VIM, SLC26A4, and ZNF154. In some embodiments, the target gene comprises HOXA9, VIM, SLC26A4, and TRPS1. In some embodiments, the target gene comprises HOXA9, VIM, SLC26A4, and RAB37. In some embodiments, the target gene comprises HOXA9, VIM, SLC26A4, and SEPT9. In some embodiments, the target gene comprises HOXA9, VIM, SLC26A4, and LRRC9.In some embodiments, the target gene comprises HOXA9, VIM, SLC26A4, and PAX1. In some embodiments, the target gene comprises HOXA9, VIM, SLC26A4, and KCNMA1. In some embodiments, the target gene comprises HOXA9, VIM, SLC26A4, and FOXF2. In some embodiments, the target gene comprises HOXA9, VIM, SLC26A4, and LHX8. In some embodiments, the target gene comprises HOXA9, VIM, SLC26A4, and NKX6-2. In some embodiments, the target gene comprises HOXA9, VIM, SLC26A4, and PENK. In some embodiments, the target gene comprises HOXA9, VIM, SLC26A4, and BCL11B. In some embodiments, the target gene comprises HOXA9, VIM, SLC26A4, and ALX1. In some embodiments, the target gene comprises HOXA9, VIM, TBX15, and NKX2-8. In some embodiments, the target gene comprises HOXA9, VIM, TBX15, and NRN1. In some embodiments, the target gene comprises HOXA9, VIM, TBX15, and ZNF154. In some embodiments, the target gene comprises HOXA9, VIM, TBX15, and TRPS1. In some embodiments, the target gene comprises HOXA9, VIM, TBX15, and RAB37. In some embodiments, the target gene comprises HOXA9, VIM, TBX15, and SEPT9. In some embodiments, the target gene comprises HOXA9, VIM, TBX15, and LRRC9. In some embodiments, the target gene comprises HOXA9, VIM, TBX15, and PAX1. In some embodiments, the target gene comprises HOXA9, VIM, TBX15, and KCNMA1. In some embodiments, the target gene comprises HOXA9, VIM, TBX15, and FOXF2. In some embodiments, the target gene comprises HOXA9, VIM, TBX15, and LHX8. In some embodiments, the target gene comprises HOXA9, VIM, TBX15, and NKX6-2. In some embodiments, the target gene comprises HOXA9, VIM, TBX15, and PENK. In some embodiments, the target gene comprises HOXA9, VIM, TBX15, and BCL11B. In some embodiments, the target gene comprises HOXA9, VIM, TBX15, and ALX1. In some embodiments, the target gene comprises HOXA9, VIM, NKX2-8, and NRN1. In some embodiments, the target gene comprises HOXA9, VIM, NKX2-8, and ZNF154.In some embodiments, the target gene comprises HOXA9, VIM, NKX2-8, and TRPS1. In some embodiments, the target gene comprises HOXA9, VIM, NKX2-8, and RAB37. In some embodiments, the target gene comprises HOXA9, VIM, NKX2-8, and SEPT9. In some embodiments, the target gene comprises HOXA9, VIM, NKX2-8, and LRRC9. In some embodiments, the target gene comprises HOXA9, VIM, NKX2-8, and PAX1. In some embodiments, the target gene comprises HOXA9, VIM, NKX2-8, and KCNMA1. In some embodiments, the target gene comprises HOXA9, VIM, NKX2-8, and FOXF2. In some embodiments, the target gene comprises HOXA9, VIM, NKX2-8, and LHX8. In some embodiments, the target gene comprises HOXA9, VIM, NKX2-8, and NKX6-2. In some embodiments, the target gene comprises HOXA9, VIM, NKX2-8, and PENK. In some embodiments, the target gene comprises HOXA9, VIM, NKX2-8, and BCL11B. In some embodiments, the target gene comprises HOXA9, VIM, NKX2-8, and ALX1. In some embodiments, the target gene comprises HOXA9, VIM, NRN1, and ZNF154. In some embodiments, the target gene comprises HOXA9, VIM, NRN1, and TRPS1. In some embodiments, the target gene comprises HOXA9, VIM, NRN1, and RAB37. In some embodiments, the target gene comprises HOXA9, VIM, NRN1, and SEPT9. In some embodiments, the target gene comprises HOXA9, VIM, NRN1, and LRRC9. In some embodiments, the target gene comprises HOXA9, VIM, NRN1, and PAX1. In some embodiments, the target gene comprises HOXA9, VIM, NRN1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, VIM, NRN1, and FOXF2. In some embodiments, the target gene comprises HOXA9, VIM, NRN1, and LHX8. In some embodiments, the target gene comprises HOXA9, VIM, NRN1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, VIM, NRN1, and PENK. In some embodiments, the target gene comprises HOXA9, VIM, NRN1, and BCL11B. In some embodiments, the target gene comprises HOXA9, VIM, NRN1, and ALX1.In some embodiments, the target gene comprises HOXA9, VIM, ZNF154, and TRPS1. In some embodiments, the target gene comprises HOXA9, VIM, ZNF154, and RAB37. In some embodiments, the target gene comprises HOXA9, VIM, ZNF154, and SEPT9. In some embodiments, the target gene comprises HOXA9, VIM, ZNF154, and LRRC9. In some embodiments, the target gene comprises HOXA9, VIM, ZNF154, and PAX1. In some embodiments, the target gene comprises HOXA9, VIM, ZNF154, and KCNMA1. In some embodiments, the target gene comprises HOXA9, VIM, ZNF154, and FOXF2. In some embodiments, the target gene comprises HOXA9, VIM, ZNF154, and LHX8. In some embodiments, the target gene comprises HOXA9, VIM, ZNF154, and NKX6-2. In some embodiments, the target gene comprises HOXA9, VIM, ZNF154, and PENK. In some embodiments, the target gene comprises HOXA9, VIM, ZNF154, and BCL11B. In some embodiments, the target gene comprises HOXA9, VIM, ZNF154, and ALX1. In some embodiments, the target gene comprises HOXA9, VIM, TRPS1, and RAB37. In some embodiments, the target gene comprises HOXA9, VIM, TRPS1, and SEPT9. In some embodiments, the target gene comprises HOXA9, VIM, TRPS1, and LRRC9. In some embodiments, the target gene comprises HOXA9, VIM, TRPS1, and PAX1. In some embodiments, the target gene comprises HOXA9, VIM, TRPS1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, VIM, TRPS1, and FOXF2. In some embodiments, the target gene comprises HOXA9, VIM, TRPS1, and LHX8. In some embodiments, the target gene comprises HOXA9, VIM, TRPS1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, VIM, TRPS1, and PENK. In some embodiments, the target gene comprises HOXA9, VIM, TRPS1, and BCL11B. In some embodiments, the target gene comprises HOXA9, VIM, TRPS1, and ALX1. In some embodiments, the target gene comprises HOXA9, VIM, RAB37, and SEPT9. In some embodiments, the target gene comprises HOXA9, VIM, RAB37, and LRRC9.In some embodiments, the target gene comprises HOXA9, VIM, RAB37, and PAX1. In some embodiments, the target gene comprises HOXA9, VIM, RAB37, and KCNMA1. In some embodiments, the target gene comprises HOXA9, VIM, RAB37, and FOXF2. In some embodiments, the target gene comprises HOXA9, VIM, RAB37, and LHX8. In some embodiments, the target gene comprises HOXA9, VIM, RAB37, and NKX6-2. In some embodiments, the target gene comprises HOXA9, VIM, RAB37, and PENK. In some embodiments, the target gene comprises HOXA9, VIM, RAB37, and BCL11B. In some embodiments, the target gene comprises HOXA9, VIM, RAB37, and ALX1. In some embodiments, the target gene comprises HOXA9, VIM, SEPT9, and LRRC9. In some embodiments, the target gene comprises HOXA9, VIM, SEPT9, and PAX1. In some embodiments, the target gene comprises HOXA9, VIM, SEPT9, and KCNMA1. In some embodiments, the target gene comprises HOXA9, VIM, SEPT9, and FOXF2. In some embodiments, the target gene comprises HOXA9, VIM, SEPT9, and LHX8. In some embodiments, the target gene comprises HOXA9, VIM, SEPT9, and NKX6-2. In some embodiments, the target gene comprises HOXA9, VIM, SEPT9, and PENK. In some embodiments, the target gene comprises HOXA9, VIM, SEPT9, and BCL11B. In some embodiments, the target gene comprises HOXA9, VIM, SEPT9, and ALX1. In some embodiments, the target gene comprises HOXA9, VIM, LRRC9, and PAX1. In some embodiments, the target gene comprises HOXA9, VIM, LRRC9, and KCNMA1. In some embodiments, the target gene comprises HOXA9, VIM, LRRC9, and FOXF2. In some embodiments, the target gene comprises HOXA9, VIM, LRRC9, and LHX8. In some embodiments, the target gene comprises HOXA9, VIM, LRRC9, and NKX6-2. In some embodiments, the target gene comprises HOXA9, VIM, LRRC9, and PENK. In some embodiments, the target gene comprises HOXA9, VIM, LRRC9, and BCL11B. In some embodiments, the target gene comprises HOXA9, VIM, LRRC9, and ALX1.In some embodiments, the target gene comprises HOXA9, VIM, PAX1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, VIM, PAX1, and FOXF2. In some embodiments, the target gene comprises HOXA9, VIM, PAX1, and LHX8. In some embodiments, the target gene comprises HOXA9, VIM, PAX1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, VIM, PAX1, and PENK. In some embodiments, the target gene comprises HOXA9, VIM, PAX1, and BCL11B. In some embodiments, the target gene comprises HOXA9, VIM, PAX1, and ALX1. In some embodiments, the target gene comprises HOXA9, VIM, KCNMA1, and FOXF2. In some embodiments, the target gene comprises HOXA9, VIM, KCNMA1, and LHX8. In some embodiments, the target gene comprises HOXA9, VIM, KCNMA1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, VIM, KCNMA1, and PENK. In some embodiments, the target gene comprises HOXA9, VIM, KCNMA1, and BCL11B. In some embodiments, the target gene comprises HOXA9, VIM, KCNMA1, and ALX1. In some embodiments, the target gene comprises HOXA9, VIM, FOXF2, and LHX8. In some embodiments, the target gene comprises HOXA9, VIM, FOXF2, and NKX6-2. In some embodiments, the target gene comprises HOXA9, VIM, FOXF2, and PENK. In some embodiments, the target gene comprises HOXA9, VIM, FOXF2, and BCL11B. In some embodiments, the target gene comprises HOXA9, VIM, FOXF2, and ALX1. In some embodiments, the target gene comprises HOXA9, VIM, LHX8, and NKX6-2. In some embodiments, the target gene comprises HOXA9, VIM, LHX8, and PENK. In some embodiments, the target gene comprises HOXA9, VIM, LHX8, and BCL11B. In some embodiments, the target gene comprises HOXA9, VIM, LHX8, and ALX1. In some embodiments, the target gene comprises HOXA9, VIM, NKX6-2, and PENK. In some embodiments, the target gene comprises HOXA9, VIM, NKX6-2, and BCL11B. In some embodiments, the target gene comprises HOXA9, VIM, NKX6-2, and ALX1.In some embodiments, the target gene comprises HOXA9, VIM, PENK, and BCL11B. In some embodiments, the target gene comprises HOXA9, VIM, PENK, and ALX1. In some embodiments, the target gene comprises HOXA9, VIM, BCL11B, and ALX1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, SIM2, and ZNF571-AS1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, SIM2, and NKX1-1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, SIM2, and MARCH11. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, SIM2, and ZIC4. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, SIM2, and BNC1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, SIM2, and IRX4. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, SIM2, and SLC26A4. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, SIM2, and TBX15. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, SIM2, and NKX2-8. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, SIM2, and NRN1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, SIM2, and ZNF154. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, SIM2, and TRPS1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, SIM2, and RAB37. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, SIM2, and SEPT9. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, SIM2, and LRRC9. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, SIM2, and PAX1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, SIM2, and KCNMA1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, SIM2, and FOXF2. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, SIM2, and LHX8. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, SIM2, and NKX6-2.In some embodiments, the target gene comprises HOXA9, KCNQ1DN, SIM2, and PENK. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, SIM2, and BCL11B. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, SIM2, and ALX1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, ZNF571-AS1, and NKX1-1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, ZNF571-AS1, and MARCH11. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, ZNF571-AS1, and ZIC4. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, ZNF571-AS1, and BNC1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, ZNF571-AS1, and IRX4. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, ZNF571-AS1, and SLC26A4. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, ZNF571-AS1, and TBX15. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, ZNF571-AS1, and NKX2-8. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, ZNF571-AS1, and NRN1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, ZNF571-AS1, and ZNF154. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, ZNF571-AS1, and TRPS1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, ZNF571-AS1, and RAB37. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, ZNF571-AS1, and SEPT9. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, ZNF571-AS1, and LRRC9. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, ZNF571-AS1, and PAX1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, ZNF571-AS1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, ZNF571-AS1, and FOXF2. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, ZNF571-AS1, and LHX8.In some embodiments, the target gene comprises HOXA9, KCNQ1DN, ZNF571-AS1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, ZNF571-AS1, and PENK. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, ZNF571-AS1, and BCL11B. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, NKX1-1, and MARCH11. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, NKX1-1, and ZIC4. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, NKX1-1, and BNC1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, NKX1-1, and IRX4. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, NKX1-1, and SLC26A4. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, NKX1-1, and TBX15. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, NKX1-1, and NKX2-8. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, NKX1-1, and NRN1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, NKX1-1, and ZNF154. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, NKX1-1, and TRPS1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, NKX1-1, and RAB37. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, NKX1-1, and SEPT9. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, NKX1-1, and LRRC9. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, NKX1-1, and PAX1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, NKX1-1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, NKX1-1, and FOXF2. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, NKX1-1, and LHX8. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, NKX1-1, and NKX6-2.In some embodiments, the target gene comprises HOXA9, KCNQ1DN, NKX1-1, and PENK. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, NKX1-1, and BCL11B. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, NKX1-1, and ALX1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, MARCH11, and ZIC4. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, MARCH11, and BNC1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, MARCH11, and IRX4. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, MARCH11, and SLC26A4. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, MARCH11, and TBX15. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, MARCH11, and NKX2-8. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, MARCH11, and NRN1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, MARCH11, and ZNF154. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, MARCH11, and TRPS1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, MARCH11, and RAB37. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, MARCH11, and SEPT9. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, MARCH11, and LRRC9. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, MARCH11, and PAX1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, MARCH11, and KCNMA1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, MARCH11, and FOXF2. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, MARCH11, and LHX8. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, MARCH11, and NKX6-2. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, MARCH11, and PENK. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, MARCH11, and BCL11B.In some embodiments, the target gene comprises HOXA9, KCNQ1DN, MARCH11, and ALX1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, ZIC4, and BNC1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, ZIC4, and IRX4. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, ZIC4, and SLC26A4. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, ZIC4, and TBX15. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, ZIC4, and NKX2-8. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, ZIC4, and NRN1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, ZIC4, and ZNF154. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, ZIC4, and TRPS1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, ZIC4, and RAB37. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, ZIC4, and SEPT9. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, ZIC4, and LRRC9. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, ZIC4, and PAX1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, ZIC4, and KCNMA1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, ZIC4, and FOXF2. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, ZIC4, and LHX8. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, ZIC4, and NKX6-2. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, ZIC4, and PENK. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, ZIC4, and BCL11B. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, ZIC4, and ALX1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, BNC1, and IRX4. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, BNC1, and SLC26A4. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, BNC1, and TBX15.In some embodiments, the target gene comprises HOXA9, KCNQ1DN, BNC1, and NKX2-8. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, BNC1, and NRN1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, BNC1, and ZNF154. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, BNC1, and TRPS1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, BNC1, and RAB37. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, BNC1, and SEPT9. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, BNC1, and LRRC9. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, BNC1, and PAX1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, BNC1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, BNC1, and FOXF2. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, BNC1, and LHX8. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, BNC1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, BNC1, and PENK. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, BNC1, and BCL11B. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, BNC1, and ALX1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, IRX4, and SLC26A4. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, IRX4, and TBX15. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, IRX4, and NKX2-8. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, IRX4, and NRN1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, IRX4, and ZNF154. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, IRX4, and TRPS1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, IRX4, and RAB37. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, IRX4, and SEPT9.In some embodiments, the target gene comprises HOXA9, KCNQ1DN, IRX4, and LRRC9. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, IRX4, and PAX1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, IRX4, and KCNMA1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, IRX4, and FOXF2. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, IRX4, and LHX8. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, IRX4, and NKX6-2. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, IRX4, and PENK. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, IRX4, and BCL11B. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, IRX4, and ALX1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, SLC26A4, and TBX15. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, SLC26A4, and NKX2-8. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, SLC26A4, and NRN1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, SLC26A4, and ZNF154. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, SLC26A4, and TRPS1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, SLC26A4, and RAB37. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, SLC26A4, and SEPT9. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, SLC26A4, and LRRC9. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, SLC26A4, and PAX1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, SLC26A4, and KCNMA1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, SLC26A4, and FOXF2. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, SLC26A4, and LHX8. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, SLC26A4, and NKX6-2.In some embodiments, the target gene comprises HOXA9, KCNQ1DN, SLC26A4, and PENK. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, SLC26A4, and BCL11B. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, SLC26A4, and ALX1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, TBX15, and NKX2-8. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, TBX15, and NRN1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, TBX15, and ZNF154. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, TBX15, and TRPS1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, TBX15, and RAB37. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, TBX15, and SEPT9. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, TBX15, and LRRC9. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, TBX15, and PAX1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, TBX15, and KCNMA1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, TBX15, and FOXF2. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, TBX15, and LHX8. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, TBX15, and NKX6-2. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, TBX15, and PENK. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, TBX15, and BCL11B. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, TBX15, and ALX1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, NKX2-8, and NRN1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, NKX2-8, and ZNF154. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, NKX2-8, and TRPS1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, NKX2-8, and RAB37. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, NKX2-8, and SEPT9.In some embodiments, the target gene comprises HOXA9, KCNQ1DN, NKX2-8, and LRRC9. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, NKX2-8, and PAX1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, NKX2-8, and KCNMA1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, NKX2-8, and FOXF2. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, NKX2-8, and LHX8. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, NKX2-8, and NKX6-2. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, NKX2-8, and PENK. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, NKX2-8, and BCL11B. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, NKX2-8, and ALX1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, NRN1, and ZNF154. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, NRN1, and TRPS1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, NRN1, and RAB37. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, NRN1, and SEPT9. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, NRN1, and LRRC9. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, NRN1, and PAX1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, NRN1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, NRN1, and FOXF2. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, NRN1, and LHX8. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, NRN1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, NRN1, and PENK. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, NRN1, and BCL11B. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, NRN1, and ALX1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, ZNF154, and TRPS1.In some embodiments, the target gene comprises HOXA9, KCNQ1DN, ZNF154, and RAB37. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, ZNF154, and SEPT9. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, ZNF154, and LRRC9. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, ZNF154, and PAX1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, ZNF154, and KCNMA1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, ZNF154, and FOXF2. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, ZNF154, and LHX8. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, ZNF154, and NKX6-2. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, ZNF154, and PENK. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, ZNF154, and BCL11B. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, ZNF154, and ALX1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, TRPS1, and RAB37. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, TRPS1, and SEPT9. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, TRPS1, and LRRC9. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, TRPS1, and PAX1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, TRPS1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, TRPS1, and FOXF2. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, TRPS1, and LHX8. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, TRPS1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, TRPS1, and PENK. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, TRPS1, and BCL11B. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, TRPS1, and ALX1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, RAB37, and SEPT9.In some embodiments, the target gene comprises HOXA9, KCNQ1DN, RAB37, and LRRC9. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, RAB37, and PAX1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, RAB37, and KCNMA1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, RAB37, and FOXF2. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, RAB37, and LHX8. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, RAB37, and NKX6-2. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, RAB37, and PENK. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, RAB37, and BCL11B. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, RAB37, and ALX1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, SEPT9, and LRRC9. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, SEPT9, and PAX1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, SEPT9, and KCNMA1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, SEPT9, and FOXF2. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, SEPT9, and LHX8. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, SEPT9, and NKX6-2. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, SEPT9, and PENK. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, SEPT9, and BCL11B. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, SEPT9, and ALX1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, LRRC9, and PAX1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, LRRC9, and KCNMA1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, LRRC9, and FOXF2. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, LRRC9, and LHX8. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, LRRC9, and NKX6-2.In some embodiments, the target gene comprises HOXA9, KCNQ1DN, LRRC9, and PENK. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, LRRC9, and BCL11B. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, LRRC9, and ALX1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, PAX1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, PAX1, and FOXF2. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, PAX1, and LHX8. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, PAX1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, PAX1, and PENK. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, PAX1, and BCL11B. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, PAX1, and ALX1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, KCNMA1, and FOXF2. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, KCNMA1, and LHX8. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, KCNMA1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, KCNMA1, and PENK. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, KCNMA1, and BCL11B. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, KCNMA1, and ALX1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, FOXF2, and LHX8. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, FOXF2, and NKX6-2. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, FOXF2, and PENK. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, FOXF2, and BCL11B. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, FOXF2, and ALX1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, LHX8, and NKX6-2. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, LHX8, and PENK.In some embodiments, the target gene comprises HOXA9, KCNQ1DN, LHX8, and BCL11B. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, LHX8, and ALX1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, NKX6-2, and PENK. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, NKX6-2, and BCL11B. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, NKX6-2, and ALX1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, PENK, and BCL11B. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, PENK, and ALX1. In some embodiments, the target gene comprises HOXA9, KCNQ1DN, BCL11B, and ALX1. In some embodiments, the target gene comprises HOXA9, SIM2, ZNF571-AS1, and NKX1-1. In some embodiments, the target gene comprises HOXA9, SIM2, ZNF571-AS1, and MARCH11. In some embodiments, the target gene comprises HOXA9, SIM2, ZNF571-AS1, and ZIC4. In some embodiments, the target gene comprises HOXA9, SIM2, ZNF571-AS1, and BNC1. In some embodiments, the target gene comprises HOXA9, SIM2, ZNF571-AS1, and IRX4. In some embodiments, the target gene comprises HOXA9, SIM2, ZNF571-AS1, and SLC26A4. In some embodiments, the target gene comprises HOXA9, SIM2, ZNF571-AS1, and TBX15. In some embodiments, the target gene comprises HOXA9, SIM2, ZNF571-AS1, and NKX2-8. In some embodiments, the target gene comprises HOXA9, SIM2, ZNF571-AS1, and NRN1. In some embodiments, the target gene comprises HOXA9, SIM2, ZNF571-AS1, and ZNF154. In some embodiments, the target gene comprises HOXA9, SIM2, ZNF571-AS1, and TRPS1. In some embodiments, the target gene comprises HOXA9, SIM2, ZNF571-AS1, and RAB37. In some embodiments, the target gene comprises HOXA9, SIM2, ZNF571-AS1, and SEPT9. In some embodiments, the target gene comprises HOXA9, SIM2, ZNF571-AS1, and LRRC9.In some embodiments, the target gene comprises HOXA9, SIM2, ZNF571-AS1, and PAX1. In some embodiments, the target gene comprises HOXA9, SIM2, ZNF571-AS1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, SIM2, ZNF571-AS1, and FOXF2. In some embodiments, the target gene comprises HOXA9, SIM2, ZNF571-AS1, and LHX8. In some embodiments, the target gene comprises HOXA9, SIM2, ZNF571-AS1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, SIM2, ZNF571-AS1, and PENK. In some embodiments, the target gene comprises HOXA9, SIM2, ZNF571-AS1, and BCL11B. In some embodiments, the target gene comprises HOXA9, SIM2, ZNF571-AS1, and ALX1. In some embodiments, the target gene comprises HOXA9, SIM2, NKX1-1, and MARCH11. In some embodiments, the target gene comprises HOXA9, SIM2, NKX1-1, and ZIC4. In some embodiments, the target gene comprises HOXA9, SIM2, NKX1-1, and BNC1. In some embodiments, the target gene comprises HOXA9, SIM2, NKX1-1, and IRX4. In some embodiments, the target gene comprises HOXA9, SIM2, NKX1-1, and SLC26A4. In some embodiments, the target gene comprises HOXA9, SIM2, NKX1-1, and TBX15. In some embodiments, the target gene comprises HOXA9, SIM2, NKX1-1, and NKX2-8. In some embodiments, the target gene comprises HOXA9, SIM2, NKX1-1, and NRN1. In some embodiments, the target gene comprises HOXA9, SIM2, NKX1-1, and ZNF154. In some embodiments, the target gene comprises HOXA9, SIM2, NKX1-1, and TRPS1. In some embodiments, the target gene comprises HOXA9, SIM2, NKX1-1, and RAB37. In some embodiments, the target gene comprises HOXA9, SIM2, NKX1-1, and SEPT9. In some embodiments, the target gene comprises HOXA9, SIM2, NKX1-1, and LRRC9. In some embodiments, the target gene comprises HOXA9, SIM2, NKX1-1, and PAX1. In some embodiments, the target gene comprises HOXA9, SIM2, NKX1-1, and KCNMA1.In some embodiments, the target gene comprises HOXA9, SIM2, NKX1-1, and FOXF2. In some embodiments, the target gene comprises HOXA9, SIM2, NKX1-1, and LHX8. In some embodiments, the target gene comprises HOXA9, SIM2, NKX1-1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, SIM2, NKX1-1, and PENK. In some embodiments, the target gene comprises HOXA9, SIM2, NKX1-1, and BCL11B. In some embodiments, the target gene comprises HOXA9, SIM2, NKX1-1, and ALX1. In some embodiments, the target gene comprises HOXA9, SIM2, MARCH11, and ZIC4. In some embodiments, the target gene comprises HOXA9, SIM2, MARCH11, and BNC1. In some embodiments, the target gene comprises HOXA9, SIM2, MARCH11, and IRX4. In some embodiments, the target gene comprises HOXA9, SIM2, MARCH11, and SLC26A4. In some embodiments, the target gene comprises HOXA9, SIM2, MARCH11, and TBX15. In some embodiments, the target gene comprises HOXA9, SIM2, MARCH11, and NKX2-8. In some embodiments, the target gene comprises HOXA9, SIM2, MARCH11, and NRN1. In some embodiments, the target gene comprises HOXA9, SIM2, MARCH11, and ZNF154. In some embodiments, the target gene comprises HOXA9, SIM2, MARCH11, and TRPS1. In some embodiments, the target gene comprises HOXA9, SIM2, MARCH11, and RAB37. In some embodiments, the target gene comprises HOXA9, SIM2, MARCH11, and SEPT9. In some embodiments, the target gene comprises HOXA9, SIM2, MARCH11, and LRRC9. In some embodiments, the target gene comprises HOXA9, SIM2, MARCH11, and PAX1. In some embodiments, the target gene comprises HOXA9, SIM2, MARCH11, and KCNMA1. In some embodiments, the target gene comprises HOXA9, SIM2, MARCH11, and FOXF2. In some embodiments, the target gene comprises HOXA9, SIM2, MARCH11, and LHX8. In some embodiments, the target gene comprises HOXA9, SIM2, MARCH11, and NKX6-2. In some embodiments, the target gene comprises HOXA9, SIM2, MARCH11, and PENK.In some embodiments, the target gene comprises HOXA9, SIM2, MARCH11, and BCL11B. In some embodiments, the target gene comprises HOXA9, SIM2, MARCH11, and ALX1. In some embodiments, the target gene comprises HOXA9, SIM2, ZIC4, and BNC1. In some embodiments, the target gene comprises HOXA9, SIM2, ZIC4, and IRX4. In some embodiments, the target gene comprises HOXA9, SIM2, ZIC4, and SLC26A4. In some embodiments, the target gene comprises HOXA9, SIM2, ZIC4, and TBX15. In some embodiments, the target gene comprises HOXA9, SIM2, ZIC4, and NKX2-8. In some embodiments, the target gene comprises HOXA9, SIM2, ZIC4, and NRN1. In some embodiments, the target gene comprises HOXA9, SIM2, ZIC4, and ZNF154. In some embodiments, the target gene comprises HOXA9, SIM2, ZIC4, and TRPS1. In some embodiments, the target gene comprises HOXA9, SIM2, ZIC4, and RAB37. In some embodiments, the target gene comprises HOXA9, SIM2, ZIC4, and SEPT9. In some embodiments, the target gene comprises HOXA9, SIM2, ZIC4, and LRRC9. In some embodiments, the target gene comprises HOXA9, SIM2, ZIC4, and PAX1. In some embodiments, the target gene comprises HOXA9, SIM2, ZIC4, and KCNMA1. In some embodiments, the target gene comprises HOXA9, SIM2, ZIC4, and FOXF2. In some embodiments, the target gene comprises HOXA9, SIM2, ZIC4, and LHX8. In some embodiments, the target gene comprises HOXA9, SIM2, ZIC4, and NKX6-2. In some embodiments, the target gene comprises HOXA9, SIM2, ZIC4, and PENK. In some embodiments, the target gene comprises HOXA9, SIM2, ZIC4, and BCL11B. In some embodiments, the target gene comprises HOXA9, SIM2, ZIC4, and ALX1. In some embodiments, the target gene comprises HOXA9, SIM2, BNC1, and IRX4. In some embodiments, the target gene comprises HOXA9, SIM2, BNC1, and SLC26A4. In some embodiments, the target gene comprises HOXA9, SIM2, BNC1, and TBX15. In some embodiments, the target gene comprises HOXA9, SIM2, BNC1, and NKX2-8.In some embodiments, the target gene comprises HOXA9, SIM2, BNC1, and NRN1. In some embodiments, the target gene comprises HOXA9, SIM2, BNC1, and ZNF154. In some embodiments, the target gene comprises HOXA9, SIM2, BNC1, and TRPS1. In some embodiments, the target gene comprises HOXA9, SIM2, BNC1, and RAB37. In some embodiments, the target gene comprises HOXA9, SIM2, BNC1, and SEPT9. In some embodiments, the target gene comprises HOXA9, SIM2, BNC1, and LRRC9. In some embodiments, the target gene comprises HOXA9, SIM2, BNC1, and PAX1. In some embodiments, the target gene comprises HOXA9, SIM2, BNC1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, SIM2, BNC1, and FOXF2. In some embodiments, the target gene comprises HOXA9, SIM2, BNC1, and LHX8. In some embodiments, the target gene comprises HOXA9, SIM2, BNC1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, SIM2, BNC1, and PENK. In some embodiments, the target gene comprises HOXA9, SIM2, BNC1, and BCL11B. In some embodiments, the target gene comprises HOXA9, SIM2, BNC1, and ALX1. In some embodiments, the target gene comprises HOXA9, SIM2, IRX4, and SLC26A4. In some embodiments, the target gene comprises HOXA9, SIM2, IRX4, and TBX15. In some embodiments, the target gene comprises HOXA9, SIM2, IRX4, and NKX2-8. In some embodiments, the target gene comprises HOXA9, SIM2, IRX4, and NRN1. In some embodiments, the target gene comprises HOXA9, SIM2, IRX4, and ZNF154. In some embodiments, the target gene comprises HOXA9, SIM2, IRX4, and TRPS1. In some embodiments, the target gene comprises HOXA9, SIM2, IRX4, and RAB37. In some embodiments, the target gene comprises HOXA9, SIM2, IRX4, and SEPT9. In some embodiments, the target gene comprises HOXA9, SIM2, IRX4, and LRRC9. In some embodiments, the target gene comprises HOXA9, SIM2, IRX4, and PAX1. In some embodiments, the target gene comprises HOXA9, SIM2, IRX4, and KCNMA1.In some embodiments, the target gene comprises HOXA9, SIM2, IRX4, and FOXF2. In some embodiments, the target gene comprises HOXA9, SIM2, IRX4, and LHX8. In some embodiments, the target gene comprises HOXA9, SIM2, IRX4, and NKX6-2. In some embodiments, the target gene comprises HOXA9, SIM2, IRX4, and PENK. In some embodiments, the target gene comprises HOXA9, SIM2, IRX4, and BCL11B. In some embodiments, the target gene comprises HOXA9, SIM2, IRX4, and ALX1. In some embodiments, the target gene comprises HOXA9, SIM2, SLC26A4, and TBX15. In some embodiments, the target gene comprises HOXA9, SIM2, SLC26A4, and NKX2-8. In some embodiments, the target gene comprises HOXA9, SIM2, SLC26A4, and NRN1. In some embodiments, the target gene comprises HOXA9, SIM2, SLC26A4, and ZNF154. In some embodiments, the target gene comprises HOXA9, SIM2, SLC26A4, and TRPS1. In some embodiments, the target gene comprises HOXA9, SIM2, SLC26A4, and RAB37. In some embodiments, the target gene comprises HOXA9, SIM2, SLC26A4, and SEPT9. In some embodiments, the target gene comprises HOXA9, SIM2, SLC26A4, and LRRC9. In some embodiments, the target gene comprises HOXA9, SIM2, SLC26A4, and PAX1. In some embodiments, the target gene comprises HOXA9, SIM2, SLC26A4, and KCNMA1. In some embodiments, the target gene comprises HOXA9, SIM2, SLC26A4, and FOXF2. In some embodiments, the target gene comprises HOXA9, SIM2, SLC26A4, and LHX8. In some embodiments, the target gene comprises HOXA9, SIM2, SLC26A4, and NKX6-2. In some embodiments, the target gene comprises HOXA9, SIM2, SLC26A4, and PENK. In some embodiments, the target gene comprises HOXA9, SIM2, SLC26A4, and BCL11B. In some embodiments, the target gene comprises HOXA9, SIM2, SLC26A4, and ALX1. In some embodiments, the target gene comprises HOXA9, SIM2, TBX15, and NKX2-8. In some embodiments, the target gene comprises HOXA9, SIM2, TBX15, and NRN1.In some embodiments, the target gene comprises HOXA9, SIM2, TBX15, and ZNF154. In some embodiments, the target gene comprises HOXA9, SIM2, TBX15, and TRPS1. In some embodiments, the target gene comprises HOXA9, SIM2, TBX15, and RAB37. In some embodiments, the target gene comprises HOXA9, SIM2, TBX15, and SEPT9. In some embodiments, the target gene comprises HOXA9, SIM2, TBX15, and LRRC9. In some embodiments, the target gene comprises HOXA9, SIM2, TBX15, and PAX1. In some embodiments, the target gene comprises HOXA9, SIM2, TBX15, and KCNMA1. In some embodiments, the target gene comprises HOXA9, SIM2, TBX15, and FOXF2. In some embodiments, the target gene comprises HOXA9, SIM2, TBX15, and LHX8. In some embodiments, the target gene comprises HOXA9, SIM2, TBX15, and NKX6-2. In some embodiments, the target gene comprises HOXA9, SIM2, TBX15, and PENK. In some embodiments, the target gene comprises HOXA9, SIM2, TBX15, and BCL11B. In some embodiments, the target gene comprises HOXA9, SIM2, TBX15, and ALX1. In some embodiments, the target gene comprises HOXA9, SIM2, NKX2-8, and NRN1. In some embodiments, the target gene comprises HOXA9, SIM2, NKX2-8, and ZNF154. In some embodiments, the target gene comprises HOXA9, SIM2, NKX2-8, and TRPS1. In some embodiments, the target gene comprises HOXA9, SIM2, NKX2-8, and RAB37. In some embodiments, the target gene comprises HOXA9, SIM2, NKX2-8, and SEPT9. In some embodiments, the target gene comprises HOXA9, SIM2, NKX2-8, and LRRC9. In some embodiments, the target gene comprises HOXA9, SIM2, NKX2-8, and PAX1. In some embodiments, the target gene comprises HOXA9, SIM2, NKX2-8, and KCNMA1. In some embodiments, the target gene comprises HOXA9, SIM2, NKX2-8, and FOXF2. In some embodiments, the target gene comprises HOXA9, SIM2, NKX2-8, and LHX8. In some embodiments, the target gene comprises HOXA9, SIM2, NKX2-8, and NKX6-2.In some embodiments, the target gene comprises HOXA9, SIM2, NKX2-8, and PENK. In some embodiments, the target gene comprises HOXA9, SIM2, NKX2-8, and BCL11B. In some embodiments, the target gene comprises HOXA9, SIM2, NKX2-8, and ALX1. In some embodiments, the target gene comprises HOXA9, SIM2, NRN1, and ZNF154. In some embodiments, the target gene comprises HOXA9, SIM2, NRN1, and TRPS1. In some embodiments, the target gene comprises HOXA9, SIM2, NRN1, and RAB37. In some embodiments, the target gene comprises HOXA9, SIM2, NRN1, and SEPT9. In some embodiments, the target gene comprises HOXA9, SIM2, NRN1, and LRRC9. In some embodiments, the target gene comprises HOXA9, SIM2, NRN1, and PAX1. In some embodiments, the target gene comprises HOXA9, SIM2, NRN1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, SIM2, NRN1, and FOXF2. In some embodiments, the target gene comprises HOXA9, SIM2, NRN1, and LHX8. In some embodiments, the target gene comprises HOXA9, SIM2, NRN1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, SIM2, NRN1, and PENK. In some embodiments, the target gene comprises HOXA9, SIM2, NRN1, and BCL11B. In some embodiments, the target gene comprises HOXA9, SIM2, NRN1, and ALX1. In some embodiments, the target gene comprises HOXA9, SIM2, ZNF154, and TRPS1. In some embodiments, the target gene comprises HOXA9, SIM2, ZNF154, and RAB37. In some embodiments, the target gene comprises HOXA9, SIM2, ZNF154, and SEPT9. In some embodiments, the target gene comprises HOXA9, SIM2, ZNF154, and LRRC9. In some embodiments, the target gene comprises HOXA9, SIM2, ZNF154, and PAX1. In some embodiments, the target gene comprises HOXA9, SIM2, ZNF154, and KCNMA1. In some embodiments, the target gene comprises HOXA9, SIM2, ZNF154, and FOXF2. In some embodiments, the target gene comprises HOXA9, SIM2, ZNF154, and LHX8. In some embodiments, the target gene comprises HOXA9, SIM2, ZNF154, and NKX6-2.In some embodiments, the target gene comprises HOXA9, SIM2, ZNF154, and PENK. In some embodiments, the target gene comprises HOXA9, SIM2, ZNF154, and BCL11B. In some embodiments, the target gene comprises HOXA9, SIM2, ZNF154, and ALX1. In some embodiments, the target gene comprises HOXA9, SIM2, TRPS1, and RAB37. In some embodiments, the target gene comprises HOXA9, SIM2, TRPS1, and SEPT9. In some embodiments, the target gene comprises HOXA9, SIM2, TRPS1, and LRRC9. In some embodiments, the target gene comprises HOXA9, SIM2, TRPS1, and PAX1. In some embodiments, the target gene comprises HOXA9, SIM2, TRPS1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, SIM2, TRPS1, and FOXF2. In some embodiments, the target gene comprises HOXA9, SIM2, TRPS1, and LHX8. In some embodiments, the target gene comprises HOXA9, SIM2, TRPS1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, SIM2, TRPS1, and PENK. In some embodiments, the target gene comprises HOXA9, SIM2, TRPS1, and BCL11B. In some embodiments, the target gene comprises HOXA9, SIM2, TRPS1, and ALX1. In some embodiments, the target gene comprises HOXA9, SIM2, RAB37, and SEPT9. In some embodiments, the target gene comprises HOXA9, SIM2, RAB37, and LRRC9. In some embodiments, the target gene comprises HOXA9, SIM2, RAB37, and PAX1. In some embodiments, the target gene comprises HOXA9, SIM2, RAB37, and KCNMA1. In some embodiments, the target gene comprises HOXA9, SIM2, RAB37, and FOXF2. In some embodiments, the target gene comprises HOXA9, SIM2, RAB37, and LHX8. In some embodiments, the target gene comprises HOXA9, SIM2, RAB37, and NKX6-2. In some embodiments, the target gene comprises HOXA9, SIM2, RAB37, and PENK. In some embodiments, the target gene comprises HOXA9, SIM2, RAB37, and BCL11B. In some embodiments, the target gene comprises HOXA9, SIM2, RAB37, and ALX1. In some embodiments, the target gene comprises HOXA9, SIM2, SEPT9, and LRRC9.In some embodiments, the target gene comprises HOXA9, SIM2, SEPT9, and PAX1. In some embodiments, the target gene comprises HOXA9, SIM2, SEPT9, and KCNMA1. In some embodiments, the target gene comprises HOXA9, SIM2, SEPT9, and FOXF2. In some embodiments, the target gene comprises HOXA9, SIM2, SEPT9, and LHX8. In some embodiments, the target gene comprises HOXA9, SIM2, SEPT9, and NKX6-2. In some embodiments, the target gene comprises HOXA9, SIM2, SEPT9, and PENK. In some embodiments, the target gene comprises HOXA9, SIM2, SEPT9, and BCL11B. In some embodiments, the target gene comprises HOXA9, SIM2, SEPT9, and ALX1. In some embodiments, the target gene comprises HOXA9, SIM2, LRRC9, and PAX1. In some embodiments, the target gene comprises HOXA9, SIM2, LRRC9, and KCNMA1. In some embodiments, the target gene comprises HOXA9, SIM2, LRRC9, and FOXF2. In some embodiments, the target gene comprises HOXA9, SIM2, LRRC9, and LHX8. In some embodiments, the target gene comprises HOXA9, SIM2, LRRC9, and NKX6-2. In some embodiments, the target gene comprises HOXA9, SIM2, LRRC9, and PENK. In some embodiments, the target gene comprises HOXA9, SIM2, LRRC9, and BCL11B. In some embodiments, the target gene comprises HOXA9, SIM2, LRRC9, and ALX1. In some embodiments, the target gene comprises HOXA9, SIM2, PAX1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, SIM2, PAX1, and FOXF2. In some embodiments, the target gene comprises HOXA9, SIM2, PAX1, and LHX8. In some embodiments, the target gene comprises HOXA9, SIM2, PAX1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, SIM2, PAX1, and PENK. In some embodiments, the target gene comprises HOXA9, SIM2, PAX1, and BCL11B. In some embodiments, the target gene comprises HOXA9, SIM2, PAX1, and ALX1. In some embodiments, the target gene comprises HOXA9, SIM2, KCNMA1, and FOXF2. In some embodiments, the target gene comprises HOXA9, SIM2, KCNMA1, and LHX8.In some embodiments, the target gene comprises HOXA9, SIM2, KCNMA1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, SIM2, KCNMA1, and PENK. In some embodiments, the target gene comprises HOXA9, SIM2, KCNMA1, and BCL11B. In some embodiments, the target gene comprises HOXA9, SIM2, KCNMA1, and ALX1. In some embodiments, the target gene comprises HOXA9, SIM2, FOXF2, and LHX8. In some embodiments, the target gene comprises HOXA9, SIM2, FOXF2, and NKX6-2. In some embodiments, the target gene comprises HOXA9, SIM2, FOXF2, and PENK. In some embodiments, the target gene comprises HOXA9, SIM2, FOXF2, and BCL11B. In some embodiments, the target gene comprises HOXA9, SIM2, FOXF2, and ALX1. In some embodiments, the target gene comprises HOXA9, SIM2, LHX8, and NKX6-2. In some embodiments, the target gene comprises HOXA9, SIM2, LHX8, and PENK. In some embodiments, the target gene comprises HOXA9, SIM2, LHX8, and BCL11B. In some embodiments, the target gene comprises HOXA9, SIM2, LHX8, and ALX1. In some embodiments, the target gene comprises HOXA9, SIM2, NKX6-2, and PENK. In some embodiments, the target gene comprises HOXA9, SIM2, NKX6-2, and BCL11B. In some embodiments, the target gene comprises HOXA9, SIM2, NKX6-2, and ALX1. In some embodiments, the target gene comprises HOXA9, SIM2, PENK, and BCL11B. In some embodiments, the target gene comprises HOXA9, SIM2, PENK, and ALX1. In some embodiments, the target gene comprises HOXA9, SIM2, BCL11B, and ALX1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, NKX1-1, and MARCH11. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, NKX1-1, and ZIC4. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, NKX1-1, and BNC1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, NKX1-1, and IRX4. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, NKX1-1, and SLC26A4.In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, NKX1-1, and TBX15. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, NKX1-1, and NKX2-8. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, NKX1-1, and NRN1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, NKX1-1, and ZNF154. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, NKX1-1, and TRPS1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, NKX1-1, and RAB37. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, NKX1-1, and SEPT9. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, NKX1-1, and LRRC9. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, NKX1-1, and PAX1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, NKX1-1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, NKX1-1, and FOXF2. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, NKX1-1, and LHX8. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, NKX1-1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, NKX1-1, and PENK. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, NKX1-1, and BCL11B. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, NKX1-1, and ALX1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, MARCH11, and ZIC4. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, MARCH11, and BNC1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, MARCH11, and IRX4. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, MARCH11, and SLC26A4. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, MARCH11, and TBX15.In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, MARCH11, and NKX2-8. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, MARCH11, and NRN1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, MARCH11, and ZNF154. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, MARCH11, and TRPS1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, MARCH11, and RAB37. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, MARCH11, and SEPT9. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, MARCH11, and LRRC9. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, MARCH11, and PAX1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, MARCH11, and KCNMA1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, MARCH11, and FOXF2. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, MARCH11, and LHX8. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, MARCH11, and NKX6-2. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, MARCH11, and PENK. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, MARCH11, and BCL11B. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, MARCH11, and ALX1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, ZIC4, and BNC1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, ZIC4, and IRX4. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, ZIC4, and SLC26A4. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, ZIC4, and TBX15. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, ZIC4, and NKX2-8. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, ZIC4, and NRN1.In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, ZIC4, and ZNF154. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, ZIC4, and TRPS1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, ZIC4, and RAB37. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, ZIC4, and SEPT9. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, ZIC4, and LRRC9. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, ZIC4, and PAX1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, ZIC4, and KCNMA1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, ZIC4, and FOXF2. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, ZIC4, and LHX8. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, ZIC4, and NKX6-2. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, ZIC4, and PENK. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, ZIC4, and BCL11B. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, ZIC4, and ALX1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, BNC1, and IRX4. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, BNC1, and SLC26A4. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, BNC1, and TBX15. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, BNC1, and NKX2-8. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, BNC1, and NRN1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, BNC1, and ZNF154. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, BNC1, and TRPS1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, BNC1, and RAB37. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, BNC1, and SEPT9.In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, BNC1, and LRRC9. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, BNC1, and PAX1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, BNC1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, BNC1, and FOXF2. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, BNC1, and LHX8. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, BNC1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, BNC1, and PENK. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, BNC1, and BCL11B. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, BNC1, and ALX1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, IRX4, and SLC26A4. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, IRX4, and TBX15. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, IRX4, and NKX2-8. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, IRX4, and NRN1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, IRX4, and ZNF154. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, IRX4, and TRPS1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, IRX4, and RAB37. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, IRX4, and SEPT9. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, IRX4, and LRRC9. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, IRX4, and PAX1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, IRX4, and KCNMA1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, IRX4, and FOXF2. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, IRX4, and LHX8.In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, IRX4, and NKX6-2. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, IRX4, and PENK. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, IRX4, and BCL11B. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, IRX4, and ALX1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, SLC26A4, and TBX15. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, SLC26A4, and NKX2-8. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, SLC26A4, and NRN1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, SLC26A4, and ZNF154. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, SLC26A4, and TRPS1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, SLC26A4, and RAB37. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, SLC26A4, and SEPT9. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, SLC26A4, and LRRC9. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, SLC26A4, and PAX1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, SLC26A4, and KCNMA1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, SLC26A4, and FOXF2. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, SLC26A4, and LHX8. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, SLC26A4, and NKX6-2. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, SLC26A4, and PENK. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, SLC26A4, and BCL11B. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, SLC26A4, and ALX1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, TBX15, and NKX2-8.In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, TBX15, and NRN1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, TBX15, and ZNF154. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, TBX15, and TRPS1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, TBX15, and RAB37. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, TBX15, and SEPT9. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, TBX15, and LRRC9. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, TBX15, and PAX1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, TBX15, and KCNMA1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, TBX15, and FOXF2. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, TBX15, and LHX8. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, TBX15, and NKX6-2. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, TBX15, and PENK. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, TBX15, and BCL11B. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, TBX15, and ALX1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, NKX2-8, and NRN1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, NKX2-8, and ZNF154. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, NKX2-8, and TRPS1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, NKX2-8, and RAB37. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, NKX2-8, and SEPT9. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, NKX2-8, and LRRC9. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, NKX2-8, and PAX1.In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, NKX2-8, and KCNMA1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, NKX2-8, and FOXF2. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, NKX2-8, and LHX8. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, NKX2-8, and NKX6-2. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, NKX2-8, and PENK. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, NKX2-8, and BCL11B. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, NKX2-8, and ALX1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, NRN1, and ZNF154. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, NRN1, and TRPS1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, NRN1, and RAB37. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, NRN1, and SEPT9. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, NRN1, and LRRC9. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, NRN1, and PAX1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, NRN1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, NRN1, and FOXF2. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, NRN1, and LHX8. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, NRN1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, NRN1, and PENK. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, NRN1, and BCL11B. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, NRN1, and ALX1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, ZNF154, and TRPS1.In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, ZNF154, and RAB37. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, ZNF154, and SEPT9. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, ZNF154, and LRRC9. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, ZNF154, and PAX1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, ZNF154, and KCNMA1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, ZNF154, and FOXF2. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, ZNF154, and LHX8. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, ZNF154, and NKX6-2. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, ZNF154, and PENK. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, ZNF154, and BCL11B. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, ZNF154, and ALX1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, TRPS1, and RAB37. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, TRPS1, and SEPT9. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, TRPS1, and LRRC9. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, TRPS1, and PAX1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, TRPS1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, TRPS1, and FOXF2. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, TRPS1, and LHX8. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, TRPS1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, TRPS1, and PENK. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, TRPS1, and BCL11B.In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, TRPS1, and ALX1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, RAB37, and SEPT9. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, RAB37, and LRRC9. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, RAB37, and PAX1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, RAB37, and KCNMA1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, RAB37, and FOXF2. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, RAB37, and LHX8. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, RAB37, and NKX6-2. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, RAB37, and PENK. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, RAB37, and BCL11B. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, RAB37, and ALX1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, SEPT9, and LRRC9. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, SEPT9, and PAX1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, SEPT9, and KCNMA1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, SEPT9, and FOXF2. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, SEPT9, and LHX8. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, SEPT9, and NKX6-2. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, SEPT9, and PENK. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, SEPT9, and BCL11B. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, SEPT9, and ALX1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, LRRC9, and PAX1.In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, LRRC9, and KCNMA1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, LRRC9, and FOXF2. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, LRRC9, and LHX8. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, LRRC9, and NKX6-2. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, LRRC9, and PENK. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, LRRC9, and BCL11B. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, LRRC9, and ALX1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, PAX1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, PAX1, and FOXF2. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, PAX1, and LHX8. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, PAX1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, PAX1, and PENK. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, PAX1, and BCL11B. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, PAX1, and ALX1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, KCNMA1, and FOXF2. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, KCNMA1, and LHX8. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, KCNMA1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, KCNMA1, and PENK. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, KCNMA1, and BCL11B. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, KCNMA1, and ALX1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, FOXF2, and LHX8.In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, FOXF2, and NKX6-2. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, FOXF2, and PENK. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, FOXF2, and BCL11B. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, FOXF2, and ALX1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, LHX8, and NKX6-2. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, LHX8, and PENK. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, LHX8, and BCL11B. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, LHX8, and ALX1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, NKX6-2, and PENK. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, NKX6-2, and BCL11B. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, PENK, and BCL11B. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, PENK, and ALX1. In some embodiments, the target gene comprises HOXA9, ZNF571-AS1, BCL11B, and ALX1. In some embodiments, the target gene comprises HOXA9, NKX1-1, MARCH11, and ZIC4. In some embodiments, the target gene comprises HOXA9, NKX1-1, MARCH11, and BNC1. In some embodiments, the target gene comprises HOXA9, NKX1-1, MARCH11, and IRX4. In some embodiments, the target gene comprises HOXA9, NKX1-1, MARCH11, and SLC26A4. In some embodiments, the target gene comprises HOXA9, NKX1-1, MARCH11, and TBX15. In some embodiments, the target gene comprises HOXA9, NKX1-1, MARCH11, and NKX2-8. In some embodiments, the target gene comprises HOXA9, NKX1-1, MARCH11, and NRN1. In some embodiments, the target gene comprises HOXA9, NKX1-1, MARCH11, and ZNF154.In some embodiments, the target gene comprises HOXA9, NKX1-1, MARCH11, and TRPS1. In some embodiments, the target gene comprises HOXA9, NKX1-1, MARCH11, and RAB37. In some embodiments, the target gene comprises HOXA9, NKX1-1, MARCH11, and SEPT9. In some embodiments, the target gene comprises HOXA9, NKX1-1, MARCH11, and LRRC9. In some embodiments, the target gene comprises HOXA9, NKX1-1, MARCH11, and PAX1. In some embodiments, the target gene comprises HOXA9, NKX1-1, MARCH11, and KCNMA1. In some embodiments, the target gene comprises HOXA9, NKX1-1, MARCH11, and FOXF2. In some embodiments, the target gene comprises HOXA9, NKX1-1, MARCH11, and LHX8. In some embodiments, the target gene comprises HOXA9, NKX1-1, MARCH11, and NKX6-2. In some embodiments, the target gene comprises HOXA9, NKX1-1, MARCH11, and PENK. In some embodiments, the target gene comprises HOXA9, NKX1-1, MARCH11, and BCL11B. In some embodiments, the target gene comprises HOXA9, NKX1-1, MARCH11, and ALX1. In some embodiments, the target gene comprises HOXA9, NKX1-1, ZIC4, and BNC1. In some embodiments, the target gene comprises HOXA9, NKX1-1, ZIC4, and IRX4. In some embodiments, the target gene comprises HOXA9, NKX1-1, ZIC4, and SLC26A4. In some embodiments, the target gene comprises HOXA9, NKX1-1, ZIC4, and TBX15. In some embodiments, the target gene comprises HOXA9, NKX1-1, ZIC4, and NKX2-8. In some embodiments, the target gene comprises HOXA9, NKX1-1, ZIC4, and NRN1. In some embodiments, the target gene comprises HOXA9, NKX1-1, ZIC4, and ZNF154. In some embodiments, the target gene comprises HOXA9, NKX1-1, ZIC4, and TRPS1. In some embodiments, the target gene comprises HOXA9, NKX1-1, ZIC4, and RAB37. In some embodiments, the target gene comprises HOXA9, NKX1-1, ZIC4, and SEPT9. In some embodiments, the target gene comprises HOXA9, NKX1-1, ZIC4, and LRRC9.In some embodiments, the target gene comprises HOXA9, NKX1-1, ZIC4, and PAX1. In some embodiments, the target gene comprises HOXA9, NKX1-1, ZIC4, and KCNMA1. In some embodiments, the target gene comprises HOXA9, NKX1-1, ZIC4, and FOXF2. In some embodiments, the target gene comprises HOXA9, NKX1-1, ZIC4, and LHX8. In some embodiments, the target gene comprises HOXA9, NKX1-1, ZIC4, and NKX6-2. In some embodiments, the target gene comprises HOXA9, NKX1-1, ZIC4, and PENK. In some embodiments, the target gene comprises HOXA9, NKX1-1, ZIC4, and BCL11B. In some embodiments, the target gene comprises HOXA9, NKX1-1, ZIC4, and ALX1. In some embodiments, the target gene comprises HOXA9, NKX1-1, BNC1, and IRX4. In some embodiments, the target gene comprises HOXA9, NKX1-1, BNC1, and SLC26A4. In some embodiments, the target gene comprises HOXA9, NKX1-1, BNC1, and TBX15. In some embodiments, the target gene comprises HOXA9, NKX1-1, BNC1, and NKX2-8. In some embodiments, the target gene comprises HOXA9, NKX1-1, BNC1, and NRN1. In some embodiments, the target gene comprises HOXA9, NKX1-1, BNC1, and ZNF154. In some embodiments, the target gene comprises HOXA9, NKX1-1, BNC1, and TRPS1. In some embodiments, the target gene comprises HOXA9, NKX1-1, BNC1, and RAB37. In some embodiments, the target gene comprises HOXA9, NKX1-1, BNC1, and SEPT9. In some embodiments, the target gene comprises HOXA9, NKX1-1, BNC1, and LRRC9. In some embodiments, the target gene comprises HOXA9, NKX1-1, BNC1, and PAX1. In some embodiments, the target gene comprises HOXA9, NKX1-1, BNC1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, NKX1-1, BNC1, and FOXF2. In some embodiments, the target gene comprises HOXA9, NKX1-1, BNC1, and LHX8. In some embodiments, the target gene comprises HOXA9, NKX1-1, BNC1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, NKX1-1, BNC1, and PENK.In some embodiments, the target gene comprises HOXA9, NKX1-1, BNC1, and BCL11B. In some embodiments, the target gene comprises HOXA9, NKX1-1, BNC1, and ALX1. In some embodiments, the target gene comprises HOXA9, NKX1-1, IRX4, and SLC26A4. In some embodiments, the target gene comprises HOXA9, NKX1-1, IRX4, and TBX15. In some embodiments, the target gene comprises HOXA9, NKX1-1, IRX4, and NKX2-8. In some embodiments, the target gene comprises HOXA9, NKX1-1, IRX4, and NRN1. In some embodiments, the target gene comprises HOXA9, NKX1-1, IRX4, and ZNF154. In some embodiments, the target gene comprises HOXA9, NKX1-1, IRX4, and TRPS1. In some embodiments, the target gene comprises HOXA9, NKX1-1, IRX4, and RAB37. In some embodiments, the target gene comprises HOXA9, NKX1-1, IRX4, and SEPT9. In some embodiments, the target gene comprises HOXA9, NKX1-1, IRX4, and LRRC9. In some embodiments, the target gene comprises HOXA9, NKX1-1, IRX4, and PAX1. In some embodiments, the target gene comprises HOXA9, NKX1-1, IRX4, and KCNMA1. In some embodiments, the target gene comprises HOXA9, NKX1-1, IRX4, and FOXF2. In some embodiments, the target gene comprises HOXA9, NKX1-1, IRX4, and LHX8. In some embodiments, the target gene comprises HOXA9, NKX1-1, IRX4, and NKX6-2. In some embodiments, the target gene comprises HOXA9, NKX1-1, IRX4, and PENK. In some embodiments, the target gene comprises HOXA9, NKX1-1, IRX4, and BCL11B. In some embodiments, the target gene comprises HOXA9, NKX1-1, IRX4, and ALX1. In some embodiments, the target gene comprises HOXA9, NKX1-1, SLC26A4, and TBX15. In some embodiments, the target gene comprises HOXA9, NKX1-1, SLC26A4, and NKX2-8. In some embodiments, the target gene comprises HOXA9, NKX1-1, SLC26A4, and NRN1. In some embodiments, the target gene comprises HOXA9, NKX1-1, SLC26A4, and ZNF154.In some embodiments, the target gene comprises HOXA9, NKX1-1, SLC26A4, and TRPS1. In some embodiments, the target gene comprises HOXA9, NKX1-1, SLC26A4, and RAB37. In some embodiments, the target gene comprises HOXA9, NKX1-1, SLC26A4, and SEPT9. In some embodiments, the target gene comprises HOXA9, NKX1-1, SLC26A4, and LRRC9. In some embodiments, the target gene comprises HOXA9, NKX1-1, SLC26A4, and PAX1. In some embodiments, the target gene comprises HOXA9, NKX1-1, SLC26A4, and KCNMA1. In some embodiments, the target gene comprises HOXA9, NKX1-1, SLC26A4, and FOXF2. In some embodiments, the target gene comprises HOXA9, NKX1-1, SLC26A4, and LHX8. In some embodiments, the target gene comprises HOXA9, NKX1-1, SLC26A4, and NKX6-2. In some embodiments, the target gene comprises HOXA9, NKX1-1, SLC26A4, and PENK. In some embodiments, the target gene comprises HOXA9, NKX1-1, SLC26A4, and BCL11B. In some embodiments, the target gene comprises HOXA9, NKX1-1, SLC26A4, and ALX1. In some embodiments, the target gene comprises HOXA9, NKX1-1, TBX15, and NKX2-8. In some embodiments, the target gene comprises HOXA9, NKX1-1, TBX15, and NRN1. In some embodiments, the target gene comprises HOXA9, NKX1-1, TBX15, and ZNF154. In some embodiments, the target gene comprises HOXA9, NKX1-1, TBX15, and TRPS1. In some embodiments, the target gene comprises HOXA9, NKX1-1, TBX15, and RAB37. In some embodiments, the target gene comprises HOXA9, NKX1-1, TBX15, and SEPT9. In some embodiments, the target gene comprises HOXA9, NKX1-1, TBX15, and LRRC9. In some embodiments, the target gene comprises HOXA9, NKX1-1, TBX15, and PAX1. In some embodiments, the target gene comprises HOXA9, NKX1-1, TBX15, and KCNMA1. In some embodiments, the target gene comprises HOXA9, NKX1-1, TBX15, and FOXF2. In some embodiments, the target gene comprises HOXA9, NKX1-1, TBX15, and LHX8.In some embodiments, the target gene comprises HOXA9, NKX1-1, TBX15, and NKX6-2. In some embodiments, the target gene comprises HOXA9, NKX1-1, TBX15, and PENK. In some embodiments, the target gene comprises HOXA9, NKX1-1, TBX15, and BCL11B. In some embodiments, the target gene comprises HOXA9, NKX1-1, TBX15, and ALX1. In some embodiments, the target gene comprises HOXA9, NKX1-1, NKX2-8, and NRN1. In some embodiments, the target gene comprises HOXA9, NKX1-1, NKX2-8, and ZNF154. In some embodiments, the target gene comprises HOXA9, NKX1-1, NKX2-8, and TRPS1. In some embodiments, the target gene comprises HOXA9, NKX1-1, NKX2-8, and RAB37. In some embodiments, the target gene comprises HOXA9, NKX1-1, NKX2-8, and SEPT9. In some embodiments, the target gene comprises HOXA9, NKX1-1, NKX2-8, and LRRC9. In some embodiments, the target gene comprises HOXA9, NKX1-1, NKX2-8, and PAX1. In some embodiments, the target gene comprises HOXA9, NKX1-1, NKX2-8, and KCNMA1. In some embodiments, the target gene comprises HOXA9, NKX1-1, NKX2-8, and FOXF2. In some embodiments, the target gene comprises HOXA9, NKX1-1, NKX2-8, and LHX8. In some embodiments, the target gene comprises HOXA9, NKX1-1, NKX2-8, and NKX6-2. In some embodiments, the target gene comprises HOXA9, NKX1-1, NKX2-8, and PENK. In some embodiments, the target gene comprises HOXA9, NKX1-1, NKX2-8, and BCL11B. In some embodiments, the target gene comprises HOXA9, NKX1-1, NKX2-8, and ALX1. In some embodiments, the target gene comprises HOXA9, NKX1-1, NRN1, and ZNF154. In some embodiments, the target gene comprises HOXA9, NKX1-1, NRN1, and TRPS1. In some embodiments, the target gene comprises HOXA9, NKX1-1, NRN1, and RAB37. In some embodiments, the target gene comprises HOXA9, NKX1-1, NRN1, and SEPT9. In some embodiments, the target gene comprises HOXA9, NKX1-1, NRN1, and LRRC9.In some embodiments, the target gene comprises HOXA9, NKX1-1, NRN1, and PAX1. In some embodiments, the target gene comprises HOXA9, NKX1-1, NRN1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, NKX1-1, NRN1, and FOXF2. In some embodiments, the target gene comprises HOXA9, NKX1-1, NRN1, and LHX8. In some embodiments, the target gene comprises HOXA9, NKX1-1, NRN1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, NKX1-1, NRN1, and PENK. In some embodiments, the target gene comprises HOXA9, NKX1-1, NRN1, and BCL11B. In some embodiments, the target gene comprises HOXA9, NKX1-1, NRN1, and ALX1. In some embodiments, the target gene comprises HOXA9, NKX1-1, ZNF154, and TRPS1. In some embodiments, the target gene comprises HOXA9, NKX1-1, ZNF154, and RAB37. In some embodiments, the target gene comprises HOXA9, NKX1-1, ZNF154, and SEPT9. In some embodiments, the target gene comprises HOXA9, NKX1-1, ZNF154, and LRRC9. In some embodiments, the target gene comprises HOXA9, NKX1-1, ZNF154, and PAX1. In some embodiments, the target gene comprises HOXA9, NKX1-1, ZNF154, and KCNMA1. In some embodiments, the target gene comprises HOXA9, NKX1-1, ZNF154, and FOXF2. In some embodiments, the target gene comprises HOXA9, NKX1-1, ZNF154, and LHX8. In some embodiments, the target gene comprises HOXA9, NKX1-1, ZNF154, and NKX6-2. In some embodiments, the target gene comprises HOXA9, NKX1-1, ZNF154, and PENK. In some embodiments, the target gene comprises HOXA9, NKX1-1, ZNF154, and BCL11B. In some embodiments, the target gene comprises HOXA9, NKX1-1, ZNF154, and ALX1. In some embodiments, the target gene comprises HOXA9, NKX1-1, TRPS1, and RAB37. In some embodiments, the target gene comprises HOXA9, NKX1-1, TRPS1, and SEPT9. In some embodiments, the target gene comprises HOXA9, NKX1-1, TRPS1, and LRRC9.In some embodiments, the target gene comprises HOXA9, NKX1-1, TRPS1, and PAX1. In some embodiments, the target gene comprises HOXA9, NKX1-1, TRPS1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, NKX1-1, TRPS1, and FOXF2. In some embodiments, the target gene comprises HOXA9, NKX1-1, TRPS1, and LHX8. In some embodiments, the target gene comprises HOXA9, NKX1-1, TRPS1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, NKX1-1, TRPS1, and PENK. In some embodiments, the target gene comprises HOXA9, NKX1-1, TRPS1, and BCL11B. In some embodiments, the target gene comprises HOXA9, NKX1-1, TRPS1, and ALX1. In some embodiments, the target gene comprises HOXA9, NKX1-1, RAB37, and SEPT9. In some embodiments, the target gene comprises HOXA9, NKX1-1, RAB37, and LRRC9. In some embodiments, the target gene comprises HOXA9, NKX1-1, RAB37, and PAX1. In some embodiments, the target gene comprises HOXA9, NKX1-1, RAB37, and KCNMA1. In some embodiments, the target gene comprises HOXA9, NKX1-1, RAB37, and FOXF2. In some embodiments, the target gene comprises HOXA9, NKX1-1, RAB37, and LHX8. In some embodiments, the target gene comprises HOXA9, NKX1-1, RAB37, and NKX6-2. In some embodiments, the target gene comprises HOXA9, NKX1-1, RAB37, and PENK. In some embodiments, the target gene comprises HOXA9, NKX1-1, RAB37, and BCL11B. In some embodiments, the target gene comprises HOXA9, NKX1-1, RAB37, and ALX1. In some embodiments, the target gene comprises HOXA9, NKX1-1, SEPT9, and LRRC9. In some embodiments, the target gene comprises HOXA9, NKX1-1, SEPT9, and PAX1. In some embodiments, the target gene comprises HOXA9, NKX1-1, SEPT9, and KCNMA1. In some embodiments, the target gene comprises HOXA9, NKX1-1, SEPT9, and FOXF2. In some embodiments, the target gene comprises HOXA9, NKX1-1, SEPT9, and LHX8.In some embodiments, the target gene comprises HOXA9, NKX1-1, SEPT9, and NKX6-2. In some embodiments, the target gene comprises HOXA9, NKX1-1, SEPT9, and PENK. In some embodiments, the target gene comprises HOXA9, NKX1-1, SEPT9, and BCL11B. In some embodiments, the target gene comprises HOXA9, NKX1-1, SEPT9, and ALX1. In some embodiments, the target gene comprises HOXA9, NKX1-1, LRRC9, and PAX1. In some embodiments, the target gene comprises HOXA9, NKX1-1, LRRC9, and KCNMA1. In some embodiments, the target gene comprises HOXA9, NKX1-1, LRRC9, and FOXF2. In some embodiments, the target gene comprises HOXA9, NKX1-1, LRRC9, and LHX8. In some embodiments, the target gene comprises HOXA9, NKX1-1, LRRC9, and NKX6-2. In some embodiments, the target gene comprises HOXA9, NKX1-1, LRRC9, and PENK. In some embodiments, the target gene comprises HOXA9, NKX1-1, LRRC9, and BCL11B. In some embodiments, the target gene comprises HOXA9, NKX1-1, LRRC9, and ALX1. In some embodiments, the target gene comprises HOXA9, NKX1-1, PAX1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, NKX1-1, PAX1, and FOXF2. In some embodiments, the target gene comprises HOXA9, NKX1-1, PAX1, and LHX8. In some embodiments, the target gene comprises HOXA9, NKX1-1, PAX1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, NKX1-1, PAX1, and PENK. In some embodiments, the target gene comprises HOXA9, NKX1-1, PAX1, and BCL11B. In some embodiments, the target gene comprises HOXA9, NKX1-1, PAX1, and ALX1. In some embodiments, the target gene comprises HOXA9, NKX1-1, KCNMA1, and FOXF2. In some embodiments, the target gene comprises HOXA9, NKX1-1, KCNMA1, and LHX8. In some embodiments, the target gene comprises HOXA9, NKX1-1, KCNMA1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, NKX1-1, KCNMA1, and PENK.In some embodiments, the target gene comprises HOXA9, NKX1-1, KCNMA1, and BCL11B. In some embodiments, the target gene comprises HOXA9, NKX1-1, KCNMA1, and ALX1. In some embodiments, the target gene comprises HOXA9, NKX1-1, FOXF2, and LHX8. In some embodiments, the target gene comprises HOXA9, NKX1-1, FOXF2, and NKX6-2. In some embodiments, the target gene comprises HOXA9, NKX1-1, FOXF2, and PENK. In some embodiments, the target gene comprises HOXA9, NKX1-1, FOXF2, and BCL11B. In some embodiments, the target gene comprises HOXA9, NKX1-1, FOXF2, and ALX1. In some embodiments, the target gene comprises HOXA9, NKX1-1, LHX8, and NKX6-2. In some embodiments, the target gene comprises HOXA9, NKX1-1, LHX8, and PENK. In some embodiments, the target gene comprises HOXA9, NKX1-1, LHX8, and BCL11B. In some embodiments, the target gene comprises HOXA9, NKX1-1, LHX8, and ALX1. In some embodiments, the target gene comprises HOXA9, NKX1-1, NKX6-2, and PENK. In some embodiments, the target gene comprises HOXA9, NKX1-1, NKX6-2, and BCL11B. In some embodiments, the target gene comprises HOXA9, NKX1-1, NKX6-2, and ALX1. In some embodiments, the target gene comprises HOXA9, NKX1-1, PENK, and BCL11B. In some embodiments, the target gene comprises HOXA9, NKX1-1, PENK, and ALX1. In some embodiments, the target gene comprises HOXA9, NKX1-1, BCL11B, and ALX1. In some embodiments, the target gene comprises HOXA9, MARCH11, ZIC4, and BNC1. In some embodiments, the target gene comprises HOXA9, MARCH11, ZIC4, and IRX4. In some embodiments, the target gene comprises HOXA9, MARCH11, ZIC4, and SLC26A4. In some embodiments, the target gene comprises HOXA9, MARCH11, ZIC4, and TBX15. In some embodiments, the target gene comprises HOXA9, MARCH11, ZIC4, and NKX2-8. In some embodiments, the target gene comprises HOXA9, MARCH11, ZIC4, and NRN1.In some embodiments, the target gene comprises HOXA9, MARCH11, ZIC4, and ZNF154. In some embodiments, the target gene comprises HOXA9, MARCH11, ZIC4, and TRPS1. In some embodiments, the target gene comprises HOXA9, MARCH11, ZIC4, and RAB37. In some embodiments, the target gene comprises HOXA9, MARCH11, ZIC4, and SEPT9. In some embodiments, the target gene comprises HOXA9, MARCH11, ZIC4, and LRRC9. In some embodiments, the target gene comprises HOXA9, MARCH11, ZIC4, and PAX1. In some embodiments, the target gene comprises HOXA9, MARCH11, ZIC4, and KCNMA1. In some embodiments, the target gene comprises HOXA9, MARCH11, ZIC4, and FOXF2. In some embodiments, the target gene comprises HOXA9, MARCH11, ZIC4, and LHX8. In some embodiments, the target gene comprises HOXA9, MARCH11, ZIC4, and NKX6-2. In some embodiments, the target gene comprises HOXA9, MARCH11, ZIC4, and PENK. In some embodiments, the target gene comprises HOXA9, MARCH11, ZIC4, and BCL11B. In some embodiments, the target gene comprises HOXA9, MARCH11, ZIC4, and ALX1. In some embodiments, the target gene comprises HOXA9, MARCH11, BNC1, and IRX4. In some embodiments, the target gene comprises HOXA9, MARCH11, BNC1, and SLC26A4. In some embodiments, the target gene comprises HOXA9, MARCH11, BNC1, and TBX15. In some embodiments, the target gene comprises HOXA9, MARCH11, BNC1, and NKX2-8. In some embodiments, the target gene comprises HOXA9, MARCH11, BNC1, and NRN1. In some embodiments, the target gene comprises HOXA9, MARCH11, BNC1, and ZNF154. In some embodiments, the target gene comprises HOXA9, MARCH11, BNC1, and TRPS1. In some embodiments, the target gene comprises HOXA9, MARCH11, BNC1, and RAB37. In some embodiments, the target gene comprises HOXA9, MARCH11, BNC1, and SEPT9. In some embodiments, the target gene comprises HOXA9, MARCH11, BNC1, and LRRC9.In some embodiments, the target gene comprises HOXA9, MARCH11, BNC1, and PAX1. In some embodiments, the target gene comprises HOXA9, MARCH11, BNC1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, MARCH11, BNC1, and FOXF2. In some embodiments, the target gene comprises HOXA9, MARCH11, BNC1, and LHX8. In some embodiments, the target gene comprises HOXA9, MARCH11, BNC1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, MARCH11, BNC1, and PENK. In some embodiments, the target gene comprises HOXA9, MARCH11, BNC1, and BCL11B. In some embodiments, the target gene comprises HOXA9, MARCH11, BNC1, and ALX1. In some embodiments, the target gene comprises HOXA9, MARCH11, IRX4, and SLC26A4. In some embodiments, the target gene comprises HOXA9, MARCH11, IRX4, and TBX15. In some embodiments, the target gene comprises HOXA9, MARCH11, IRX4, and NKX2-8. In some embodiments, the target gene comprises HOXA9, MARCH11, IRX4, and NRN1. In some embodiments, the target gene comprises HOXA9, MARCH11, IRX4, and ZNF154. In some embodiments, the target gene comprises HOXA9, MARCH11, IRX4, and TRPS1. In some embodiments, the target gene comprises HOXA9, MARCH11, IRX4, and RAB37. In some embodiments, the target gene comprises HOXA9, MARCH11, IRX4, and SEPT9. In some embodiments, the target gene comprises HOXA9, MARCH11, IRX4, and LRRC9. In some embodiments, the target gene comprises HOXA9, MARCH11, IRX4, and PAX1. In some embodiments, the target gene comprises HOXA9, MARCH11, IRX4, and KCNMA1. In some embodiments, the target gene comprises HOXA9, MARCH11, IRX4, and FOXF2. In some embodiments, the target gene comprises HOXA9, MARCH11, IRX4, and LHX8. In some embodiments, the target gene comprises HOXA9, MARCH11, IRX4, and NKX6-2. In some embodiments, the target gene comprises HOXA9, MARCH11, IRX4, and PENK.In some embodiments, the target gene comprises HOXA9, MARCH11, IRX4, and BCL11B. In some embodiments, the target gene comprises HOXA9, MARCH11, IRX4, and ALX1. In some embodiments, the target gene comprises HOXA9, MARCH11, SLC26A4, and TBX15. In some embodiments, the target gene comprises HOXA9, MARCH11, SLC26A4, and NKX2-8. In some embodiments, the target gene comprises HOXA9, MARCH11, SLC26A4, and NRN1. In some embodiments, the target gene comprises HOXA9, MARCH11, SLC26A4, and ZNF154. In some embodiments, the target gene comprises HOXA9, MARCH11, SLC26A4, and TRPS1. In some embodiments, the target gene comprises HOXA9, MARCH11, SLC26A4, and RAB37. In some embodiments, the target gene comprises HOXA9, MARCH11, SLC26A4, and SEPT9. In some embodiments, the target gene comprises HOXA9, MARCH11, SLC26A4, and LRRC9. In some embodiments, the target gene comprises HOXA9, MARCH11, SLC26A4, and PAX1. In some embodiments, the target gene comprises HOXA9, MARCH11, SLC26A4, and KCNMA1. In some embodiments, the target gene comprises HOXA9, MARCH11, SLC26A4, and FOXF2. In some embodiments, the target gene comprises HOXA9, MARCH11, SLC26A4, and LHX8. In some embodiments, the target gene comprises HOXA9, MARCH11, SLC26A4, and NKX6-2. In some embodiments, the target gene comprises HOXA9, MARCH11, SLC26A4, and PENK. In some embodiments, the target gene comprises HOXA9, MARCH11, SLC26A4, and BCL11B. In some embodiments, the target gene comprises HOXA9, MARCH11, SLC26A4, and ALX1. In some embodiments, the target gene comprises HOXA9, MARCH11, TBX15, and NKX2-8. In some embodiments, the target gene comprises HOXA9, MARCH11, TBX15, and NRN1. In some embodiments, the target gene comprises HOXA9, MARCH11, TBX15, and ZNF154. In some embodiments, the target gene comprises HOXA9, MARCH11, TBX15, and TRPS1.In some embodiments, the target gene comprises HOXA9, MARCH11, TBX15, and RAB37. In some embodiments, the target gene comprises HOXA9, MARCH11, TBX15, and SEPT9. In some embodiments, the target gene comprises HOXA9, MARCH11, TBX15, and LRRC9. In some embodiments, the target gene comprises HOXA9, MARCH11, TBX15, and PAX1. In some embodiments, the target gene comprises HOXA9, MARCH11, TBX15, and KCNMA1. In some embodiments, the target gene comprises HOXA9, MARCH11, TBX15, and FOXF2. In some embodiments, the target gene comprises HOXA9, MARCH11, TBX15, and LHX8. In some embodiments, the target gene comprises HOXA9, MARCH11, TBX15, and NKX6-2. In some embodiments, the target gene comprises HOXA9, MARCH11, TBX15, and PENK. In some embodiments, the target gene comprises HOXA9, MARCH11, TBX15, and BCL11B. In some embodiments, the target gene comprises HOXA9, MARCH11, TBX15, and ALX1. In some embodiments, the target gene comprises HOXA9, MARCH11, NKX2-8, and NRN1. In some embodiments, the target gene comprises HOXA9, MARCH11, NKX2-8, and ZNF154. In some embodiments, the target gene comprises HOXA9, MARCH11, NKX2-8, and TRPS1. In some embodiments, the target gene comprises HOXA9, MARCH11, NKX2-8, and RAB37. In some embodiments, the target gene comprises HOXA9, MARCH11, NKX2-8, and SEPT9. In some embodiments, the target gene comprises HOXA9, MARCH11, NKX2-8, and LRRC9. In some embodiments, the target gene comprises HOXA9, MARCH11, NKX2-8, and PAX1. In some embodiments, the target gene comprises HOXA9, MARCH11, NKX2-8, and KCNMA1. In some embodiments, the target gene comprises HOXA9, MARCH11, NKX2-8, and FOXF2. In some embodiments, the target gene comprises HOXA9, MARCH11, NKX2-8, and LHX8. In some embodiments, the target gene comprises HOXA9, MARCH11, NKX2-8, and NKX6-2. In some embodiments, the target gene comprises HOXA9, MARCH11, NKX2-8, and PENK.In some embodiments, the target gene comprises HOXA9, MARCH11, NKX2-8, and BCL11B. In some embodiments, the target gene comprises HOXA9, MARCH11, NKX2-8, and ALX1. In some embodiments, the target gene comprises HOXA9, MARCH11, NRN1, and ZNF154. In some embodiments, the target gene comprises HOXA9, MARCH11, NRN1, and TRPS1. In some embodiments, the target gene comprises HOXA9, MARCH11, NRN1, and RAB37. In some embodiments, the target gene comprises HOXA9, MARCH11, NRN1, and SEPT9. In some embodiments, the target gene comprises HOXA9, MARCH11, NRN1, and LRRC9. In some embodiments, the target gene comprises HOXA9, MARCH11, NRN1, and PAX1. In some embodiments, the target gene comprises HOXA9, MARCH11, NRN1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, MARCH11, NRN1, and FOXF2. In some embodiments, the target gene comprises HOXA9, MARCH11, NRN1, and LHX8. In some embodiments, the target gene comprises HOXA9, MARCH11, NRN1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, MARCH11, NRN1, and PENK. In some embodiments, the target gene comprises HOXA9, MARCH11, NRN1, and BCL11B. In some embodiments, the target gene comprises HOXA9, MARCH11, NRN1, and ALX1. In some embodiments, the target gene comprises HOXA9, MARCH11, ZNF154, and TRPS1. In some embodiments, the target gene comprises HOXA9, MARCH11, ZNF154, and RAB37. In some embodiments, the target gene comprises HOXA9, MARCH11, ZNF154, and SEPT9. In some embodiments, the target gene comprises HOXA9, MARCH11, ZNF154, and LRRC9. In some embodiments, the target gene comprises HOXA9, MARCH11, ZNF154, and PAX1. In some embodiments, the target gene comprises HOXA9, MARCH11, ZNF154, and KCNMA1. In some embodiments, the target gene comprises HOXA9, MARCH11, ZNF154, and FOXF2. In some embodiments, the target gene comprises HOXA9, MARCH11, ZNF154, and LHX8.In some embodiments, the target gene comprises HOXA9, MARCH11, ZNF154, and NKX6-2. In some embodiments, the target gene comprises HOXA9, MARCH11, ZNF154, and PENK. In some embodiments, the target gene comprises HOXA9, MARCH11, ZNF154, and BCL11B. In some embodiments, the target gene comprises HOXA9, MARCH11, ZNF154, and ALX1. In some embodiments, the target gene comprises HOXA9, MARCH11, TRPS1, and RAB37. In some embodiments, the target gene comprises HOXA9, MARCH11, TRPS1, and SEPT9. In some embodiments, the target gene comprises HOXA9, MARCH11, TRPS1, and LRRC9. In some embodiments, the target gene comprises HOXA9, MARCH11, TRPS1, and PAX1. In some embodiments, the target gene comprises HOXA9, MARCH11, TRPS1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, MARCH11, TRPS1, and FOXF2. In some embodiments, the target gene comprises HOXA9, MARCH11, TRPS1, and LHX8. In some embodiments, the target gene comprises HOXA9, MARCH11, TRPS1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, MARCH11, TRPS1, and PENK. In some embodiments, the target gene comprises HOXA9, MARCH11, TRPS1, and BCL11B. In some embodiments, the target gene comprises HOXA9, MARCH11, TRPS1, and ALX1. In some embodiments, the target gene comprises HOXA9, MARCH11, RAB37, and SEPT9. In some embodiments, the target gene comprises HOXA9, MARCH11, RAB37, and LRRC9. In some embodiments, the target gene comprises HOXA9, MARCH11, RAB37, and PAX1. In some embodiments, the target gene comprises HOXA9, MARCH11, RAB37, and KCNMA1. In some embodiments, the target gene comprises HOXA9, MARCH11, RAB37, and FOXF2. In some embodiments, the target gene comprises HOXA9, MARCH11, RAB37, and LHX8. In some embodiments, the target gene comprises HOXA9, MARCH11, RAB37, and NKX6-2. In some embodiments, the target gene comprises HOXA9, MARCH11, RAB37, and PENK.In some embodiments, the target gene comprises HOXA9, MARCH11, RAB37, and BCL11B. In some embodiments, the target gene comprises HOXA9, MARCH11, RAB37, and ALX1. In some embodiments, the target gene comprises HOXA9, MARCH11, SEPT9, and LRRC9. In some embodiments, the target gene comprises HOXA9, MARCH11, SEPT9, and PAX1. In some embodiments, the target gene comprises HOXA9, MARCH11, SEPT9, and KCNMA1. In some embodiments, the target gene comprises HOXA9, MARCH11, SEPT9, and FOXF2. In some embodiments, the target gene comprises HOXA9, MARCH11, SEPT9, and LHX8. In some embodiments, the target gene comprises HOXA9, MARCH11, SEPT9, and NKX6-2. In some embodiments, the target gene comprises HOXA9, MARCH11, SEPT9, and PENK. In some embodiments, the target gene comprises HOXA9, MARCH11, SEPT9, and BCL11B. In some embodiments, the target gene comprises HOXA9, MARCH11, SEPT9, and ALX1. In some embodiments, the target gene comprises HOXA9, MARCH11, LRRC9, and PAX1. In some embodiments, the target gene comprises HOXA9, MARCH11, LRRC9, and KCNMA1. In some embodiments, the target gene comprises HOXA9, MARCH11, LRRC9, and FOXF2. In some embodiments, the target gene comprises HOXA9, MARCH11, LRRC9, and LHX8. In some embodiments, the target gene comprises HOXA9, MARCH11, LRRC9, and NKX6-2. In some embodiments, the target gene comprises HOXA9, MARCH11, LRRC9, and PENK. In some embodiments, the target gene comprises HOXA9, MARCH11, LRRC9, and BCL11B. In some embodiments, the target gene comprises HOXA9, MARCH11, LRRC9, and ALX1. In some embodiments, the target gene comprises HOXA9, MARCH11, PAX1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, MARCH11, PAX1, and FOXF2. In some embodiments, the target gene comprises HOXA9, MARCH11, PAX1, and LHX8. In some embodiments, the target gene comprises HOXA9, MARCH11, PAX1, and NKX6-2.In some embodiments, the target gene comprises HOXA9, MARCH11, PAX1, and PENK. In some embodiments, the target gene comprises HOXA9, MARCH11, PAX1, and BCL11B. In some embodiments, the target gene comprises HOXA9, MARCH11, PAX1, and ALX1. In some embodiments, the target gene comprises HOXA9, MARCH11, KCNMA1, and FOXF2. In some embodiments, the target gene comprises HOXA9, MARCH11, KCNMA1, and LHX8. In some embodiments, the target gene comprises HOXA9, MARCH11, KCNMA1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, MARCH11, KCNMA1, and PENK. In some embodiments, the target gene comprises HOXA9, MARCH11, KCNMA1, and BCL11B. In some embodiments, the target gene comprises HOXA9, MARCH11, KCNMA1, and ALX1. In some embodiments, the target gene comprises HOXA9, MARCH11, FOXF2, and LHX8. In some embodiments, the target gene comprises HOXA9, MARCH11, FOXF2, and NKX6-2. In some embodiments, the target gene comprises HOXA9, MARCH11, FOXF2, and PENK. In some embodiments, the target gene comprises HOXA9, MARCH11, FOXF2, and BCL11B. In some embodiments, the target gene comprises HOXA9, MARCH11, FOXF2, and ALX1. In some embodiments, the target gene comprises HOXA9, MARCH11, LHX8, and NKX6-2. In some embodiments, the target gene comprises HOXA9, MARCH11, LHX8, and PENK. In some embodiments, the target gene comprises HOXA9, MARCH11, LHX8, and BCL11B. In some embodiments, the target gene comprises HOXA9, MARCH11, LHX8, and ALX1. In some embodiments, the target gene comprises HOXA9, MARCH11, NKX6-2, and PENK. In some embodiments, the target gene comprises HOXA9, MARCH11, NKX6-2, and BCL11B. In some embodiments, the target gene comprises HOXA9, MARCH11, NKX6-2, and ALX1. In some embodiments, the target gene comprises HOXA9, MARCH11, PENK, and BCL11B. In some embodiments, the target gene comprises HOXA9, MARCH11, PENK, and ALX1.In some embodiments, the target gene comprises HOXA9, MARCH11, BCL11B, and ALX1. In some embodiments, the target gene comprises HOXA9, ZIC4, BNC1, and IRX4. In some embodiments, the target gene comprises HOXA9, ZIC4, BNC1, and SLC26A4. In some embodiments, the target gene comprises HOXA9, ZIC4, BNC1, and TBX15. In some embodiments, the target gene comprises HOXA9, ZIC4, BNC1, and NKX2-8. In some embodiments, the target gene comprises HOXA9, ZIC4, BNC1, and NRN1. In some embodiments, the target gene comprises HOXA9, ZIC4, BNC1, and ZNF154. In some embodiments, the target gene comprises HOXA9, ZIC4, BNC1, and TRPS1. In some embodiments, the target gene comprises HOXA9, ZIC4, BNC1, and RAB37. In some embodiments, the target gene comprises HOXA9, ZIC4, BNC1, and SEPT9. In some embodiments, the target gene comprises HOXA9, ZIC4, BNC1, and LRRC9. In some embodiments, the target gene comprises HOXA9, ZIC4, BNC1, and PAX1. In some embodiments, the target gene comprises HOXA9, ZIC4, BNC1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, ZIC4, BNC1, and FOXF2. In some embodiments, the target gene comprises HOXA9, ZIC4, BNC1, and LHX8. In some embodiments, the target gene comprises HOXA9, ZIC4, BNC1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, ZIC4, BNC1, and PENK. In some embodiments, the target gene comprises HOXA9, ZIC4, BNC1, and BCL11B. In some embodiments, the target gene comprises HOXA9, ZIC4, BNC1, and ALX1. In some embodiments, the target gene comprises HOXA9, ZIC4, IRX4, and SLC26A4. In some embodiments, the target gene comprises HOXA9, ZIC4, IRX4, and TBX15. In some embodiments, the target gene comprises HOXA9, ZIC4, IRX4, and NKX2-8. In some embodiments, the target gene comprises HOXA9, ZIC4, IRX4, and NRN1. In some embodiments, the target gene comprises HOXA9, ZIC4, IRX4, and ZNF154. In some embodiments, the target gene comprises HOXA9, ZIC4, IRX4, and TRPS1.In some embodiments, the target gene comprises HOXA9, ZIC4, IRX4, and RAB37. In some embodiments, the target gene comprises HOXA9, ZIC4, IRX4, and SEPT9. In some embodiments, the target gene comprises HOXA9, ZIC4, IRX4, and LRRC9. In some embodiments, the target gene comprises HOXA9, ZIC4, IRX4, and PAX1. In some embodiments, the target gene comprises HOXA9, ZIC4, IRX4, and KCNMA1. In some embodiments, the target gene comprises HOXA9, ZIC4, IRX4, and FOXF2. In some embodiments, the target gene comprises HOXA9, ZIC4, IRX4, and LHX8. In some embodiments, the target gene comprises HOXA9, ZIC4, IRX4, and NKX6-2. In some embodiments, the target gene comprises HOXA9, ZIC4, IRX4, and PENK. In some embodiments, the target gene comprises HOXA9, ZIC4, IRX4, and BCL11B. In some embodiments, the target gene comprises HOXA9, ZIC4, IRX4, and ALX1. In some embodiments, the target gene comprises HOXA9, ZIC4, SLC26A4, and TBX15. In some embodiments, the target gene comprises HOXA9, ZIC4, SLC26A4, and NKX2-8. In some embodiments, the target gene comprises HOXA9, ZIC4, SLC26A4, and NRN1. In some embodiments, the target gene comprises HOXA9, ZIC4, SLC26A4, and ZNF154. In some embodiments, the target gene comprises HOXA9, ZIC4, SLC26A4, and TRPS1. In some embodiments, the target gene comprises HOXA9, ZIC4, SLC26A4, and RAB37. In some embodiments, the target gene comprises HOXA9, ZIC4, SLC26A4, and SEPT9. In some embodiments, the target gene comprises HOXA9, ZIC4, SLC26A4, and LRRC9. In some embodiments, the target gene comprises HOXA9, ZIC4, SLC26A4, and PAX1. In some embodiments, the target gene comprises HOXA9, ZIC4, SLC26A4, and KCNMA1. In some embodiments, the target gene comprises HOXA9, ZIC4, SLC26A4, and FOXF2. In some embodiments, the target gene comprises HOXA9, ZIC4, SLC26A4, and LHX8. In some embodiments, the target gene comprises HOXA9, ZIC4, SLC26A4, and NKX6-2.In some embodiments, the target gene comprises HOXA9, ZIC4, SLC26A4, and PENK. In some embodiments, the target gene comprises HOXA9, ZIC4, SLC26A4, and BCL11B. In some embodiments, the target gene comprises HOXA9, ZIC4, SLC26A4, and ALX1. In some embodiments, the target gene comprises HOXA9, ZIC4, TBX15, and NKX2-8. In some embodiments, the target gene comprises HOXA9, ZIC4, TBX15, and NRN1. In some embodiments, the target gene comprises HOXA9, ZIC4, TBX15, and ZNF154. In some embodiments, the target gene comprises HOXA9, ZIC4, TBX15, and TRPS1. In some embodiments, the target gene comprises HOXA9, ZIC4, TBX15, and RAB37. In some embodiments, the target gene comprises HOXA9, ZIC4, TBX15, and SEPT9. In some embodiments, the target gene comprises HOXA9, ZIC4, TBX15, and LRRC9. In some embodiments, the target gene comprises HOXA9, ZIC4, TBX15, and PAX1. In some embodiments, the target gene comprises HOXA9, ZIC4, TBX15, and KCNMA1. In some embodiments, the target gene comprises HOXA9, ZIC4, TBX15, and FOXF2. In some embodiments, the target gene comprises HOXA9, ZIC4, TBX15, and LHX8. In some embodiments, the target gene comprises HOXA9, ZIC4, TBX15, and NKX6-2. In some embodiments, the target gene comprises HOXA9, ZIC4, TBX15, and PENK. In some embodiments, the target gene comprises HOXA9, ZIC4, TBX15, and BCL11B. In some embodiments, the target gene comprises HOXA9, ZIC4, TBX15, and ALX1. In some embodiments, the target gene comprises HOXA9, ZIC4, NKX2-8, and NRN1. In some embodiments, the target gene comprises HOXA9, ZIC4, NKX2-8, and ZNF154. In some embodiments, the target gene comprises HOXA9, ZIC4, NKX2-8, and TRPS1. In some embodiments, the target gene comprises HOXA9, ZIC4, NKX2-8, and RAB37. In some embodiments, the target gene comprises HOXA9, ZIC4, NKX2-8, and SEPT9. In some embodiments, the target gene comprises HOXA9, ZIC4, NKX2-8, and LRRC9.In some embodiments, the target gene comprises HOXA9, ZIC4, NKX2-8, and PAX1. In some embodiments, the target gene comprises HOXA9, ZIC4, NKX2-8, and KCNMA1. In some embodiments, the target gene comprises HOXA9, ZIC4, NKX2-8, and FOXF2. In some embodiments, the target gene comprises HOXA9, ZIC4, NKX2-8, and LHX8. In some embodiments, the target gene comprises HOXA9, ZIC4, NKX2-8, and NKX6-2. In some embodiments, the target gene comprises HOXA9, ZIC4, NKX2-8, and PENK. In some embodiments, the target gene comprises HOXA9, ZIC4, NKX2-8, and BCL11B. In some embodiments, the target gene comprises HOXA9, ZIC4, NKX2-8, and ALX1. In some embodiments, the target gene comprises HOXA9, ZIC4, NRN1, and ZNF154. In some embodiments, the target gene comprises HOXA9, ZIC4, NRN1, and TRPS1. In some embodiments, the target gene comprises HOXA9, ZIC4, NRN1, and RAB37. In some embodiments, the target gene comprises HOXA9, ZIC4, NRN1, and SEPT9. In some embodiments, the target gene comprises HOXA9, ZIC4, NRN1, and LRRC9. In some embodiments, the target gene comprises HOXA9, ZIC4, NRN1, and PAX1. In some embodiments, the target gene comprises HOXA9, ZIC4, NRN1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, ZIC4, NRN1, and FOXF2. In some embodiments, the target gene comprises HOXA9, ZIC4, NRN1, and LHX8. In some embodiments, the target gene comprises HOXA9, ZIC4, NRN1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, ZIC4, NRN1, and PENK. In some embodiments, the target gene comprises HOXA9, ZIC4, NRN1, and BCL11B. In some embodiments, the target gene comprises HOXA9, ZIC4, NRN1, and ALX1. In some embodiments, the target gene comprises HOXA9, ZIC4, ZNF154, and TRPS1. In some embodiments, the target gene comprises HOXA9, ZIC4, ZNF154, and RAB37. In some embodiments, the target gene comprises HOXA9, ZIC4, ZNF154, and SEPT9. In some embodiments, the target gene comprises HOXA9, ZIC4, ZNF154, and LRRC9.In some embodiments, the target gene comprises HOXA9, ZIC4, ZNF154, and PAX1. In some embodiments, the target gene comprises HOXA9, ZIC4, ZNF154, and KCNMA1. In some embodiments, the target gene comprises HOXA9, ZIC4, ZNF154, and FOXF2. In some embodiments, the target gene comprises HOXA9, ZIC4, ZNF154, and LHX8. In some embodiments, the target gene comprises HOXA9, ZIC4, ZNF154, and NKX6-2. In some embodiments, the target gene comprises HOXA9, ZIC4, ZNF154, and PENK. In some embodiments, the target gene comprises HOXA9, ZIC4, ZNF154, and BCL11B. In some embodiments, the target gene comprises HOXA9, ZIC4, ZNF154, and ALX1. In some embodiments, the target gene comprises HOXA9, ZIC4, TRPS1, and RAB37. In some embodiments, the target gene comprises HOXA9, ZIC4, TRPS1, and SEPT9. In some embodiments, the target gene comprises HOXA9, ZIC4, TRPS1, and LRRC9. In some embodiments, the target gene comprises HOXA9, ZIC4, TRPS1, and PAX1. In some embodiments, the target gene comprises HOXA9, ZIC4, TRPS1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, ZIC4, TRPS1, and FOXF2. In some embodiments, the target gene comprises HOXA9, ZIC4, TRPS1, and LHX8. In some embodiments, the target gene comprises HOXA9, ZIC4, TRPS1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, ZIC4, TRPS1, and PENK. In some embodiments, the target gene comprises HOXA9, ZIC4, TRPS1, and BCL11B. In some embodiments, the target gene comprises HOXA9, ZIC4, TRPS1, and ALX1. In some embodiments, the target gene comprises HOXA9, ZIC4, RAB37, and SEPT9. In some embodiments, the target gene comprises HOXA9, ZIC4, RAB37, and LRRC9. In some embodiments, the target gene comprises HOXA9, ZIC4, RAB37, and PAX1. In some embodiments, the target gene comprises HOXA9, ZIC4, RAB37, and KCNMA1. In some embodiments, the target gene comprises HOXA9, ZIC4, RAB37, and FOXF2.In some embodiments, the target gene comprises HOXA9, ZIC4, RAB37, and LHX8. In some embodiments, the target gene comprises HOXA9, ZIC4, RAB37, and NKX6-2. In some embodiments, the target gene comprises HOXA9, ZIC4, RAB37, and PENK. In some embodiments, the target gene comprises HOXA9, ZIC4, RAB37, and BCL11B. In some embodiments, the target gene comprises HOXA9, ZIC4, RAB37, and ALX1. In some embodiments, the target gene comprises HOXA9, ZIC4, SEPT9, and LRRC9. In some embodiments, the target gene comprises HOXA9, ZIC4, SEPT9, and PAX1. In some embodiments, the target gene comprises HOXA9, ZIC4, SEPT9, and KCNMA1. In some embodiments, the target gene comprises HOXA9, ZIC4, SEPT9, and FOXF2. In some embodiments, the target gene comprises HOXA9, ZIC4, SEPT9, and LHX8. In some embodiments, the target gene comprises HOXA9, ZIC4, SEPT9, and NKX6-2. In some embodiments, the target gene comprises HOXA9, ZIC4, SEPT9, and PENK. In some embodiments, the target gene comprises HOXA9, ZIC4, SEPT9, and BCL11B. In some embodiments, the target gene comprises HOXA9, ZIC4, SEPT9, and ALX1. In some embodiments, the target gene comprises HOXA9, ZIC4, LRRC9, and PAX1. In some embodiments, the target gene comprises HOXA9, ZIC4, LRRC9, and KCNMA1. In some embodiments, the target gene comprises HOXA9, ZIC4, LRRC9, and FOXF2. In some embodiments, the target gene comprises HOXA9, ZIC4, LRRC9, and LHX8. In some embodiments, the target gene comprises HOXA9, ZIC4, LRRC9, and NKX6-2. In some embodiments, the target gene comprises HOXA9, ZIC4, LRRC9, and PENK. In some embodiments, the target gene comprises HOXA9, ZIC4, LRRC9, and BCL11B. In some embodiments, the target gene comprises HOXA9, ZIC4, LRRC9, and ALX1. In some embodiments, the target gene comprises HOXA9, ZIC4, PAX1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, ZIC4, PAX1, and FOXF2. In some embodiments, the target gene comprises HOXA9, ZIC4, PAX1, and LHX8.In some embodiments, the target gene comprises HOXA9, ZIC4, PAX1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, ZIC4, PAX1, and PENK. In some embodiments, the target gene comprises HOXA9, ZIC4, PAX1, and BCL11B. In some embodiments, the target gene comprises HOXA9, ZIC4, PAX1, and ALX1. In some embodiments, the target gene comprises HOXA9, ZIC4, KCNMA1, and FOXF2. In some embodiments, the target gene comprises HOXA9, ZIC4, KCNMA1, and LHX8. In some embodiments, the target gene comprises HOXA9, ZIC4, KCNMA1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, ZIC4, KCNMA1, and PENK. In some embodiments, the target gene comprises HOXA9, ZIC4, KCNMA1, and BCL11B. In some embodiments, the target gene comprises HOXA9, ZIC4, KCNMA1, and ALX1. In some embodiments, the target gene comprises HOXA9, ZIC4, FOXF2, and LHX8. In some embodiments, the target gene comprises HOXA9, ZIC4, FOXF2, and NKX6-2. In some embodiments, the target gene comprises HOXA9, ZIC4, FOXF2, and PENK. In some embodiments, the target gene comprises HOXA9, ZIC4, FOXF2, and BCL11B. In some embodiments, the target gene comprises HOXA9, ZIC4, FOXF2, and ALX1. In some embodiments, the target gene comprises HOXA9, ZIC4, LHX8, and NKX6-2. In some embodiments, the target gene comprises HOXA9, ZIC4, LHX8, and PENK. In some embodiments, the target gene comprises HOXA9, ZIC4, LHX8, and BCL11B. In some embodiments, the target gene comprises HOXA9, ZIC4, LHX8, and ALX1. In some embodiments, the target gene comprises HOXA9, ZIC4, NKX6-2, and PENK. In some embodiments, the target gene comprises HOXA9, ZIC4, NKX6-2, and BCL11B. In some embodiments, the target gene comprises HOXA9, ZIC4, NKX6-2, and ALX1. In some embodiments, the target gene comprises HOXA9, ZIC4, PENK, and BCL11B. In some embodiments, the target gene comprises HOXA9, ZIC4, PENK, and ALX1. In some embodiments, the target gene comprises HOXA9, ZIC4, BCL11B, and ALX1.In some embodiments, the target gene comprises HOXA9, BNC1, IRX4, and SLC26A4. In some embodiments, the target gene comprises HOXA9, BNC1, IRX4, and TBX15. In some embodiments, the target gene comprises HOXA9, BNC1, IRX4, and NKX2-8. In some embodiments, the target gene comprises HOXA9, BNC1, IRX4, and NRN1. In some embodiments, the target gene comprises HOXA9, BNC1, IRX4, and ZNF154. In some embodiments, the target gene comprises HOXA9, BNC1, IRX4, and TRPS1. In some embodiments, the target gene comprises HOXA9, BNC1, IRX4, and RAB37. In some embodiments, the target gene comprises HOXA9, BNC1, IRX4, and SEPT9. In some embodiments, the target gene comprises HOXA9, BNC1, IRX4, and LRRC9. In some embodiments, the target gene comprises HOXA9, BNC1, IRX4, and PAX1. In some embodiments, the target gene comprises HOXA9, BNC1, IRX4, and KCNMA1. In some embodiments, the target gene comprises HOXA9, BNC1, IRX4, and FOXF2. In some embodiments, the target gene comprises HOXA9, BNC1, IRX4, and LHX8. In some embodiments, the target gene comprises HOXA9, BNC1, IRX4, and NKX6-2. In some embodiments, the target gene comprises HOXA9, BNC1, IRX4, and PENK. In some embodiments, the target gene comprises HOXA9, BNC1, IRX4, and BCL11B. In some embodiments, the target gene comprises HOXA9, BNC1, IRX4, and ALX1. In some embodiments, the target gene comprises HOXA9, BNC1, SLC26A4, and TBX15. In some embodiments, the target gene comprises HOXA9, BNC1, SLC26A4, and NKX2-8. In some embodiments, the target gene comprises HOXA9, BNC1, SLC26A4, and NRN1. In some embodiments, the target gene comprises HOXA9, BNC1, SLC26A4, and ZNF154. In some embodiments, the target gene comprises HOXA9, BNC1, SLC26A4, and TRPS1. In some embodiments, the target gene comprises HOXA9, BNC1, SLC26A4, and RAB37. In some embodiments, the target gene comprises HOXA9, BNC1, SLC26A4, and SEPT9.In some embodiments, the target gene comprises HOXA9, BNC1, SLC26A4, and LRRC9. In some embodiments, the target gene comprises HOXA9, BNC1, SLC26A4, and PAX1. In some embodiments, the target gene comprises HOXA9, BNC1, SLC26A4, and KCNMA1. In some embodiments, the target gene comprises HOXA9, BNC1, SLC26A4, and FOXF2. In some embodiments, the target gene comprises HOXA9, BNC1, SLC26A4, and LHX8. In some embodiments, the target gene comprises HOXA9, BNC1, SLC26A4, and NKX6-2. In some embodiments, the target gene comprises HOXA9, BNC1, SLC26A4, and PENK. In some embodiments, the target gene comprises HOXA9, BNC1, SLC26A4, and BCL11B. In some embodiments, the target gene comprises HOXA9, BNC1, SLC26A4, and ALX1. In some embodiments, the target gene comprises HOXA9, BNC1, TBX15, and NKX2-8. In some embodiments, the target gene comprises HOXA9, BNC1, TBX15, and NRN1. In some embodiments, the target gene comprises HOXA9, BNC1, TBX15, and ZNF154. In some embodiments, the target gene comprises HOXA9, BNC1, TBX15, and TRPS1. In some embodiments, the target gene comprises HOXA9, BNC1, TBX15, and RAB37. In some embodiments, the target gene comprises HOXA9, BNC1, TBX15, and SEPT9. In some embodiments, the target gene comprises HOXA9, BNC1, TBX15, and LRRC9. In some embodiments, the target gene comprises HOXA9, BNC1, TBX15, and PAX1. In some embodiments, the target gene comprises HOXA9, BNC1, TBX15, and KCNMA1. In some embodiments, the target gene comprises HOXA9, BNC1, TBX15, and FOXF2. In some embodiments, the target gene comprises HOXA9, BNC1, TBX15, and LHX8. In some embodiments, the target gene comprises HOXA9, BNC1, TBX15, and NKX6-2. In some embodiments, the target gene comprises HOXA9, BNC1, TBX15, and PENK. In some embodiments, the target gene comprises HOXA9, BNC1, TBX15, and BCL11B. In some embodiments, the target gene comprises HOXA9, BNC1, TBX15, and ALX1.In some embodiments, the target gene comprises HOXA9, BNC1, NKX2-8, and NRN1. In some embodiments, the target gene comprises HOXA9, BNC1, NKX2-8, and ZNF154. In some embodiments, the target gene comprises HOXA9, BNC1, NKX2-8, and TRPS1. In some embodiments, the target gene comprises HOXA9, BNC1, NKX2-8, and RAB37. In some embodiments, the target gene comprises HOXA9, BNC1, NKX2-8, and SEPT9. In some embodiments, the target gene comprises HOXA9, BNC1, NKX2-8, and LRRC9. In some embodiments, the target gene comprises HOXA9, BNC1, NKX2-8, and PAX1. In some embodiments, the target gene comprises HOXA9, BNC1, NKX2-8, and KCNMA1. In some embodiments, the target gene comprises HOXA9, BNC1, NKX2-8, and FOXF2. In some embodiments, the target gene comprises HOXA9, BNC1, NKX2-8, and LHX8. In some embodiments, the target gene comprises HOXA9, BNC1, NKX2-8, and NKX6-2. In some embodiments, the target gene comprises HOXA9, BNC1, NKX2-8, and PENK. In some embodiments, the target gene comprises HOXA9, BNC1, NKX2-8, and BCL11B. In some embodiments, the target gene comprises HOXA9, BNC1, NKX2-8, and ALX1. In some embodiments, the target gene comprises HOXA9, BNC1, NRN1, and ZNF154. In some embodiments, the target gene comprises HOXA9, BNC1, NRN1, and TRPS1. In some embodiments, the target gene comprises HOXA9, BNC1, NRN1, and RAB37. In some embodiments, the target gene comprises HOXA9, BNC1, NRN1, and SEPT9. In some embodiments, the target gene comprises HOXA9, BNC1, NRN1, and LRRC9. In some embodiments, the target gene comprises HOXA9, BNC1, NRN1, and PAX1. In some embodiments, the target gene comprises HOXA9, BNC1, NRN1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, BNC1, NRN1, and FOXF2. In some embodiments, the target gene comprises HOXA9, BNC1, NRN1, and LHX8. In some embodiments, the target gene comprises HOXA9, BNC1, NRN1, and NKX6-2.In some embodiments, the target gene comprises HOXA9, BNC1, NRN1, and PENK. In some embodiments, the target gene comprises HOXA9, BNC1, NRN1, and BCL11B. In some embodiments, the target gene comprises HOXA9, BNC1, NRN1, and ALX1. In some embodiments, the target gene comprises HOXA9, BNC1, ZNF154, and TRPS1. In some embodiments, the target gene comprises HOXA9, BNC1, ZNF154, and RAB37. In some embodiments, the target gene comprises HOXA9, BNC1, ZNF154, and SEPT9. In some embodiments, the target gene comprises HOXA9, BNC1, ZNF154, and LRRC9. In some embodiments, the target gene comprises HOXA9, BNC1, ZNF154, and PAX1. In some embodiments, the target gene comprises HOXA9, BNC1, ZNF154, and KCNMA1. In some embodiments, the target gene comprises HOXA9, BNC1, ZNF154, and FOXF2. In some embodiments, the target gene comprises HOXA9, BNC1, ZNF154, and LHX8. In some embodiments, the target gene comprises HOXA9, BNC1, ZNF154, and NKX6-2. In some embodiments, the target gene comprises HOXA9, BNC1, ZNF154, and PENK. In some embodiments, the target gene comprises HOXA9, BNC1, ZNF154, and BCL11B. In some embodiments, the target gene comprises HOXA9, BNC1, ZNF154, and ALX1. In some embodiments, the target gene comprises HOXA9, BNC1, TRPS1, and RAB37. In some embodiments, the target gene comprises HOXA9, BNC1, TRPS1, and SEPT9. In some embodiments, the target gene comprises HOXA9, BNC1, TRPS1, and LRRC9. In some embodiments, the target gene comprises HOXA9, BNC1, TRPS1, and PAX1. In some embodiments, the target gene comprises HOXA9, BNC1, TRPS1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, BNC1, TRPS1, and FOXF2. In some embodiments, the target gene comprises HOXA9, BNC1, TRPS1, and LHX8. In some embodiments, the target gene comprises HOXA9, BNC1, TRPS1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, BNC1, TRPS1, and PENK.In some embodiments, the target gene comprises HOXA9, BNC1, TRPS1, and BCL11B. In some embodiments, the target gene comprises HOXA9, BNC1, TRPS1, and ALX1. In some embodiments, the target gene comprises HOXA9, BNC1, RAB37, and SEPT9. In some embodiments, the target gene comprises HOXA9, BNC1, RAB37, and LRRC9. In some embodiments, the target gene comprises HOXA9, BNC1, RAB37, and PAX1. In some embodiments, the target gene comprises HOXA9, BNC1, RAB37, and KCNMA1. In some embodiments, the target gene comprises HOXA9, BNC1, RAB37, and FOXF2. In some embodiments, the target gene comprises HOXA9, BNC1, RAB37, and LHX8. In some embodiments, the target gene comprises HOXA9, BNC1, RAB37, and NKX6-2. In some embodiments, the target gene comprises HOXA9, BNC1, RAB37, and PENK. In some embodiments, the target gene comprises HOXA9, BNC1, RAB37, and BCL11B. In some embodiments, the target gene comprises HOXA9, BNC1, RAB37, and ALX1. In some embodiments, the target gene comprises HOXA9, BNC1, SEPT9, and LRRC9. In some embodiments, the target gene comprises HOXA9, BNC1, SEPT9, and PAX1. In some embodiments, the target gene comprises HOXA9, BNC1, SEPT9, and KCNMA1. In some embodiments, the target gene comprises HOXA9, BNC1, SEPT9, and FOXF2. In some embodiments, the target gene comprises HOXA9, BNC1, SEPT9, and LHX8. In some embodiments, the target gene comprises HOXA9, BNC1, SEPT9, and NKX6-2. In some embodiments, the target gene comprises HOXA9, BNC1, SEPT9, and PENK. In some embodiments, the target gene comprises HOXA9, BNC1, SEPT9, and BCL11B. In some embodiments, the target gene comprises HOXA9, BNC1, SEPT9, and ALX1. In some embodiments, the target gene comprises HOXA9, BNC1, LRRC9, and PAX1. In some embodiments, the target gene comprises HOXA9, BNC1, LRRC9, and KCNMA1. In some embodiments, the target gene comprises HOXA9, BNC1, LRRC9, and FOXF2. In some embodiments, the target gene comprises HOXA9, BNC1, LRRC9, and LHX8.In some embodiments, the target gene comprises HOXA9, BNC1, LRRC9, and NKX6-2. In some embodiments, the target gene comprises HOXA9, BNC1, LRRC9, and PENK. In some embodiments, the target gene comprises HOXA9, BNC1, LRRC9, and BCL11B. In some embodiments, the target gene comprises HOXA9, BNC1, LRRC9, and ALX1. In some embodiments, the target gene comprises HOXA9, BNC1, PAX1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, BNC1, PAX1, and FOXF2. In some embodiments, the target gene comprises HOXA9, BNC1, PAX1, and LHX8. In some embodiments, the target gene comprises HOXA9, BNC1, PAX1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, BNC1, PAX1, and PENK. In some embodiments, the target gene comprises HOXA9, BNC1, PAX1, and BCL11B. In some embodiments, the target gene comprises HOXA9, BNC1, PAX1, and ALX1. In some embodiments, the target gene comprises HOXA9, BNC1, KCNMA1, and FOXF2. In some embodiments, the target gene comprises HOXA9, BNC1, KCNMA1, and LHX8. In some embodiments, the target gene comprises HOXA9, BNC1, KCNMA1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, BNC1, KCNMA1, and PENK. In some embodiments, the target gene comprises HOXA9, BNC1, KCNMA1, and BCL11B. In some embodiments, the target gene comprises HOXA9, BNC1, KCNMA1, and ALX1. In some embodiments, the target gene comprises HOXA9, BNC1, FOXF2, and LHX8. In some embodiments, the target gene comprises HOXA9, BNC1, FOXF2, and NKX6-2. In some embodiments, the target gene comprises HOXA9, BNC1, FOXF2, and PENK. In some embodiments, the target gene comprises HOXA9, BNC1, FOXF2, and BCL11B. In some embodiments, the target gene comprises HOXA9, BNC1, FOXF2, and ALX1. In some embodiments, the target gene comprises HOXA9, BNC1, LHX8, and NKX6-2. In some embodiments, the target gene comprises HOXA9, BNC1, LHX8, and PENK. In some embodiments, the target gene comprises HOXA9, BNC1, LHX8, and BCL11B.In some embodiments, the target gene comprises HOXA9, BNC1, LHX8, and ALX1. In some embodiments, the target gene comprises HOXA9, BNC1, NKX6-2, and PENK. In some embodiments, the target gene comprises HOXA9, BNC1, NKX6-2, and BCL11B. In some embodiments, the target gene comprises HOXA9, BNC1, NKX6-2, and ALX1. In some embodiments, the target gene comprises HOXA9, BNC1, PENK, and BCL11B. In some embodiments, the target gene comprises HOXA9, BNC1, PENK, and ALX1. In some embodiments, the target gene comprises HOXA9, BNC1, BCL11B, and ALX1. In some embodiments, the target gene comprises HOXA9, IRX4, SLC26A4, and TBX15. In some embodiments, the target gene comprises HOXA9, IRX4, SLC26A4, and NKX2-8. In some embodiments, the target gene comprises HOXA9, IRX4, SLC26A4, and NRN1. In some embodiments, the target gene comprises HOXA9, IRX4, SLC26A4, and ZNF154. In some embodiments, the target gene comprises HOXA9, IRX4, SLC26A4, and TRPS1. In some embodiments, the target gene comprises HOXA9, IRX4, SLC26A4, and RAB37. In some embodiments, the target gene comprises HOXA9, IRX4, SLC26A4, and SEPT9. In some embodiments, the target gene comprises HOXA9, IRX4, SLC26A4, and LRRC9. In some embodiments, the target gene comprises HOXA9, IRX4, SLC26A4, and PAX1. In some embodiments, the target gene comprises HOXA9, IRX4, SLC26A4, and KCNMA1. In some embodiments, the target gene comprises HOXA9, IRX4, SLC26A4, and FOXF2. In some embodiments, the target gene comprises HOXA9, IRX4, SLC26A4, and LHX8. In some embodiments, the target gene comprises HOXA9, IRX4, SLC26A4, and NKX6-2. In some embodiments, the target gene comprises HOXA9, IRX4, SLC26A4, and PENK. In some embodiments, the target gene comprises HOXA9, IRX4, SLC26A4, and BCL11B. In some embodiments, the target gene comprises HOXA9, IRX4, SLC26A4, and ALX1. In some embodiments, the target gene comprises HOXA9, IRX4, TBX15, and NKX2-8.In some embodiments, the target gene comprises HOXA9, IRX4, TBX15, and NRN1. In some embodiments, the target gene comprises HOXA9, IRX4, TBX15, and ZNF154. In some embodiments, the target gene comprises HOXA9, IRX4, TBX15, and TRPS1. In some embodiments, the target gene comprises HOXA9, IRX4, TBX15, and RAB37. In some embodiments, the target gene comprises HOXA9, IRX4, TBX15, and SEPT9. In some embodiments, the target gene comprises HOXA9, IRX4, TBX15, and LRRC9. In some embodiments, the target gene comprises HOXA9, IRX4, TBX15, and PAX1. In some embodiments, the target gene comprises HOXA9, IRX4, TBX15, and KCNMA1. In some embodiments, the target gene comprises HOXA9, IRX4, TBX15, and FOXF2. In some embodiments, the target gene comprises HOXA9, IRX4, TBX15, and LHX8. In some embodiments, the target gene comprises HOXA9, IRX4, TBX15, and NKX6-2. In some embodiments, the target gene comprises HOXA9, IRX4, TBX15, and PENK. In some embodiments, the target gene comprises HOXA9, IRX4, TBX15, and BCL11B. In some embodiments, the target gene comprises HOXA9, IRX4, TBX15, and ALX1. In some embodiments, the target gene comprises HOXA9, IRX4, NKX2-8, and NRN1. In some embodiments, the target gene comprises HOXA9, IRX4, NKX2-8, and ZNF154. In some embodiments, the target gene comprises HOXA9, IRX4, NKX2-8, and TRPS1. In some embodiments, the target gene comprises HOXA9, IRX4, NKX2-8, and RAB37. In some embodiments, the target gene comprises HOXA9, IRX4, NKX2-8, and SEPT9. In some embodiments, the target gene comprises HOXA9, IRX4, NKX2-8, and LRRC9. In some embodiments, the target gene comprises HOXA9, IRX4, NKX2-8, and PAX1. In some embodiments, the target gene comprises HOXA9, IRX4, NKX2-8, and KCNMA1. In some embodiments, the target gene comprises HOXA9, IRX4, NKX2-8, and FOXF2. In some embodiments, the target gene comprises HOXA9, IRX4, NKX2-8, and LHX8.In some embodiments, the target gene comprises HOXA9, IRX4, NKX2-8, and NKX6-2. In some embodiments, the target gene comprises HOXA9, IRX4, NKX2-8, and PENK. In some embodiments, the target gene comprises HOXA9, IRX4, NKX2-8, and BCL11B. In some embodiments, the target gene comprises HOXA9, IRX4, NKX2-8, and ALX1. In some embodiments, the target gene comprises HOXA9, IRX4, NRN1, and ZNF154. In some embodiments, the target gene comprises HOXA9, IRX4, NRN1, and TRPS1. In some embodiments, the target gene comprises HOXA9, IRX4, NRN1, and RAB37. In some embodiments, the target gene comprises HOXA9, IRX4, NRN1, and SEPT9. In some embodiments, the target gene comprises HOXA9, IRX4, NRN1, and LRRC9. In some embodiments, the target gene comprises HOXA9, IRX4, NRN1, and PAX1. In some embodiments, the target gene comprises HOXA9, IRX4, NRN1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, IRX4, NRN1, and FOXF2. In some embodiments, the target gene comprises HOXA9, IRX4, NRN1, and LHX8. In some embodiments, the target gene comprises HOXA9, IRX4, NRN1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, IRX4, NRN1, and PENK. In some embodiments, the target gene comprises HOXA9, IRX4, NRN1, and BCL11B. In some embodiments, the target gene comprises HOXA9, IRX4, NRN1, and ALX1. In some embodiments, the target gene comprises HOXA9, IRX4, ZNF154, and TRPS1. In some embodiments, the target gene comprises HOXA9, IRX4, ZNF154, and RAB37. In some embodiments, the target gene comprises HOXA9, IRX4, ZNF154, and SEPT9. In some embodiments, the target gene comprises HOXA9, IRX4, ZNF154, and LRRC9. In some embodiments, the target gene comprises HOXA9, IRX4, ZNF154, and PAX1. In some embodiments, the target gene comprises HOXA9, IRX4, ZNF154, and KCNMA1. In some embodiments, the target gene comprises HOXA9, IRX4, ZNF154, and FOXF2. In some embodiments, the target gene comprises HOXA9, IRX4, ZNF154, and LHX8.In some embodiments, the target gene comprises HOXA9, IRX4, ZNF154, and NKX6-2. In some embodiments, the target gene comprises HOXA9, IRX4, ZNF154, and PENK. In some embodiments, the target gene comprises HOXA9, IRX4, ZNF154, and BCL11B. In some embodiments, the target gene comprises HOXA9, IRX4, ZNF154, and ALX1. In some embodiments, the target gene comprises HOXA9, IRX4, TRPS1, and RAB37. In some embodiments, the target gene comprises HOXA9, IRX4, TRPS1, and SEPT9. In some embodiments, the target gene comprises HOXA9, IRX4, TRPS1, and LRRC9. In some embodiments, the target gene comprises HOXA9, IRX4, TRPS1, and PAX1. In some embodiments, the target gene comprises HOXA9, IRX4, TRPS1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, IRX4, TRPS1, and FOXF2. In some embodiments, the target gene comprises HOXA9, IRX4, TRPS1, and LHX8. In some embodiments, the target gene comprises HOXA9, IRX4, TRPS1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, IRX4, TRPS1, and PENK. In some embodiments, the target gene comprises HOXA9, IRX4, TRPS1, and BCL11B. In some embodiments, the target gene comprises HOXA9, IRX4, TRPS1, and ALX1. In some embodiments, the target gene comprises HOXA9, IRX4, RAB37, and SEPT9. In some embodiments, the target gene comprises HOXA9, IRX4, RAB37, and LRRC9. In some embodiments, the target gene comprises HOXA9, IRX4, RAB37, and PAX1. In some embodiments, the target gene comprises HOXA9, IRX4, RAB37, and KCNMA1. In some embodiments, the target gene comprises HOXA9, IRX4, RAB37, and FOXF2. In some embodiments, the target gene comprises HOXA9, IRX4, RAB37, and LHX8. In some embodiments, the target gene comprises HOXA9, IRX4, RAB37, and NKX6-2. In some embodiments, the target gene comprises HOXA9, IRX4, RAB37, and PENK. In some embodiments, the target gene comprises HOXA9, IRX4, RAB37, and BCL11B.In some embodiments, the target gene comprises HOXA9, IRX4, RAB37, and ALX1. In some embodiments, the target gene comprises HOXA9, IRX4, SEPT9, and LRRC9. In some embodiments, the target gene comprises HOXA9, IRX4, SEPT9, and PAX1. In some embodiments, the target gene comprises HOXA9, IRX4, SEPT9, and KCNMA1. In some embodiments, the target gene comprises HOXA9, IRX4, SEPT9, and FOXF2. In some embodiments, the target gene comprises HOXA9, IRX4, SEPT9, and LHX8. In some embodiments, the target gene comprises HOXA9, IRX4, SEPT9, and NKX6-2. In some embodiments, the target gene comprises HOXA9, IRX4, SEPT9, and PENK. In some embodiments, the target gene comprises HOXA9, IRX4, SEPT9, and BCL11B. In some embodiments, the target gene comprises HOXA9, IRX4, SEPT9, and ALX1. In some embodiments, the target gene comprises HOXA9, IRX4, LRRC9, and PAX1. In some embodiments, the target gene comprises HOXA9, IRX4, LRRC9, and KCNMA1. In some embodiments, the target gene comprises HOXA9, IRX4, LRRC9, and FOXF2. In some embodiments, the target gene comprises HOXA9, IRX4, LRRC9, and LHX8. In some embodiments, the target gene comprises HOXA9, IRX4, LRRC9, and NKX6-2. In some embodiments, the target gene comprises HOXA9, IRX4, LRRC9, and PENK. In some embodiments, the target gene comprises HOXA9, IRX4, LRRC9, and BCL11B. In some embodiments, the target gene comprises HOXA9, IRX4, LRRC9, and ALX1. In some embodiments, the target gene comprises HOXA9, IRX4, PAX1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, IRX4, PAX1, and FOXF2. In some embodiments, the target gene comprises HOXA9, IRX4, PAX1, and LHX8. In some embodiments, the target gene comprises HOXA9, IRX4, PAX1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, IRX4, PAX1, and PENK. In some embodiments, the target gene comprises HOXA9, IRX4, PAX1, and BCL11B. In some embodiments, the target gene comprises HOXA9, IRX4, PAX1, and ALX1.In some embodiments, the target gene comprises HOXA9, IRX4, KCNMA1, and FOXF2. In some embodiments, the target gene comprises HOXA9, IRX4, KCNMA1, and LHX8. In some embodiments, the target gene comprises HOXA9, IRX4, KCNMA1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, IRX4, KCNMA1, and PENK. In some embodiments, the target gene comprises HOXA9, IRX4, KCNMA1, and BCL11B. In some embodiments, the target gene comprises HOXA9, IRX4, KCNMA1, and ALX1. In some embodiments, the target gene comprises HOXA9, IRX4, FOXF2, and LHX8. In some embodiments, the target gene comprises HOXA9, IRX4, FOXF2, and NKX6-2. In some embodiments, the target gene comprises HOXA9, IRX4, FOXF2, and PENK. In some embodiments, the target gene comprises HOXA9, IRX4, FOXF2, and BCL11B. In some embodiments, the target gene comprises HOXA9, IRX4, FOXF2, and ALX1. In some embodiments, the target gene comprises HOXA9, IRX4, LHX8, and NKX6-2. In some embodiments, the target gene comprises HOXA9, IRX4, LHX8, and PENK. In some embodiments, the target gene comprises HOXA9, IRX4, LHX8, and BCL11B. In some embodiments, the target gene comprises HOXA9, IRX4, LHX8, and ALX1. In some embodiments, the target gene comprises HOXA9, IRX4, NKX6-2, and PENK. In some embodiments, the target gene comprises HOXA9, IRX4, NKX6-2, and BCL11B. In some embodiments, the target gene comprises HOXA9, IRX4, NKX6-2, and ALX1. In some embodiments, the target gene comprises HOXA9, IRX4, PENK, and BCL11B. In some embodiments, the target gene comprises HOXA9, IRX4, PENK, and ALX1. In some embodiments, the target gene comprises HOXA9, IRX4, BCL11B, and ALX1. In some embodiments, the target gene comprises HOXA9, SLC26A4, TBX15, and NKX2-8. In some embodiments, the target gene comprises HOXA9, SLC26A4, TBX15, and NRN1. In some embodiments, the target gene comprises HOXA9, SLC26A4, TBX15, and ZNF154.In some embodiments, the target gene comprises HOXA9, SLC26A4, TBX15, and TRPS1. In some embodiments, the target gene comprises HOXA9, SLC26A4, TBX15, and RAB37. In some embodiments, the target gene comprises HOXA9, SLC26A4, TBX15, and SEPT9. In some embodiments, the target gene comprises HOXA9, SLC26A4, TBX15, and LRRC9. In some embodiments, the target gene comprises HOXA9, SLC26A4, TBX15, and PAX1. In some embodiments, the target gene comprises HOXA9, SLC26A4, TBX15, and KCNMA1. In some embodiments, the target gene comprises HOXA9, SLC26A4, TBX15, and FOXF2. In some embodiments, the target gene comprises HOXA9, SLC26A4, TBX15, and LHX8. In some embodiments, the target gene comprises HOXA9, SLC26A4, TBX15, and NKX6-2. In some embodiments, the target gene comprises HOXA9, SLC26A4, TBX15, and PENK. In some embodiments, the target gene comprises HOXA9, SLC26A4, TBX15, and BCL11B. In some embodiments, the target gene comprises HOXA9, SLC26A4, TBX15, and ALX1. In some embodiments, the target gene comprises HOXA9, SLC26A4, NKX2-8, and NRN1. In some embodiments, the target gene comprises HOXA9, SLC26A4, NKX2-8, and ZNF154. In some embodiments, the target gene comprises HOXA9, SLC26A4, NKX2-8, and TRPS1. In some embodiments, the target gene comprises HOXA9, SLC26A4, NKX2-8, and RAB37. In some embodiments, the target gene comprises HOXA9, SLC26A4, NKX2-8, and SEPT9. In some embodiments, the target gene comprises HOXA9, SLC26A4, NKX2-8, and LRRC9. In some embodiments, the target gene comprises HOXA9, SLC26A4, NKX2-8, and PAX1. In some embodiments, the target gene comprises HOXA9, SLC26A4, NKX2-8, and KCNMA1. In some embodiments, the target gene comprises HOXA9, SLC26A4, NKX2-8, and FOXF2. In some embodiments, the target gene comprises HOXA9, SLC26A4, NKX2-8, and LHX8. In some embodiments, the target gene comprises HOXA9, SLC26A4, NKX2-8, and NKX6-2.In some embodiments, the target gene comprises HOXA9, SLC26A4, NKX2-8, and PENK. In some embodiments, the target gene comprises HOXA9, SLC26A4, NKX2-8, and BCL11B. In some embodiments, the target gene comprises HOXA9, SLC26A4, NKX2-8, and ALX1. In some embodiments, the target gene comprises HOXA9, SLC26A4, NRN1, and ZNF154. In some embodiments, the target gene comprises HOXA9, SLC26A4, NRN1, and TRPS1. In some embodiments, the target gene comprises HOXA9, SLC26A4, NRN1, and RAB37. In some embodiments, the target gene comprises HOXA9, SLC26A4, NRN1, and SEPT9. In some embodiments, the target gene comprises HOXA9, SLC26A4, NRN1, and LRRC9. In some embodiments, the target gene comprises HOXA9, SLC26A4, NRN1, and PAX1. In some embodiments, the target gene comprises HOXA9, SLC26A4, NRN1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, SLC26A4, NRN1, and FOXF2. In some embodiments, the target gene comprises HOXA9, SLC26A4, NRN1, and LHX8. In some embodiments, the target gene comprises HOXA9, SLC26A4, NRN1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, SLC26A4, NRN1, and PENK. In some embodiments, the target gene comprises HOXA9, SLC26A4, NRN1, and BCL11B. In some embodiments, the target gene comprises HOXA9, SLC26A4, NRN1, and ALX1. In some embodiments, the target gene comprises HOXA9, SLC26A4, ZNF154, and TRPS1. In some embodiments, the target gene comprises HOXA9, SLC26A4, ZNF154, and RAB37. In some embodiments, the target gene comprises HOXA9, SLC26A4, ZNF154, and SEPT9. In some embodiments, the target gene comprises HOXA9, SLC26A4, ZNF154, and LRRC9. In some embodiments, the target gene comprises HOXA9, SLC26A4, ZNF154, and PAX1. In some embodiments, the target gene comprises HOXA9, SLC26A4, ZNF154, and KCNMA1. In some embodiments, the target gene comprises HOXA9, SLC26A4, ZNF154, and FOXF2.In some embodiments, the target gene comprises HOXA9, SLC26A4, ZNF154, and LHX8. In some embodiments, the target gene comprises HOXA9, SLC26A4, ZNF154, and NKX6-2. In some embodiments, the target gene comprises HOXA9, SLC26A4, ZNF154, and PENK. In some embodiments, the target gene comprises HOXA9, SLC26A4, ZNF154, and BCL11B. In some embodiments, the target gene comprises HOXA9, SLC26A4, ZNF154, and ALX1. In some embodiments, the target gene comprises HOXA9, SLC26A4, TRPS1, and RAB37. In some embodiments, the target gene comprises HOXA9, SLC26A4, TRPS1, and SEPT9. In some embodiments, the target gene comprises HOXA9, SLC26A4, TRPS1, and LRRC9. In some embodiments, the target gene comprises HOXA9, SLC26A4, TRPS1, and PAX1. In some embodiments, the target gene comprises HOXA9, SLC26A4, TRPS1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, SLC26A4, TRPS1, and FOXF2. In some embodiments, the target gene comprises HOXA9, SLC26A4, TRPS1, and LHX8. In some embodiments, the target gene comprises HOXA9, SLC26A4, TRPS1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, SLC26A4, TRPS1, and PENK. In some embodiments, the target gene comprises HOXA9, SLC26A4, TRPS1, and BCL11B. In some embodiments, the target gene comprises HOXA9, SLC26A4, TRPS1, and ALX1. In some embodiments, the target gene comprises HOXA9, SLC26A4, RAB37, and SEPT9. In some embodiments, the target gene comprises HOXA9, SLC26A4, RAB37, and LRRC9. In some embodiments, the target gene comprises HOXA9, SLC26A4, RAB37, and PAX1. In some embodiments, the target gene comprises HOXA9, SLC26A4, RAB37, and KCNMA1. In some embodiments, the target gene comprises HOXA9, SLC26A4, RAB37, and FOXF2. In some embodiments, the target gene comprises HOXA9, SLC26A4, RAB37, and LHX8. In some embodiments, the target gene comprises HOXA9, SLC26A4, RAB37, and NKX6-2.In some embodiments, the target gene comprises HOXA9, SLC26A4, RAB37, and PENK. In some embodiments, the target gene comprises HOXA9, SLC26A4, RAB37, and BCL11B. In some embodiments, the target gene comprises HOXA9, SLC26A4, RAB37, and ALX1. In some embodiments, the target gene comprises HOXA9, SLC26A4, SEPT9, and LRRC9. In some embodiments, the target gene comprises HOXA9, SLC26A4, SEPT9, and PAX1. In some embodiments, the target gene comprises HOXA9, SLC26A4, SEPT9, and KCNMA1. In some embodiments, the target gene comprises HOXA9, SLC26A4, SEPT9, and FOXF2. In some embodiments, the target gene comprises HOXA9, SLC26A4, SEPT9, and LHX8. In some embodiments, the target gene comprises HOXA9, SLC26A4, SEPT9, and NKX6-2. In some embodiments, the target gene comprises HOXA9, SLC26A4, SEPT9, and PENK. In some embodiments, the target gene comprises HOXA9, SLC26A4, SEPT9, and BCL11B. In some embodiments, the target gene comprises HOXA9, SLC26A4, SEPT9, and ALX1. In some embodiments, the target gene comprises HOXA9, SLC26A4, LRRC9, and PAX1. In some embodiments, the target gene comprises HOXA9, SLC26A4, LRRC9, and KCNMA1. In some embodiments, the target gene comprises HOXA9, SLC26A4, LRRC9, and FOXF2. In some embodiments, the target gene comprises HOXA9, SLC26A4, LRRC9, and LHX8. In some embodiments, the target gene comprises HOXA9, SLC26A4, LRRC9, and NKX6-2. In some embodiments, the target gene comprises HOXA9, SLC26A4, LRRC9, and PENK. In some embodiments, the target gene comprises HOXA9, SLC26A4, LRRC9, and BCL11B. In some embodiments, the target gene comprises HOXA9, SLC26A4, LRRC9, and ALX1. In some embodiments, the target gene comprises HOXA9, SLC26A4, PAX1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, SLC26A4, PAX1, and FOXF2. In some embodiments, the target gene comprises HOXA9, SLC26A4, PAX1, and LHX8.In some embodiments, the target gene comprises HOXA9, SLC26A4, PAX1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, SLC26A4, PAX1, and PENK. In some embodiments, the target gene comprises HOXA9, SLC26A4, PAX1, and BCL11B. In some embodiments, the target gene comprises HOXA9, SLC26A4, PAX1, and ALX1. In some embodiments, the target gene comprises HOXA9, SLC26A4, KCNMA1, and FOXF2. In some embodiments, the target gene comprises HOXA9, SLC26A4, KCNMA1, and LHX8. In some embodiments, the target gene comprises HOXA9, SLC26A4, KCNMA1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, SLC26A4, KCNMA1, and PENK. In some embodiments, the target gene comprises HOXA9, SLC26A4, KCNMA1, and BCL11B. In some embodiments, the target gene comprises HOXA9, SLC26A4, KCNMA1, and ALX1. In some embodiments, the target gene comprises HOXA9, SLC26A4, FOXF2, and LHX8. In some embodiments, the target gene comprises HOXA9, SLC26A4, FOXF2, and NKX6-2. In some embodiments, the target gene comprises HOXA9, SLC26A4, FOXF2, and PENK. In some embodiments, the target gene comprises HOXA9, SLC26A4, FOXF2, and BCL11B. In some embodiments, the target gene comprises HOXA9, SLC26A4, FOXF2, and ALX1. In some embodiments, the target gene comprises HOXA9, SLC26A4, LHX8, and NKX6-2. In some embodiments, the target gene comprises HOXA9, SLC26A4, LHX8, and PENK. In some embodiments, the target gene comprises HOXA9, SLC26A4, LHX8, and BCL11B. In some embodiments, the target gene comprises HOXA9, SLC26A4, LHX8, and ALX1. In some embodiments, the target gene comprises HOXA9, SLC26A4, NKX6-2, and PENK. In some embodiments, the target gene comprises HOXA9, SLC26A4, NKX6-2, and BCL11B. In some embodiments, the target gene comprises HOXA9, SLC26A4, NKX6-2, and ALX1. In some embodiments, the target gene comprises HOXA9, SLC26A4, PENK, and BCL11B.In some embodiments, the target gene comprises HOXA9, SLC26A4, PENK, and ALX1. In some embodiments, the target gene comprises HOXA9, SLC26A4, BCL11B, and ALX1. In some embodiments, the target gene comprises HOXA9, TBX15, NKX2-8, and NRN1. In some embodiments, the target gene comprises HOXA9, TBX15, NKX2-8, and ZNF154. In some embodiments, the target gene comprises HOXA9, TBX15, NKX2-8, and TRPS1. In some embodiments, the target gene comprises HOXA9, TBX15, NKX2-8, and RAB37. In some embodiments, the target gene comprises HOXA9, TBX15, NKX2-8, and SEPT9. In some embodiments, the target gene comprises HOXA9, TBX15, NKX2-8, and LRRC9. In some embodiments, the target gene comprises HOXA9, TBX15, NKX2-8, and PAX1. In some embodiments, the target gene comprises HOXA9, TBX15, NKX2-8, and KCNMA1. In some embodiments, the target gene comprises HOXA9, TBX15, NKX2-8, and FOXF2. In some embodiments, the target gene comprises HOXA9, TBX15, NKX2-8, and LHX8. In some embodiments, the target gene comprises HOXA9, TBX15, NKX2-8, and NKX6-2. In some embodiments, the target gene comprises HOXA9, TBX15, NKX2-8, and PENK. In some embodiments, the target gene comprises HOXA9, TBX15, NKX2-8, and BCL11B. In some embodiments, the target gene comprises HOXA9, TBX15, NKX2-8, and ALX1. In some embodiments, the target gene comprises HOXA9, TBX15, NRN1, and ZNF154. In some embodiments, the target gene comprises HOXA9, TBX15, NRN1, and TRPS1. In some embodiments, the target gene comprises HOXA9, TBX15, NRN1, and RAB37. In some embodiments, the target gene comprises HOXA9, TBX15, NRN1, and SEPT9. In some embodiments, the target gene comprises HOXA9, TBX15, NRN1, and LRRC9. In some embodiments, the target gene comprises HOXA9, TBX15, NRN1, and PAX1. In some embodiments, the target gene comprises HOXA9, TBX15, NRN1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, TBX15, NRN1, and FOXF2.In some embodiments, the target gene comprises HOXA9, TBX15, NRN1, and LHX8. In some embodiments, the target gene comprises HOXA9, TBX15, NRN1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, TBX15, NRN1, and PENK. In some embodiments, the target gene comprises HOXA9, TBX15, NRN1, and BCL11B. In some embodiments, the target gene comprises HOXA9, TBX15, NRN1, and ALX1. In some embodiments, the target gene comprises HOXA9, TBX15, ZNF154, and TRPS1. In some embodiments, the target gene comprises HOXA9, TBX15, ZNF154, and RAB37. In some embodiments, the target gene comprises HOXA9, TBX15, ZNF154, and SEPT9. In some embodiments, the target gene comprises HOXA9, TBX15, ZNF154, and LRRC9. In some embodiments, the target gene comprises HOXA9, TBX15, ZNF154, and PAX1. In some embodiments, the target gene comprises HOXA9, TBX15, ZNF154, and KCNMA1. In some embodiments, the target gene comprises HOXA9, TBX15, ZNF154, and FOXF2. In some embodiments, the target gene comprises HOXA9, TBX15, ZNF154, and LHX8. In some embodiments, the target gene comprises HOXA9, TBX15, ZNF154, and NKX6-2. In some embodiments, the target gene comprises HOXA9, TBX15, ZNF154, and PENK. In some embodiments, the target gene comprises HOXA9, TBX15, ZNF154, and BCL11B. In some embodiments, the target gene comprises HOXA9, TBX15, ZNF154, and ALX1. In some embodiments, the target gene comprises HOXA9, TBX15, TRPS1, and RAB37. In some embodiments, the target gene comprises HOXA9, TBX15, TRPS1, and SEPT9. In some embodiments, the target gene comprises HOXA9, TBX15, TRPS1, and LRRC9. In some embodiments, the target gene comprises HOXA9, TBX15, TRPS1, and PAX1. In some embodiments, the target gene comprises HOXA9, TBX15, TRPS1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, TBX15, TRPS1, and FOXF2. In some embodiments, the target gene comprises HOXA9, TBX15, TRPS1, and LHX8.In some embodiments, the target gene comprises HOXA9, TBX15, TRPS1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, TBX15, TRPS1, and PENK. In some embodiments, the target gene comprises HOXA9, TBX15, TRPS1, and BCL11B. In some embodiments, the target gene comprises HOXA9, TBX15, TRPS1, and ALX1. In some embodiments, the target gene comprises HOXA9, TBX15, RAB37, and SEPT9. In some embodiments, the target gene comprises HOXA9, TBX15, RAB37, and LRRC9. In some embodiments, the target gene comprises HOXA9, TBX15, RAB37, and PAX1. In some embodiments, the target gene comprises HOXA9, TBX15, RAB37, and KCNMA1. In some embodiments, the target gene comprises HOXA9, TBX15, RAB37, and FOXF2. In some embodiments, the target gene comprises HOXA9, TBX15, RAB37, and LHX8. In some embodiments, the target gene comprises HOXA9, TBX15, RAB37, and NKX6-2. In some embodiments, the target gene comprises HOXA9, TBX15, RAB37, and PENK. In some embodiments, the target gene comprises HOXA9, TBX15, RAB37, and BCL11B. In some embodiments, the target gene comprises HOXA9, TBX15, RAB37, and ALX1. In some embodiments, the target gene comprises HOXA9, TBX15, SEPT9, and LRRC9. In some embodiments, the target gene comprises HOXA9, TBX15, SEPT9, and PAX1. In some embodiments, the target gene comprises HOXA9, TBX15, SEPT9, and KCNMA1. In some embodiments, the target gene comprises HOXA9, TBX15, SEPT9, and FOXF2. In some embodiments, the target gene comprises HOXA9, TBX15, SEPT9, and LHX8. In some embodiments, the target gene comprises HOXA9, TBX15, SEPT9, and NKX6-2. In some embodiments, the target gene comprises HOXA9, TBX15, SEPT9, and PENK. In some embodiments, the target gene comprises HOXA9, TBX15, SEPT9, and BCL11B. In some embodiments, the target gene comprises HOXA9, TBX15, SEPT9, and ALX1. In some embodiments, the target gene comprises HOXA9, TBX15, LRRC9, and PAX1.In some embodiments, the target gene comprises HOXA9, TBX15, LRRC9, and KCNMA1. In some embodiments, the target gene comprises HOXA9, TBX15, LRRC9, and FOXF2. In some embodiments, the target gene comprises HOXA9, TBX15, LRRC9, and LHX8. In some embodiments, the target gene comprises HOXA9, TBX15, LRRC9, and NKX6-2. In some embodiments, the target gene comprises HOXA9, TBX15, LRRC9, and PENK. In some embodiments, the target gene comprises HOXA9, TBX15, LRRC9, and BCL11B. In some embodiments, the target gene comprises HOXA9, TBX15, LRRC9, and ALX1. In some embodiments, the target gene comprises HOXA9, TBX15, PAX1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, TBX15, PAX1, and FOXF2. In some embodiments, the target gene comprises HOXA9, TBX15, PAX1, and LHX8. In some embodiments, the target gene comprises HOXA9, TBX15, PAX1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, TBX15, PAX1, and PENK. In some embodiments, the target gene comprises HOXA9, TBX15, PAX1, and BCL11B. In some embodiments, the target gene comprises HOXA9, TBX15, PAX1, and ALX1. In some embodiments, the target gene comprises HOXA9, TBX15, KCNMA1, and FOXF2. In some embodiments, the target gene comprises HOXA9, TBX15, KCNMA1, and LHX8. In some embodiments, the target gene comprises HOXA9, TBX15, KCNMA1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, TBX15, KCNMA1, and PENK. In some embodiments, the target gene comprises HOXA9, TBX15, KCNMA1, and BCL11B. In some embodiments, the target gene comprises HOXA9, TBX15, KCNMA1, and ALX1. In some embodiments, the target gene comprises HOXA9, TBX15, FOXF2, and LHX8. In some embodiments, the target gene comprises HOXA9, TBX15, FOXF2, and NKX6-2. In some embodiments, the target gene comprises HOXA9, TBX15, FOXF2, and PENK. In some embodiments, the target gene comprises HOXA9, TBX15, FOXF2, and BCL11B.In some embodiments, the target gene comprises HOXA9, TBX15, FOXF2, and ALX1. In some embodiments, the target gene comprises HOXA9, TBX15, LHX8, and NKX6-2. In some embodiments, the target gene comprises HOXA9, TBX15, LHX8, and PENK. In some embodiments, the target gene comprises HOXA9, TBX15, LHX8, and BCL11B. In some embodiments, the target gene comprises HOXA9, TBX15, LHX8, and ALX1. In some embodiments, the target gene comprises HOXA9, TBX15, NKX6-2, and PENK. In some embodiments, the target gene comprises HOXA9, TBX15, NKX6-2, and BCL11B. In some embodiments, the target gene comprises HOXA9, TBX15, NKX6-2, and ALX1. In some embodiments, the target gene comprises HOXA9, TBX15, PENK, and BCL11B. In some embodiments, the target gene comprises HOXA9, TBX15, PENK, and ALX1. In some embodiments, the target gene comprises HOXA9, TBX15, BCL11B, and ALX1. In some embodiments, the target gene comprises HOXA9, NKX2-8, NRN1, and ZNF154. In some embodiments, the target gene comprises HOXA9, NKX2-8, NRN1, and TRPS1. In some embodiments, the target gene comprises HOXA9, NKX2-8, NRN1, and RAB37. In some embodiments, the target gene comprises HOXA9, NKX2-8, NRN1, and SEPT9. In some embodiments, the target gene comprises HOXA9, NKX2-8, NRN1, and LRRC9. In some embodiments, the target gene comprises HOXA9, NKX2-8, NRN1, and PAX1. In some embodiments, the target gene comprises HOXA9, NKX2-8, NRN1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, NKX2-8, NRN1, and FOXF2. In some embodiments, the target gene comprises HOXA9, NKX2-8, NRN1, and LHX8. In some embodiments, the target gene comprises HOXA9, NKX2-8, NRN1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, NKX2-8, NRN1, and PENK. In some embodiments, the target gene comprises HOXA9, NKX2-8, NRN1, and BCL11B. In some embodiments, the target gene comprises HOXA9, NKX2-8, NRN1, and ALX1.In some embodiments, the target gene comprises HOXA9, NKX2-8, ZNF154, and TRPS1. In some embodiments, the target gene comprises HOXA9, NKX2-8, ZNF154, and RAB37. In some embodiments, the target gene comprises HOXA9, NKX2-8, ZNF154, and SEPT9. In some embodiments, the target gene comprises HOXA9, NKX2-8, ZNF154, and LRRC9. In some embodiments, the target gene comprises HOXA9, NKX2-8, ZNF154, and PAX1. In some embodiments, the target gene comprises HOXA9, NKX2-8, ZNF154, and KCNMA1. In some embodiments, the target gene comprises HOXA9, NKX2-8, ZNF154, and FOXF2. In some embodiments, the target gene comprises HOXA9, NKX2-8, ZNF154, and LHX8. In some embodiments, the target gene comprises HOXA9, NKX2-8, ZNF154, and NKX6-2. In some embodiments, the target gene comprises HOXA9, NKX2-8, ZNF154, and PENK. In some embodiments, the target gene comprises HOXA9, NKX2-8, ZNF154, and BCL11B. In some embodiments, the target gene comprises HOXA9, NKX2-8, ZNF154, and ALX1. In some embodiments, the target gene comprises HOXA9, NKX2-8, TRPS1, and RAB37. In some embodiments, the target gene comprises HOXA9, NKX2-8, TRPS1, and SEPT9. In some embodiments, the target gene comprises HOXA9, NKX2-8, TRPS1, and LRRC9. In some embodiments, the target gene comprises HOXA9, NKX2-8, TRPS1, and PAX1. In some embodiments, the target gene comprises HOXA9, NKX2-8, TRPS1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, NKX2-8, TRPS1, and FOXF2. In some embodiments, the target gene comprises HOXA9, NKX2-8, TRPS1, and LHX8. In some embodiments, the target gene comprises HOXA9, NKX2-8, TRPS1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, NKX2-8, TRPS1, and PENK. In some embodiments, the target gene comprises HOXA9, NKX2-8, TRPS1, and BCL11B. In some embodiments, the target gene comprises HOXA9, NKX2-8, TRPS1, and ALX1.In some embodiments, the target gene comprises HOXA9, NKX2-8, RAB37, and SEPT9. In some embodiments, the target gene comprises HOXA9, NKX2-8, RAB37, and LRRC9. In some embodiments, the target gene comprises HOXA9, NKX2-8, RAB37, and PAX1. In some embodiments, the target gene comprises HOXA9, NKX2-8, RAB37, and KCNMA1. In some embodiments, the target gene comprises HOXA9, NKX2-8, RAB37, and FOXF2. In some embodiments, the target gene comprises HOXA9, NKX2-8, RAB37, and LHX8. In some embodiments, the target gene comprises HOXA9, NKX2-8, RAB37, and NKX6-2. In some embodiments, the target gene comprises HOXA9, NKX2-8, RAB37, and PENK. In some embodiments, the target gene comprises HOXA9, NKX2-8, RAB37, and BCL11B. In some embodiments, the target gene comprises HOXA9, NKX2-8, RAB37, and ALX1. In some embodiments, the target gene comprises HOXA9, NKX2-8, SEPT9, and LRRC9. In some embodiments, the target gene comprises HOXA9, NKX2-8, SEPT9, and PAX1. In some embodiments, the target gene comprises HOXA9, NKX2-8, SEPT9, and KCNMA1. In some embodiments, the target gene comprises HOXA9, NKX2-8, SEPT9, and FOXF2. In some embodiments, the target gene comprises HOXA9, NKX2-8, SEPT9, and LHX8. In some embodiments, the target gene comprises HOXA9, NKX2-8, SEPT9, and NKX6-2. In some embodiments, the target gene comprises HOXA9, NKX2-8, SEPT9, and PENK. In some embodiments, the target gene comprises HOXA9, NKX2-8, SEPT9, and BCL11B. In some embodiments, the target gene comprises HOXA9, NKX2-8, SEPT9, and ALX1. In some embodiments, the target gene comprises HOXA9, NKX2-8, LRRC9, and PAX1. In some embodiments, the target gene comprises HOXA9, NKX2-8, LRRC9, and KCNMA1. In some embodiments, the target gene comprises HOXA9, NKX2-8, LRRC9, and FOXF2. In some embodiments, the target gene comprises HOXA9, NKX2-8, LRRC9, and LHX8.In some embodiments, the target gene comprises HOXA9, NKX2-8, LRRC9, and NKX6-2. In some embodiments, the target gene comprises HOXA9, NKX2-8, LRRC9, and PENK. In some embodiments, the target gene comprises HOXA9, NKX2-8, LRRC9, and BCL11B. In some embodiments, the target gene comprises HOXA9, NKX2-8, LRRC9, and ALX1. In some embodiments, the target gene comprises HOXA9, NKX2-8, PAX1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, NKX2-8, PAX1, and FOXF2. In some embodiments, the target gene comprises HOXA9, NKX2-8, PAX1, and LHX8. In some embodiments, the target gene comprises HOXA9, NKX2-8, PAX1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, NKX2-8, PAX1, and PENK. In some embodiments, the target gene comprises HOXA9, NKX2-8, PAX1, and BCL11B. In some embodiments, the target gene comprises HOXA9, NKX2-8, PAX1, and ALX1. In some embodiments, the target gene comprises HOXA9, NKX2-8, KCNMA1, and FOXF2. In some embodiments, the target gene comprises HOXA9, NKX2-8, KCNMA1, and LHX8. In some embodiments, the target gene comprises HOXA9, NKX2-8, KCNMA1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, NKX2-8, KCNMA1, and PENK. In some embodiments, the target gene comprises HOXA9, NKX2-8, KCNMA1, and BCL11B. In some embodiments, the target gene comprises HOXA9, NKX2-8, KCNMA1, and ALX1. In some embodiments, the target gene comprises HOXA9, NKX2-8, FOXF2, and LHX8. In some embodiments, the target gene comprises HOXA9, NKX2-8, FOXF2, and NKX6-2. In some embodiments, the target gene comprises HOXA9, NKX2-8, FOXF2, and PENK. In some embodiments, the target gene comprises HOXA9, NKX2-8, FOXF2, and BCL11B. In some embodiments, the target gene comprises HOXA9, NKX2-8, FOXF2, and ALX1. In some embodiments, the target gene comprises HOXA9, NKX2-8, LHX8, and NKX6-2.In some embodiments, the target gene comprises HOXA9, NKX2-8, LHX8, and PENK. In some embodiments, the target gene comprises HOXA9, NKX2-8, LHX8, and BCL11B. In some embodiments, the target gene comprises HOXA9, NKX2-8, LHX8, and ALX1. In some embodiments, the target gene comprises HOXA9, NKX2-8, NKX6-2, and PENK. In some embodiments, the target gene comprises HOXA9, NKX2-8, NKX6-2, and BCL11B. In some embodiments, the target gene comprises HOXA9, NKX2-8, NKX6-2, and ALX1. In some embodiments, the target gene comprises HOXA9, NKX2-8, PENK, and BCL11B. In some embodiments, the target gene comprises HOXA9, NKX2-8, PENK, and ALX1. In some embodiments, the target gene comprises HOXA9, NKX2-8, BCL11B, and ALX1. In some embodiments, the target gene comprises HOXA9, NRN1, ZNF154, and TRPS1. In some embodiments, the target gene comprises HOXA9, NRN1, ZNF154, and RAB37. In some embodiments, the target gene comprises HOXA9, NRN1, ZNF154, and SEPT9. In some embodiments, the target gene comprises HOXA9, NRN1, ZNF154, and LRRC9. In some embodiments, the target gene comprises HOXA9, NRN1, ZNF154, and PAX1. In some embodiments, the target gene comprises HOXA9, NRN1, ZNF154, and KCNMA1. In some embodiments, the target gene comprises HOXA9, NRN1, ZNF154, and FOXF2. In some embodiments, the target gene comprises HOXA9, NRN1, ZNF154, and LHX8. In some embodiments, the target gene comprises HOXA9, NRN1, ZNF154, and NKX6-2. In some embodiments, the target gene comprises HOXA9, NRN1, ZNF154, and PENK. In some embodiments, the target gene comprises HOXA9, NRN1, ZNF154, and BCL11B. In some embodiments, the target gene comprises HOXA9, NRN1, ZNF154, and ALX1. In some embodiments, the target gene comprises HOXA9, NRN1, TRPS1, and RAB37. In some embodiments, the target gene comprises HOXA9, NRN1, TRPS1, and SEPT9. In some embodiments, the target gene comprises HOXA9, NRN1, TRPS1, and LRRC9.In some embodiments, the target gene comprises HOXA9, NRN1, TRPS1, and PAX1. In some embodiments, the target gene comprises HOXA9, NRN1, TRPS1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, NRN1, TRPS1, and FOXF2. In some embodiments, the target gene comprises HOXA9, NRN1, TRPS1, and LHX8. In some embodiments, the target gene comprises HOXA9, NRN1, TRPS1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, NRN1, TRPS1, and PENK. In some embodiments, the target gene comprises HOXA9, NRN1, TRPS1, and BCL11B. In some embodiments, the target gene comprises HOXA9, NRN1, TRPS1, and ALX1. In some embodiments, the target gene comprises HOXA9, NRN1, RAB37, and SEPT9. In some embodiments, the target gene comprises HOXA9, NRN1, RAB37, and LRRC9. In some embodiments, the target gene comprises HOXA9, NRN1, RAB37, and PAX1. In some embodiments, the target gene comprises HOXA9, NRN1, RAB37, and KCNMA1. In some embodiments, the target gene comprises HOXA9, NRN1, RAB37, and FOXF2. In some embodiments, the target gene comprises HOXA9, NRN1, RAB37, and LHX8. In some embodiments, the target gene comprises HOXA9, NRN1, RAB37, and NKX6-2. In some embodiments, the target gene comprises HOXA9, NRN1, RAB37, and PENK. In some embodiments, the target gene comprises HOXA9, NRN1, RAB37, and BCL11B. In some embodiments, the target gene comprises HOXA9, NRN1, RAB37, and ALX1. In some embodiments, the target gene comprises HOXA9, NRN1, SEPT9, and LRRC9. In some embodiments, the target gene comprises HOXA9, NRN1, SEPT9, and PAX1. In some embodiments, the target gene comprises HOXA9, NRN1, SEPT9, and KCNMA1. In some embodiments, the target gene comprises HOXA9, NRN1, SEPT9, and FOXF2. In some embodiments, the target gene comprises HOXA9, NRN1, SEPT9, and LHX8. In some embodiments, the target gene comprises HOXA9, NRN1, SEPT9, and NKX6-2. In some embodiments, the target gene comprises HOXA9, NRN1, SEPT9, and PENK.In some embodiments, the target gene comprises HOXA9, NRN1, SEPT9, and BCL11B. In some embodiments, the target gene comprises HOXA9, NRN1, SEPT9, and ALX1. In some embodiments, the target gene comprises HOXA9, NRN1, LRRC9, and PAX1. In some embodiments, the target gene comprises HOXA9, NRN1, LRRC9, and KCNMA1. In some embodiments, the target gene comprises HOXA9, NRN1, LRRC9, and FOXF2. In some embodiments, the target gene comprises HOXA9, NRN1, LRRC9, and LHX8. In some embodiments, the target gene comprises HOXA9, NRN1, LRRC9, and NKX6-2. In some embodiments, the target gene comprises HOXA9, NRN1, LRRC9, and PENK. In some embodiments, the target gene comprises HOXA9, NRN1, LRRC9, and BCL11B. In some embodiments, the target gene comprises HOXA9, NRN1, LRRC9, and ALX1. In some embodiments, the target gene comprises HOXA9, NRN1, PAX1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, NRN1, PAX1, and FOXF2. In some embodiments, the target gene comprises HOXA9, NRN1, PAX1, and LHX8. In some embodiments, the target gene comprises HOXA9, NRN1, PAX1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, NRN1, PAX1, and PENK. In some embodiments, the target gene comprises HOXA9, NRN1, PAX1, and BCL11B. In some embodiments, the target gene comprises HOXA9, NRN1, PAX1, and ALX1. In some embodiments, the target gene comprises HOXA9, NRN1, KCNMA1, and FOXF2. In some embodiments, the target gene comprises HOXA9, NRN1, KCNMA1, and LHX8. In some embodiments, the target gene comprises HOXA9, NRN1, KCNMA1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, NRN1, KCNMA1, and PENK. In some embodiments, the target gene comprises HOXA9, NRN1, KCNMA1, and BCL11B. In some embodiments, the target gene comprises HOXA9, NRN1, KCNMA1, and ALX1. In some embodiments, the target gene comprises HOXA9, NRN1, FOXF2, and LHX8. In some embodiments, the target gene comprises HOXA9, NRN1, FOXF2, and NKX6-2.In some embodiments, the target gene comprises HOXA9, NRN1, FOXF2, and PENK. In some embodiments, the target gene comprises HOXA9, NRN1, FOXF2, and BCL11B. In some embodiments, the target gene comprises HOXA9, NRN1, FOXF2, and ALX1. In some embodiments, the target gene comprises HOXA9, NRN1, LHX8, and NKX6-2. In some embodiments, the target gene comprises HOXA9, NRN1, LHX8, and PENK. In some embodiments, the target gene comprises HOXA9, NRN1, LHX8, and BCL11B. In some embodiments, the target gene comprises HOXA9, NRN1, LHX8, and ALX1. In some embodiments, the target gene comprises HOXA9, NRN1, NKX6-2, and PENK. In some embodiments, the target gene comprises HOXA9, NRN1, NKX6-2, and BCL11B. In some embodiments, the target gene comprises HOXA9, NRN1, NKX6-2, and ALX1. In some embodiments, the target gene comprises HOXA9, NRN1, PENK, and BCL11B. In some embodiments, the target gene comprises HOXA9, NRN1, PENK, and ALX1. In some embodiments, the target gene comprises HOXA9, NRN1, BCL11B, and ALX1. In some embodiments, the target gene comprises HOXA9, ZNF154, TRPS1, and RAB37. In some embodiments, the target gene comprises HOXA9, ZNF154, TRPS1, and SEPT9. In some embodiments, the target gene comprises HOXA9, ZNF154, TRPS1, and LRRC9. In some embodiments, the target gene comprises HOXA9, ZNF154, TRPS1, and PAX1. In some embodiments, the target gene comprises HOXA9, ZNF154, TRPS1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, ZNF154, TRPS1, and FOXF2. In some embodiments, the target gene comprises HOXA9, ZNF154, TRPS1, and LHX8. In some embodiments, the target gene comprises HOXA9, ZNF154, TRPS1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, ZNF154, TRPS1, and PENK. In some embodiments, the target gene comprises HOXA9, ZNF154, TRPS1, and BCL11B. In some embodiments, the target gene comprises HOXA9, ZNF154, TRPS1, and ALX1.In some embodiments, the target gene comprises HOXA9, ZNF154, RAB37, and SEPT9. In some embodiments, the target gene comprises HOXA9, ZNF154, RAB37, and LRRC9. In some embodiments, the target gene comprises HOXA9, ZNF154, RAB37, and PAX1. In some embodiments, the target gene comprises HOXA9, ZNF154, RAB37, and KCNMA1. In some embodiments, the target gene comprises HOXA9, ZNF154, RAB37, and FOXF2. In some embodiments, the target gene comprises HOXA9, ZNF154, RAB37, and LHX8. In some embodiments, the target gene comprises HOXA9, ZNF154, RAB37, and NKX6-2. In some embodiments, the target gene comprises HOXA9, ZNF154, RAB37, and PENK. In some embodiments, the target gene comprises HOXA9, ZNF154, RAB37, and BCL11B. In some embodiments, the target gene comprises HOXA9, ZNF154, RAB37, and ALX1. In some embodiments, the target gene comprises HOXA9, ZNF154, SEPT9, and LRRC9. In some embodiments, the target gene comprises HOXA9, ZNF154, SEPT9, and PAX1. In some embodiments, the target gene comprises HOXA9, ZNF154, SEPT9, and KCNMA1. In some embodiments, the target gene comprises HOXA9, ZNF154, SEPT9, and FOXF2. In some embodiments, the target gene comprises HOXA9, ZNF154, SEPT9, and LHX8. In some embodiments, the target gene comprises HOXA9, ZNF154, SEPT9, and NKX6-2. In some embodiments, the target gene comprises HOXA9, ZNF154, SEPT9, and PENK. In some embodiments, the target gene comprises HOXA9, ZNF154, SEPT9, and BCL11B. In some embodiments, the target gene comprises HOXA9, ZNF154, SEPT9, and ALX1. In some embodiments, the target gene comprises HOXA9, ZNF154, LRRC9, and PAX1. In some embodiments, the target gene comprises HOXA9, ZNF154, LRRC9, and KCNMA1. In some embodiments, the target gene comprises HOXA9, ZNF154, LRRC9, and FOXF2. In some embodiments, the target gene comprises HOXA9, ZNF154, LRRC9, and LHX8.In some embodiments, the target gene comprises HOXA9, ZNF154, LRRC9, and NKX6-2. In some embodiments, the target gene comprises HOXA9, ZNF154, LRRC9, and PENK. In some embodiments, the target gene comprises HOXA9, ZNF154, LRRC9, and BCL11B. In some embodiments, the target gene comprises HOXA9, ZNF154, LRRC9, and ALX1. In some embodiments, the target gene comprises HOXA9, ZNF154, PAX1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, ZNF154, PAX1, and FOXF2. In some embodiments, the target gene comprises HOXA9, ZNF154, PAX1, and LHX8. In some embodiments, the target gene comprises HOXA9, ZNF154, PAX1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, ZNF154, PAX1, and PENK. In some embodiments, the target gene comprises HOXA9, ZNF154, PAX1, and BCL11B. In some embodiments, the target gene comprises HOXA9, ZNF154, PAX1, and ALX1. In some embodiments, the target gene comprises HOXA9, ZNF154, KCNMA1, and FOXF2. In some embodiments, the target gene comprises HOXA9, ZNF154, KCNMA1, and LHX8. In some embodiments, the target gene comprises HOXA9, ZNF154, KCNMA1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, ZNF154, KCNMA1, and PENK. In some embodiments, the target gene comprises HOXA9, ZNF154, KCNMA1, and BCL11B. In some embodiments, the target gene comprises HOXA9, ZNF154, KCNMA1, and ALX1. In some embodiments, the target gene comprises HOXA9, ZNF154, FOXF2, and LHX8. In some embodiments, the target gene comprises HOXA9, ZNF154, FOXF2, and NKX6-2. In some embodiments, the target gene comprises HOXA9, ZNF154, FOXF2, and PENK. In some embodiments, the target gene comprises HOXA9, ZNF154, FOXF2, and BCL11B. In some embodiments, the target gene comprises HOXA9, ZNF154, FOXF2, and ALX1. In some embodiments, the target gene comprises HOXA9, ZNF154, LHX8, and NKX6-2.In some embodiments, the target gene comprises HOXA9, ZNF154, LHX8, and PENK. In some embodiments, the target gene comprises HOXA9, ZNF154, LHX8, and BCL11B. In some embodiments, the target gene comprises HOXA9, ZNF154, LHX8, and ALX1. In some embodiments, the target gene comprises HOXA9, ZNF154, NKX6-2, and PENK. In some embodiments, the target gene comprises HOXA9, ZNF154, NKX6-2, and BCL11B. In some embodiments, the target gene comprises HOXA9, ZNF154, NKX6-2, and ALX1. In some embodiments, the target gene comprises HOXA9, ZNF154, PENK, and BCL11B. In some embodiments, the target gene comprises HOXA9, ZNF154, PENK, and ALX1. In some embodiments, the target gene comprises HOXA9, ZNF154, BCL11B, and ALX1. In some embodiments, the target gene comprises HOXA9, TRPS1, RAB37, and SEPT9. In some embodiments, the target gene comprises HOXA9, TRPS1, RAB37, and LRRC9. In some embodiments, the target gene comprises HOXA9, TRPS1, RAB37, and PAX1. In some embodiments, the target gene comprises HOXA9, TRPS1, RAB37, and KCNMA1. In some embodiments, the target gene comprises HOXA9, TRPS1, RAB37, and FOXF2. In some embodiments, the target gene comprises HOXA9, TRPS1, RAB37, and LHX8. In some embodiments, the target gene comprises HOXA9, TRPS1, RAB37, and NKX6-2. In some embodiments, the target gene comprises HOXA9, TRPS1, RAB37, and PENK. In some embodiments, the target gene comprises HOXA9, TRPS1, RAB37, and BCL11B. In some embodiments, the target gene comprises HOXA9, TRPS1, RAB37, and ALX1. In some embodiments, the target gene comprises HOXA9, TRPS1, SEPT9, and LRRC9. In some embodiments, the target gene comprises HOXA9, TRPS1, SEPT9, and PAX1. In some embodiments, the target gene comprises HOXA9, TRPS1, SEPT9, and KCNMA1. In some embodiments, the target gene comprises HOXA9, TRPS1, SEPT9, and FOXF2. In some embodiments, the target gene comprises HOXA9, TRPS1, SEPT9, and LHX8.In some embodiments, the target gene comprises HOXA9, TRPS1, SEPT9, and NKX6-2. In some embodiments, the target gene comprises HOXA9, TRPS1, SEPT9, and PENK. In some embodiments, the target gene comprises HOXA9, TRPS1, SEPT9, and BCL11B. In some embodiments, the target gene comprises HOXA9, TRPS1, SEPT9, and ALX1. In some embodiments, the target gene comprises HOXA9, TRPS1, LRRC9, and PAX1. In some embodiments, the target gene comprises HOXA9, TRPS1, LRRC9, and KCNMA1. In some embodiments, the target gene comprises HOXA9, TRPS1, LRRC9, and FOXF2. In some embodiments, the target gene comprises HOXA9, TRPS1, LRRC9, and LHX8. In some embodiments, the target gene comprises HOXA9, TRPS1, LRRC9, and NKX6-2. In some embodiments, the target gene comprises HOXA9, TRPS1, LRRC9, and PENK. In some embodiments, the target gene comprises HOXA9, TRPS1, LRRC9, and BCL11B. In some embodiments, the target gene comprises HOXA9, TRPS1, LRRC9, and ALX1. In some embodiments, the target gene comprises HOXA9, TRPS1, PAX1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, TRPS1, PAX1, and FOXF2. In some embodiments, the target gene comprises HOXA9, TRPS1, PAX1, and LHX8. In some embodiments, the target gene comprises HOXA9, TRPS1, PAX1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, TRPS1, PAX1, and PENK. In some embodiments, the target gene comprises HOXA9, TRPS1, PAX1, and BCL11B. In some embodiments, the target gene comprises HOXA9, TRPS1, PAX1, and ALX1. In some embodiments, the target gene comprises HOXA9, TRPS1, KCNMA1, and FOXF2. In some embodiments, the target gene comprises HOXA9, TRPS1, KCNMA1, and LHX8. In some embodiments, the target gene comprises HOXA9, TRPS1, KCNMA1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, TRPS1, KCNMA1, and PENK. In some embodiments, the target gene comprises HOXA9, TRPS1, KCNMA1, and BCL11B.In some embodiments, the target gene comprises HOXA9, TRPS1, KCNMA1, and ALX1. In some embodiments, the target gene comprises HOXA9, TRPS1, FOXF2, and LHX8. In some embodiments, the target gene comprises HOXA9, TRPS1, FOXF2, and NKX6-2. In some embodiments, the target gene comprises HOXA9, TRPS1, FOXF2, and PENK. In some embodiments, the target gene comprises HOXA9, TRPS1, FOXF2, and BCL11B. In some embodiments, the target gene comprises HOXA9, TRPS1, FOXF2, and ALX1. In some embodiments, the target gene comprises HOXA9, TRPS1, LHX8, and NKX6-2. In some embodiments, the target gene comprises HOXA9, TRPS1, LHX8, and PENK. In some embodiments, the target gene comprises HOXA9, TRPS1, LHX8, and BCL11B. In some embodiments, the target gene comprises HOXA9, TRPS1, LHX8, and ALX1. In some embodiments, the target gene comprises HOXA9, TRPS1, NKX6-2, and PENK. In some embodiments, the target gene comprises HOXA9, TRPS1, NKX6-2, and BCL11B. In some embodiments, the target gene comprises HOXA9, TRPS1, NKX6-2, and ALX1. In some embodiments, the target gene comprises HOXA9, TRPS1, PENK, and BCL11B. In some embodiments, the target gene comprises HOXA9, TRPS1, PENK, and ALX1. In some embodiments, the target gene comprises HOXA9, TRPS1, BCL11B, and ALX1. In some embodiments, the target gene comprises HOXA9, RAB37, SEPT9, and LRRC9. In some embodiments, the target gene comprises HOXA9, RAB37, SEPT9, and PAX1. In some embodiments, the target gene comprises HOXA9, RAB37, SEPT9, and KCNMA1. In some embodiments, the target gene comprises HOXA9, RAB37, SEPT9, and FOXF2. In some embodiments, the target gene comprises HOXA9, RAB37, SEPT9, and LHX8. In some embodiments, the target gene comprises HOXA9, RAB37, SEPT9, and NKX6-2. In some embodiments, the target gene comprises HOXA9, RAB37, SEPT9, and PENK. In some embodiments, the target gene comprises HOXA9, RAB37, SEPT9, and BCL11B.In some embodiments, the target gene comprises HOXA9, RAB37, SEPT9, and ALX1. In some embodiments, the target gene comprises HOXA9, RAB37, LRRC9, and PAX1. In some embodiments, the target gene comprises HOXA9, RAB37, LRRC9, and KCNMA1. In some embodiments, the target gene comprises HOXA9, RAB37, LRRC9, and FOXF2. In some embodiments, the target gene comprises HOXA9, RAB37, LRRC9, and LHX8. In some embodiments, the target gene comprises HOXA9, RAB37, LRRC9, and NKX6-2. In some embodiments, the target gene comprises HOXA9, RAB37, LRRC9, and PENK. In some embodiments, the target gene comprises HOXA9, RAB37, LRRC9, and BCL11B. In some embodiments, the target gene comprises HOXA9, RAB37, LRRC9, and ALX1. In some embodiments, the target gene comprises HOXA9, RAB37, PAX1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, RAB37, PAX1, and FOXF2. In some embodiments, the target gene comprises HOXA9, RAB37, PAX1, and LHX8. In some embodiments, the target gene comprises HOXA9, RAB37, PAX1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, RAB37, PAX1, and PENK. In some embodiments, the target gene comprises HOXA9, RAB37, PAX1, and BCL11B. In some embodiments, the target gene comprises HOXA9, RAB37, PAX1, and ALX1. In some embodiments, the target gene comprises HOXA9, RAB37, KCNMA1, and FOXF2. In some embodiments, the target gene comprises HOXA9, RAB37, KCNMA1, and LHX8. In some embodiments, the target gene comprises HOXA9, RAB37, KCNMA1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, RAB37, KCNMA1, and PENK. In some embodiments, the target gene comprises HOXA9, RAB37, KCNMA1, and BCL11B. In some embodiments, the target gene comprises HOXA9, RAB37, KCNMA1, and ALX1. In some embodiments, the target gene comprises HOXA9, RAB37, FOXF2, and LHX8. In some embodiments, the target gene comprises HOXA9, RAB37, FOXF2, and NKX6-2.In some embodiments, the target gene comprises HOXA9, RAB37, FOXF2, and PENK. In some embodiments, the target gene comprises HOXA9, RAB37, FOXF2, and BCL11B. In some embodiments, the target gene comprises HOXA9, RAB37, FOXF2, and ALX1. In some embodiments, the target gene comprises HOXA9, RAB37, LHX8, and NKX6-2. In some embodiments, the target gene comprises HOXA9, RAB37, LHX8, and PENK. In some embodiments, the target gene comprises HOXA9, RAB37, LHX8, and BCL11B. In some embodiments, the target gene comprises HOXA9, RAB37, LHX8, and ALX1. In some embodiments, the target gene comprises HOXA9, RAB37, NKX6-2, and PENK. In some embodiments, the target gene comprises HOXA9, RAB37, NKX6-2, and BCL11B. In some embodiments, the target gene comprises HOXA9, RAB37, NKX6-2, and ALX1. In some embodiments, the target gene comprises HOXA9, RAB37, PENK, and BCL11B. In some embodiments, the target gene comprises HOXA9, RAB37, PENK, and ALX1. In some embodiments, the target gene comprises HOXA9, RAB37, BCL11B, and ALX1. In some embodiments, the target gene comprises HOXA9, SEPT9, LRRC9, and PAX1. In some embodiments, the target gene comprises HOXA9, SEPT9, LRRC9, and KCNMA1. In some embodiments, the target gene comprises HOXA9, SEPT9, LRRC9, and FOXF2. In some embodiments, the target gene comprises HOXA9, SEPT9, LRRC9, and LHX8. In some embodiments, the target gene comprises HOXA9, SEPT9, LRRC9, and NKX6-2. In some embodiments, the target gene comprises HOXA9, SEPT9, LRRC9, and PENK. In some embodiments, the target gene comprises HOXA9, SEPT9, LRRC9, and BCL11B. In some embodiments, the target gene comprises HOXA9, SEPT9, LRRC9, and ALX1. In some embodiments, the target gene comprises HOXA9, SEPT9, PAX1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, SEPT9, PAX1, and FOXF2. In some embodiments, the target gene comprises HOXA9, SEPT9, PAX1, and LHX8.In some embodiments, the target gene comprises HOXA9, SEPT9, PAX1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, SEPT9, PAX1, and PENK. In some embodiments, the target gene comprises HOXA9, SEPT9, PAX1, and BCL11B. In some embodiments, the target gene comprises HOXA9, SEPT9, PAX1, and ALX1. In some embodiments, the target gene comprises HOXA9, SEPT9, KCNMA1, and FOXF2. In some embodiments, the target gene comprises HOXA9, SEPT9, KCNMA1, and LHX8. In some embodiments, the target gene comprises HOXA9, SEPT9, KCNMA1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, SEPT9, KCNMA1, and PENK. In some embodiments, the target gene comprises HOXA9, SEPT9, KCNMA1, and BCL11B. In some embodiments, the target gene comprises HOXA9, SEPT9, KCNMA1, and ALX1. In some embodiments, the target gene comprises HOXA9, SEPT9, FOXF2, and LHX8. In some embodiments, the target gene comprises HOXA9, SEPT9, FOXF2, and NKX6-2. In some embodiments, the target gene comprises HOXA9, SEPT9, FOXF2, and PENK. In some embodiments, the target gene comprises HOXA9, SEPT9, FOXF2, and BCL11B. In some embodiments, the target gene comprises HOXA9, SEPT9, FOXF2, and ALX1. In some embodiments, the target gene comprises HOXA9, SEPT9, LHX8, and NKX6-2. In some embodiments, the target gene comprises HOXA9, SEPT9, LHX8, and PENK. In some embodiments, the target gene comprises HOXA9, SEPT9, LHX8, and BCL11B. In some embodiments, the target gene comprises HOXA9, SEPT9, LHX8, and ALX1. In some embodiments, the target gene comprises HOXA9, SEPT9, NKX6-2, and PENK. In some embodiments, the target gene comprises HOXA9, SEPT9, NKX6-2, and BCL11B. In some embodiments, the target gene comprises HOXA9, SEPT9, NKX6-2, and ALX1. In some embodiments, the target gene comprises HOXA9, SEPT9, PENK, and BCL11B. In some embodiments, the target gene comprises HOXA9, SEPT9, PENK, and ALX1.In some embodiments, the target gene comprises HOXA9, SEPT9, BCL11B, and ALX1. In some embodiments, the target gene comprises HOXA9, LRRC9, PAX1, and KCNMA1. In some embodiments, the target gene comprises HOXA9, LRRC9, PAX1, and FOXF2. In some embodiments, the target gene comprises HOXA9, LRRC9, PAX1, and LHX8. In some embodiments, the target gene comprises HOXA9, LRRC9, PAX1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, LRRC9, PAX1, and PENK. In some embodiments, the target gene comprises HOXA9, LRRC9, PAX1, and BCL11B. In some embodiments, the target gene comprises HOXA9, LRRC9, PAX1, and ALX1. In some embodiments, the target gene comprises HOXA9, LRRC9, KCNMA1, and FOXF2. In some embodiments, the target gene comprises HOXA9, LRRC9, KCNMA1, and LHX8. In some embodiments, the target gene comprises HOXA9, LRRC9, KCNMA1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, LRRC9, KCNMA1, and PENK. In some embodiments, the target gene comprises HOXA9, LRRC9, KCNMA1, and BCL11B. In some embodiments, the target gene comprises HOXA9, LRRC9, KCNMA1, and ALX1. In some embodiments, the target gene comprises HOXA9, LRRC9, FOXF2, and LHX8. In some embodiments, the target gene comprises HOXA9, LRRC9, FOXF2, and NKX6-2. In some embodiments, the target gene comprises HOXA9, LRRC9, FOXF2, and PENK. In some embodiments, the target gene comprises HOXA9, LRRC9, FOXF2, and BCL11B. In some embodiments, the target gene comprises HOXA9, LRRC9, FOXF2, and ALX1. In some embodiments, the target gene comprises HOXA9, LRRC9, LHX8, and NKX6-2. In some embodiments, the target gene comprises HOXA9, LRRC9, LHX8, and PENK. In some embodiments, the target gene comprises HOXA9, LRRC9, LHX8, and BCL11B. In some embodiments, the target gene comprises HOXA9, LRRC9, LHX8, and ALX1. In some embodiments, the target gene comprises HOXA9, LRRC9, NKX6-2, and PENK.In some embodiments, the target gene comprises HOXA9, LRRC9, NKX6-2, and BCL11B. In some embodiments, the target gene comprises HOXA9, LRRC9, NKX6-2, and ALX1. In some embodiments, the target gene comprises HOXA9, LRRC9, PENK, and BCL11B. In some embodiments, the target gene comprises HOXA9, LRRC9, PENK, and ALX1. In some embodiments, the target gene comprises HOXA9, LRRC9, BCL11B, and ALX1. In some embodiments, the target gene comprises HOXA9, PAX1, KCNMA1, and FOXF2. In some embodiments, the target gene comprises HOXA9, PAX1, KCNMA1, and LHX8. In some embodiments, the target gene comprises HOXA9, PAX1, KCNMA1, and NKX6-2. In some embodiments, the target gene comprises HOXA9, PAX1, KCNMA1, and PENK. In some embodiments, the target gene comprises HOXA9, PAX1, KCNMA1, and BCL11B. In some embodiments, the target gene comprises HOXA9, PAX1, KCNMA1, and ALX1. In some embodiments, the target gene comprises HOXA9, PAX1, FOXF2, and LHX8. In some embodiments, the target gene comprises HOXA9, PAX1, FOXF2, and NKX6-2. In some embodiments, the target gene comprises HOXA9, PAX1, FOXF2, and PENK. In some embodiments, the target gene comprises HOXA9, PAX1, FOXF2, and BCL11B. In some embodiments, the target gene comprises HOXA9, PAX1, FOXF2, and ALX1. In some embodiments, the target gene comprises HOXA9, PAX1, LHX8, and NKX6-2. In some embodiments, the target gene comprises HOXA9, PAX1, LHX8, and PENK. In some embodiments, the target gene comprises HOXA9, PAX1, LHX8, and BCL11B. In some embodiments, the target gene comprises HOXA9, PAX1, LHX8, and ALX1. In some embodiments, the target gene comprises HOXA9, PAX1, NKX6-2, and PENK. In some embodiments, the target gene comprises HOXA9, PAX1, NKX6-2, and BCL11B. In some embodiments, the target gene comprises HOXA9, PAX1, NKX6-2, and ALX1. In some embodiments, the target gene comprises HOXA9, PAX1, PENK, and BCL11B.In some embodiments, the target gene comprises HOXA9, PAX1, PENK, and ALX1. In some embodiments, the target gene comprises HOXA9, PAX1, BCL11B, and ALX1. In some embodiments, the target gene comprises HOXA9, KCNMA1, FOXF2, and LHX8. In some embodiments, the target gene comprises HOXA9, KCNMA1, FOXF2, and NKX6-2. In some embodiments, the target gene comprises HOXA9, KCNMA1, FOXF2, and PENK. In some embodiments, the target gene comprises HOXA9, KCNMA1, FOXF2, and BCL11B. In some embodiments, the target gene comprises HOXA9, KCNMA1, FOXF2, and ALX1. In some embodiments, the target gene comprises HOXA9, KCNMA1, LHX8, and NKX6-2. In some embodiments, the target gene comprises HOXA9, KCNMA1, LHX8, and PENK. In some embodiments, the target gene comprises HOXA9, KCNMA1, LHX8, and BCL11B. In some embodiments, the target gene comprises HOXA9, KCNMA1, LHX8, and ALX1. In some embodiments, the target gene comprises HOXA9, KCNMA1, NKX6-2, and PENK. In some embodiments, the target gene comprises HOXA9, KCNMA1, NKX6-2, and BCL11B. In some embodiments, the target gene comprises HOXA9, KCNMA1, NKX6-2, and ALX1. In some embodiments, the target gene comprises HOXA9, KCNMA1, PENK, and BCL11B. In some embodiments, the target gene comprises HOXA9, KCNMA1, PENK, and ALX1. In some embodiments, the target gene comprises HOXA9, KCNMA1, BCL11B, and ALX1. In some embodiments, the target gene comprises HOXA9, FOXF2, LHX8, and NKX6-2. In some embodiments, the target gene comprises HOXA9, FOXF2, LHX8, and PENK. In some embodiments, the target gene comprises HOXA9, FOXF2, LHX8, and BCL11B. In some embodiments, the target gene comprises HOXA9, FOXF2, LHX8, and ALX1. In some embodiments, the target gene comprises HOXA9, FOXF2, NKX6-2, and PENK. In some embodiments, the target gene comprises HOXA9, FOXF2, NKX6-2, and BCL11B. In some embodiments, the target gene comprises HOXA9, FOXF2, NKX6-2, and ALX1.In some embodiments, the target gene comprises HOXA9, FOXF2, PENK, and BCL11B. In some embodiments, the target gene comprises HOXA9, FOXF2, PENK, and ALX1. In some embodiments, the target gene comprises HOXA9, FOXF2, BCL11B, and ALX1. In some embodiments, the target gene comprises HOXA9, LHX8, NKX6-2, and PENK. In some embodiments, the target gene comprises HOXA9, LHX8, NKX6-2, and BCL11B. In some embodiments, the target gene comprises HOXA9, LHX8, NKX6-2, and ALX1. In some embodiments, the target gene comprises HOXA9, LHX8, PENK, and BCL11B. In some embodiments, the target gene comprises HOXA9, LHX8, PENK, and ALX1. In some embodiments, the target gene comprises HOXA9, LHX8, BCL11B, and ALX1. In some embodiments, the target gene comprises HOXA9, NKX6-2, PENK, and BCL11B. In some embodiments, the target gene comprises HOXA9, NKX6-2, PENK, and ALX1. In some embodiments, the target gene comprises HOXA9, NKX6-2, BCL11B, and ALX1. In some embodiments, the target gene comprises HOXA9, PENK, BCL11B, and ALX1.

[0026] In some embodiments, the target gene further comprises any four genes selected from the group consisting of: VIM, KCNQ1DN, SIM2, ZNF571-AS1, NKX1-1, MARCH11, ZIC4, BNC1, IRX4, SLC26A4, TBX15, NKX2-8, NRN1, ZNF154, TRPS1, RAB37, SEPT9, LRRC9, PAX1, KCNMA1, FOXF2, LHX8, NKX6-2, PENK, BCL11B, and ALX1.

[0027] In some embodiments, the target gene further comprises any five genes selected from the group consisting of: VIM, KCNQ1DN, SIM2, ZNF571-AS1, NKX1-1, MARCH11, ZIC4, BNC1, IRX4, SLC26A4, TBX15, NKX2-8, NRN1, ZNF154, TRPS1, RAB37, SEPT9, LRRC9, PAX1, KCNMA1, FOXF2, LHX8, NKX6-2, PENK, BCL11B, and ALX1.

[0028] In some embodiments, the DNA region of the VIM gene is derived from the region defined in human chr10:17270431-17272617. In some embodiments, the DNA region of the VIM gene includes the region defined in human chr10:17270431-17272617.

[0029] In some embodiments, the DNA region of the VIM gene is derived from a region selected from the group consisting of: the region defined by human chr10:17271339-17271495, the region defined by human chr10:17270568-17270718, the region defined by human chr10:17271389-17271539, and the region defined by human chr10:17271960-17272110.

[0030] In some embodiments, the DNA region of the VIM gene includes the region defined by human chr10:17271339-17271495. In some embodiments, the DNA region of the VIM gene includes the region defined by human chr10:17270568-17270718. In some embodiments, the DNA region of the VIM gene includes the region defined by human chr10:17271389-17271539. In some embodiments, the DNA region of the VIM gene includes the region defined by human chr10:17271960-17272110.

[0031] In some embodiments, the DNA region of the SIM2 gene is derived from a region selected from the group consisting of: the region defined by human chr21:38068194-38073891, the region defined by human chr21:38076763-38077685, the region defined by human chr21:38079942-38081833, and the region defined by human chr21:38119794-38120742.

[0032] In some embodiments, the DNA region of the SIM2 gene includes the region defined by human chr21:38068194-38073891. In some embodiments, the DNA region of the SIM2 gene includes the region defined by human chr21:38076763-38077685. In some embodiments, the DNA region of the SIM2 gene includes the region defined by human chr21:38079942-38081833. In some embodiments, the DNA region of the SIM2 gene includes the region defined by human chr21:38119794-38120742.

[0033] In some embodiments, the DNA region of the SIM2 gene is derived from a region selected from the group consisting of: the region defined by human chr21:38077180-38077341, the region defined by human chr21:38076818-38076967, the region defined by human chr21:38069922-38070042, and the region defined by human chr21:38080876-38080996.

[0034] In some embodiments, the DNA region of the SIM2 gene includes the region defined by human chr21:38077180-38077341. In some embodiments, the DNA region of the SIM2 gene includes the region defined by human chr21:38076818-38076967. In some embodiments, the DNA region of the SIM2 gene includes the region defined by human chr21:38069922-38070042. In some embodiments, the DNA region of the SIM2 gene includes the region defined by human chr21:38080876-38080996.

[0035] In some implementations, the DNA region containing NKX6-2 is derived from the region defined in human chr 10:134597358-134602649.

[0036] In some implementations, the DNA region containing NKX6-2 is derived from the following defined regions: human chr10:134598412-134598978, human chr10:134598322-134598472, human chr10:134598843-134598993 and human chr10:134600284-134600434.

[0037] In some implementations, the DNA region containing NRN1 is derived from the region defined in human chr 6:5999149-6009299.

[0038] In some implementations, the DNA region containing NRN1 is derived from regions selected from the group defined below: human chr 6:5999149-5999787, human chr 6:6002471-6005125, human chr 6:6006689-6007043, human chr 6:6007388-6007797 and human chr 6:6008857-6009299.

[0039] In some implementations, the DNA region containing NRN1 is derived from the following defined regions: human chr6:6004281-6004796, human chr6:6004653-6004803, human chr6:6007407-6007557 and human chr6:6007496-6007646.

[0040] In some embodiments, the DNA region of the BNC1 gene is derived from a region selected from the group consisting of the region defined in human chr15:83951980-83953930. In some embodiments, the DNA region of the BNC1 gene includes the region defined in human chr15:83951980-83953930.

[0041] In some embodiments, the DNA region of the BNC1 gene is derived from a region selected from the group consisting of: the region defined by human chr15:83951985-83952135, the region defined by human chr15:83952080-83952230, the region defined by human chr15:83952179-83952329, and the region defined by human chr15:83952095-83952245.

[0042] In some embodiments, the DNA region of the BNC1 gene includes the region defined by human chr15:83951985-83952135. In some embodiments, the DNA region of the BNC1 gene includes the region defined by human chr15:83952080-83952230. In some embodiments, the DNA region of the BNC1 gene includes the region defined by human chr15:83952179-83952329. In some embodiments, the DNA region of the BNC1 gene includes the region defined by human chr15:83952095-83952245.

[0043] In some implementations, the DNA region containing PENK is derived from the region defined in human chr 8:57358127-57360815.

[0044] In some implementations, the DNA region containing the PENK is derived from a region selected from the following group of defined regions: human chr 8:57358127-57359415, human chr 8:57359899-57360114, and human chr 8:57360585-57360815.

[0045] In some implementations, the DNA region containing the PENK is derived from the following defined regions: human chr8:57359096-57359246, human chr8:57358537-57358687, human chr8:57358648-57358798, and human chr8:57359203-57359353.

[0046] In some implementations, the DNA region containing MARCH11 is derived from the region defined in human chr 5:16179064-16180420.

[0047] In some implementations, the DNA region containing MARCH11 is derived from the following defined regions: human chr5:16179927-16180077, human chr5:16179913-16180063, human chr5:16179942-16180092, and human chr5:16179981-16180131.

[0048] In some implementations, the DNA region containing NKX1-1 is derived from the region defined in human chr 4:1396292-1402435.

[0049] In some implementations, the DNA region containing NKX1-1 is derived from the following defined regions: human chr4:1399435-1399601, human chr4:1396494-1396614, human chr4:1398516-1398636, and human chr4:1401357-1401477.

[0050] In some implementations, the DNA region containing NKX1-1 is derived from regions selected from the following groups: human chr4:1396292-1401730 and human chr4:1402197-1402435.

[0051] In some implementations, the DNA region containing NKX2-8 is derived from the region defined in human chr 14:37049334-37053690.

[0052] In some implementations, the DNA region containing NKX2-8 is derived from regions selected from the following group of defined regions: human chr14:37049334-37051729, human chr 14:37052537-37052838, and human chr 14:37053134-37053690.

[0053] In some implementations, the DNA region containing NKX2-8 is derived from the following defined regions: human chr14:37049860-37049995, human chr14:37050429-37050549, human chr14:37050668-37050788, and human chr14:37051609-37051729.

[0054] In some embodiments, the DNA region of the IRX4 gene is derived from a region selected from the group consisting of: the region defined by human chr5:1874907-1879032, the region defined by human chr5:1879689-1879939, and the region defined by human chr5:1881924-1887743.

[0055] In some embodiments, the DNA region of the IRX4 gene includes the region defined by human chr5:1874907-1879032. In some embodiments, the DNA region of the IRX4 gene includes the region defined by human chr5:1879689-1879939. In some embodiments, the DNA region of the IRX4 gene includes the region defined by human chr5:1881924-1887743.

[0056] In some embodiments, the DNA region of the IRX4 gene is derived from a region selected from the group consisting of: the region defined by human chr5:1875007-1875157, the region defined by human chr5:1879789-1879939, the region defined by human chr5:1882924-1883074, and the region defined by human chr5:1883722-1883872.

[0057] In some embodiments, the DNA region of the IRX4 gene includes the region defined by human chr5:1875007-1875157. In some embodiments, the DNA region of the IRX4 gene includes the region defined by human chr5:1879789-1879939. In some embodiments, the DNA region of the IRX4 gene includes the region defined by human chr5:1882924-1883074. In some embodiments, the DNA region of the IRX4 gene includes the region defined by human chr5:1883722-1883872.

[0058] In some embodiments, the DNA region of the SLC26A4 gene is derived from the region defined in human chr7:107301205-107302416.

[0059] In some embodiments, the DNA region of the SLC26A4 gene includes the region defined in human chr7:107301205-107302416.

[0060] In some embodiments, the DNA region of the SLC26A4 gene is derived from a region selected from the group consisting of: the region defined by human chr7:107301305-107301455, the region defined by human chr7:107302215-107302365, the region defined by human chr7:107301490-107301640, and the region defined by human chr7:107301803-107301953.

[0061] In some embodiments, the DNA region of the SLC26A4 gene includes the region defined by human chr7:107301305-107301455. In some embodiments, the DNA region of the SLC26A4 gene includes the region defined by human chr7:107302215-107302365. In some embodiments, the DNA region of the SLC26A4 gene includes the region defined by human chr7:107301490-107301640. In some embodiments, the DNA region of the SLC26A4 gene includes the region defined by human chr7:107301803-107301953.

[0062] In some embodiments, the DNA region of the PAX1 gene is derived from a region selected from the group consisting of: the region defined by human chr 20:21683744-21684420, the region defined by human chr 20:21686199-21687689, the region defined by human chr 20:21689758-21690048, and the region defined by human chr 20:21694472-21695344.

[0063] In some embodiments, the DNA region of the PAX1 gene includes the region defined by human chr 20:21683744-21684420. In some embodiments, the DNA region of the PAX1 gene includes the region defined by human chr 20:21686199-21687689. In some embodiments, the DNA region of the PAX1 gene includes the region defined by human chr 20:21689758-21690048. In some embodiments, the DNA region of the PAX1 gene includes the region defined by human chr20:21694472-21695344.

[0064] In some embodiments, the DNA region of the PAX1 gene is derived from a region selected from the group consisting of: the region defined by human chr20:21683844-21683994, the region defined by human chr20:21686299-21686449, the region defined by human chr20:21689868-21690018, and the region defined by human chr20:21694579-21694729.

[0065] In some embodiments, the DNA region of the PAX1 gene includes the region defined by human chr20:21683844-21683994. In some embodiments, the DNA region of the PAX1 gene includes the region defined by human chr20:21686299-21686449. In some embodiments, the DNA region of the PAX1 gene includes the region defined by human chr20:21689868-21690018. In some embodiments, the DNA region of the PAX1 gene includes the region defined by human chr20:21694579-21694729.

[0066] In some implementations, the DNA region containing TRPS1 is derived from the region defined in human chr 8:116660432-116681623.

[0067] In some implementations, the DNA region containing TRPS1 is derived from regions selected from the following group of defined regions: human chr8:116660432-116660747, human chr8:116679698-116679936 and human chr8:116680071-116681623.

[0068] In some implementations, the DNA region containing TRPS1 is derived from the following defined regions: human chr8:116680071-116680227, human chr8:116680170-116680295, human chr8:116680576-116680668 and human chr8:116681554-116681893.

[0069] In some implementations, the DNA region containing KCNQ1DN is derived from the region defined in human chr 11:2890389-2891337.

[0070] In some implementations, the DNA region containing KCNQ1DN is derived from regions selected from the following group of defined regions: human chr11:2891024-2891219, human chr11:2890549-2890699, human chr11:2890571-2890721, and human chr11:2891030-2891180.

[0071] In some implementations, the DNA region containing BCL11B is derived from the region defined in human chr 14:99640360-99740920.

[0072] In some implementations, the DNA region containing BCL11B is derived from regions selected from the following group of defined regions: human chr14:99640360-99642767, human chr 14:99711839-99713431, human chr 14:99736040-99737584, and human chr14:99739801-99740920.

[0073] In some implementations, the DNA region containing BCL11B is derived from the following defined regions: human chr14:99712310-99712440, human chr14:99712410-99712560, human chr14:99711939-99712089, and human chr14:99736140-99736290.

[0074] In some implementations, the DNA region containing LHX8 is derived from the region defined in human chr 1:75595813-75602878.

[0075] In some implementations, the DNA region containing LHX8 is derived from regions selected from the following groups: human chr 1:75595813-75596162, human chr 1:75596706-75596947, human chr 1:75600674-75601415 and human chr 1:75601998-75602878.

[0076] In some implementations, the DNA region containing LHX8 is derived from the following defined regions: human chr1:75602387-75602574, human chr1:75602302-75602452, human chr1:75596706-75596856, and human chr1:75602336-75602486.

[0077] In some implementations, the DNA region containing RAB37 is derived from the region defined in human chr 17:72667195-72733545.

[0078] In some implementations, the DNA region containing RAB37 is derived from regions selected from the following groups: human chr17:72667195-72667748 and human chr 17:72732848-72733545.

[0079] In some implementations, the DNA region containing RAB37 is derived from the following defined regions: human chr17:72667311-72667431, human chr17:72667628-72667748, human chr17:72732948-72733098, and human chr17:72733298-72733448.

[0080] In some embodiments, the DNA region of the ZIC4 gene is derived from a region selected from the group consisting of: the region defined by human chr 3:147108512-147111703, the region defined by human chr 3:147113608-147114479, and the region defined by human chr 3:147115764-147116421.

[0081] In some embodiments, the DNA region of the ZIC4 gene includes the region defined by human chr3:147108512-147111703. In some embodiments, the DNA region of the ZIC4 gene includes the region defined by human chr3:147113608-147114479. In some embodiments, the DNA region of the ZIC4 gene includes the region defined by human chr3:147115764-147116421.

[0082] In some embodiments, the DNA region of the ZIC4 gene is derived from a region selected from the group consisting of: the region defined by human chr3:147109470-147109665, the region defined by human chr3:147110272-147110420, the region defined by human chr3:147110474-147110594, and the region defined by human chr3:147111574-147111694.

[0083] In some embodiments, the DNA region of the ZIC4 gene includes the region defined by human chr3:147109470-147109665. In some embodiments, the DNA region of the ZIC4 gene includes the region defined by human chr3:147110272-147110420. In some embodiments, the DNA region of the ZIC4 gene includes the region defined by human chr3:147110474-147110594. In some embodiments, the DNA region of the ZIC4 gene includes the region defined by human chr3:147111574-147111694.

[0084] In some implementations, the DNA region containing ZNF571-AS1 is derived from the region defined in human chr 19:38039561-38085811.

[0085] In some implementations, the DNA region containing ZNF571-AS1 is derived from regions selected from the following groups: human chr19:38039561-38040395, human chr 19:38042130-38042250 and human chr 19:38085148-38085811.

[0086] In some implementations, the DNA region containing ZNF571-AS1 is derived from the following defined regions: human chr19:38042130-38042250, human chr19:38039761-38039911, human chr19:38039891-38040041, and human chr19:38085348-38085498.

[0087] In some implementations, the DNA region containing TBX15 is derived from the region defined in human chr 1:119522385-119532196.

[0088] In some implementations, the DNA region containing TBX15 is derived from regions selected from the group defined below: human chr1:119522385-119522638, human chr1:119526782-119527192, human chr1:119529819-119530712 and human chr1:119531991-119532196.

[0089] In some implementations, the DNA region containing TBX15 is derived from the following defined regions: human chr1:119530462-119530649, human chr1:119522485-119522635, human chr1:119526782-119526932 and human chr1:119529819-119529969.

[0090] In some embodiments, the DNA region of the ZNF154 gene is derived from the region defined in human chr 19:58220090-58220517.

[0091] In some embodiments, the DNA region of the ZNF154 gene includes the region defined in human chr 19:58220090-58220517.

[0092] In some embodiments, the DNA region of the ZNF154 gene is derived from a region selected from the group consisting of: the region defined by human chr19:58220090-58220187, the region defined by human chr19:58220138-58220288, the region defined by human chr19:58220318-58220468, and the region defined by human chr19:58220333-58220483.

[0093] In some embodiments, the DNA region of the ZNF154 gene includes the region defined by human chr19:58220090-58220187. In some embodiments, the DNA region of the ZNF154 gene includes the region defined by human chr19:58220138-58220288. In some embodiments, the DNA region of the ZNF154 gene includes the region defined by human chr19:58220318-58220468. In some embodiments, the DNA region of the ZNF154 gene includes the region defined by human chr19:58220333-58220483.

[0094] In some implementations, the DNA region containing SEPT9 is derived from the region defined in human chr 17:75277318-75447821.

[0095] In some implementations, the DNA region containing SEPT9 is derived from regions selected from the group defined below: human chr17:75277318-75278172, human chr 17:75368688-75370506, human chr 17:75398284-75398527 and human chr17:75447477-75447821.

[0096] In some implementations, the DNA region containing SEPT9 is derived from the following defined regions: human chr17:75369509-75369659, human chr17:75277390-75277540, human chr17:75277537-75277687, and human chr17:75277944-75278094.

[0097] In some implementations, the DNA region containing ALX1 is derived from the region defined in human chr 12:85673198-85674700.

[0098] In some implementations, the DNA region containing ALX1 is derived from regions selected from the group defined below: human chr12:85673198-85673361 and human chr 12:85673878-85674700.

[0099] In some implementations, the DNA region containing ALX1 is derived from the following defined regions: human chr12:85673198-85673361, human chr12:85673878-85674028, human chr12:85673978-85674128, and human chr12:85674078-85674228.

[0100] In some embodiments, the DNA region of the KCNMA1 gene is derived from the region defined in human chr 10:79396095-79398495.

[0101] In some embodiments, the DNA region of the KCNMA1 gene includes the region defined in human chr 10:79396095-79398495.

[0102] In some embodiments, the DNA region of the KCNMA1 gene is derived from a region selected from the group consisting of: the region defined by human chr10:79396732-79396890, the region defined by human chr10:79396195-79396345, the region defined by human chr10:79396975-79397095, and the region defined by human chr10:79396595-79396745.

[0103] In some embodiments, the DNA region of the KCNMA1 gene includes the region defined by human chr10:79396732-79396890. In some embodiments, the DNA region of the KCNMA1 gene includes the region defined by human chr10:79396195-79396345. In some embodiments, the DNA region of the KCNMA1 gene includes the region defined by human chr10:79396975-79397095. In some embodiments, the DNA region of the KCNMA1 gene includes the region defined by human chr10:79396595-79396745.

[0104] In some embodiments, the DNA region of the LRRC9 gene is derived from the region defined in human chr 14:60386271-60386924. In some embodiments, the DNA region of the LRRC9 gene includes the region defined in human chr 14:60386271-60386924.

[0105] In some embodiments, the DNA region of the LRRC9 gene is derived from a region selected from the group consisting of: the region defined by human chr14:60386371-60386521, the region defined by human chr14:60386572-60386722, the region defined by human chr14:60386670-60386820, and the region defined by human chr14:60386774-60386924.

[0106] In some embodiments, the DNA region of the LRRC9 gene includes the region defined by human chr14:60386371-60386521. In some embodiments, the DNA region of the LRRC9 gene includes the region defined by human chr14:60386572-60386722. In some embodiments, the DNA region of the LRRC9 gene includes the region defined by human chr14:60386670-60386820. In some embodiments, the DNA region of the LRRC9 gene includes the region defined by human chr14:60386774-60386924.

[0107] In some embodiments, the DNA region of the FOXF2 gene is derived from a region selected from the group consisting of: the region defined by human chr6:1381743-1385211, the region defined by human chr6:1389139-1391393, and the region defined by chr6:1392975-1394170.

[0108] In some embodiments, the DNA region of the FOXF2 gene includes the region defined by human chr6:1381743-1385211. In some embodiments, the DNA region of the FOXF2 gene includes the region defined by human chr6:1389139-1391393. In some embodiments, the DNA region of the FOXF2 gene includes the region defined by human chr6:1392975-1394170.

[0109] In some embodiments, the DNA region of the FOXF2 gene is derived from a region selected from the group consisting of: the region defined by human chr6:1384539-1384689, the region defined by human chr6:1392975-1393095, the region defined by human chr6:1394011-1394131, and the region defined by human chr6:1393647-1393767.

[0110] In some embodiments, the DNA region of the FOXF2 gene includes the region defined by human chr6:1384539-1384689. In some embodiments, the DNA region of the FOXF2 gene includes the region defined by human chr6:1392975-1393095. In some embodiments, the DNA region of the FOXF2 gene includes the region defined by human chr6:1394011-1394131. In some embodiments, the DNA region of the FOXF2 gene includes the region defined by human chr6:1393647-1393767.

[0111] In some embodiments, the method includes providing reagents capable of recognizing and / or binding to the DNA region containing the target gene, its base-transformed region, or its complementary region.

[0112] In some embodiments, the reagent comprises a nucleic acid molecule capable of recognizing and / or binding to the DNA region containing the target gene, its base-transformed region, or its complementary region.

[0113] In some embodiments, the reagent comprises primers and / or probes capable of recognizing and / or binding to the DNA region containing the target gene, its base-transformed region, or its complementary region.

[0114] In some implementations, the DNA region comprises one, two, three, four or more DNA regions.

[0115] In some implementations, the method includes obtaining nucleic acids from the sample.

[0116] In some embodiments, the method includes transforming the nucleic acid such that methylated bases and unmethylated bases form different substances after transformation.

[0117] In some embodiments, the transformation includes transformation by a deamination reagent and / or a methylation-sensitive restriction endonuclease.

[0118] In some embodiments, the method includes contacting the sample with a nucleic acid molecule or fragment thereof capable of recognizing, amplifying, and / or binding to the DNA region containing the target gene, its base-transformed region, its complementary region, or a region thereof. For example, the DNA region fragment is a fragment of 50-300 bp in length. Alternatively, the DNA region fragment is a fragment of 100-200 bp in length.

[0119] In some embodiments, the method for determining the methylation state includes determining the presence and / or content of a substance formed after the conversion by a base having a methylation state.

[0120] In some embodiments, the method for determining methylation status includes determining the presence and / or content of DNA regions or fragments thereof that have a methylation status.

[0121] In some embodiments, the presence and / or quantity of the DNA region or fragment thereof having the modified state is determined by the fluorescence Ct value detected by the fluorescence PCR method.

[0122] In some implementations, the presence of urothelial carcinoma, or the risk of urothelial carcinoma formation, is determined by confirming the presence of a modification state of the DNA region or fragment thereof and / or the amount of the DNA region or fragment thereof having a higher level of modification state relative to a reference level.

[0123] In some embodiments, the method further includes amplifying the DNA region or fragment thereof in the sample to be tested before determining the presence and / or content of modifications to the DNA region or fragment thereof.

[0124] In some implementations, the amplification includes PCR amplification.

[0125] In some embodiments, the method further includes: if one or more methylation states of the target gene in the sample are detected to be positive, the test result is considered positive; if all methylation states of the target gene in the sample are detected to be negative, the test result is considered negative.

[0126] In some embodiments, the product further comprises a reagent that selectively modifies unmethylated cytosine residues in the DNA of the sample to produce detectable modified residues, but the reagent does not modify methylated cytosine residues.

[0127] In some embodiments, the product further comprises a deamination agent and / or a methylation-sensitive restriction endonuclease.

[0128] In some embodiments, the deamination agent comprises a bisulfite.

[0129] In some implementations, the product is a reagent kit.

[0130] In some embodiments, this application provides primers selected from any of the following groups for use in the methods, products, and / or kits of this application: (1) SEQ ID NO:1 and SEQ ID NO:109. (2) SEQ ID NO:2 and SEQ ID NO:110. (3) SEQ ID NO:3 and SEQ ID NO:111. (4) SEQ ID NO:4 and SEQ ID NO:112. (5) SEQ ID NO:5 and SEQ ID NO:113. (6) SEQ ID NO:6 and SEQ ID NO:114. (7) SEQ ID NO:7 and SEQ ID NO:115. (8) SEQ ID NO:8 and SEQ ID NO:116. (9) SEQ ID NO:9 and SEQ ID NO:117. (10) SEQ ID NO:10 and SEQ ID NO:118. (11) SEQ ID NO:11 and SEQ ID NO:119. (12) SEQ ID NO:12 and SEQ ID NO:120. (13) SEQ ID NO:13 and SEQ ID NO:121. (14) SEQ ID NO:14 and SEQ ID NO:122. (15) SEQ ID NO:15 and SEQ ID NO:123. (16) SEQ ID NO:16 and SEQ ID NO:124. (17) SEQ ID NO:17 and SEQ ID NO:125. (18) SEQ ID NO:18 and SEQ ID NO:126. (19) SEQ ID NO:19 and SEQ ID NO:127. (20) SEQ ID NO:20 and SEQ ID NO:128. (21) SEQ ID NO:21 and SEQ ID NO:129. (22) SEQ ID NO:22 and SEQ ID NO:130. (23) SEQ ID NO:23 and SEQ ID NO:131. (24) SEQ ID NO:24 and SEQ ID NO:132. (25) SEQ ID NO:25 and SEQ ID NO:133. (26) SEQ ID NO:26 and SEQ ID NO:134. (27) SEQ ID NO:27 and SEQ ID NO:135. (28) SEQ ID NO:28 and SEQ ID NO:136. (29) SEQ ID NO:29 and SEQ ID NO:137. (30) SEQ ID NO:30 and SEQ ID NO:138. (31) SEQ ID NO:31 and SEQ ID NO:139.(32) SEQ ID NO:32 and SEQ ID NO:140. (33) SEQ ID NO:33 and SEQ ID NO:141. (34) SEQ ID NO:34 and SEQ ID NO:142. (35) SEQ ID NO:35 and SEQ ID NO:143. (36) SEQ ID NO:36 and SEQ ID NO:144. (37) SEQ ID NO:37 and SEQ ID NO:145. (38) SEQ ID NO:38 and SEQ ID NO:146. (39) SEQ ID NO:39 and SEQ ID NO:147. (40) SEQ ID NO:40 and SEQ ID NO:148. (41) SEQ ID NO:41 and SEQ ID NO:149. (42) SEQ ID NO:42 and SEQ ID NO:150. (43) SEQ ID NO:43 and SEQ ID NO:151. (44) SEQ ID NO:44 and SEQ ID NO:152. (45) SEQ ID NO:45 and SEQ ID NO:153. (46) SEQ ID NO:46 and SEQ ID NO:154. (47) SEQ ID NO:47 and SEQ ID NO:155. (48) SEQ ID NO:48 and SEQ ID NO:156. (49) SEQ ID NO:49 and SEQ ID NO:157. (50) SEQ ID NO:50 and SEQ ID NO:158. (51) SEQ ID NO:51 and SEQ ID NO:159. (52) SEQ ID NO:52 and SEQ ID NO:160. (53) SEQ ID NO:53 and SEQ ID NO:161. (54) SEQ ID NO:54 and SEQ ID NO:162. (55) SEQ ID NO:55 and SEQ ID NO:163. (56) SEQ ID NO:56 and SEQ ID NO:164. (57) SEQ ID NO:57 and SEQ ID NO:165. (58) SEQ ID NO:58 and SEQ ID NO:166. (59) SEQ ID NO:59 and SEQ ID NO:167. (60) SEQ ID NO:60 and SEQ ID NO:168. (61) SEQ ID NO:61 and SEQ ID NO:169. (62) SEQ ID NO:62 and SEQ ID NO:170. (63) SEQ ID NO:63 and SEQ ID NO:171.(64) SEQ ID NO:64 and SEQ ID NO:172. (65) SEQ ID NO:65 and SEQ ID NO:173. (66) SEQ ID NO:66 and SEQ ID NO:174. (67) SEQ ID NO:67 and SEQ ID NO:175. (68) SEQ ID NO:68 and SEQ ID NO:176. (69) SEQ ID NO:69 and SEQ ID NO:177. (70) SEQ ID NO:70 and SEQ ID NO:178. (71) SEQ ID NO:71 and SEQ ID NO:179. (72) SEQ ID NO:72 and SEQ ID NO:180. (73) SEQ ID NO:73 and SEQ ID NO:181. (74) SEQ ID NO:74 and SEQ ID NO:182. (75) SEQ ID NO:75 and SEQ ID NO:183. (76) SEQ ID NO:76 and SEQ ID NO:184. (77) SEQ ID NO:77 and SEQ ID NO:185. (78) SEQ ID NO:78 and SEQ ID NO:186. (79) SEQ ID NO:79 and SEQ ID NO:187. (80) SEQ ID NO:80 and SEQ ID NO:188. (81) SEQ ID NO:81 and SEQ ID NO:189. (82) SEQ ID NO:82 and SEQ ID NO:190. (83) SEQ ID NO:83 and SEQ ID NO:191. (84) SEQ ID NO:84 and SEQ ID NO:192. (85) SEQ ID NO:85 and SEQ ID NO:193. (86) SEQ ID NO:86 and SEQ ID NO:194. (87) SEQ ID NO:87 and SEQ ID NO:195. (88) SEQ ID NO:88 and SEQ ID NO:196. (89) SEQ ID NO:89 and SEQ ID NO:197. (90) SEQ ID NO:90 and SEQ ID NO:198. (91) SEQ ID NO:91 and SEQ ID NO:199. (92) SEQ ID NO:92 and SEQ ID NO:200. (93) SEQ ID NO:93 and SEQ ID NO:201. (94) SEQ ID NO:94 and SEQ ID NO:202. (95) SEQ ID NO:95 and SEQ ID NO:203.(96) SEQ ID NO:96 and SEQ ID NO:204. (97) SEQ ID NO:97 and SEQ ID NO:205. (98) SEQ ID NO:98 and SEQ ID NO:206. (99) SEQ ID NO:99 and SEQ ID NO:207. (100) SEQ ID NO:100 and SEQ ID NO:208. (101) SEQ ID NO:101 and SEQ ID NO:209. (102) SEQ ID NO:102 and SEQ ID NO:210. (103) SEQ ID NO:103 and SEQ ID NO:211. (104) SEQ ID NO:104 and SEQ ID NO:212. (105) SEQ ID NO:105 and SEQ ID NO:213. (106) SEQ ID NO:106 and SEQ ID NO:214. (107) SEQ ID NO:107 and SEQ ID NO:215. (108)SEQ ID NO:108 and SEQ ID NO:216.

[0131] In some embodiments, this application provides probes selected from any one of the sequences in SEQ ID NO:217-324 for use in the methods, products and / or kits of this application.

[0132] Other aspects and advantages of this application will readily be apparent to those skilled in the art from the detailed description below. Only exemplary embodiments of this application are shown and described in the following detailed description. As will be appreciated by those skilled in the art, the content of this application enables them to make modifications to the disclosed specific embodiments without departing from the spirit and scope of the invention to which this application pertains. Accordingly, the descriptions in the accompanying drawings and specification of this application are merely exemplary and not restrictive. Attached Figure Description

[0133] The specific features of the invention involved in this application are shown in the appended claims. The features and advantages of the invention can be better understood by referring to the exemplary embodiments and drawings described in detail below. A brief description of the drawings is as follows:

[0134] Figures 1 to 15The NGS sequencing results show the methylation levels of the HOXA9, KCNQ1DN, SIM2, VIM, ZIC4, ZNF154, KCNMA1, FOXF2, PAX1, BCL11B, LHX8, MARCH11, RAB37, TBX15, and ZNF571-AS1 genes in newly diagnosed and follow-up samples of urothelial carcinoma. Among them, TD1, TD2...TD10 are newly diagnosed positive samples, ND1, ND2...ND10 are newly diagnosed negative samples, TR1, TR2...TR10 are follow-up positive samples, and NR1, NR2...NR10 are follow-up negative samples. Detailed Implementation

[0135] The following specific embodiments illustrate the implementation of the invention. Those skilled in the art can easily understand other advantages and effects of the invention from the content disclosed in this specification.

[0136] In this application, the term "HOXA9" generally refers to the gene encoding the homeobox protein Hox-A9, also known as HOX1 or HOX1G. The human HOXA9 gene is located in Chromosome 7:27,202,054-27,210,117 on the reverse strand (GRCh37:CM000669.1). More information about the human HOXA9 gene can be found in ensemble number ENSG00000078399.

[0137] In this application, the term "VIM" generally refers to the gene encoding vimentin, also known as CTRCT30 or HEL113. The human VIM gene is located in Chromosome 10:17,270,258-17,279,592 on the forward strand (GRCh37:CM000672.1). More information about the human VIM gene can be found in ensemble number ENSG00000026025.

[0138] In this application, the term "SIM2" generally refers to the gene encoding the SIM bHLH transcription factor 2, also known as BHLHE15, MGC119447, or SIM. The human SIM2 gene is located in Chromosome 21:38,071,433-38,122,218 on the forward strand (GRCh37:CM000683.1). More information about the human SIM2 gene can be found in ensemble number NSG00000159263.

[0139] In this application, the term "ZIC4" generally refers to the gene encoding member 4 of the Zic family. The human ZIC4 gene is located in Chromosome 3:147,103,833-147,124,647 on the reverse strand (GRCh37:CM000665.1). More information about the human ZIC4 gene can be found in ensemble number NSG00000174963.

[0140] In this application, the term "ZNF154" generally refers to the gene encoding zinc finger protein 154, also known as PHZ-92. The human ZNF154 gene is located in Chromosome 19:58,208,735-58,220,579 on the reverse strand (GRCh37:CM000681.1). More information about the human ZNF154 gene can be found in ensemble number ENSG00000179909.

[0141] In this application, the term "KCNMA1" generally refers to the potassium-conducting calcium-activated channel, subfamily M, α member 1, also known as KCNMA. The human KCNMA1 gene is located in Chromosome 10:78,629,359-79,398,353 on the reverse strand (GRCh37:CM000672.1). More information about the human KCNMA1 gene can be found in ensemble number ENSG00000156113.

[0142] In this application, the term "FOXF2" generally refers to the gene encoding the forkhead box protein F2, also known as FKHL6, FREAC-2, or FREAC2. The human FOXF2 gene is located on the forward strand of Chromosome 6:1,390,069-1,395,832 (GRCh37:CM000668.1). More information about the human FOXF2 gene can be found in ensemble number ENSG00000137273. Additionally, the FOXF2 gene contains a branched transcript, FOXF2-DT. The human FOXF2-DT transcript is located on the reverse strand of Chromosome 6:1,384,025-1,385,301. More information about the human FOXF2-DT gene can be found in ensemble number ENSG00000261730.

[0143] In this application, the term "TRPS1" generally refers to the gene encoding the transcriptional repressor GATA-binding 1, also known as GC79 or LGCR. The human TRPS1 gene is located in Chromosome 8:116,420,724-116,821,899 on the reverse strand (GRCh37:CM000670.1). More information about the human TRPS1 gene can be found in ensemble number ENSG00000104447.

[0144] In this application, the term "RAB37" generally refers to RAB37, a member of the RAS oncogene family. The human RAB37 gene is located in Chromosome 17:72,666,717-72,743,474 on the forward strand (GRCh37:CM000679.1). More information about the human RAB37 gene can be found in ensemble number ENSG00000172794.

[0145] In this application, the term "SEPT9" generally refers to the gene encoding the Septin9 protein, and may also be referred to as AF17Q25, KIAA0991, MSF, MSF1, PNUTL4, SEPTIN9, or SEPTD1. The human SEPT9 gene is located in Chromosome 17:75,276,651-75,496,678 on the forward strand (GRCh37:CM000679.1). More information about the human SEPT9 gene can be found in ensemble number ENSG00000184640.

[0146] In this application, the term "LHX8" generally refers to the gene encoding LIM homeobox protein 8, also known as LHX7. The human LHX8 gene is located in Chromosome 1:75,594,119-75,627,218 on the forward strand (GRCh37:CM000663.1). More information about the human LHX8 gene can be found in ensemble number ENSG00000162624.

[0147] In this application, the term "NKX6-2" generally refers to the gene encoding the homeobox protein Nkx-6.2, and may also be referred to as GTX, NKX6.1, or NKX6B. The human NKX6-2 gene is located in Chromosome 10:134,598,297-134,599,556 on the reverse strand (GRCh37:CM000672.1). More information about the human NKX6-2 gene can be found in ensemble number ENSG00000148826.

[0148] In this application, the term "ZNF571-AS1" generally refers to antisense RNA 1 of the ZNF571 gene. The human ZNF571-AS1 gene is located in Chromosome 19:38,039,816-38,078,249 on the forward strand (GRCh37:CM000681.1). More information about the human ZNF571-AS1 gene can be found in ensemble number ENSG00000267470.

[0149] In this application, the term "NKX1-1" generally refers to the gene encoding NK1 homeobox protein 1, also known as HSPX153 or SAX2. The human NKX1-1 gene is located in Chromosome 4:1,396,720-1,400,119 on the reverse strand (GRCh37:CM000666.1). More information about the human NKX1-1 gene can be found in ensemble number ENSG00000235608.

[0150] In this application, the term "MARCH11" generally refers to the gene encoding membrane-associated ring finger protein antibody 11, also known as MARCH-XI. The human MARCH11 gene is located in Chromosome 5:16,067,248-16,180,871 on the reverse strand (GRCh37:CM000667.1). More information about the human MARCH11 gene can be found in ensemble number ENSG00000183654.

[0151] In this application, the term "PENK" generally refers to the gene encoding the endogenous hormone proenkephalin. The human PENK gene is located in Chromosome 8:57,349,233-57,359,293 on the reverse strand (GRCh37:CM000670.1). More information about the human PENK gene can be found in ensemble number ENSG00000181195.

[0152] In this application, the term "TBX15" generally refers to a transcription factor in the T-box that plays a crucial role in embryonic development. The human TBX15 gene is located in Chromosome 1:119,425,669-119,532,179 on the reverse strand (GRCh37:CM000663.1). More information about the human TBX15 gene can be found in ensemble number ENSG00000092607.

[0153] In this application, the term "NKX2-8" generally refers to the gene encoding NK2 homeobox protein 8, and may also be referred to as NKX2-9, NKX2.8, and NKX2H. The human NKX2-8 gene is located in Chromosome 14:37,049,784-37,051,812 on the reverse strand (GRCh37:CM000676.1). More information about the human NKX2-8 gene can be found in ensemble number ENSG00000136327.

[0154] In this application, the term "NRN1" generally refers to the gene encoding the neurotrophic factor neuroitin, also known as NRN. The human NRN1 gene is located in Chromosome 6:5,998,232-6,007,200 on the reverse strand (GRCh37:CM000668.1). More information about the human NRN1 gene can be found in ensemble number ENSG000000124785.

[0155] In this application, the term "BCL11B" generally refers to a gene encoding a zinc finger protein, also known as ZNF856B or ATL1. The human BCL11B gene is located in Chromosome 14:99,635,624-99,737,861 on the reverse strand (GRCh37:CM000676.1). More information about the human BCL11B gene can be found in ensemble number ENSG00000127152.

[0156] In this application, the term "ALX1" generally refers to the gene encoding ALX homeobox protein 1, also known as CART1, FND3, or HEL23. The human ALX1 gene is located in Chromosome 12:85,673,885-85,695,562 on the forward strand (GRCh37:CM000674.1). More information about the human ALX1 gene can be found in ensemble number ENSG00000180318.

[0157] In this application, the term "KCNQ1DN" generally refers to the downstream neighbor gene of KCNQ1, also known as BWRT or HSA404617. The human KCNQ1DN gene is located in Chromosome 11:2,891,263-2,893,335 on the forward strand (GRCh37:CM000673.1). More information about the human KCNQ1DN gene can be found in ensemble number NSG00000237941.

[0158] In this application, the term "LRRC9" generally refers to the gene rich in leucine repeat sequence 9, also known as FLJ46156. The human LRRC9 gene is located in Chromosome 14:60,386,431-60,530,277 on the forward strand (GRCh37:CM000676.1). More information about the human LRRC9 gene can be found in ensemble number ENSG00000131951.

[0159] In this application, the term "BNC1" generally refers to the gene encoding basic nucleoprotein 1, also known as BNC, BSN1, or HsT19447. The human BNC1 gene is located in Chromosome 15:83,924,655-83,953,466 on the reverse strand (GRCh37:CM000677.1). More information about the human BNC1 gene can be found in ensemble number ENSG00000169594.

[0160] In this application, the term "IRX4" generally refers to the gene encoding iroquois homeobox protein 4, also known as IRXA3. The human IRX4 gene is located in Chromosome 5:1,877,541-1,887,350 in the reverse strand (GRCh37:CM000667.1). More information about the human IRX4 gene can be found in ensemble number ENSG00000113430.

[0161] In this application, the term "SLC26A4" generally refers to member 4 of solute carrier family 26 (anion exchanger), also known as DFNB4, EVA, PDS, or TDH2B. The human SLC26A4 gene is located in Chromosome 7:107,301,080-107,358,254 (GRCh37:CM000669.1). More information about the human SLC26A4 gene can be found in ensemble number ENSG00000091137.

[0162] In this application, the term "PAX1" generally refers to PAX1 in the PAX family gene, also known as HUP48, OFC2. The human PAX1 gene is located in Chromosome 20:21,686,297-21,696,620 in the forward strand (GRCh37:CM000682.1). More information about the human PAX1 gene can be found in ensemble number ENSG00000125813.

[0163] The genes in this application can be described by their names and their chromosomal coordinates.

[0164] In this application, the term "sample" or "sample to be tested" generally refers to a sample that needs to be tested. For example, it can be used to detect whether one or more gene regions on the sample to be tested are modified.

[0165] In this application, the term "healthy population" generally refers to a population whose tumors are currently undetectable by tumor detection methods. A healthy population can develop into a cancer patient, a high-risk group for cancer, a suspected cancer patient, or a cancer-susceptible population. "Healthy population" is a concept relative to "cancer patient," which typically refers to a population confirmed to have a tumor through pathological diagnosis or other testing methods.

[0166] In this application, the term "high-risk population for cancer" generally refers to a population with a higher risk of developing cancer. For example, people with a family history of cancer, people who carry cancer susceptibility genes, people with unhealthy lifestyles (such as smokers, alcoholics, and those who frequently stay up late), and people who are frequently exposed to toxic or harmful substances in their living or working environment.

[0167] In this application, the term "suspected cancer patient" generally refers to a group of people who have cancer symptoms but have not been confirmed to have cancer by pathological diagnosis or other testing methods.

[0168] In this application, the term "tumor-susceptible population" generally refers to a population that is prone to developing tumors. For example, people with a family history of cancer, people who carry tumor susceptibility genes, people with unhealthy lifestyles (such as smokers, alcoholics, and those who frequently stay up late), and people who are frequently exposed to toxic or harmful substances in their living or working environment.

[0169] For urothelial carcinoma, such as bladder cancer, the susceptible population or high-risk population may include, but is not limited to, the following groups: patients with unclear clinical diagnosis, high-risk populations for urothelial carcinoma (age > 50 years; long-term smokers; people with long-term exposure to carcinogens such as industrial chemicals; people with a family history of bladder cancer; and people who ingest aristolochic acid-containing traditional Chinese medicine).

[0170] In this application, the term "DNA region" generally refers to a sequence of two or more covalently bonded naturally occurring or modified deoxyribonucleotides. For example, a DNA region of a gene can refer to the location of a specific deoxyribonucleotide sequence in which the gene is located, such as a sequence that encodes the gene. For example, the DNA region of this application includes the full length of the DNA region, its complementary region, or fragments thereof. For example, a sequence of at least about 20 kb upstream and downstream of the detection region provided in this application can be used as the detection region. For example, a sequence of at least about 20 kb, at least about 15 kb, at least about 10 kb, at least about 5 kb, at least about 3 kb, at least about 2 kb, at least about 1 kb, or at least about 0.5 kb upstream and downstream of the detection region provided in this application can be used as the detection region. For example, suitable primers and probes can be designed based on the region for the detection of sample methylation. In this application, when referring to a DNA region, unless otherwise specified, it generally refers to the Human Reference Genome Version GRCh37, UCSC version number hg19.

[0171] In this application, the term "methylation" generally refers to the methylation state of a gene fragment, nucleotide, or base thereof in this application. For example, the DNA fragment containing the gene in this application may be methylated on one or more strands. For example, the DNA fragment containing the gene in this application may be methylated at one or more sites. For example, methylation may refer to the conversion of the 5′ cytosine of a CpG island dinucleotide on a DNA sequence to 5′ methylcytosine (5mC), and may also include the formation of N6-methylpurine (N6-mA) and 7-methylguanine (7-mG). CpG dinucleotides are typically concentrated in the promoter region and exons of human genes. In this application, the term "methylation state" generally refers to the methylation state of one or more nucleotides or bases in the DNA region containing the gene. For example, the methylation state of the DNA region containing the gene may be the methylation state of one or more CpG dinucleotides within that DNA region.

[0172] For example, "methylation state" can refer to the presence or absence of 5-methylcytosine ("5-mC" or "5-mCyt") at one or more CpG dinucleotides within a DNA sequence. The methylation state at one or more specific CpG methylation sites (each with two CpG dinucleotide sequences) within a DNA sequence includes "unmethylated," "fully methylated," and "hemimethylated." The term "hemimethylated" can refer to the methylation state of double-stranded DNA where only one strand is methylated. The term "hypermethylation" can refer to an average methylation state corresponding to an increased presence of 5-mCyt at one or more CpG dinucleotides in the DNA sequence of the test DNA sample compared to the amount of 5-mCyt found at the corresponding CpG dinucleotide in a normal control DNA sample. The term "hypomethylation" can refer to an average methylation state corresponding to a decreased presence of 5-mCyt at one or more CpG dinucleotides in the DNA sequence of the test DNA sample compared to the amount of 5-mCyt found at the corresponding CpG dinucleotide in a normal control DNA sample.

[0173] For example, the methylation state in this application can refer to the presence, absence, and / or content of methylation at one or more specific nucleotides within a DNA region. For example, the methylation state in this application can refer to the methylation state of each base or each specific base (e.g., cytosine) in a specific DNA sequence. For example, the methylation state in this application can refer to the methylation state of base pair combinations and / or base combinations in a specific DNA sequence. For example, the methylation state in this application can refer to information about the regional methylation density in a specific DNA sequence (including the DNA region containing the gene or a specific regional segment thereof), without providing precise location information of where methylation occurs within the sequence.

[0174] For example, having or having a high methylation state can be associated with transcriptional silencing of a specific region. For example, having or having a high methylation state can be associated with the ability to be transformed by methylation-specific transforming agents (e.g., deaminating agents and / or methylation-sensitive restriction enzymes). For example, transformation can refer to being converted into other substances and / or being cleaved or digested.

[0175] In this application, the term "transformation" generally refers to the conversion of one or more structures into another. For example, the transformations in this application can be specific. For example, cytosine without methylation modification can be transformed into other structures (e.g., uracil), while cytosine with methylation modification can remain substantially unchanged after transformation. For example, cytosine without methylation modification can be cleaved after transformation, while cytosine with methylation modification can remain substantially unchanged after transformation.

[0176] In this application, the term "deamination agent" generally refers to a substance capable of removing an amino group. For example, a deamination agent can remove the amino group from unmodified cytosine.

[0177] In this application, the term "bisulfite" generally refers to a reagent capable of distinguishing between modified and unmodified DNA regions. For example, a bisulfite may include bisulfite, its analogues, or combinations thereof. For example, a bisulfite can deamination of the amino group of unmodified cytosine to distinguish it from modified cytosine. In this application, the term "analyte" generally refers to a substance having a similar structure and / or function. For example, an analogue of a bisulfite may have a similar structure to a bisulfite. For example, an analogue of a bisulfite may refer to a reagent that can also distinguish between modified and unmodified DNA regions.

[0178] In this application, the term "methylation-sensitive restriction endonuclease" generally refers to an enzyme that selectively digests nucleic acids based on the methylation state of its recognition site. For example, for a restriction endonuclease that specifically cleaves when the recognition site is unmethylated, cleavage may not occur or may occur with significantly reduced efficiency when the recognition site is methylated. For instance, a methylation-specific restriction endonuclease may recognize sequences containing CG dinucleotides (e.g., cgcg or cccggg).

[0179] In this application, the term "primer" can be a natural or synthetic oligonucleotide that, upon forming a double helix with a polynucleotide template, can act as a starting point for nucleic acid synthesis and extend from its 3' end along the template to form an extended double helix. The sequence of the nucleotides added during the extension process is determined by the sequence of the template polynucleotide. Primers can typically be extended using polymerases such as nucleic acid polymerases.

[0180] In this application, the term "complementary" can include hybridization or base pairing or duplex formation between nucleotides or nucleic acids, such as between the two strands of a double-stranded DNA molecule, or between primer binding sites on oligonucleotide primers and single-stranded nucleic acids. Complementary nucleotides can typically be A and T (or A and U) or C and G. For two single-stranded RNA or DNA molecules, they can be considered substantially complementary when the nucleotides of one strand pair with at least about 80% (typically at least about 90% to about 95%, or even about 98% to about 100%) of the other strand in optimal alignment and comparison and with appropriate nucleotide insertions or deletions. In one aspect, two complementary nucleotide sequences are capable of hybridization and can have less than 25% mismatch between the reversed nucleotides, more likely less than 15% mismatch, less than 5% mismatch, or no mismatch. For example, the two molecules can hybridize under highly stringent conditions.

[0181] In this application, the term "urothelial carcinoma" generally refers to cancer associated with the malignant proliferation of urothelial cells. Urothelial carcinoma can include upper urinary tract urothelial carcinoma (UTUC, e.g., renal pelvis cancer and ureteral cancer) and lower urinary tract urothelial carcinoma (e.g., bladder cancer). Urothelial carcinoma can occur in the renal pelvis, ureter, or bladder.

[0182] In this application, the term "progression" generally refers to a change in a disease from a less severe state to a more severe state. For example, tumor progression can include an increase in the number or severity of the tumor, the extent of cancer cell metastasis, or the rate of cancer growth or spread. For example, tumor progression can include the stages of this cancer from a less severe state to a more severe state, such as progression from stage I to stage II, from stage II to stage III, etc.

[0183] In this application, the term "occurrence" generally refers to the presence of a lesion in an individual's body. For example, when a tumor occurs, the individual can be diagnosed as a cancer patient.

[0184] In this application, the term "fluorescent PCR" generally refers to a quantitative or semi-quantitative PCR technique. For example, it may be a real-time quantitative polymerase chain reaction (PCR), a quantitative polymerase chain reaction (PCR), or a kinetic polymerase chain reaction (PCR). For instance, PCR can be used to amplify and quantitatively detect the amount of the initial target nucleic acid using an intercalating fluorescent dye or a sequence-specific probe, which may contain a fluorescent reporter molecule that is detectable only by hybridization with the target nucleic acid.

[0185] In this application, the term "PCR amplification" generally refers to a polymerase chain amplification reaction. For example, PCR amplification in this application may include any polymerase chain amplification reaction currently known for DNA amplification.

[0186] In this application, the term "fluorescence Ct value" generally refers to a measurement for quantitative or semi-quantitative evaluation of target nucleic acids. For example, it may refer to the number of amplification reaction cycles required for the fluorescence signal to reach a set threshold.

[0187] In this application, the term "comprising" generally indicates that a scope or element includes other elements or features. This is used to define the scope of a particular technology, making it more flexible and inclusive. When the word "comprising" is used, it means that the scheme includes not only the explicitly listed elements but also other related elements or features to achieve similar functionality.

[0188] In this application, the term "and / or" is generally used to indicate a range of choices, representing an inclusive relationship between two or more elements or conditions. When "and / or" is used, it allows one or both of these elements or conditions to be present to achieve the same technical effect. For example, if the description contains an expression such as "A and / or B," it means that it can include either A or B, or both A and B. Invention Details

[0190] This application provides a combination of gene markers that can be used for the diagnostic detection of tumors, confirming the presence of a tumor, assessing the risk of tumor development, and / or evaluating the prognosis / progress of a tumor. Therefore, this application provides a method for the diagnostic detection of tumors, confirming the presence of a tumor, assessing the risk of tumor development, and / or evaluating the prognosis / progress of ...

Claims

1. The use of a reagent capable of determining the methylation status of the DNA region containing a target gene, its base-transformed region, or its complementary region in a sample, in the preparation of a urothelial carcinoma detection product, wherein the target gene is selected from the group consisting of: SIM2, ZIC4, ZNF154, VIM, SEPT9, NRN1, PENK, NKX6-2, NKX1-1, NKX2-8, TRPS1, ALX1, LRRC9, BNC1, IRX4, SLC26A4, and PAX1. The DNA region containing the SIM2 gene is derived from the region defined in the following group: human chr21:38068194-38120742. The DNA region containing the ZIC4 gene originates from the region defined in human chr 3:147108512-147116421. The DNA region containing the ZNF154 gene originates from the region defined in human chr 19:58220090-58220517, and the DNA region containing the VIM gene originates from the region defined in human chr10:17270431-17272617. The DNA region containing the SEPT9 gene originates from the region defined in human chr 17:75277318-75447821. The DNA region containing the NRN1 gene originates from the region defined in human chr 6:5999149-6009299. The DNA region containing the PENK gene can originate from the region defined in human chr 8:57358127-57360815, the DNA region containing the NKX6-2 gene can originate from the region defined in human chr 10:134597358-134602649, and the DNA region containing the NKX1-1 gene can originate from the region defined in human chr 4:1396292-1402435. The DNA region containing the NKX2-8 gene originates from the region defined in human chr 14:37049334-37053690; the DNA region containing the TRPS1 gene originates from the region defined in human chr 8:116660432-116681623; the DNA region containing the ALX1 gene may originate from the region defined in human chr 12:85673198-85674700; the DNA region containing the LRRC9 gene originates from the region defined in human chr14:60386271-60386924; and the DNA region containing the BNC1 gene originates from the region defined in human chr15:83951980-83953930. The DNA region containing the IRX4 gene originates from the region defined in human chr5:1874907-1887743. The DNA region containing SLC26A4 originates from the region defined in human chr7:107301205-107302416. The DNA region containing the PAX1 gene originates from the region defined in human chr 20:21683744-21695344.

2. The use according to claim 1, wherein the sample is from cancer patients, suspected cancer patients, cancer-susceptible populations, cancer-high-risk populations, or healthy populations.

3. The use according to claim 2, wherein the tumor patient includes patients who have not received anticancer therapy and / or patients who have received anticancer therapy.

4. The use according to claim 1, wherein the sample comprises tissue, cells and / or body fluids, preferably urine.

5. The use according to claim 1, wherein the reagent comprises a nucleic acid molecule capable of recognizing, amplifying, and / or binding to the DNA region containing the target gene, its base-transformed region, or its complementary region.

6. The use according to claim 1, wherein the reagent comprises primers and / or probes capable of recognizing, amplifying and / or binding to the DNA region containing the target gene, its base-transformed region or its complementary region.

7. The use according to claim 1, wherein the reagent further comprises a reagent that selectively modifies unmethylated cytosine residues in the DNA of the sample to produce detectable modified residues, but the reagent does not modify methylated cytosine residues.

8. The use according to claim 1, wherein the reagent further comprises a deamination agent and / or a methylation-sensitive restriction endonuclease.

9. The use according to claim 8, wherein the deamination agent comprises a bisulfite.

10. The use according to claim 1, wherein the product is a reagent kit.

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