Mixed exosome whitening gel as well as preparation method and application thereof
By loading milk, ginseng, and centella asiatica exosomes into a ternary composite gel network, the problem of low transdermal absorption rate of traditional whitening skin care products is solved, realizing the intelligent and targeted release of exosomes in the dermis, significantly inhibiting melanin production, and exhibiting good whitening and anti-aging effects.
Patent Information
- Application Number
- CN202511328255.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-09-17
- Publication Date
- 2025-11-18
AI Technical Summary
Existing whitening skincare products have difficulty penetrating the stratum corneum to reach the basal layer, resulting in poor melanin inhibition effects, and traditional ingredients have low transdermal absorption rates.
Milk, ginseng, and centella asiatica exosomes are loaded into a ternary composite gel network to form an intelligent responsive synergistic system, enabling intelligent targeted release of exosomes in the dermis.
Exosomes penetrate the skin barrier directly to the deep epidermis, effectively inhibiting melanin production, improving pigmentation and uneven skin tone, and have good whitening and anti-aging effects. They are highly biocompatible and suitable for sensitive skin.
Smart Images
Figure CN120960119A_ABST
Abstract
Description
Technical Field
[0001] This invention belongs to the field of skin care technology, specifically relating to a mixed exosome whitening gel, its preparation method, and its application. Background Technology
[0002] Melanin is the primary pigment affecting skin whiteness, and skincare products mainly target melanin production to achieve a whitening effect. Melanocytes reside in the basal layer of the epidermis, and many commercially available whitening functional ingredients have difficulty penetrating the natural barrier of the stratum corneum to reach the basal layer. This is a major reason why many whitening cosmetics are not as effective as desired.
[0003] Exosomes, with a diameter of approximately 30 nm to 200 nm, are extracellular vesicles secreted by various cell types. They possess a lipid bilayer membrane structure and contain substances such as proteins, lipids, polysaccharides, and RNA. Because their diameter is much smaller than the intercellular spaces of the stratum corneum, exosomes can directly penetrate the stratum corneum and enter the deep layers of the epidermis.
[0004] The membrane structure of exosomes is easily damaged by factors such as temperature, pH, enzymatic hydrolysis, and mechanical force, leading to leakage or inactivation of internal active ingredients. The three-dimensional network structure of the gel can form a "physical protective barrier," and the ternary nanogel can also penetrate the epidermal barrier to reach the dermis, where it superimposes the biological activity of exosomes to form a synergistic effect and enhance the whitening effect of exosomes.
[0005] Therefore, it is essential to develop a whitening skincare product that can regulate key melanin enzymes, effectively inhibit melanin production, improve pigmentation and uneven skin tone, and has good whitening and anti-aging functions. Summary of the Invention
[0006] The purpose of this invention is to provide a mixed exosome whitening gel, its preparation method, and its application, in order to solve the problems existing in the prior art.
[0007] The technical solution adopted by this invention to solve the technical problem is as follows:
[0008] The present invention provides a mixed exosome whitening gel, which, by percentage, comprises the following components: 50-60% milk exosomes, 5-10% ginseng exosomes, and 5-10% centella asiatica exosomes, loaded in a ternary composite gel network to form an intelligent responsive synergistic system, achieving intelligent and targeted release of exosomes in the dermis.
[0009] This invention provides a method for preparing a mixed exosome whitening gel, comprising the following steps:
[0010] (1) Extraction of milk exosomes;
[0011] (2) Extraction of ginseng exosomes;
[0012] (3) Extraction of Centella asiatica exosomes;
[0013] (4) The extracted milk exosomes, ginseng exosomes and centella asiatica exosomes were loaded into a ternary composite gel network to form an intelligent responsive synergistic system, thereby obtaining a mixed exosome whitening gel.
[0014] As a preferred embodiment, the method for extracting milk exosomes is as follows:
[0015] Fresh skim milk was centrifuged at 3000-5000g for 30-60 minutes at 4°C, and the supernatant was removed. The milk was then centrifuged at 10000-12000g for 20-40 minutes to remove cell debris. The supernatant was collected and filtered through a 0.22µm filter membrane. The filtrate was collected and incubated overnight at 4°C with 8-10% PEG6000. The milk was then centrifuged at 10000-12000g for 20-40 minutes, and the supernatant was removed. The precipitate was dissolved in 1-10mL of PBS buffer to obtain milk exosomes.
[0016] As a preferred embodiment, the method for extracting ginseng exosomes is as follows:
[0017] Take 500-1000g of fresh ginseng, wash it, homogenize it, and extract the juice. Centrifuge at 3000-5000g for 30-60 minutes at 4℃, remove the supernatant, centrifuge at 10000-12000g for 20-40 minutes to remove cell debris, filter the supernatant through a 0.22um filter membrane, collect the filtrate, add 8-10% PEG6000 to the filtrate and incubate overnight at 4℃, centrifuge at 10000-12000g for 40-60 minutes to remove the supernatant, dissolve the precipitate in 1-10mL PBS buffer solution to obtain ginseng exosomes.
[0018] As a preferred embodiment, the method for extracting Centella asiatica exosomes is as follows:
[0019] Take 500-1000g of fresh Centella asiatica, wash and juice it. Centrifuge at 3000-5000g for 30-60 minutes at 4℃, remove the supernatant, centrifuge at 10000-12000g for 20-40 minutes to remove cell debris, collect the supernatant, filter it through a 0.22um filter membrane, collect the filtrate, add 8-10% PEG6000 to the filtrate and incubate overnight at 4℃, centrifuge at 10000-12000g for 40-60 minutes to remove the supernatant, dissolve the precipitate in 1-10mL PBS buffer solution to obtain Centella asiatica exosomes.
[0020] As a preferred embodiment, the specific operation process of step (4) is as follows:
[0021] At a low temperature of 4℃, PNIPAM sol, HA solution and 15% GelMA solution were mixed in a volume ratio of (0.5-1):(1-4):(4-8), and gently vortexed for 10-20 minutes. The photoinitiator LAP was then added to the mixture to obtain a ternary sol.
[0022] After mixing milk exosomes, ginseng exosomes and centella asiatica exosomes evenly to form a mixed exosome weight suspension, slowly drip it into the ternary sol and continue to stir at low temperature for 6-15 minutes.
[0023] A ternary sol loaded with exosomes was injected into a mold to form a stable composite gel.
[0024] In a preferred embodiment, the final concentration of the photoinitiator LAP in the ternary sol is 0.05%-0.1% w / v.
[0025] In a preferred embodiment, the mixed exosome whitening gel contains a mixed exosome resuspension at a concentration of 8%-13% v / v.
[0026] As a preferred embodiment, the ternary sol loaded with exosomes is injected into a mold, placed in a 37°C constant temperature oven for 3-7 minutes, and then irradiated with blue light for 1-2 minutes to form a stable composite gel.
[0027] This invention provides the application of a mixed exosome whitening gel in the preparation of whitening skin care products.
[0028] The beneficial effects of this invention are:
[0029] The present invention provides a mixed exosome whitening gel, which mainly includes milk exosomes, ginseng exosomes and centella asiatica exosomes. Each exosome is loaded in a ternary composite gel network to form an intelligent responsive synergistic system, which can realize the intelligent release of exosomes in the dermis and solve the core problems of poor exosome stability and low delivery.
[0030] The present invention provides a mixed exosome whitening gel with extremely strong skin permeability: the size of the exosomes (40-300nm) is much smaller than the gap between the skin's stratum corneum (50-500nm), which can directly and efficiently penetrate the skin barrier and reach the basal layer of the epidermis to target melanocytes. This solves the industry pain point of low transdermal absorption rate and poor effect of traditional whitening ingredients (such as arbutin, vitamin C, etc.).
[0031] The present invention provides a mixed exosome whitening gel that effectively inhibits melanin production by regulating key enzymes in melanin production. Its mechanism of action is gentle and can effectively improve pigmentation and uneven skin tone, and has good whitening and anti-aging functions.
[0032] This invention provides a mixed exosome whitening gel, in which all exosomes are extracted from natural edible plants and foods such as milk, ginseng, and centella asiatica. Theoretically, the biocompatibility with the human body is better than that of chemically synthesized ingredients, with expected lower irritation and less sensitization, making it more suitable for sensitive skin and long-term use. Attached Figure Description
[0033] Figure 1 This is a particle size distribution diagram of milk exosomes extracted in Example 1.
[0034] Figure 2 This is a particle size distribution diagram of ginseng exosomes extracted in Example 2.
[0035] Figure 3 This is a particle size distribution diagram of Centella asiatica exosomes extracted in Example 3.
[0036] Figure 4 This is a comparison of the effects of the mixed exosome whitening gel prepared in this invention and arbutin on inhibiting tyrosinase activity in melanoma cells in Example 5.
[0037] Figure 5 This is a comparison of the effects of the mixed exosome whitening gel prepared in this invention and arbutin on inhibiting melanin production in melanoma cells, as shown in Example 6. Detailed Implementation
[0038] In a first aspect, the present invention provides a mixed exosome whitening gel.
[0039] The present invention provides a mixed exosome whitening gel, which, by percentage, comprises the following components: 50-60% milk exosomes, 5-10% ginseng exosomes, and 5-10% centella asiatica exosomes, loaded in a ternary composite gel network to form an intelligent responsive synergistic system, achieving intelligent and targeted release of exosomes in the dermis.
[0040] Secondly, the present invention provides a method for preparing a mixed exosome whitening gel.
[0041] The present invention provides a method for preparing a mixed exosome whitening gel, which specifically includes the following steps:
[0042] (1) Extraction of milk exosomes;
[0043] Fresh skim milk was centrifuged at 3000-5000g for 30-60 minutes at 4°C, and the supernatant was removed. The milk was then centrifuged at 10000-12000g for 20-40 minutes to remove cell debris. The supernatant was collected and filtered through a 0.22µm filter membrane. The filtrate was collected and incubated overnight at 4°C with 8-10% PEG6000. The milk was then centrifuged at 10000-12000g for 20-40 minutes, and the supernatant was removed. The precipitate was dissolved in 1-10mL of PBS buffer to obtain milk exosomes.
[0044] (2) Extraction of ginseng exosomes;
[0045] Take 500-1000g of fresh ginseng, wash it, homogenize it, and extract the juice. Centrifuge at 3000-5000g for 30-60 minutes at 4℃, remove the supernatant, centrifuge at 10000-12000g for 20-40 minutes to remove cell debris, filter the supernatant through a 0.22um filter membrane, collect the filtrate, add 8-10% PEG6000 to the filtrate and incubate overnight at 4℃, centrifuge at 10000-12000g for 40-60 minutes to remove the supernatant, dissolve the precipitate in 1-10mL PBS buffer solution to obtain ginseng exosomes.
[0046] (3) Extraction of Centella asiatica exosomes;
[0047] Take 500-1000g of fresh Centella asiatica, wash and juice it. Centrifuge at 3000-5000g for 30-60 minutes at 4℃, remove the supernatant, centrifuge at 10000-12000g for 20-40 minutes to remove cell debris, collect the supernatant, filter it through a 0.22um filter membrane, collect the filtrate, add 8-10% PEG6000 to the filtrate and incubate overnight at 4℃, centrifuge at 10000-12000g for 40-60 minutes to remove the supernatant, dissolve the precipitate in 1-10mL PBS buffer solution to obtain Centella asiatica exosomes.
[0048] (4) The extracted milk exosomes, ginseng exosomes and centella asiatica exosomes were loaded into a ternary composite gel network to form an intelligent responsive synergistic system, thereby obtaining a mixed exosome whitening gel.
[0049] Specifically, at a low temperature of 4℃, PNIPAM sol, HA solution and 15% GelMA solution were mixed in a volume ratio of (0.5-1):(1-4):(4-8), and gently vortexed for 10-20 minutes. The photoinitiator LAP was added to the resulting mixture to obtain a ternary sol. Milk exosomes, ginseng exosomes and centella asiatica exosomes were mixed evenly to form a mixed exosome weight suspension, which was slowly dripped into the ternary sol and stirred at low temperature for 6-15 minutes. The ternary sol loaded with exosomes was injected into a mold to form a stable composite gel.
[0050] Preferably, the final concentration of the photoinitiator LAP in the ternary sol is 0.05%-0.1% w / v.
[0051] Preferably, in the mixed exosome whitening gel, the proportion of mixed exosome weight suspension is 8%-13% v / v.
[0052] Preferably, the ternary sol loaded with exosomes is injected into a mold, placed in a 37°C constant temperature oven for 3-7 minutes, and then irradiated with blue light for 1-2 minutes to form a stable composite gel.
[0053] Thirdly, the present invention provides an application of a mixed exosome whitening gel in the preparation of whitening skin care products.
[0054] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.
[0055] Example 1: Method for extracting milk exosomes
[0056] Fresh skim milk was centrifuged at 3000g for 30 minutes at 4℃, and the supernatant was removed. The milk was then centrifuged at 10000g for 20 minutes to remove cell debris. The supernatant was collected and filtered through a 0.22µm filter membrane. The filtrate was collected, and 10% PEG6000 was added to the filtrate. The mixture was incubated overnight at 4℃, centrifuged at 10000g for 40 minutes, and the supernatant was removed. The precipitate was dissolved in 1mL of PBS buffer to obtain milk exosomes. The size and distribution of the exosomes were determined using an NTA (nanoscale tracking analyzer). The milk exosomes were distributed in the 40-300nm range, with the main peak at 131nm, and no other impurity peaks were observed. Figure 1 ).
[0057] Example 2: Method for extracting ginseng exosomes
[0058] Take 1000g of fresh ginseng, wash it, homogenize it, and extract the juice. Centrifuge at 3000g for 30 minutes at 4℃, remove the supernatant, centrifuge at 10000g for 20 minutes to remove cell debris, collect the supernatant, filter it through a 0.22um filter membrane, collect the filtrate, add an equal proportion of 10% PEG6000 to the filtrate and incubate overnight at 4℃, centrifuge at 10000g for 40 minutes, remove the supernatant, dissolve the precipitate in 1mL PBS buffer solution to obtain ginseng exosomes. The particle size and distribution of the exosomes were determined using NTA (Nanoscale Tracking Analyzer). The particle size distribution of the ginseng exosomes was in the range of 40-300nm, with the main peak at 165nm, and no other impurity peaks. Figure 2 ).
[0059] Example 3: Method for extracting Centella asiatica exosomes
[0060] 1000g of fresh Centella asiatica was washed, juiced, and centrifuged at 3000g for 30 minutes at 4℃. The supernatant was removed, and the mixture was centrifuged at 10000g for 20 minutes to remove cell debris. The supernatant was then filtered through a 0.22µm filter membrane. The filtrate was collected, and 10% PEG6000 was added to the filtrate. The mixture was incubated overnight at 4℃, centrifuged at 10000g for 40 minutes, and the supernatant was removed. The precipitate was dissolved in 1mL of PBS buffer to obtain Centella asiatica exosomes. The particle size and distribution of the exosomes were determined using an NTA (nanoscale tracking analyzer). The particle size distribution of the Centella asiatica exosomes was in the range of 40-300nm, with the main peak at 136nm and no other impurity peaks. Figure 3 ).
[0061] Example 4: Preparation of a mixed exosome whitening gel
[0062] At a low temperature of 4℃, PNIPAM sol, HA solution, and 15% GelMA solution were mixed at a volume ratio of 1:3:6 and gently vortexed for 15 minutes. The photoinitiator LAP was then added to the mixture to obtain a ternary sol with a final concentration of 0.05% w / v. The milk exosomes, ginseng exosomes, and centella asiatica exosomes prepared above were mixed evenly at a mass fraction of 55% milk exosomes, 8% ginseng exosomes, and 7% centella asiatica exosomes to form a mixed exosome weight suspension. This mixture was slowly dripped into the ternary sol and stirred at low temperature for 10 minutes, with the mixed exosome weight suspension accounting for 10% v / v. The ternary sol loaded with exosomes was injected into a mold, placed in a constant temperature oven at 37℃ for 5 minutes, and then irradiated with blue light for 1.5 minutes to form a stable composite gel, thus obtaining a mixed exosome whitening gel.
[0063] Example 5: Experiment on Inhibition of Tyrosinase Activity
[0064] Take 1×10 B16F10 cells in logarithmic growth phase 5 One sample was inoculated into a six-well plate. After 24 hours, the supernatant was discarded. The negative control group received complete culture medium, the positive control group received 100 nM arbutin α-MSH, and the treatment groups received α-MSH plus different concentrations of mixed exosome whitening gel (divided according to vesicle number, low concentration 1×10⁻⁶). 9 5×10⁵ cells / mL 9 Cells / mL, height 1×10 10Three dose groups (3 cells / mL) were set up, with three replicates per group. After incubation for 72 h, the culture medium was discarded, cells were collected, and 1 ml of PBS solution containing 1% Triton X-100 was placed in each well. After mixing, 100 μL was aspirated and placed in a 96-well plate, and 100 μL of PBS solution containing 2 μM levodopa was added and mixed. After incubation at 37 °C for 1 h, the absorbance was measured at 475 nm.
[0065] The results are as follows Figure 4 As shown, the number of mixed exosome vesicles is 1×10 9 The concentration of mixed exosomes / mL significantly inhibited tyrosinase activity in melanoma cells (inhibition rate 28.91±2.36%), and its inhibitory effect was comparable to that of the positive control group arbutin α-MSH (inhibition rate 23.78±1.8%); the number of mixed exosome vesicles was 5×10⁻⁶. 9 A concentration of 5 × 10⁶ cells / mL or higher can significantly inhibit tyrosinase activity (5 × 10⁶ cells / mL). 9 The inhibition rate was 52.91 ± 1.38% per 10^6 cells / mL, and 1 × 10^6 cells / mL was 10^6 cells / mL. 10 The inhibition rate (per cell / mL) was 65.68±2.14%, which was significantly better than the inhibitory effect of arbutin α-MSH in the positive control group. This indicates that the mixed exosome whitening composition prepared in this invention can inhibit the activity of tyrosinase in melanoma cells, and the effect is better than that of arbutin.
[0066] Example 6: Assay Experiment for Inhibition of Melanin Production
[0067] Take 1×10 B16F10 cells in logarithmic growth phase 5 One sample was inoculated into a six-well plate. After 24 hours, the supernatant was discarded. The negative control group received complete culture medium, the positive control group received 100 nM arbutin α-MSH, and the treatment groups received α-MSH plus different concentrations of mixed exosome whitening gel (divided according to vesicle number, low concentration 1×10⁻⁶). 9 5×10⁵ cells / mL 9 Cells / mL, height 1×10 10 Three dose groups (3 cells / mL) were set up, with three replicates per group. After incubation for 72 h, the culture medium was discarded, cells were collected, and 1 mL of 1 mol / L NaOH solution (containing 10% DMSO) was added to each well. The cells were incubated in an 80°C water bath for 1 h, and 200 μL of the solution was added to a 96-well plate. The absorbance was measured at 405 nm. The melanin content was calculated by comparing the absorbance of the sample with that of the standard.
[0068] The results are as follows Figure 5 As shown, the number of mixed exosome gel vesicles is 1×10 9The concentration-dependent inhibition (5×10⁶ cells / mL) significantly reduced melanin content in melanoma cells (inhibition rate 46.8±1.62%), and the inhibitory effect was significantly better than that of the positive control group arbutin α-MSH (inhibition rate 26.56±0.53%). 9 The inhibition rate was 58.93 ± 0.39% per 10^6 cells / mL, and 1 × 10^6 cells / mL. 10 The inhibition rate was 60.37 ± 0.77% (per cell / mL). This indicates that the mixed exosome gel prepared in this invention has a significant inhibitory effect on melanin production, and its effect is superior to that of arbutin.
[0069] This invention discloses a mixed exosome whitening gel, its preparation method, and its application. Those skilled in the art can refer to the content of this document and appropriately modify the process parameters to achieve the desired result. It should be particularly noted that all similar substitutions and modifications are obvious to those skilled in the art and are considered to be included in this invention. The product of this invention has been described through preferred embodiments, and those skilled in the art can clearly make modifications or appropriate alterations and combinations to the product described herein without departing from the content, spirit, and scope of this invention to realize and apply the technology of this invention.
Claims
1. A hybrid exosome whitening gel, characterized in that, The gel includes the following components in percentage: milk exosome 50-60%, ginseng exosome 5-10%, centella asiatica exosome 5-10%, loaded in ternary composite gel network, forming an intelligent responsive synergistic system, achieving intelligent directional release of exosomes in the dermis.
2. The method for preparing a mixed exosome whitening gel as described in claim 1, characterized in that, The method comprises the following steps: (1) extraction of milk exosomes; (2) extraction of ginseng exosomes; (3) extraction of centella asiatica exosomes; (4) loading the extracted milk exosomes, ginseng exosomes and centella asiatica exosomes into a ternary composite gel network to form an intelligent responsive synergistic system, thereby obtaining a mixed exosome whitening gel.
3. The method for preparing a mixed exosome whitening gel according to claim 2, characterized in that, The extraction method of the milk exosome is as follows: Fresh skimmed milk is centrifuged at 4℃, 3000-5000g for 30-60 minutes, the supernatant is removed, and 10000-12000g centrifugation is performed for 20-40 minutes to remove cell debris, the supernatant is taken, 0.22um filter membrane filtration is performed, the filtrate is collected, the filtrate is added with equal proportion of 8-10% PEG6000 at 4℃ overnight, 10000-12000g centrifugation is performed for 20-40 minutes to remove the supernatant, and 1-10ml PBS buffer solution is used to dissolve the precipitate, thereby obtaining milk exosomes.
4. The method of claim 2, wherein the mixed exosome whitening gel is prepared by mixing the exosome whitening gel and the whitening agent in a ratio of 1:1 to 1:
3. The extraction method of the ginseng exosome is as follows: Fresh ginseng 500-1000g is washed, homogenized and squeezed, 4℃, 3000-5000g centrifugation is performed for 30-60 minutes, the supernatant is removed, 10000-12000g centrifugation is performed for 20-40 minutes to remove cell debris, the supernatant is taken, 0.22um filter membrane filtration is performed, the filtrate is collected, the filtrate is added with equal proportion of 8-10% PEG6000 at 4℃ overnight, 10000-12000g centrifugation is performed for 40-60 minutes to remove the supernatant, and 1-10ml PBS buffer solution is used to dissolve the precipitate, thereby obtaining ginseng exosomes.
5. The method of claim 2, wherein the mixed exosome whitening gel is prepared by mixing the exosome whitening gel and the whitening agent in a ratio of 1:1 to 1:
3. The extraction method of the centella asiatica exosome is as follows: Fresh centella asiatica 500-1000g is washed, squeezed, 4℃, 3000-5000g centrifugation is performed for 30-60 minutes, the supernatant is removed, 10000-12000g centrifugation is performed for 20-40 minutes to remove cell debris, the supernatant is taken, 0.22um filter membrane filtration is performed, the filtrate is collected, the filtrate is added with equal proportion of 8-10% PEG6000 at 4℃ overnight, 10000-12000g centrifugation is performed for 40-60 minutes to remove the supernatant, and 1-10ml PBS buffer solution is used to dissolve the precipitate, thereby obtaining centella asiatica exosomes.
6. The method of claim 2, wherein the mixed exosome whitening gel is prepared by mixing the exosome whitening gel and the whitening agent in a ratio of 1:1 to 1:
3. The specific operation process of step (4) is as follows: In a low temperature environment of 4℃, PNIPAM sol, HA solution and 15% GelMA solution are mixed in a volume ratio of (0.5-1):(1-4):(4-8), gently vortexed for 10-20 minutes, and a light initiator LAP is added to the obtained mixture to obtain a ternary sol; The mixed exosome resuspension solution is formed by uniformly mixing the milk exosome, ginseng exosome and centella asiatica exosome, and then slowly dropping into the ternary sol, and continuing to stir for 6-15 minutes at low temperature; The ternary sol loaded with exosomes is injected into a mold to form a stable composite gel.
7. The method for preparing a mixed exosome whitening gel according to claim 6, characterized in that, In the ternary sol, the final concentration of the photoinitiator LAP is 0.05%-0.1% w / v.
8. The method for preparing a mixed exosome whitening gel according to claim 6, characterized in that, In the mixed exosome whitening gel, the proportion of mixed exosome weight suspension is 8%-13% v / v.
9. The method for preparing a mixed exosome whitening gel according to claim 6, characterized in that, The ternary sol loaded with exosomes was injected into a mold, placed in a 37°C constant temperature oven for 3-7 minutes, and then irradiated with blue light for 1-2 minutes to form a stable composite gel.
10. The application of the mixed exosome whitening gel as described in claim 1 in the preparation of whitening skin care products.