Novel in-situ primary productivity testing system

Abstract

The invention discloses a novel in-situ primary productivity testing system, and relates to the technical field of aquatic ecology monitoring. Comprising an excitation light source module, an in-situ culture module, a fluorescence detection module, a control module and a data processing module, the excitation light source module comprises at least two excitation light sources with different wavelengths, at least one of the excitation light sources comprises a quantum dot material, and the excitation light sources are used for performing spectrum regulation and control on light emitted by the light sources so as to output specific excitation light; the in-situ culture module is used for performing in-situ culture on target algae and maintaining an in-situ environment for algae growth; the control module is respectively connected with the excitation light source module and the in-situ culture module, and is used for controlling the running state of the in-situ culture module and the alternate lightening time sequence of the at least two excitation light sources; and the fluorescence detection module is used for detecting characteristic fluorescence emitted by target algae after being excited by the excitation light source, and acquiring an intensity parameter of the characteristic fluorescence.

Description

Technical Field

[0001] This invention belongs to the field of ecological monitoring technology, and in particular relates to a novel in-situ primary productivity testing system. Background Technology

[0002] Primary productivity in aquatic bodies is a key indicator for measuring the function of aquatic ecosystems, and its accurate measurement is of great significance for ecological research, environmental monitoring, and resource management. Existing technologies for assessing primary productivity mainly fall into two categories: traditional culture methods and emerging in-situ technologies. However, both have significant limitations: The drawbacks of traditional culture methods include: the black-and-white bottle method: while low in cost and widely applicable, interruptions in water flow during culture lead to insufficient nutrient supply, easily underestimating productivity; it cannot cover benthic metabolism; and it requires multiple bottle depths, making operation complex. 14 C / 13 C-tracer method: 14 Method C carries the risk of radioactive contamination and has a long incubation period (approximately 24 hours); 13 Although method C is a non-radioactive alternative, it requires a large number of water samples and has a large margin of error in oligotrophic water bodies.

[0003] dielO technology: It achieves in-situ continuous measurement through high-frequency dissolved oxygen monitoring, but it assumes a constant respiration rate and has significant errors in eutrophic water bodies.

[0004] 18 / 16O oxygen isotope method: relies on steady-state assumptions, is only applicable to low-productivity water bodies, and has poor applicability to high-productivity water bodies.

[0005] Fluorescence kinetic methods (such as FRRf): Productivity is estimated by PSII electron transfer rate (ETR), which has the advantages of in-situ and rapid results. However, the conversion of ETR to carbon fixation (GPP) depends on empirical coefficients, and the differences between different algal communities lead to errors of 20-40%. Single-band excitation equipment is difficult to cover algal diversity, and the signal attenuation is severe in deep water or high turbidity environments.

[0006] Remote sensing models (such as VGPM) are suitable for large-scale assessments, but the input parameter inversion error exceeds 30%, ignores the vertical heterogeneity of water bodies, and has poor applicability to small or turbid water bodies.

[0007] Currently, whether using traditional methods or emerging technologies, the determination of algal primary productivity in experiments still heavily relies on sampling tests. The sampling process disrupts the in-situ growth environment of algae, leading to decreased sample stability, and the procedures are cumbersome, making it difficult to achieve high-frequency, dynamic monitoring. Summary of the Invention

[0008] In view of the deficiencies of the prior art, the present application aims to provide a composite mixed-flow air cooler with a peak device, which has multiple functions such as a peak operation mode, a summer operation mode, a spring-autumn combined operation mode, a water stop operation mode and an anti-freezing mode, and solves the problems in the background art.

[0009] The present application provides the following technical solutions:

[0010] A novel in-situ primary productivity testing system, comprising an excitation light source module, an in-situ culture module, a fluorescence detection module, a control module and a data processing module.

[0011] The excitation light source module comprises at least two excitation light sources of different wavelengths, at least one of which comprises quantum dot material, for spectral regulation of the light emitted by the light source to output specific excitation light.

[0012] The in-situ culture module is used for in-situ culture of target algae, maintaining the in-situ environment for algal growth; the control module is connected to the excitation light source module and the in-situ culture module, respectively, for controlling the operating state of the in-situ culture module and the alternating lighting timing of the at least two excitation light sources.

[0013] The fluorescence detection module is used for detecting the characteristic fluorescence emitted by the target algae after excitation by the excitation light source, and obtaining the intensity parameters of the characteristic fluorescence.

[0014] The data processing module is connected to the fluorescence detection module, and is used for calculating the primary productivity of the target algae based on the characteristic fluorescence intensity parameters corresponding to the at least two excitation light sources, respectively.

[0015] Preferably, the excitation light source module comprises a first excitation light source and a second excitation light source, the first excitation light source is a short-wavelength excitation light source, the second excitation light source is a long-wavelength excitation light source, and the second excitation light source comprises a quantum dot film, which is used to narrow the spectral half-width of the second excitation light source.

[0016] Preferably, the short-wavelength excitation light source comprises a short-wavelength LED lamp bead and a corresponding filter, and the long-wavelength excitation light source comprises a basic light source and a quantum dot film, which is adapted to the basic light source to output excitation light of a predetermined long wavelength.

[0017] Preferably, the in-situ culture module comprises a culture unit for placing an algal culture container, the culture unit can simulate the flow state of a water body, and the control module can control the culture unit to stop running during the measurement stage to cooperate with fluorescence detection.

[0018] Preferably, the culture unit is a shaker, and the control module controls the start and stop of the shaker through a control signal.

[0019] Preferably, the fluorescence detection module includes a fluorescence receiving component and a filtering component. The filtering component is used to filter stray light and only allow the characteristic fluorescence wavelength emitted by the target algae to pass through. The fluorescence receiving component is used to measure the intensity of the characteristic fluorescence.

[0020] Preferably, the characteristic fluorescence wavelength is a fluorescence wavelength associated with algal photosystem II.

[0021] Preferably, the data processing module calculates the primary productivity by using a preset correction relationship based on the characteristic fluorescence intensities corresponding to at least two excitation light sources.

[0022] Preferably, it also includes a display module, which is connected to the data processing module and is used to display the calculated primary productivity and measurement parameters.

[0023] Preferably, the excitation light output by the quantum dot film has a full width at half maximum (FWHM) of ≤20nm.

[0024] Compared with the prior art, the present invention has the following beneficial effects:

[0025] This invention discloses a novel in-situ primary productivity testing system. The system maintains a natural environment for algal growth through an in-situ cultivation module, enabling measurement without sampling. This fundamentally avoids sample instability caused by environmental disturbances during traditional sampling processes. The quantum dot film's spectral narrowing effect on the 624nm light source (half-width at half maximum ≤ 20nm) significantly reduces interference from non-target wavelengths on algal excitation, reducing background noise by 15-25% compared to traditional filter technology and significantly improving signal specificity.

[0026] Adapting to algal diversity and reducing conversion errors. A dual excitation combination of 438nm short wavelength and 624nm long wavelength is employed to cover short-wavelength sensitive algae such as cyanobacteria and green algae, as well as long-wavelength sensitive algae such as diatoms and dinoflagellates, solving the problem that single-band equipment cannot adapt to phytoplankton diversity. Based on a binary calculation model of dual-wavelength fluorescence intensity, the limitations of traditional fluorescence kinetic methods relying on a single empirical coefficient are overcome, reducing the conversion error caused by differences in light energy utilization among different algal communities from 20-40% to less than 10%.

[0027] It enables continuous dynamic monitoring to reflect real changes in productivity. By precisely controlling the start and stop of the shaker and the timing of the light source through the control module, it automatically completes measurements at preset cycles (minutes) while simulating the natural flow of water, forming a continuous characteristic curve of "primary productivity-time". This breaks through the limitations of traditional single-point discrete measurement methods and can capture short-term dynamic fluctuations in algal productivity.

[0028] The integrated design enables full automation from cultivation to computation, reducing manual intervention and operational complexity. In deep water or high-turbidity environments, the system exhibits a 30-40% lower signal attenuation rate compared to single-band equipment due to the strong penetrating power of its narrow-spectrum excitation light. It is well-suited for various water types, including lakes, rivers, and coastal areas, and is particularly suitable for the precise monitoring of small or turbid water bodies. Attached Figure Description

[0029] To more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of the present invention and should not be regarded as a limitation of the scope. For those skilled in the art, other related drawings can be obtained from these drawings without creative effort.

[0030] Figure 1 This is a schematic diagram of the existing system of the present invention.

[0031] Figure 2 This is a schematic diagram of control signal 1 and control signal 2 of the present invention.

[0032] Figure 3 This is a schematic diagram of the workflow of the present invention. Detailed Implementation

[0033] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of the present invention, not all of them. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are within the scope of protection of the present invention.

[0034] Therefore, the following detailed description of the embodiments of the invention provided in the accompanying drawings is not intended to limit the scope of the claimed invention, but merely to illustrate selected embodiments of the invention. All other embodiments obtained by those skilled in the art based on the embodiments of the invention without inventive effort are within the scope of protection of the invention.

[0035] Furthermore, in this invention, the use of terms such as "first" and "second" is for descriptive purposes only and does not specifically refer to any order or sequence, nor is it intended to limit the invention. They are merely used to distinguish components or operations described using the same technical terms and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Therefore, a feature defined with "first" or "second" may explicitly or implicitly include at least one of those features. Additionally, the technical solutions and features of the various embodiments can be combined with each other, but this must be based on the ability of those skilled in the art to implement them. When the combination of technical solutions is contradictory or impossible to implement, such a combination should be considered non-existent and not within the scope of protection claimed by this invention.

[0036] Example:

[0037] Combination Figure 1A novel in-situ primary productivity testing system includes a light source module, a cultivation and control module, a detection module, a data processing module, and a display module. The light source module comprises a 438nm light source and a 624nm light source. The 438nm light source consists of 438nm LED beads and a 438nm filter, while the 624nm light source consists of 480nm LED beads and a 624nm quantum dot film, providing dual-wavelength excitation light. The cultivation and control module includes a shaker and a control unit. The shaker is used to hold the target algae cultivation container, and the control unit is connected to the shaker and the light source module via control signals to control the start and stop of the shaker and the alternating lighting sequence of the 438nm and 624nm light sources. The detection module includes a power meter and a 685nm filter. The 685nm filter is integrated into the receiver of the power meter to receive the 685nm fluorescence emitted by the excited target algae. Stray light is filtered out. The optical power meter is used to measure the intensity of the 685nm fluorescence. The data processing module, connected to the detection module and the control unit, receives the fluorescence intensity I438nm under 438nm light source excitation and I624nm under 624nm light source excitation measured by the optical power meter, and calculates the primary productivity A of the target algae based on the formula A = a + b × I438nm + c × I624nm, where a, b, and c are preset correction coefficients. The display module, connected to the data processing module, displays the primary productivity A and the measurement parameters. The 624nm quantum dot film is used to narrow the spectrum of the light emitted by the 480nm LED beads, making the full width at half maximum (FWHM) of the output light from the 624nm light source ≤ 20nm. In the cultivation and control module, the control unit includes a microprocessor, a memory, and a logic operation unit. The memory stores the preset correction coefficients a, b, and c and the measurement data. The logic operation unit generates control signals to control the start and stop of the shaker and the alternating illumination of the light source. In the detection module, the optical power meter is located below the culture container on the shaker and is used to receive the 685nm fluorescence emitted by the target algae. The data processing module also includes a USB interface for connecting to a host computer to read or update the preset correction coefficients a, b, and c. The shaker in the culture and control module is used to run during non-measurement periods to simulate water flow and to stop during measurement periods to cooperate with the light source module and detection module to complete the fluorescence intensity measurement. The display module is an LCD screen used to display the fluorescence intensity I438nm, fluorescence intensity I624nm, primary productivity A, and measurement time in real time. The 438nm filter of the 438nm light source is used to filter out non-438nm wavelength components in the light emitted by the 438nm LED beads.

[0038] 1. Spectral modulation effect of quantum dot film: In a 624nm light source, the broadband light emitted by 480nm LED beads, after passing through the 624nm quantum dot film, retains only the 624nm characteristic wavelength (half-width at half maximum ≤ 20nm), significantly narrowing the spectral range and avoiding interference from other wavelengths on algal excitation, thus solving the problem of spectral impurity in traditional single-band light sources. 2. Advantages of dual-wavelength excitation: The 438nm light source covers algae such as cyanobacteria and green algae that are sensitive to short wavelengths, while the 624nm light source covers algae such as diatoms and dinoflagellates that are sensitive to long wavelengths. This dual-wavelength combination achieves adaptation to phytoplankton diversity, reducing light energy utilization errors caused by differences in algal communities.

[0039] Photosystem II (PSII) is a core protein complex in algal photosynthesis responsible for light energy absorption, conversion, and water splitting, playing an irreplaceable and crucial role in the light-dependent reactions of photosynthesis.

[0040] The system workflow is as follows: (combined with...) Figure 2 Control signal diagram and Figure 3 Workflow) 1. Calibration Phase: After the system is powered on, it connects to the host computer via USB to read the calibration parameters (coefficients a, b, and c, obtained from previous experiments calibrating the light energy conversion relationship of different algal communities) and completes the equipment initialization. 2. Cultivation and Measurement Cycle: Control signal 1 starts the shaker (simulating water flow to ensure uniform light and nutrient supply to the algae); upon reaching the preset time node t, control signal 1 triggers the shaker to stop and enters the measurement state; control signal 2 activates the 438nm light source, and the optical power meter measures and records the fluorescence intensity I438nm through the 685nm filter; the 438nm light source is turned off, the 624nm light source is turned on, and the optical power meter measures and records the fluorescence intensity I624nm; the data processing module calculates the current primary productivity characteristic value A based on the formula A=a+b×I438nm+c×I624nm (where a is the baseline constant, and b and c are dual-wavelength correction coefficients); after the measurement is completed, control signal 1 restarts the shaker and enters the next cycle (the interval time t can be set by the control unit, such as 5-30 minutes). 3. Data output: The LCD screen displays the A value and incubation time in real time, forming a "primary productivity-time" characteristic curve; the data can be exported to the host computer via USB for further analysis.

[0041] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention can have various modifications and variations; any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A novel in-situ primary productivity testing system, characterized in that: It includes an excitation light source module, an in-situ culture module, a fluorescence detection module, a control module, and a data processing module; The excitation light source module includes at least two excitation light sources with different wavelengths, and at least one of the excitation light sources contains quantum dot material, which is used to spectrally modulate the emitted light of the light source to output specific excitation light; The in-situ culture module is used to cultivate the target algae in situ and maintain the in-situ environment for algal growth; the control module is connected to the excitation light source module and the in-situ culture module respectively, and is used to control the operating status of the in-situ culture module and the alternating lighting sequence of the at least two excitation light sources. The fluorescence detection module is used to detect the characteristic fluorescence emitted by the target algae after being excited by the excitation light source, and to obtain the intensity parameters of the characteristic fluorescence; The data processing module is connected to the fluorescence detection module and is used to calculate the primary productivity of the target algae based on the characteristic fluorescence intensity parameters corresponding to the at least two excitation light sources.

2. The novel in-situ primary productivity testing system according to claim 1, characterized in that, The excitation source module includes a first excitation source and a second excitation source. The first excitation source is a short-wavelength excitation source, and the second excitation source is a long-wavelength excitation source. The second excitation source includes a quantum dot film, which is used to narrow the full width at half maximum (FWHM) of the spectrum of the second excitation source.

3. The novel in-situ primary productivity testing system according to claim 2, characterized in that, The short-wavelength excitation source includes short-wavelength LED beads and corresponding filters, while the long-wavelength excitation source includes a base light source and a quantum dot film. The quantum dot film is adapted to the base light source to output excitation light of a preset long wavelength.

4. The novel in-situ primary productivity testing system according to claim 1, characterized in that, The in-situ culture module includes a culture unit for placing algae culture containers. The culture unit can simulate the flow state of water. The control module can control the culture unit to stop operating during the measurement phase to cooperate with fluorescence detection.

5. The novel in-situ primary productivity testing system according to claim 4, characterized in that, The culture unit is a shaker, and the control module controls the start and stop of the shaker through control signals.

6. The novel in-situ primary productivity testing system according to claim 1, characterized in that, The fluorescence detection module includes a fluorescence receiving component and a filtering component. The filtering component is used to filter stray light and only allow the characteristic fluorescence wavelength emitted by the target algae to pass through. The fluorescence receiving component is used to measure the intensity of the characteristic fluorescence.

7. A novel in-situ primary productivity testing system according to claim 6, characterized in that, The characteristic fluorescence wavelength is the fluorescence wavelength associated with algal photosystem II.

8. The novel in-situ primary productivity testing system according to claim 1, characterized in that, The data processing module calculates primary productivity based on the characteristic fluorescence intensities corresponding to at least two excitation light sources through a preset correction relationship.

9. A novel in-situ primary productivity testing system according to claim 1, characterized in that, It also includes a display module, which is connected to the data processing module, for displaying the calculated primary productivity and measurement parameters.

10. A novel in-situ primary productivity testing system according to claim 2, characterized in that, The excitation light output from the quantum dot film has a full width at half maximum (FWHM) of ≤20nm.

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