Microbial activator based on compound nutritional factors and preparation method thereof

Abstract

The invention discloses a microbial activator based on compound nutritional factors and a preparation method thereof, and belongs to the technical field of microbial activators. The amino acid chelated trace elements are prepared by chelating Fe, Zn and Mn metal salts with a glutamic acid / aspartic acid solution; mixing with glucose, fructose, ATP-gamma-S and coenzyme Q10, emulsifying, embedding with trehalose, and freeze-drying to form quick-acting microcapsules; mixing and granulating gelatinized corn starch, yeast peptone, calcium and magnesium phytate and PHA (polyhydroxyalkanoate) to prepare sustained-release pellets; and finally, coating the pellets with an HPMC aqueous solution and an ethyl cellulose ethanol solution by using a fluidized bed to form the difunctional activator particles with a quick release layer and a slow release layer. A three-stage time sequence release structure is designed, the three defects of unstable quick-acting components, asynchronous nutrition release and trace element passivation of a traditional activator are synchronously overcome, and the colonization efficiency of a microbial fertilizer and a microbial agent is remarkably improved.

Description

Technical Field

[0001] This invention belongs to the field of microbial activator technology, specifically relating to a microbial activator based on compound nutrient factors and its preparation method. Background Technology

[0002] With the widespread application of microorganisms in green agriculture, microbial activators, as core synergistic components, directly influence the colonization and functional expression of soil microorganisms through their nutrient supply efficiency. In the field of sustainable agricultural development, microbial fertilizers and straw-degrading inoculants, as core green production technologies, rely on the efficient colonization and metabolic activity of functional microorganisms for their effectiveness. Microbial activators, as key synergistic carriers, directly determine the activation speed and functional sustainability of microbial communities by providing carbon sources, nitrogen, phosphorus, and trace elements. However, existing activators suffer from three major drawbacks: unstable fast-acting components, asynchronous nutrient release, and trace element deactivation, leading to low colonization efficiency of functional bacteria in microbial fertilizers. Developing novel activators that combine precise timing of release with efficient element utilization has become a key requirement for overcoming the bottlenecks in agricultural microbial applications. Summary of the Invention

[0003] In view of this, the purpose of this invention is to provide a microbial activator based on compound nutrient factors and its preparation method. This invention simultaneously solves the three major defects of traditional activators: unstable fast-acting components, asynchronous nutrient release, and passivation of trace elements, and significantly improves the colonization efficiency of microbial fertilizers and inoculants.

[0004] To achieve the above objectives, the present invention provides the following technical solution:

[0005] This invention provides a method for preparing a microbial activator based on composite nutrient factors, comprising the following steps:

[0006] S1. The metal salt solution was slowly added dropwise to the amino acid solution, and the acetate buffer was added to maintain the pH at 5.5-5.8. Then the reaction was carried out at a constant temperature with stirring. After the reaction was completed, the amino acid chelated trace elements were obtained by spray drying.

[0007] S2. The amino acid chelated trace elements, glucose, and fructose are mixed in water at 60-65°C, and then cooled to 40-45°C. ATP-γ-S and coenzyme Q10 are added and stirred evenly. The mixture is then emulsified and freeze-granulated. The granules are then immersed in trehalose solution, sonicated, filtered, and freeze-dried to obtain fast-acting microcapsules.

[0008] S3. Mix gelatinized corn starch, yeast peptone, calcium magnesium phytate, and PHA powder evenly and granulate to obtain sustained-release microspheres;

[0009] S4. Add the sustained-release microspheres and the fast-acting microcapsules to a fluidized bed, then start fluidization, using a 3% HPMC aqueous solution as the spray liquid, a spraying rate of 8 g / min, and an atomization pressure of 0.3 MPa. When the coating weight is 5 wt%, switch the spray liquid to a 5% ethyl cellulose ethanol solution. When the coating weight is 8 wt%, stop the process, remove the particles, and dry them to obtain the microbial activator.

[0010] Preferably, the metal salt solution in S1 is a blend of FeSO4, ZnSO4, and MnCl2, and the mass ratio of FeSO4, ZnSO4, MnCl2, and water is (30-40):(5-10):1.5:500.

[0011] Preferably, the amino acid solution in S1 is a blend of glutamic acid and aspartic acid, and the mass ratio of glutamic acid, aspartic acid and water is 100:(60-80):200.

[0012] In some embodiments of the present invention, the temperature of the isothermal stirring reaction in S1 is 60°C, the reaction time is 35 min, and the stirring rate is 200 rpm.

[0013] Preferably, the particle size of the amino acid chelated trace elements in S1 is 50-100 μm.

[0014] Preferably, the mass ratio of amino acid chelated trace elements, glucose, fructose, water, ATP-γ-S, and coenzyme Q10 in S2 is 120:(30-50):(10-30):500:(1-5):1.5.

[0015] In some embodiments of the present invention, the concentration of the trehalose solution in S2 is 12-15 wt%.

[0016] In some embodiments of the present invention, the ultrasonic power in S2 is 200W.

[0017] Preferably, the microcapsule size of the fast-acting layer in S2 is 450-800 μm.

[0018] In some embodiments of the present invention, the method for preparing the gelatinized corn starch in S3 is as follows: 180g of corn starch is added to 1L of boiling water, gelatinized at 90°C for 20min to a semi-transparent colloid, and then cooled to 50°C and kept warm.

[0019] Preferably, the mass ratio of gelatinized corn starch, yeast peptone, calcium magnesium phytate, and PHA powder in S3 is 100:40:25:35.

[0020] Preferably, the particle size of the sustained-release microspheres in S3 is 1-1.2 mm.

[0021] Preferably, the mass ratio of the sustained-release microspheres to the fast-acting microcapsules in S4 is 2:1.

[0022] In some embodiments of the present invention, the HPMC aqueous solution in S4 further includes 2% wt% nano-SiO2.

[0023] This invention provides a microbial activator based on composite nutrient factors, which is prepared by the above-described preparation method.

[0024] It contains at least the following beneficial technical effects:

[0025] This invention stabilizes trace elements (Fe, Zn, Mn) through glutamic acid / aspartic acid double chelation technology, improving the retention rate of available iron in alkaline soils. It also activates microbial nitrogen fixation and stress resistance functions using a specific ratio (Fe:Zn:Mn = 35:8:1.5). A three-stage time-release structure is designed: a trehalose fast-acting layer provides glucose, fructose, and the metabolic activator ATP-γ-S; an HPMC / ethyl cellulose coating layer controls medium-speed release; and a PHA slow-release core maintains long-term nutrient supply. This simultaneously addresses the three major defects of traditional activators: unstable fast-acting components, asynchronous nutrient release, and trace element passivation, significantly improving the colonization efficiency of microbial fertilizers and inoculants. Detailed Implementation

[0026] This invention provides a method for preparing a microbial activator based on composite nutrient factors, comprising the following steps:

[0027] S1. The metal salt solution was slowly added dropwise to the amino acid solution, and the acetate buffer was added to maintain the pH at 5.5-5.8. Then the reaction was carried out at a constant temperature with stirring. After the reaction was completed, the amino acid chelated trace elements were obtained by spray drying.

[0028] S2. The amino acid chelated trace elements, glucose, and fructose are mixed in water at 60-65°C, and then cooled to 40-45°C. ATP-γ-S and coenzyme Q10 are added and stirred evenly. The mixture is then emulsified and freeze-granulated. The granules are then immersed in trehalose solution, sonicated, filtered, and freeze-dried to obtain fast-acting microcapsules.

[0029] S3. Mix gelatinized corn starch, yeast peptone, calcium magnesium phytate, and PHA powder evenly and granulate to obtain sustained-release microspheres;

[0030] S4. Add the sustained-release microspheres and the fast-acting microcapsules to a fluidized bed, then start fluidization, using a 3% HPMC aqueous solution as the spray liquid, a spraying rate of 8 g / min, and an atomization pressure of 0.3 MPa. When the coating weight is 5 wt%, switch the spray liquid to a 5% ethyl cellulose ethanol solution. When the coating weight is 8 wt%, stop the process, remove the particles, and dry them to obtain the microbial activator.

[0031] This invention involves slowly adding a metal salt solution dropwise to an amino acid solution, then adding an acetate buffer to maintain the pH at 5.5–5.8, followed by a constant-temperature stirring reaction. After the reaction is complete, the mixture is spray-dried to obtain amino acid-chelated trace elements. By employing the above technical solution, this invention coordinates the carboxyl group of glutamic acid / aspartic acid with the amino group of a bidentate metal ion to form a stable five-membered ring chelate, blocking the binding of the ion to soil hydroxyl groups. This results in an increase in the effective iron retention rate to 85% in alkaline soil (pH 7.9) and an increase in intracellular iron enrichment in microbial cells.

[0032] Preferably, the metal salt solution in S1 is a blend of FeSO4, ZnSO4, and MnCl2, wherein the mass ratio of FeSO4, ZnSO4, MnCl2, and water is (30-40):(5-10):1.5:500; the mass ratio is further preferably 35:8:1.5:500. This invention uses this ratio to make Zn... 2+ Activation of ALP releases phosphate groups for Fe-S protein synthesis; Mn-SOD and Fe-SOD synergistically reduce cellular oxidative damage; Mn 2+ Initiation of lignin depolymerization Fe 2+ Catalyzing the opening of aromatic rings drives microorganisms to complete core functions such as nitrogen fixation and straw degradation.

[0033] Preferably, the amino acid solution in S1 is a blend of glutamic acid and aspartic acid, wherein the mass ratio of glutamic acid, aspartic acid, and water is 100:(60-80):200; the mass ratio is further preferably 100:70:200. The glutamic acid + aspartic acid double chelate system utilizes long-chain glutamic acid to stabilize large ionic radius Fe. 2+ Precise coordination of short-chain aspartic acid with Zn 2+ / Mn 2 + Glutamate drives energy metabolism, while aspartic acid promotes enzyme synthesis, covering the entire growth cycle of microorganisms. This overcomes the limitations of traditional single chelating agents (such as EDTA) in terms of biotoxicity and limited functionality, providing microorganisms with a smart carrier that combines nutrient supply and metabolic activation.

[0034] In some embodiments of the present invention, the temperature of the isothermal stirring reaction in S1 is 60°C, the reaction time is 35 min, and the stirring rate is 200 rpm.

[0035] Preferably, the particle size of the amino acid chelated trace elements in S1 is 50-100 μm, and more preferably 80 μm.

[0036] In this invention, the amino acid chelated trace elements, glucose, and fructose are mixed in water at 60-65°C, and then cooled to 40-45°C. ATP-γ-S and coenzyme Q10 are added, stirred evenly, emulsified, and then freeze-granulated. The mixture is then immersed in trehalose solution, sonicated, filtered, and freeze-dried to obtain fast-acting microcapsules.

[0037] Preferably, the mass ratio of amino acid chelated trace elements, glucose, fructose, water, ATP-γ-S, and coenzyme Q10 in S2 is 120:(30-50):(10-30):500:(1-5):1.5; the mass ratio is further preferably 120:40:20:500::3:1.5.

[0038] By employing the above-mentioned scheme, the phosphate-thiosulfate bond (PS bond) of ATP-γ-S can resist hydrolysis by soil phosphatase, extending its half-life to 30 hours. As an ATP analog, it binds to the purine receptor (PurR) of microorganisms, activates the intracellular kinase cascade reaction, and directly participates in the phosphorylation reaction, providing initial energy for microbial proliferation. Coenzyme Q10 can enhance microbial respiration and increase microbial energy production.

[0039] In some embodiments of the present invention, the concentration of the trehalose solution in S2 is 12-15 wt%.

[0040] In some embodiments of the present invention, the ultrasonic power in S2 is 200W.

[0041] Preferably, the microcapsule size of the fast-acting layer in S2 is 450-800 μm, and more preferably 600 μm.

[0042] This invention involves uniformly mixing gelatinized corn starch, yeast peptone, calcium magnesium phytate, and PHA powder, and then granulating the mixture to obtain sustained-release microspheres.

[0043] In some embodiments of the present invention, the method for preparing the gelatinized corn starch in S3 is as follows: 180g of corn starch is added to 1L of boiling water, gelatinized at 90°C for 20min to a semi-transparent colloid, and then cooled to 50°C and kept warm.

[0044] Preferably, the mass ratio of gelatinized corn starch, yeast peptone, calcium magnesium phytate, and PHA powder in S3 is 100:40:25:35.

[0045] Preferably, the particle size of the sustained-release microspheres in S3 is 1-1.2 mm, and more preferably 1 mm.

[0046] In this invention, sustained-release microspheres and fast-acting microcapsules are fed into a fluidized bed, and then fluidization is started. A 3% HPMC aqueous solution is used as the spray liquid, the spraying rate is 8 g / min, and the atomization pressure is 0.3 MPa. When the coating weight is 5 wt%, the spray liquid is switched to a 5% ethyl cellulose ethanol solution. When the coating weight is 8 wt%, the particles are removed and dried to obtain a microbial activator.

[0047] Preferably, the mass ratio of the sustained-release microspheres to the fast-acting microcapsules in S4 is 2:1.

[0048] The HPMC aqueous solution in S4 also contains 2% wt% SiO2.

[0049] This invention provides a microbial activator based on composite nutrient factors, which is prepared by the above-described preparation method.

[0050] This invention sets the microbial activator as a multi-layer structure. After application, the trehalose shell on the surface of the fast-acting layer dissolves within 30 seconds upon contact with water, rapidly releasing a fast-acting carbon source, amino acid chelated trace elements, and microbial promoters (ATP-γ-S, coenzyme Q10), quickly activating microbial activity. After the HPMC layer is completely dissolved, the slow release of nitrogen and carbon sources is initiated, and then diffuses through the EC membrane. The PHA chain is subsequently degraded by microorganisms to maintain long-term nutrient release. This is achieved through a three-level structural design of a trehalose fast-release layer (time-controlled), an HPMC / EC controlled-release membrane (rate-controlled), and a starch-PHA slow-release core (quantity-controlled).

[0051] Based on the embodiments of this invention, all other embodiments obtained by those skilled in the art without inventive effort are within the scope of protection of this invention. The invention will now be described with reference to specific embodiments. It should be noted that these embodiments are merely descriptive and do not limit the invention in any way.

[0052] Example 1

[0053] This embodiment provides a method for preparing a microbial activator based on composite nutrient factors, the preparation steps of which are as follows:

[0054] S1. Preparation of metal salt solution: Mix FeSO4, ZnSO4, MnCl2 and water in a mass ratio of 35:8:1.5:500 until homogeneous;

[0055] Preparation of amino acid solution: Mix glutamic acid, aspartic acid and water in a mass ratio of 100:70:200 until homogeneous;

[0056] The metal salt solution was slowly added dropwise to the amino acid solution, and the pH was maintained at 5.6 with acetate buffer. The reaction was then carried out at 60℃ and 200rpm for 35 minutes with constant temperature stirring. After the reaction was completed, the amino acid chelated trace elements were obtained by spray drying.

[0057] S2. The amino acid chelated trace elements, glucose, and fructose were mixed in water at 63°C, then cooled to 43°C. ATP-γ-S and coenzyme Q10 were added, stirred until homogeneous, emulsified, and then freeze-granulated. The mixture was then immersed in a 13wt% trehalose solution at 200W for ultrasonication, filtration, and freeze-drying to obtain fast-acting microcapsules with a particle size of 600μm. The mass ratio of amino acid chelated trace elements, glucose, fructose, water, ATP-γ-S, and coenzyme Q10 was 120:40:20:500:3:1.5.

[0058] S3. Preparation of gelatinized corn starch: Add 180g of corn starch to 1L of boiling water, gelatinize at 90℃ for 20min until a semi-transparent colloid is formed, and then cool to 50℃ and keep warm.

[0059] Gelatinized corn starch, yeast peptone, calcium magnesium phytate, and PHA powder were mixed evenly in a mass ratio of 100:40:25:35 and granulated to obtain sustained-release microspheres with a particle size of 1 mm.

[0060] S4. Add the sustained-release microspheres and the fast-acting microcapsules to a fluidized bed at a mass ratio of 2:1, then start the fluidization process. Use a 3% HPMC aqueous solution (containing 2% wt% nano-SiO2) as the spray liquid, spraying at a rate of 8 g / min and an atomization pressure of 0.3 MPa. When the coating weight reaches 5 wt%, switch the spray liquid to a 5% ethyl cellulose ethanol solution. When the coating weight reaches 8 wt%, stop the process, remove the particles, and dry them to obtain the microbial activator.

[0061] Example 2

[0062] This embodiment provides a method for preparing a microbial activator based on composite nutrient factors, the preparation steps of which are as follows:

[0063] S1. Preparation of metal salt solution: Mix FeSO4, ZnSO4, MnCl2 and water in a mass ratio of 30:5:1.5:500 until homogeneous;

[0064] Preparation of amino acid solution: Mix glutamic acid, aspartic acid and water in a mass ratio of 100:60:200 until homogeneous;

[0065] The metal salt solution was slowly added dropwise to the amino acid solution, and the acetate buffer was added to maintain the pH at 5.5-5.8. Then, the reaction was carried out at 60℃ and 200rpm for 35 minutes with constant temperature stirring. After the reaction was completed, the amino acid chelated trace elements were obtained by spray drying.

[0066] S2. The amino acid chelated trace elements, glucose, and fructose were mixed in water at 60°C, then cooled to 40°C. ATP-γ-S and coenzyme Q10 were added, stirred until homogeneous, emulsified, and then freeze-granulated. The mixture was then immersed in a 12wt% trehalose solution at 200W for ultrasonication, filtration, and freeze-drying to obtain fast-acting microcapsules with a particle size of 450μm. The mass ratio of amino acid chelated trace elements, glucose, fructose, water, ATP-γ-S, and coenzyme Q10 was 120:30:10:500:1:1.5.

[0067] S3. Preparation of gelatinized corn starch: Add 180g of corn starch to 1L of boiling water, gelatinize at 90℃ for 20min until a semi-transparent colloid is formed, and then cool to 50℃ and keep warm.

[0068] Gelatinized corn starch, yeast peptone, calcium magnesium phytate, and PHA powder were mixed evenly in a mass ratio of 100:40:25:35 and granulated to obtain sustained-release microspheres with a particle size of 1.2 mm.

[0069] S4. Add the sustained-release microspheres and the fast-acting microcapsules to a fluidized bed at a mass ratio of 2:1, then start the fluidization process. Use a 3% HPMC aqueous solution (containing 2% wt% nano-SiO2) as the spray liquid, spraying at a rate of 8 g / min and an atomization pressure of 0.3 MPa. When the coating weight reaches 5 wt%, switch the spray liquid to a 5% ethyl cellulose ethanol solution. When the coating weight reaches 8 wt%, stop the process, remove the particles, and dry them to obtain the microbial activator.

[0070] Example 3

[0071] This embodiment provides a method for preparing a microbial activator based on composite nutrient factors, the preparation steps of which are as follows:

[0072] S1. Preparation of metal salt solution: Mix FeSO4, ZnSO4, MnCl2 and water in a mass ratio of 40:10:1.5:500 until homogeneous;

[0073] Preparation of amino acid solution: Mix glutamic acid, aspartic acid and water in a mass ratio of 100:80:200 until homogeneous;

[0074] The metal salt solution was slowly added dropwise to the amino acid solution, and the acetate buffer was added to maintain the pH at 5.5-5.8. Then, the reaction was carried out at 60℃ and 200rpm for 35 minutes with constant temperature stirring. After the reaction was completed, the amino acid chelated trace elements were obtained by spray drying.

[0075] S2. The amino acid chelated trace elements, glucose, and fructose are mixed in water at 65°C, then cooled to 45°C. ATP-γ-S and coenzyme Q10 are added, stirred until homogeneous, emulsified, and then freeze-granulated. The mixture is then immersed in a 15wt% trehalose solution at 200W for ultrasonication, filtration, and freeze-drying to obtain fast-acting microcapsules with a particle size of 450-800 μm. The mass ratio of amino acid chelated trace elements, glucose, fructose, water, ATP-γ-S, and coenzyme Q10 is 120:50:30:500:5:1.5.

[0076] S3. Preparation of gelatinized corn starch: Add 180g of corn starch to 1L of boiling water, gelatinize at 90℃ for 20min until a semi-transparent colloid is formed, and then cool to 50℃ and keep warm.

[0077] Gelatinized corn starch, yeast peptone, calcium magnesium phytate, and PHA powder were mixed evenly in a mass ratio of 100:40:25:35 and granulated to obtain sustained-release microspheres with a particle size of 1.1 mm.

[0078] S4. Add the sustained-release microspheres and the fast-acting microcapsules to a fluidized bed at a mass ratio of 2:1, then start the fluidization process. Use a 3% HPMC aqueous solution (containing 2% wt% nano-SiO2) as the spray liquid, spraying at a rate of 8 g / min and an atomization pressure of 0.3 MPa. When the coating weight reaches 5 wt%, switch the spray liquid to a 5% ethyl cellulose ethanol solution. When the coating weight reaches 8 wt%, stop the process, remove the particles, and dry them to obtain the microbial activator.

[0079] Comparative Example 1

[0080] The preparation method of this comparative example is the same as that of Example 1, except that no amino acid solution is added in S1.

[0081] Comparative Example 2

[0082] The preparation method of this comparative example is the same as that of Example 1, except that ATP-γ-S is not added in S2.

[0083] Comparative Example 3

[0084] The preparation method of this comparative example is the same as that of Example 1, except that PHA powder is not added in S3.

[0085] Comparative Example 4

[0086] The preparation method of this comparative example is the same as that of Example 1, except that in S4, the sustained-release microspheres and the fast-acting microcapsules are mixed at a mass ratio of 2:1 without fluidized bed coating.

[0087] Experimental Example 1

[0088] Rapid efficacy verification experiment

[0089] Bacterial strain: Bacillus subtilis ATCC 6051, initial concentration 1×10⁻⁶ 6 CFU / mL.

[0090] Test system: 0.01M CaCl2 solution (pH 6.8) + 0.1% activator.

[0091] Testing indicators:

[0092] ATP storage capacity: luciferase method (Promega) Kit), samples are taken every 15 minutes.

[0093] Dehydrogenase activity: TTC reduction method (OD) 485 nm, in units of ΔOD / min).

[0094] Gene expression: qPCR was used to detect the expression level of the nitrogenase gene (nifH).

[0095] The test results are shown in Table 1.

[0096] Table 1

[0097]

[0098] Example 1 showed that the ATP concentration reached 38.5 μM within 30 min (compared to only 12.3 μM in Comparative Example 1), confirming that glutamate chelation + ATP-γ-S synergistically enhances rapid release; Comparative Example 2 (without ATP-γ-S) showed a 70% decrease in dehydrogenase activity, demonstrating that metabolic activation signals are irreplaceable; Comparative Example 4 (without coating) showed a 30% decrease in nifH expression due to burst release, highlighting the necessity of coating for controlled release.

[0099] Experimental Example 2

[0100] Long-lasting effect verification experiment

[0101] Add 0.1% activator to 10g of alkaline clay (pH 7.9, organic matter 1.2%) and inoculate with Bacillus subtilis (10g). 6 The number of live bacteria was measured at CFU / g (soil) and then tested at 7 and 14 days, as shown in Table 2.

[0102] Table 2

[0103] time Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 7-day viable bacterial count 28.3±1.5 8.7±0.9 15.0±1.2 10.5±0.8 20.0±1.3 14-day viable bacterial count 32.1±1.8 14±0.4 13±0.5 8.5±0.4 5.0±1.0

[0104] Example 1: Viable bacterial count maintained at 32.1 × 10⁻⁶ for 14 days. 8 CFU / g, in Comparative Example 1, due to the rapid loss of trace elements, eventually decreased to 14 ± 0.4 × 10⁻⁶. 8Comparative Example 2 showed slow bacterial growth due to lack of initial rapid activation; Comparative Example 3 showed a decrease in viable bacterial count due to carbon source shortage and insufficient long-term effectiveness; Comparative Example 4 (without coating) showed rapid initial bacterial growth due to asynchronous nutrition, followed by a decrease in bacterial count due to subsequent lack of nutrition.

[0105] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A method for preparing a microbial activator based on compound nutrient factors, characterized in that, Includes the following steps: S1. The metal salt solution was slowly added dropwise to the amino acid solution, and the acetate buffer was added to maintain the pH at 5.5-5.

8. Then the reaction was carried out at a constant temperature with stirring. After the reaction was completed, the amino acid chelated trace elements were obtained by spray drying. S2. The amino acid chelated trace elements, glucose, and fructose are mixed in water at 60-65°C, and then cooled to 40-45°C. ATP-γ-S and coenzyme Q10 are added, stirred evenly, emulsified, and then frozen granulated. The mixture is then immersed in trehalose solution for ultrasonication, filtration, and freeze-drying to obtain fast-acting microcapsules. S3. Mix gelatinized corn starch, yeast peptone, calcium magnesium phytate, and PHA powder evenly and granulate to obtain sustained-release microspheres. S4. Add the sustained-release microspheres and the fast-acting microcapsules to a fluidized bed, then start fluidization, using a 3% HPMC aqueous solution as the spray liquid, a spraying rate of 8 g / min, and an atomization pressure of 0.3 MPa. When the coating weight is 5 wt%, switch the spray liquid to a 5% ethyl cellulose ethanol solution. When the coating weight is 8 wt%, stop the process, remove the particles, and dry them to obtain the microbial activator.

2. The preparation method according to claim 1, characterized in that, The metal salt solution in S1 is a blend of FeSO4, ZnSO4, and MnCl2, and the mass ratio of FeSO4, ZnSO4, MnCl2, and water is (30-40):(5-10):1.5:

500.

3. The preparation method according to claim 1, characterized in that, The amino acid solution in S1 is a blend of glutamic acid and aspartic acid, and the mass ratio of glutamic acid, aspartic acid and water is 100:(60-80):

200.

4. The preparation method according to claim 1, characterized in that, The particle size of the amino acid chelated trace elements in S1 is 50-100 μm.

5. The preparation method according to claim 1, characterized in that, The mass ratio of amino acid chelated trace elements, glucose, fructose, water, ATP-γ-S, and coenzyme Q10 in S2 is 120:(30-50):(10-30):500:(1-5):1.

5.

6. The preparation method according to claim 1, characterized in that, The microcapsule size of the fast-acting layer in S2 is 450-800 μm.

7. The preparation method according to claim 1, characterized in that, The mass ratio of gelatinized corn starch, yeast peptone, calcium magnesium phytate, and PHA powder in S3 is 100:40:25:

35.

8. The preparation method according to claim 1, characterized in that, The sustained-release microspheres in S3 have a particle size of 1-1.2 mm.

9. The preparation method according to claim 1, characterized in that, The mass ratio of the sustained-release microspheres to the fast-acting microcapsules in S4 is 2:

1.

10. A microbial activator based on compound nutrient factors, characterized in that, It is prepared by the preparation method according to any one of claims 1-9.

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