Directional fermentation process for improving stability of SOD (superoxide dismutase)

By employing a targeted fermentation process involving strain domestication and multi-parameter synergistic regulation, the problem of poor stability in SOD fermentation production has been solved, achieving efficient extraction and low-energy SOD production, with significantly improved product stability and extraction efficiency.

CN120989022APending Publication Date: 2025-11-21SHANDONG LANDU BIOENGINEERING CO LTD
0 Cites 0 Cited by

Patent Information

Application Number
CN202511213072.9
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-08-28
Publication Date
2025-11-21

AI Technical Summary

Technical Problem

Existing SOD fermentation production suffers from poor stability, significant extraction losses, and weak process robustness, resulting in unsatisfactory product stability, high production costs, and large batch-to-batch quality fluctuations.

Method used

The method employs targeted strain domestication, multi-parameter synergistic regulation, and mild extraction techniques, including steps such as domesticating strains with gradient concentrations of reactive oxygen species inducers, multi-stage fermentation, low-temperature enzyme release, aqueous two-phase extraction, and freeze spray drying, to improve the thermal and storage stability of SOD.

Benefits of technology

Significantly improves the thermal and storage stability of SOD. After treatment at 60℃, the residual enzyme activity of the enzyme powder is ≥85%, and after storage at 4℃ for 6 months, the enzyme activity retention rate is ≥90%. The extraction efficiency and activity recovery rate are improved, the process energy consumption is reduced, and the product quality consistency is improved.

✦ Generated by Eureka AI based on patent content.
Patent Text Reader

Abstract

The invention discloses a directional fermentation process for improving SOD (superoxide dismutase) stability, and relates to the technical field of bioengineering. According to the process, stability-enhanced strains are screened through combination of an active oxygen inducer and high-temperature stress multi-round domestication; performing three-stage precise feed-supplementing fermentation by adopting a fermentation system, namely exponentially feeding a feed-supplementing liquid with a high nitrogen-carbon ratio in a growth stage, cooling to 25 DEG C in an induction stage, feeding an inducer and glycerol to maintain low residual sugar, and maintaining nutrition in a stable stage; carrying out low-temperature lysozyme-EDTA (Ethylene Diamine Tetraacetic Acid) mild wall breaking on the fermentation liquor through an enzyme release system, transferring into an aqueous two-phase extraction system, and extracting by adopting a PEG4000 / ammonium sulfate aqueous two-phase system; and performing ultrafiltration replacement on the crude enzyme liquid through a membrane separation system and a trehalose-containing buffer solution, adding gelatin, and performing freezing-spray drying through a drying system to obtain the high-stability SOD enzyme powder. According to the method, the intrinsic stability of SOD is synergistically improved in the whole process from a strain source to extraction and drying, and the thermal stability and long-term storage activity of the product are remarkably improved.
Need to check novelty before this filing date? Find Prior Art