A mild, moisturizing, soothing composition and uses thereof

Abstract

The present application relates to a kind of mild moisturizing soothing composition and its application, the composition includes the following weight parts of component: 5-10 parts of lactobacillus / fermentation product of algal extract, 2-5 parts of capparis zeylanica fruit extract, 3-5 parts of olive fruit oil and 10-15 parts of schisandra fruit extract-gentiana root extract, four synergistically improve the moisturizing soothing effect of composition.The schisandra fruit extract-gentiana root extract of the present application is biotransformed and synergistically metabolized by compound fermentation bacteria, significantly improves the utilization rate of active ingredients in schisandra fruit extract and gentiana root extract, and expands its efficacy range, can play the role of regulating inflammatory response, reinforcing skin barrier, solving the problem of eczema skin barrier, reducing scratching skin stinging discomfort, reducing eczema recurrence, solving the problem of dry peeling caused by indoor and outdoor temperature difference, dry area, reducing water loss and antioxidant damage and other multiple effects.

Description

Technical Field

[0001] This invention relates to the technical field of cosmetics, specifically to a mild moisturizing and soothing composition and its application. Background Technology

[0002] The integrity of the skin barrier function is one of the core elements ensuring healthy skin. At the same time, the strength of skin tolerance directly determines its ability to resist various external stimuli. Once the skin barrier function declines, it can lead to a series of problems such as excessive moisture loss, significantly increased sensitivity, and frequent inflammatory reactions. Conversely, reduced skin tolerance makes the skin more susceptible to damage from external irritants, allergens, and microorganisms.

[0003] Currently, the damage to the skin barrier function and the weakening of skin tolerance are becoming increasingly serious. Therefore, developing a cosmetic product that can strengthen the skin barrier, reduce scratching muscle irritation and discomfort, reduce eczema recurrence, and solve dryness and peeling problems caused by indoor and outdoor temperature differences and dry areas, and that can effectively repair the skin barrier, is of vital importance for improving skin health. Summary of the Invention

[0004] The purpose of this invention is to overcome the shortcomings of the prior art and provide a mild moisturizing and soothing composition and its application.

[0005] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0006] In a first aspect, the present invention provides a mild moisturizing and soothing composition comprising the following components in parts by weight: 5-10 parts of Lactobacillus / algae extract fermentation product, 2-5 parts of capernaum fruit extract, 3-5 parts of olive fruit oil, and 10-15 parts of Schisandra chinensis fruit extract-gentian root extract; wherein the preparation method of the Schisandra chinensis fruit extract-gentian root extract includes the following steps:

[0007] The compound plant extract, carbon source, inorganic salt and water are mixed and sterilized to obtain a sterilized culture medium; a compound fermentation bacteria are inoculated into the culture medium for fermentation culture, and the culture product is centrifuged, filtered and sterilized to obtain the Schisandra chinensis fruit extract-Gentiana scabra root extract; wherein, the compound plant extract is Schisandra chinensis fruit extract and Gentiana scabra root extract, and the compound fermentation bacteria are Bacillus cladosporioides, Rhodotorula glutinis and Lactococcus lactis subsp. milk fat with a live bacteria ratio of 1:(1.5-2):(1.5-2).

[0008] The specific effects of the Lactobacillus / algae extract fermentation product, Caprifolium fruit extract, olive fruit oil, and Schisandra chinensis fruit extract-Gentian root extract in the composition of the present invention are as follows:

[0009] Lactobacillus / algae extract fermentation products can inhibit the growth of harmful bacteria and maintain microbial balance; at the same time, the proteases, vitamins and organic matter (such as unsaturated fatty acids) in its fermentation products can soften the stratum corneum, promote epidermal renewal and enhance the skin barrier function.

[0010] Caprifolia fruit extract is rich in flavonoids, alkaloids and other components, which can quickly relieve discomfort such as stinging and itching. Moreover, the antioxidant activity of the flavonoids it contains can help eliminate free radicals produced by skin inflammation, reduce oxidative damage and further alleviate skin stress response.

[0011] Olive fruit oil is rich in phenolic compounds and unsaturated fatty acids, which can effectively reduce inflammation, repair the skin's lipid barrier, and moisturize dry skin.

[0012] Schisandra chinensis fruit extract and Gentiana scabra root extract are compound plant extracts obtained through compound fermentation by three strains (Bacillus clausti, Rhodotorula glutinis, and Lactococcus lactis subsp. lactis). The specific functions of each strain are as follows:

[0013] Schisandra chinensis fruit extract contains lignans such as schisandrin A and schisandrin B. These components have large molecular weights and are highly lipid-soluble, making them difficult for the skin to absorb directly. However, *Lactococcus lactis* subsp. *lactolipum* can secrete esterases to hydrolyze the ester bonds of lignans, converting them into smaller aglycones and improving absorption. Some polysaccharides in Schisandra chinensis fruit extract may cause a sticky feeling on the skin; *Lactococcus lactis* subsp. *lactolipum* polysaccharide enzymes can degrade them into oligosaccharides, preserving moisturizing properties, improving the skin feel, and reducing the potential burden of polysaccharides on the skin barrier. The core active ingredient in Gentiana scabra root extract is gentiopicroside (an iridoid glycoside). *Bacillus clausti* glycosidases can hydrolyze it into gentiopicroside aglycone, which is not only smaller and more easily penetrated but also reduces the potential slight irritation from the original ingredient. *Rhodotorula glutinis* can secrete polyphenol oxidase and peroxidase, which can convert the phenolic acids in Schisandra chinensis fruit and Gentiana scabra root extracts into quinone derivatives with greater antioxidant activity. Meanwhile, the acidic environment produced during the fermentation process of the three strains can disrupt the plant cell wall structure, increasing the release of active ingredients such as saponins and flavonoids that were originally encased in the cell wall, further enhancing the soothing effect of Schisandra chinensis fruit extract-Gentiana scabra root extract.

[0014] Preferably, the mass ratio of the Schisandra chinensis fruit extract to the Gentiana scabra root extract is 1:(0.3-0.8).

[0015] Preferably, the culture medium comprises the following components in parts by weight: 22-30 parts of compound plant extract, 0.5-1.5 parts of carbon source, 0.03-0.7 parts of inorganic salt, and 80-100 parts of water.

[0016] Preferably, the inoculum size of the compound fermentation bacteria is 2-4% v / v of the culture medium, and the viable count of the compound fermentation broth is 1×10⁻⁶.9 -2×10 10 CFU / mL.

[0017] Preferably, the carbon source includes at least one of maltose, sucrose, and glucose.

[0018] Preferably, the inorganic salt is at least one selected from dipotassium hydrogen phosphate, potassium dihydrogen phosphate, and sodium chloride.

[0019] Preferably, in the preparation method of the Schisandra chinensis fruit extract-Gentiana scabra root extract, the fermentation temperature is 28-30℃ and the time is 16-20h.

[0020] Preferably, in the preparation method of the Schisandra chinensis fruit extract-Gentiana scabra root extract, the centrifugation speed is 10000-15000 rpm and the time is 5-10 min.

[0021] In a second aspect, the present invention provides the use of the mild moisturizing and soothing composition of the first aspect in the preparation of cosmetics.

[0022] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0023] (1) The mild moisturizing and soothing composition of the present invention contains caperca zedoaria fruit extract to quickly relieve stinging and itching, lactobacillus / algae extract fermentation product to reduce inflammation and stabilize the microenvironment, schisandra fruit extract-gentian root extract to deeply repair the skin barrier, and olive fruit oil to lock in moisture and protect and consolidate the results. The four ingredients work together to improve the moisturizing and soothing effect of the composition.

[0024] (2) The Schisandra chinensis fruit extract and Gentiana scabra root extract of the present invention undergo biotransformation and synergistic metabolism through compound fermentation bacteria, which significantly improves the utilization rate of active ingredients in Schisandra chinensis fruit extract and Gentiana scabra root extract and expands their efficacy range. Lactococcus lactis subsp. lactis can secrete esterases to hydrolyze the large molecular lignans in Schisandra chinensis fruit extract into small molecular aglycones to improve absorption rate. Moreover, the polysaccharide enzymes contained in Lactococcus lactis subsp. lactis can also degrade some of its polysaccharides into oligosaccharides, retaining moisturizing properties, improving skin feel, and reducing the burden on the skin barrier. Glycosidases in Bacillus clausti can hydrolyze gentiopicrin in Gentiana scabra root extract into gentiopicrin aglycone, which is easily penetrated and has low irritation. Rhodotorula glutinis can secrete enzymes to convert phenolic acids in Schisandra chinensis fruit and Gentiana scabra root extracts into quinone derivatives with greater antioxidant activity. The acidic environment produced during the fermentation of the three strains can disrupt the structure of plant cell walls, increasing the release of active ingredients such as saponins and flavonoids that are originally encased in the cell walls. Therefore, the fermented product can play multiple roles, such as regulating inflammatory responses, protecting and repairing the skin barrier, reducing moisture loss, and resisting oxidative damage. This can fundamentally solve the skin barrier problem of eczema, reduce the stinging and discomfort of scratching the skin, reduce the recurrence of eczema, and solve the dryness and peeling problems caused by indoor and outdoor temperature differences and dry areas. Detailed Implementation

[0025] To better illustrate the purpose, technical solution, and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0026] The sources of raw materials and preparation methods used in the following examples and comparative examples are as follows:

[0027] Lactobacillus / algae extract fermentation product: Manufacturer is Hemei Biotechnology, trade name is Rufuyin;

[0028] Caprifolia fruit extract: manufactured by Jiakai Biotechnology, trade name Gmoist CS;

[0029] Olive fruit oil: Manufacturer: Lipovol™ O; Model: Lipovol™ O;

[0030] Schisandra chinensis fruit extract: Manufacturer: Droca, trade name: Schisandra chinensis extract;

[0031] Gentian root extract: prepared according to the method for preparing gentian anti-irritant factor and the plant extraction apparatus in Example 1 of CN107137261B;

[0032] Bacillus clausti: Manufacturer: Beijing BioBio Biotechnology Co., Ltd., Product No.: bio-103964;

[0033] Red yeast rice: Manufacturer is Beijing Bio-Bio Biotechnology Co., Ltd., product number is Bio-63338;

[0034] Lactococcus lactis subsp. milk fat: Manufacturer is Mingzhou Biotechnology, product number BMZ139666.

[0035] Before use, each of the above fermentation bacteria should be activated and combined according to the required ratio of live bacteria.

[0036] Unless otherwise specified, all other materials and reagents used in the examples are commercially available.

[0037] Example 1

[0038] A mild moisturizing and soothing composition comprising the following components in parts by weight: 8 parts Lactobacillus / algae extract fermentation product, 3 parts Caprifolium fruit extract, 4 parts Olive fruit oil, and 13 parts Schisandra chinensis fruit extract-Gentiana root extract; wherein the preparation method of the Schisandra chinensis fruit extract-Gentiana root extract includes the following steps:

[0039] The compound plant extract, carbon source, inorganic salts, and water were mixed and sterilized to obtain a sterilized culture medium. A compound fermentation bacteria were then inoculated into the culture medium for fermentation. The culture product was centrifuged, filtered, and sterilized to obtain the Schisandra chinensis fruit extract-Gentiana scabra root extract. The culture medium comprises the following components by weight: 25 parts compound plant extract, 1 part carbon source, 0.5 parts inorganic salts, and 95 parts water. The compound plant extract is a mixture of Schisandra chinensis fruit extract and Gentiana scabra root extract in a mass ratio of 1:0.5. The carbon source is sucrose and glucose in a mass ratio of 1:1. The inorganic salts are potassium dihydrogen phosphate and sodium chloride in a mass ratio of 1:1. The inoculum size of the compound fermentation bacteria is 3% v / v of the culture medium, and the viable count of the compound fermentation broth is 1 × 10⁻⁶. 10 The CFU / mL of the compound fermentation bacteria consists of Bacillus cladei, Rhodotorula glutinis, and Lactococcus lactis subsp. milk fat in a live bacteria ratio of 1:1.6:1.8; the fermentation temperature is 29℃ and the time is 18h; the centrifugation speed is 12000rpm and the time is 8min; and the filtration is performed using a 0.45μm filter membrane.

[0040] Example 2

[0041] A mild moisturizing and soothing composition comprising the following components in parts by weight: 5 parts Lactobacillus / algae extract fermentation product, 2 parts Caprifolium fruit extract, 3 parts Olive fruit oil, and 10 parts Schisandra chinensis fruit extract-Gentiana root extract; wherein the preparation method of the Schisandra chinensis fruit extract-Gentiana root extract includes the following steps:

[0042] The compound plant extract, carbon source, inorganic salts, and water were mixed and sterilized to obtain a sterilized culture medium. A compound fermentation bacteria were inoculated into the culture medium for fermentation. The culture product was centrifuged, filtered, and sterilized to obtain the Schisandra chinensis fruit extract-Gentiana scabra root extract. The culture medium comprises the following components by weight: 22 parts compound plant extract, 0.5 parts carbon source, 0.03 parts inorganic salts, and 80 parts water. The compound plant extract is a mixture of Schisandra chinensis fruit extract and Gentiana scabra root extract in a mass ratio of 1:0.3. The carbon source is maltose, and the inorganic salts are dipotassium hydrogen phosphate and sodium chloride in a mass ratio of 1:1. The inoculum size of the compound fermentation bacteria is 2% v / v of the culture medium, and the viable count of the compound fermentation broth is 2 × 10⁻⁶. 10 The CFU / mL of the compound fermentation bacteria consists of Bacillus cladei, Rhodotorula glutinis, and Lactococcus lactis subsp. milk fat in a live bacteria ratio of 1:1.5:2; the fermentation temperature is 30℃ and the time is 16h; the centrifugation speed is 10000rpm and the time is 10min; and the filtration is performed using a 0.45μm filter membrane.

[0043] Example 3

[0044] A mild moisturizing and soothing composition comprising the following components in parts by weight: 10 parts of Lactobacillus / algae extract fermentation product, 5 parts of Caprifolium fruit extract, 5 parts of olive fruit oil, and 15 parts of Schisandra chinensis fruit extract-Gentiana root extract; wherein the preparation method of the Schisandra chinensis fruit extract-Gentiana root extract includes the following steps:

[0045] The compound plant extract, carbon source, inorganic salts, and water were mixed and sterilized to obtain a sterilized culture medium. A compound fermentation bacteria were then inoculated into the culture medium for fermentation. The culture product was centrifuged, filtered, and sterilized to obtain the Schisandra chinensis fruit extract-Gentiana scabra root extract. The culture medium comprises the following components by weight: 30 parts compound plant extract, 1.5 parts carbon source, 0.7 parts inorganic salts, and 100 parts water. The compound plant extract is a mixture of Schisandra chinensis fruit extract and Gentiana scabra root extract in a mass ratio of 1:0.8. The carbon source is maltose and glucose in a mass ratio of 1:1. The inorganic salts are dipotassium hydrogen phosphate, potassium dihydrogen phosphate, and sodium chloride in a mass ratio of 1:1:1. The inoculum size of the compound fermentation bacteria is 4% v / v of the culture medium, and the viable count of the compound fermentation broth is 1 × 10⁻⁶. 9 The CFU / mL of the compound fermentation bacteria consists of Bacillus cladei, Rhodotorula glutinis, and Lactococcus lactis subsp. milk fat in a live bacteria ratio of 1:2:1.5; the fermentation temperature is 28℃ and the time is 20h; the centrifugation speed is 15000rpm and the time is 5min; and the filtration is performed using a 0.45μm filter membrane.

[0046] Example 4

[0047] The only difference between Example 4 and Example 1 is that the amount of compound plant extract added remains the same, and the compound plant extract is a schisandra fruit extract and a gentian root extract with a mass ratio of 0.5:1.

[0048] Comparative Example 1

[0049] The only difference between Comparative Example 1 and Example 1 is that a compound plant extract is used to replace an equal amount of Schisandra chinensis fruit extract and Gentiana scabra root extract. The compound plant extract is a mixture of Schisandra chinensis fruit extract and Gentiana scabra root extract in a mass ratio of 1:0.5.

[0050] Comparative Example 2

[0051] The only difference between Comparative Example 2 and Example 1 is that the number of viable bacteria in the compound fermentation bacteria remains unchanged, Bacillus clausii is not added, and Rhodotorula glutinis and Lactococcus lactis subsp. milk fat with a viable bacteria ratio of 1.6:1.8 are used to make up for the missing amount.

[0052] Comparative Example 3

[0053] The only difference between Comparative Example 3 and Example 1 is that the number of viable bacteria in the compound fermentation bacteria remains unchanged, no Rhodotorula glutinis is added, and Bacillus clavatum and Lactococcus lactis subsp. milk fat with a viable bacteria ratio of 1:1.8 are used to make up for the missing amount.

[0054] Comparative Example 4

[0055] The only difference between Comparative Example 4 and Example 1 is that the number of viable bacteria in the compound fermentation bacteria remains unchanged, no *Lactococcus lactis* subsp. milk fat is added, and *Bacillus clavatum* and *Rhodotorula glutinis* with a viable bacteria ratio of 1:1.6 are used to make up for the missing amount.

[0056] Comparative Example 5

[0057] The only difference between Comparative Example 5 and Example 1 is that the number of viable bacteria in the compound fermentation bacteria remains unchanged, and the ratio of viable bacteria of Bacillus clausii, Rhodotorula glutinis and Lactococcus lactis subsp. milk fat is 1.6:1:1.8.

[0058] Comparative Example 6

[0059] The only difference between Comparative Example 6 and Example 1 is that the number of viable bacteria in the compound fermentation bacteria remains unchanged, and the ratio of viable bacteria of Bacillus clausii, Rhodotorula glutinis and Lactococcus lactis subsp. milk fat is 1.8:1.6:1.

[0060] Performance testing

[0061] (1) Superoxide anion free radical scavenging experiment

[0062] Preparation of sample reaction solutions: The mild moisturizing and soothing compositions of Examples 1-4 and Comparative Examples 1-6 were dissolved in 30% v / v ethanol aqueous solution to ensure that the final concentration of the mild moisturizing and soothing compositions reached 5% (v / v), and these were used as the test samples. Next, 4.5 mL of Tris-HCl buffer solution with a concentration of 0.05 mol / L and a pH of 8.2 was taken and preheated in a water bath at 25°C for 20 minutes. Subsequently, 1 mL of the test sample and 0.4 mL of pyrogallol solution with a concentration of 25 mmol / L were added, and the mixture was thoroughly mixed. The reaction was continued in a water bath at 25°C for 5 minutes. Finally, 1.0 mL of HCl solution with a concentration of 8 mol / L was added to terminate the reaction.

[0063] Preparation of the control group reaction solution: The preparation process is the same as that of the sample group reaction solution, except that 1 mL of the test sample is replaced with 1 mL of 30% v / v ethanol aqueous solution.

[0064] After calibrating the instrument using Tris-HCl buffer as a reference, the absorbance of the two reaction solutions was measured at a wavelength of 299 nm. The scavenging rate of superoxide anions was calculated using the formula "scavenging rate % = [(A0 - Ax) / A0] × 100%", where A0 represents the absorbance of the control group and Ax represents the absorbance of the sample group. Each experiment was performed in triplicate, and the final result was the average. Specific data are shown in Table 1. Superoxide anion radical scavenging rate refers to the ability of an antioxidant to scavenge superoxide anion radicals, usually expressed as a scavenging rate. A higher scavenging rate indicates a stronger ability of the antioxidant to scavenge superoxide anion radicals, meaning a better antioxidant effect.

[0065] (2) Hyaluronidase inhibition experiment

[0066] Prepare a hyaluronidase ELISA kit and perform the following tests according to the kit's instructions:

[0067] ① The mild moisturizing and soothing compositions of Examples 1-4 and Comparative Examples 1-6 were mixed with PBS buffer solution (pH 7) at a mass ratio of 1:30 to prepare the test samples;

[0068] ② Set up blank wells, control wells, and sample wells. Blank wells: do not add hyaluronidase or test samples; control wells: add 50 μL of hyaluronidase (concentration of 1000 U / L), do not add test samples; sample wells: add 50 μL of hyaluronidase (concentration of 1000 U / L) and 50 μL of each group of test samples; after adding the materials, place the control wells and sample wells in a 37℃ incubator for 10 min, and wash the plate 5 times.

[0069] ③ Except for the blank wells, add 100 μL of horseradish peroxidase-labeled detection antibody to the control wells and sample wells respectively, seal each well with sealing film, and incubate at 37℃ for 60 min.

[0070] ④ Discard the liquid, pat dry with absorbent paper, and wash the plate 3 times;

[0071] ⑤ Add 50 μL of substrate A and 50 μL of substrate B to each well and incubate at 37 °C in the dark for 15 min; wherein, substrate A is a 0.01% hydrogen peroxide solution and substrate B is a 0.1% tetramethylbenzidine (TMB) solution.

[0072] ⑥ Add 50 μL of stop solution (i.e., 2 mol / L dilute H2SO4) to each well, and measure the OD value of each well at a wavelength of 450 nm within 15 min. Calculate the inhibition rate according to the formula: "Inhibition rate (%) = (OD value of each well) / (2 mol / L dilute H2SO4) / 2 mol / L dilute H2SO4". 对照孔 -OD 样品孔 ) / (OD 对照孔 -OD 空白孔 The percentage was calculated as follows: (×100%). Each experiment included three replicates, and the final result was the average of these three replicates. Specific data are shown in Table 1. Hyaluronidase is a specific lysin that breaks down hyaluronic acid (HA). Hyaluronic acid is a major component of the skin's extracellular matrix, possessing strong water-absorbing and adhesive properties, maintaining skin moisture and elasticity, and protecting the skin barrier function. However, when hyaluronidase is overactive, it leads to hyaluronic acid degradation, damage to the skin barrier, and exacerbation of inflammatory responses. A higher hyaluronidase inhibition rate indicates a stronger inhibitory effect of the active ingredient in the composition on hyaluronidase, thus more effectively reducing the breakdown of hyaluronic acid and protecting and repairing the skin barrier.

[0073] (3) Inflammatory factor inhibition experiment

[0074] This experiment used RAW264.7 macrophages as the research subject, and stimulated the cells with lipopolysaccharide (LPS) (bacterial endotoxin) to construct a cellular inflammation model. The specific procedures were as follows: macrophages were spaced at 1.0 × 10⁶ cells per well. 4 The samples were seeded at a density of [number] cells per well in a 12-well plate, and then incubated for 24 hours at 37°C with 5% CO2 aeration. After 24 hours, 4 μL of a 1% (v / v) dilution solution (obtained by diluting the mild moisturizing and soothing compositions of Examples 1-4 and Comparative Examples 1-6 with 30% v / v ethanol aqueous solution) was added to each well. The plate was then returned to the incubator at 37°C with 5% CO2 aeration for another 2 hours. Afterward, LPS was added to achieve a final concentration of 1 μg / mL in the dilution solution, and the plate was incubated for another 16 hours at 37°C with 5% CO2 aeration. This group was designated as the sample group.

[0075] Meanwhile, two control groups were set up: control group 1 did not add the diluent of the mild moisturizing and soothing composition, but only added LPS; control group 2 did not add LPS, but only added the diluent of the mild moisturizing and soothing composition.

[0076] After the experiment, the supernatant from each well was collected and centrifuged. Then, the release of the pro-inflammatory cytokine TNF-α from RAW264.7 macrophages was detected using an ELISA kit. The TNF-α inhibition rate was calculated using the formula: "TNF-α inhibition rate % = (Inflammatory cytokine concentration in control group 1 - Inflammatory cytokine concentration in sample group) / (Inflammatory cytokine concentration in control group 1 - Inflammatory cytokine concentration in control group 2) × 100%". Specific data are shown in Table 1. The TNF-α inhibition rate refers to the degree to which the inhibitor inhibits TNF-α activity. A higher inhibition rate indicates a stronger inhibitory effect on TNF-α, i.e., a stronger anti-inflammatory ability. Therefore, the TNF-α inhibition rate can directly reflect the anti-inflammatory ability of a substance, thus demonstrating its soothing effect.

[0077] Table 1 Performance test results of each group of samples

[0078] Group / Performance Superoxide anion scavenging rate (%) Hyaluronidase inhibition rate (%) TNF-α inhibition rate (%) Example 1 81.70 69.66 83.92 Example 2 80.51 67.42 83.26 Example 3 81.26 69.87 84.15 Example 4 76.10 64.63 81.47 Comparative Example 1 54.06 39.47 55.68 Comparative Example 2 63.81 50.24 63.33 Comparative Example 3 59.24 45.16 61.02 Comparative Example 4 61.50 47.65 65.79 Comparative Example 5 70.58 57.41 72.60 Comparative Example 6 71.41 58.55 73.23

[0079] As shown in Table 1, the superoxide anion scavenging rate, hyaluronidase inhibition rate, and TNF-α inhibition rate of Example 4 were all lower than those of Example 1. This indicates that the antioxidant, skin barrier repair, and anti-inflammatory soothing effects of Example 4 were lower than those of Example 1. This may be because the proportion of gentian root extract was increased and the proportion of schisandra fruit extract was decreased in Example 4, which affected the generation and interaction of active ingredients during fermentation. Therefore, when the ratio of schisandra fruit extract to gentian root extract is within the preferred range of 1:(0.3-0.8), the content of active substances (such as lignans and gentianin) of the two components is well matched, making it easier for the compound fermentation bacteria to convert them into small molecule active ingredients, and the composition has better antioxidant, skin barrier repair, and anti-inflammatory soothing effects.

[0080] Combining the data from Example 1 and Comparative Examples 1-4, it is evident that the data from Comparative Example 1 (unfermented) are significantly lower than those from Example 1. This indicates that microbial fermentation is a crucial step in enhancing the activity of plant extracts. Fermentation not only breaks down cell walls to release more active ingredients (such as gentiopicrin and schisandrin), but also transforms them into more easily absorbed and more active small molecules, and generates new active substances (such as GABA analogs and antimicrobial peptides). Compared to Example 1, the fermented products of Comparative Examples 2-4 all lacked one type of fermenting bacteria, resulting in a significant decrease in the antioxidant and soothing properties of the compositions. This may be because the absence of a certain type of fermenting bacteria disrupts the synergistic effect between bacterial species, affecting the generation of active ingredients and the diversity of metabolites during fermentation, thereby reducing the antioxidant, skin barrier repair, and anti-inflammatory soothing abilities.

[0081] Based on the data from Example 1 and Comparative Examples 5-6, it can be seen that compared with Example 1, the antioxidant and soothing anti-inflammatory effects of Comparative Examples 5-6 were reduced. This may be because: Comparative Example 5 reduced the proportion of Rhodotorula glutinis, leading to a decrease in polyphenol conversion efficiency and a reduction in antioxidant components; Comparative Example 6 reduced the proportion of Lactococcus lactis subsp. lipofus, resulting in insufficient organic acid secretion, an unbalanced fermentation environment, and a decrease in overall conversion efficiency. Therefore, fermentation achieves optimal results when the ratio of viable bacteria of Bacillus cladei, Rhodotorula glutinis, and Lactococcus lactis subsp. lipofus is in the range of 1:(1.5-2):(1.5-2).

[0082] In summary, the composition of the present invention has multiple effects, such as regulating inflammatory response, protecting and repairing the skin barrier, reducing moisture loss, and resisting oxidative damage.

[0083] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A mild moisturizing and soothing composition, characterized in that, The product comprises the following components in parts by weight: 5-10 parts of Lactobacillus / algae extract fermentation product, 2-5 parts of Caprifolium fruit extract, 3-5 parts of olive fruit oil, and 10-15 parts of Schisandra chinensis fruit extract-Gentiana root extract; wherein the preparation method of the Schisandra chinensis fruit extract-Gentiana root extract includes the following steps: The compound plant extract, carbon source, inorganic salt and water are mixed and sterilized to obtain a sterilized culture medium; a compound fermentation bacteria are inoculated into the culture medium for fermentation culture, and the culture product is centrifuged, filtered and sterilized to obtain the Schisandra chinensis fruit extract-Gentiana scabra root extract; wherein, the compound plant extract is Schisandra chinensis fruit extract and Gentiana scabra root extract, the compound fermentation bacteria are Bacillus cladosporioides, Rhodotorula glutinis and Lactococcus lactis subsp. milk fat with a live bacteria ratio of 1:(1.5-2):(1.5-2); the mass ratio of Schisandra chinensis fruit extract to Gentiana scabra root extract is 1:(0.3-0.8).

2. The mild moisturizing and soothing composition as described in claim 1, characterized in that, The culture medium comprises the following components in parts by weight: 22-30 parts of compound plant extract, 0.5-1.5 parts of carbon source, 0.03-0.7 parts of inorganic salt, and 80-100 parts of water.

3. The mild moisturizing and soothing composition as described in claim 1, characterized in that, The inoculation amount of the compound fermentation bacteria is 2-4% v / v of the culture medium, and the viable count of the compound fermentation broth is 1×10⁻⁶. 9 -2×10 10 CFU / mL.

4. The mild moisturizing and soothing composition as described in claim 1, characterized in that, The carbon source includes at least one of maltose, sucrose, and glucose; and / or, the inorganic salt is at least one of dipotassium hydrogen phosphate, potassium dihydrogen phosphate, and sodium chloride.

5. The mild moisturizing and soothing composition as described in claim 1, characterized in that, In the preparation method of Schisandra chinensis fruit extract-Gentiana scabra root extract, the fermentation temperature is 28-30℃ and the time is 16-20h.

6. The mild moisturizing and soothing composition as described in claim 1, characterized in that, In the preparation method of Schisandra chinensis fruit extract-Gentiana scabra root extract, the centrifugation speed is 10000-15000 rpm and the time is 5-10 min.

7. The use of the mild moisturizing and soothing composition according to any one of claims 1-6 in the preparation of cosmetics.