Method for highly converting algae organic solid waste into biological carbon source
By disrupting algal cell walls through electrochemical pretreatment and combining it with anaerobic fermentation technology, the problem of low biodegradability of algal organic solid waste for resource utilization has been solved, achieving efficient conversion into a biological carbon source, reducing costs and harmful byproducts.
Patent Information
- Application Number
- CN202511246966.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-09-02
- Publication Date
- 2025-12-09
AI Technical Summary
In existing technologies, the anaerobic digestion process of algal organic solid waste is limited by the difficulty in breaking down algal cell walls, resulting in low biodegradability and inefficient resource utilization. Furthermore, traditional methods may produce toxic and harmful byproducts.
Electrochemical pretreatment of algal organic solid waste was adopted, with the voltage controlled at 2.5–10V to destroy the cell walls of algae. Subsequently, under anaerobic fermentation conditions, volatile fatty acids were produced by inoculating microorganisms as a biocarbon source.
It improves the biodegradability of algal organic solid waste, significantly increases the yield of volatile fatty acids, reduces costs, avoids the generation of chemical byproducts, is environmentally friendly, and improves the efficiency of resource utilization.
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Figure CN121087113A_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The present application belongs to the technical field of environmental protection and resource utilization, and particularly relates to a method for high conversion of algal organic solid waste into a biological carbon source. BACKGROUND
[0002] The prior art focuses on enriching a substrate with only one kind of algal cells, then treating the substrate, and then performing anaerobic fermentation to produce a biological carbon source. As the earliest photosynthetic oxygen-releasing organism, cyanobacteria can improve soil fertility through nitrogen fixation and increase crop yield. Some cyanobacteria have become food for people and raw materials for producing chemicals through genetic modification, and have high resource utilization potential.
[0003] However, in actual water bodies, the overabundance of nitrogen and phosphorus in the water body leads to water eutrophication, resulting in the proliferation of cyanobacteria and the outbreak of water blooms. Algae consume dissolved oxygen in the water body while releasing toxic and harmful substances, causing water quality deterioration and seriously threatening people's life and production safety. As can be seen, the actual water bloom water body and the algal residue and algal sludge produced by physical salvage are a complete ecosystem, which not only contains a variety of cyanobacteria, but also contains a variety of other organisms such as bacteria, as well as a large amount of inorganic salts and organic components.
[0004] Studies have shown that anaerobic digestion, as the main biological disposal method of organic solid waste, can also produce CH4 and CO2 from algae. However, due to the difficulty in breaking down the cell wall of algae and the limited release of intracellular organic compounds, the biodegradability is low, so anaerobic digestion with algae as substrate is usually limited. Traditional anaerobic digestion conditions are not conducive to the resource utilization of algae. SUMMARY
[0005] In order to solve the defects in the prior art, the present application provides a method for high conversion of algal organic solid waste into a biological carbon source. Without adding any reagent, the method performs electrochemical pretreatment on the algal organic solid waste, and the cell wall of the algal organic solid waste after electrochemical treatment is largely destroyed, making it easier to be utilized and resourceized by anaerobic fermentation. By regulating the anaerobic fermentation conditions, volatile fatty acids that are easier to utilize and have higher energy conversion rates are produced during the fermentation process, providing a new path and new ideas for the development and application of algal resource utilization technology.
[0006] In order to achieve the above-mentioned purpose, the present application adopts the following technical solutions:
[0007] The present application provides a method for high conversion of algal organic solid waste into a biological carbon source, comprising the following steps:
[0008] Electrochemically pretreating the algal organic solid waste to obtain a fermentation substrate;
[0009] The fermentation substrate is added to the anaerobic fermentation tank, and then the inoculated microorganism is added, oxygen in the anaerobic fermentation tank is removed, and anaerobic fermentation is performed;
[0010] The fermentation liquid after anaerobic fermentation is subjected to solid-liquid separation to obtain a biological carbon source.
[0011] In the electrochemical pretreatment, the control voltage is 2.5-10 V.
[0012] Preferably, the electrochemical pretreatment of the algal organic solid waste specifically comprises:
[0013] The algal organic solid waste is placed in an electrolytic tank, an anode and a cathode are respectively placed in the electrolytic tank, and the anode and the cathode are respectively electrically connected to the positive electrode and the negative electrode of a power supply to perform electrolysis.
[0014] In the electrolysis, the control electrolysis voltage is 2.5-10 V, and the electrolysis time is 30-120 min.
[0015] Preferably, the solid suspended substance concentration in the algal organic solid waste is 10-200 g / L.
[0016] Preferably, the Cl- content in the algal organic solid waste is 100-150 mg / L. -
[0017] Preferably, the preparation method of the inoculated microorganism is:
[0018] The sludge is placed in an anaerobic fermentation tank and subjected to anaerobic fermentation at 35-36°C for 30-40 d, and after sedimentation, the supernatant is removed to obtain the inoculated microorganism; wherein the MLSS in the sludge is 8-16 g / L, and the MLVSS is 6.5-14 g / L.
[0019] Preferably, in the anaerobic fermentation, the control temperature is 33-38°C, and the time is 6-16 d.
[0020] Preferably, the oxygen in the anaerobic fermentation tank is removed by introducing nitrogen into the anaerobic fermentation tank, and the nitrogen introduction time is 5-10 min.
[0021] Preferably, the fermentation liquid after anaerobic fermentation is subjected to centrifugal solid-liquid separation to obtain a biological carbon source.
[0022] In the centrifugal solid-liquid separation, the centrifugal speed is 3000-8000 r / min, and the centrifugal time is 5-8 min.
[0023] Preferably, the fermentation substrate is added to the anaerobic fermentation tank, then the inoculated microorganism is added, the pH is adjusted to 7-8, the oxygen in the anaerobic fermentation tank is removed, and anaerobic fermentation is performed.
[0024] Preferably, the anode is a titanium plate loaded with a ruthenium-iridium oxide coating, and the cathode is a titanium plate.
[0025] The method for high conversion of algal organic solid waste into a biological carbon source has the following effects compared with the prior art:
[0026] The method for high conversion of algal organic solid waste into a biological carbon source of the present application directly uses an electrochemical method to treat algal organic solid waste without additional addition of reagents, not only improves the biodegradability of algal organic solid waste, further improves the efficient resource utilization of algal organic solid waste, but also reduces the cost and the risk of by-products caused by additional addition of reagents; the electrochemical method has the characteristics of high efficiency, good universality, high cleanliness and environmental friendliness, can remove algae in water without producing toxic and harmful by-products, can ensure the safety of water and the safety of subsequent natural ecology of water, and also avoids the use of other additional reagents; the yield of volatile fatty acids produced in the fermentation process of the pretreated substrate is significantly improved, and the highest yield reaches 5541 mg / L, which is 19% higher than that of single algal enrichment. BRIEF DESCRIPTION OF DRAWINGS
[0027] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the drawings needed to be used in the embodiments or the prior art description will be briefly introduced. Obviously, the drawings in the following description are only some embodiments of the present application, and those skilled in the art can obtain other drawings according to these drawings without creative labor.
[0028] Figure 1 The volatile fatty acid yield comparison chart of the supernatant in Example 1 and Comparative Example 1 of the present application. DETAILED DESCRIPTION
[0029] In order to facilitate the understanding of the present application, the present application will be described more fully below in conjunction with specific embodiments. The specific embodiments given in the specific embodiments are preferred embodiments of the present application. However, the present application can be realized in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the present application more thorough and comprehensive.
[0030] The order of description of the following embodiments is not intended to imply a preference over other embodiments. Additionally, in the description of the application, the term "including" means "including but not limited to". Various embodiments of the application can exist in a range of forms; it should be understood that the description in the form of a range is merely for the convenience and brevity, and should not be understood as a hard restriction on the scope of the application; therefore, it should be considered that the range described has been specifically disclosed all possible sub-ranges and single values within the range. For example, it should be considered that the range description from 1 to 6 has specifically disclosed sub-ranges, such as from 1 to 3, from 1 to 4, from 1 to 5, from 2 to 4, from 2 to 6, from 3 to 6, etc., as well as single numbers within the range, such as 1, 2, 3, 4, 5 and 6, regardless of the range. In addition, whenever a numerical range is indicated in this document, it refers to any cited number (fraction or integer) within the indicated range.
[0031] The application provides a method for high conversion of algal organic solid waste into a biological carbon source, comprising the following steps:
[0032] S1, electrochemically pretreating the algal organic solid waste to obtain a fermentation substrate;
[0033] S2, adding the fermentation substrate to an anaerobic fermentation tank, adding inoculated microorganisms, removing oxygen in the anaerobic fermentation tank and anaerobic fermentation;
[0034] S3, solid-liquid separation of the fermentation liquid after anaerobic fermentation to obtain a biological carbon source;
[0035] Wherein, the control voltage during electrochemical pretreatment is 2.5-10V.
[0036] The purpose of the application is to promote the breakage of algae and efficient resource recovery without adding reagents, and a method for efficient targeted conversion of algal organic solid waste in water into a biological carbon source without reagents is provided; comprising the following steps: obtaining algal organic solid waste from natural lake water, directly electrochemically pretreating the algal organic solid waste to form a fermentation substrate; adding the fermentation substrate to an anaerobic fermentation tank, adding inoculated microorganisms, then blowing nitrogen to remove oxygen, sealing the anaerobic fermentation tank and transferring to a constant temperature incubator for anaerobic fermentation to produce volatile fatty acids from algal organic matter; solid-liquid separation of the fermentation liquid after fermentation to obtain a biological carbon source rich in volatile fatty acids.
[0037] The basic principle of the drug-free electrochemical pretreatment in the application for promoting efficient targeted conversion of algal organic solid waste into a biological carbon source is that high-oxidizing substances such as hypochlorite and free radicals generated by electrochemical pretreatment perform wall-breaking on algal cell walls, so that the algal cells are broken or ruptured, and the organic matter in the cells is more easily released, improving biodegradability and promoting resource utilization. The yield of volatile fatty acids produced during fermentation of the pretreated substrate is significantly improved, reaching a maximum of 5541 mg / L, which is 19% higher than that of the volatile fatty acids produced after enrichment of single algae. In addition, electrochemical pretreatment has the advantages of cleanliness and high efficiency, and does not affect the environment.
[0038] In some embodiments, the electrochemical pretreatment of algal organic solid waste specifically includes:
[0039] The algal organic solid waste is placed in an electrolytic tank, and an anode and a cathode are respectively placed in the electrolytic tank, and the anode and the cathode are respectively electrically connected to the positive and negative poles of a power supply for electrolysis.
[0040] The electrolysis voltage is controlled to be 2.5-10V, and the electrolysis time is controlled to be 30-120min.
[0041] In some embodiments, the algal (such as blue-green algae) organic solid waste is obtained from natural lake water. Specifically, algal sludge obtained from natural lake water is taken out and mixed with pure water to prepare a solid suspension with a concentration of 10-200g / L, which is the algal organic solid waste. The organic content accounts for 80%-90%, and the inorganic content accounts for 10%-20%. The organic components mainly include polysaccharides, proteins, lipids and other substances, and also contain aggregated algal cells, including extracellular aggregated extracellular organic matter and living cells mainly composed of cell walls, cell membranes and cytoplasm. The inorganic components include inorganic salts such as potassium salt (such as K + ), which is a nutrient element necessary for algal growth and participates in metabolic regulation, possibly in the form of KCl, sodium salt (such as Na + ), which is a common ion in lake water, possibly in the form of NaCl, which maintains osmotic pressure together with Cl-, calcium salt (such as Ca 2+ ), which participates in algal cell wall synthesis, possibly in the form of CaCl2, CaSO4, etc., magnesium salt (such as Mg 2+ ), which is a component of chlorophyll, possibly in the form of MgCl2, MgSO4, etc.
[0042] In some embodiments, the Cl - content in the algal organic solid waste is 100-150mg / L.
[0043] In some embodiments, the preparation method of the inoculated microorganisms is:
[0044] The sludge was placed in an anaerobic fermenter and anaerobic fermented at 35-36℃ for 30-40 days. After settling for 30-40 minutes, the supernatant was removed to obtain the inoculated microorganisms. The sludge contained MLSS (total suspended solids) of 8-16 g / L and MLVSS (volatile suspended solids) of 6.5-14 g / L.
[0045] In some embodiments, the anaerobic fermentation is controlled at a temperature of 33–38°C for 6–16 days.
[0046] In some embodiments, nitrogen gas is introduced into the anaerobic digester to remove oxygen, and the nitrogen gas introduction time is 5 to 10 minutes.
[0047] In some embodiments, the fermentation broth after anaerobic fermentation is centrifuged to separate the solid and liquid phases to obtain a bio-carbon source;
[0048] The centrifugation speed was 3000–8000 r / min, and the centrifugation time was 5–8 min.
[0049] In some embodiments, the fermentation substrate is added to the anaerobic fermenter, followed by the addition of inoculated microorganisms. The pH is adjusted to 7-8 using a 0.5 mol / L NaOH solution to remove oxygen from the anaerobic fermenter, thus initiating anaerobic fermentation.
[0050] In some embodiments, the anode is a titanium plate loaded with a ruthenium-iridium oxide coating, namely Ti / RuO2-IrO2 (i.e., titanium-based ruthenium dioxide-iridium dioxide coated electrode), and the cathode is a titanium plate.
[0051] This invention provides a method for the high-efficiency conversion of algal organic solid waste into a biogenic carbon source. Without the addition of additional chemicals, it directly treats algal organic solid waste using electrochemical methods. This not only improves the biodegradability of algal organic solid waste and further enhances its efficient resource utilization, but also reduces costs and the risk of byproducts generated from the addition of chemicals. Electrochemical methods are characterized by high efficiency, broad applicability, high cleanliness, and environmental friendliness. They can remove algae from water bodies without producing toxic or harmful byproducts, ensuring the safety of the water body and its subsequent natural ecological safety. It also avoids the use of other additional chemicals. Through electrochemical treatment and anaerobic fermentation of actual algae, volatile fatty acids, a biogenic carbon source, are generated more efficiently, significantly improving efficiency compared to traditional sludge fermentation and even compared to fermentation using a single algae-enriched substrate, resulting in a 19% increase in yield.
[0052] The following further illustrates the method for high conversion of algal organic solid waste into a bio-carbon source according to the present application with specific examples. This part further illustrates the content of the present application in combination with specific examples, but should not be understood as a limitation of the present application. If not specifically stated, the technical means adopted in the examples are conventional means familiar to those skilled in the art. Unless specifically stated, the reagents, methods and equipment adopted in the present application are conventional reagents, methods and equipment in the art.
[0053] Example 1
[0054] The present example provides a method for high conversion of algal organic solid waste into a bio-carbon source, comprising the following steps:
[0055] S1, take out the algal sludge (specifically, blue-green algae sludge) obtained from natural lake water, add pure water to prepare an algal organic solid waste inoculum with a solid suspension concentration of 10 g / L, take out 500 mL of the inoculum (pH = 6.9), which is the algal organic solid waste; the Cl - content in the algal organic solid waste is 150 mg / L;
[0056] The algal organic solid waste is subjected to electrochemical pretreatment, specifically comprising:
[0057] The algal organic solid waste is placed in an electrolytic cell, an anode (Ti / RuO2-IrO2) and a cathode (titanium plate) are placed in the electrolytic cell, and the anode and the cathode are electrically connected to the positive and negative poles of a power source, respectively, for electrolysis;
[0058] The electrolysis voltage is controlled to be 2.5 V and the electrolysis time is controlled to be 60 min during electrolysis;
[0059] The algal organic solid waste is subjected to electrochemical pretreatment to obtain a fermentation substrate;
[0060] S2, the sludge is placed in an anaerobic fermentation tank and subjected to anaerobic fermentation at 35°C for 40 d, after settling for 30 min, the supernatant is removed, and the inoculum microorganism is obtained; wherein the MLSS (i.e. total solid suspension concentration) in the sludge is 12 g / L and the MLVSS (i.e. volatile solid suspension) is 8.2 g / L;
[0061] S3, 230 mL of the fermentation substrate in S1 is added to the anaerobic fermentation tank, the inoculum microorganism is further added, the pH is adjusted to 7.0 (using a 0.5 mol / L NaOH solution to adjust the pH to 7), nitrogen gas is introduced into the anaerobic fermentation tank for 5 min to remove oxygen and ensure an anaerobic environment, the anaerobic fermentation tank is sealed, and the anaerobic fermentation is carried out in a constant temperature incubator with the temperature controlled at 36°C;
[0062] S4, the fermentation liquid after anaerobic fermentation is subjected to solid-liquid separation, and the supernatant obtained is the bio-carbon source rich in volatile fatty acids; the centrifugation speed is 8000 r / min and the centrifugation time is 5 min.
[0063] Comparative Example 1
[0064] This embodiment provides a method for the efficient conversion of algal organic solid waste into a biological carbon source, including the following steps:
[0065] S1. Take out the algal sludge (specifically, cyanobacteria sludge) obtained from natural lake water, add pure water to prepare an algal organic solid waste inoculation substrate with a solid suspension concentration of 10g / L, and take out 500mL of the inoculation substrate (pH=6.9), which is the algal organic solid waste; the Cl in the algal organic solid waste - The content is 150 mg / L;
[0066] S2. The sludge was placed in an anaerobic fermenter and anaerobic fermented at 35°C for 40 days. After settling for 30 minutes, the supernatant was removed to obtain the inoculated microorganisms. The MLSS (total suspended solids) in the sludge was 12 g / L, and the MLVSS (volatile suspended solids) was 8.2 g / L.
[0067] S3. Add 230 mL of algal organic solid waste from S1 to the anaerobic fermenter, then add inoculated microorganisms, adjust the pH to 7.0, and purge nitrogen into the anaerobic fermenter for 5 minutes to remove oxygen and ensure an anaerobic environment. Seal the anaerobic fermenter and place it in a constant temperature incubator for anaerobic fermentation, controlling the temperature at 36℃.
[0068] S4. After anaerobic fermentation, the fermentation broth is separated into solid and liquid components. The supernatant obtained is a bio-carbon source rich in volatile fatty acids. The centrifugation speed is 8000 r / min and the centrifugation time is 5 min.
[0069] Following the methods described in Example 1 and Comparative Example 1 above, the yield of volatile fatty acids in the supernatant was as follows under different anaerobic fermentation times (0–16 days): Figure 1 As shown.
[0070] from Figure 1 As can be seen, in Comparative Example 1, the algal organic solid waste was not treated with electrochemical methods, and the yield of volatile fatty acids (including acetic acid, propionic acid, isobutyric acid, n-butyric acid, valeric acid, etc.) in the supernatant was relatively low. The yield of volatile fatty acids reached its highest value at 8 days and then decreased. In contrast, in Example 1, the algal organic solid waste was treated with 2.5V electrochemical methods, and the volatile fatty acid content at 10 days was 5419 mg / L, while in Comparative Example 1 it was 3827 mg / L, representing a 41.7% increase in volatile fatty acid content at 10 days.
[0071] Comparative Example 2
[0072] This comparative example provides a method for producing acid through anaerobic fermentation of algae, including the following steps:
[0073] (1) Preparation of electrolyte:
[0074] Take 5g of blue-green algae powder and dissolve it in 50g of distilled water. Centrifuge at 5000 rpm for 10 minutes. Take the supernatant and dilute it with sterile deionized water to obtain a blue-green algae solution. The concentration of blue-green algae cells in the solution is 5.0 x 10 6 cells / mL. Then, add Na2SO4 as an electrolyte to the blue-green algae solution. The solubility of sodium sulfate is 10 mM. Stir until uniform. Form the electrolyte.
[0075] (2) Electrolytic pretreatment:
[0076] In a 250 mL beaker, place the stainless steel 316 anode plate (50 mm x 80 mm, i.e., 80 mm in length and 50 mm in width) and the stainless steel 316 cathode plate (50 mm x 80 mm) parallel and completely in the 250 mL electrolyte. Supply power from a direct current power source (providing a voltage and current of 0-25 V and 0-8 A). Maintain stirring (150 rpm) throughout the process. Electrolyze at a current density of 10 mA / cm 2 for 15 minutes to obtain the pretreated blue-green algae solution.
[0077] (3) Anaerobic fermentation:
[0078] Inoculate 250 mL of the pretreated blue-green algae solution into 60 mL of acclimated sludge (anaerobic granular sludge) in a 600 mL light-tight Erlenmeyer flask. Purge with N2 for 2 minutes to remove the air above the flask. Seal the flask with a rubber stopper and place it in a constant-temperature water bath shaker. Perform anaerobic fermentation at 35°C and 300 rpm to produce organic acids. Measure the concentration of organic acids in the Erlenmeyer flask after 14 days.
[0079] The blue-green algae powder is obtained by separating the blue-green algae mud from the algae water separation station at the Wuxi Binhu District Lujiaokou blue-green algae salvage site. Drain the water in a 500-mesh filter cloth, then dry at 60°C. Grind and sieve (80 mesh) the dried material to obtain the blue-green algae powder.
[0080] Acclimated sludge: Specifically, anaerobic granular sludge, taken from an IC reactor of a certain citric acid factory in Wuxi City. Before inoculation, the anaerobic granular sludge is subjected to heat pretreatment and activation treatment. The heat pretreatment involves heating the anaerobic granular sludge (121°C, 15 minutes) to kill the methanogens and leave the spores of organic acid-producing bacteria. The activation involves culturing the heat-treated sludge in a glucose solution with a COD of 5000 mg / L for one week to allow the spores of organic acid-producing bacteria to germinate, thereby restoring and enhancing the activity of the sludge to achieve better fermentation results.
[0081] According to the method in Comparative Example 2, the yield of organic acids was 4664 mg / L; while the yield of volatile fatty acids produced in Example 1 was significantly improved, reaching a maximum of 5541 mg / L, which was 19% higher than the yield of volatile fatty acids in Comparative Example 2.
[0082] According to the method in Comparative Example 2, the yield of organic acids was 4664 mg / L; while the yield of volatile fatty acids produced in Example 1 was significantly improved, reaching a maximum of 5541 mg / L, which was 19% higher than the yield of volatile fatty acids in Comparative Example 2.
[0083] It can be understood that any combination of the technical features of the above-described embodiments can be made, and for the sake of brevity, all possible combinations of the technical features in the above-described embodiments are not described, however, as long as the combination of the technical features does not exist, it should be considered as the scope of the description.
[0084] The above is only a preferred embodiment of the present application, only the technical principle of the present application is described, these descriptions are only for explaining the principle of the present application, and cannot be explained as the limitation of the protection scope of the present application in any way. Based on the explanation here, any modification, equivalent replacement and improvement made within the spirit and principle of the present application, and other specific embodiments of the present application which can be thought by those skilled in the art without creative labor, should be included in the protection scope of the present application.
Claims
1. A method for high conversion of algal organic solid waste into a bio-carbon source, characterized by, The method comprises the following steps: The algal organic solid waste is subjected to electrochemical pretreatment to obtain a fermentation substrate; The fermentation substrate is added to an anaerobic fermentation tank, inoculated microorganisms are added, oxygen in the anaerobic fermentation tank is removed, and anaerobic fermentation is performed; The fermentation liquid after anaerobic fermentation is subjected to solid-liquid separation to obtain a biological carbon source; The voltage is controlled to be 2.5-10 V during the electrochemical pretreatment.
2. The method of high conversion of algal organic solid waste into bio-carbon source as claimed in claim 1, wherein, The electrochemical pretreatment of the algal organic solid waste specifically comprises: The algal organic solid waste is placed in an electrolytic tank, an anode and a cathode are placed in the electrolytic tank, the anode and the cathode are electrically connected to the positive electrode and the negative electrode of a power supply, respectively, and electrolysis is performed; The electrolysis voltage is controlled to be 2.5-10 V, and the electrolysis time is controlled to be 30-120 min.
3. The method of claim 1, wherein the algal organic solid waste is converted into a bio-carbon source at a high rate. The concentration of solid suspensions in the algal organic solid waste is 10-200 g / L.
4. The method of claim 1, wherein the algal organic solid waste is converted into a bio-carbon source at a high rate. The content of Cl in the algal organic solid waste is 100-150 mg / L. - 100-150 mg / L.
5. The method of high conversion of algal organic solid waste into bio-carbon source as claimed in claim 1 wherein, The preparation method of the inoculated microorganisms is as follows: The sludge is placed in an anaerobic fermentation tank and subjected to anaerobic fermentation at 35-36 ℃ for 30-40 d, and after sedimentation, the supernatant is removed to obtain the inoculated microorganisms; the MLSS in the sludge is 8-16 g / L, and the MLVSS is 6.5-14 g / L.
6. The method of high conversion of algal organic solid waste into bio-carbon source as claimed in claim 1 wherein, The temperature is controlled to be 33-38 ℃, and the time is controlled to be 6-16 d during the anaerobic fermentation.
7. The method of high conversion of algal organic solid waste into bio-carbon source as claimed in claim 1 wherein, Oxygen in the anaerobic fermentation tank is removed by introducing nitrogen into the anaerobic fermentation tank, and the nitrogen introduction time is 5-10 min.
8. The method of high conversion of algal organic solid waste into bio-carbon source as claimed in claim 1 wherein, The fermentation liquid after anaerobic fermentation is subjected to centrifugal solid-liquid separation to obtain a biological carbon source; The centrifugal speed is 3000-8000 r / min, and the centrifugal time is 5-8 min.
9. The method of high conversion of algal organic solid waste into bio-carbon source as claimed in claim 1 wherein, The fermentation substrate is added to an anaerobic fermentation tank, inoculated microorganisms are added, the pH is adjusted to 7-8, oxygen in the anaerobic fermentation tank is removed, and anaerobic fermentation is performed.
10. The method of high conversion of algal organic solid waste into bio-carbon source as claimed in claim 2 wherein, The anode is a titanium plate loaded with a ruthenium-iridium oxide coating, and the cathode is a titanium plate.