Preparation method of raw material with high yield, high moisture retention and high antioxidant activity

Abstract

The invention discloses a preparation method of a high-yield, high-moisture-retention and high-antioxidant-activity raw material, which comprises the following steps: 1) preparing a fermentation culture medium: preparing the fermentation culture medium by using 8-12% by mass of edible sugar, 0.3-1% by mass of soybean meal and the balance of deionized water as raw materials; (2) deep liquid fermentation: sterilizing a fermentation culture medium, and sterilely inoculating the prepared schizophyllum commune seed solution to obtain schizophyllum commune polysaccharide fermentation liquor; and 3) polysaccharide extraction: homogenizing the schizophyllum commune polysaccharide fermentation liquor, and extracting schizophyllum commune crude polysaccharide by adopting a traditional alcohol extraction method. Wild schizophyllum commune is utilized, a full-food-grade raw material characterized by soybean meal is selected to prepare a fermentation culture medium, the schizophyllum commune polysaccharide is prepared through high-yield fermentation, and the schizophyllum commune polysaccharide has high moisturizing and high antioxidant activity and is a potential moisturizing and antioxidant skin care product raw material. Compared with traditional schizophyllum commune fermentation, the method has the advantages of being high in yield, high in activity, easy to operate and the like, and has remarkable economic benefits.

Description

Technical Field

[0001] This invention relates to the fields of fermentation engineering and cosmetics, and in particular to a method for preparing a high-yield, highly moisturizing, and highly antioxidant active ingredient. Background Technology

[0002] Skincare products are daily care products used to protect and improve skin condition, encompassing functions such as cleansing, moisturizing, anti-aging, nourishing, and sun protection. Common forms include facial cleansers, toners, lotions, and creams. Their function is to maintain skin's moisture balance, replenish nutrients, and protect against external aggressors. Moisturizing, as a core function of cosmetics, has always received significant attention. In cosmetic formulation design, selecting suitable moisturizers is crucial for maintaining skin hydration, alleviating dryness, and facilitating the absorption of active ingredients. Furthermore, one of the main causes of skin aging is the generation of free radicals by external environmental stimuli, leading to oxidative stress and accelerating cell damage. Efficiently absorbing and locking in moisture, as well as eliminating free radicals, are key to achieving moisturizing and anti-aging functions in skincare products. Currently, the main moisturizing ingredients in skincare products include hyaluronic acid, glycerin, alginic acid, and squalane; the main anti-aging ingredients include vitamin C, vitamin E, tea polyphenols, and coenzyme Q10. Because each ingredient has a single function, to achieve multiple effects in skincare products, they need to be combined or used in sets, which creates inconvenience for both production and consumption.

[0003] Schizophyllum polysaccharides are the main components of Schizophyllum commune, primarily composed of β-glucan with β(1→3) and β(1→6) glycosidic bonds. They exhibit remarkable efficacy in immunomodulation, antitumor activity, and antioxidation, and are widely used in food, medicine, and many other fields. Studies have found that Schizophyllum polysaccharides also possess good moisturizing properties. Combined with their antioxidant activity, their application in skincare products could produce a multi-functional effect. Schizophyllum polysaccharides can be extracted from Schizophyllum fruiting bodies or prepared using submerged liquid fermentation. The production of Schizophyllum polysaccharides is limited by artificial cultivation techniques and a long production cycle. Submerged liquid fermentation can conveniently produce Schizophyllum polysaccharides on a large scale, but current yields are generally low, with the highest international yield being only 7.9 g / L, resulting in high production costs. Furthermore, the culture media currently used for Schizophyllum polysaccharide fermentation are generally complex in composition and expensive, further increasing production costs and making the process difficult. Therefore, the fermentation technology of Schizophyllum commune polysaccharides, which is simple to operate, has high yield, high moisture retention and high antioxidant activity, is particularly important. Summary of the Invention

[0004] Objective of the Invention: To address the problems existing in the prior art, this invention provides a method for preparing a high-yield, high-moisturizing, and high-antioxidant-activity raw material. This method abandons the complex formulation of conventional Schizophyllum commune fermentation media, using only edible sugar and soybean flour as raw materials for deep liquid fermentation to produce Schizophyllum commune polysaccharides. This simplifies the Schizophyllum commune polysaccharide production process, makes it easy to operate, and achieves high yield and excellent moisturizing and antioxidant functions for Schizophyllum commune polysaccharides.

[0005] Technical solution: This invention provides a method for preparing a high-yield, highly moisturizing, and highly antioxidant active raw material, comprising the following steps:

[0006] 1) Preparation of fermentation medium: The raw materials used are only edible sugar and soybean flour, and the mass ratio (the same below) is 8-12% edible sugar, 0.3-1% soybean flour, and the remainder is deionized water to prepare the fermentation medium;

[0007] 2) Submerged liquid fermentation: After sterilizing the fermentation medium, aseptically inoculate the prepared Schizophyllum commune seed liquid at a volume ratio of 4-8%, control the rotation speed at 100-300 r / min, the ventilation rate at 1:0.6-1:1.2 and the temperature at 25-32℃, and ferment for 4-7 days to obtain Schizophyllum commune polysaccharide fermentation broth;

[0008] 3) Polysaccharide extraction: Homogenize the fermentation broth of Schizophyllum commune polysaccharide and extract crude polysaccharide from Schizophyllum commune using the traditional alcohol extraction method.

[0009] Even better, in step 1), the fermentation medium is prepared with 11% sugar, 0.7% soybean flour, and the remainder is deionized water.

[0010] Preferably, in step 2), the selected Schizophyllum commune was screened from the Shennongjia primeval forest and has not been biomodified.

[0011] Preferably, *Schizophyllum commune* mycelium is isolated by plate culture and preserved in test tubes. A potato-glucose aqueous solution is prepared, and 100 mL of this solution is added to a 250 mL Erlenmeyer flask. After routine sterilization, 2-3 *Schizophyllum commune* preservation slant plates are inoculated. The mixture is then shaken at 150 rpm and cultured at 28 °C for 3-5 days. Finally, the solution is transferred at a 10% volume ratio to a 500 mL Erlenmeyer flask containing 200 mL of potato-glucose aqueous solution and cultured for another 4 days to obtain *Schizophyllum commune* seed culture for later use.

[0012] Even better, after sterilizing the fermentation medium, the prepared *Schizophyllum commune* seed culture was aseptically inoculated at a volume ratio of 5%. The fermentation speed was controlled at 100-300 rpm and the aeration rate at a ratio of 1:0.6-1:1.2, depending on the state of the fermentation broth. Fermentation was carried out at 28°C for 5 days to obtain *Schizophyllum commune* polysaccharide fermentation broth. The yield of *Schizophyllum commune* polysaccharide in the fermentation broth reached 16.8 g / L.

[0013] Preferably, the prepared Schizophyllum commune crude polysaccharide is used as a raw material for moisturizing and antioxidant skin care products.

[0014] Beneficial effects:

[0015] 1) This invention selects wild-type Schizophyllum commune instead of modified strains in order to preserve the natural properties of the production strain as much as possible. In addition, wild-type strains have stronger resistance to contamination and are easier to operate compared to modified strains.

[0016] 2) The fermentation medium uses only edible sugar and soybean flour, without adding any other chemical substances, which reduces the chemical residues in the extract of Schizophyllum commune polysaccharide, making it safer to use as a raw material in skin care products.

[0017] 3) Using insoluble soybean flour allows for the slow release of nitrogen and carbon during fermentation, thus preventing nutrient deficiency in the fermentation broth during the later stages of fermentation and ensuring the continuous and rapid synthesis of Schizophyllum commune polysaccharides.

[0018] 4) Soybean powder is degraded and utilized by Schizophyllum commune during fermentation, which eliminates allergens in soybean powder and ensures the safety of subsequent skin care products.

[0019] 5) By controlling the fermentation conditions using this culture medium, the yield of *Schizophyllum commune* polysaccharide was significantly increased, more than doubling the highest international fermentation level. The prepared *Schizophyllum commune* polysaccharide exhibited superior moisture retention compared to traditional sodium alginate and polyethylene glycol, and also possessed better DPPH and hydroxyl radical scavenging capabilities (IC50). 50 (The value is smaller).

[0020] In summary, this invention utilizes wild-type Schizophyllum commune and optimizes a fermentation medium containing only two edible natural components (edible sugar and soybean flour). Under optimized fermentation conditions, high-yield production of Schizophyllum commune polysaccharides is achieved. The prepared Schizophyllum commune polysaccharides exhibit high moisture retention and high antioxidant activity, and are also human-friendly. Therefore, compared to traditional Schizophyllum commune fermentation, this invention offers advantages such as high yield, high activity, and ease of operation, resulting in significant economic benefits. Attached Figure Description

[0021] Figure 1 The moisture absorption rate of Schizophyllum commune polysaccharide at 43% relative humidity;

[0022] Figure 2 The moisture absorption rate of Schizophyllum commune polysaccharide at 81% relative humidity;

[0023] Figure 3 The moisture retention rate of Schizophyllum commune polysaccharide at 34% relative humidity;

[0024] Figure 4 It is the scavenging rate of DPPH free radicals by Schizophyllum commune polysaccharide;

[0025] Figure 5Schizophyllum polysaccharide scavenging rate against hydroxyl radicals. Detailed Implementation

[0026] The present invention will now be described in detail with reference to specific embodiments.

[0027] Example 1: Preparation of Wild-type Schizophyllum commune seed liquid

[0028] A strain of *Schizophyllum commune* was collected from the Shennongjia primeval forest. The mycelium was isolated by plate culture and preserved in test tubes. A potato-glucose aqueous solution was prepared. 100 mL of this solution was added to a 250 mL Erlenmeyer flask, sterilized according to standard procedures, and inoculated onto 2-3 *Schizophyllum commune* preservation slant agar plates. The mixture was incubated at 28°C for 3-5 days using a shaker at 150 rpm. Then, at a 10% (v / v) ratio, it was transferred to a 500 mL Erlenmeyer flask containing 200 mL of potato-glucose aqueous solution and incubated for another 4 days to obtain the *Schizophyllum commune* seed culture for later use.

[0029] Example 2: Preparation of Schizophyllum commune polysaccharide by deep liquid fermentation

[0030] In a 5L mechanically stirred tank, 3L of deionized water was added, followed by 330g of edible sugar and 21g of soybean powder. After stirring evenly, the mixture was sterilized at 115℃ for 25 minutes. After cooling to 28℃, 150mL of the seed culture prepared in Example 1 was aseptically inoculated. The fermentation speed (100-300 r / min) and aeration rate (1:0.6-1:1.2) were controlled according to the fermentation broth's state, and fermentation was carried out at 28℃ for 5 days. The fermentation broth was homogenized using a high-speed homogenizer and then transferred to 12L of anhydrous ethanol. After stirring, the mixture was allowed to settle for 12 hours. The solid was obtained by centrifugation. The obtained solid was then added to 3L of deionized water and dissolved completely. After centrifugation, the liquid fraction was obtained. 5mL of the liquid fraction was tested using the sulfuric acid-phenol method to determine the crude polysaccharide content, which was 16.8g / L. The liquid fraction was then transferred to 12L of anhydrous ethanol, stirred, and allowed to settle for 12 hours. After centrifugation, the solid fraction was obtained. The solid fraction was freeze-dried to yield 46.3g of *Schizophyllum commune* crude polysaccharide powder.

[0031] Example 3: Preparation of Schizophyllum commune polysaccharide by deep liquid fermentation

[0032] In a 5L mechanically stirred tank, 3L of deionized water was added, followed by 240g of edible sugar and 9g of soybean powder. After stirring evenly, the mixture was sterilized at 115℃ for 25 minutes. After cooling to 25℃, 120mL of the seed culture prepared in Example 1 was aseptically inoculated. The fermentation speed (100-300 r / min) and aeration rate (1:0.6-1:1.2) were controlled according to the fermentation broth's state, and fermentation was carried out at 25℃ for 4 days. The fermentation broth was homogenized using a high-speed homogenizer and then transferred to 12L of anhydrous ethanol. After stirring, the mixture was allowed to settle for 12 hours. The solid was obtained by centrifugation. The obtained solid was then added to 3L of deionized water and dissolved completely. After centrifugation, the liquid fraction was obtained. 5mL of the liquid fraction was tested using the sulfuric acid-phenol method to determine the crude polysaccharide content, which was 11.7g / L. The liquid fraction was then transferred to 12L of anhydrous ethanol, stirred, and allowed to settle for 12 hours. After centrifugation, the solid fraction was obtained. The solid fraction was freeze-dried to obtain 32.1g of *Schizophyllum commune* crude polysaccharide powder.

[0033] Example 4: Preparation of Schizophyllum commune polysaccharide by deep liquid fermentation

[0034] In a 5L mechanically stirred tank, 3L of deionized water was added, followed by 360g of edible sugar and 30g of soybean powder. After stirring evenly, the mixture was sterilized at 115℃ for 25 minutes. After cooling to 32℃, 240mL of the seed culture prepared in Example 1 was aseptically inoculated. The fermentation speed (100-300 r / min) and aeration rate (1:0.6-1:1.2) were controlled according to the fermentation broth's state, and fermentation was carried out at 32℃ for 7 days. The fermentation broth was homogenized using a high-speed homogenizer and then transferred to 12L of anhydrous ethanol. After stirring, the mixture was allowed to settle for 12 hours. The solid was obtained by centrifugation. The obtained solid was then added to 3L of deionized water and dissolved completely. After centrifugation, the liquid fraction was obtained. 5mL of the liquid fraction was tested using the sulfuric acid-phenol method to determine the crude polysaccharide content, which was 16.3g / L. The liquid fraction was then transferred to 12L of anhydrous ethanol, stirred, and allowed to settle for 12 hours. After centrifugation, the solid fraction was obtained. The solid fraction was freeze-dried to yield 45.2g of *Schizophyllum commune* crude polysaccharide powder.

[0035] Example 5: Determination of the moisturizing and antioxidant activities of Schizophyllum commune polysaccharides

[0036] I. Method for determining the moisture retention of polysaccharides from Schizophyllum commune

[0037] (I) Method for determining hygroscopicity

[0038] Accurately weigh 0.5g of the Schizophyllum commune polysaccharide prepared in Example 2, and accurately weigh 0.5g of polyethylene glycol and 0.5g of sodium alginate as controls. Under room temperature conditions, place these three reagents into two desiccators with relative humidity of 43% and 81%, respectively. First, weigh the mass when the reagents are placed in the desiccator, and then continuously weigh and record the mass at 1h, 2h, 3h, 5h, 8h, 12h, 24h, 48h, 60h, 72h, 84h, and 101h. Calculate the moisture retention using the following formula.

[0039] Moisture absorption rate = (m x -m0) / m0×100%

[0040] In the formula: m0 is the mass (g) of the first weighing, m x The mass (g) measured at different time periods.

[0041] (II) Methods for Measuring Moisturizing Properties

[0042] Accurately weigh 0.5 g of the Schizophyllum commune polysaccharide prepared in Example 2, and accurately weigh 0.5 g of polyethylene glycol and 0.5 g of sodium alginate as controls. Add 0.5 mL of ultrapure water to each of the three reagents. Then, place them in a desiccator with a relative humidity of 34% at room temperature. First, weigh the mass (including reagents and ultrapure water) when the desiccator is placed in the desiccator. Then, continuously weigh the mass at 1 h, 2 h, 3 h, 5 h, 8 h, 12 h, 24 h, 48 h, 60 h, 72 h, 84 h, and 101 h, and record the mass. Calculate the hygroscopicity according to the following formula.

[0043] Moisture retention rate = m x / m0×100%

[0044] In the formula: m0 is the mass (g) of the first weighing, m x The mass (g) measured at different time periods.

[0045] II. Method for determining the antioxidant activity of Schizophyllum commune polysaccharides

[0046] (I) Method for determining the scavenging rate of DPPH free radicals

[0047] Prepare *Schizophyllum commune* polysaccharide solutions (0.5 mg / mL, 1 mg / mL, 1.5 mg / mL, 3 mg / mL, 3.5 mg / mL, 4 mg / mL, 4.5 mg / mL, 5 mg / mL, 6 mg / mL) as described in Example 2. Take 1.5 mL of each concentration of *Schizophyllum commune* polysaccharide solution into a test tube, add 0.5 mL of ultrapure water and 2 mL of 0.2 mmol / L DPPH solution, shake well, and let stand at room temperature for 30 minutes in the dark. Simultaneously, mix 2 mL of each concentration of *Schizophyllum commune* polysaccharide solution with 2 mL of anhydrous ethanol, shake well, and let stand at room temperature for 30 minutes. Measure the absorbance at 517 nm, and record them as A1 and A2, respectively. Use 2 mL of anhydrous ethanol with 2 mL of DPPH solution as a blank, and measure the absorbance value as A0. The clearance rate is calculated using the following formula:

[0048] DPPH free radical scavenging rate = [1 - (A1 - A2) / A0] × 100%

[0049] (II) Method for determining the scavenging rate of hydroxyl radicals

[0050] Prepare *Schizophyllum commune* polysaccharide solutions (0.5 mg / ml, 1 mg / ml, 1.5 mg / ml, 2 mg / ml, 2.5 mg / ml, 3 mg / ml) as described in Example 2. Take 1 mL of each concentration of *Schizophyllum commune* polysaccharide solution into a test tube, then add 1 mL of 2 mmol / L ferrous sulfate solution, 1 mL of 6 mmol / L salicylic acid solution, and 1 mL of 6 mmol / L hydrogen peroxide solution. Replace the hydrogen peroxide solution with distilled water, keeping other conditions unchanged. After shaking and mixing, incubate in the dark at room temperature. After 30 minutes, measure the absorbance at 510 nm, which are A1 and A2, respectively. Add 1 mL of distilled water to 1 mL of 2 mmol / L ferrous sulfate solution, 1 mL of 6 mmol / L salicylic acid solution, and 1 mL of 6 mmol / L hydrogen peroxide solution, and measure the absorbance value as A0. The scavenging rate is calculated using the following formula:

[0051] Hydroxyl radical scavenging rate = [1 - (A1 - A2) / A0] × 100%

[0052] III. Measurement Results

[0053] The moisturizing effect of Schizophyllum commune polysaccharide is as follows Figure 1-3 As shown, by Figure 1-3 It can be seen that the Schizophyllum polysaccharide prepared in Example 2 has better moisturizing properties than polyethylene glycol and sodium alginate, and is an excellent raw material for moisturizing skin care products.

[0054] Antioxidant results of Schizophyllum commune polysaccharide are as follows Figure 4-5 As shown, calculations revealed that the IC50 of the *Schizophyllum commune* polysaccharide prepared in Example 2 against DPPH and hydroxyl radicals... 50 The concentrations were 1.06 mg / mL and 1.85 mg / mL, respectively, which are superior to the antioxidant activity of Schizophyllum commune polysaccharide reported to date, making it an excellent raw material for antioxidant skin care products.

[0055] Comparative Example 1: Production of Schizophyllum polysaccharides by fermentation using domesticated (modified) Schizophyllum.

[0056] Domesticated (modified) *Schizophyllum commune* was used to replace the wild *Schizophyllum commune* in Example 1 for cultivation to prepare domesticated (modified) *Schizophyllum commune* seed culture. In a 5L mechanically stirred tank, 3L of deionized water was added, followed by 330g of edible sugar and 21g of soybean powder. After stirring evenly, the mixture was sterilized at 115℃ for 25 minutes. After cooling to 28℃, 150mL of the domesticated *Schizophyllum commune* seed culture was aseptically inoculated. The fermentation speed (100-300 rpm) and aeration rate (1:0.6-1:1.2) were controlled according to the fermentation broth state, and fermentation was carried out at 28℃ for 5 days. The fermentation broth was homogenized using a high-speed homogenizer. 10mL of the homogenized fermentation broth was transferred to 40mL of anhydrous ethanol, stirred thoroughly, and allowed to stand for 12 hours to settle. The solid was obtained by centrifugation. The obtained solid was then dissolved in 10mL of deionized water and centrifuged to obtain the liquid fraction. 5mL of the liquid fraction was taken and the crude polysaccharide content was determined by the sulfuric acid-phenol method to be 6.87g / L. The difference between Example 2 and Example 3 lies in the strain. Example 2 used screened wild Schizophyllum commune, while Comparative Example 1 used domesticated (modified) Schizophyllum commune. Due to the difference in strains, the degree of utilization of soybean flour in the fermentation medium differs, which in turn leads to the difference in the yield of Schizophyllum commune polysaccharides.

[0057] Comparative Example 2: Preparation of Schizophyllum commune polysaccharide using traditional fermentation media

[0058] First, add 3L of deionized water to a 5L mechanically stirred tank, then add 150g of glucose, 15g of yeast extract, 3g of KH2PO4, 1.5g of MgSO4·7H2O, 0.3g of oleic acid, and 0.015g of citric acid. After stirring evenly, adjust the pH to about 6.0, sterilize at 121℃ for 20 minutes, cool to 28℃, and aseptically inoculate 150mL of the Schizophyllum commune seed culture prepared in Example 1. Control the fermentation speed (100-300r / min) and aeration rate (1:0.6-1:1.2) according to the state of the fermentation broth, and ferment at 28℃ for 5 days. The fermentation broth was homogenized using a high-speed homogenizer. 10 mL of the homogenized broth was transferred to 40 mL of anhydrous ethanol, stirred thoroughly, and allowed to settle for 12 hours. The solid was then centrifuged. The solid was dissolved in 10 mL of deionized water and centrifuged again to obtain the liquid fraction. 5 mL of the liquid fraction was analyzed using the sulfuric acid-phenol method to determine the crude polysaccharide content, which was 5.37 g / L. The difference between Comparative Example 2 and Example 2 lies in the formulation of the fermentation medium. Comparative Example 2 used a traditional fermentation medium, whose components are readily soluble in deionized water and rapidly metabolized by *Schizophyllum commune*. This resulted in a higher yield of *Schizophyllum commune* polysaccharide in the early stages of fermentation, but insufficient yield in the later stages, leading to a significant difference in final yield compared to Example 2.

[0059] The above embodiments are only for illustrating the technical concept and features of the present invention, and are intended to enable those skilled in the art to understand the content of the present invention and implement it accordingly. They should not be construed as limiting the scope of protection of the present invention. All equivalent transformations or modifications made in accordance with the spirit and essence of the present invention should be covered within the scope of protection of the present invention.

Claims

1. A method for preparing a high-yield, highly moisturizing, and highly antioxidant active raw material, characterized in that: Includes the following steps: 1) Preparation of fermentation medium: The raw materials used are only edible sugar and soybean flour, with a mass ratio of 8-12% edible sugar, 0.3-1% soybean flour, and the remainder being deionized water to prepare the fermentation medium; 2) Submerged liquid fermentation: After sterilizing the fermentation medium, aseptically inoculate the prepared Schizophyllum commune seed liquid at a volume ratio of 4-8%, control the rotation speed at 100-300 r / min, the ventilation rate at 1:0.6-1:1.2 and the temperature at 25-32℃, and ferment for 4-7 days to obtain Schizophyllum commune polysaccharide fermentation broth; 3) Polysaccharide extraction: Homogenize the fermentation broth of Schizophyllum commune polysaccharide and extract crude polysaccharide from Schizophyllum commune using the traditional alcohol extraction method.

2. The method for preparing high-yield, high-moisturizing, and high-antioxidant-activity raw materials according to claim 1, characterized in that: In step 1), the fermentation medium is prepared with 11% edible sugar, 0.7% soybean flour, and the remainder is deionized water.

3. The method for preparing high-yield, high-moisturizing, and high-antioxidant-activity raw materials according to claim 1, characterized in that: In step 2), the selected Schizophyllum commune was screened from the Shennongjia primeval forest and was not biomodified.

4. The method for preparing high-yield, high-moisturizing, and high-antioxidant-activity raw materials according to claim 3, characterized in that: Schizophyllum mycelium was isolated by plate culture and preserved in test tubes. A potato-glucose aqueous solution was prepared, and 100 mL of this solution was added to a 250 mL Erlenmeyer flask. After routine sterilization, 2-3 slants of Schizophyllum culture medium were inoculated into the solution. The mixture was then shaken at 150 rpm and incubated at 28 °C for 3-5 days. Finally, the solution was transferred at a volume ratio of 10% to a 500 mL Erlenmeyer flask containing 200 mL of potato-glucose solution and cultured for another 4 days to obtain the Schizophyllum seed culture for later use.

5. The method for preparing a high-yield, high-moisturizing, and high-antioxidant-activity raw material according to claim 1, characterized in that: After sterilizing the fermentation medium, the prepared Schizophyllum commune seed liquid was aseptically inoculated at a volume ratio of 5%. The fermentation speed was controlled at 100-300 r / min and the ventilation rate was 1:0.6-1:1.2 according to the state of the fermentation liquid. Fermentation was carried out at 28℃ for 5 days to obtain Schizophyllum commune polysaccharide fermentation broth.

6. The method for preparing a high-yield, high-moisturizing, and high-antioxidant-activity raw material according to claim 1, characterized in that: The prepared Schizophyllum commune crude polysaccharide is used as a raw material for moisturizing and antioxidant skin care products.

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