Oligosaccharide selenium ganoderma lucidum capsule capable of enhancing immunity and inhibiting tumors and preparation method of oligosaccharide selenium ganoderma lucidum capsule

Abstract

The invention belongs to the technical field of health-care products, and discloses an oligosaccharide selenium ganoderma lucidum capsule for enhancing immunity and inhibiting tumors and a preparation method, and the content of the capsule is prepared from the following raw materials in parts by mass: 150-160 parts of collagen, 80-85 parts of chitosan oligosaccharide, 110-130 parts of ganoderma lucidum extract, 45-55 parts of selenium-enriched yeast, 50-80 parts of maltodextrin and 7-10 parts of magnesium stearate. The skin barrier can be improved, intercellular communication can be promoted and local immune response can be stimulated through low-molecular peptide of collagen, intestinal flora balance can be adjusted through chitosan oligosaccharide, probiotic growth can be promoted, mucous membrane immunity can be further improved, and finally immune cells can be effectively activated through polysaccharide and triterpenoids in the ganoderma lucidum extract, so that the immunity of a human body is improved. The activity of macrophages and natural killer cells is enhanced, and the multi-target and multi-path immune regulation effect can effectively enhance the defense of the body to pathogenic microorganisms.

Description

Technical Field

[0001] This invention belongs to the field of health product technology, specifically an oligosaccharide selenium Ganoderma lucidum capsule that enhances immunity and inhibits tumors, and its preparation method. Background Technology

[0002] Health supplement capsules refer to solid dosage forms made by scientifically combining and meticulously preparing natural or extracted functional ingredients using capsule technology. They are easy to take, not only for dosage control and oral absorption, but also for protecting the active ingredients from external environmental influences. Health supplement capsules are commonly used to improve human health, regulate immune function, combat aging, or assist in disease prevention and treatment. They are a widely used dosage form in the modern field of nutrition and health care.

[0003] Currently, although various health products are emerging in the market, existing products have some limitations in terms of functional positioning and ingredient combination. First, most products have a high content of a single ingredient and lack a comprehensive regulatory effect of multiple targets and mechanisms, making it difficult to meet the needs of modern people for comprehensive health support. Second, the bioavailability of active ingredients in some health products is low, resulting in a significant reduction in actual effects. Finally, due to changes in modern lifestyles and environmental factors, the human body faces problems such as decreased immune function, chronic inflammation, and increased risk of tumors. Current health products often lack systematic formulation design in terms of improving immunity and anti-tumor effects, and cannot simultaneously regulate the body's immune system, protect cells from oxidative damage, and exert a direct inhibitory effect on tumor cells. Therefore, there is a need for oligosaccharide selenium Ganoderma lucidum capsules that can strengthen immunity and inhibit tumors to meet consumers' needs for overall health regulation. Summary of the Invention

[0004] The purpose of this invention is to provide an oligosaccharide selenium Ganoderma lucidum capsule that enhances immunity and inhibits tumors, as well as a preparation method thereof, in order to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, the present invention provides the following technical solution: an oligosaccharide selenium Ganoderma lucidum capsule for enhancing immunity and inhibiting tumors, and a preparation method thereof, wherein the oligosaccharide selenium Ganoderma lucidum capsule is composed of a capsule shell and capsule contents, wherein the capsule contents are made from the following raw materials in parts by weight: 150-160 parts of collagen, 80-85 parts of chitosan oligosaccharide, 110-130 parts of Ganoderma lucidum extract, 45-55 parts of selenium-enriched yeast, 50-80 parts of maltodextrin, and 7-10 parts of magnesium stearate.

[0006] Preferably, the capsule shell is composed of the following raw materials in parts by weight: 80-100 parts gelatin, 50-65 parts glycerin and 20-80 parts water.

[0007] Preferably, the specific steps of this method are as follows: Step 1, Raw material production and screening: Produce raw material powders of collagen, chitosan oligosaccharide and Ganoderma lucidum extract respectively, and screen each raw material powder to remove impurities and particles that do not meet the particle size requirements to ensure the quality of raw materials; Step 2, Uniform Mixing: According to the design formula of each capsule, accurately weigh the screened raw material powders and mix them thoroughly using a mixer to ensure that the components are evenly distributed. Step 3, Capsule filling: Prepare capsule shells and use a capsule filling machine to precisely fill the capsules with the uniformly mixed powder according to the predetermined dosage to complete the capsule filling process; Step 4, Sealing and Hardening: Place the filled glue in a suitable environment for hardening treatment, so that the capsule shell is fully sealed, and the oligosaccharide selenium Ganoderma lucidum capsule is obtained. Step 5, Packaging: Pack the capsules containing powder to protect the quality of the capsules and facilitate storage and transportation.

[0008] Preferably, the collagen extraction process is as follows: A1. Select fresh, uncontaminated cod skin, thaw it, wash it thoroughly to remove blood and impurities, and then mince it mechanically; A2, steam at 100℃ for 30 minutes, then add 1% pepsin, according to the ratio of raw materials to water. Adjust the pH to 2-3 at a ratio of 1:10, and react at around 37°C for 6 hours to fully hydrolyze the collagen into low molecular weight peptides. A3. Adjust the pH of the solution in A2 to 6-7 to inactivate enzyme activity, and then add diatomaceous earth for decolorization and deodorization treatment; A4, after being filtered to remove suspended solids, is concentrated under reduced pressure at 0.08-0.1 MPa and 50-60℃. A5 involves sterilizing the concentrated solution at 110-122℃ for 15 seconds, and then obtaining powder through a spray drying mechanism.

[0009] Preferably, the chitosan oligosaccharide is prepared by enzymatic hydrolysis of chitosan, and after enzymatic hydrolysis, it is prepared into a dry powder by filtration, concentration and spray drying; In the enzymatic hydrolysis stage, a compound enzyme is required, and the reaction is carried out for 5 hours at a temperature of 50℃ and a pH of 5.0-5.5.

[0010] Preferably, the extraction process of the Ganoderma lucidum extract is as follows: B1. Ganoderma lucidum raw material is crushed and then mixed with eight times the amount of water. B2 was extracted twice at 100℃, with the first extraction lasting 3 hours and the second extraction lasting 2 hours, to ensure that the active ingredients were fully dissolved. B3, the extract was filtered to remove solid impurities, and then the solution was concentrated under reduced pressure; B4. Add 75% ethanol to the concentrate to allow polysaccharides and other impurities to settle, and let stand for 14 hours. B5 was separated and precipitated by vacuum filtration, then dried at 55℃ and -0.05MPa to obtain a stable powder. Finally, it was pulverized and sieved to ensure uniform particle size.

[0011] The beneficial effects of this invention are as follows: 1. This invention utilizes low-molecular-weight peptides of collagen to improve the skin barrier and promote intercellular communication, stimulating local immune responses. Furthermore, chitosan oligosaccharides can regulate the balance of intestinal flora, promote the growth of probiotics, and thus enhance mucosal immunity. Finally, the polysaccharides and triterpenoids in Ganoderma lucidum extract effectively activate immune cells and enhance the activity of macrophages and natural killer cells. This multi-target, multi-pathway immunomodulatory effect can effectively enhance the body's defense against pathogenic microorganisms and lay a solid foundation for anti-tumor effects, significantly improving the body's immune response.

[0012] 2. The curcumin in this invention can reduce the secretion of inflammatory factors by inhibiting inflammatory signaling pathways such as NF-κB and induce tumor cell apoptosis, while blocking cell proliferation signals. Through the rational combination of selenium-enriched yeast, Ganoderma lucidum, collagen, chitosan oligosaccharide, and other extracts, a synergistic effect of multiple health benefits is achieved. This multi-component, multi-mechanism synergistic effect can comprehensively enhance the body's immunity and also has certain anti-tumor potential, effectively improving the health benefits of this oligosaccharide selenium Ganoderma lucidum capsule. Attached Figure Description

[0013] Figure 1 This is a flowchart illustrating the preparation process of the oligosaccharide selenium Ganoderma lucidum capsules of the present invention. Detailed Implementation

[0014] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0015] like Figure 1As shown, this invention provides an oligosaccharide selenium Ganoderma lucidum capsule for enhancing immunity and inhibiting tumors, and a preparation method thereof. The oligosaccharide selenium Ganoderma lucidum capsule consists of a capsule shell and capsule contents, wherein the capsule contents are made from the following raw materials in parts by weight: 150-160 parts collagen, 80-85 parts chitosan oligosaccharide, 110-130 parts Ganoderma lucidum extract, 45-55 parts selenium-enriched yeast, 50-80 parts maltodextrin, and 7-10 parts magnesium stearate.

[0016] Selenium-enriched yeast: should comply with the provisions of GB1903.21 "National Food Safety Standard for Food Fortification Agents: Selenium-enriched Yeast"; Maltodextrin: should comply with the provisions of the "Pharmacopoeia of the People's Republic of China"; Magnesium stearate: should comply with the provisions of the "Pharmacopoeia of the People's Republic of China".

[0017] Low-molecular-weight peptides from collagen can improve the skin barrier and promote intercellular communication, stimulating local immune responses. Chitosan oligosaccharides can regulate the balance of intestinal flora, promote the growth of probiotics, and thus enhance mucosal immunity. Finally, polysaccharides and triterpenoids in Ganoderma lucidum extract can effectively activate immune cells and enhance the activity of macrophages and natural killer cells. This multi-target, multi-pathway immunomodulatory effect can effectively enhance the body's defense against pathogenic microorganisms and lay a solid foundation for anti-tumor effects, significantly improving the body's immune response.

[0018] The capsule shell is composed of the following raw materials in parts by weight: 80-100 parts gelatin, 50-65 parts glycerin and 20-80 parts water.

[0019] The specific steps of this method are as follows: Step 1, Raw material production and screening: Produce raw material powders of collagen, chitosan oligosaccharide and Ganoderma lucidum extract respectively, and screen each raw material powder to remove impurities and particles that do not meet the particle size requirements to ensure the quality of raw materials; Step 2, Uniform Mixing: According to the design formula of each capsule, accurately weigh the screened raw material powders and mix them thoroughly using a mixer to ensure that the components are evenly distributed. Step 3, Capsule filling: Prepare capsule shells and use a capsule filling machine to precisely fill the capsules with the uniformly mixed powder according to the predetermined dosage to complete the capsule filling process; Step 4, Sealing and Hardening: Place the filled glue in a suitable environment for hardening treatment, so that the capsule shell is fully sealed, and the oligosaccharide selenium Ganoderma lucidum capsule is obtained. Step 5, Packaging: Pack the capsules containing powder to protect the quality of the capsules and facilitate storage and transportation.

[0020] The collagen extraction process is as follows: A1. Select fresh, uncontaminated cod skin, thaw it, wash it thoroughly to remove blood and impurities, and then mince it mechanically; Cod skin should be stored at low temperatures after passing further inspection to prevent premature protein degradation or spoilage.

[0021] A2, steam at 100℃ for 30 minutes, then add 1% pepsin, according to the ratio of raw materials to water. Adjust the pH to 2-3 at a ratio of 1:10, and react at around 37°C for 6 hours to fully hydrolyze the collagen into low molecular weight peptides. During the steaming or cooking stage, even heating is required to ensure that each piece of raw material is heated uniformly. A3. Adjust the pH of the solution in A2 to 6-7 to inactivate enzyme activity, and then add diatomaceous earth for decolorization and deodorization treatment; A4, after being filtered to remove suspended solids, is concentrated under reduced pressure at 0.08-0.1 MPa and 50-60℃. A5 involves instantaneously sterilizing the concentrated solution at 110-122℃ for 15 seconds, and finally producing powder by spray drying (inlet air temperature 140-180℃, outlet air temperature 70-85℃).

[0022] The concentration step must be closely integrated with the subsequent spray drying to avoid secondary contamination.

[0023] Collagen primarily provides structural support, but its hydrolysis products (low-molecular-weight peptides) can act as signaling molecules, promoting intercellular communication and thus regulating local immune responses to some extent. At the same time, collagen can improve the skin barrier, indirectly enhancing the body's defense against external pathogens.

[0024] The chitosan oligosaccharide is prepared by enzymatic hydrolysis of chitosan, and after enzymatic hydrolysis, it is prepared into a dry powder by filtration, concentration and spray drying (inlet air temperature set at 190-210℃, outlet air temperature at 75-85℃). During the enzymatic hydrolysis stage, a combination of enzymes (cellulase and papain) is required. The reaction is carried out at 50°C and pH 5.0-5.5 for 5 hours. Buffer solution can be used to maintain pH stability during this stage. Molecular weight distribution is monitored by sampling regularly to ensure that the target range of oligosaccharides is reached.

[0025] Chitosan oligosaccharide is produced by enzymatic degradation of chitosan. It has a lower molecular weight and better water solubility, and can be utilized by intestinal microorganisms. At the same time, chitosan oligosaccharide is an oligosaccharide that can promote the growth of probiotics, improve the balance of intestinal flora, and thus enhance the intestinal barrier function. Furthermore, chitosan oligosaccharide can indirectly stimulate the intestinal mucosal immune response and enhance the body's overall immunity.

[0026] The extraction process of the Ganoderma lucidum extract is as follows: B1. Ganoderma lucidum raw material is crushed and then mixed with eight times the amount of water. B2 was extracted twice at 100℃, with the first extraction lasting 3 hours and the second extraction lasting 2 hours, to ensure full dissolution of the active ingredients. During the extraction process, trace amounts of acid or alkali were added to adjust the pH and further optimize the dissolution rate of the active ingredients. B3, the extract was filtered to remove solid impurities, and then the solution was concentrated under reduced pressure; B4. Add 75% ethanol to the concentrate to allow polysaccharides and other impurities to settle, and let stand for 14 hours. B5 was separated and precipitated by vacuum filtration, then dried at 55℃ and -0.05MPa to obtain a stable powder. Finally, it was pulverized and sieved to ensure uniform particle size.

[0027] By extracting Ganoderma lucidum, the extract contains polysaccharides and triterpenoids. These active ingredients can significantly enhance the activity of immune cells in the body, improve the body's ability to recognize and eliminate pathogenic microorganisms and tumor cells, and the components of Ganoderma lucidum can have a certain inhibitory effect on tumor cells by regulating cell apoptosis pathways, inhibiting tumor angiogenesis, and regulating cell cycle.

[0028] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0029] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. An oligosaccharide selenium Ganoderma lucidum capsule that enhances immunity and inhibits tumors, characterized in that: The oligosaccharide selenium Ganoderma lucidum capsule consists of a capsule shell and capsule contents, wherein the capsule contents are made from the following raw materials in parts by weight: 150-160 parts collagen, 80-85 parts chitosan oligosaccharide, 110-130 parts Ganoderma lucidum extract, 45-55 parts selenium-enriched yeast, 50-80 parts maltodextrin, and 7-10 parts magnesium stearate.

2. The oligosaccharide selenium Ganoderma lucidum capsule for enhancing immunity and inhibiting tumors according to claim 1, characterized in that: The capsule shell is composed of the following raw materials in parts by weight: 80-100 parts gelatin, 50-65 parts glycerin, and 20-80 parts water.

3. A method for preparing oligosaccharide selenium Ganoderma lucidum capsules that enhance immunity and inhibit tumors, characterized in that: The specific steps of this method are as follows: Step 1, Raw material production and screening: Produce raw material powders of collagen, chitosan oligosaccharide and Ganoderma lucidum extract respectively, and screen each raw material powder to remove impurities and particles that do not meet the particle size requirements to ensure the quality of raw materials; Step 2, Uniform Mixing: According to the design formula of each capsule, accurately weigh the screened raw material powders and mix them thoroughly using a mixer to ensure that the components are evenly distributed. Step 3, Capsule filling: Prepare capsule shells and use a capsule filling machine to precisely fill the capsules with the uniformly mixed powder according to the predetermined dosage to complete the capsule filling process; Step 4, Packaging: Pack the capsules containing powder to protect their quality and facilitate storage and transportation.

4. The preparation method of the oligosaccharide selenium Ganoderma lucidum capsule for enhancing immunity and inhibiting tumors according to claim 3, characterized in that: The collagen extraction process is as follows: A1. Select fresh, uncontaminated cod skin, thaw it, wash it thoroughly to remove blood and impurities, and then mince it mechanically; A2, steam at 100℃ for 30 minutes, then add 1% pepsin, according to the ratio of raw materials to water. Adjust the pH to 2-3 at a ratio of 1:10, and react at around 37°C for 6 hours to fully hydrolyze the collagen into low molecular weight peptides. A3. Adjust the pH of the solution in A2 to 6-7 to inactivate enzyme activity, and then add diatomaceous earth for decolorization and deodorization treatment; A4, after being filtered to remove suspended solids, is concentrated under reduced pressure at 0.08-0.1 MPa and 50-60℃. A5 involves sterilizing the concentrated solution at 110-122℃ for 15 seconds, and then obtaining powder through a spray drying mechanism.

5. The preparation method of the oligosaccharide selenium Ganoderma lucidum capsule for enhancing immunity and inhibiting tumors according to claim 3, characterized in that: The chitosan oligosaccharide is prepared by enzymatic hydrolysis of chitosan, and after enzymatic hydrolysis, it is prepared into a dry powder by filtration, concentration and spray drying. In the enzymatic hydrolysis stage, a compound enzyme is required, and the reaction is carried out for 5 hours at a temperature of 50℃ and a pH of 5.0-5.

5.

6. The method for preparing an oligosaccharide selenium Ganoderma lucidum capsule for enhancing immunity and inhibiting tumors according to claim 3, characterized in that: The extraction process of the Ganoderma lucidum extract is as follows: B1. Ganoderma lucidum raw material is crushed and then mixed with eight times the amount of water. B2 was extracted twice at 100℃, with the first extraction lasting 3 hours and the second extraction lasting 2 hours, to ensure that the active ingredients were fully dissolved. B3, the extract was filtered to remove solid impurities, and then the solution was concentrated under reduced pressure; B4. Add 75% ethanol to the concentrate to allow polysaccharides and other impurities to settle, and let stand for 14 hours. B5 was separated and precipitated by vacuum filtration, then dried at 55℃ and -0.05MPa to obtain a stable powder. Finally, it was pulverized and sieved to ensure uniform particle size.

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