Oral liquid capable of promoting qi-blood dual-supplement easy absorption and preparation process thereof

Abstract

The application belongs to the field of traditional Chinese medicine preparations, and particularly relates to an oral liquid capable of promoting easy absorption of qi and blood tonification and a preparation process thereof. The preparation process comprises the following steps: preparation of an absorption promoting agent, preparation of a stabilizing and compatible agent, steaming and extraction of traditional Chinese medicinal materials, and preparation of the oral liquid. The absorption promoting agent is prepared by enzymolysis of mixed slurry of Adiantum capillus-veneris flowers and liquidambar resin using cellulose-pectin complex enzyme, and the absorption promoting agent is obtained by freeze-drying of the prepared liquidambar resin-Adiantum capillus-veneris enzymolysis liquid. The absorption promoting agent is added in the preparation process of the oral liquid, so that the absorption rate of effective components of Lycium chinense, Poria cocos, jujube and longan aril in the intestinal tract is improved, and the effect of the oral liquid capable of promoting easy absorption of qi and blood tonification on blood and qi tonification of human bodies is improved.

Description

TECHNICAL FIELD

[0001] The application belongs to the field of traditional Chinese medicine preparations, and particularly relates to an oral liquid capable of promoting easy absorption of qi and blood tonification and a preparation process thereof. BACKGROUND

[0002] Qi and blood deficiency as a common syndrome in traditional Chinese medicine shows a significant upward trend in modern fast-paced life. Long-term qi and blood deficiency can cause chain-like decline of body functions. Clinical observation shows that patients are often accompanied by persistent fatigue, immune cell activity inhibition and microcirculation disorder, and severe cases can even induce the risk of cardiovascular and cerebrovascular events. Therefore, there are many qi and blood tonifying health products on the current market.

[0003] Among the qi and blood tonifying health products, oral liquid health products are favored by people because of their convenience. In order to improve the effect of health products, many enterprises add a large amount of nutritional ingredients to the oral liquid. However, the hydrophobic components in the oral liquid are prone to phase separation and precipitation, which makes it difficult for some components to be effectively absorbed and utilized. In addition, some effective components are easily destroyed during high-temperature sterilization. At the same time, due to the low active transport efficiency of intestinal epithelial cells to some nutritional ingredients, even if a large amount of nutritional ingredients are added to the product, the effect of qi and blood tonification still cannot meet the expected problem. SUMMARY

[0004] In order to solve the above technical defects, the application researches a preparation process of an oral liquid capable of promoting easy absorption of qi and blood tonification. The prepared oral liquid capable of promoting easy absorption of qi and blood tonification has high storage stability and can improve the absorption and utilization effect of effective components by the human gastrointestinal tract, and has excellent qi and blood tonifying capacity.

[0005] A preparation process of an oral liquid capable of promoting easy absorption of qi and blood tonification, comprising the following steps:

[0006] S1: preparation of an absorption-promoting agent

[0007] After the tolu balsam is melted and impurities are removed, impurity-removed tolu balsam is obtained. Fresh Asparagus setaceus flowers are washed clean and then added to the impurity-removed tolu balsam and water to perform homogenization. Then, cellulose-pectin complex enzyme is used for constant-temperature vibration enzymolysis to obtain tolu balsam-Asparagus setaceus flower enzymolysis product. After cooling and centrifugation, the tolu balsam-Asparagus setaceus flower enzymolysis liquid is collected and freeze-dried to obtain the absorption-promoting agent.

[0008] S2: preparation of a stable compatibility agent

[0009] The defatted dried powder of the bee pupa of the golden ringed wasp is dissolved in deionized water, and a compound protease prepared by mixing alkaline protease and papain at a mass ratio of 1: (1-1.5) is used for enzymolysis. After enzyme inactivation and pH adjustment, centrifugation is performed, and the supernatant is collected, frozen and dried, dispersed in deionized water, adjusted to pH 8-9, heated to 75-80℃ for 20-25 min, then Tween 80 and polyamide-amine dendrimer are added and the pH is adjusted to neutral, and the mixture is cooled to room temperature to obtain a stable compatibilizer.

[0010] S3: Steaming extraction of medicinal materials and preparation of oral liquid

[0011] The wolfberry, tuckahoe, jujube and longan meat are crushed and steamed with white sugar, then extracted twice by alcohol extraction, and the extract is concentrated under reduced pressure. Chitosan flocculation is added to obtain a purified extract of medicinal materials. The stable compatibilizer is added to the purified extract of medicinal materials, diluted, and then the absorption promoter and potassium sorbate are added. After uniform stirring in a water bath, it is sterilized by filling to obtain an oral liquid that promotes the absorption of qi and blood.

[0012] Further, the preparation of the absorption promoter in step S1 specifically includes the following steps:

[0013] S1.1: The liquidambar resin is heated to melt in a heating pot at 80-85℃, then sieved through a 100-120 mesh sieve to obtain a deimpure liquidambar resin;

[0014] S1.2: Fresh and non-rotten Asparagus setaceus flowers are selected, washed with clean water, then placed in a high-speed blender with the deimpure liquidambar resin at a mass ratio of 1: (0.3-0.5). After adding clean water at a solid-liquid ratio of 1: (5-7) g / mL, the mixture is crushed and homogenized to obtain a liquidambar resin-Asparagus setaceus slurry. Citric acid is added to adjust the pH to 4-4.5, and cellulose-pectin complex enzyme is added at 0.05-0.1% of the mass of the liquidambar resin-Asparagus setaceus slurry. The temperature is adjusted to 55-60℃, and the mixture is enzymolyzed for 3-3.5 h under constant temperature oscillation to obtain a liquidambar resin-Asparagus setaceus enzymolyzate.

[0015] S1.3: The liquidambar resin-Asparagus setaceus enzymolyzate is heated in a boiling water bath for 8-10 min, cooled to room temperature, then transferred to a centrifuge and centrifuged at a speed of 4500-5000 rpm for 15-20 min. The supernatant is collected to obtain a liquidambar resin-Asparagus setaceus enzymolyzate, which is freeze-dried to obtain an absorption promoter.

[0016] Further, the preparation of the stable compatibilizer in step S2 specifically includes the following steps:

[0017] S2.1: The dried powder of Vespa ducalis pupae is extracted with ether by Soxhlet extraction for 1.5-2 h to remove fat, and then dried in a vacuum drying oven to constant weight to completely remove ether, to obtain defatted bee pupa protein. The defatted bee pupa protein is dissolved in deionized water at a solid-liquid ratio of 1:(25-30) g / mL, the pH is adjusted to 7-8, and then 0.4-0.6% of compound protease based on the mass of the defatted bee pupa protein is added, the temperature is adjusted to 45-50°C, and enzymolysis is performed for 4-4.5 h to obtain an enzymolysis liquid;

[0018] S2.2: The enzymolysis liquid is heated in a boiling water bath for 15-20 min to inactivate the enzyme, then cooled to room temperature and the pH is adjusted to neutral, and then centrifuged at a speed of 4000-4500 rpm for 15-20 min. The supernatant is collected and freeze-dried to obtain Vespa ducalis pupae peptide;

[0019] S2.3: The Vespa ducalis pupae peptide is dispersed in deionized water at a mass concentration of 2-3%, then placed at 4-5°C overnight, 1.5-2 mol / L sodium hydroxide solution is added to adjust the pH to 8-9, then heated to 75-80°C for 20-25 min, and after heating, a Vespa ducalis pupae dissociated peptide solution is obtained. The Vespa ducalis pupae dissociated peptide solution is mixed uniformly with Tween 80 and polyamide-amine dendrimer, then 1.5-2 mol / L hydrogen chloride solution is added to adjust the pH to neutral and stand to cool to room temperature to obtain a stable compatibilizer.

[0020] Further, the steaming extraction of medicinal materials and the preparation of oral liquid in step S3 specifically include the following steps:

[0021] S3.1: Mix the wolfberry fruit, Poria cocos, jujube and longan meat in a mass ratio of 1:(0.3-0.5):(1-1.5):(2-3) and crush them in a blender, then add 10-12 wt% of white granulated sugar and mix uniformly, then transfer to a bamboo steamer for steaming for 40-45 h to obtain steamed medicinal materials;

[0022] S3.2: Mix the steamed medicinal materials and 70-75% ethanol aqueous solution in a mass ratio of 1:(14-15) and place them in a reflux device for reflux extraction at 80-85°C for 2-2.5 h, then separate the extract and residue by filtration, mix the residue and ethanol aqueous solution under the same conditions and repeat the reflux extraction, filter and combine the extract from the two reflux extractions to obtain a medicinal material extract, then add chitosan and stir uniformly to obtain a chitosan mass concentration of 5-6%, and then perform flocculation at 32-35°C for 4-5 h to obtain a purified medicinal material extract;

[0023] S3.3: 10-12wt% of the stable compatibilizer is added into the purified extract of traditional Chinese medicinal materials, and stirred uniformly, then 5-8wt% of the absorption promoting agent and 0.1-0.2wt% of potassium sorbate are added, and the mixture is stirred uniformly under the condition of water bath at 90-92℃, and after standing and cooling, the filling is carried out, and the sterilization is carried out at 115℃ for 30min, so that the oral liquid capable of promoting easy absorption of qi and blood tonification is obtained.

[0024] Further, the cellulose-pectin complex enzyme in step S1.2 is mixed by pectinase and cellulase at a mass ratio of 1:(1-1.2).

[0025] Further, the complex protease in step S2.1 is mixed by alkaline protease and papain at a mass ratio of 1:(1-1.5).

[0026] Further, the polyamide-amine dendrimer in step S2.3 is PAMAM-G4-NH2.

[0027] Further, after the Tween 80 and the polyamide-amine dendrimer are added into the solution of the dissociated peptide of the bee pupa of Ampulex, the solution is stirred uniformly, the mass concentration of the Tween 80 in the solution of the dissociated peptide of the bee pupa of Ampulex is 0.4-0.6%, and the mass concentration of the polyamide-amine dendrimer is 12-15%.

[0028] Further, the condition of the reduced pressure concentration in step S3.2 is 60-65℃, and the ethanol is recovered until no odor.

[0029] An oral liquid capable of promoting easy absorption of qi and blood tonification is prepared by the preparation process of the oral liquid capable of promoting easy absorption of qi and blood tonification.

[0030] Beneficial effects are: 1, the mixed slurry prepared from the lycopene and the toona sinensis by using the cellulose-pectin complex enzyme is enzymolyzed, the absorption promoting agent is obtained after the toona sinensis-lycopene enzymolysis solution is freeze-dried, and the absorption promoting agent is added in the preparation process of the oral liquid, so that the absorption rate of the effective components of the wolfberry fruit, the poria cocos, the jujube and the longan aril in the intestinal tract is improved, and the effect of the oral liquid capable of promoting easy absorption of qi and blood tonification on blood and qi tonification is improved.

[0031] 2. This invention utilizes a complex protease, composed of alkaline protease and papain mixed in a mass ratio of 1:(1-1.5), to enzymatically hydrolyze defatted bee pupa protein, thereby obtaining amphiphilic and self-assembling vesicle pupa peptides. The vesicle pupa peptides are then placed in a high-temperature and alkaline environment to dissociate and expand the aggregated peptides, yielding a vesicle pupa dissociated peptide solution. Tween 80 and a polyamide-amine dendritic polymer are added and mixed thoroughly. The solution is then pH neutralized and allowed to cool. During this process, the fully dissociated vesicle pupa dissociated peptides self-assemble and attach to the polyamide-amine dendritic polymer, forming a regularly arranged sheet structure. This results in numerous amphiphilic sheet-like stacked cavities on the polyamide-amine dendritic polymer, significantly improving the compatibility between water-insoluble and easily stratified components in the oral liquid and the solution, thus giving the easily absorbed oral liquid that promotes blood and qi replenishment high storage stability.

[0032] 3. This invention involves crushing and mixing wolfberry, poria cocos, jujube, and longan pulp, then steaming them together, and then simultaneously extracting the steamed medicinal materials using an alcohol extraction method. By mixing and steaming the four ingredients together to carry out the Maillard reaction, the content of effective components in wolfberry, poria cocos, jujube, and longan pulp can be effectively increased, thereby improving their effects of replenishing qi and blood. Detailed Implementation

[0033] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0034] Example 1

[0035] A preparation process for an oral liquid that promotes the replenishment of both qi and blood and is easily absorbed includes the following steps:

[0036] S1: Preparation of absorption enhancer

[0037] S1.1: Heat the styrax resin in a pot at 80°C until it melts, then pass it through a 100-mesh sieve to obtain impurity-free styrax resin;

[0038] S1.2: Select fresh asparagus flowers without rot, wash them clean with water, and then place them in a high-speed mixer with impurity-removed liquid resin at a mass ratio of 1:0.3. Add water at a material-liquid ratio of 1:5 g / mL and then crush and homogenize to obtain liquid resin-asparagus flower slurry. Add citric acid to adjust the pH to 4, and then add 0.05% of cellulose-pectin complex enzyme (composed of pectinase and cellulase mixed at a mass ratio of 1:1) to the liquid resin-asparagus flower slurry. Adjust the temperature to 55℃ and perform constant temperature shaking enzymatic hydrolysis for 3 hours to obtain liquid resin-asparagus flower enzymatic hydrolysate.

[0039] S1.3: The liquid resin-asparagus flower enzymatic hydrolysate was heated in a boiling water bath for 8 minutes, cooled to room temperature, transferred to a centrifuge, and centrifuged at 4500 rpm for 15 minutes. The supernatant was collected to obtain the liquid resin-asparagus flower enzymatic hydrolysate, which was then freeze-dried to obtain the absorption promoter.

[0040] S2: Preparation of Stabilizing Compatibilizer

[0041] S2.1: Take freeze-dried powder of golden ringed wasp pupae, extract it with ether as solvent using Soxhlet extraction for 1.5 h to remove fat, then place it in a vacuum drying oven to dry to constant weight, completely remove ether, and obtain defatted wasp pupae protein. Dissolve the defatted wasp pupae protein in deionized water at a material-to-liquid ratio of 1:25 g / mL, adjust the pH to 7, and then add a complex protease accounting for 0.4% of the defatted wasp pupae protein. The complex protease is a mixture of alkaline protease and papain in a mass ratio of 1:1. Adjust the temperature to 45℃ and enzymatically hydrolyze for 4 h to obtain the enzymatic hydrolysate.

[0042] S2.2: The enzyme hydrolysate was heated in a boiling water bath for 15 min to inactivate the enzyme, then cooled to room temperature and the pH was adjusted to neutral. It was then centrifuged at 4000 rpm for 15 min. The supernatant was collected and freeze-dried to obtain the golden ring wasp pupa peptide.

[0043] S2.3: The *Vespa mandshurica* pupal peptide was dispersed in deionized water at a mass concentration of 2%, and then incubated overnight at 4°C. The pH was adjusted to 8 by adding 1.5 mol / L sodium hydroxide solution, and then heated to 75°C for 20 min. After heating, a *Vespa mandshurica* pupal dissociation peptide solution was obtained. Tween 80 and PAMAM-G4-NH2 were added to the *Vespa mandshurica* pupal dissociation peptide solution and mixed thoroughly. The mass concentration of Tween 80 in the *Vespa mandshurica* pupal dissociation peptide solution was 0.4%, and the mass concentration of PAMAM-G4-NH2 was 12%. Then, 1.5 mol / L hydrogen chloride solution was added to adjust the pH to neutral, and the solution was allowed to stand and cool to room temperature to obtain a stable compatibilizer.

[0044] S3: Steaming and extraction of Chinese medicinal herbs and preparation of oral liquids

[0045] S3.1: Mix wolfberry, poria cocos, jujube and longan pulp in a mass ratio of 1:0.3:1:2 and crush them in a mixer. Then add 10wt% white sugar and mix well. Then transfer to a bamboo steamer and steam for 40 hours to obtain steamed Chinese medicinal materials.

[0046] S3.2: The steamed Chinese medicinal materials and a 70% ethanol aqueous solution were mixed at a mass ratio of 1:14 and placed in a reflux device for reflux extraction at 80°C for 2 hours. The extract and residue were then separated by filtration. The residue and ethanol aqueous solution were mixed under the same conditions and the reflux extraction was repeated once. The extracts from the two reflux extractions were combined by filtration to obtain the Chinese medicinal material extract. The ethanol was concentrated under reduced pressure at 60°C until it was tasteless. Chitosan was then added and stirred evenly to make the mass concentration of chitosan 5%. The mixture was flocculated at 32°C for 4 hours to obtain the purified Chinese medicinal material extract.

[0047] S3.3: Add 10wt% of a stable compatibilizer to the purified extract of Chinese medicinal materials and stir evenly. Then add 5wt% of an absorption promoter and 0.1wt% of potassium sorbate. Stir evenly in a water bath at 90℃, let stand and cool, then fill into containers and sterilize at 115℃ for 30 minutes to obtain an oral liquid that promotes the replenishment of both qi and blood and is easily absorbed.

[0048] Example 2

[0049] A preparation process for an oral liquid that promotes the replenishment of both qi and blood and is easily absorbed includes the following steps:

[0050] S1: Preparation of absorption enhancer

[0051] S1.1: Heat the styrax resin in a heating pot at 83°C until it melts, and then pass it through a 110-mesh sieve to obtain impurity-free styrax resin;

[0052] S1.2: Select fresh asparagus flowers without rot, wash them clean with water, and then place them in a high-speed mixer with purified liquid resin at a mass ratio of 1:0.4. Add water at a material-to-liquid ratio of 1:6 g / mL and then crush and homogenize to obtain liquid resin-asparagus flower slurry. Add citric acid to adjust the pH to 4.5, and then add 0.08% of cellulose-pectin complex enzyme (composed of pectinase and cellulase mixed at a mass ratio of 1:1.1) to the liquid resin-asparagus flower slurry. Adjust the temperature to 57℃ and hydrolyze at a constant temperature with shaking for 3.3 h to obtain liquid resin-asparagus flower hydrolysate.

[0053] S1.3: The liquid resin-asparagus flower enzymatic hydrolysate was heated in a boiling water bath for 9 minutes, cooled to room temperature, transferred to a centrifuge, and centrifuged at 4700 rpm for 17 minutes. The supernatant was collected to obtain the liquid resin-asparagus flower enzymatic hydrolysate, which was then freeze-dried to obtain the absorption promoter.

[0054] S2: Preparation of Stabilizing Compatibilizer

[0055] S2.1: Take freeze-dried powder of golden ringed wasp pupae, extract it with ether as solvent using Soxhlet extraction for 1.8 h to remove fat, then place it in a vacuum drying oven to constant weight, completely remove ether, and obtain defatted wasp pupa protein. Dissolve the defatted wasp pupa protein in deionized water at a material-to-liquid ratio of 1:27 g / mL, adjust the pH to 7.5, and then add a complex protease accounting for 0.5% of the defatted wasp pupa protein. The complex protease is a mixture of alkaline protease and papain at a mass ratio of 1:1.3. Adjust the temperature to 48℃ and enzymatically hydrolyze for 4.2 h to obtain the enzymatic hydrolysate.

[0056] S2.2: The enzyme hydrolysate was heated in a boiling water bath for 18 min to inactivate the enzyme, then cooled to room temperature and the pH was adjusted to neutral. It was then centrifuged at 4250 rpm for 17 min. The supernatant was collected and freeze-dried to obtain the golden ring wasp pupa peptide.

[0057] S2.3: The *Vespa mandshurica* pupal peptide was dispersed in deionized water at a mass concentration of 2.5%, and then incubated overnight at 5°C. The pH was adjusted to 8.5 by adding 1.8 mol / L sodium hydroxide solution, and then heated to 78°C for 22 min. After heating, a *Vespa mandshurica* pupal dissociation peptide solution was obtained. Tween 80 and PAMAM-G4-NH2 were added to the *Vespa mandshurica* pupal dissociation peptide solution and mixed thoroughly. The mass concentration of Tween 80 in the *Vespa mandshurica* pupal dissociation peptide solution was 0.5%, and the mass concentration of PAMAM-G4-NH2 was 13%. Then, 1.8 mol / L hydrogen chloride solution was added to adjust the pH to neutral, and the solution was allowed to stand and cool to room temperature to obtain a stable compatibilizer.

[0058] S3: Steaming and extraction of Chinese medicinal herbs and preparation of oral liquids

[0059] S3.1: Mix wolfberry, poria cocos, jujube and longan pulp in a mass ratio of 1:0.4:1.3:2.5 and crush them in a mixer. Then add 11wt% white sugar and mix well. Then transfer to a bamboo steamer for steaming for 43 hours to obtain steamed Chinese medicinal materials.

[0060] S3.2: The steamed Chinese medicinal materials and a 73% ethanol aqueous solution were mixed at a mass ratio of 1:14.5 and placed in a reflux apparatus. The mixture was refluxed at 82°C for 2 hours. The extract and residue were then separated by filtration. The residue and ethanol aqueous solution were mixed under the same conditions and the reflux extraction was repeated once. The extracts from the two reflux extractions were combined by filtration to obtain the Chinese medicinal material extract. The ethanol was concentrated under reduced pressure at 62.5°C until it was tasteless. Chitosan was then added and stirred until the mass concentration of chitosan was 5.5%. The mixture was flocculated at 34°C for 4.5 hours to obtain the purified Chinese medicinal material extract.

[0061] S3.3: Add 11wt% of a stable compatibilizer to the purified extract of Chinese medicinal materials and stir evenly. Then add 7wt% of an absorption promoter and 0.15wt% of potassium sorbate. Stir evenly in a water bath at 91℃, let stand and cool, then fill into containers and sterilize at 115℃ for 30 minutes to obtain an oral liquid that promotes the replenishment of qi and blood and is easily absorbed.

[0062] Example 3

[0063] A preparation process for an oral liquid that promotes the replenishment of both qi and blood and is easily absorbed includes the following steps:

[0064] S1: Preparation of absorption enhancer

[0065] S1.1: Heat the styrax resin in a pot at 85°C until it melts, then pass it through a 120-mesh sieve to obtain impurity-free styrax resin;

[0066] S1.2: Select fresh asparagus flowers without rot, wash them clean with water, and then place them in a high-speed mixer with impurity-removed liquid resin at a mass ratio of 1:0.5. Add water at a material-liquid ratio of 1:7 g / mL and then crush and homogenize to obtain liquid resin-asparagus flower slurry. Add citric acid to adjust the pH to 4.5, and then add 0.1% of the mass of liquid resin-asparagus flower slurry containing cellulose-pectin complex enzyme. The cellulose-pectin complex enzyme is composed of pectinase and cellulase mixed at a mass ratio of 1:1.2. Adjust the temperature to 60℃ and perform constant temperature shaking enzymatic hydrolysis for 3.5 h to obtain liquid resin-asparagus flower enzymatic hydrolysate.

[0067] S1.3: The liquid resin-asparagus flower enzymatic hydrolysate was heated in a boiling water bath for 10 minutes, cooled to room temperature, transferred to a centrifuge, and centrifuged at 5000 rpm for 20 minutes. The supernatant was collected to obtain the liquid resin-asparagus flower enzymatic hydrolysate, which was then freeze-dried to obtain the absorption promoter.

[0068] S2: Preparation of Stabilizing Compatibilizer

[0069] S2.1: Take freeze-dried powder of golden ringed wasp pupae, extract it with ether as solvent using Soxhlet extraction for 2 hours to remove fat, then place it in a vacuum drying oven to constant weight, completely remove ether, and obtain defatted wasp pupa protein. Dissolve the defatted wasp pupa protein in deionized water at a material-to-liquid ratio of 1:30 g / mL, adjust the pH to 8, and then add a complex protease accounting for 0.6% of the defatted wasp pupa protein. The complex protease is a mixture of alkaline protease and papain at a mass ratio of 1:1.5. Adjust the temperature to 50℃ and enzymatically hydrolyze for 4.5 hours to obtain the enzymatic hydrolysate.

[0070] S2.2: The enzyme hydrolysate was heated in a boiling water bath for 20 min to inactivate the enzyme, then cooled to room temperature and the pH was adjusted to neutral. It was then centrifuged at 4500 rpm for 20 min. The supernatant was collected and freeze-dried to obtain the golden ring wasp pupa peptide.

[0071] S2.3: The *Vespa mandshurica* pupal peptide was dispersed in deionized water at a mass concentration of 3%, and then incubated overnight at 5°C. A 2 mol / L sodium hydroxide solution was added to adjust the pH to 9, and the solution was heated to 80°C for 25 min. After heating, a *Vespa mandshurica* pupal dissociation peptide solution was obtained. Tween 80 and PAMAM-G4-NH2 were added to the *Vespa mandshurica* pupal dissociation peptide solution and mixed thoroughly. The mass concentration of Tween 80 in the *Vespa mandshurica* pupal dissociation peptide solution was 0.6%, and the mass concentration of PAMAM-G4-NH2 was 15%. A 2 mol / L hydrogen chloride solution was then added to adjust the pH to neutral, and the solution was allowed to cool to room temperature to obtain a stable compatibilizer.

[0072] S3: Steaming and extraction of Chinese medicinal herbs and preparation of oral liquids

[0073] S3.1: Mix wolfberry, poria cocos, jujube and longan pulp in a mass ratio of 1:0.5:1.5:3 and crush them in a mixer. Then add 12wt% white sugar and mix well. Then transfer to a bamboo steamer for steaming for 45 hours to obtain steamed Chinese medicinal materials.

[0074] S3.2: The steamed Chinese medicinal materials and a 75% ethanol aqueous solution were mixed at a mass ratio of 1:15 and placed in a reflux device for reflux extraction at 85°C for 2.5 hours. The extract and residue were then separated by filtration. The residue and ethanol aqueous solution were mixed under the same conditions and the reflux extraction was repeated once. The extracts from the two reflux extractions were combined by filtration to obtain the Chinese medicinal material extract. The ethanol was concentrated under reduced pressure at 65°C until it was tasteless. Chitosan was then added and stirred evenly to make the mass concentration of chitosan 6%. The mixture was flocculated at 35°C for 5 hours to obtain the purified Chinese medicinal material extract.

[0075] S3.3: Add 12wt% of a stable compatibilizer to the purified extract of Chinese medicinal materials and stir evenly. Then add 8wt% of an absorption promoter and 0.2wt% of potassium sorbate. Stir evenly in a water bath at 92℃, let stand and cool, then fill into containers and sterilize at 115℃ for 30 minutes to obtain an oral liquid that promotes the replenishment of both qi and blood and is easily absorbed.

[0076] Comparative Example 1

[0077] The difference from Example 1 is that step S1 was removed in this comparative example, and no absorption promoter was added in step S3.3. All other specific implementation methods are the same as in Example 1.

[0078] Comparative Example 2

[0079] The difference from Example 1 is that step S2 is removed in this comparative example, and the stabilizing compatibilizer in step S3.3 is replaced with Tween 80. The amount of Tween 80 added accounts for 1.3 wt% of the purified extract of Chinese medicinal materials. All other specific implementation methods are the same as in Example 1.

[0080] Comparative Example 3

[0081] The difference from Example 1 is that the golden ring wasp pupa peptide in step S2.3 is replaced with an equal mass of PAMAM-G4-NH2, while the rest of the specific implementation is the same as in Example 1.

[0082] Comparative Example 4

[0083] The difference from Example 1 is that the wolfberry, poria, jujube and longan pulp were not steamed in this comparative example. Instead, the wolfberry, poria, jujube and longan pulp were crushed in a mass ratio of 1:0.3:1:2, and 10wt% white sugar was added and mixed evenly to obtain the mixed Chinese medicinal materials. The steamed Chinese medicinal materials in step S3.2 were replaced with the mixed Chinese medicinal materials. All other specific implementation methods are the same as in Example 1.

[0084] Comparative Example 5

[0085] The difference from Example 1 is that step S3.1 of this comparative example is as follows: Weigh wolfberry, poria cocos, jujube and longan pulp in a mass ratio of 1:0.3:1:2, crush them separately, add 2.3wt% white sugar to wolfberry, 0.6wt% white sugar to poria cocos, 2.3wt% white sugar to jujube and 4.6wt% white sugar to longan pulp, and then transfer them to bamboo steamers for steaming for 45 hours. After steaming, mix them together to obtain steamed Chinese medicinal materials. The rest of the specific implementation methods are the same as those in Example 1.

[0086] Experiment 1: After anesthetizing the rats, a 3cm incision was made in the midline of the abdomen, and the rat's small intestine was quickly removed and rinsed clean in KR nutrient solution. A 10cm segment of the jejunum and ileum was cut off, and the intestine was turned inside out with a glass rod. The mucus was rinsed clean, one end was tied tightly with silk thread, and the other end was inserted into a plastic tube (4mm outer diameter) and tied with silk thread. 1mL of K-R nutrient solution was injected into the intestinal sac, and the everted intestinal sac was suspended in 45mL of KR nutrient solution at 37℃. 95% O2 and 5% CO2 were introduced to obtain experimental samples of everted intestinal sacs. A total of 12 samples were prepared and divided into 4 groups, with 3 samples in each group for use.

[0087] The oral solutions prepared in Examples 1-3 and Comparative Example 1 were used as oral solution samples. 1 mL of the oral solution sample was added to the KR nutrient solution of the everted intestinal sac experimental samples of different groups and mixed evenly. 0.2 mL of K-R nutrient solution was taken, and the glucose and amino acid contents were detected by high performance liquid chromatography and recorded as K1 and Q1, respectively. After 60 min, 0.2 mL of sample was taken from the plastic tube end of each everted intestinal sac experimental sample of each group, and the glucose content was detected by high performance liquid chromatography and recorded as K2 and Q2, respectively. The absorption rate of glucose by the intestinal sac was calculated as (K1-K2) / (T·A), and the absorption rate of amino acids by the intestinal sac was calculated as (Q1-Q2) / (T·A), where T is time and A is the effective area of ​​the intestinal sac for drug transport. The average value of the results for each group was taken, as shown in Table 1.

[0088] Table 1: Absorption rates of glucose and amino acids by everted intestinal sac

[0089]

[0090] As can be seen from the data in Table 1 of Examples 1-3 and Comparative Example 1, the absorption rate of glucose and amino acids in Examples 1-3 by the everted intestinal sac is much higher than that in Comparative Example 1. This proves that the absorption promoter prepared by this application using styrax resin and asparagus flower hydrolysate can improve the absorption rate of the effective components of wolfberry, poria cocos, jujube and longan pulp in the intestine.

[0091] The oral liquids prepared in Examples 1-3 and Comparative Examples 2-3 were used as oral liquid samples. Three portions of each were taken and placed at 40°C and 75% relative humidity for 6 months. The precipitation and stratification of each oral liquid were observed in the 3rd, 5th and 6th months. The results are shown in Table 2.

[0092] Table 2: Sedimentation and stratification phenomena of oral liquid after 6 months of storage

[0093]

[0094] As can be seen from the data in Examples 1-3 in Table 2, the oral liquid prepared in this application that promotes the absorption of both qi and blood has excellent storage stability. As can be seen from Comparative Example 2, the stabilizing compatibilizer prepared in this application has a much higher stabilizing effect than Tween 80. As can be seen from Comparative Example 3, by preparing wasp pupa peptides, this application allows them to attach to the surface of polyamide-amine dendritic polymers during dissociation and self-assembly. The resulting stabilizing compatibilizer can significantly improve the compatibility between the water-insoluble and easily separated components in the oral liquid and the solution, thus giving the oral liquid that promotes the absorption of both qi and blood high storage stability.

[0095] The oral liquids prepared in Examples 1-3 and Comparative Examples 4-5, which promote the replenishment of qi and blood and are easily absorbed, were tested for polysaccharide and flavonoid content using high performance liquid chromatography. Three parallel tests were performed for each test, and the average value of the results was taken. The results are shown in Table 3.

[0096] Table 3: Polysaccharide and flavonoid content in oral liquid

[0097]

[0098] As can be seen from the data in Table 3 of Examples 1-3 and Comparative Examples 4-5, when Comparative Example 4 did not steam wolfberry, poria cocos, jujube, and longan pulp, the content of polysaccharides and flavonoids decreased significantly, indicating that steaming can increase the content of effective components in wolfberry, poria cocos, jujube, and longan pulp. In Comparative Example 5, when wolfberry, poria cocos, jujube, and longan pulp were not steamed together, the content of polysaccharides and flavonoids also decreased, proving that by steaming wolfberry, poria cocos, jujube, and longan pulp together to carry out Maillard reaction, the content of effective components can be further increased.

[0099] HepG2 cells and Caco-2 cells were seeded into 96-well plates, respectively, and the cell concentration was adjusted to 1×10⁻⁶. 4 / mL, and then incubated in a 37℃ incubator with 5% CO2 for 12h. Then, 2μL of culture medium containing 30% of the oral liquid prepared in Examples 1-3 that promotes the absorption of both qi and blood was added to each well, and negative control wells and blank zeroing wells were set up (the final concentration of DMSO in each well was 0.1%), and cultured for another 48h. The culture medium of each well was aspirated, 20μL of 5mg / mL LMTT solution was added, and cultured for another 4h. After discarding the supernatant, the crystals were dissolved by shaking with 150μL LMTSO for 10min. The optical density (OD) value was measured at 490nm using an ELISA reader. The effect of the oral liquid that promotes the absorption of both qi and blood on the proliferation of HepG2 cells and Caco-2 cells was determined by the MTT assay. Cell viability (%) = (OD value of drug well / OD value of negative control well) × 100%. The results are shown in Table 4.

[0100] Table 4: Cell viability after oral liquid treatment

[0101]

[0102] As can be seen from the data in Examples 1-3 in Table 4, the survival rate of HepG2 cells and Caco-2 cells after culturing with the oral liquid of this application is higher than 95%, proving that the oral liquid prepared in this application is safe and reliable and has no obvious cytotoxicity.

[0103] The above embodiments are merely illustrative of the principles and effects of the present invention and are not intended to limit the invention. Any person skilled in the art can modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by those skilled in the art without departing from the spirit and technical concept disclosed in the present invention should still be covered by the claims of the present invention.

Claims

1. A preparation process for an oral liquid that promotes the simultaneous replenishment of qi and blood and is easily absorbed, characterized in that, Includes the following steps: S1: Preparation of Absorption Enhancer After melting and removing impurities from the styrax resin, impurity-free styrax resin is obtained. Fresh asparagus flowers are washed clean and then added to water with the impurity-free styrax resin for homogenization. Citric acid is added to adjust the pH, and then cellulose-pectin complex enzyme is used for isothermal shaking enzymatic hydrolysis to obtain styrax resin-asparagus flower hydrolysate. The styrax resin-asparagus flower hydrolysate is placed in a boiling water bath and heated for 8-10 minutes. After cooling and centrifugation, the supernatant is collected to obtain styrax resin-asparagus flower hydrolysate. Then, it is freeze-dried to obtain an absorption promoter. S2: Preparation of Stabilizing Compatibilizer S2.1: Take freeze-dried powder of golden ring wasp pupae, extract it with ether as solvent using Soxhlet extraction for 1.5-2 hours to remove fat, then place it in a vacuum drying oven to dry to constant weight, completely remove ether, and obtain defatted wasp pupa protein. Dissolve the defatted wasp pupa protein in deionized water at a material-to-liquid ratio of 1:(25-30) g / mL, adjust the pH to 7-8, and then add a complex protease consisting of alkaline protease and papain, accounting for 0.4-0.6% of the defatted wasp pupa protein. Adjust the temperature to 45-50℃ and enzymatically hydrolyze for 4-4.5 hours to obtain the enzymatic hydrolysate. S2.2: Heat the enzymatic hydrolysate in a boiling water bath for 15-20 min to inactivate the enzyme, then cool it to room temperature and adjust the pH to neutral. Then place it in a centrifuge and centrifuge at 4000-4500 rpm for 15-20 min. Collect the supernatant and freeze-dry it to obtain the golden ring wasp pupa peptide. S2.3: Disperse the 2-3% (w / w) concentration of the vesicle pupal peptide in deionized water, then incubate overnight at 4-5°C. Adjust the pH to 8-9 by adding 1.5-2 mol / L sodium hydroxide solution, then heat to 75-80°C for 20-25 min. After heating, a vesicle pupal dissociation peptide solution is obtained. Add Tween 80 and polyamide-amine dendritic polymer PAMAM-G4-NH2 to the vesicle pupal dissociation peptide solution and mix thoroughly. Then add 1.5-2 mol / L hydrogen chloride solution to adjust the pH to neutral and allow to stand and cool to room temperature to obtain a stable compatibilizer. S3: Steaming and extraction of Chinese medicinal herbs and preparation of oral liquids After crushing wolfberry, poria cocos, jujube, and longan pulp, they are steamed with white sugar. Then, they are extracted twice by reflux using the alcohol extraction method. The extracts are combined and concentrated under reduced pressure until the ethanol is recovered and tasteless. Chitosan is then added for flocculation to obtain a purified extract of Chinese medicinal materials. A stabilizing compatibilizer is added to the purified extract of Chinese medicinal materials, and after dilution, an absorption promoter and potassium sorbate are added. After stirring evenly in a water bath, the mixture is filled and sterilized to obtain an oral liquid that promotes the replenishment of qi and blood and is easily absorbed.

2. The preparation process of an oral liquid that promotes the replenishment of both qi and blood and is easily absorbed, as described in claim 1, is characterized in that... Step S1, the preparation of the absorption enhancer, specifically includes the following steps: S1.1: Heat the styrax resin in a pot at 80-85℃ until it melts, then pass it through a 100-120 mesh sieve to obtain impurity-free styrax resin; S1.2: Select fresh asparagus flowers without rot, wash them clean with water, and then place them in a high-speed mixer with impurity-removed liquid resin at a mass ratio of 1:(0.3-0.5). Add water at a material-liquid ratio of 1:(5-7) g / mL and then crush and homogenize to obtain liquid resin-asparagus flower slurry. Add citric acid to adjust the pH to 4-4.5, and then add 0.05-0.1% of cellulose-pectin complex enzyme according to the mass of liquid resin-asparagus flower slurry. Adjust the temperature to 55-60℃ and perform constant temperature shaking enzymatic hydrolysis for 3-3.5h to obtain liquid resin-asparagus flower enzymatic hydrolysate. S1.3: Heat the liquid resin-asparagus flower enzymatic hydrolysate in a boiling water bath for 8-10 minutes, cool it to room temperature, transfer it to a centrifuge, centrifuge at 4500-5000 rpm for 15-20 minutes, collect the supernatant to obtain the liquid resin-asparagus flower enzymatic hydrolysate, and freeze-dry it to obtain the absorption promoter.

3. The preparation process of an oral liquid that promotes the replenishment of both qi and blood and is easily absorbed, as described in claim 1, is characterized in that... Step S3, the steaming and extraction of medicinal materials and the preparation of the oral liquid, specifically includes the following steps: S3.1: Mix wolfberry, poria cocos, jujube and longan pulp in a mass ratio of 1:(0.3-0.5):(1-1.5):(2-3) and crush them in a mixer. Then add 10-12wt% white sugar and mix evenly. Then transfer to a bamboo steamer for steaming for 40-45 hours to obtain steamed Chinese medicinal materials. S3.2: Mix the steamed Chinese medicinal materials and a 70-75% ethanol aqueous solution at a mass ratio of 1:(14-15) in a reflux reflux device and perform reflux extraction at 80-85℃ for 2-2.5 hours. Then filter to separate the extract and the residue. Under the same conditions, mix the residue and the ethanol aqueous solution and repeat the reflux extraction once. Filter and combine the extracts from the two reflux extractions to obtain the Chinese medicinal material extract. Add chitosan and stir evenly to make the chitosan mass concentration 5-6%. Flocculate at 32-35℃ for 4-5 hours to obtain the purified Chinese medicinal material extract. S3.3: Add 10-12wt% of a stable compatibilizer to the purified extract of Chinese medicinal materials and stir evenly. Then add 5-8wt% of an absorption promoter and 0.1-0.2wt% of potassium sorbate. Stir evenly in a water bath at 90-92℃, let stand and cool, then fill into containers and sterilize at 115℃ for 30 minutes to obtain an oral liquid that promotes the replenishment of both qi and blood and is easily absorbed.

4. The preparation process of an oral liquid that promotes the replenishment of both qi and blood and is easily absorbed, as described in claim 2, is characterized in that... The cellulose-pectin complex enzyme in step S1.2 is composed of pectinase and cellulase mixed in a mass ratio of 1:(1-1.2).

5. The preparation process of an oral liquid that promotes the replenishment of both qi and blood and is easily absorbed, as described in claim 1, is characterized in that... The complex protease in step S2.1 is a mixture of alkaline protease and papain in a mass ratio of 1:(1-1.5).

6. The preparation process of an oral liquid that promotes the replenishment of both qi and blood and is easily absorbed, as described in claim 1, is characterized in that... In step S2.3, Tween 80 and polyamide-amine dendritic polymer PAMAM-G4-NH2 are added to the hygroscopic peptide solution of wasp pupa and mixed evenly. The mass concentration of Tween 80 in the hygroscopic peptide solution of wasp pupa is 0.4-0.6%, and the mass concentration of polyamide-amine dendritic polymer PAMAM-G4-NH2 is 12-15%.

7. The preparation process of an oral liquid that promotes the simultaneous replenishment of qi and blood and is easily absorbed, as described in claim 3, is characterized in that... In step S3.2, the concentration under reduced pressure is carried out at 60-65°C until the ethanol is recovered and there is no odor.

8. An oral liquid that promotes the simultaneous replenishment of qi and blood and is easily absorbed, characterized in that, It is prepared by the preparation process of an oral liquid that promotes the replenishment of both qi and blood and is easily absorbed, as described in any one of claims 1-7.

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