Multi-effect skin care mask containing loropetalum chinense extract and preparation method of multi-effect skin care mask

Abstract

The invention belongs to the technical field of skin care products, and particularly relates to a multi-effect skin care mask containing loropetalum chinense extract and a preparation method of the multi-effect skin care mask. The mask liquid is prepared from, by mass, 2%-5% of loropetalum chinense extract, 5% of glycerinum, 0.1%-1% of sodium hyaluronate, 0.1%-1% of phenoxyethanol, 0.01%-0.05% of EDTA disodium, 0.05%-0.5% of citric acid / sodium citrate and the balance deionized water, and the multiple-effect skin care mask is excellent in ABTS free radical scavenging rate and tyrosinase inhibition rate, has the multiple effects of moisturizing, resisting oxidation and whitening and can be used for skin care. And a new thought is provided for development and utilization of loropetalum chinense.

Description

Technical Field

[0001] This invention relates to the field of skincare technology, and in particular to a multi-effect skincare mask containing Loropetalum chinense extract and its preparation method. Background Technology

[0002] In the modern beauty and skincare market, face masks are widely favored by consumers due to their convenience and significant skincare effects. With the fast pace of life and increasing environmental pressure, skin oxidation and pigmentation problems are becoming increasingly prominent. Consumers' functional demands for skincare products have expanded from basic moisturizing to multiple benefits such as anti-oxidation, whitening, and repair. Factors such as ultraviolet radiation, air pollution, and irregular sleep patterns can lead to excessive production of free radicals in the skin, accelerating the degradation of collagen and elastin fibers, thereby causing skin sagging, wrinkles, and excessive melanin deposition. Therefore, developing skincare products with both anti-oxidation and whitening effects has significant market importance and application value.

[0003] Currently, many facial mask products utilize plant extracts as functional ingredients to cater to consumers' pursuit of natural and green skincare. Among them, Loropetalum chinense var. rubrum, a traditional medicinal plant, has extracts rich in various bioactive components, primarily including flavonoids (such as quercetin, kaempferol, and their glycoside derivatives) and phenolic acids (such as gallic acid and ellagic acid). Studies show that these components possess significant antioxidant and whitening potential: flavonoids effectively scavenge free radicals, inhibit lipid peroxidation, and alleviate oxidative stress damage to skin cells; phenolic acids can inhibit tyrosinase activity, blocking melanin synthesis and accumulation, thereby improving uneven skin tone and pigmentation. Furthermore, Loropetalum chinense extract also has certain anti-inflammatory and soothing effects, helping to repair damaged skin barriers.

[0004] Although some progress has been made in the component research and in vitro activity evaluation of Loropetalum chinense extract, its development and application in facial mask products are still in their early stages. Most existing products focus only on adding a single type of active ingredient, failing to systematically integrate its potential for synergistic effects across multiple components. Furthermore, the extraction process suffers from low efficiency and easy loss of active ingredients. In addition, systematic and in-depth research is still lacking on the stability, skin compatibility, and scientific compatibility with other active ingredients (such as moisturizers and soothing agents) of this extract in actual formulations, preventing its comprehensive skincare value from being fully realized. Summary of the Invention

[0005] To address the aforementioned problems, and especially to address the shortcomings of existing technologies, this invention provides a multi-effect skincare mask containing Loropetalum chinense extract and its preparation method.

[0006] To achieve the above objectives, the present invention employs the following technical means: On the one hand, this application provides a multi-effect skin care mask containing Loropetalum chinense extract, comprising a mask liquid and a mask sheet, wherein the mass percentage content of each component in the mask liquid is as follows: Loropetalum chinense extract 2%–5%, glycerin 5%, sodium hyaluronate 0.1%–1%, phenoxyethanol 0.1%–1%, disodium EDTA 0.01%–0.05%, citric acid / sodium citrate 0.05%–0.5%, and the balance being deionized water.

[0007] Preferably, the mask fabric is one of silk mask fabric, pure cotton spunlace fabric, viscose spunlace fabric, or bio-fiber fabric.

[0008] On the other hand, this application provides a method for preparing a multi-effect skin care mask containing Loropetalum chinense extract, comprising the following steps: Step 1: Preparation of Loropetalum chinense extract: Under water bath conditions and with ultrasonic-assisted stirring, the dried Loropetalum chinense powder was extracted with ethanol solution for 30 minutes. After filtration, the extract was concentrated under reduced pressure until there was no alcohol odor, and then dried at room temperature to constant weight to obtain Loropetalum chinense extract. Step 2, Aqueous phase preparation: Glycerin, sodium hyaluronate, and disodium EDTA are mixed according to the mass percentage of each component in the mask liquid, and a certain amount of deionized water is added. The mixture is then ultrasonically stirred at 40℃~60℃. Step 3: Active phase dispersion: The Loropetalum chinense extract is pre-dispersed in deionized water at 35-45℃, and most of the pigment is removed by activated carbon. The active phase is obtained by filtration and then slowly added to the aqueous phase and ultrasonically stirred. Step 4: Post-processing of the mask liquid: Add a certain amount of phenoxyethanol to the mixture obtained in step 3, and adjust the pH of the mixture to between 5.0 and 5.5 with citric acid / sodium citrate. Stir ultrasonically until it is uniform and clear, and finally filter it with a microporous membrane to obtain the mask liquid. Step 5: Mask preparation: Place the mask sheet into an aluminum foil bag, add an appropriate amount of mask liquid, and finally vacuum seal it to obtain the mask.

[0009] Preferably, in step one, the water bath temperature is 35–45°C, and the room temperature drying temperature is 20–35°C.

[0010] Preferably, in step one, the ethanol solution is anhydrous ethanol, the ratio of Loropetalum chinense powder to ethanol solution is 1:20 to 1:30 g / mL, and the extract is a clear liquid filtered through fine gauze after centrifugation.

[0011] Preferably, in step three, the concentration of the active phase is 0.2 g / mL, and the amount of active ingredient extract added is 2% to 5% (wt).

[0012] Preferably, in step four, the pore size of the microporous membrane filter is 0.2–0.6 μm.

[0013] The beneficial effects of this invention are: 1. This invention achieves synergistic antioxidant and whitening effects through the scientific formulation of active ingredients such as Loropetalum chinense extract, sodium hyaluronate, and glycerin. The flavonoids and phenolic acids in Loropetalum chinense extract effectively scavenge free radicals, inhibit lipid peroxidation, and reduce melanin production by inhibiting tyrosinase activity. Experiments show that adding 2%–4% of this extract results in an ABTS free radical scavenging rate of 94.99%–100% in the mask liquid and an inhibition rate of tyrosinase as high as 61.49%–81.18%, significantly enhancing the product's antioxidant and whitening efficacy.

[0014] 2. This invention possesses multi-layered moisturizing and skin barrier repair functions. Sodium hyaluronate and glycerin form a complex moisturizing system that can form a water-retaining film on the skin surface, effectively reducing moisture loss and increasing the moisture content of the stratum corneum. Testing has shown that the product can significantly improve dry skin and enhance the skin barrier's resistance.

[0015] 3. The product of this invention exhibits high stability and good safety. By adding EDTA-disodium and phenoxyethanol, metal ions are effectively integrated and microbial growth is inhibited, ensuring the product's stability during storage and use. The citric acid-sodium citrate buffer system keeps the mask solution's pH stable at 5.0-5.5, close to the skin's natural pH, making it gentle and non-irritating, suitable for long-term use.

[0016] 4. This invention expands the application of Loropetalum chinense in the cosmetics field and enhances its resource utilization value. The preparation process is simple, the conditions are mild, and it is easy to scale up production, possessing good industrialization prospects.

[0017] The above description is merely an overview of the technical solution of the present invention. In order to better understand the technical means of the present invention and to implement it in accordance with the contents of the specification, and to make the above and other objects, features and advantages of the present invention more apparent and understandable, specific embodiments of the present invention are described below. Attached Figure Description

[0018] Various other advantages and benefits will become apparent to those skilled in the art upon reading the following detailed description of preferred embodiments. The accompanying drawings are for illustrative purposes only and are not intended to limit the invention. Furthermore, the same reference numerals denote the same parts throughout the drawings. In the drawings: Figure 1 The flowchart illustrates the process of alcohol extraction of Loropetalum chinense extract according to an embodiment of the present invention. Detailed Implementation

[0019] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0020] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0021] Example 1: A multi-effect skin care mask containing Loropetalum chinense extract, comprising a mask liquid and a mask sheet. The mass percentage content of each component in the mask liquid is as follows: Loropetalum chinense extract 2%, glycerin 5%, sodium hyaluronate 0.1%, phenoxyethanol 0.1%, disodium EDTA 0.01%, citric acid / sodium citrate 0.05%, and the balance being deionized water.

[0022] In this embodiment, the preparation method of the multi-effect skin care mask containing Loropetalum chinense extract specifically includes the following steps: Step 1: Preparation of Loropetalum chinense extract: The dried branches and leaves of Loropetalum chinense were crushed, and anhydrous ethanol was added at a ratio of 1:30. The mixture was extracted by ultrasonic stirring in a water bath at 37°C for 30 min. After centrifugation at 5000 rpm, the supernatant was collected, filtered through fine gauze, and concentrated under reduced pressure. The concentrate was then dried in an oven at room temperature until constant weight, which is the Loropetalum chinense extract. Step 2, Aqueous phase preparation: Add 5% glycerol, 0.1% sodium hyaluronate, and 0.01% disodium EDTA by mass percentage, mix them, and add a certain amount of deionized water. Then, ultrasonically stir the mixture at 40°C. Step 3: Active phase dispersion: The Loropetalum chinense extract was pre-dispersed in deionized water at 40℃ to a concentration of 0.2g / mL. Most of the pigments were removed using activated carbon, and the active phase was obtained by filtration. Then, 2% of the active phase was slowly added to the aqueous phase and ultrasonically stirred. Step 4, Post-processing of the mask liquid: Add 0.1% phenoxyethanol to the mixture obtained in step 3, and adjust the pH of the mixture to between 5.0 and 5.5 with 0.05% citric acid / sodium citrate. Stir ultrasonically until uniform and clear, and finally filter with a 0.22μm microporous membrane to obtain the mask liquid. Step 5: Mask preparation: Place the silk mask sheet into an aluminum foil bag, add an appropriate amount of mask liquid, and finally vacuum seal it to obtain the mask.

[0023] Example 2: The multi-effect skin care mask containing Loropetalum chinense extract uses the same raw materials and preparation steps as Example 1. The only difference is the mass percentage content of Loropetalum chinense extract. In this example, the amount of Loropetalum chinense extract added is 4%, and the amount added is determined by the results of antioxidant and whitening activity tests.

[0024] Comparative Example 1 used the same raw materials and preparation steps as Example 1, except that Loropetalum chinense extract was not added in Comparative Example 1.

[0025] Comparative Example 2 used the same raw materials and preparation steps as Example 2, except that the Loropetalum chinense extract component was replaced by Lycopodium clavatum extract. In this comparative example, the amount of Lycopodium clavatum extract added was the same as the amount of Loropetalum chinense extract added.

[0026] The efficacy evaluation of the face mask is as follows: 1. Whitening Efficacy Study—Tyrosinase Activity Inhibition Assay: Using Examples 1 and 2 as test samples, the test substances were dissolved in phosphate-buffered saline (PBS) containing 3.3% DMSO to prepare effector solutions of different concentrations. Using 1 mg / mL levodopa as the substrate and kojic acid as the positive control, different concentrations of effector solutions, PBS buffer, and mushroom tyrosinase were sequentially added to a 200 μL activity assay system in PBS buffer (pH 6.8) to a 96-well plate. The plates were activated at 37°C for 5 min, and finally, the substrate solution was added. The plates were then shaken for 15 min to mix, and the absorbance was measured at 475 nm. Each sample was measured three times, and the inhibition rate was calculated using the formula.

[0027] The formula is: Tyrosinase activity inhibition rate (%) = [1-(A3-A4)] / (A1-A2).

[0028] Table 1. Reaction system for tyrosinase inhibition activity

[0029] Experimental data show that when Loropetalum chinense extract is added to the facial mask liquid at a ratio of 2% (w / w), its inhibition rate against tyrosinase can reach 61.49%; while when the addition amount is increased to 4% (w / w), the inhibition rate significantly increases to 81.18%. This fully demonstrates that the prepared facial mask liquid has excellent whitening effect, and the whitening effect tends to increase with the increase of the amount of Loropetalum chinense extract added. Therefore, the facial mask liquid prepared by the technical solution of this invention has a good whitening effect.

[0030] Table 2 Results of Whitening Activity Test of Facial Mask Liquid

[0031] 2. Antioxidant efficacy study—ABTS free radical scavenging test Using Examples 1 and 2 as test samples, 5 mL of 7.4 mmol / L ABTS solution and an equal volume of 2.6 mmol / L K2S2O8 solution were mixed in a 250 mL beaker. The mixed solution was left to stand in the dark overnight, and deionized water was added to increase its volume by a certain factor so that its absorbance at 734 nm was about 0.70.

[0032] Take the prepared mask solution as the sample solution.

[0033] Mix 10 µL of sample and 190 µL of ABTS working solution in a 96-well plate, incubate at room temperature in the dark for 10 min, and measure the absorbance at 734 nm. Distilled water was used as a blank, and vitamin C was used as a positive control. The ABTS cationic radical scavenging rate was calculated as follows: ABTS cationic radical scavenging rate (%) = 1 - (A1 - A2) / A0 In the formula: A0, absorbance of the sample solution replaced by deionized water; A1, absorbance of the sample solution after mixing with ABTS; A2, absorbance of the sample solution after mixing with ABTS replaced by deionized water.

[0034] The antioxidant activity of the facial mask liquid was quantitatively evaluated using the ABTS free radical scavenging effect. Experimental results revealed that the facial mask liquid exhibited extremely excellent antioxidant properties. Specifically, when the amount of Loropetalum chinense extract added was 2% (w / w), its ABTS free radical scavenging rate reached as high as 94.99%; and when the extract added was increased to 4% (w / w), the scavenging rate reached almost perfect 100%. This result fully highlights the powerful antioxidant effect of Loropetalum chinense extract in the facial mask liquid, which can effectively resist oxidative damage to the skin and delay the skin aging process.

[0035] Table 3 Results of antioxidant activity assay for facial mask liquid

[0036] The functions and effects of each component in the facial mask liquid: 1. Loropetalum chinense extract, mechanism of action: The main active components of this extract are flavonoids (such as quercetin and kaempferol) and phenolic acids (such as gallic acid and ellagic acid). Its antioxidant effect stems from the hydrogen atoms provided by the phenolic hydroxyl groups, which can neutralize free radicals and interrupt the lipid peroxidation chain reaction; its whitening effect is achieved by competitively inhibiting tyrosinase activity, reducing dopaquinone production, and thus inhibiting the melanin synthesis pathway; its anti-inflammatory effect is related to the regulation of the expression of inflammatory factors (such as TNF-α and IL-6).

[0037] Effects: Preclinical studies have shown that this ingredient can significantly reduce DPPH and ABTS free radical levels (scavenging rate >90%) and inhibit tyrosinase activity by more than 60%. Long-term use can improve pigmentation caused by ultraviolet radiation, enhance skin brightness, reduce wrinkle depth, and strengthen the skin barrier function.

[0038] 2. Glycerin, mechanism of action: As a small molecule moisturizer, it binds water molecules through hydrogen bonds to form a moisture-absorbing layer, reducing the evaporation of epidermal moisture; at the same time, it fills the intercellular matrix of the stratum corneum and enhances the orderly arrangement of lipids.

[0039] Effects: It can significantly increase the moisture content of the stratum corneum, relieve dryness and flaking, and improve skin roughness.

[0040] 3. Sodium hyaluronate, mechanism of action: As a high molecular weight polysaccharide, it can form multiple hydrogen bonds with water molecules through its carboxyl and hydroxyl groups, forming a three-dimensional hydration network in the epidermis; at the same time, it binds to CD44 receptors to promote the proliferation and differentiation of keratinocytes.

[0041] Results: Experiments show that it can maintain skin surface moisture for more than 6 hours, reduce transepidermal water loss by about 20%, and enhance skin elasticity.

[0042] 4. Phenoxyethanol, mechanism of action: It disrupts the integrity of microbial cell membranes and inhibits the activity of ATP synthase, leading to impaired intracellular energy metabolism in bacteria.

[0043] Effects: It has a significant inhibitory effect on Gram-positive / negative bacteria and fungi at a concentration of 0.5% to 1.0% (MIC < 500 ppm), which meets the usage restriction requirements of the "Cosmetic Safety Technical Specifications".

[0044] 5. EDTA-disodium, mechanism of action: as a hexadecimal ligand chelating agent, it reacts with Ca... 2+ Mg 2+ Fe 2+ When metal ions form stable complexes, they block the metal ions from participating in oxidation catalysis or microbial enzyme activation processes.

[0045] Effects: It can significantly improve the oxidation stability of products (peroxide value is reduced by 40% in accelerated tests) and enhance the effectiveness of the anti-corrosion system.

[0046] 6. Citric acid-sodium citrate buffer system, mechanism of action: maintains the solution pH in the range of 5.0 to 5.5 through a weak acid-conjugate base system. This pH range is similar to the skin's natural acidic membrane, which can maintain the activity of stratum corneum enzymes and the balance of the microecology.

[0047] Effects: Ensures that active ingredients are effective within the optimal pH range, reducing irritation during use (patch test irritation score ≤0.5).

[0048] 7. Deionized water, mechanism of action: as a polar solvent, it provides a molecular diffusion carrier, promoting the dissolution and transdermal delivery of active ingredients; at the same time, it softens the stratum corneum through hydration.

[0049] Effect: Ensures the uniformity and stability of the formulation system, without affecting the bioavailability of subsequent ingredients.

[0050] The above are merely specific embodiments of the present invention, but the scope of protection of the present invention is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the technical scope disclosed in the present invention should be included within the scope of protection of the present invention. In the absence of conflict, the embodiments and features of the embodiments of the present invention can be combined with each other. Therefore, the scope of protection of the present invention should be determined by the scope of the claims.

Claims

1. A multi-effect skincare mask containing Loropetalum chinense extract, comprising a mask liquid and a mask sheet, characterized in that, The mass percentage content of each component in the mask liquid is as follows: Loropetalum chinense extract 2%–5%, glycerin 5%, sodium hyaluronate 0.1%–1%, phenoxyethanol 0.1%–1%, disodium EDTA 0.01%–0.05%, citric acid / sodium citrate 0.05%–0.5%, and the balance is deionized water.

2. The multi-effect skin care mask containing Loropetalum chinense extract according to claim 1, characterized in that, The mask fabric is one of the following: silk mask fabric, pure cotton spunlace fabric, viscose spunlace fabric, or bio-fiber fabric.

3. The method for preparing the multi-effect skin care mask containing Loropetalum chinense extract according to claim 1, characterized in that, Includes the following steps: Step 1: Preparation of Loropetalum chinense extract: Under water bath conditions and with ultrasonic-assisted stirring, the dried Loropetalum chinense powder was extracted with ethanol solution for 30 minutes. After filtration, the extract was concentrated under reduced pressure until there was no alcohol odor, and then dried at room temperature to constant weight to obtain Loropetalum chinense extract. Step 2, Aqueous phase preparation: Glycerin, sodium hyaluronate, and disodium EDTA are mixed according to the mass percentage of each component in the mask liquid, and a certain amount of deionized water is added. The mixture is then ultrasonically stirred at 40℃~60℃. Step 3: Active phase dispersion: The Loropetalum chinense extract is pre-dispersed in deionized water at 35-45℃, and most of the pigment is removed by activated carbon. The active phase is obtained by filtration and then slowly added to the aqueous phase and ultrasonically stirred. Step 4: Post-processing of the mask liquid: Add a certain amount of phenoxyethanol to the mixture obtained in step 3, and adjust the pH of the mixture to between 5.0 and 5.5 with citric acid / sodium citrate. Stir ultrasonically until it is uniform and clear, and finally filter it with a microporous membrane to obtain the mask liquid. Step 5: Mask preparation: Place the mask sheet into an aluminum foil bag, add an appropriate amount of mask liquid, and finally vacuum seal it to obtain the mask.

4. The method for preparing the multi-effect skin care mask containing Loropetalum chinense extract according to claim 3, characterized in that, In step one, the water bath temperature is 35-45℃, and the room temperature drying temperature is 20-35℃.

5. The method for preparing the multi-effect skin care mask containing Loropetalum chinense extract according to claim 4, characterized in that, In step one, the ethanol solution is anhydrous ethanol, the ratio of Loropetalum chinense powder to ethanol solution is 1:20 to 1:30 g / mL, and the extract is a clear liquid filtered through fine gauze after centrifugation.

6. The method for preparing the multi-effect skin care mask containing Loropetalum chinense extract according to claim 4, characterized in that, In step three, the concentration of the active phase is 0.2 g / mL, and the amount of active ingredient extract added is 2% to 5% (wt).

7. The method for preparing the multi-effect skin care mask containing Loropetalum chinense extract according to claim 6, characterized in that, In step four, the pore size of the microporous membrane is 0.2–0.6 μm.

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