Composite plant composition for synchronizing and amplifying skin circadian rhythm and preparation method

Abstract

The invention discloses a composite plant composition for synchronizing and magnifying skin circadian rhythm, which comprises a myrothamnus flabellifolia leaf / stem extract, a crithmum maritimum extract, a Thermus thermophilus fermentation product and a broad-leaved epiphyllum callus extract in a weight ratio of (1-3): (0.1-0.5): (0.5-4): (0.5-10). The myrothamnus flabellifolia leaf / stem extract, the crithmum maritimum extract, the Thermus thermophilus fermentation product and the broad-leaved epiphyllum callus extract are combined to act, so that the disordered skin biological clock can be reset, and the time rhythm can be synchronized. And on the basis of rhythm synchronization, the nighttime repair capability of the skin can be remarkably amplified, DNA damage repair is accelerated, barrier related protein synthesis is promoted, and the cell renewal rate is increased.

Description

Technical Field

[0001] This invention relates to the field of cosmetic formulations, specifically to a composite plant composition and its preparation method that synchronizes and amplifies the skin's circadian rhythm, as well as its application and methods for preparing cosmetics. Background Technology

[0002] Skin cell physiological activities are precisely regulated by an intrinsic circadian rhythm. Core repair functions, such as DNA repair and cell renewal, occur at night, driven by the oscillations in the expression of circadian clock genes (such as CLOCK, BMAL1, Per, and Cry). Modern lifestyles, such as staying up late and exposure to blue light from mobile phones and computers, disrupt the expression of these circadian clock genes, affecting nighttime skin repair and leading to a tired appearance, dull skin, and weakened barrier function the next day. Currently, hormonal products like melatonin are used to simulate systemic signals, but they cannot "reset" the skin cell's own circadian rhythm. In the cosmetics industry, repair-focused night creams only provide passive nutrient replenishment such as moisturizing and anti-oxidation, failing to target the disordered circadian clock genes and fundamentally "calibrate" the timing and intensity of repair. Simple combinations of plant extracts often lack a rational design targeting the synergistic pathway of "circadian rhythm synchronization" and "repair amplification," merely piling up ingredients with limited effects. Therefore, developing a natural complex that can actively and precisely synchronize disordered skin rhythms and effectively amplify subsequent repair signals is crucial.

[0003] Chinese invention patent CN119139205B discloses a skincare composition with repairing and anti-aging effects, comprising sea fennel extract and Myrothamnus flabellifolia leaf / stem extract. It has repairing and anti-aging effects and can be used to prepare sebum-inspired cosmetics. However, it does not involve the rational design of the synergistic pathway of "rhythm synchronization" and "repair amplification," but is merely a piling up of ingredients. Chinese invention patent application CN120617138A discloses a tea callus tissue composition with repairing and anti-wrinkle effects and its application, comprising tea callus tissue extract, snow lotus callus tissue extract, and epiphyllum callus tissue extract. It has excellent repairing and anti-wrinkle effects, but does not fundamentally synchronize the disordered skin rhythm. Summary of the Invention

[0004] In order to develop a compound plant composition that can precisely regulate the expression of circadian rhythm genes in skin cells through multi-target synergistic action and enhance the subsequent repair process, the first aspect of the present invention provides a compound plant composition for synchronizing and amplifying the skin's diurnal rhythm, comprising Myrothamnus flabellifolia leaf / stem extract, sea fennel extract, thermophilic bacteria fermentation product and Epiphyllum callus extract, wherein the weight ratio of Myrothamnus flabellifolia leaf / stem extract, sea fennel extract, thermophilic bacteria fermentation product and Epiphyllum callus extract is (1-3):(0.1-0.5):(0.5-4):(0.5-10).

[0005] In one embodiment, the weight ratio of the Myrothamnus fruticosa leaf / stem extract, sea fennel extract, thermophilic bacteria fermentation product and Epiphyllum callus extract is (1-2):(0.2-0.4):(0.5-1):(1-10).

[0006] In one embodiment, the weight ratio of the Myrothamnus flabellifolia leaf / stem extract, sea fennel extract, thermophilic bacteria fermentation product and Epiphyllum callus extract is 1:0.3:0.5:1.

[0007] In one implementation, the Myrothamnus flabellifolia leaf / stem extract has a rhythm synchronizer effect, the Crithmetic cinnamomea extract and Thermus thermophilus fermentation product together act as rhythm amplifiers, and the Epiphyllum oxypetalum callus extract achieves a synergistic enhancement effect. The synergistic effect of the compound plant composition can reset disordered skin biological clocks and synchronize time rhythms, achieving the synergistic effect of "rhythm synchronization" and "repair amplification".

[0008] A second aspect of the present invention provides a method for preparing a complex plant composition for synchronizing and amplifying the skin's diurnal rhythm, comprising the following steps: Under conditions of <45℃, extracts of Myrothamnus fruticosa leaves / stems, extracts of sea fennel, fermentation products of Thermophilus membranaceus, and extracts of Epiphyllum callus were mixed and stirred evenly to obtain a compound plant composition.

[0009] As one implementation, when the compound plant composition is used in cosmetics, the extracts of Myrothamnus flabellifolia leaves / stems, sea fennel extract, thermophilic bacteria fermentation product, and Epiphyllum callus extract are dissolved in deionized water for use.

[0010] A third aspect of the invention provides an application of a complex plant composition for synchronizing and amplifying the skin’s circadian rhythm in a skincare product, wherein the complex plant composition is added to the skincare product in an amount of 3-10 wt%.

[0011] As one embodiment, the raw materials for preparing the skin care product include, by weight percentage, 10-20% phase B, 0.1-5% phase C, 0.1-1% phase D, and phase A to 100%; phase A includes the above-mentioned compound plant composition for synchronizing and amplifying the skin's diurnal rhythm, phase B is an oil phase, phase C is a pH adjuster, and phase D is a preservative.

[0012] In one embodiment, phase A comprises, by weight percentage of the skincare product: 3-10% of the above-mentioned compound plant composition for synchronizing and amplifying the skin's circadian rhythm, 0.1-5% of a thickener, 1-5% of glycerin, and deionized water to bring the total to 100%.

[0013] In one embodiment, phase B comprises, by weight percentage of the skincare product, 1-5% emulsifier and 10-20% emollient.

[0014] In one embodiment, the emulsifier is cetearyl oleate or sorbitan oleate.

[0015] In one embodiment, the emollient comprises caprylic / capric triglyceride, isononyl isononanoate, and shea butter, wherein the weight ratio of caprylic / capric triglyceride, isononyl isononanoate, and shea butter is (5-10):(3-8):(1-3).

[0016] In one embodiment, the thickener is carbomer Ultraz 21.

[0017] In one embodiment, the pH adjuster is triethanolamine.

[0018] In one embodiment, the preservative is phenoxyethanol or ethylhexylglycerin.

[0019] A fourth aspect of the present invention provides a method for preparing a skincare product using a complex plant composition for synchronizing and amplifying the skin's diurnal rhythm, comprising the following steps: Heat deionized water and glycerin to 75-80℃, disperse the thickener, and homogenize to obtain phase A premix. Mix the raw materials of phase B and heat to 75-80℃ until completely dissolved; Slowly add the completely dissolved B phase to the A phase premix and homogenize for 3-5 minutes to form an emulsion; Cool the emulsion to below 45°C, and add phase C to adjust the pH to 6.0-6.5; Continue cooling to below 40℃, add the compound plant composition and phase D, and stir until homogeneous; Continue stirring and cooling to room temperature to obtain a skincare product containing a complex plant composition for synchronizing and amplifying the skin's circadian rhythm.

[0020] Compared with the prior art, the present invention has the following beneficial effects: (1) The compound plant composition for synchronizing and amplifying the skin’s diurnal rhythm described in this invention combines Myrothamnus flabellifolia leaf / stem extract, sea fennel extract, thermophilic bacteria fermentation product and Epiphyllum callus extract to reset disordered skin biological clock and synchronize time rhythm.

[0021] (2) The compound plant composition of the present invention for synchronizing and amplifying the skin’s circadian rhythm can significantly amplify the skin’s own nighttime repair ability on the basis of rhythm synchronization, accelerate DNA damage repair, promote the synthesis of barrier-related proteins, and improve cell renewal rate.

[0022] (3) The composite plant composition described in this invention for synchronizing and amplifying the skin’s circadian rhythm can be applied to cosmetics to improve skin problems caused by rhythm disorder. After use, it can quickly improve dullness, fatigue and roughness of the skin after staying up late, and restore the skin to a healthy glow.

[0023] (4) The compound plant composition of the present invention for synchronizing and amplifying the skin’s diurnal rhythm can actively and precisely synchronize disordered skin rhythms through multi-target synergistic effects, regulate the expression of biological clock genes in skin cells, and effectively amplify their subsequent repair signals.

[0024] (5) The composite plant composition of the present invention for synchronizing and amplifying the skin’s diurnal rhythm, when applied in cosmetics, gives the skin stronger stress resistance and improves the skin’s ability to defend against environmental stresses such as blue light and pollution during the day by optimizing the rhythm. Detailed Implementation

[0025] Example A complex plant composition for synchronizing and amplifying the skin’s circadian rhythm, comprising Myrothamnus flabellifolia leaf / stem extract, sea fennel extract, thermophilic bacteria fermentation product and Epiphyllum callus extract in a weight ratio of 1:0.3:0.5:1.

[0026] The Myrothamnus flabellifolia leaf / stem extract was purchased from RAHN under the name MYRAMAZE® -ESSENCE and is an oily solution; the sea fennel extract was purchased from CODIF under the name AREAUMATS and is a powder; the thermophilic bacteria fermentation product was purchased from Guangzhou Tenus Chemical under the name thermophilic bacteria and is a transparent solution; and the Epiphyllum callus extract was purchased from Hangzhou Weiming Times under the name VEMERSTEM NightElf and is a transparent solution.

[0027] A method for preparing a complex plant composition for synchronizing and amplifying the skin's diurnal rhythm includes the following steps: Under conditions of <45℃, extracts of Myrothamnus fruticosa leaves / stems, extracts of sea fennel, fermentation products of Thermophilus membranaceus, and extracts of Epiphyllum callus were mixed and stirred evenly to obtain a compound plant composition.

[0028] Application of a complex plant composition for synchronizing and amplifying the skin's circadian rhythm in a skincare product, wherein the complex plant composition is added to the skincare product at an amount of 5 wt%.

[0029] The raw materials for preparing the skincare product include, by weight percentage, 18% phase B, 0.15% phase C, 0.6% phase D, and phase A to make up to 100%; phase A includes the above-mentioned compound plant composition for synchronizing and amplifying the skin's circadian rhythm, phase B is an oil phase, phase C is a pH adjuster, and phase D is a preservative.

[0030] The above-mentioned compound plant composition for synchronizing and amplifying the skin's circadian rhythm is 5%, thickener 0.15%, glycerin 5%, and deionized water is added to make 100%.

[0031] Phase B, by weight percentage of the skincare product, comprises: 3% emulsifier and 15% emollient.

[0032] The emulsifier is cetearyl oleate.

[0033] The emollient comprises caprylic / capric triglyceride, isononyl isononanoate, and shea butter, wherein the weight ratio of caprylic / capric triglyceride, isononyl isononanoate, and shea butter is 8:5:2.

[0034] The thickener is carbomer Ultraz 21. The pH adjuster is triethanolamine. The preservative is phenoxyethanol.

[0035] A method for preparing a skincare product using a complex plant composition for synchronizing and amplifying the skin's circadian rhythm, comprising the following steps: Heat deionized water and glycerin to 75-80℃, disperse the thickener, and homogenize to obtain phase A premix. Mix the raw materials of phase B and heat to 75-80℃ until completely dissolved; Slowly add the completely dissolved B phase to the A phase premix, homogenize for 4 minutes to form an emulsion; Cool the emulsion to below 45°C, and add phase C to adjust the pH to 6.0-6.5; Continue cooling to below 40℃, add the compound plant composition and phase D, and stir until homogeneous; Continue stirring and cooling to room temperature to obtain a skincare product containing a complex plant composition for synchronizing and amplifying the skin's circadian rhythm.

[0036] Comparative Example 1 A complex plant composition for synchronizing and amplifying the skin's circadian rhythm and its preparation method are disclosed, along with its application in cosmetic preparation methods. The specific implementation method is the same as the previous example, except that the complex plant composition for synchronizing and amplifying the skin's circadian rhythm includes Myrothamnus flabellifolia leaf / stem extract and Epiphyllum oxypetalum extract in a weight ratio of 1:1.

[0037] The epiphyllum extract was prepared by the following conventional water-alcohol extraction method: Weigh 1 part by weight of the coarse powder of the aerial parts of Epiphyllum oxypetalum, add 8 parts by weight of 60 vol% ethanol aqueous solution, and heat and reflux at 65℃ for 2 h.

[0038] Filtration and secondary extraction: Filter while hot and collect the filtrate; add 6 parts by weight of 60 vol% ethanol solution to the filter residue and repeat the extraction once under the same conditions, then combine the two filtrates.

[0039] The combined filtrates were concentrated at 55°C under vacuum until all ethanol was removed, yielding a concentrated aqueous solution.

[0040] Deionized water was added to the concentrate and the volume was adjusted to 1 liter to obtain a 1:1 (g / mL) extract of Epiphyllum oxypetalum.

[0041] Comparative Example 2 A complex plant composition for synchronizing and amplifying the skin's circadian rhythm and its preparation method are disclosed, along with its application in cosmetic preparation methods. The specific implementation method is the same as the previous examples, except that the complex plant composition for synchronizing and amplifying the skin's circadian rhythm comprises only sea fennel extract.

[0042] Comparative Example 3 A compound plant composition for synchronizing and amplifying the skin's circadian rhythm and its preparation method are disclosed, along with its application in cosmetic preparation methods. The specific implementation method is the same as the previous example, except that the compound plant composition for synchronizing and amplifying the skin's circadian rhythm includes Myrothamnus flabellifolia leaf / stem extract, sea fennel extract, and thermophilic bacteria fermentation product in a weight ratio of 1:0.3:0.5.

[0043] Comparative Example 4 A complex plant composition for synchronizing and amplifying the skin's circadian rhythm and its preparation method are disclosed, along with its application in cosmetic preparation methods. The specific implementation method is the same as the previous example, except that the complex plant composition for synchronizing and amplifying the skin's circadian rhythm includes Myrothamnus flabellifolia leaf / stem extract, sea fennel extract, thermophilic bacteria fermentation product, and Epiphyllum oxypetalum extract, in a weight ratio of 1:0.3:0.5:1.

[0044] Performance testing I. Evaluation method for multi-target synchronization efficiency of in vitro biological clock genes: A human dermal fibroblast model carrying dual reporter genes Bmal1-dLuc (reporter rhythm cycle) and Per2-dLuc (reporter rhythm phase) was constructed using CRISPR / Cas9 technology. After induced rhythm disturbance by dexamethasone shock therapy, cells were treated with culture media containing 1 wt% of the example and 1 wt% of each comparative example, respectively, of a composite plant composition. Real-time fluorescence microscopy was used to continuously monitor the rhythm cycle, amplitude, and phase shift for 96 hours, and the data were analyzed. The test results are shown in Table 1.

[0045] The normal control group consisted of healthy cells that had not been induced with dexamethasone and had not been given any test samples. The model group consisted of rhythm disordered cells induced with dexamethasone but without any test samples.

[0046] Table 1

[0047] The embodiments of this invention exhibit optimal effects in three key dimensions: periodic recovery, amplitude enhancement, and phase correction, almost completely restoring disordered rhythms to normal. This indicates that its mechanism of action is multi-target and systemic. Comparative Examples 3 and 4 show incomplete effects, lacking key synergistic enhancing components, demonstrating that the Epiphyllum callus extract makes a unique contribution to enhancing rhythmic amplitude and stabilizing the period.

[0048] II. Integrated Nighttime Repair Efficacy Assessment (Verification of "Repair Amplification" Effect) 1. Experimental Objective: Based on successful synchronization of cell rhythms, to quantitatively evaluate the amplification effect of the complex of this invention on a series of downstream repair pathways (including DNA repair, cell proliferation, extracellular matrix synthesis and cellular energy metabolism) during the cell's own "biological night" period (i.e. the period when repair functions are most active).

[0049] 2. The experimental model is synchronized with the rhythm. Cell model: human dermal fibroblasts (HDF), derived from neonatal foreskin or commercial cell lines (such as HS27).

[0050] Circadian rhythm synchronization: To avoid the long-term effects of dexamethasone, a more physiological method was used. After the cells were seeded and grown to 80% confluence, the medium was replaced with one containing 50 vol% horse serum and treated for 2 hours to induce endogenous circadian rhythm synchronization. Subsequently, the medium was replaced with normal complete medium (containing 10 vol% fetal bovine serum).

[0051] Definition and sample loading of the "bionight" period: The period from 12 to 24 hours after serum shock is defined as the "bionight" repair window for cells. At 12 hours after serum shock, the old culture medium was discarded and replaced with complete culture medium containing 1% (v / v) of the samples from Examples 1-4, and normal culture medium without samples (model group). Cells were cultured under these conditions for another 12 hours (i.e., the entire "bionight") to assess the amplification effect of the samples on repair function.

[0052] 3. Detection Indicators and Specific Methods 3.1. DNA repair capacity assessment (8-OHdG clearance rate) Principle: Hydrogen peroxide (H2O2) is used to induce oxidative damage to cellular DNA in vitro, producing 8-hydroxydeoxyguanosine (8-OHdG). The DNA repair efficacy is evaluated by detecting the ability of cells to scavenge 8-OHdG after sample treatment.

[0053] Specific sample addition and testing steps: Damage induction: After the above "biological night" treatment, the culture medium was discarded. All groups (including the model group) were added with PBS solution containing 200 μM H2O2 and incubated at 37°C for 30 minutes to induce DNA oxidative damage.

[0054] Repair phase: Discard the H2O2 solution and wash the cells twice with PBS.

[0055] The cells were redivided into two parts: Part A (Repair Detection): Refill the corresponding culture medium containing 1v / v% of the sample (Example and Comparative Groups) or normal culture medium (Model Group), and continue culturing for 2 hours to allow the cells to repair.

[0056] Part B (damage baseline): Cell lysis buffer was added, and cells were immediately lysed. This was taken as the maximum damage value at time 0.

[0057] Sample collection: Two hours after remedy, cells in part A were also lysed using cell lysis buffer.

[0058] Detection: Using an 8-OHdG ELISA kit (e.g., Japan Institute for the Control of Aging product), strictly follow the instructions to detect the 8-OHdG content in all cell lysates.

[0059] Results calculation: 8-OHdG scavenging rate (%) = [1 - (8-OHdG content in the sample after remedy / 8-OHdG content in the model group after remedy)] × 100% 3.2. Assessment of cell proliferation viability Principle: Using CCK-8 reagent, the amount of yellow formazan dye produced by it under the action of mitochondrial dehydrogenase in living cells is directly proportional to the number of living cells.

[0060] Specific sample addition and testing steps: After the "biological night" treatment is completed, the test is carried out directly.

[0061] Add CCK-8 solution (10% of the culture medium volume) to each well.

[0062] Place the cell culture plate in a 37°C incubator and incubate in the dark for 1-4 hours.

[0063] The absorbance (OD value) of each well was measured using an ELISA reader at a wavelength of 450 nm.

[0064] The results indicate that relative cell proliferation activity = (OD value of sample group / OD value of model group) × 100% 3.3. Assessment of Type I Collagen (COL1A1) Secretion Principle: The content of COL1A1 in cell culture supernatant is specifically detected by enzyme-linked immunosorbent assay (ELISA).

[0065] Specific sample addition and testing steps: Sample collection: After the “biological night” treatment is completed, carefully collect the cell culture supernatant from each group.

[0066] Assay: Use a human type I collagen propeptide ELISA kit (e.g., R&D Systems, USA) and strictly follow the instructions. Add the supernatant to a pre-coated antibody-labeled plate, then add the biotinylated detection antibody, streptomycin-HRP, and substrate solution sequentially. Finally, read the value at 450 nm.

[0067] Standard curve: Plot the standard curve using the COL1A1 standard provided in the kit.

[0068] The results indicate that the relative COL1A1 secretion level = (COL1A1 concentration in the sample group / COL1A1 concentration in the model group) × 100%. 3.4. Assessment of Intracellular ATP Levels Principle: ATP is the direct energy currency of cells, and its level directly reflects the energy metabolism state of cells.

[0069] Specific sample addition and testing steps: Sample collection: After the "biological night" treatment, the culture medium was discarded and the cells were washed with PBS.

[0070] Cell lysis: Add an appropriate amount of ATP to detect the lysis buffer and lyse the cells.

[0071] Assay: Use an ATP assay kit (e.g., Beyotime product). Mix the lysis buffer and ATP assay working solution in a white opaque 96-well plate at the specified ratio.

[0072] The chemiluminescence signal (RLU value) is detected using a chemiluminescence analyzer or a fluorescence microplate reader.

[0073] The results are expressed as: Relative ATP level = (RLU value of sample group / RLU value of model group) × 100% In a circadian rhythm-synchronized fibroblast model (during the "biological night" period), a multi-indicator joint detection method was used: The test results are shown in Table 2.

[0074] Table 2

[0075] The examples demonstrate a comprehensive amplification effect on repair. The effect far exceeds the simple sum of the effects of the individual components (Comparative Examples 2 and 4), proving a clear synergistic effect. The repair effect of Comparative Example 3 is significantly lower than that of the examples, demonstrating that the Epiphyllum callus extract is a key "synergist," crucial for improving overall repair efficiency.

[0076] 3. Evaluation of clinical efficacy in humans Sixty qualified volunteers were recruited and randomly assigned to six groups (n=10). A randomized, double-blind, placebo-controlled experimental design was employed. The Example Groups used a night cream containing 5 wt% of the complex from Example 1 of this invention; Comparative Examples 1-4 used a night cream containing 5% of the corresponding comparative example composition; and the placebo Group used a blank base night cream without any active composition. All volunteers used the cream once nightly for eight weeks.

[0077] Evaluation metrics and baseline: Skin tone luminance (ITA°) and transepidermal water loss (TEWL) values ​​of volunteers' faces were measured under standard experimental conditions before use (day 0 baseline) and 8 weeks after use. The ITA° improvement value was the difference between the value after 8 weeks and the baseline value; the percentage reduction in TEWL = (baseline value - value after 8 weeks) / baseline value × 100%.

[0078] Table 3: Changes in efficacy indicators after 8 weeks of use (±SD compared to baseline) The test results are shown in Table 3.

[0079] Table 3

[0080] The results of the human trials were highly consistent with those of the in vitro experiments. The control group demonstrated significantly better results than all comparative groups in improving dull skin tone and strengthening the skin barrier. Comparative examples 3 and 4 showed effects between the control group and comparative example 1, further confirming the necessity of the Epiphyllum callus extract and the Myrothamnus flabellifolia leaf / stem extract.

Claims

1. A complex plant composition for synchronizing and amplifying the circadian rhythm of the skin, characterized in that, The weight ratio of the Myrothamnus leaf / stem extract, the Enchylaena extract, the Thermus thermophilus fermentation product and the Epiphyllum inermum callus extract is (1-3): (0.1-0.5): (0.5-4): (0.5-10).

2. The compound botanical composition for synchronizing and amplifying the skin circadian rhythm according to claim 1, wherein The weight ratio of the Myrothamnus leaf / stem extract, the Enchylaena extract, the Thermus thermophilus fermentation product and the Epiphyllum inermum callus extract is (1-2): (0.2-0.4): (0.5-1): (1-10).

3. The compound botanical composition for synchronizing and amplifying the skin circadian rhythm according to claim 2, wherein The weight ratio of the Myrothamnus leaf / stem extract, the Enchylaena extract, the Thermus thermophilus fermentation product and the Epiphyllum inermum callus extract is 1:0.3:0.5:

1.

4. A method of preparing a complex plant composition for synchronizing and amplifying the circadian rhythm of the skin according to any one of claims 1-3, characterized in that, The method comprises the following steps: The Myrothamnus leaf / stem extract, the Enchylaena extract, the Thermus thermophilus fermentation product and the Epiphyllum inermum callus extract are mixed at a temperature lower than 45 DEG C, and stirred uniformly to obtain a composite plant composition.

5. Use of a complex phytocomplex for synchronizing and amplifying the circadian rhythm of the skin according to any one of claims 1-3, characterized in that, The composite plant composition is applied to a skin care product, and the amount of the composite plant composition added to the skin care product is 3-10 wt%.

6. Use of the complex botanical composition for synchronizing and amplifying the circadian rhythm of the skin according to claim 5, characterized in that, The preparation raw materials of the skin care product comprise, in terms of weight percentage, 10-20% of B phase, 0.1-5% of C phase, 0.1-1% of D phase, and A phase supplementing to 100%; the A phase comprises the composite plant composition for synchronizing and amplifying the circadian rhythm of skin according to any one of claims 1-3, the B phase is an oil phase, the C phase is a pH regulator, and the D phase is a preservative.

7. Use of a complex botanical composition for synchronizing and amplifying the circadian rhythm of the skin according to claim 6, characterized in that, The A phase comprises, in terms of weight percentage of the skin care product, 3-10% of the composite plant composition for synchronizing and amplifying the circadian rhythm of skin according to any one of claims 1-3, 0.1-5% of a thickening agent, 1-5% of glycerol, and deionized water supplementing to 100%.

8. Use of the complex botanical composition for synchronizing and amplifying the circadian rhythm of the skin according to claim 6, characterized in that, The B phase comprises, in terms of weight percentage of the skin care product, 1-5% of an emulsifier and 10-20% of an emollient.

9. Use of a complex botanical composition for synchronizing and amplifying the circadian rhythm of the skin according to claim 8, characterized in that, The emollient comprises caprylic / capric triglyceride, isononyl isononanoate and shea butter, and the weight ratio of the caprylic / capric triglyceride, the isononyl isononanoate and the shea butter is (5-10):(3-8):(1-3).

10. A method for preparing a skin care product using the composite plant composition for synchronizing and amplifying the skin circadian rhythm according to any one of claims 7-9, characterized in that, The method comprises the following steps: The deionized water and the glycerol are heated to 75-80 DEG C, the thickening agent is dispersed, and the A phase premix is uniformly homogenized; The raw materials of the B phase are mixed, heated to 75-80 DEG C, and completely dissolved; The completely dissolved B phase is slowly added to the A phase premix, homogenized for 3-5 min to form an emulsion; The emulsion is cooled to a temperature lower than 45 DEG C, the C phase is added to adjust the pH to 6.0-6.5; The cooling is continuously performed to a temperature lower than 40 DEG C, the composite plant composition and the D phase are added, and stirred uniformly; The stirring is continuously performed to cool to room temperature to obtain the skin care product containing the composite plant composition for synchronizing and amplifying the circadian rhythm of skin.

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