Composition for preventing and improving skin aging and cosmetic containing same

Abstract

The invention relates to a composition for preventing and improving skin aging and a cosmetic containing the same. The composition comprises a red rice extract and PDRN. The composition disclosed by the invention has a synergistic effect on promoting the generation of type I collagen, so that skin aging can be remarkably prevented and / or improved.

Description

TECHNICAL FIELD

[0001] The present application relates to a composition for preventing and improving skin aging and a cosmetic containing the same. BACKGROUND

[0002] Skin aging mainly includes intrinsic aging and extrinsic aging. Extrinsic aging refers to the aging changes caused by the influence of environmental factors on the skin, and is mainly affected by ultraviolet (UV) radiation, so it is also called photoaging. Both long-wave ultraviolet (UVA) and medium-wave ultraviolet (UVB) can cause photoaging. Because the burning effect of UVB on the skin is 1000 times stronger than that of UVA, UVB has been considered as the main spectrum leading to photoaging for a long time. However, recent studies have shown that UVA is the main spectrum causing skin photoaging.

[0003] The main clinical manifestations of skin photoaging are skin wrinkles, roughness, dryness, laxity, telangiectasia, increased fragility, and pigmentation. Long-term sunlight exposure can affect the skin and change the structure of various cellular components and tissues, among which the most characteristic change is the change of the extracellular matrix components in the dermis. The extracellular matrix of the dermis includes collagen fibers, elastic fibers, amino polysaccharides, and protein polysaccharides, etc., among which collagen type I is the main structural protein. Studies have shown that the decrease in the content of collagen in the dermis layer and the destruction of the structure are one of the important reasons leading to skin aging. Collagen type I is the main biochemical component of collagen fibers, which is secreted by fibroblasts, polymerized into collagen fibrils outside the cells, and then a small amount of collagen fibers are formed. As one of the main components of the dermis of the skin, the content of collagen fibers is directly related to the aging characteristics such as skin wrinkles and laxity.

[0004] Therefore, preventing the degradation of collagen and matrix molecules and promoting the synthesis of collagen and matrix become the key to the development of anti-aging skin care products.

[0005] Polydeoxyribonucleotides (PDRN) are a mixture of deoxyribonucleotides with different molecular weights, which are derived from salmon or trout sperm DNA, and the DNA fragments are most similar to the human body. PDRN is used for cosmetic injection, which can activate the self-healing ability of the skin, stimulate the continuous generation of growth factors inside the skin, and promote the activity of fibroblasts.

[0006] It is reported that red rice extract contains phenolic compounds such as anthocyanins and proanthocyanidins, and red rice extract can effectively resist ROS (reactive oxygen species) induced by blue light, inhibit melanin production and collagen degradation, and is an effective representative of biological protection ingredients.

[0007] It is still a technology to be developed to seek a more efficient product for promoting collagen production. SUMMARY

[0008] In view of the above-mentioned defects of the prior art, the present application provides a composition for preventing and improving skin aging and a cosmetic containing the same. The composition of the present application has a synergistic effect on promoting the production of type I collagen, thereby being capable of significantly preventing and / or improving skin aging.

[0009] In one aspect of the present application, there is provided a composition for preventing and / or improving skin aging, comprising red rice extract and PDRN.

[0010] In the present application, the skin aging can be caused by light, preferably light in the UVA band. The UVA band generally refers to 320-400 nm. It has strong penetrating power, and UVA can directly reach the dermis layer of the skin, damage elastic fibers and collagen fibers, and cause skin aging.

[0011] In the present application, the red rice extract preferably comprises phenolic compounds. Among them, the phenolic compounds preferably comprise polyphenolic compounds and / or phenolic acid compounds. Among them, the polyphenolic compounds are the main active ingredients. Among them, those skilled in the art know that the polyphenolic compounds generally comprise anthocyanins and / or proanthocyanidins.

[0012] Among them, the polyphenolic compounds in the red rice extract preferably account for more than 6%, for example 10%, 20%, 25%, 30%, 40% or 50% of the total mass of the red rice extract, more preferably 20%-30% of the total mass of the red rice extract. In a specific embodiment of the present application, according to the polyphenol content of 1.83 mg / mL in the plant stock solution, it is calculated that the polyphenolic compounds account for 26.1% of the total mass of the red rice extract.

[0013] In the present application, the preparation method of the red rice extract preferably comprises the following steps: after the red rice is extracted with an aqueous alcohol solution, the supernatant is collected by solid-liquid separation; the substance after removing the solvent from the supernatant is the red rice extract; the alcohol can include one or more of monohydric alcohol, dihydric alcohol and trihydric alcohol. Among them, those skilled in the art can expect that the substance after removing the solvent from the supernatant is usually a solid.

[0014] Among them, the red rice is preferably red rice bran. The D50 particle size of the red rice bran is preferably 20-100 microns.

[0015] Among them, the monohydric alcohol can be an alkyl monohydric alcohol, preferably ethanol.

[0016] Among them, the dihydric alcohol can be an alkyl dihydric alcohol, preferably propylene glycol and / or butanediol; wherein the propylene glycol is preferably 1,2-propylene glycol.

[0017] Preferably, the mass ratio of the red rice to the aqueous alcohol solution is 1:(4-19), for example 1:5 or 1:6.

[0018] Preferably, the mass concentration of alcohol in the aqueous alcohol solution is 30-80%, for example 40%, 50%, 60% or 70%.

[0019] Preferably, the temperature of the extraction is 35-55°C.

[0020] Preferably, the time of the extraction is 8-13.0 hours.

[0021] Preferably, the extraction is by stirring. The stirring speed is preferably 150-200 rpm.

[0022] Preferably, the solid-liquid separation is by centrifugation.

[0023] Preferably, the supernatant is a supernatant after membrane filtration, which is performed to remove residual particulate matter. The membrane filter size can be conventional in the art, and is generally 0.22-8.00 μm, preferably 0.50 μm.

[0024] Preferably, the mass content of the red rice extract in the supernatant is 0.5% or more, more preferably 0.5-1%, for example 0.7%. Those skilled in the art will appreciate that the mass content of the red rice extract can be expressed as the solid content in the supernatant.

[0025] Preferably, the PDRN has a molecular weight of 50-60 KDa.

[0026] Preferably, the mass ratio of the red rice extract to the PDRN is 1:(0.1-1), for example 1:0.400, 1:0.420, 1:0.500, 1:0.600, 1:0.700, 1:0.714, 1:0.800 or 1:0.900.

[0027] Preferably, the composition is in the form of a solution.

[0028] Preferably, the composition contains a solvent, which generally includes water. Alternatively, to increase the solubility of the red rice extract and / or the PDRN in water, the solvent can further include an alkyl dihydric alcohol and / or an alkyl monohydric alcohol. Preferably, the solvent includes one or more of ethanol, 1,2-propanediol, butylene glycol and 1,2-pentanediol. The amount of the alkyl dihydric alcohol added can be selected as required, provided that the red rice extract and the PDRN are dissolved, without particular limitation.

[0029] In the present application, the concentration of the red rice extract in the composition is preferably 4 ppm or more, more preferably 4-50 ppm, such as 4.375 ppm, 8.75 ppm, 17.5 ppm, 35 ppm or 50 ppm. Those skilled in the art can understand from the examples that when the composition is applied to cosmetics, the concentration of the red rice extract in the cosmetics is preferably also 4 ppm or more.

[0030] In the present application, the concentration of the PDRN is preferably 1 ppm or more, preferably 1-30 ppm, such as 1.875 ppm, 3 ppm, 3.75 ppm, 6.25 ppm, 7.5 ppm, 12.5 ppm, 15 ppm, 25 ppm or 30 ppm. Those skilled in the art can understand from the examples that when the composition is applied to cosmetics, the concentration of the PDRN in the cosmetics is preferably also 1 ppm or more.

[0031] Those skilled in the art know that in a dilute solution: when the solution density is close to 1 g / mL, 1 ppm ≈ 1 mg / L. In the present application, the density of each solution is close to 1 g / mL.

[0032] In another aspect of the present application, a composition for promoting the production of type I collagen is provided, which comprises the red rice extract and the PDRN.

[0033] In another aspect of the present application, a cosmetic comprising the composition is also provided.

[0034] In the present application, the cosmetic can have the meaning commonly understood in the art, generally referring to a product applied to the human skin by smearing, spraying, alcohol or other similar methods, for the purpose of cleaning, maintaining, beautifying, modifying and changing the appearance to maintain a good state. In the present application, the dosage form of the cosmetic can be conventional in the art, including but not limited to cream, emulsion, aerosol, water or gel. The cosmetic of the present application can be a makeup cosmetic, a base cosmetic, a skin care product or a sunscreen cosmetic, and further the types of the cosmetic include but are not limited to foundation, sunscreen, sunscreen emulsion, sunscreen spray, hand cream, face cream, toner, sunscreen cream, mask or serum.

[0035] Positive progress effect: the composition containing red rice extract and PDRN in the present application has a synergistic effect on promoting the production of type I collagen, and can significantly prevent and / or improve skin aging. BRIEF DESCRIPTION OF DRAWINGS

[0036] Figure 1 is a comparison chart of the type I collagen content detection results of each experimental group in effect example 1.

[0037] Figure 2 This is a comparison chart of the type I collagen content detection results in each experimental group in Example 2. Detailed Implementation

[0038] To make the technical means, inventive features, objectives, and effects of the invention readily understandable, the invention is further illustrated below with reference to specific figures. However, the invention is not limited to the embodiments described below.

[0039] The following effect examples use the Bliss independent model and the Bürgi formula to verify the synergistic effect between the compositions of the present invention.

[0040] The Bliss independent model method uses the incidence of drug-induced adverse events as the efficacy index, with the efficacy value ranging from 0 to 1. Taking drugs A and B as an example, the drug effects of drugs A and B are considered as independent events. Using the probability formula PAB = PA + PB - PA * PB, this formula is used to calculate the expected additive effect of combined drug use: EAB = EA + EB - EA * EB. The ratio of the actual efficacy value to the value calculated by this formula is called CI, and the calculation formula is as follows: CI= CI>1 indicates synergy, CI=1 indicates addition, and CI<1 indicates antagonism.

[0041] The Bliss independent model method introduces the probability summation formula to obtain the efficacy values ​​(incidence rates) of drug A alone, drug B alone, and combination drug A+B from the experiment, and directly calculates the CI value according to the above formula.

[0042] The Bürgi formula, also known as the effect ratio method, is based on the principle that the sum of half the equivalent doses of two drugs should equal the original effect. A sum greater than the original effect indicates a synergistic effect, while a sum less than the original effect indicates an antagonistic effect. The formula for testing this effect is: If q>1, it is synergistic; if q=1, it is additive; if q<1, it is antagonistic. In the formula, a and b are two equivalent doses of drugs A and B, respectively, and E(a) = E(b).

[0043] The red rice bran plant powder in the following examples was sourced from Yunnan Yingge Biotechnology Co., Ltd.

[0044] In the following examples, the % in the 70% alcohol-water solution refers to the percentage of the mass of the corresponding alcohol to the total mass of the alcohol-water solution.

[0045] Example 1 Take a clean beaker and weigh 10g of the above-mentioned red rice bran plant powder. Grind the red rice bran plant powder to a D50 particle size of 20-100μm. Place the ground red rice bran plant powder in the beaker, add 50g of a 70% aqueous solution of butanediol, seal the beaker, and extract by stirring at 35-55℃ for 8-13 hours at a stirring speed of 150-200rpm. Centrifuge to separate the solid and liquid. Filter the supernatant through a 0.50μm filter membrane to obtain the plant extract, then concentrate under reduced pressure to one-third of its volume, filter through a 0.5μm filter membrane, and prepare the test solution with water (hereinafter referred to as the red rice extract solution, with a total solvent volume of 50g). In this example, the alcohol in the 50g 70% aqueous solution of butanediol can be replaced with alkyl monohydric alcohols and / or other alkyl dihydric alcohol solvents.

[0046] According to Method 2 of GB / T 8313-2008, the total polyphenol content of the obtained plant extract was 1.83 mg / mL.

[0047] The solid content of the plant extract was found to be 0.7% (w / w), meaning that 1 mL of the plant extract contained 7 mg of red rice extract, and the density of the plant extract was approximately 1 g / mL.

[0048] Example 1 I. Purpose of Testing and Reference Basis 1.1 Purpose of the test: This test report is an evaluation report on the determination of type I collagen content.

[0049] After determining a suitable sample concentration, HFF-1 cells were inoculated onto plates for 24 hours, and the original cell culture medium was replaced with PBS. The cells were then irradiated with UVA (5 J / cm²). 2 After adding the test sample or control prepared with fresh complete culture medium, incubate for another 24 hours, collect the cell culture medium, and detect the extracellular secretion of type I collagen (Collagen I).

[0050] 1.2 Reference Basis: T / SHRH 031-2020 Cosmetic Firming and Anti-wrinkle Efficacy Test - In Vitro Fibroblast Type I Collagen Content Determination.

[0051] II. Sample Information Sample names: Red rice extract solution and PDRN solution from Example 1 (PDRN molecular weight is 50-60 kDa, component is sodium DNA) Sample quantity and specifications: 1 bottle / 30mL, 1 bottle / 10mL Sample characteristics: The red rice extract solution is a reddish-brown liquid (solvent is butanediol and water = 7:3), and the PDRN solution is a light yellow liquid (solvent is 1,2-pentanediol and water, PDRN content is 0.3-0.5% w / w).

[0052] III. Testing Information 3.1 Detection Principle Type I collagen is a major component of the extracellular matrix of dermal cells. It is synthesized intracellularly by dermal fibroblasts, secreted extracellularly, and then polymerized to form collagen fibers under the action of terminal procollagen peptidase, after the telopeptides separate. Human dermal fibroblasts can be used as a cell model to study the enhancement of type I collagen content in cosmetics. By measuring the upregulation rate of type I collagen content after administration of the test substance, compared with the blank control and the test substance, the efficacy of the test substance in promoting collagen synthesis can be evaluated. Type I collagen content is determined using an enzyme-linked immunosorbent assay (ELISA).

[0053] The specific principle is as follows: Type I collagen specifically binds to collagen antibodies coated on an ELISA plate, and then binds to anti-type I collagen antibodies labeled with a substrate. The substrate is catalyzed by the enzyme to generate a colored product. The content of type I collagen is positively correlated with the intensity of the color of the product. The optical density (OD value) is measured at a wavelength of 450 nm using an ELISA reader to calculate the type I collagen content.

[0054] 3.2 Test Environment All experiments were conducted in a clean, sterile cell culture room, and all reagents and consumables were sterile.

[0055] 3.3 Main Reagents and Consumables Cell culture medium (WINST, high glucose), high-quality fetal bovine serum (Gibco), phosphate-buffered saline (Basal Media, PBS), trypsin (Basal Media, Trypsin, 0.25%), cell culture flasks (Thermo, T25, T75), cell culture plates (Corning, 12-well plate, 96-well plate), pipettes (Thermo, 5mL, 15mL), centrifuge tubes (Corning, 15mL, 50mL), disposable cell counting chamber (Countstar), TGF-β1 (Novoprotein), Human Pro-collagen 1 α1 ELISA Kit (Yisheng Biotechnology).

[0056] 3.4 Instruments and Equipment Adjustable pipette (Eppendorf), analytical balance (BSA124S), inverted microscope, low-speed centrifuge (Likang), constant temperature incubator (Likang), electric pipette (Eppendorf), cell counter (Countstar), clean bench (Thermo), digital display constant temperature water bath (HH-6), liquid nitrogen tank, ordinary refrigerator, full-band microplate reader (MD).

[0057] 3.5 Cell lines The cell line used in this test was human foreskin fibroblast HFF-1 (provided by Fenghui Biotechnology), with cell passages from 7th to 9th.

[0058] 3.6 Method for detecting type I collagen content 1. Cell seeding: Dilute to 1×10⁻⁶ 5 Inoculate at a seeding density of cells / mL, seed cells into 12-well plates with 1 mL / well of cell dilution, and incubate in a cell culture incubator (37℃, 5%CO2, 95%RH) for 24±2 h.

[0059] 2. Experimental groups: Control group, Model group, positive and sample group. This test was set up with 3 replicate wells.

[0060] 3. Solution preparation: 1) Samples: Prepare the sample red rice extract solution and PDRN solution with 1 mL of culture medium (DMEM medium containing 10% FBS and 1% double antibiotics) to concentrations of 0.125%, 0.25%, 0.5%, and 1%. Specifically, prepare four concentrations of red rice extract solution + PDRN solution: 0.0625% + 0.0625% (v / v), 0.125% + 0.125% (v / v), 0.25% + 0.25% (v / v), and 0.5% + 0.5% (v / v) (v / v refers to the volume of red rice extract solution or PDRN solution / the volume of culture medium). Taking a 1mL solution of 0.0625% red rice extract as an example, the actual concentration of red rice extract was calculated to be 4.375ppm, as detailed below: 1 mL of 0.0625% red rice extract solution contains 0.000625 mL of red rice extract solution. According to Example 1, 1 mL of red rice extract solution contains 7 mg of red rice extract. Therefore, 0.000625 mL of red rice extract solution contains 0.004375 mg of red rice extract. That is, the actual concentration of red rice extract in the 0.0625% red rice extract solution is 0.004375 mg / L = 4.375 ppm.

[0061] 2) Positive control: TGF-β1 stock solution diluted to 100 ng / mL; 3) Type I collagen standards: At 4°C, remove 100 ng of each lyophilized standard powder and centrifuge at 10,000 rpm for 30 seconds. Dissolve each standard in 1 mL of sample diluent, and gently pipette 5-8 times to aid dissolution. Prepare standard S7 and set aside. Arrange seven 1.5 mL centrifuge tubes (S0-S6) sequentially, and add 250 μL of sample diluent to each. Transfer 250 μL of standard S7 to the first centrifuge tube (S6) and gently pipette to mix. Transfer 250 μL from S6 to the second EP tube (S5) and gently pipette to mix. Repeat this serial dilution process for the standards. S0 is the sample diluent.

[0062] 4. Induction of sample loading: When the cell confluence in the 12-well plate reaches 80% or higher, add 5 J / cm². 2 After UVA induction, wash twice with PBS, and replace each group with 1 mL of PBS. The blank control group is not subjected to UVA induction. After adding the samples in step 1) according to the requirements of each group, place the 12-well plate in an incubator (37℃, 5%CO2, 95%RH) and incubate for 24±2 h.

[0063] 5. Detection: Detect the standards using ELISA and plot a standard curve. Then, detect the COL I content in the supernatant of each group from "4. Induction of Sample Addition" (e.g., ...). Figure 1 (As shown in the middle and vertical axes), the detection results are shown in Table 1 and Figure 1 As shown, Figure 1 The light filter in the text refers to a red rice extract solution. The experiments in each group are as follows: Control group: Added with an equal amount of cell culture medium; Negative control group (Model): 5 J / cm 2 After UVA irradiation induction, an equal volume of cell culture medium was added; Positive control group: 5 J / cm 2 After UVA irradiation induction, an equal volume of cell culture medium containing 100 ng / mL TGF-β1 was added; Sample group: 5J / cm 2 After UVA irradiation induction, cell culture medium containing the corresponding concentration of the sample was added; Compared to the blank control group, ###, P <0.001; Compared to the negative control group, **, P <0.01, ***, P <0.001; Mean±SD, n=3.

[0064] Table 1. Test data and synergistic effect of type I collagen from red rice extract solution and PDRN solution. Results: Based on cell experiments, the Col I content in HFF-1 cells after UVA induction was measured as follows: (1) Compared with the blank control group, the Col I content in the negative control group was significantly reduced, proving that the model was successfully constructed; (2) Compared with the negative control group, the positive control group with 100 ng / mL TGF-β1 significantly increased the Col I content, further proving that the model was successfully constructed; (3) Compared with the negative control group, red rice extract solutions and PDRN solutions at concentrations of 0.125%, 0.25%, 0.5%, and 1% significantly increased Col I content, with increases of 17.83%, 18.87%, 46.07%, and 87.08%, 19.47%, 37.15%, 53.05%, and 96.44%, respectively. The combination of red rice extract solutions and PDRN solutions at concentrations of 0.0625%+0.0625%, 0.125%+0.125%, 0.25%+0.25%, and 0.5%+0.5% significantly increased Col I content, with increases of 56.17%, 74.89%, 101.79%, and 136.26%, respectively. (4) According to the Bliss independent model method, the CI values ​​of the red rice extract solution + PDRN solution with four concentrations of 0.0625%+0.0625%, 0.125%+0.125%, 0.25%+0.25%, and 0.5%+0.5% were 1.660, 1.528, 1.363, and 1.369, respectively, all of which were greater than 1, indicating a synergistic effect; (5) According to the Bürgi formula, the q-values ​​of the 0.0625% red rice extract solution + 0.0625% PDRN solution combination with 0.125% red rice extract solution are 3.150 and 2.885 respectively; the q-values ​​of the 0.125% red rice extract solution + 0.125% PDRN solution combination with 0.25% red rice extract solution are 3.969 and 2.016 respectively; the q-values ​​of the 0.25% red rice extract solution + 0.25% PDRN solution combination with 0.5% red rice extract solution are 2.209 and 1.919 respectively; the q-values ​​of the 0.5% red rice extract solution + 0.5% PDRN solution combination with 1% red rice extract solution are 1.565 and 1% respectively. The q value of the PDRN solution was 1.413; the q values ​​of the four groups of compound red rice extract solutions + PDRN solutions were all greater than 1, indicating a synergistic effect.

[0065] Example 2 The samples in Table 2 were tested according to the method in Example 1, and the test results are as follows: Figure 2 As shown in Table 2. The green tea extract was from Shanghai Huilang Chemical Co., Ltd., and its origin is England.

[0066] Table 2. Test data and synergistic effects of red rice extract, green tea extract, and PDRN solution on type I collagen. Note: The concentration of solid substances in green tea extract and the concentration of active solid substances in red rice extract are the same in the green tea extract solution. The samples numbered 1, 2, 4, 6, 7, and 9 in Table 2 are the same as those tested in Example 1. The differences in the test results are due to experimental errors and do not affect the comparison results between the experimental groups.

[0067] As shown in Table 2, if the red rice extract is replaced with green tea extract, whose main component is also polyphenols, the CI value obtained according to the Bliss independent model is much less than 1; at the same time, the q value of the compound group and PDRN obtained according to the Bürgi formula is also less than 1. These all indicate that there is no synergistic effect between green tea extract and PDRN.

[0068] Based on the above in vitro cell experiments, it can be seen that the combination of red rice extract solution and PDRN solution at concentrations of 0.125%, 0.25%, 0.5% and 1% can significantly upregulate the secretion of type I collagen, and the combination of red rice extract solution and PDRN solution has a synergistic effect.

[0069] The preferred embodiments of the present invention have been described in detail above. It should be understood that those skilled in the art can make numerous modifications and variations based on the concept of the present invention without creative effort. Therefore, all technical solutions that can be obtained by those skilled in the art based on the concept of the present invention through logical analysis, reasoning, or limited experimentation on the basis of existing technology should be within the scope of protection defined by the claims.

Claims

1. A composition for preventing and / or improving skin aging, characterized by comprising, It comprises red rice extract and PDRN.

2. The composition for preventing and / or improving skin aging according to claim 1, wherein The red rice extract comprises phenolic compounds; The phenolic compounds comprise polyphenolic compounds and phenolic acid compounds; The polyphenolic compounds comprise anthocyanins and / or proanthocyanidins; the polyphenolic compounds account for more than 6% of the total mass of the red rice extract, for example 10%, 20%, 25%, 26.1%, 30%, 40% or 50%.

3. The composition for preventing and / or improving skin aging according to claim 1, wherein The preparation method of the red rice extract comprises the following steps: after red rice is extracted with an aqueous alcohol solution, the supernatant is collected by solid-liquid separation; and the substance obtained after removing the solvent from the supernatant is the red rice extract.

4. The composition for preventing and / or improving skin aging according to claim 3, wherein The alcohol is an alkyl dihydric alcohol; And / or, the mass ratio of the red rice to the aqueous alcohol solution is 1: (4-19); And / or, the mass concentration of the alcohol in the aqueous alcohol solution is 30-80%; And / or, the solid-liquid separation is centrifugation; And / or, in the supernatant, the mass content of the red rice extract is more than 0.5%.

5. The composition for preventing and / or improving skin aging according to claim 4, wherein The alkyl dihydric alcohol is 1,2-propanediol; And / or, the mass ratio of the red rice to the aqueous alcohol solution is 1:5; And / or, the mass concentration of the alcohol in the aqueous alcohol solution is 40%, 50%, 60% or 70%; And / or, in the supernatant, the mass content of the red rice extract is 0.5-1%, for example 0.7%.

6. The composition for preventing and / or improving skin aging according to any one of claims 1 to 5, wherein In the composition, the mass ratio of the red rice extract to the PDRN is 1: (0.1-1), for example 1:0.400, 1:0.420, 1:0.500, 1:0.600, 1:0.700, 1:0.714, 1:0.800 or 1:0.900; And / or, the composition further comprises a solvent, which comprises one or more of water, an alkyl dihydric alcohol and an alkyl monohydric alcohol; the alkyl monohydric alcohol is preferably ethanol; and the alkyl dihydric alcohol preferably comprises one or more of 1,2-propanediol, butanediol and 1,2-pentanediol.

7. The composition for preventing and / or improving skin aging according to claim 6, wherein In the composition, the concentration of the red rice extract is more than 4 ppm; And / or, in the composition, the concentration of the PDRN is more than 1 ppm.

8. The composition for preventing and / or improving skin aging according to claim 7, wherein In the composition, the concentration of the red rice extract is 4-50 ppm, for example 4.375 ppm, 8.75 ppm, 17.5 ppm, 35 ppm or 50 ppm; And / or, in the composition, the concentration of the PDRN is 1-30 ppm, for example 1.875 ppm, 3 ppm, 3.75 ppm, 6.25 ppm, 7.5 ppm, 12.5 ppm, 15 ppm, 25 ppm or 30 ppm.

9. A composition for promoting production of collagen type I, characterized by, It comprises the red rice extract according to any one of claims 1-8 and the PDRN according to any one of claims 1-8.

10. A cosmetic product, characterized by, It comprises the composition for preventing and / or improving skin aging according to any one of claims 1-8 or the composition for promoting the production of type I collagen according to claim 9; and the cosmetic product comprises a makeup cosmetic product, a base cosmetic product, a skin care product or a sunscreen cosmetic product.

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