Hydrogen sulfide oxidizing bacterium and application thereof in desulfurization process

Abstract

The invention belongs to the technical field of environmental microorganism screening application, and particularly relates to a hydrogen sulfide oxidizing bacterium and application thereof in a desulfurization process. The invention provides a hydrogen sulfide oxidizing bacterium (Acinetobacter sp. S-10, the preservation unit is China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.35086) which can be cultured in seawater and fresh water and has the characteristic of low temperature resistance, and a hydrogen sulfide oxidizing bacterium strain (Acinetobacter sp. S-10, the preservation unit is China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.35086). The hydrogen sulfide oxidizing bacteria can oxidize hydrogen sulfide in low-temperature, high-salt and strong-alkali environments to produce thiosulfate, a brand new path is opened up for innovation of a biological desulfurization process technology in an extreme environment, conversion from pollutants to high-value resources is realized by directional regulation and control of sulfur metabolites, and meanwhile, the hydrogen sulfide oxidizing bacteria have a wide application prospect. The hydrogen sulfide oxidizing bacteria can grow in seawater and fresh water, and the economical efficiency of the whole treatment process is remarkably improved.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of environmental microbial screening application, and particularly relates to a hydrogen sulfide oxidizing bacterium and application thereof in a desulfurization process. BACKGROUND

[0002] The information disclosed in the background of the present application is only intended to increase the understanding of the overall background of the present application, and is not necessarily regarded as acknowledging or implying in any form that the information constitutes prior art known to those skilled in the art.

[0003] Hydrogen sulfide is a colorless gas with a strong rotten egg smell. Through a series of chemical reactions such as photo-oxidation and free radical reaction, hydrogen sulfide can generate sulfur dioxide, which further forms sulfate aerosol, and drives the formation of acid rain (pH<5.6) through wet or dry deposition, resulting in air / water pollution, material corrosion and biological toxicity. Therefore, it is essential to develop an efficient desulfurization process, which has high application value.

[0004] Sulfur-oxidizing microorganisms play an important role in the process of sulfur oxidation as the driver of biological oxidation. They have the characteristics of low cost, small pollution, environmental friendliness and sustainability, and can oxidize elemental sulfur or various inorganic sulfur compounds in reduced states to sulfuric acid or higher valence sulfides. This technology mainly utilizes the synergistic effect of sulfate-reducing bacteria (SRB) and sulfur-oxidizing bacteria (SOB) to achieve the treatment of sulfate in wastewater, realizing the transformation from pollutants to resources. However, in the actual wastewater treatment process, extreme environmental conditions (such as high salt, low temperature or excessively high pH value) may affect the enzyme activity of SRB and SOB, resulting in impaired metabolic function, thus restricting the application effect of biological desulfurization technology in complex wastewater treatment.

[0005] The deep-sea environment has extreme characteristics such as high pressure, low temperature and high salt, which prompts the microorganisms inhabiting therein to evolve unique adaptive mechanisms. Macro-genomic analysis shows that the diversity of sulfur-oxidizing related genes in deep-sea microbial communities far exceeds that of terrestrial strains, which provides rich genetic resources for exploring efficient hydrogen sulfide oxidizing bacteria. Therefore, it is of great significance to optimize the biological desulfurization process and expand its application under extreme conditions by screening strains with strong environmental adaptability and high sulfur-oxidizing capacity from deep-sea microorganisms. SUMMARY

[0006] Therefore, the present application provides a hydrogen sulfide oxidizing bacterium and application thereof in a desulfurization process. The bacterium can grow in seawater and freshwater, and has higher salt tolerance, lower temperature tolerance and stronger alkaline tolerance, and can remove hydrogen sulfide in the environment under extreme environments.

[0007] To achieve the above objectives, the present invention is implemented through the following technical solution: In a first aspect, the present invention provides a sulfur-oxidizing bacterium, wherein the sulfur-oxidizing bacterium is Acinetobacter, and the bacterium is named Acinetobacter. Acinetobacter sp S-10, the Acinetobacter bacillus Acinetobacter sp S-10 was deposited on July 2, 2025, at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, 100101, China, with accession number CGMCC No. 35086.

[0008] The Acinetobacter bacillus of this invention was isolated from deep-sea sediment samples in the western Pacific Ocean obtained by the Jiaolong submersible during the 80th Chinese oceanographic expedition. It exhibits the characteristic of oxidizing hydrogen sulfide to produce thiosulfate under low-temperature, high-alkaline conditions. This bacterium is a chemoheterotrophic microorganism, deriving its cellular energy and carbon source from organic matter in the culture medium. It can grow under high-alkaline, low-temperature conditions at pH 7.8–9.0 and 10–15°C.

[0009] Preferably, the strain is used in one or more of the following forms: live bacteria, inactivated bacterial cell fermentation broth, exosomes, or their metabolites.

[0010] The present invention also provides a method for culturing the Acinetobacter, the method comprising culturing the bacteria at low temperature and at room temperature on 1 / 2 YTSS sea salt medium and LB fresh water medium.

[0011] Preferably, the 1 / 2 YTSS culture medium comprises: 2.5 g / L tryptone, 4 g / L yeast extract, and 20 g / L sea salt; Preferably, the LB medium comprises: 10 g / L tryptone, 5 g / L yeast extract, and 10 g / L sodium chloride.

[0012] Preferably, the pH value of the culture medium is 7.8~9.0; the culture temperature is 10~15℃ at low temperature and 30~37℃ at room temperature.

[0013] Preferably, the strain produces thioquinone oxidoreductase (SQR) to oxidize sulfide to thioalkyl sulfide, and produces persulfide dioxygenase (PDO) to further oxidize thioalkyl sulfide to thiosulfate.

[0014] Secondly, the present invention provides a microbial inoculant comprising the aforementioned strain Acinetobacter. Acinetobacter sp S-10.

[0015] Preferably, the use of the microbial agent in the preparation of a hydrogen sulfide removal product.

[0016] In a third aspect, the present application provides the hydrogen sulfide oxidizing bacteria Acinetobacter baylyi S-10 of the first aspect of the present application or the use of the microbial agent of the second aspect in the removal of hydrogen sulfide. Acinetobacter sp . S-10 or the microbial agent oxidizes hydrogen sulfide into thiosulfate.

[0017] Preferably, the hydrogen sulfide oxidizing bacteria Acinetobacter baylyi S-10 or the microbial agent oxidizes hydrogen sulfide into thiosulfate. Acinetobacter sp . S-10 or the microbial agent oxidizes hydrogen sulfide into thiosulfate.

[0018] In a fourth aspect, the present application provides a method for biologically removing hydrogen sulfide, which specifically comprises the following steps: (1) culturing the hydrogen sulfide oxidizing bacteria Acinetobacter baylyi S-10; Acinetobacter sp (2) contacting a water body or gas containing hydrogen sulfide with the cultured hydrogen sulfide oxidizing bacteria Acinetobacter baylyi S-10; and Acinetobacter sp using the hydrogen sulfide oxidizing bacteria to treat sulfur-containing gas and solution to oxidize hydrogen sulfide into thiosulfate.

[0019] Preferably, in the step (1), the hydrogen sulfide oxidizing bacteria is prepared into a microbial agent or is immobilized in a biological sponge filler.

[0020] Preferably, the immobilization method is as follows: under sterile conditions, 10 mL of the target strain is inoculated into 1 L of fresh 1 / 2 YTSS culture medium, and incubated at 30°C for 6 h; when the bacteria enter the early logarithmic growth phase, elemental sulfur S8 is added for induction; and after induction, biological sponge filler is added to the bacterial culture solution to allow the bacteria to be adsorbed and immobilized.

[0021] Preferably, in the step (2), the salinity is 1.9%-2.0%, the pH is 7.8-9.0, and the temperature is normal temperature 25-30°C; the salinity of the conventional bacterial culture medium is 0.5%-1.0%.

[0022] In a fifth aspect, the present application provides a device for biologically removing hydrogen sulfide, which comprises: The constructed biological desulfurization device comprises an anaerobic biological reactor (1), an aerobic biological desulfurization tower (6), and an alkali liquid pool (13); The bottom of the alkali liquid pool is provided with an alkali liquid outlet valve (14) connected to a spraying port (11) at the top of the aerobic biological desulfurization tower, and the side of the aerobic biological desulfurization tower is provided with a hydrogen sulfide gas inlet valve (8) connected to a hydrogen sulfide gas outlet valve (3) at the top of the anaerobic biological reactor; The aerobic biological desulfurization tower comprises biological sponge filler for immobilizing the deep-sea hydrogen sulfide oxidizing bacteria Acinetobacter baylyi S-10. Acinetobacter sp . S-10.​

[0023] Preferably, the lye tank top is also provided with lye inlet valve (15); the bottom of the lye tank lye outlet valve is connected to the top of the aerobic biological desulfurization tower by flow pump (12); the top of the aerobic biological desulfurization tower is also provided with a treated gas detection valve (10), the bottom is provided with a liquid valve (9) and an exhaust valve (7); the anaerobic biological reactor is also provided with an argon inlet valve (2), an untreated gas detection valve (4) and a pressure gauge (5).

[0024] Preferably, in the biological desulfurization process device, hydrogen sulfide gas or solution containing hydrogen sulfide is introduced into the constructed aerobic biological desulfurization tower by argon or spraying; the hydrogen sulfide oxidizing bacteria are used to treat hydrogen sulfide gas and solution, and hydrogen sulfide is oxidized to thiosulfate.

[0025] Preferably, the immobilization method is as follows: under sterile conditions, 10 mL of target strain is inoculated in 1 L of fresh 1 / 2YTSS medium, incubated at 30 DEG C for 6-8 h, and S8 is added when the bacteria enter the early logarithmic growth phase, and then biological sponge filler is added in the bacterial culture solution to allow the bacteria to be adsorbed and fixed.

[0026] Preferably, the aerobic biological desulfurization tower can detect the residual amount of hydrogen sulfide in gas and liquid from the top and bottom.

[0027] Preferably, the lye tank sprays lye into the aerobic biological desulfurization tower, increases the solubility of hydrogen sulfide gas, reduces volatilization, and the pH of the lye is 7.8-9.0.

[0028] The constructed aerobic biological desulfurization tower can realize a sulfide removal rate of 90% under low temperature, high salt and strong alkali extreme environment, and generate thiosulfate with industrial economic value.

[0029] Compared with the prior art, the present application has the following beneficial effects: The alkali-resistant and low-temperature Acinetobacter provided by the present application Acinetobacter sp. S-10 can oxidize hydrogen sulfide to thiosulfate under high-alkali, high-salt and low-temperature conditions, significantly improving the efficiency and capacity of removing hydrogen sulfide in extreme environments, greatly reducing environmental pollution, and the hydrogen sulfide oxidizing bacteria can grow in seawater and freshwater, reducing treatment cost and improving economic value. BRIEF DESCRIPTION OF DRAWINGS

[0030] The drawings accompanying the specification of the present application serve to provide a further understanding of the present application, and the illustrative embodiments of the present application and their descriptions serve to explain the present application, and do not constitute an improper limitation on the present application.

[0031] Figure 1 : The Acinetobacter of the present applicationAcinetobacter sp. Phylogenetic tree of strain S-10; Figure 2 Acinetobacter of the present invention Acinetobacter sp. Microscopic images of strain S-10; Figure 3 Acinetobacter of the present invention Acinetobacter sp. Growth curves of strain S-10 at 15℃ and 30℃, respectively; Figure 4 Acinetobacter of the present invention Acinetobacter sp. The sulfurization and oxidation rate and product formation of strain S-10 in 1 / 2 YTSS medium at 15℃; Figure 5 Design drawing (A) and screen plate design drawing (B) of a multi-stage biological desulfurization process unit; Figure 6 Image showing the desulfurization effect of an aerobic biological desulfurization tower; Figure 5 Component A Description: 1. Anaerobic bioreactor; 2. Argon inlet valve; 3. Hydrogen sulfide gas outlet valve; 4. Untreated gas detection valve; 5. Pressure gauge; 6. Aerobic biological desulfurization tower; 7. Exhaust valve; 8. Hydrogen sulfide gas inlet valve; 9. Liquid extraction valve; 10. Treated gas detection valve; 11. Spray nozzle; 12. Flow pump; 13. Alkali tank; 14. Alkali outlet valve; 15. Alkali inlet valve. Detailed Implementation

[0032] It should be noted that the following detailed descriptions are exemplary and intended to provide further illustration of the invention. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains.

[0033] The technical solution of the present invention will be further described below with reference to specific embodiments.

[0034] Example 1: Acinetobacter sulfide hydroxide Acinetobacter sp. Screening and separation of S-10 and its performance in producing thiosulfate through low-temperature sulfur oxidation. Deep-sea sediments from the western Pacific Ocean were collected. One g of sample was placed in 1 / 2 YTSS sea salt medium, with the following composition: 2.5 g / L tryptone, 4 g / L yeast extract, 20 g / L sea salt, and pH adjusted to 8.0 with 1 M NaOH. The culture was incubated at 15℃ and 200 rpm for 3 days. 10 mL of the suspension was then inoculated into 100 mL of the same 1 / 2 YTSS medium and incubated for another 3 days at 15℃ and 200 rpm. The enriched culture was then isolated using the streak plate method. Single colonies were picked and inoculated into test tubes containing 1 / 2 YTSS sea salt medium. Lead acetate test paper was placed at the mouth of the test tube for further screening. Six strains that could turn lead acetate test paper black were initially obtained.

[0035] The 6 strains of bacteria were respectively inoculated into 1 / 2YTSS sea salt medium in a test tube for activation culture. The activated bacteria were transferred into fresh 100 mL 1 / 2YTSS sea salt medium, and the initial OD 600 = 0.02 was adjusted, and hydrogen sulfide was added to induce for 2-3 h at the logarithmic growth phase. The bacteria were collected by centrifugation at 4000 rpm for 10 min. The supernatant was removed, and the bacteria were washed twice with 50 mM pH 8.4 Tris-HCl buffer, and then resuspended to OD 600 = 3. 10 mL of the cell suspension was taken into a 50 mL centrifuge tube, 500 μM of hydrogen sulfide was added and quickly sealed, and then placed in a 15℃, 200 rpm shaking incubator. The substrate and product contents in the reaction were measured every 0.5 h from 0 h to 3 h.

[0036] After screening and repeated separation and purification of the hydrogen sulfide metabolism ability, a hydrogen sulfide oxidizing bacterium S-10 was obtained, which had a semi-transparent white color, was moist, had a medium size, and had a round edge. Gram staining was negative, and the bacterial morphology was bacillus, as shown in the microscope photograph. Figure 2

[0037] 2 μL of the tested bacterial liquid was taken as the template DNA for amplification and sequencing of the 16S rRNA of the strain. The 16S rDNA sequence of the purified strain was amplified by PCR using bacterial universal primers, and the primers were 27f (5'-AGAGTTTGAT CMTGCCTCAG-3') and 1492r (5'-TACGGYTACCTTGTTACGACTT-3'). The 16S rDNA sequence was submitted to NCBI (https: / / www.ncbi.nlm.nih.gov / ) for BLAST comparison and analysis. The results showed that the homology with Acinetobacter sp. RAG-1 was 99%, and the hydrogen sulfide oxidizing bacterium was identified as an Acinetobacter. The constructed phylogenetic tree is shown in Figure 1

[0038] The prepared resting cells were detected for hydrogen sulfide metabolism rate and product. The hydrogen sulfide oxidizing bacterium could grow at 15℃ low temperature and 30℃ normal temperature, and oxidize hydrogen sulfide to produce thiosulfate, as shown in Figure 3 and Figure 4

[0039] This example illustrates that the isolated hydrogen sulfide oxidizing bacterium Acinetobacter Acinetobacter sp. S-10 has the ability to oxidize hydrogen sulfide to produce thiosulfate. ​​​

[0040] Example 2: Acinetobacter Acinetobacter sp. S-10 for removing hydrogen sulfide in water body for aquaculture Acinetobacter Acinetobacter sp. S-10 for removing hydrogen sulfide produced in water body for aquaculture is as follows: first, the low temperature preservation of Acinetobacter Acinetobacter sp. S-10 is activated in a test tube containing 5-10 mL of 1 / 2YTSS sea salt medium (2.5 g / L tryptone; 4 g / L yeast extract; 20 g / L sea salt; 1 L distilled water). The activated strain is transferred into 100-200 mL of culture medium, and after 15-20 h of culture, it is expanded to 300-500 mL as seed liquid at a 2-5% inoculation amount.

[0041] The above seed liquid is inoculated into a primary fermenter at a 1% inoculation amount for expansion culture, and samples are taken for microscopic examination at regular intervals. When the strain grows for 6 h and the OD 600 is 1.69, it is transferred into a secondary fermenter at a 5-10% inoculation amount for further expansion culture for 20 h to obtain a fermentation liquid.

[0042] The bacterial liquid is passed into a GQ150 tubular centrifuge, and after high-speed centrifugation by a rotating drum, the liquid is removed to obtain a block-shaped bacterial sludge. The block-shaped bacterial sludge is further mixed with a protective agent and water in a certain proportion, the bacterial sludge is fully dispersed and emulsified to form a liquid. The liquid is poured into freeze-drying trays in a vacuum freeze-drying machine, and vacuum freeze-drying is performed at -40℃ for 70 h to form a freeze-dried bacterial powder.

[0043] The above freeze-dried bacterial powder is mixed with malt dextrin twice the weight of the freeze-dried powder and 10% white carbon black, and packaged into the required bacterial agent.

[0044] The Acinetobacter Acinetobacter sp S-10 bacterial agent is dissolved in water, fully dissolved and mixed, and then uniformly added to the target water body, so that about 100 billion single bacteria per gram of water body are contained in the aquaculture site. The experimental time is in autumn, and the water temperature is 10-15℃. Water samples are taken every 1 d to detect the content of hydrogen sulfide, and after 7-9 d of reaction, the removal rate of hydrogen sulfide is 90%.

[0045] This example illustrates that Acinetobacter Acinetobacter sp S-10 for removing hydrogen sulfide in water body for aquaculture can be carried out in the case of lower air temperature in autumn and winter, and can be cultured in seawater and freshwater, significantly improving the efficiency and ability to remove hydrogen sulfide produced in aquaculture in extreme environments, greatly reducing the persecution of hydrogen sulfide on aquatic organisms, and improving the value of aquaculture.

[0046] Example 3: Acinetobacter Acinetobacter sp. S-10 for treating hydrogen sulfide-containing gas in an aerobic biological desulfurization tower Acinetobacter baylyi Acinetobacter sp. The step for treating the hydrogen sulfide-containing gas by the aerobic biological desulfurization tower of S-10 is as follows: the immobilized Acinetobacter baylyi S-10 is placed in the aerobic biological desulfurization tower in the biological desulfurization process device. Acinetobacter sp. The control group biological sponge filler is placed in the sterile culture medium and shaken and soaked.

[0047] In the anaerobic biological reactor of the biological desulfurization process device, the sulfate solution and sulfate-reducing bacteria are added to generate hydrogen sulfide gas by sulfate reduction.

[0048] The alkali solution with a pH of 7.8-9.0 is placed in an alkali pool, and the anaerobic biological reactor, the aerobic biological desulfurization tower and the alkali pool are connected as shown in the figure. Figure 5

[0049] The argon is introduced, the connection valves of the three parts are opened, the hydrogen sulfide gas is discharged into the aerobic biological desulfurization tower, the flow pump is opened to spray the alkali solution into the aerobic biological desulfurization tower, and the Acinetobacter baylyi S-10 oxidizes the hydrogen sulfide in the gas and the liquid into thiosulfate. Acinetobacter sp.

[0050] The preparation method of the immobilized Acinetobacter baylyi S-10 is as follows: the Acinetobacter baylyi is cultured in a 2 L triangular flask by using 1 / 2YTSS sea salt culture medium. Acinetobacter sp. Acinetobacter sp. When the bacteria enter the early logarithmic growth phase, 50 μM S8 is added for induction for 2 h, 60 pieces of biological sponge fillers are added into the bacterial culture solution after induction, and incubation is performed for 48 h to enable the bacteria to be adsorbed and fixed.

[0051] After 5 min of reaction, the liquid valve and the exhaust valve are opened, and the residual amount of hydrogen sulfide in the liquid and the gas is detected as shown in the figure. Figure 6

[0052] The experimental results show that the biological desulfurization process device constructed in the application can effectively remove the hydrogen sulfide in the environmental liquid and gas, the removal rate reaches 89%, and thiosulfate with industrial application value is generated.

[0053] The example shows that the Acinetobacter baylyi S-10 is used for treating the hydrogen sulfide-containing gas by the aerobic biological desulfurization tower, the high-alkali alkali solution can be used for spraying, the volatilization of hydrogen sulfide is reduced, the efficiency and the ability of removing the hydrogen sulfide in the extreme environment are significantly improved, the environmental pollution is greatly reduced, the treatment cost is reduced, and the economic value is improved. Acinetobacter sp.

[0054] ​​​​​The above merely provides the preferred embodiments of the present application, and is not used to limit the present application. For those skilled in the art, the present application can have various modifications and changes. Any modifications, equivalent replacements, improvements, etc. made within the principles and technical scope of the present application shall fall into the scope of the present application.

Claims

1. A hydrogen sulfide oxidizing bacterium, characterized in that, The hydrogen sulfide oxidizing bacteria is Acinetobacter, and the name of the bacteria is Acinetobacter Acinetobacter sp . S-10, and the preservation number is CGMCC No. 35086.

2. The hydrogen sulfide oxidizing bacteria of claim 1, wherein, The use form of the strain includes one or more of live bacteria, inactivated bacteria fermentation broth, exosome, or metabolite thereof.

3. A microbial inoculant, characterized in that, including said acinetobacter Acinetobacter sp . S-10.

4. Use of the hydrogen sulfide-oxidizing bacteria according to claim 1 or 2 or the microbial inoculum according to claim 3 for the removal of hydrogen sulfide; preferably the hydrogen sulfide-oxidizing bacteria are Acinetobacter sp. Acinetobacter sp . S-10 or the microbial inoculum oxidizes hydrogen sulfide to thiosulfate.

5. A method for biologically removing hydrogen sulfide, characterized by, The method comprises the following steps: (1) Cultivation of Acinetobacter sulfide hydroxide Acinetobacter sp S-10; (2) contacting a water body or gas containing hydrogen sulfide with a cultured hydrogen sulfide oxidizing bacterium Acinetobacter johnsonii Acinetobacter sp S-10 Contacting; using the hydrogen sulfide oxidizing bacteria to treat sulfur-containing gases and solutions to oxidize hydrogen sulfide to thiosulfate.

6. The method of claim 5, wherein, In step (1), the hydrogen sulfide oxidizing bacteria are prepared into a microbial inoculant or fixed in a biological sponge filler. Preferably, the immobilization method is as follows: under sterile conditions, 10 mL of the target strain is inoculated into 1 L of fresh 1 / 2YTSS medium, and incubated at 30°C for 6 h; when the bacteria enter the early logarithmic growth phase, S8 induction is added; and after induction, the biological sponge filler is added to the bacterial culture solution to allow the bacteria to be adsorbed and fixed.

7. The method of claim 5, wherein, In step (2), the salinity is 1.9%-2.0%, the pH is 7.8-9.0, and the temperature is 25-30°C.

8. An apparatus for biologically removing hydrogen sulfide, characterized by The method comprises the following steps: The constructed biological desulfurization device comprises an anaerobic biological reactor (1), an aerobic biological desulfurization tower (6), and an alkali liquid pool (13); The bottom of the alkali liquid pool is provided with an alkali liquid outlet valve (14) connected to the top spraying port (11) of the aerobic biological desulfurization tower, and the side of the aerobic biological desulfurization tower is provided with a hydrogen sulfide gas inlet valve (8) connected to the top hydrogen sulfide gas outlet valve (3) of the anaerobic biological reactor; The aerobic biological desulfurization tower includes immobilized deep-sea hydrogen sulfide oxidizing bacteria Acinetobacter Acinetobacter sp . Biological sponge filler of S-10.

9. The apparatus of claim 8, wherein, The aerobic biological desulfurization tower can detect the residual amount of hydrogen sulfide in the gas and liquid from the top and the bottom; Preferably, the alkali liquid pool sprays alkali liquid into the aerobic biological desulfurization tower, and the pH of the alkali liquid is 7.8-9.0; Preferably, the top of the alkali liquid pool is further provided with an alkali liquid inlet valve (15); Preferably, the alkali liquid outlet valve at the bottom of the alkali liquid pool is connected to the top spraying port of the aerobic biological desulfurization tower through a flow pump (12); Preferably, the top of the aerobic biological desulfurization tower is further provided with a treated gas detection valve (10), the bottom is provided with a liquid taking valve (9), and the bottom is provided with an exhaust valve (7); Preferably, the anaerobic biological reactor is further provided with an argon gas inlet valve (2), an untreated gas detection valve (4), and a pressure gauge (5).

10. The apparatus of claim 8, wherein, In the biological desulfurization process device, hydrogen sulfide gas or a solution containing hydrogen sulfide is introduced into the constructed aerobic biological desulfurization tower by argon gas or spraying; the hydrogen sulfide gas and the solution are treated by using the hydrogen sulfide oxidizing bacteria to oxidize the hydrogen sulfide into thiosulfate.

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