Method for preventing and treating piglet diarrhea
Functional feed was prepared by co-fermenting the above-ground stems and leaves of Alpinia galanga with Bacillus licheniformis and Bacillus subtilis, which solved the problem of diarrhea in piglets and resource waste, and achieved safe and green prevention and treatment effects.
Patent Information
- Application Number
- CN202511435667.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-10-09
- Publication Date
- 2026-01-27
AI Technical Summary
In existing technologies, antibiotics and high-dose trace elements are commonly used to treat diarrhea in piglets, leading to drug residues and increased drug resistance. Furthermore, the above-ground stems and leaves of Alpinia galanga are not effectively utilized.
A functional feed additive was prepared by co-fermenting the above-ground stems and leaves of Alpinia galanga with wheat bran using Bacillus licheniformis and Bacillus subtilis, and using microbial fermentation technology to prevent and treat diarrhea in piglets.
It significantly reduces the incidence and severity of diarrhea in piglets, promotes piglet growth, improves feed utilization, and enables high-value utilization of the above-ground stems and leaves of Alpinia officinarum, avoiding drug residues and drug resistance.
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Abstract
Description
Technical Field
[0001] This invention belongs to the field of piglet feeding technology, specifically relating to a method for preventing and treating diarrhea in piglets. Background Technology
[0002] In the entire pig production chain, the piglet stage is a fundamental and crucial link. However, due to the incomplete development of piglets' organs and tissues, as well as physiological defects such as immature immune systems and digestive and absorptive functions, piglets are highly susceptible to diarrhea. Weaned piglet diarrhea is a common and frequently occurring disease in large-scale pig farms, and its occurrence is closely related to the immaturity of the digestive system and immune function of piglets after early weaning. Affected piglets not only experience stunted growth, but in severe cases, they may even die.
[0003] Currently, pig farms commonly use antibiotics and high-dose trace elements to control weaning diarrhea. While these methods can reduce the incidence of diarrhea in the short term, long-term use leads to increasingly prominent side effects such as excessive antibiotic residues in livestock products, intestinal flora imbalance, and increased antibiotic resistance in pathogens. Furthermore, environmental pollution caused by drug abuse, such as antibiotic residues in feces contaminating soil and water, poses a potential threat to human health. Therefore, developing environmentally friendly, efficient, and residue-free antibiotic alternatives has become an important research direction.
[0004] Alpinia officinarum Hance, a traditional Chinese medicine, possesses underground rhizomes rich in flavonoids, diarylheptanes, and volatile oils, exhibiting various pharmacological effects such as antibacterial, anti-inflammatory, antioxidant, and immunomodulatory properties, attracting widespread attention in the medical field. However, during the harvesting and processing of Alpinia officinarum, its above-ground stems and leaves (including leaves and stems) are often discarded as agricultural waste, resulting in resource waste. Currently, some studies have explored the use of traditional Chinese medicine and its byproducts as feed additives, but reports on the application of Alpinia officinarum stems and leaves are scarce. Whether microbial fermentation technology can be used to screen strains for biotransformation of Alpinia officinarum stems and leaves, degrading its nutritional factors, improving the bioavailability of its active substances, and developing the fermentation products into functional feed additives for the prevention and treatment of piglet diarrhea, thereby achieving high-value utilization of agricultural waste, remains to be seen. Summary of the Invention
[0005] One objective of this invention is to provide a safe, green, and residue-free solution for the prevention and treatment of diarrhea in piglets, replacing or reducing the use of antibiotics and high-dose trace elements, thereby fundamentally avoiding drug residues and the generation of drug-resistant strains. Another objective of this invention is to realize the high-value utilization of agricultural waste—the above-ground stems and leaves of Alpinia galanga—by transforming it into feed additives with certain efficacy through a specific bio-fermentation process, turning waste into treasure.
[0006] On one hand, the present invention provides a method for preventing and treating diarrhea in piglets, the method comprising the following steps: feeding piglets a functional feed, wherein the preparation method of the functional feed comprises:
[0007] S1. Prepare seed culture of Bacillus licheniformis, wherein the preservation number of Bacillus licheniformis is CICC 10103;
[0008] S2. Prepare seed culture of Bacillus subtilis, wherein the preservation number of Bacillus subtilis is CGMCC 1.9086;
[0009] S3. The seed culture of Bacillus licheniformis prepared in step S1 is mixed with the seed culture of Bacillus subtilis prepared in step S2 at a volume ratio of 1-2:1-2 to obtain a compound fermentation seed culture.
[0010] S4. Prepare a solid-state fermentation medium, wherein the solid-state fermentation medium comprises galangal aerial stem and leaf powder and wheat bran powder, wherein the mass ratio of galangal aerial stem and leaf powder to wheat bran powder is 4-7:6-3.
[0011] S5. Add water to the solid fermentation medium in step S4, the amount of water being equal to the mass of the solid fermentation medium, and then add 3%-5% of the total mass of the solid fermentation medium to the compound fermentation seed liquid prepared in step S3, and mix well.
[0012] S6. Perform two-stage fermentation: the first stage is fermented at 34-36℃ for 24 hours, and the second stage is fermented at 28℃ for 20-24 hours to obtain the fermentation product;
[0013] S7. The fermentation product obtained in step S6 is dried and granulated to obtain the functional feed.
[0014] Furthermore, in the method, in step S3, the mass ratio of the Bacillus licheniformis seed liquid to the Bacillus subtilis seed liquid is 1:1.
[0015] Furthermore, in the method, in step S4, the mass ratio of the galangal above-ground stem and leaf powder to the wheat bran powder is 5:5.
[0016] Furthermore, in the method, in step S5, the composite fermentation seed liquid obtained in step S3 is added at 5% of the total mass of the solid fermentation culture medium.
[0017] Furthermore, in the method, in step S6, the first stage involves fermentation at 35°C for 24 hours.
[0018] Secondly, the invention also provides functional feeds prepared by the method described in this invention.
[0019] Furthermore, the functional feed comprises fermented galangal stem and leaf powder and wheat bran powder, as well as live Bacillus licheniformis and Bacillus subtilis and / or their metabolites.
[0020] Finally, the use of galangal above-ground stems and leaves in the preparation of feed for the prevention and / or treatment of diarrhea in piglets is also provided, wherein the galangal above-ground stems and leaves are co-fermented with Bacillus licheniformis and Bacillus subtilis.
[0021] Compared with the prior art, the technical solution provided by the present invention has at least the following beneficial effects or advantages:
[0022] (1) The method provided by this invention significantly reduces the incidence and severity of diarrhea in piglets. Compared with the blank control group (diarrhea rate of 17.13%), the diarrhea rate of piglets fed the functional feed prepared by this invention decreased to 2.15%-3.03%, and the diarrhea index also decreased from 4.13 to below 1.0. Its effect is far superior to the diarrhea rate of 10.47%-12.35% in the single-strain fermentation group and 13.13% in the unfermented raw material group, indicating that the co-fermentation of the two specific strains produced a synergistic effect and has a significant preventive and therapeutic effect on diarrhea in piglets.
[0023] (2) Adding the functional feed of this invention to the daily basic feed of piglets significantly promotes piglet growth and improves feed utilization. Test results show that the feed conversion ratio of the experimental group of piglets decreased from 1.94 to 1.41-1.54. This indicates that the product of this invention effectively promotes piglet growth while ensuring intestinal health.
[0024] (3) This invention is the first to use the waste part generated during the harvesting of galangal—the above-ground stems and leaves—as the core raw material, and transforms them into functional feed through microbial fermentation technology. This realizes the high-value utilization of the above-ground stems and leaves of galangal.
[0025] (4) Both Bacillus licheniformis and Bacillus subtilis used in this invention are permitted feed additives and are safe and reliable. The final product is rich in probiotics, and the metabolites can maintain intestinal health through biological regulation, reducing antibiotic residues and drug resistance from the source. Detailed Implementation
[0026] The technical solution of the present invention will be described below with reference to the embodiments. However, the present invention is not limited to the following embodiments.
[0027] To enable those skilled in the art to better understand and implement the technical solutions of the present invention, the present invention will be further described below with reference to specific embodiments, but the embodiments are not intended to limit the present invention.
[0028] Unless otherwise specified, the test methods and detection methods described in the following embodiments are conventional methods; unless otherwise specified, the reagents and materials are commercially available.
[0029] Bacillus licheniformis, accession number CICC 10103, was purchased from the China Industrial Microbial Culture Collection Center and is referred to as Bacillus licheniformis CICC 10103 below.
[0030] Bacillus subtilis, accession number CGMCC 1.9086, was purchased from the China General Microbiological Culture Collection Center and is referred to as Bacillus subtilis CGMCC 1.9086 below.
[0031] Example 1
[0032] This example describes the preparation of seed culture of Bacillus licheniformis CICC 10103.
[0033] Bacillus licheniformis CICC 10103 was activated on NB solid culture plates. Single colonies were picked and inoculated into NB liquid culture medium and incubated at 30℃ and 200 rpm for 16-24 hours to achieve a concentration of 2 × 10⁻⁶ for Bacillus licheniformis CICC 10103. 6 CFU / mL is the seed culture of Bacillus licheniformis CICC 10103.
[0034] Example 2
[0035] This example describes the preparation of seed culture of Bacillus subtilis CGMCC 1.9086.
[0036] Bacillus subtilis CGMCC 1.9086 was activated on NB solid medium. Single colonies were picked and inoculated onto NB liquid medium and incubated at 32°C and 200 rpm for 16-24 hours to achieve a final concentration of 2 × 10⁻⁶ Bacillus subtilis CGMCC 1.9086. 6 CFU / mL is the seed culture of Bacillus subtilis.
[0037] Example 3
[0038] This embodiment describes the preparation of functional feed using the above-ground stems and leaves of Alpinia galanga as substrate and co-fermentation with Bacillus licheniformis CICC 10103 and Bacillus subtilis CGMCC 1.9086.
[0039] Preparation of solid-state fermentation culture medium:
[0040] The aerial stems and leaves of Alpinia galanga were dried to constant weight in a drying oven at 60℃, and then pulverized through a 100-mesh sieve to obtain Alpinia galanga aerial stem and leaf powder. Wheat bran was subjected to high-pressure steam treatment at 160℃ for 6 minutes, and then dried to constant weight in a drying oven at 60℃. Wheat bran was sieved through a 100-mesh sieve to obtain wheat bran powder. The Alpinia galanga aerial stem and leaf powder and wheat bran powder were mixed at a mass ratio of 7:3, and 1% yeast extract, 0.05% MgSO4, 0.01% MnSO4 and 0.005% CuSO4 were added and mixed evenly to obtain a solid-state fermentation medium.
[0041] The seed culture of Bacillus licheniformis CICC 10103 prepared in Example 1 and the seed culture of Bacillus subtilis CGMCC 1.9086 prepared in Example 2 were mixed at a volume ratio of 2:1 to obtain a compound fermentation seed culture.
[0042] Add solid-state fermentation medium and an equal volume of water, mix well, then add 3% (by weight of total solid-state fermentation medium) of the compound fermentation seed liquid, mix well, and ferment at 35°C for 24 hours to complete the first stage of fermentation. Adjust the fermentation temperature to 28°C to begin the second stage of fermentation. After 20-24 hours of the second stage fermentation, measure the concentration of the mixed bacteria; the concentration should not be less than 5 × 10⁻⁶. 10 CFU / g, and the fermentation product was collected after fermentation was completed.
[0043] The above fermentation products were freeze-dried, then granulated into fine particles using a granulator and packaged to obtain 4×10⁴ granules. 10 Functional feed with CFU / g microbial agent, labeled as #1 functional feed.
[0044] Example 4
[0045] This embodiment describes the preparation of functional feed using the above-ground stems and leaves of Alpinia galanga as substrate and co-fermentation with Bacillus licheniformis CICC 10103 and Bacillus subtilis CGMCC 1.9086.
[0046] Preparation of solid-state fermentation culture medium:
[0047] The aerial stems and leaves of Alpinia galanga were dried to constant weight in a drying oven at 60℃, and then pulverized through a 100-mesh sieve to obtain Alpinia galanga aerial stem and leaf powder. Wheat bran was subjected to high-pressure steam treatment at 170℃ for 5 minutes, and then dried to constant weight in a drying oven at 60℃. Wheat bran was sieved through a 100-mesh sieve to obtain wheat bran powder. The Alpinia galanga aerial stem and leaf powder and wheat bran powder were mixed at a mass ratio of 5:5, and 1% yeast extract, 0.05% MgSO4, 0.01% MnSO4 and 0.005% CuSO4 were added and mixed evenly to obtain a solid-state fermentation medium.
[0048] The seed culture of Bacillus licheniformis CICC 10103 prepared in Example 1 and the seed culture of Bacillus subtilis CGMCC 1.9086 prepared in Example 2 were mixed at a volume ratio of 1:1 to obtain a compound fermentation seed culture.
[0049] Add solid-state fermentation medium and an equal volume of water, mix well, then add 4% (by weight of total solid-state fermentation medium) of the compound fermentation seed liquid, mix well, and ferment at 34℃ for 24 hours to complete the first stage of fermentation. Adjust the fermentation temperature to 28℃ to begin the second stage of fermentation. After 20-24 hours of the second stage fermentation, measure the concentration of the mixed bacteria; the concentration should not be less than 5 × 10⁻⁶. 10 CFU / g, and the fermentation product was collected after fermentation was completed.
[0050] After drying, the fermentation products were granulated into fine particles using a granulator and then packaged to obtain 4×10⁴ granules. 10 This functional feed, with a CFU / g microbial agent content, is labeled as #2 functional feed.
[0051] Example 5
[0052] This embodiment describes the preparation of functional feed using the above-ground stems and leaves of Alpinia galanga as substrate and co-fermentation with Bacillus licheniformis CICC 10103 and Bacillus subtilis CGMCC 1.9086.
[0053] Preparation of solid-state fermentation culture medium:
[0054] The aerial stems and leaves of Alpinia galanga were dried to constant weight in a drying oven at 60℃, and then pulverized through a 100-mesh sieve to obtain Alpinia galanga aerial stem and leaf powder. Wheat bran was subjected to high-pressure steam treatment at 180℃ for 5 minutes, and then dried to constant weight in a drying oven at 60℃. Wheat bran was sieved through a 100-mesh sieve to obtain wheat bran powder. The Alpinia galanga aerial stem and leaf powder and wheat bran powder were mixed at a mass ratio of 4:6, and 1% yeast extract, 0.05% MgSO4, 0.01% MnSO4 and 0.005% CuSO4 were added and mixed evenly to obtain a solid-state fermentation medium.
[0055] The seed culture of Bacillus licheniformis CICC 10103 prepared in Example 1 and the seed culture of Bacillus subtilis CGMCC 1.9086 prepared in Example 2 were mixed at a volume ratio of 1:2 to obtain a compound fermentation seed culture.
[0056] Add solid-state fermentation medium and an equal amount of water, mix well, then add 5% (by weight of total solid-state fermentation medium) of the compound fermentation seed liquid, mix well, and ferment at 36°C for 24 hours to complete the first stage of fermentation. Adjust the fermentation temperature to 28°C to begin the second stage of fermentation. After 20-24 hours of the second stage fermentation, measure the concentration of the mixed bacteria; the concentration should not be less than 5 × 10⁻⁶. 10 CFU / g, and the fermentation product was collected after fermentation was completed.
[0057] After drying the above fermentation products, starch was added and the mixture was granulated into fine granules using a granulator and then packaged to obtain 4×10⁴ granules. 10 This functional feed, with a CFU / g microbial agent content, is labeled as #3 functional feed.
[0058] Comparative Example 1
[0059] The only difference between this comparative example and Example 5 is that it uses a single strain of Bacillus licheniformis CICC 10103 for fermentation.
[0060] Preparation of solid-state fermentation culture medium:
[0061] The aerial stems and leaves of Alpinia galanga were dried to constant weight in a drying oven at 60℃, and then pulverized through a 100-mesh sieve to obtain Alpinia galanga aerial stem and leaf powder. Wheat bran was subjected to high-pressure steam treatment at 180℃ for 5 minutes, and then dried to constant weight in a drying oven at 60℃. Wheat bran was sieved through a 100-mesh sieve to obtain wheat bran powder. The Alpinia galanga aerial stem and leaf powder and wheat bran powder were mixed at a mass ratio of 4:6, and 1% yeast extract, 0.05% MgSO4, 0.01% MnSO4 and 0.005% CuSO4 were added and mixed evenly to obtain a solid-state fermentation medium.
[0062] Add solid-state fermentation medium and an equal volume of water, mix well, then add 5% (by weight of total solid-state fermentation medium) of Bacillus licheniformis CICC 10103 seed culture, mix well, and ferment at 36°C for 24 hours to complete the first stage of fermentation. Adjust the fermentation temperature to 28°C to begin the second stage of fermentation. After 20-24 hours of the second stage fermentation, determine the bacterial concentration; the concentration should not be less than 5 × 10⁻⁶. 10 CFU / g, and the fermentation product was collected after fermentation was completed.
[0063] After drying, the fermentation products were granulated into fine particles using a granulator and then packaged to obtain 4×10⁴ granules. 10Functional feed with CFU / g microbial agent, labeled as #1 functional feed contrast agent.
[0064] Comparative Example 2
[0065] The only difference between this comparative example and Example 5 is that it uses a single strain of Bacillus subtilis CGMCC 1.9086 for fermentation.
[0066] Preparation of solid-state fermentation culture medium:
[0067] The aerial stems and leaves of Alpinia galanga were dried to constant weight in a drying oven at 60℃, and then pulverized through a 100-mesh sieve to obtain Alpinia galanga aerial stem and leaf powder. Wheat bran was subjected to high-pressure steam treatment at 180℃ for 5 minutes, and then dried to constant weight in a drying oven at 60℃. Wheat bran was sieved through a 100-mesh sieve to obtain wheat bran powder. The Alpinia galanga aerial stem and leaf powder and wheat bran powder were mixed at a mass ratio of 4:6, and 1% yeast extract, 0.05% MgSO4, 0.01% MnSO4 and 0.005% CuSO4 were added and mixed evenly to obtain a solid-state fermentation medium.
[0068] Add solid-state fermentation medium and an equal volume of water, mix well, then add 5% (by weight of the total solid-state fermentation medium) of Bacillus subtilis CGMCC 1.9086 seed culture, mix well, and ferment at 36°C for 24 hours to complete the first stage of fermentation. Adjust the fermentation temperature to 28°C to begin the second stage of fermentation. After 20-24 hours of the second stage fermentation, measure the bacterial concentration; the concentration should not be lower than 5 × 10⁻⁶. 10 CFU / g, and the fermentation product was collected after fermentation was completed.
[0069] After drying, the fermentation products were granulated into fine particles using a granulator and then packaged to obtain 4×10⁴ granules. 10 Functional feed with CFU / g microbial agent, labeled as #2 functional feed contrast agent.
[0070] Comparative Example 3
[0071] This comparative example uses a functional feed contrast agent prepared from unfermented raw materials.
[0072] The aerial stems and leaves of Alpinia galanga were dried to constant weight in a 60℃ drying oven, then pulverized and passed through a 100-mesh sieve to obtain Alpinia galanga aerial stem and leaf powder. Wheat bran was subjected to high-pressure steam treatment at 180℃ for 5 minutes, followed by drying to constant weight in a 60℃ drying oven. The wheat bran was then passed through a 100-mesh sieve to obtain wheat bran powder. The Alpinia galanga aerial stem and leaf powder and wheat bran powder were mixed at a mass ratio of 4:6, granulated into fine granules using a pellet mill, and then packaged. This mixture was labeled as #3 functional feed contrast agent.
[0073] Example 6
[0074] This embodiment tests the effects of different treatments on the growth performance and diarrhea rate of piglets by adding functional feeds prepared by co-fermentation of two strains from Examples 3-5, functional feeds prepared by single-strain fermentation from Comparative Examples 1 and 2, and unfermented functional feed #3.
[0075] Experimental protocol: Seventy healthy piglets weaned at 21 days of age were randomly divided into 7 groups of 10 piglets each, with an experimental period of 14 days. The experiment consisted of 7 groups: one blank control group, 3 experimental groups, and 3 control groups. The blank control group piglets were fed a basal diet; the experimental group #1 piglets were fed a basal diet supplemented with functional feed #1, with a final bacterial concentration of 1.0 × 10⁻⁶. 8 cfu / g; Piglets in experimental group #2 were fed a basal diet supplemented with functional feed #2, with a final bacterial concentration of 1.0 × 10⁻⁶. 8 cfu / g; Piglets in experimental group #3 were fed a basal diet supplemented with functional feed #3, with a final bacterial concentration of 1.0 × 10⁻⁶. 8 cfu / g; Piglets in control group #1 were fed a basal diet supplemented with functional feed contrast agent #1, with a final bacterial concentration of 1.0 × 10⁻⁶. 8 CFU / g, control group piglets were fed a basal diet supplemented with functional feed additive #2, with a final bacterial concentration of 1.0 × 10⁻⁶ CFU / g. 8 cfu / g; Piglets in control group #3 were fed a basal diet supplemented with functional feed contrast agent #3, at the same amount as functional feed contrast agent #2.
[0076] Piglet weight was recorded on days 0 and 14 of the experiment, along with total feed intake and diarrhea status. At the end of the experiment, daily weight gain, daily feed intake, feed conversion ratio, and diarrhea index were calculated.
[0077] Average daily weight gain (g / d) = (final weight at the end of the experiment - initial weight at the beginning of the experiment) / number of days in the experiment;
[0078] Average daily feed intake (g / d) = Total feed intake per head / Number of days in the experiment;
[0079] Feed conversion ratio = Average daily feed intake / Average daily weight gain;
[0080] Diarrhea Index = Sum of diarrhea scores for each group of piglets / (Total number of piglets × Total number of days).
[0081] The diarrhea scoring criteria are as follows: dry feces, 1 point; no diarrhea, normal dry feces, 2 points; mild diarrhea, feces slightly moist, semi-dry feces, 3 points; moderate diarrhea, soft feces, slightly loose, 4 points; severe diarrhea, watery feces, 5 points. A score of 3 or higher is defined as diarrhea. The incidence of diarrhea in piglets in each group was calculated during the experiment.
[0082] Diarrhea rate = (Number of diarrheal animals × Number of days with diarrhea) / (Total number of animals in each group × Total number of days in the trial)
[0083] Table 1. Growth of piglets after adding different functional feeds
[0084]
[0085] Table 2. Statistical results of diarrhea in piglets after adding different functional feeds
[0086]
[0087] Table 1 shows that, compared with the control group, and with the control group (Alpinia galanga aerial stems and leaves) and the control group (Bacillus licheniformis CICC10103 and Bacillus subtilis CGMCC 1.9086), the functional feeds (1#-3#) prepared by co-fermentation of Bacillus licheniformis CICC10103 and Bacillus subtilis CGMCC 1.9086) significantly promoted piglet feed intake and growth. This suggests a synergistic effect of the metabolites produced by the co-fermentation of the two strains. The functional feeds prepared by single-strain fermentation in the control groups (1# and 2#) also promoted piglet growth compared with the control group, but their growth-promoting effect was much weaker than that of the co-fermentation groups. The unfermented functional feed in the control group (3#) had no growth-promoting effect on piglets, and the piglets' growth was even worse than that of the control group.
[0088] Table 2 shows that, compared with the control group (3#), the unfermented functional feed alone reduced the diarrhea rate and significantly decreased the diarrhea index in piglets, indicating that although galangal does not promote piglet growth, it significantly reduces the severity and rate of diarrhea. Compared with the control group (3#), the single-strain galangal fermentation group (2# and 1#) further reduced the diarrhea rate and severity in piglets, with Bacillus licheniformis showing a slightly better effect on the diarrhea index than Bacillus subtilis. Compared with the blank control group and the control group (1#-3#), the diarrhea rate and diarrhea index in piglets decreased significantly in the experimental groups (1#-3# and 1#-3#), demonstrating a synergistic effect of galangal fermentation and the co-fermentation of Bacillus licheniformis CICC 10103 and Bacillus subtilis CGMCC 1.9086.
[0089] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention should be included within the protection scope of the present invention.
Claims
1. A method for preventing and treating diarrhea in piglets, characterized in that, Includes the following steps: Feeding piglets with functional feed, wherein the method for preparing the functional feed includes: S1. Preparation of Bacillus licheniformis Bacillus licheniformis The seed liquid of the Bacillus licheniformis Bacillus licheniformis Its accession number is CICC 10103; S2, Preparation of Bacillus subtilis Bacillus subtilis Seed liquid of Bacillus subtilis Bacillus subtilis Its accession number is CGMCC 1.9086; S3. The Bacillus licheniformis prepared in step S1 Bacillus licheniformis The seed liquid and Bacillus subtilis prepared in step S2 Bacillus subtilis The seed liquids were mixed at a volume ratio of 1-2:1-2 to obtain a compound fermentation seed liquid; S4. Prepare a solid-state fermentation medium, wherein the solid-state fermentation medium comprises galangal aerial stem and leaf powder and wheat bran powder, wherein the mass ratio of galangal aerial stem and leaf powder to wheat bran powder is 4-7:6-3. S5. Add water to the solid fermentation medium in step S4, the amount of water being equal to the mass of the solid fermentation medium, and then add 3%-5% of the total mass of the solid fermentation medium to the compound fermentation seed liquid prepared in step S3, and mix well. S6. Perform two-stage fermentation: the first stage is fermented at 34-36℃ for 24 hours, and the second stage is fermented at 28℃ for 20-24 hours to obtain the fermentation product; S7. The fermentation product obtained in step S6 is dried and granulated to obtain the functional feed.
2. The method according to claim 1, characterized in that, In step S3, the mass ratio of the Bacillus licheniformis seed liquid to the Bacillus subtilis seed liquid is 1:
1.
3. The method according to claim 2, characterized in that, In step S4, the mass ratio of the galangal above-ground stem and leaf powder to the wheat bran powder is 5:
5.
4. The method according to claim 3, characterized in that, In step S5, 5% of the total mass of the solid fermentation medium is added to the compound fermentation seed liquid prepared in step S3.
5. The method according to claim 4, characterized in that, In step S6, the first stage ferments at 35°C for 24 hours.
6. A functional feed, characterized in that, The functional feed is prepared by the method described in any one of claims 1-5.
7. The functional feed according to claim 6, characterized in that, It contains fermented galangal stem and leaf powder and wheat bran powder, as well as Bacillus licheniformis. Bacillus licheniformis and Bacillus subtilis Bacillus subtilis Live bacteria and / or their metabolites.
8. The application of the aerial stems and leaves of Alpinia galanga in the preparation of feed for the prevention and / or treatment of diarrhea in piglets, characterized in that, The above-ground stems and leaves of Alpinia galanga are treated with Bacillus licheniformis. Bacillus licheniformis and Bacillus subtilis Bacillus subtilis Co-fermentation treatment.