Light source multiplexing diamond NV color center wide field detection system and illumination detection method

Abstract

The invention provides a light source multiplexing diamond NV color center wide field detection system and an illumination detection method. The system comprises a diamond containing an NV color center, an exciting light module, a first double-color sheet, an objective lens, a second double-color sheet, a wide field imaging module, an illumination imaging module and a microwave module, exciting light generated by the exciting light module irradiates the first double-color piece, is guided to the objective lens, and irradiates the diamond after being transmitted by the objective lens so as to excite the NV color center of the diamond to generate fluorescent light; the objective lens is of an infinity type; the exciting light is irradiated to a sample to be detected after being transmitted by the diamond; fluorescent light and exciting light from a sample to be detected are collected by the objective lens, then transmitted to the first double-color piece and guided to the second double-color piece through the first double-color piece, the second double-color piece guides the fluorescent light to the wide-field imaging module for imaging, and the exciting light in the second double-color piece is guided to the illumination imaging module for imaging. Illumination can be achieved in the exciting light working state, exciting light switching operation in the prior art is avoided, and efficiency can be remarkably improved.

Description

Technical Field

[0001] This invention relates to the field of quantum measurement technology, and in particular to a diamond NV color center wide-field detection system and illumination detection method that utilizes a multiplexed light source. Background Technology

[0002] Existing wide-field diamond NV color center detection systems use a long-pass filter in front of the lens to filter out red fluorescence, resulting in weak object-side illumination in non-operating states. This leads to insufficient clarity of the object-side observation on the image side, making it impossible to determine the state of the object under test. Existing technologies employ red light illumination to supplement the object-side illumination (see patent application CN202323228797.4). However, in this system, the illumination system can only be used in non-operating states, i.e., the illumination light is turned on for illumination detection when the excitation light is off. For situations requiring illumination observation in operating states, such as wide-field imaging of multiple regions of the sample under test, where the sample needs to be moved from one region to another after imaging in another, existing technologies can only turn off the excitation light and then turn on the illumination light each time the sample needs to be moved to observe the designated detection area. This results in numerous operational steps, and for some excitation lights, there is a waiting period after restarting to allow them to return to a stable state, which obviously reduces efficiency. Furthermore, the multiple switching operations of the excitation light affect its performance. Summary of the Invention

[0003] In view of the shortcomings of the prior art described above, the purpose of this invention is to provide a diamond NV color center wide-field detection system and illumination detection method with multiplexed light source, which solves the problem that the illumination in the wide-field detection of the prior art can only be used in the non-working state and cannot meet the needs of illumination observation in the working state.

[0004] To achieve the above and other related objectives, a first aspect of the present invention provides a wide-field detection system for diamond NV color centers using a multiplexed light source, comprising: a diamond containing NV color centers, an excitation light module, a first dichroic film, an objective lens, a second dichroic film, a wide-field imaging module, an illumination imaging module, and a microwave module; excitation light generated by the excitation light module irradiates the first dichroic film and is guided to the objective lens, and after transmission through the objective lens, irradiates the diamond to excite the diamond NV color centers to generate fluorescence; the objective lens is infinity-type; the excitation light is also transmitted through the diamond to irradiate the sample to be tested; the fluorescence generated by the diamond and the excitation light from the sample to be tested are collected by the objective lens and transmitted to the first dichroic film, and guided by the first dichroic film to the second dichroic film, the second dichroic film guides the fluorescence therein to the wide-field imaging module for imaging, and guides the excitation light therein to the illumination imaging module for imaging; the microwave module is used to radiate microwaves to the diamond.

[0005] Furthermore, the first dichroic filter is an interference-type long-pass or short-pass dichroic filter with a cutoff wavelength λ1 of 532nm < λ1 < 610nm, and the first dichroic filter satisfies: It ranges from 0.5% to 10%, of which, The first dichroic filter directs the excitation light to the second dichroic filter, The incident light power is the excitation light transmitted from the objective lens to the first dichroic plate.

[0006] Furthermore, the second dichroic filter is an interference-type long-pass or short-pass dichroic filter with a cutoff wavelength λ2 of 532nm < λ2 < 637nm, and the second dichroic filter satisfies: It is 0-0.5%, of which, To excite the light to be directed to the wide-field imaging module, The light power that directs the excitation light from the first dichroic filter to the second dichroic filter.

[0007] Furthermore, the excitation light module includes a laser and a first condenser lens, the first condenser lens being used to converge and shape the laser beam emitted from the excitation light to obtain a beam size that meets the requirements for entering the objective lens.

[0008] Furthermore, the excitation light module also includes frosted glass, located between the first condenser lens and the first dichroic filter.

[0009] Furthermore, the excitation light module includes a light-emitting diode and one or more focusing lenses, wherein the focusing lenses are used to converge and collimate the light emitted from the light-emitting diode to reduce the divergence angle of the light.

[0010] Furthermore, the wide-field imaging module includes a first filter, a first imaging lens, and a first imaging camera arranged sequentially along the light collection direction; the illumination imaging module includes a second imaging lens and a second imaging camera arranged sequentially along the light collection direction.

[0011] Furthermore, it also includes a bias magnetic field module for applying a bias magnetic field to the probe.

[0012] Furthermore, it also includes a moving device, on which the sample to be tested is placed on a platform and the movement of the sample to be tested can be controlled; and a data processing module, connected to the wide-field imaging module and the illumination imaging module, for reading fluorescence imaging data and illumination imaging images respectively, and processing and analyzing them, and connected to the microwave module for controlling the microwave frequency radiated by it.

[0013] To achieve the above and other related objectives, a second aspect of the present invention provides an illumination detection method for synchronous wide-field imaging, employing a diamond NV color center wide-field detection system with sustainable illumination as described in any one of the first aspects. The method includes: placing the sample to be tested below the diamond, illuminating it with the excitation light from the excitation light module, imaging the fluorescence with the wide-field imaging module, and imaging the excitation light used for illumination with the illumination imaging module; when measuring the magnetic field of the sample to be tested, performing ODMR measurement and reading the fluorescence imaging data output by the wide-field imaging module, and obtaining the magnetic field intensity distribution based on this data; when it is necessary to observe the sample to be tested, reading the illumination imaging image output by the illumination imaging module, which is the imaging image of the sample surface.

[0014] As described above, the diamond NV color center wide-field detection system and illumination detection method of the present invention, which reuses the light source, has the following beneficial effects: It uses the excitation light generated by the excitation light module as the light source for NV color center excitation and objective lens object-side illumination, eliminating the need for additional illumination light, thus saving costs and simplifying the system structure. The excitation light is guided to the objective lens through a first dichroic filter. When the fluorescence collected by the objective lens and the excitation light are transmitted to the first dichroic filter, if the excitation light is located in the transition zone, a portion of the incident excitation light can be reflected back to the objective lens for excitation. When the fluorescence collected by the objective lens and the excitation light are transmitted to the first dichroic filter, in addition to the fluorescence with wavelengths greater than the cutoff wavelength being transmitted, a portion of the excitation light is transmitted through transmission to the second dichroic filter. Since the wavelengths of the excitation light and fluorescence are different, the second dichroic filter separates the two types of light through transmission and reflection. The separated excitation light enters the illumination imaging module for imaging, and the fluorescence enters the wide-field imaging module for imaging. Illumination can be achieved while the excitation light is in operation, avoiding the excitation light switching operation in the prior art and significantly improving efficiency. Attached Figure Description

[0015] Figure 1 The diagram shown is a schematic of the first structure of a wide-field detection system. Figure 2 The diagram shows the spectral transmittance curves of a two-color film. Figure 3 This is a schematic diagram of the second structure of a wide-field detection system; Figure 4 The image shows a comparison of the lighting before and after adding frosted glass; Figure 5 This is a schematic diagram of the third structure of a wide-field detection system; Figure 6 The diagram shows the structure of a TIR lens. Figure 7 This is a schematic diagram of the fourth structure of a wide-field detection system; Figure 8 The image shows intensity angle slices of incoherent light without and with a TIR lens.

[0016] Component labeling: 1—Diamond; 2—Excitation light module; 21—Laser; 22—First condenser lens; 23—Light emission diode; 24—TIR lens; 25—Convex lens; 26—Frosted glass; 3—First dichroic filter; 4—Objective lens; 5—Second dichroic filter; 6—Wide-field imaging module; 61—First filter; 62—First imaging lens; 63—First imaging camera; 7—Illumination imaging module; 71—Second imaging lens; 72—Second imaging camera; 73—Second filter; 8—Microwave module; 81—Microwave generation module; 82—Microwave antenna; 9—Magnet; 10—Sample under test; 20—Moving device; 30—Data processing module. Detailed Implementation

[0017] The following specific examples illustrate the implementation of the present invention. Those skilled in the art can easily understand other advantages and effects of the present invention from the content disclosed in this specification. The present invention can also be implemented or applied through other different specific embodiments, and various details in this specification can also be modified or changed based on different viewpoints and applications without departing from the spirit of the present invention. It should be noted that, unless otherwise specified, the following embodiments and features described therein can be combined with each other.

[0018] It should be noted that the illustrations provided in the following embodiments are only schematic representations of the basic concept of the present invention. Therefore, the illustrations only show the components related to the present invention and are not drawn according to the actual number, shape and size of the components in the actual implementation. In the actual implementation, the form, quantity and proportion of each component can be arbitrarily changed, and the layout of the components may also be more complex.

[0019] Example 1: As Figure 1 As shown, this embodiment provides a diamond NV color center wide-field detection system with multiplexed light source, including: a diamond containing NV color centers 1, an excitation light module 2, a first dichroic filter 3, an objective lens 4, a second dichroic filter 5, a wide-field imaging module 6, an illumination imaging module 7, and a microwave module 8. The excitation light generated by the excitation light module 2 illuminates the first dichroic film 3 and is guided to the objective lens 4. After being transmitted through the objective lens 4, it illuminates the diamond 1 to excite the NV color center of the diamond to produce fluorescence. The objective lens 4 is infinity-type. The excitation light is also transmitted through the diamond 1 and illuminates the sample 10 to be tested. The fluorescence generated by the diamond 1 and the excitation light from the sample to be tested are collected by the objective lens 4 and transmitted to the first dichroic film 3. The first dichroic film 3 guides the fluorescence to the second dichroic film 5. The second dichroic film 5 guides the fluorescence to the wide field imaging module 6 for imaging and the excitation light to the illumination imaging module 7 for imaging. Microwave module 8 is used to radiate microwaves to the diamond.

[0020] In this embodiment, the excitation light generated by the excitation light module is used as the light source for NV color center excitation and objective lens object-side illumination, eliminating the need for additional illumination light. This saves costs and simplifies the system structure. The excitation light is guided to the objective lens through the first dichroic filter. When the fluorescence collected by the objective lens and the excitation light are transmitted to the first dichroic filter, the cutoff wavelength on the long-pass or short-pass spectral transmittance curve is located at the center of the transition band of the curve. Figure 2 As shown by λ0, taking the long-pass type as an example, the light located in the transition zone is less than the maximum transmittance. When the excitation light is located in the transition zone in the first dichroic filter, a portion of the excitation light incident on it can be reflected to the objective lens for excitation. When the fluorescence and excitation light collected by the objective lens are transmitted to the first dichroic filter, in addition to the fluorescence with wavelengths greater than the cutoff wavelength being transmitted, a portion of the excitation light is transmitted through transmission to the second dichroic filter. Since the wavelengths of the excitation light and fluorescence are different, the second dichroic filter can separate the two types of light through transmission and reflection. The separated excitation light enters the illumination imaging module for imaging, and the fluorescence enters the wide-field imaging module for imaging. Thus, this embodiment can achieve illumination while the excitation light is in operation, avoiding the excitation light switching operation in the prior art, which can significantly improve efficiency, and the excitation light source can be reused, saving cost and structural space.

[0021] Since the excitation wavelength of the diamond NV color center is 532nm, the wavelength of the fluorescence generated by the excitation is 600nm-800nm, with most wavelengths in the 637nm-800nm ​​range. The first dichroic plate 3 is an interference-type long-pass or short-pass dichroic plate with a cutoff wavelength λ1 of 532nm < λ1 < 610nm, and the first dichroic plate satisfies: It ranges from 0.5% to 10%, of which, The first dichroic filter directs the excitation light to the second dichroic filter, This represents the incident light power of the excitation light transmitted from the objective lens to the first dichroic plate. The second dichroic plate 5 is an interference-type long-pass or short-pass dichroic plate with a cutoff wavelength λ2 of 532nm < λ2 < 637nm, and the excitation light is guided to the wide-field imaging module 6 by the light power. and The smaller the ratio, the better; preferred. It is 0-0.5%. For example... Figure 1Both dichroic filters shown are long-pass type. The first dichroic filter 3 reflects the excitation light from the excitation module to the objective lens, while transmitting the fluorescence and excitation light from the objective lens to the second dichroic filter 5. The second dichroic filter 5 reflects the excitation light to the illumination imaging module 7 and transmits the fluorescence to the wide-field imaging module 6. Of course, the transmitted light may also include a small amount of excitation light. An absorptive long-pass filter is added to the wide-field imaging module, such as... Figure 1 The first filter 61 shown has a cutoff wavelength λ3 of 600nm < λ3 < 637nm to further filter out the excitation light. Alternatively, it can be as follows... Figure 5 As shown, a second filter 73 is added to the illumination imaging module 7 to filter out the excitation light for illumination imaging. An absorption-type low-pass filter with a cutoff wavelength λ4 of 532nm < λ4 < 600nm or a band-pass filter that only allows the excitation light to pass through can be selected.

[0022] In this embodiment, the excitation light generated by the excitation light module 2 is a laser, for example as follows: Figure 1 As shown, the excitation light module 2 includes a laser 21 and a first condenser lens 22. The 532nm laser generated by the laser 21 is focused by the first condenser lens 22 and then transmitted to the first dichroic filter 3. The first condenser lens 22 converges and shapes the beam to obtain a beam size that meets the requirements of the subsequent objective lens, and works with the objective lens to achieve wide-field imaging. The first condenser lens 22 can be a biconvex lens. The excitation beam output by the laser 21 is a coarse beam with a beam diameter of 10mm-25mm to meet the wide-field requirement.

[0023] The wide-field imaging module 6 includes a first filter 61, a first imaging lens 62, and a first imaging camera 63 arranged sequentially along the light collection direction. The illumination imaging module 7 includes a second imaging lens 71 and a second imaging camera 72 arranged sequentially along the light collection direction. The first imaging lens 62 and the second imaging lens 71 are both FA lenses or tube lenses, and the first imaging camera 63 and the second imaging camera 72 are both CCD or CMOS cameras.

[0024] In this embodiment, the first imaging lens 62 can be further configured as a fixed-focus lens, and the second imaging lens 71 as a zoom lens. The fixed-focus lens is used for wide-field imaging to ensure image quality, while the zoom lens is used for illumination imaging and can adjust the imaging field of view as needed to facilitate observation for different purposes. When a low-magnification objective lens is selected, such as 5x magnification, the detection field of view of the objective lens can be larger than the area of ​​the diamond. In this case, the fixed-focus lens can be used to focus only on the field of view of the diamond surface, while the zoom lens can be used to change the focus on the diamond surface and the field of view around the diamond.

[0025] The microwave module 8 includes a microwave generating module 81 and a microwave antenna 82. The microwave generating module 81 may, exemplarily, include a microwave source, a microwave switch, a microwave amplifier, and a microwave circulator connected in sequence. The microwaves generated by the microwave source are transmitted to the microwave antenna 82 and then radiated to the diamond 1 by the microwave antenna 82. In this embodiment, the diamond 1 has a sheet-like structure with dimensions of tens or hundreds of micrometers or millimeters, enabling wide-field imaging. The diamond is transparent, and light irradiated onto the diamond can be transmitted to the sample. The microwave antenna 82 can be a microstrip antenna, such as a coplanar waveguide. The diamond is located in or below the central aperture of the coplanar waveguide, and light irradiates the diamond through the central aperture.

[0026] In this embodiment, in some cases, such as when the direction of the magnetic field to be measured is known, the desired ODMR spectral line can be obtained by adjusting the angle between the magnetic field direction and the axis of the color center. In other cases, such as when the direction of the magnetic field to be measured is unknown or when detecting weak magnetic fields, a bias magnetic field module that applies a bias magnetic field to the diamond can be further added. This is used to adjust the resonance peak generated by the color center, or to make the detection of weak magnetic fields by the color center operate in the linear region, or to adjust the detection frequency band to improve the sensitivity of weak magnetic field detection. The bias magnetic field module includes, for example, a Helmholtz coil or such as... Figure 3 Magnet 9 is shown.

[0027] Example 2: Based on Example 1, as follows Figure 3 As shown, in this embodiment, a frosted glass 26 is added to the excitation light module, located between the convex lens 22 and the first dichroic film 3. By scattering light, it can be used to reduce the generation of interference fringes caused by coherent light.

[0028] like Figure 4 As shown, Figure a is the image without frosted glass 26, where interference fringes are visible. Figure b is the image after adding frosted glass 26, where the interference fringes are basically eliminated.

[0029] Example 3: Based on Example 1, this example differs from Example 1 in that the excitation light generated by the excitation light module is incoherent light, as exemplified by... Figure 5 As shown, the excitation light module includes a light-emitting diode (LED) 23 and one or more condenser lenses. The condenser lenses are used to converge and collimate the light emitted from the LED to reduce the divergence angle. The excitation light generated by the LED 23 is incoherent light, which, compared to highly coherent laser light, allows for better uniformity of the generated light spot and avoids the generation of interference fringes in imaging. The condenser lens can be a TIR lens (total internal reflection lens). Figure 5 The example demonstrates the use of a TIR lens 24 to focus and collimate the incoherent light emitted from the LED 23, thereby reducing the divergence angle and increasing the light intensity. For example... Figure 6As shown, the TIR lens has seven transmission surfaces: 241, 242, 243, 244, 245, 246, and 247. Surfaces 241, 244, and 247 are Bezier surfaces, while the others are flat surfaces. Light rays at small angles pass through transmission surfaces 241 and 247, while light rays at large angles pass through transmission surfaces 242, 244, and 246. Surface 244 is a total internal reflection surface. This allows for the collimation and limitation of the divergence angle of both large and small-angle light. Figure 8 As shown, the divergence angle in image b after using a TIR lens is much smaller than that in image a without a TIR lens, and the light intensity in image b is significantly increased. The divergence angle after using a TIR lens is 4-10 degrees, which meets the requirements for subsequent imaging.

[0030] Figure 7 The example provided illustrates a combination of a TIR lens and a convex lens 25. The convex lens 25 converges the collimated beam emitted from the TIR lens, which, when combined with a subsequent objective lens, increases the spot size on the object-side imaging plane of the objective lens to meet wide-field imaging requirements. The convex lens 25 can be a plano-convex lens or a biconvex lens. Alternatively, the TIR lens can be combined with a hemispherical lens.

[0031] Example 4: Based on Example 1, Example 2, or Example 3, such as... Figure 7 As shown, it also includes a moving device 20, on which the sample to be tested 10 is placed and the sample to be tested 10 can be moved; and a data processing module 30, connected to the wide-field imaging module 6 and the illumination imaging module 7, for reading fluorescence imaging data and illumination imaging images respectively and processing and analyzing them, and connected to the microwave module 8 for controlling the microwave frequency radiated by it.

[0032] The principle of measuring magnetic fields using diamond NV centers is to utilize the Zeeman splitting effect of the ground state energy level of the NV center caused by an external magnetic field, then detect the frequency shift of the resonant point of the ODMR spectrum, and finally convert the frequency shift into magnetic field strength using the spin-magnetic ratio of the NV center electrons, thereby achieving the purpose of detecting the external magnetic field.

[0033] The use of the wide-field detection system in this embodiment to perform ODMR measurements to obtain the magnetic field strength distribution refers to performing fluorescence imaging for each frequency point during ODMR using the microwave frequency sweep method, obtaining a grayscale image, acquiring imaging data, plotting the ODMR spectrum of the grayscale value of each pixel as a function of microwave frequency, and then calculating the magnetic field strength of each pixel from the resonance frequency on the spectrum. These are then combined into a single image to form the magnetic field strength distribution. The illumination imaging image can reflect the characteristic information of the sample surface and can be used to observe whether the area within the field of view is the target region. The image output by the illumination imaging module can be read as needed. For example, in the initial stage of wide-field detection, illumination imaging can be used to observe whether the position of the placed sample ensures that the target region is within the detection field of view; or after performing ODMR measurements on one detection area of ​​the sample, the sample can be moved to ensure that other areas are within the detection field of view, and illumination imaging can be used to observe whether the target region is within the detection field of view; or after performing ODMR, the obtained magnetic field strength distribution needs to be verified against the target region on the sample.

[0034] Example 5: This example provides a synchronous wide-field imaging illumination detection method, using the diamond NV color center wide-field detection system with multiplexed light sources as described in any of the preceding examples. The method includes: placing the sample 10 to be tested below the diamond 1, illuminating it with the excitation light from the excitation light module 2, imaging the fluorescence with the wide-field imaging module 6, and imaging the excitation light used for illumination with the illumination imaging module 7; when measuring the magnetic field of the sample 10, performing ODMR measurement and reading the fluorescence imaging data output by the wide-field imaging module 6 to obtain the magnetic field intensity distribution; when observation of the sample is required, reading the illumination imaging image output by the illumination imaging module 7, which is the image of the sample surface.

[0035] When using the diamond NV color center wide-field detection system with light source reuse in Example 4, it can be applied to situations where wide-field imaging of multiple regions of a sample is performed. Therefore, the illumination detection method for synchronous wide-field imaging includes: S1, the sample to be tested 10 is placed below the diamond 1, the position of the sample to be tested 10 is adjusted by the moving device 20, the excitation light of the excitation light module 2 is turned on, the fluorescence is imaged by the wide field imaging module 6, and the excitation light for illumination is imaged by the illumination imaging module 7. S2, the data processing module 30 reads the illumination imaging image output by the illumination imaging module 7, analyzes and judges the imaging image. If it is the target area, ODMR measurement is performed on the target area, and the data processing module 30 reads the fluorescence imaging data output by the wide field imaging module 6. Based on this data, the magnetic field intensity distribution is obtained, and then step S4 is executed; if it is not the target area, step S3 is executed. S3, the position of the sample to be tested is adjusted by the moving device 20, and step S2 is executed; S4. If there are still target areas that need to be detected, proceed to step S3; if there are no target areas that need to be detected, end the measurement.

[0036] The target area mentioned above is known in advance and can be defined by specific coordinates. The moving device can be a three-dimensional precision adjustment device. When the moving device 20 adjusts the position of the sample to be tested, the three-dimensional displacement of the movement is also set according to the coordinates of the area. However, whether the position adjustment according to the theoretical design can ensure that the required target area is under the imaging field of view needs to be calibrated before wide field imaging. In this embodiment, after the position of the sample to be tested is adjusted, the illumination detection further verifies and calibrates whether the target area is under the imaging field of view to avoid measurement errors caused by operational errors or inaccurate pre-designed movement displacement, thereby ensuring the accuracy of the final magnetic field measurement.

[0037] The above embodiments are merely illustrative of the principles and effects of the present invention and are not intended to limit the invention. Any person skilled in the art can modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by those skilled in the art without departing from the spirit and technical concept disclosed in the present invention should still be covered by the claims of the present invention.

Claims

1. A wide-field detection system for diamond NV color centers using multiplexed light sources, characterized in that, The detection system includes: a diamond containing NV color centers, an excitation light module, a first dichroic filter, an objective lens, a second dichroic filter, a wide-field imaging module, an illumination imaging module, and a microwave module; The excitation light generated by the excitation light module illuminates the first dichroic film and is guided to the objective lens. After being transmitted through the objective lens, it illuminates the diamond to excite the NV color center of the diamond to produce fluorescence. The objective lens is infinity-type. The excitation light is also transmitted through the diamond to illuminate the sample to be tested. The fluorescence generated by the diamond and the excitation light from the sample are collected by the objective lens and transmitted to the first dichroic film. The first dichroic film guides the fluorescence to the second dichroic film, and the second dichroic film guides the excitation light to the illumination imaging module for imaging. The microwave module is used to radiate microwaves onto the diamond.

2. The diamond NV color center wide-field detection system with multiplexed light source according to claim 1, characterized in that: The first dichroic filter is an interference-type long-pass or short-pass dichroic filter with a cutoff wavelength λ1 of 532nm < λ1 < 610nm, and the first dichroic filter satisfies: It ranges from 0.5% to 10%, of which, The first dichroic filter directs the excitation light to the second dichroic filter, The incident light power is the excitation light transmitted from the objective lens to the first dichroic plate.

3. The diamond NV color center wide-field detection system with multiplexed light source according to claim 1, characterized in that: The second dichroic filter is an interference-type long-pass or short-pass dichroic filter with a cutoff wavelength λ2 of 532nm < λ2 < 637nm, and the second dichroic filter satisfies the following: It is 0-0.5%, of which, To excite the light to be directed to the wide-field imaging module, The light power that directs the excitation light from the first dichroic filter to the second dichroic filter.

4. The diamond NV color center wide-field detection system with multiplexed light source according to claim 1, characterized in that: The excitation light module includes a laser and a first condenser lens. The first condenser lens is used to converge and shape the laser beam emitted from the excitation light to obtain a beam size that meets the requirements of the objective lens.

5. The diamond NV color center wide-field detection system with multiplexed light source according to claim 4, characterized in that: The excitation light module also includes frosted glass, located between the first condenser lens and the first dichroic filter.

6. The diamond NV color center wide-field detection system with multiplexed light source according to claim 1, characterized in that: The excitation light module includes a light-emitting diode and one or more focusing lenses. The focusing lenses are used to converge and collimate the light emitted from the light-emitting diode to reduce the divergence angle of the light.

7. The diamond NV color center wide-field detection system with multiplexed light source according to claim 1, characterized in that: The wide-field imaging module includes a first filter, a first imaging lens, and a first imaging camera arranged sequentially along the light collection direction; the illumination imaging module includes a second imaging lens and a second imaging camera arranged sequentially along the light collection direction.

8. The diamond NV color center wide-field detection system with multiplexed light source according to claim 1, characterized in that: It also includes a bias magnetic field module for applying a bias magnetic field to the probe.

9. The diamond NV color center wide-field detection system with light source multiplexing according to any one of claims 1-8, characterized in that: It also includes a moving device, on which the sample to be tested is placed on a platform and the sample to be tested can be moved; and a data processing module, connected to the wide-field imaging module and the illumination imaging module, for reading fluorescence imaging data and illumination imaging images respectively and processing and analyzing them, and connected to the microwave module for controlling the microwave frequency radiated by it.

10. A method for illumination detection in synchronous wide-field imaging, characterized in that, A wide-field detection system for diamond NV color centers using sustainable illumination as described in any one of claims 1-9, the method comprising: placing the sample to be tested below the diamond, illuminating it with the excitation light from the excitation light module, imaging the fluorescence with the wide-field imaging module, and imaging the excitation light for illumination with the illumination imaging module; when measuring the magnetic field of the sample to be tested, performing ODMR measurement and reading the fluorescence imaging data output by the wide-field imaging module, and obtaining the magnetic field intensity distribution based on this data; when it is necessary to observe the sample to be tested, reading the illumination imaging image output by the illumination imaging module, which is the imaging image of the sample surface.

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