Primer pair for detecting combined lactobacillus salivarius, kit, detection method and application

By designing primer pairs and kits for saliva-associated lactobacillus, and combining PCR amplification and gel electrophoresis, the problems of low efficiency and high cost of traditional detection methods have been solved, achieving rapid and accurate detection of saliva-associated lactobacillus, which is suitable for the detection of fermented dairy products and fermented beverages.

CN121472436APending Publication Date: 2026-02-06CHANGSHU INSTITUTE OF TECHNOLOGY
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Patent Information

Application Number
CN202511807030.8
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-12-03
Publication Date
2026-02-06

AI Technical Summary

Technical Problem

Existing technologies for detecting Lactobacillus salivarius are inefficient and costly, making it difficult to quickly and accurately identify whether Lactobacillus salivarius has been added to fermented dairy products and fermented beverages.

Method used

This invention provides a primer pair (dnaA-L and dnaA-R) for detecting Lactobacillus salivarius, along with corresponding kits and detection methods, including PCR amplification and gel electrophoresis steps, enabling rapid and accurate detection of the presence of Lactobacillus salivarius.

Benefits of technology

Rapid detection of Lactobacillus in saliva was achieved with a sensitivity of 4.41 pg/PCR, and the detection time was shortened to 24 hours, which significantly improved detection efficiency and reduced costs.

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Abstract

The invention discloses a primer pair for detecting combined lactobacillus salivarius, a kit, a detection method and application, and belongs to the technical field of microbiological detection. In order to solve the problems of low detection efficiency and high cost of the traditional detection method of the combined lactobacillus salivarius in the prior art, the invention provides the primer pair, the kit and the detection method for detecting the combined lactobacillus salivarius, the primer pair and the kit can specifically judge whether the combined lactobacillus salivarius is the combined lactobacillus salivarius, and the detection result is accurate and reliable; the detection method is simple and easy to implement, the fastest detection time of the combined lactobacillus salivarius is only 24 hours, the detection efficiency is improved, and meanwhile, the detection cost is reduced. The primer pair, the kit and the detection method provided by the invention have great application value for judging whether the fermented dairy product or the fermented beverage product is added with the saliva combined lactobacillus or not.
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