Application of dental pulp stem cell exosome in preparation of medicine for treating and preventing osteoarthritis

By preparing dental pulp stem cell exosomes and applying them to drugs, the shortcomings of existing osteoarthritis treatments have been addressed, achieving the protection and repair of chondrocytes and demonstrating the important significance of dental pulp stem cell exosomes in the treatment of osteoarthritis.

CN121534086APending Publication Date: 2026-02-17HEBEI MEDICAL UNIVERSITY
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Patent Information

Application Number
CN202511910585.5
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-12-17
Publication Date
2026-02-17

AI Technical Summary

Technical Problem

Existing treatments for osteoarthritis cannot fundamentally repair cartilage, regulate the intra-articular environment, or stop disease progression. Furthermore, direct stem cell transplantation presents challenges such as complex procedures, poor safety profiles, and immune rejection.

Method used

By utilizing dental pulp stem cell exosomes, a primary articular chondrocyte system was constructed in vitro to prepare dental pulp stem cell exosomes and apply them to drugs. The bioactive substances secreted by these exosomes, such as cytokines, growth factors, and miRNAs, can inhibit endoplasmic reticulum stress-induced senescence of chondrocytes and protect and repair cartilage.

Benefits of technology

Dental pulp stem cell exosomes effectively inhibited IL-1β-promoted endoplasmic reticulum stress-induced senescence in chondrocytes, demonstrating strong cartilage protection and repair capabilities. They have the advantages of simple operation, high safety, and avoidance of immune rejection, and can improve the pathological process of osteoarthritis.

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Abstract

The invention relates to the technical field of biological medicine, and provides application of a dental pulp stem cell exosome in preparation of a medicine for treating and preventing osteoarthritis, a preparation method of the dental pulp stem cell exosome comprises the following steps: S1, obtaining dental pulp tissue from a dental pulp cavity of an in-vitro tooth; s2, pretreating dental pulp tissues, adding digestive juice, digesting, centrifuging, discarding supernatant, adding a complete culture medium containing antibiotics into precipitates, mixing, filtering and centrifuging to obtain dental pulp stem cells; and S3, culturing the dental pulp stem cells by using a complete culture medium, when the confluence degree of the dental pulp stem cells reaches 60-80%, replacing the serum-free culture medium for continuous culture, collecting the dental pulp stem cell conditioned culture medium, and performing differential centrifugation to obtain the dental pulp stem cell exosome. According to the technical scheme, the regulating effect of the dental pulp stem cell exosome on endoplasmic reticulum stress and the mechanism of the dental pulp stem cell exosome for improving cartilage cell degeneration are explored.
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