Kit for identifying five target tapeworms by using specific primers and application of kit
By combining specific primers and RAA isothermal nucleic acid amplification technology with colloidal gold immunochromatography, a rapid and simplified detection of Echinococcus granulosus, Echinococcus multilocularis, Taenia vesicularis, Taenia multicephalus, and Diploporium canis was achieved. This solves the problems of complex and time-consuming detection in existing technologies and is suitable for field applications at the grassroots level.
Patent Information
- Application Number
- CN202610108829.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2026-01-27
- Publication Date
- 2026-05-12
AI Technical Summary
Existing detection methods for dogs infected with Echinococcus granulosus, Taenia lentigines, Taenia multicephala, and Diploporium canis are complex, time-consuming, and require sophisticated equipment, making it difficult to meet the needs for rapid and visual on-site detection.
By employing specific primers combined with RAA isothermal nucleic acid amplification technology and colloidal gold immunochromatography, specific primers targeting five tapeworm species were designed. The results were directly interpreted by the naked eye using colloidal gold immunochromatographic nucleic acid test strips after amplification at 37℃, simplifying the testing process and making it suitable for field use at the grassroots level.
It enables simultaneous and rapid detection of five target tapeworms, reducing detection complexity and instrument requirements, improving detection accuracy and efficiency, making it suitable for field applications at the grassroots level, and enabling detection to be completed within 5-20 minutes and results to be interpreted visually.