A method for preparing a Chinese herbal medicine nano-silver antibacterial colloidal solution by laser physics

Abstract

This invention discloses a method for preparing a nano-silver antibacterial colloidal solution from traditional Chinese medicine using a laser physical method. First, an initial nano-silver colloidal solution is obtained in 20 ml of a PVP aqueous solution (50 ppm) using a liquid-phase laser ablation method. Specific parameters are: laser wavelength 1064 nm, frequency 25 Hz, and time 10 min. Then, 1 ml of honeysuckle extract, 25 ml of honeysuckle hydrosol, 25 ml of centella asiatica hydrosol, and 80 ml of water are added. Laser irradiation continues under stirring, with specific parameters: laser wavelength 532 nm, frequency 10 Hz, and time 3 min. After 1-3 days, the color changes from pale yellow to golden yellow / golden orange, yielding the final nano-silver antibacterial colloidal solution from traditional Chinese medicine. This method provides a new approach and idea for the green development and environmentally friendly production of safe and effective antibacterial and antiviral products; it is universally applicable and can be extended to other specific application types of nanoparticles and traditional Chinese medicine ingredients.

Description

Technical Field

[0001] This invention relates to the preparation technology of antibacterial colloids containing traditional Chinese medicine components, and more particularly to a method for preparing a traditional Chinese medicine nano-silver antibacterial colloid solution by laser physical method. Background Technology

[0002] Nanosilver has attracted widespread attention and research due to its broad-spectrum and potent antibacterial and antiviral effects. However, its potential cytotoxicity and metabolic issues have also led to considerable controversy surrounding its widespread application. How to effectively and safely utilize the antibacterial and antiviral effects of nanosilver has become a major research topic.

[0003] The applicant discovered that the antibacterial and antiviral effects of nano-silver are closely related to its size; the smaller the size, the stronger the effect, and the lower the concentration required. When the size is between 5-20 nm, a concentration of 10 ppm can achieve a 99.9% bactericidal effect. The applicant is also considering combining nano-silver with traditional Chinese medicine antibacterial ingredients to further enhance its antibacterial effect while improving its biocompatibility.

[0004] Current preparation methods are all concentrated in chemical methods, either obtaining nano-silver through chemical methods first and then compounding it with traditional Chinese medicine ingredients, or using traditional Chinese medicine ingredients as reducing agents to reduce silver nitrate solution. In these processes, other impurity ions inevitably remain, or temperature conditions exceeding 100 degrees Celsius are involved.

[0005] Obtaining a low-concentration, herbal nano-silver antibacterial colloidal solution with no impurities and a size of less than 5nm under normal temperature and pressure is an essential step in developing safe and efficient antibacterial and disinfectant products, but it is also a hurdle in existing technologies.

[0006] In view of this, the present invention is hereby proposed. Summary of the Invention

[0007] The purpose of this invention is to provide a method for preparing a nano-silver antibacterial colloidal solution of traditional Chinese medicine using a laser physical method, so as to solve the above-mentioned technical problems existing in the prior art.

[0008] The objective of this invention is achieved through the following technical solution:

[0009] A method for preparing antibacterial colloidal solutions of traditional Chinese medicine nano-silver using laser physics includes:

[0010] Step 1: The silver target is etched in PVP aqueous solution using liquid phase laser ablation to obtain the initial nano-silver colloidal solution.

[0011] Step 2: Add honeysuckle extract, honeysuckle hydrosol, centella asiatica hydrosol and water, and continue laser irradiation while stirring;

[0012] Step 3: After laser irradiation, let it stand for a period of time to obtain the final herbal nano-silver antibacterial colloidal solution.

[0013] Compared with existing technologies, the method for preparing antibacterial colloidal solutions of traditional Chinese medicine nano-silver using laser physics provided by this invention is the first to utilize traditional Chinese medicine components as protective agents, grafting them onto the surface of nano-silver particles obtained by laser physics. Further laser irradiation reduces the size of the nano-silver, comprehensively improving the utilization rate of nano-silver and the antibacterial biological effects of traditional Chinese medicine. This method requires no addition of any other reducing agents, stabilizers, or other chemical reagents, has no requirements on the amount of raw materials, is user-friendly, simple to operate, green, efficient, and suitable for large-scale production. Moreover, the obtained nano-silver particles are small (<3 nm) and uniformly distributed. This design fully combines the antibacterial activity of biological raw materials and nanomaterials. All components are used in very small amounts in the final formulation, yet the effect is significant. This method provides a new approach and idea for the green development and environmentally friendly production of safe antibacterial and antiviral products. Furthermore, this method is universal and can be extended to other specific application types of nanoparticles and traditional Chinese medicine ingredients. The following section will describe the method in detail using the preparation of nano-silver and the combination of effective components from honeysuckle and centella asiatica as examples. This combination involves the following raw materials and their functions: nano silver - antibacterial and disinfectant; PVP - antibacterial sustained release, biocompatible; honeysuckle extract - effective biological antibacterial ingredient; honeysuckle hydrosol - soothing and repairing; centella asiatica hydrosol - repairing and stabilizing. Attached Figure Description

[0014] Figure 1 A schematic flowchart of a method for preparing a nano-silver antibacterial colloidal solution of traditional Chinese medicine using a laser physical method is provided for embodiments of the present invention;

[0015] Figure 2 This is a photograph of the color change of the herbal nano-silver colloidal solution after irradiation in an embodiment of the present invention.

[0016] Figure 3 The figures show the TEM morphology, high-resolution, and energy dispersive spectroscopy of the products in the embodiments of the present invention; in the figures: (a) is the sample obtained after the initial nano-silver colloidal solution has undergone the same irradiation process; (b) is the nano-silver colloidal solution sample under the honeysuckle and centella asiatica formulation;

[0017] Figure 4 These are colony images and bactericidal rate diagrams of different samples after being treated with Escherichia coli and Staphylococcus aureus in embodiments of the present invention. Detailed Implementation

[0018] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of the present invention, and not all of them, and do not constitute a limitation on the present invention. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are within the protection scope of the present invention.

[0019] First, the following explanations are provided for the terms that may be used in this article:

[0020] The terms “including,” “contains,” “comprising,” “having,” or other similar semantic descriptions shall be interpreted as non-exclusive inclusion.

[0021] The term "composed of" excludes any technical features not expressly listed. When used in a claim, it closes the claim to exclude all technical features other than those expressly listed, except for associated conventional impurities. If the term appears only in a clause of a claim, it limits the claim to the elements expressly listed in that clause; elements recited in other clauses are not excluded from the overall claim.

[0022] The technical solution provided by this invention will be described in detail below. Contents not described in detail in the embodiments of this invention are prior art known to those skilled in the art. Where specific conditions are not specified in the embodiments of this invention, they shall be performed according to conventional conditions in the art or conditions recommended by the manufacturer. Reagents or instruments used in the embodiments of this invention whose manufacturers are not specified are all conventional products that can be purchased commercially.

[0023] like Figure 1 As shown, a method for preparing a nano-silver antibacterial colloidal solution of traditional Chinese medicine using laser physics includes:

[0024] Step 1: The silver target is etched in PVP aqueous solution using liquid phase laser ablation to obtain the initial nano-silver colloidal solution.

[0025] Step 2: Add honeysuckle extract, honeysuckle hydrosol, centella asiatica hydrosol and water, and continue laser irradiation while stirring;

[0026] Step 3: After laser irradiation, let it stand for a period of time to obtain the final herbal nano-silver antibacterial colloidal solution.

[0027] In step 1, liquid phase laser ablation is performed in 20 volumes of 50 ppm PVP aqueous solution.

[0028] In step 1, the specific parameters of the liquid phase laser ablation method are: laser wavelength 1064nm, frequency 25HZ, and time 10 min.

[0029] In step 2, add 1 volume of honeysuckle extract, 25 volume of honeysuckle hydrosol, 25 volume of centella asiatica hydrosol, and 80 volume of water.

[0030] In step 2, the parameters for laser irradiation are: laser wavelength 532 nm, frequency 10 Hz, and time 3 min.

[0031] In step 3, after laser irradiation, the solution is left to stand for 1-3 days. The color changes from light yellow to golden yellow / golden orange, and the final herbal nano-silver antibacterial colloidal solution can be obtained.

[0032] In summary, the method for preparing antibacterial colloidal solutions of traditional Chinese medicine nanoparticles using laser physics in this invention leverages the advantages of liquid-phase laser technology in preparing nanocolloid solutions: small size, charged structure, stable dispersion, ease of grafting and compounding, and abundance of defects. This allows for the effective grafting of traditional Chinese medicine components with unique biological effects, achieving a synergistic improvement in the performance of the nanocolloid solution. Furthermore, because the components of traditional Chinese medicine are complex and diverse, the laser acts as a protective agent, further inhibiting the growth of silver nanoparticles and maintaining their small size. This directly yields a highly uniformly dispersed nanocolloid solution with a small size (<3 nm). This effective combination further reduces the amount of silver nanoparticles used in practical applications, improving their efficiency and biosafety. Since the raw materials are only active elements (Ag), sustained-release PVP, and traditional Chinese medicine, there are no other sources of impurities throughout the experimental process. The resulting final product is highly efficient and pure, with no other chemical residues. Moreover, due to the top-down preparation advantage of the laser physics method, the entire synthesis process is green and environmentally friendly. This invention expands the advantages of laser physics in combining biological raw materials and nanomaterials, provides new safety strategies for the application of nanomaterials in actual production and daily life, and offers new ways for the green development and environmentally friendly production of highly safe antibacterial and antiviral products.

[0033] Since the existing technology is still mainly based on chemical methods, or using honeysuckle extract as a reducing agent, or combining honeysuckle after preparing nano-silver, regardless of the method, preparing nanoparticles smaller than 3nm is a hurdle. Using honeysuckle extract as a reducing agent, the nanoparticles are basically larger than 10nm, while ensuring that they are below 3nm is complicated, leaves a lot of chemical residues, and has poor safety.

[0034] The key feature of this application is its purely physical method, which leaves no chemical residue. Traditional Chinese medicine not only enhances the antibacterial effect of nano-silver but also protects its size. Furthermore, this approach not only uses honeysuckle but also incorporates centella asiatica, considering the sensitizing effect of nano-silver. The holistic use of traditional Chinese medicine ingredients acts as a protective agent, while simultaneously enhancing the antibacterial and anti-inflammatory effects.

[0035] To more clearly demonstrate the technical solution and its effects provided by the present invention, the method for preparing nano-silver antibacterial colloidal solution of traditional Chinese medicine by laser physical method provided by the present invention will be described in detail below with specific embodiments.

[0036] Example 1

[0037] like Figures 1 to 4 As shown:

[0038] First, an initial colloidal solution of nano-silver was obtained in 20 ml of PVP aqueous solution (50 ppm) using liquid-phase laser ablation. The specific parameters were: laser wavelength 1064 nm, frequency 25 Hz, and time 10 min. Then, 1 ml of honeysuckle extract, 25 ml of honeysuckle hydrosol, 25 ml of centella asiatica hydrosol, and 80 ml of water were added. Laser irradiation was continued with stirring, using the parameters: laser wavelength 532 nm, frequency 10 Hz, and time 3 min. After 1-3 days, the color changed from yellow to golden orange. Figure 2 This process yields the final herbal nano-silver antibacterial colloidal solution.

[0039] To characterize the morphology of the product, we performed TEM morphology, high-resolution imaging, and energy dispersive spectroscopy (EDS). Figure 3 (a) is a sample obtained after the initial silver nanoparticle colloidal solution under the same irradiation process. The energy spectrum shows that the particles are silver particles, and the TEM image shows that the particle size is between 5-20 nm. The high-resolution image in the upper right corner shows that the particles are crystalline. (b) is a sample of silver nanoparticle colloidal solution with honeysuckle and centella asiatica. The crystalline silver particles can still be clearly distinguished from the energy spectrum and high-resolution image. The TEM image shows that under laser irradiation, after the encapsulation of the herbal components, smaller and more uniform silver nanoparticles can be obtained, with an average size of about 1-3 nm. Such a specific size and the encapsulation state of the herbal components will exhibit special properties in the biological field.

[0040] To further determine the biological properties of the product, we evaluated the antibacterial activity of the corresponding materials (Table 1). Figure 4The samples taken were: blank control, honeysuckle extract (honeysuckle's intrinsic components are recognized to have antibacterial effects), honeysuckle hydrosol, herbal formula 1 (example component: nano-silver concentration of 9.8 ppm), herbal formula 2 (example component: nano-silver concentration of 5 ppm), herbal formula 3 (example component: nano-silver concentration of 3.4 ppm), and nano-silver (concentration of 10 ppm). Table 1 shows the OD600 values ​​of the growth changes of different samples after treatment with E. coli. From the table, we can observe that all herbal formulas still showed antibacterial effects after 6 hours, while E. coli in other samples had begun to recover after 6 hours.

[0041]

[0042] Table 1. Changes in OD600 values ​​of Escherichia coli growth after treatment with different samples.

[0043] Figure 4 The images show colony images and bactericidal rates of *Escherichia coli* and *Staphylococcus aureus* in different samples. From the figures, we can see that honeysuckle extract, due to its high content of active antibacterial biological components, exhibits bactericidal rates of 97.5% and 99.9%, respectively. Honeysuckle hydrosol, however, has fewer active antibacterial components but more nutrients, resulting in significant bacterial proliferation. Notably, the herbal formula, with a nano-silver concentration of 3.4 ppm, still shows a better bactericidal rate than 10 ppm nano-silver. These results fully demonstrate the antibacterial and bactericidal biological advantages of the herbal nano-silver composite system.

[0044] This invention utilizes traditional Chinese medicine components as protective agents, first grafting them onto the surface of silver nanoparticles obtained through laser physics, and then further reducing the size of the silver nanoparticles under laser irradiation, thereby comprehensively improving the utilization rate of silver nanoparticles and the antibacterial biological effects of traditional Chinese medicine. No other reducing agents, stabilizers, or other chemical reagents are required, the entire preparation process is residue-free, simple to operate, green, efficient, and suitable for mass production. Moreover, the obtained silver nanoparticles are small (<3 nm) and uniformly distributed. This design fully combines the antibacterial activity of biological raw materials and nanomaterials. All components are used in very small amounts in the final formulation, yet the effect is significant. This method provides a new approach and idea for the green development and environmentally friendly production of safe antibacterial and antiviral products. Furthermore, this method is universal and can be extended to other specific application types of nanoparticles and traditional Chinese medicine components.

[0045] The preparation of nano-silver was chosen in combination with the effective components of honeysuckle and centella asiatica. The raw materials and their functions are as follows: nano-silver - antibacterial and disinfectant; PVP - antibacterial sustained release and biocompatibility; honeysuckle extract - effective biological antibacterial component; honeysuckle hydrosol - soothing and repairing; centella asiatica hydrosol - repairing and stabilizing.

[0046] The above description is merely a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the scope of the technology disclosed in the present invention should be included within the scope of protection of the present invention. Therefore, the scope of protection of the present invention should be determined by the scope of the claims. The information disclosed in the background section is intended only to enhance the understanding of the overall background technology of the present invention and should not be construed as an admission or implication in any way that such information constitutes prior art known to those skilled in the art.

Claims

1. A method for preparing a Chinese herbal medicine nanosilver antibacterial colloidal solution by laser physics, characterized in that, include: Step 1: Ag target material is etched in PVP aqueous solution using liquid phase laser ablation to obtain initial nano-silver colloidal solution; Step 2: Add honeysuckle extract, honeysuckle hydrosol, centella asiatica hydrosol and water, and continue laser irradiation while stirring; Step 3: After laser irradiation, let it stand for a period of time to obtain the final herbal nano-silver antibacterial colloidal solution.

2. The method for preparing Chinese herbal medicine nano-silver antibacterial colloidal solution by laser physical method according to claim 1, characterized in that, In step 1, liquid phase laser ablation is performed in 20 volumes of 50 ppm PVP aqueous solution.

3. The method for preparing Chinese herbal medicine nano-silver antibacterial colloidal solution by laser physical method according to claim 2, characterized in that, In step 1, the specific parameters of the liquid phase laser ablation method are: laser wavelength 1064nm, frequency 25HZ, and time 10min.

4. The method for preparing a nano-silver antibacterial colloidal solution of traditional Chinese medicine using laser physical method according to claim 1, characterized in that, In step 2, add 1 volume of honeysuckle extract, 25 volume of honeysuckle hydrosol, 25 volume of centella asiatica hydrosol, and 80 volume of water.

5. The method for preparing a nano-silver antibacterial colloidal solution of traditional Chinese medicine using laser physical method according to claim 4, characterized in that, In step 2, the parameters for laser irradiation are: laser wavelength 532 nm, frequency 10 Hz, and time 3 min.

6. The method for preparing a nano-silver antibacterial colloidal solution of traditional Chinese medicine using a laser physical method according to any one of claims 1 to 5, characterized in that, In step 3, after laser irradiation, the solution is left to stand for 1-3 days. The color changes from light yellow to golden yellow / golden orange, and the final herbal nano-silver antibacterial colloidal solution can be obtained.

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