A process for the preparation of tenuazonic acid

By optimizing the culture medium and using three-stage chromatographic purification technology, the problems of low efficiency and high cost in the preparation of TeA in existing technologies have been solved, and high-yield and high-purity TeA preparation has been achieved, which is suitable for the detection and toxicological studies of TeA in food and feed.

CN122648508APending Publication Date: 2026-08-28SHANGHAI ACAD OF AGRI SCI
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Patent Information

Application Number
CN202610996124.2
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-07-06
Publication Date
2026-08-28

AI Technical Summary

Technical Problem

Existing technologies are difficult to prepare high-purity TeA from Alternaria alternifolia in an efficient and economical manner. Furthermore, the preparation methods are cumbersome, time-consuming, and costly, which cannot meet the needs of TeA detection and toxicological studies in food and feed.

Method used

Alternaria alternata was cultured in an optimized potato-glucose-agar/water medium. Combined with acetonitrile extraction, n-butanol extraction, macroporous resin decolorization, and three-stage chromatographic purification (reversed-phase preparation-gel size exclusion-normal-phase preparation) techniques, TeA was efficiently separated and purified.

Benefits of technology

It achieves high-yield (1003.1 mg/kg) and high-purity (>99%) preparation of TeA, simplifies the operation process, reduces production costs, and is suitable for the detection and toxicological studies of TeA.

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Abstract

The application discloses a method for preparing fine fusaric acid, which comprises the following steps: toxin production culture, sample extraction, preliminary purification, reverse-phase preparation, gel chromatography separation and normal-phase preparation. The method for preparing fine fusaric acid provided by the application improves material steady state and preparation efficiency, greatly reduces production cost, and has a purity of more than 99%, and can be used as a standard product for fine fusaric acid detection, prevention and control and toxicology research in food and feed.
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