Microscope objective automatic recognition system and method

Abstract

The present application relates to the field of microscope optical detection and intelligent identification technology, and particularly relates to a microscope objective automatic identification system and method, the system comprising: a microscope objective, a color ring encoding disc is arranged on the back end surface of the microscope objective; a coaxial optical fiber, one end of the coaxial optical fiber is directed to the color ring encoding disc, and the other end of the coaxial optical fiber is connected to a color identification sensor, the coaxial optical fiber is used for transmitting illumination light to the color ring encoding disc of the microscope objective, and acquiring a reflected light signal carrying color information; the color identification sensor is used for acquiring the reflected light signal and converting the reflected light signal into RGB color data; a master control unit is used for receiving the RGB color data, and decoding and identifying parameter information of the microscope objective according to a pre-stored color ring encoding and objective parameter mapping table. The present application solves the problems of high cost, single identification dimension, poor generalization of modification and short service life of the identification mode of the microscope objective in the prior art.

Description

Technical Field

[0001] This invention belongs to the field of microscope optical inspection and intelligent recognition technology, and particularly relates to an automatic recognition method and system for microscope objectives. Background Technology

[0002] Automatic identification technology for microscope objectives is a crucial foundation for achieving intelligent and automated imaging in microscopes. Currently, existing automatic identification methods for microscope objectives mainly include absolute coding turntable identification, Hall effect magnetic sensing identification, step pulse counting identification, RFID tag identification, and turntable color block identification.

[0003] However, all of the aforementioned existing technologies have varying degrees of drawbacks. Encoded turntable recognition, magnetic sensing recognition, and grating recognition schemes are costly, require significant modifications to the original microscope structure, and are incompatible with common objectives. RFID recognition schemes require the installation of electronic tags inside the objective lens, involving complex assembly processes, and these tags are susceptible to damage during routine microscope maintenance due to their susceptibility to high temperatures and organic solvents. Traditional color recognition schemes involve attaching monochrome color blocks to the surface of the objective turntable. This method only identifies the turntable position and not the objective lens itself; furthermore, the color blocks are exposed to the external environment and are easily faded by dust, oil, and alcohol wiping, resulting in poor recognition stability.

[0004] In summary, existing technologies generally suffer from the following technical defects: they cannot achieve multi-dimensional coding recognition based on the objective lens itself; they cannot distinguish between large batches of objectives with different magnifications, numerical apertures (NA), parfocal distances, and batch models using low-cost structures; they have a single recognition dimension; they have weak anti-interference capabilities; they have poor versatility for modification; and they have a short service life. Summary of the Invention

[0005] In view of this, the present invention aims to provide an automatic identification method and system for microscope objectives, so as to solve the problems of high cost, single identification dimension, poor versatility and short service life of the existing microscope objective identification methods.

[0006] To achieve the above objectives, the technical solution created by this invention is implemented as follows: In a first aspect, the present invention provides an automatic microscope objective lens recognition system, comprising: The microscope objective lens has a color ring coding disk on its rear end face; A coaxial optical fiber, one end of which points to the color ring encoding disk and the other end of which is connected to the color recognition sensor, is used to transmit illumination light to the color ring encoding disk of the microscope objective and to acquire reflected light signals carrying color information. A color recognition sensor is used to acquire the reflected light signal and convert it into RGB color data; The main control unit is used to receive the RGB color data and decode and identify the parameter information of the microscope objective according to the pre-stored color wheel encoding and objective parameter mapping table.

[0007] Furthermore, the coaxial optical fiber integrates an illumination optical fiber and a color receiving optical fiber. The illumination optical fiber is connected to the light source output port of the color recognition sensor, and the color receiving optical fiber is connected to the light signal input port of the same color recognition sensor.

[0008] Furthermore, the color ring encoding disk includes N color rings, and the N color rings are arranged concentrically in a radial direction, with N taking the value of 2, 3 or 4.

[0009] Furthermore, there are N coaxial optical fibers, each corresponding to one of the N color rings, with one end of each of the N coaxial optical fibers pointing to its corresponding color ring.

[0010] Furthermore, there are N color recognition sensors, each connected to one of the N coaxial optical fibers.

[0011] Furthermore, the color ring code and objective lens parameter mapping table records the objective lens parameter information corresponding to each color ring code disk. The objective lens parameter information includes at least one of the following: objective lens magnification, numerical aperture (NA), objective lens type, and production batch.

[0012] Secondly, the present invention provides a microscope objective lens for use in the microscope objective lens automatic identification system provided by the present invention. The rear end face of the microscope objective lens is provided with a color ring encoding disk, which is used to characterize the parameter information of the microscope objective lens.

[0013] Thirdly, this invention provides an automatic identification method for microscope objectives, comprising the following steps: S10. Stable illumination light is output to the rear end face of the microscope objective through an illumination optical fiber. The rear end face of the microscope objective is provided with a color ring encoding disk, which includes N color rings. S20. Receive the reflected light signal carrying color information after being reflected by the color ring encoding disk through the color receiving optical fiber; S30. Acquire the reflected light signal through a color recognition sensor and convert it into RGB color data; S40. The main control unit receives the RGB color data and decodes and identifies the parameter information of the microscope objective according to the pre-stored color wheel encoding and objective parameter mapping table.

[0014] Furthermore, in step S10, there are N lighting optical fibers, and the illumination output from the N lighting optical fibers respectively illuminates the N color rings perpendicularly.

[0015] Furthermore, after step S40, the method further includes: S50. Based on the parameter information of the microscope objective, automatically adapt and adjust at least one of the microscope's scale graduations, exposure parameters, light source brightness, and focusing parameters. Compared with the prior art, the present invention can achieve the following beneficial effects: (1) This invention is the first to combine coaxial optical fiber with objective lens color coding to achieve isolated transmission of reflected light signals, so that the sensor can be kept away from the oil, water vapor, dust and alcohol wiping environment inside the microscope. It has strong anti-interference ability and completely solves the problem of recognition failure caused by the exposure of the sensor in traditional color recognition schemes.

[0016] (2) The present invention creates a color ring coding disk on the microscope objective lens body to directly encode the objective lens body, which can identify the real parameters of the objective lens rather than just the position of the turntable, fundamentally solving the defect of the traditional solution that cannot obtain the objective lens body information.

[0017] (3) The invention uses a color ring encoding disk composed of multiple concentric color rings, which can encode dozens or even hundreds of types, covering the entire series of microscope objective lens models, and meeting the identification needs of multiple objective lens models used in scientific research, industry and high-end testing scenarios.

[0018] (4) The invention uses a pure optical non-contact recognition method to realize the automatic recognition of microscope objectives. There are no mechanical contacts or wear parts. It recognizes the objectives as soon as it is powered on. There is no need for origin calibration, pulse counting, or risk of losing synchronization. This significantly reduces the maintenance cost and operation complexity of the system.

[0019] (5) The hardware cost created by this invention is extremely low, which is significantly lower than that of absolute coding turntable, magnetic grating, RFID and other solutions. Moreover, there is no need to replace the objective turntable or modify the lens structure. Ordinary objective lenses can be directly upgraded with color rings to achieve intelligent upgrades, and the adaptability is extremely strong.

[0020] (6) The color ring created by this invention can be made by using a scratch-resistant UV coating or a ceramic high-temperature coloring process, and has the characteristics of being wear-resistant, corrosion-resistant, and colorfast, with a long service life.

[0021] (7) The invention creates a system that can directly identify the parameters of the objective lens itself and achieve fully automatic matching of microscope imaging parameters, raising the level of intelligence of the traditional solution, which can only identify the turntable station but cannot identify the real parameters of the objective lens, to a whole new level. Attached Figure Description

[0022] The accompanying drawings, which form part of this invention, are used to provide a further understanding of the invention. The illustrative embodiments and descriptions of the invention are used to explain the invention and do not constitute an undue limitation of the invention. In the drawings: Figure 1A schematic diagram of the structure of the automatic microscope objective recognition system described in the embodiment of the present invention; Figure 2 A schematic diagram of the structure of the microscope objective lens described in the embodiment of the present invention; Figure 3 This is a schematic flowchart of the automatic identification method for microscope objectives described in an embodiment of the present invention.

[0023] Explanation of reference numerals in the attached figures: 10. Microscope objective lens; 11. Color ring encoder disk; 111. First color ring; 112. Second color ring; 113. Third color ring; 20. Coaxial optical fiber; 21. Illumination optical fiber; 22. Color receiving optical fiber; 30. Color recognition sensor; 40. Main control unit; 41. Communication line. Detailed Implementation

[0024] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to the accompanying drawings and specific embodiments. It should be understood that the specific embodiments described herein are only for explaining the invention and do not constitute a limitation thereof. Similar elements in different embodiments are referred to by associated similar element reference numerals. In the following embodiments, many details are described to facilitate a better understanding of the invention. However, those skilled in the art will readily recognize that some features may be omitted in different situations, or may be replaced by other elements, materials, or methods. In some cases, some operations related to the invention are not shown or described in the specification. This is to avoid obscuring the core parts of the invention with excessive description. For those skilled in the art, detailed description of these related operations is not necessary; they can fully understand the related operations based on the description in the specification and general technical knowledge in the art.

[0025] It should be noted that, unless otherwise specified, the embodiments and features described in this invention can be combined to form various implementations. Furthermore, the order of the steps or actions in the method description can be changed or adjusted in a manner readily apparent to those skilled in the art. Therefore, the various orders in the specification and drawings are merely for the clear description of a particular embodiment and do not imply a mandatory order, unless otherwise stated that a particular order must be followed.

[0026] In the description of this invention, it should be understood that the terms "center," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," and "counterclockwise," etc., indicating orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation on this invention. The term "based on" should be understood as "at least partially based on." Furthermore, the terms "first," "second," etc., are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Thus, features defined with "first," "second," etc., may explicitly or implicitly include one or more of that feature. In the description of this invention, unless otherwise stated, "a plurality of" means two or more, and the term "including" means "including but not limited to." Various embodiments of the present invention may exist in the form of a range; it should be understood that the description in the form of a range is merely for convenience and brevity and should not be construed as a rigid limitation on the scope of the invention; therefore, it should be considered that the range description has specifically disclosed all possible sub-ranges and single numerical values ​​within that range; for example, it should be considered that the range description from 1 to 6 has specifically disclosed sub-ranges, such as from 1 to 3, from 1 to 4, from 1 to 5, from 2 to 4, from 2 to 6, from 3 to 6, etc., and single numbers within the range, such as 1, 2, 3, 4, 5, and 6, regardless of the range. Furthermore, whenever a numerical range is referred to herein, it means including any referenced number (fraction or integer) within the range referred to.

[0027] In the description of this invention, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art will understand the specific meaning of the above terms in this invention based on the specific circumstances.

[0028] The present invention will now be described in detail with reference to the accompanying drawings and embodiments.

[0029] Example 1 like Figure 1 , Figure 2As shown, the present invention provides an automatic microscope objective recognition system, including: a microscope objective 10, a coaxial optical fiber 20, a color recognition sensor 30, and a main control unit 40.

[0030] The microscope objective 10 has a color ring coding disk 11 on its rear end face (the end furthest from the sample). The color ring coding disk 11 has N color rings arranged concentrically in a radial direction, with each color ring's plane perpendicular to the central axis of the objective. The centers of all color rings are located on the central axis of the microscope objective 10, and N can be 2, 3, or 4. Each ring uses a single, fixed color, and each color ring is coated with a different color to form a specific color ring coding disk. Different color ring coding disks represent different models or parameters of microscope objectives. Through the combination coding of multiple color rings, a relatively small number of color rings can be used to encode and identify various objective information (e.g., 3 color rings, each with 8 colors to choose from, can create 512 possible codes), providing a large amount of information and strong scalability.

[0031] This invention does not limit the connection method between the microscope objective 10 and the color ring coding disk 11. In specific implementations, the color ring coding disk 11 can be directly bonded and fixed to the microscope objective 10 through an optically transparent adhesive layer. As another optional implementation, the color ring coding disk 11 is directly formed on the rear end face of the microscope objective 10 (e.g., the rear end cap of the microscope objective) by laser etching, forming an integrated structure with the microscope objective 10.

[0032] The number of coaxial optical fibers 20 and color recognition sensors 30 is the same as the number of color rings. More specifically, when the color ring encoder disk 11 has N color rings, there are also N coaxial optical fibers, each corresponding to one of the N color rings, with one end of each fiber pointing to its corresponding color ring. There are also N color recognition sensors, each connected to one of the N coaxial optical fibers. Thus, each color ring corresponds to one of the coaxial optical fibers, and the illumination fiber and color receiving fiber within each coaxial fiber form an independent detection channel with their respective color ring. These detection channels operate independently and in parallel, with each channel corresponding to one color sensor. With this configuration, the system can acquire the color data of all color rings at once without time-division scanning, offering advantages such as fast recognition speed, strong anti-crosstalk capability, and inter-channel fault isolation.

[0033] Please refer to the following at the same time Figure 2In this embodiment, the color ring encoding disk 11 has three color rings (N is 3), which are respectively designated as the first color ring 111, the second color ring 112, and the third color ring 113. The first color ring 111, the second color ring 112, and the third color ring 113 are arranged radially from the inside out. At this time, the number of coaxial optical fibers 20 and color recognition sensors 30 is also 3. Here, the three coaxial optical fibers 20 are respectively designated as the first coaxial optical fiber, the second coaxial optical fiber, and the third coaxial optical fiber, with the first coaxial optical fiber pointing towards the first color ring 111, the second coaxial optical fiber pointing towards the second color ring 112, and the third coaxial optical fiber pointing towards the third color ring 113. Each coaxial optical fiber 20 has its corresponding color recognition sensor 30.

[0034] The following example uses one coaxial fiber and its corresponding color recognition sensor 30. One end of the coaxial fiber 20 is connected to the color recognition sensor 30, and the other end points to the corresponding color ring in the color ring encoder disk 11. The coaxial fiber 20 integrates an illumination fiber 21 and a color receiving fiber 22. The illumination fiber 21 is connected to the light source output port of the color recognition sensor 30, and the color receiving fiber is connected to the light signal input port of the same color recognition sensor 30. The illumination fiber 21 can be a fiber bundle with an outer diameter of Φ0.5mm to Φ1.5mm, and the color receiving fiber 22 can be a fiber bundle with the same or slightly larger outer diameter as the illumination fiber 21. To eliminate ambient light interference, the outer periphery of the fiber bundle is covered with a light-shielding layer. This light-shielding layer is used to isolate stray ambient light inside the microscope cavity to ensure that the light signal transmitted inside the fiber is not interfered with by external light. In specific implementation, the end of the coaxial fiber 20 pointing to the color ring encoder disk 11 is fixed to the microscope body by a fixing bracket.

[0035] The plane of the color ring encoder disk 11 is parallel to the end face of the coaxial fiber 20 pointing to the color ring encoder disk 11. When the illumination light falls perpendicularly onto the color ring encoder disk 11 through the illumination fiber, it forms a millimeter-level light spot. The reflected light carrying color information transmits the signal back to the sensor through the color receiving fiber. At the same time, the concentric color ring structure of the color ring encoder disk 11 is not sensitive to the rotational deviation of the end of the coaxial fiber 20 pointing to the color ring encoder disk 11 around the objective lens axis, which effectively reduces the mechanical installation accuracy requirements.

[0036] The illumination light emitted by the color recognition sensor 30 is perpendicularly irradiated onto the surface of the color ring encoding disk 11 via the illumination fiber 21 in the coaxial fiber. The color rings on the color ring encoding disk 11 reflect the illumination light, forming a reflected light signal carrying the encoding information. This reflected light signal is transmitted back to the color recognition sensor 30 via the color receiving fiber 22 in the coaxial fiber.

[0037] More specifically, the LED light source built into the color recognition sensor 30 emits stable illumination light under the control of a constant current drive circuit. This illumination light is broadband white light, covering a spectral range of 380nm~780nm, encompassing the entire visible light band, to ensure effective excitation of the color characteristics of each color ring on the color ring encoding disk 11. The color coating on the surface of the color ring selectively absorbs and reflects light of different wavelengths, thereby forming a reflected light signal carrying color encoding information. The spectral composition of this reflected light signal corresponds one-to-one with the color of each color ring. The reflected light signal enters the color receiving fiber 22 at the end of the coaxial fiber 20. The color receiving fiber 22 isolates and transmits the reflected light signal along the fiber's interior to the color recognition sensor 30. During transmission, the reflected light signal is completely isolated from the external environment (including oil, water vapor, dust, and stray light inside the microscope), ensuring that the color information of the reflected light signal is not distorted or contaminated. The color recognition sensor 30 acquires the reflected light signal carrying color information and converts it into RGB color data for subsequent decoding and recognition by the main control unit 40. In an optional embodiment, the color sensor is a TCS34725 digital color sensor.

[0038] The main control unit (MCU) 40 is connected to N color recognition sensors 30 via communication lines 41, and reads the RGB color data output by each color recognition sensor in real time. The internal memory of the MCU 40 stores a color ring code and objective lens parameter mapping table. This table records the objective lens parameter information corresponding to each color ring color combination, including at least one of the following: objective lens magnification, numerical aperture (NA), objective lens type, and production batch. Under the unified control of the MCU 40, each color recognition sensor 30 synchronously turns on its illumination, ensuring that all N channels operate simultaneously without time delay. The illumination light from all channels simultaneously illuminates their respective color rings, and each color receiving fiber synchronously receives the reflected light signal, thereby achieving parallel data acquisition and shortening the single recognition cycle.

[0039] After receiving N sets of RGB color data, the main control unit 40 combines them into a complete color wheel code and decodes and identifies the parameter information of the microscope objective according to the pre-stored color wheel code and objective parameter mapping table. In an optional embodiment, the main control unit 40 is an STM32 microcontroller.

[0040] Example 2 like Figure 2As shown, this embodiment provides a microscope objective lens, employing the automatic microscope objective lens identification system provided in this embodiment of the invention. The rear end face of the microscope objective lens has two or more color-coded rings coaxial with the objective lens. Each color ring is arranged concentrically in the radial direction, and the plane containing each color ring is perpendicular to the central axis of the objective lens. The center of each color ring is located on the central axis of the objective lens. The color rings are arranged sequentially from the inside to the outside in the radial direction, with a radial distance of 0.5 mm between adjacent color rings. The color combination of each color ring is used to characterize the parameter information of the microscope objective lens.

[0041] Each color ring has a single, fixed color. Color rings at different radial positions may use the same or different colors. The color combination of each color ring—including the colors of each color ring and their radial arrangement order—uniquely represents the parameter information of the microscope objective. The parameter information includes at least one of the following: objective magnification, numerical aperture (NA), parfocal distance, objective type, and production batch.

[0042] The aforementioned color rings are formed on the rear end surface of the microscope objective using a high-temperature ceramic coloring process. This method results in color rings with excellent resistance to organic solvents, allowing them to withstand repeated wiping with common microscope cleaning solvents such as alcohol, acetone, and xylene without fading, peeling, or damage. This ensures the color stability and reliable identification of the color rings during long-term use and routine maintenance of the microscope.

[0043] This invention allows for tiered settings of the number of channels and colors in the color rings based on different application scenarios and recognition requirements: In terms of the number of channels, at least two rings are set to form a multi-level combination code. For conventional biological microscope scenarios, three rings are preferred (e.g., a 3-channel 6-color scheme provides 216 coding capacities). For industrial scenarios involving the mixing of multiple objective lenses, four rings are preferred (e.g., a 4-channel 4-color scheme provides 256 coding capacities). The upper limit of the number of channels is limited by the radial space of the rear end face of the objective lens and generally does not exceed six. In terms of colors, each ring can be selected for at least two colors, preferably four to six highly distinguishable pure colors (e.g., red, green, blue, and yellow).

[0044] For microscope objective identification systems used in standard biological microscopes, three concentric color rings are arranged on the rear end face of the microscope objective. Each ring can be selected from six pure colors: black, red, yellow, green, blue, and white. The colors of the rings can be the same or different. The color combination—including the colors of each ring and their radial arrangement—uniquely represents a single objective parameter. The theoretical number of coding combinations is 6³ = 216, sufficient to cover all common biological microscope objective models and parameters. A specific coding example is shown below: Objective lens 1 (4×, NA 0.10) is coded as ring 1 (red), ring 2 (black), and ring 3 (black); Objective lens 2 (10×, NA 0.25) is coded as ring 1 (yellow), ring 2 (black), and ring 3 (black); Objective lens 3 (40×, NA 0.65) is coded as ring 1 (green), ring 2 (black), and ring 3 (black); Objective lens 4 (100× oil immersion, NA 1.25) is coded as ring 1 white, ring 2 blue, and ring 3 black.

[0045] This embodiment adopts a 3-channel 6-color encoding scheme, which simplifies the color recognition algorithm while ensuring sufficient encoding capacity, and improves recognition speed and reliability. It is suitable for automatic identification of objectives in conventional biological microscopes.

[0046] For microscope objective recognition systems used in industrial inspection, metallurgical microscopes, and fluorescence objectives, a four-ring concentric color scheme is used on the rear end face of the microscope objective. Each ring can be selected from four high-discrimination pure colors: red, green, blue, and yellow, theoretically providing four possible coding combinations. 4 =256 types, which can meet the needs of mixed identification of various specifications of objectives in industrial inspection.

[0047] Example 3 like Figure 3 As shown, this embodiment provides an automatic identification method for microscope objectives, including the following steps: S10. Stable illumination light is output to the rear end face of the microscope objective through an illumination optical fiber. The rear end face of the microscope objective is provided with a color ring encoding disk, which includes N color rings. Stable illumination light is output to N color rings on the rear end face of the microscope objective through N illumination optical fibers, each illumination optical fiber corresponding to a color ring, and the illumination light is perpendicularly illuminating the surface of its corresponding color ring; wherein, N takes the value of 2, 3 or 4.

[0048] Each illumination fiber corresponds one-to-one with each color ring: the first illumination fiber points to the first color ring, the second illumination fiber points to the second color ring, and so on, with the Nth illumination fiber pointing to the Nth color ring. Each illumination fiber independently illuminates one color ring, eliminating the problems of overlapping light spot coverage or signal crosstalk between channels. Each channel can independently optimize the light spot position and focusing state, resulting in high color reading accuracy.

[0049] Please refer to the following at the same time Figure 1 Each illumination fiber 21 is integrated inside each coaxial fiber 20, and one end of each illumination fiber is connected to the light source output port of each color recognition sensor, while the other end points to its corresponding color ring. The color recognition sensor 30 is connected to the coaxial fiber 20 in a one-to-one correspondence, and the illumination light emitted by the color recognition sensor 30 is transmitted to the corresponding color ring on the color ring encoding disk 11 through its corresponding illumination fiber.

[0050] S20. Receive the reflected light signal carrying color information after being reflected by the color ring encoding disk through the color receiving optical fiber; Specifically, N color receiving optical fibers are used to receive the reflected light signals from each color ring, with each color receiving optical fiber corresponding to a color ring; each color receiving optical fiber receives the reflected light signal from its corresponding color ring.

[0051] like Figure 1 As shown, each color receiving fiber 22 is integrated inside each coaxial fiber 20, and each coaxial fiber 20 integrates one illumination fiber 21 and one color receiving fiber 22. The color receiving fiber 22 is connected to the optical signal input port of the corresponding color recognition sensor.

[0052] After the color receiving optical fiber 22 captures the reflected light signal reflected by the corresponding color ring, it transmits the reflected light signal along the inside of the optical fiber to the corresponding color sensor.

[0053] S30. Acquire the reflected light signal through a color recognition sensor and convert it into RGB color data; Specifically, N color recognition sensors acquire each reflected light signal, and each color recognition sensor converts its received reflected light signal into RGB color data, outputting a total of N sets of RGB color data.

[0054] The N color sensors correspond one-to-one with the N color rings. Each color recognition sensor independently provides illumination light to its corresponding color ring and receives the reflected light signal reflected by that color ring.

[0055] S40. The main control unit receives the RGB color data and decodes and identifies the parameter information of the microscope objective according to the pre-stored color wheel encoding and objective parameter mapping table.

[0056] The main control unit receives the N sets of RGB color data and combines them into a complete color wheel code. The main control unit 40 reads the pre-stored color wheel code and objective lens parameter mapping table in its internal memory (such as Flash or EEPROM). In this invention, the color wheel code and objective lens parameter mapping table is stored in key-value pair format, where the "key" is the code value of each color wheel color combination, and the "value" is the objective lens parameter information corresponding to that code.

[0057] The mapping table is constructed based on the principle that each color combination on the color wheel—including the types of colors on each color wheel and their radial arrangement—has a unique correspondence with the objective lens parameters. The combinations of different color wheel coding disks are distinct, ensuring that each objective lens corresponds to a unique set of color codes.

[0058] The main control unit 40 compares the received RGB color data with each entry in the mapping table, using a minimum color difference matching algorithm—that is, calculating the color difference ΔE between the measured color value and each standard color value in the mapping table, and selecting the entry with the smallest ΔE as the matching result—to identify the current microscope objective's magnification, numerical aperture (NA), parfocal distance, objective type, and production batch, among other parameters. If a match is found, the corresponding microscope objective's parameter information is extracted and output; if no match is found, default parameters are output or an error alarm is triggered.

[0059] Furthermore, after step S40, the following steps are also included: S50. Based on the parameter information of the microscope objective, automatically adapt and adjust at least one of the microscope's scale graduations, exposure parameters, light source brightness, and focusing parameters.

[0060] After decoding and recognizing the objective lens parameters, the main control unit 40 generates corresponding control commands based on the recognition results, and automatically adapts and adjusts various operating parameters of the microscope through the corresponding drive circuits and actuators.

[0061] The specific adjustment items include at least one of the following: (a) Adjustment of scale calibration coefficient (pixel equivalent); The main control unit 40 automatically calculates the pixel equivalent (i.e., the actual physical size corresponding to each pixel) in the current field of view based on the identified objective magnification. For example, the pixel equivalent for a 10× objective is 1.2 μm / pixel, for a 40× objective it is 0.3 μm / pixel, and for a 100× objective it is 0.12 μm / pixel. The main control unit 40 writes this calibration coefficient into the scale module of the microscope image acquisition software to ensure that the measurement scale of the image overlay accurately corresponds to the actual size, eliminating the need for manual calibration.

[0062] (b) Automatic adjustment of exposure parameters; Objective lenses with different magnifications have different numerical apertures and light throughputs. High-magnification objectives have large numerical apertures but lower field-of-view brightness. The main control unit 40 automatically adjusts the exposure time and gain value of the camera or image sensor according to the objective lens parameters to keep the image brightness within the optimal dynamic range (e.g., the value in the grayscale histogram is in the range of 128±16), avoiding overexposure or underexposure of the image.

[0063] (c) Automatic adjustment of light source brightness; The main control unit 40 automatically adjusts the output power of the microscope illumination source (such as an LED illuminator or halogen lamp power supply) according to the objective magnification. Low magnification objectives (such as 4×, 10×) require stronger illumination to cover a larger field of view, while high magnification objectives (such as 60×, 100×) require lower illumination to avoid sample overheating or photobleaching, while obtaining the best image contrast.

[0064] (d) Automatic compensation of focus parameters; The parfocal distance of different objectives may vary slightly. When the user switches to a different objective, the main control unit 40 automatically drives the Z-axis stepper motor or piezoelectric ceramic driver to perform micron-level focusing compensation based on the identified objective parameters and the preset parfocal compensation value. This allows the sample to quickly enter the focal plane, shortens the focusing time after changing the objective, and improves operational efficiency.

[0065] In addition, the main control unit 40 can also upload the identified objective lens parameter information to the host computer (such as PC image acquisition software) via the communication line 41, and display the current objective lens magnification and parameter information in real time in the software interface, which is convenient for operators to view and record.

[0066] It should be understood that the various forms of processes shown above can be used to reorder, add, or delete steps. For example, the steps described in this invention disclosure can be executed in parallel, sequentially, or in different orders, as long as the desired result of the technical solution disclosed in this invention can be achieved, and this is not limited herein.

[0067] The specific embodiments described above do not constitute a limitation on the scope of protection of this invention. Those skilled in the art should understand that various modifications, combinations, sub-combinations, and substitutions can be made according to design requirements and other factors. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of this invention should be included within the scope of protection of this invention.

Claims

1. An automatic identification system for microscope objectives, characterized in that, include: The microscope objective lens has a color ring coding disk on its rear end face; A coaxial optical fiber, one end of which points to the color ring encoding disk and the other end of which is connected to a color recognition sensor, is used to transmit illumination light to the color ring encoding disk of the microscope objective and to acquire reflected light signals carrying color information. The color recognition sensor is used to acquire the reflected light signal and convert it into RGB color data; The main control unit is used to receive the RGB color data and decode and identify the parameter information of the microscope objective according to the pre-stored color wheel encoding and objective parameter mapping table.

2. The automatic microscope objective recognition system according to claim 1, characterized in that: The coaxial optical fiber integrates an illumination fiber and a color receiving fiber. The illumination fiber is connected to the light source output port of the color recognition sensor, and the color receiving fiber is connected to the light signal input port of the same color recognition sensor.

3. The automatic microscope objective recognition system according to claim 1, characterized in that, The color ring encoder disk includes N color rings, and the N color rings are arranged concentrically in a radial direction, where N is 2, 3 or 4.

4. The automatic microscope objective recognition system according to claim 3, characterized in that, The coaxial optical fiber consists of N fibers, each corresponding to one of the N color rings, with one end of each fiber pointing to its corresponding color ring.

5. The automatic microscope objective recognition system according to claim 4, characterized in that, There are N color recognition sensors, each connected to one of the N coaxial optical fibers.

6. The automatic microscope objective recognition system according to claim 1, characterized in that, The color ring code and objective lens parameter mapping table records the objective lens parameter information corresponding to each color ring code disk. The objective lens parameter information includes at least one of the following: objective lens magnification, numerical aperture (NA), objective lens type, and production batch.

7. A microscope objective lens, applied to the automatic microscope objective lens recognition system according to any one of claims 1 to 6, characterized in that: The microscope objective is provided with a color ring coding disk on its rear end face, which is used to characterize the parameter information of the microscope objective.

8. An automatic identification method for microscope objectives, characterized in that, Including the following steps: S10. Stable illumination light is output to the rear end face of the microscope objective through an illumination optical fiber. The rear end face of the microscope objective is provided with a color ring encoding disk, which includes N color rings. S20. Receive the reflected light signal carrying color information after being reflected by the color ring encoding disk through the color receiving optical fiber; S30. Acquire the reflected light signal through a color recognition sensor and convert it into RGB color data; S40. The main control unit receives the RGB color data and decodes and identifies the parameter information of the microscope objective according to the pre-stored color wheel encoding and objective parameter mapping table.

9. The automatic identification method for microscope objectives according to claim 8, characterized in that, In step S10, there are N lighting optical fibers, and the illumination output from the N lighting optical fibers is respectively perpendicularly illuminating the N color rings.

10. The automatic identification method for microscope objectives according to claim 8, characterized in that, After step S40, the method further includes: S50. Based on the parameter information of the microscope objective, automatically adapt and adjust at least one of the microscope's scale graduations, exposure parameters, light source brightness, and focusing parameters.

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