Detachable PCR reaction tube support

By designing a detachable PCR reaction tube bracket, the problem of the inability to flexibly adjust the bracket design in the existing technology is solved, and the flexible combination of the full-page machine-mounted experiment and multiple eight-connected tube brackets is realized, which improves the experimental efficiency and operation convenience, and meets the needs of high-throughput experiments.

CN223060948UActive Publication Date: 2025-07-04JIHE LIFE SCI & TECH (DONGGUAN) CO LTD
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Patent Information

Application Number
CN202421541844.2
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-07-02
Publication Date
2025-07-04
Estimated Expiration
2034-07-02

AI Technical Summary

Technical Problem

The existing PCR reaction tube bracket design cannot meet the flexibility of the whole version and the need to disassemble it into multiple eight-connected tube brackets, resulting in complex and inefficient experimental operations, which cannot meet the needs of high-throughput experiments.

Method used

A removable PCR reaction tube bracket is designed, including multiple eight-connected tube brackets, vertical support strip seats and transverse support seats. The fixed connection and flexible disassembly of the bracket are achieved through notches and easily broken connection points to form an integral bracket, which can be tested in the whole machine and can be detached into 1 to 12 eight-connected tube brackets.

Benefits of technology

It has achieved significant improvement in the convenience and efficiency of experimental operations, and can flexibly adjust the number and arrangement of reaction tubes according to experimental needs, avoid resource waste, and meet flexible combination use under different experimental conditions.

✦ Generated by Eureka AI based on patent content.

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Abstract

The utility model discloses a detachable PCR (Polymerase Chain Reaction) reaction tube bracket, aiming at solving the problems of complicated operation, low efficiency and insufficient flexibility of the PCR reaction tube bracket in the prior art. The support comprises a plurality of eight-connecting-pipe supports, a vertical supporting strip seat and a transverse supporting seat, and the eight-connecting-pipe supports are fixedly connected with one another through a plurality of notches and easy-to-break connecting points to form an eight-connecting-pipe support set. According to the utility model, the bracket can be used for full-page computer experiment, and can also be disassembled into 1-12 eight-connecting-pipe brackets which are flexibly combined for use, so that the convenience and the efficiency of experiment operation are remarkably improved. Through the detachable design, a PCR experiment can be more efficiently carried out in a laboratory, different experiment requirements are met, and resource waste is avoided.
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Description

Technical Field

[0001] The utility model relates to the technical field of molecular biology experimental equipment, and in particular to a detachable PCR reaction tube holder. Background Art

[0002] The PCR (Polymerase Chain Reaction) technology is widely used in the field of molecular biology for amplifying specific DNA fragments. However, there are some problems in the design of existing PCR reaction tube holders, which seriously affect the convenience and efficiency of experimental operations. For example:

[0003] 1. Traditional PCR reaction tube holders are usually divided into two types: separate eight-tube holders and full-plate PCR plates. The separate eight-tube holders need to be placed individually on the PCR machine for experiments, which not only increases the complexity and workload of experimental operations, but also easily leads to operation errors and affects the accuracy of experimental results. In addition, the method of placing them individually results in low experimental efficiency and cannot meet the needs of high-throughput experiments.

[0004] 2. Although the full-plate PCR plates can place multiple PCR reaction tubes at one time, their fixing structures lack flexibility. The full-plate PCR plates are not easy to adjust under specific experimental conditions and cannot meet the flexible needs of laboratories for different experimental combinations. For example, in some experiments that require a specific number of PCR reaction tubes, the full-plate PCR plates are too bulky and waste resources.

[0005] 3. In actual operations, experimental personnel often need to adjust the number and arrangement of PCR reaction tubes according to experimental requirements. The existing holder designs cannot achieve flexible adjustment, which not only increases the experimental preparation time, but also easily leads to waste of consumables. In addition, there is no PCR reaction tube holder on the market that can be used in a full-plate manner and can be flexibly disassembled into multiple eight-tube holders. This gap makes it inconvenient for laboratories to conduct efficient experiments.

[0006] For example, in a high-throughput gene detection experiment, experimental personnel need to process multiple samples simultaneously. When using traditional separate eight-tube holders, experimental personnel need to place multiple eight-tube holders individually, which is cumbersome and inefficient. When using full-plate PCR plates, it is difficult to adjust the number of PCR reaction tubes according to different sample numbers, and the optimal allocation of resources cannot be achieved.

[0007] Therefore, how to design a PCR reaction tube holder that can be used in a full-plate manner and can be flexibly disassembled into multiple eight-tube holders has become the technical problem to be solved by the utility model. Summary of the Utility Model

[0008] The technical problem solved by the present utility model is to provide a detachable PCR reaction tube holder to address the deficiencies in the above-mentioned prior art, so as to solve the problems of low flexibility and efficiency mentioned in the above background art.

[0009] To solve the above technical problems, the technical solution adopted by the present utility model is as follows:

[0010] A detachable PCR reaction tube holder includes multiple eight-tube holders, vertical support strip seats, and horizontal support seats. The eight-tube holders have tube placement limit holes. Multiple eight-tube holders are arranged side by side, and the sides of adjacent two eight-tube holders are fixedly connected to each other through multiple notches and easily breakable connection points to form an eight-tube holder group.

[0011] Both sides of the eight-tube holder group are fixedly connected to the vertical support strip seats through multiple notches and easily breakable connection points.

[0012] Horizontal support seats are fixedly arranged on the upper side and the lower side of a single eight-tube holder respectively.

[0013] As a further solution of the present utility model, the eight-tube holder group, the vertical support strip seats, and the horizontal support seats are combined to form an integral holder.

[0014] As a further solution of the present utility model, the integral holder is integrally formed by injection molding.

[0015] As a further solution of the present utility model, the eight-tube holder group is fixedly connected and formed by 12 eight-tube holders.

[0016] As a further solution of the present utility model, the 12 eight-tube holders can be detachably separated into any number of 1 - 12 eight-tube holders in a breakable manner through multiple notches and easily breakable connection points.

[0017] As a further solution of the present utility model, the easily breakable connection points include trapezoidal connecting columns. Trapezoidal connecting columns are fixedly arranged on both sides of the eight-tube holder. The trapezoidal connecting column includes a trapezoidal large bottom end and a trapezoidal small bottom end. The trapezoidal connecting column is fixedly connected to the eight-tube holder through the trapezoidal large bottom end, and the two trapezoidal connecting columns between adjacent two eight-tube holders are fixedly connected through the two trapezoidal small bottom ends.

[0018] As a further solution of the present utility model, the number of trapezoidal connecting columns is more than 3. After adjacent two eight-tube holders are connected through multiple easily breakable connection points, notches are formed between two easily breakable connection points.

[0019] As a further solution of the present utility model, the notch is a through-notch.

[0020] As a further solution of the present utility model, the tube placement limiting holes on the eight-tube holder are used to place eight-tubes of 0.2 ml model or 0.1 ml model.

[0021] Compared with the prior art, the beneficial effects of the present utility model are as follows:

[0022] 1. Multiple eight-tube holders are fixedly connected to form an eight-tube holder group, and through the connection of the notches and easily breakable connection points with the vertical support bar seat and the horizontal support seat, it can be realized that the whole plate can be used for experiments on the machine, and it can also be flexibly disassembled into 1 to 12 eight-tube holders, meeting the flexible combination and use requirements under different experimental conditions, and avoiding the operation complexity and resource waste brought by the fixed structure of the traditional bracket design.

[0023] 2. When conducting experiments in different batches, the experimenter can flexibly adjust the number of eight-tube holders according to needs. For example, when conducting tests on a small number of samples, the bracket can be disassembled into 1 or 2 eight-tube holders for experiments, avoiding resource waste. When large-scale sample testing is required, 1 to 12 eight-tube holders can be combined into a complete eight-tube holder group and placed on the PCR machine as a whole plate for high-throughput experiments, further improving the experimental efficiency.

[0024] 3. When the experimenter needs to perform PCR reactions on 24 samples simultaneously, the bracket can be disassembled into 3 eight-tube holders. Each eight-tube holder is connected to each other through multiple notches and easily breakable connection points to form an eight-tube holder group. Both sides of the eight-tube holder group are fixedly connected to the vertical support bar seat through multiple notches and easily breakable connection points. Horizontal support seats are fixedly arranged on the upper and lower sides of each eight-tube holder respectively to ensure the fixation and support of the reaction tubes during the experimental operation. At the same time, during this experimental process, the experimenter only needs to directly place the disassembled 3 eight-tube holder group on the PCR machine without placing the reaction tubes one by one, thus greatly simplifying the operation steps and improving the experimental efficiency.

[0025] The additional aspects and advantages of the present utility model will be partly given in the following description, partly will become obvious from the following description, or will be understood through the practice of the present utility model. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] In order to more clearly illustrate the technical solutions in the embodiments of the present utility model or the prior art, the following will briefly introduce the drawings required for use in the description of the embodiments or the prior art. Obviously, the following drawings are only some embodiments of the present utility model. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative efforts.

[0027] Figure 1 It is a structural schematic diagram of the present utility model.

[0028] Figure 2 is Figure 1 a structural schematic diagram from another perspective.

[0029] Figure 3 is a structural schematic diagram of the front side of the present utility model.

[0030] Figure 4 is Figure 3 a bottom view schematic diagram of

[0031] Figure 5 is Figure 4 an enlarged schematic diagram of part A of

[0032] The reference numerals and names in the figure are as follows:

[0033] Eight - tube bracket 1, vertical support bar seat 2, horizontal support seat 3, tube placement limit hole 4, notch 5, easily - breakable connection point 6, eight - tube bracket group 7, overall bracket 8, and trapezoidal connection column 9. Specific embodiments

[0034] Next, the technical solutions in the embodiments of the present utility model will be described clearly and completely. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all of the embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without making creative efforts shall fall within the protection scope of the present utility model.

[0035] Please refer to Figure 1—5, in the embodiment of the present utility model, a detachable PCR reaction tube holder includes a plurality of eight-tube holders 1, a vertical support strip seat 2, and a horizontal support seat 3. Each eight-tube holder 1 has 8 tube placement limit holes 4 for correspondingly placing eight-tube reaction tubes, which all belong to the extended implementation methods known to those of ordinary skill in the art. The plurality of eight-tube holders 1 are arranged side by side, and the sides of adjacent two eight-tube holders 1 are fixedly connected to each other through a plurality of notches 5 and easily breakable connection points 6 to form an eight-tube holder group 7; both sides of the eight-tube holder group 7 are fixedly connected to the vertical support strip seat 2 through a plurality of notches 5 and easily breakable connection points 6; the upper side and the lower side of a single eight-tube holder 1 are respectively fixedly provided with a horizontal support seat 3. The eight-tube holder group 7, the vertical support strip seat 2, and the horizontal support seat 3 are combined to form an overall holder 8. The overall holder 8 is integrally formed by injection molding, avoiding the problems of loosening or displacement of the reaction tubes during operations such as high-speed centrifugation, thereby ensuring the accuracy and repeatability of the experimental results. Through this design, when performing high-throughput gene detection or other PCR experiments, experimenters do not need to place the reaction tubes one by one, reducing the operation steps and the risk of operation errors; and, after breaking, each single eight-tube holder 1 can also be supported by two horizontal support seats 3, which all belong to the extended implementation methods known to those of ordinary skill in the art.

[0036] The eight-tube holder group 7 is fixedly connected and formed by 12 eight-tube holders 1. The 12 eight-tube holders 7 can be detachably separated into any number of eight-tube holders 1 from 1 to 12 in a breaking manner through a plurality of notches 5 and easily breakable connection points 6. The easily breakable connection point 6 includes a trapezoidal connecting column 9. Trapezoidal connecting columns 9 are respectively fixedly provided on both sides of the eight-tube holder 1. The trapezoidal connecting column 9 includes a trapezoidal large bottom end and a trapezoidal small bottom end. The trapezoidal connecting column 9 is fixedly connected to the eight-tube holder 1 through the trapezoidal large bottom end. The two trapezoidal connecting columns 9 between adjacent two eight-tube holders 1 are fixedly connected through two trapezoidal small bottom ends. The number of trapezoidal connecting columns 9 is more than 3. After adjacent two eight-tube holders 1 are connected through a plurality of easily breakable connection points 6, a notch 5 is formed between two easily breakable connection points 6. The notch 5 is a through-type notch 5. The tube placement limit holes 4 on the eight-tube holder 1 are used for placing 0.2 ml or 0.1 ml eight-tube reaction tubes.

[0037] Example 1:

[0038] A detachable PCR reaction tube holder includes a plurality of eight-tube holders 1, a vertical support strip seat 2, and a horizontal support seat 3.

[0039] In a specific implementation, multiple eight-tube holders 1 are fixedly connected to each other through multiple notches 5 and easily breakable connection points 6 to form an eight-tube holder group 7. Each eight-tube holder 1 is composed of 8 PCR reaction tubes, and the eight-tube holder group 7 can be formed by combining 1 to 12 eight-tube holders 1. The two sides of the eight-tube holder group 7 are respectively fixedly connected to the vertical support bar seats 2 through multiple notches 5 and easily breakable connection points 6. Horizontal support seats 3 are fixedly arranged on the upper side and the lower side of a single eight-tube holder 1 respectively to ensure the fixation and support of each reaction tube during experimental operations.

[0040] This design solves multiple problems in the prior art. First, through the connection design of multiple eight-tube holders 1, the whole-plate loading experiment operation is realized, and there is no need to place PCR reaction tubes one by one, significantly improving the experimental efficiency. Second, since the eight-tube holder group 7 can be flexibly disassembled into any number of eight-tube holders 1, it meets the needs of different experimental combinations in the laboratory and provides a high degree of flexibility. For example, in a high-throughput gene detection experiment, the experimenter can select an appropriate number of eight-tube holder groups 7 according to the number of samples for the experiment, avoiding resource waste and having a simple operation.

[0041] Application example: In a high-throughput gene detection experiment, the experimenter needs to process 96 samples. Using the PCR reaction tube holder of the present utility model, the experimenter can select an eight-tube holder group 7 combined by 12 eight-tube holders 1 and place the whole plate on the PCR machine for the experiment. The whole experimental operation process is simple and fast, avoiding the cumbersome operation of placing reaction tubes one by one and significantly improving the experimental efficiency.

[0042] By adopting this detachable PCR reaction tube holder, the laboratory can carry out PCR experiments more efficiently, meet different experimental needs, avoid resource waste, and improve the convenience of experimental operations and the reliability of results. This design shows significant advantages in practical applications and solves the key problems in the prior art.

[0043] Embodiment 2:

[0044] In actual experimental operations, the detachable PCR reaction tube holder can not only be loaded onto the machine as a whole plate for experiments, but also be flexibly disassembled into multiple eight-tube holders 1 to meet different experimental needs. For example, in a gene expression analysis experiment, the experimenter needs to perform PCR reactions on different numbers of samples simultaneously according to the experimental design. In this case, the detachable PCR reaction tube holder provided by the present utility model demonstrates its significant advantages.

[0045] In specific implementation, when an experimenter needs to perform PCR reactions on 24 samples simultaneously, the bracket can be disassembled into three eight-tube brackets 1. Each eight-tube bracket 1 is connected to each other through a plurality of notches 5 and easily breakable connection points 6 to form an eight-tube bracket group 7. Both sides of the eight-tube bracket group 7 are fixedly connected to the vertical support bar seats 2 through a plurality of notches 5 and easily breakable connection points 6. Horizontally support seats 3 are fixedly arranged on the upper side and the lower side of each eight-tube bracket 1 respectively.

[0046] In this experimental process, the experimenter only needs to directly place the eight-tube bracket group 7 formed by the three disassembled eight-tube brackets 1 onto the PCR machine, without placing the reaction tubes one by one, thus greatly simplifying the operation steps and improving the experimental efficiency.

[0047] In addition, when conducting experiments in different batches, the experimenter can flexibly adjust the number of eight-tube brackets 1 according to needs. For example, when conducting tests on a small number of samples, the bracket can be disassembled into one or two eight-tube brackets 1 for experiments, avoiding waste of resources. When large-scale sample testing is required, the eight-tube bracket group 7 formed by combining one to twelve eight-tube brackets 1 can be placed entirely onto the PCR machine for high-throughput experiments, further improving the experimental efficiency.

[0048] After adopting the detachable PCR reaction tube bracket of the present utility model, the laboratory can not only significantly improve the convenience and efficiency of experimental operations, but also flexibly adjust the number and arrangement of reaction tubes according to experimental requirements, meeting the diverse needs of the laboratory.

[0049] In the present utility model, unless otherwise clearly specified and defined, terms such as "installation", "setting", "connection", "fixation", "rotation connection", etc. should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or integrated; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium. It can be the internal communication of two components or the interaction relationship between two components. Unless otherwise clearly defined, for those of ordinary skill in the art, the specific meanings of the above terms in the present utility model can be understood according to specific situations.

[0050] For those skilled in the art, it is obvious that the present utility model is not limited to the details of the above exemplary embodiments, and can be implemented in other specific forms without departing from the spirit or basic characteristics of the present utility model. Therefore, from any point of view, the embodiments should be regarded as exemplary and non-restrictive. The scope of the present utility model is defined by the appended claims rather than the above description. Therefore, it is intended to include all changes falling within the meaning and scope of the equivalent elements of the claims in the present utility model.

Claims

1. A detachable PCR reaction tube holder, comprising a plurality of eight-tube holders, a vertical support strip seat and a horizontal support seat. The eight-tube holder has a tube placement limit hole. It is characterized in that: A plurality of eight-tube holders are arranged side by side, and the sides of adjacent two eight-tube holders are fixedly connected to each other through a plurality of notches and easily breakable connection points to form a group of eight-tube holders; Both sides of the group of eight-tube holders are fixedly connected to the vertical support strip seat through a plurality of notches and easily breakable connection points; The upper side and the lower side of a single eight-tube holder are respectively fixedly provided with a horizontal support seat.

2. The detachable PCR reaction tube holder according to claim 1, characterized in that, The group of eight-tube holders, the vertical support strip seat and the horizontal support seat are combined to form an integral holder.

3. The detachable PCR reaction tube holder according to claim 2, wherein, The integral holder is integrally formed by injection molding.

4. A detachable PCR reaction tube holder according to claim 1, characterized in that, The group of eight-tube holders is fixedly connected and formed by 12 eight-tube holders.

5. The detachable PCR reaction tube holder according to claim 4, characterized in that, The 12 eight-tube holders can be detachably separated into any number of 1-12 eight-tube holders in a breaking way through a plurality of notches and easily breakable connection points between each other.

6. A detachable PCR reaction tube holder according to claim 1, wherein The easily breakable connection point includes a trapezoidal connection column. Trapezoidal connection columns are respectively fixedly provided on both sides of the eight-tube holder. The trapezoidal connection column includes a trapezoidal large bottom end and a trapezoidal small bottom end. The trapezoidal connection column is fixedly connected to the eight-tube holder through the trapezoidal large bottom end. The two trapezoidal connection columns between adjacent two eight-tube holders are fixedly connected through two trapezoidal small bottom ends.

7. A detachable PCR reaction tube holder according to claim 6, wherein, The number of trapezoidal connection columns is more than 3. After adjacent two eight-tube holders are connected through a plurality of easily breakable connection points, a notch is formed between two easily breakable connection points.

8. A detachable PCR reaction tube holder according to claim 7, wherein The notch is a through-type notch.

9. A detachable PCR reaction tube holder according to claim 7, characterized in that, The tube placement limit hole on the eight-tube holder is used for placing 0.2 ml model or 0.1 ml model eight-tubes.

Citation Information

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