Automatic low-temperature valve

Abstract

The utility model relates to an automatic low-temperature valve which comprises a valve seat. A liquid inlet connector is installed on one side of the valve seat, a plurality of liquid injection steel needles are installed at the bottom of the valve seat in an arrayed mode, a plurality of medium isolation valves are installed at the top of the valve seat in an arrayed mode, and one medium isolation valve corresponds to one liquid injection steel needle. A medium channel is arranged in the valve seat, and the liquid inlet connector is communicated with the liquid injection steel needle through the medium channel via the medium isolation valve. Cooling connectors are arranged on the two sides of the valve seat, a cooling channel is arranged in the valve seat, and the cooling channel is isolated from the medium channel. The cooling channel is wound around the medium channel; one end of the cooling channel is a refrigerant inlet, and the other end of the cooling channel is a refrigerant outlet; the refrigerant inlet connector and the refrigerant outlet connector are connected with cooling circulation equipment through pipelines, and refrigerants are introduced into the cooling channel. The utility model has the advantages of simple structure and strong usability; by adjusting the temperature of the refrigerant, the temperature of the valve seat, namely the medium channel, can be controlled so as to meet the adding environment temperature requirements of reagents with different temperatures, automatic adding is facilitated, and the production efficiency is improved.

Description

Technical Field

[0001] The utility model relates to a valve device for liquid transfer, in particular to a valve that can stably operate at low temperatures. Background Art

[0002] In the field of gene synthesis, it is necessary to add reagents such as enzymes to reagents for operation. However, the required environment for the reagents is relatively low, generally below 8°C. During the addition process, a valve is often needed to control the amount of the reagent. However, it is difficult for the equipment to reach a low temperature in a normal temperature environment, and it is expensive to operate as a whole in a low-temperature chamber.

[0003] In the existing operation, due to the need to maintain a low temperature, only manual equipment can be selected. First, it is refrigerated at a low temperature, and then manual operation is carried out quickly. After several operations, when the temperature rises, a low-temperature device is replaced and the operation is continued. This is rather troublesome.

[0004] How to achieve automatic addition while ensuring a low-temperature operating environment requires structural improvement of the valve device. Summary of the Invention

[0005] The purpose of the utility model is to provide an automatic low-temperature valve. A cooling channel is arranged in the valve seat, and a low-temperature refrigerant is introduced to cool the valve seat in real time. Moreover, the cooling channel bypasses the medium channel, so that the reagent is always in a low-temperature environment, meeting the operation requirements of automatic low-temperature addition of enzyme reagents, improving the operation efficiency, and reducing the waste of enzyme reagents.

[0006] To achieve the above-mentioned utility model purpose, the utility model provides an automatic low-temperature valve, including a valve seat;

[0007] A liquid inlet interface is installed on one side of the valve seat, and a number of liquid injection steel needles are arranged and installed at the bottom of the valve seat. A number of medium isolation valves are arranged and installed at the top of the valve seat, and one medium isolation valve corresponds to one liquid injection steel needle;

[0008] A medium channel is arranged in the valve seat, and the liquid inlet interface is connected to the liquid injection steel needle through the medium channel via the medium isolation valve;

[0009] Cooling interfaces are arranged on both sides of the valve seat, and a cooling channel is arranged in the valve seat. The cooling channel is isolated from the medium channel; the cooling channel bypasses the medium channel;

[0010] One end of the cooling channel is a refrigerant inlet interface, and the other end is a refrigerant outlet interface; the refrigerant inlet interface and the refrigerant outlet interface are connected to a cooling circulation device through pipelines, and a refrigerant is passed through the cooling channel.

[0011] As a further improvement of the utility model, a horizontal liquid inlet flow channel is arranged on the front side of the valve seat, the liquid inlet interface is installed at the end of the liquid inlet flow channel, and the liquid inlet interface is connected to a reagent barrel through a pipeline;

[0012] Vertically inside the valve seat, there are two columns of channels. In the front side, there are several inlet valve channels, and in the rear side, there are several liquid injection channels.

[0013] One inlet valve channel and one liquid injection channel form a group and are connected to a medium isolation valve.

[0014] On both sides of a group of inlet valve channels and liquid injection channels, there are mounting screw holes for fixing the mounting screws of the medium isolation valve.

[0015] Furthermore, a distribution channel is provided between the liquid inlet channel and the inlet valve channel, and the distribution channel is horizontally arranged.

[0016] Still further, the liquid inlet channel is located in the middle of the distribution channel, and several inlet valve channels are evenly distributed upward from the distribution channel.

[0017] Still further, the aperture of the inlet valve channel is smaller than the diameter of the distribution channel.

[0018] As a further improvement of the present utility model, the cooling channel is provided with three horizontally penetrating cooling channels in the valve seat, namely the first cooling channel, the second cooling channel, and the third cooling channel.

[0019] Furthermore, the first cooling channel and the second cooling channel are located at the rear of the valve seat. The first cooling channel and the second cooling channel are vertically distributed, and are connected by a vertical channel between them.

[0020] The first cooling channel is located below, and one end is provided with a refrigerant inlet and is equipped with a refrigerant inlet interface.

[0021] The vertical channel is far from the refrigerant inlet interface.

[0022] Furthermore, the second cooling channel and the third cooling channel are located at the upper part of the valve seat. The second cooling channel and the third cooling channel are horizontally distributed, and are connected by a horizontal channel between them.

[0023] The third cooling channel is located in the medium channel, and one end of the third cooling channel is provided with a refrigerant outlet and is equipped with a refrigerant outlet interface.

[0024] The horizontal channel is far from the refrigerant outlet interface.

[0025] When the automatic low-temperature valve of the present utility model is in use, first start the refrigerant in advance, and inject the refrigerant below 4°C into the cooling channel in the valve seat.

[0026] The refrigerant is circulating cooling water. After being cooled to 4°C, it is pumped into the cooling channels in the valve seat through the refrigerant inlet interface, and flows through the first cooling channel, the vertical flow channel, the second cooling channel, the horizontal flow channel, and the third cooling channel, flowing everywhere in the valve seat, especially around the distribution channel, the inlet valve channel, and the liquid injection channel, cooling and lowering the temperature around the medium channel, so that the temperature of the valve seat is lower than 8°C, meeting the usage requirements of low-temperature enzyme reagents. Finally, it is output through the refrigerant outlet interface and then recycled back into the refrigeration equipment after refrigeration.

[0027] After the refrigerant operates for a period of time, the internal temperature of the valve seat is reduced from room temperature to below 8°C; then it starts to work. The automatic low-temperature valve of the present invention is used for automatically separating and adding reagents such as enzymes.

[0028] Start the pipeline of the low-temperature enzyme reagent. The reagent enters the medium channel in the valve seat through the separation pipeline and the liquid inlet interface, first flows into the distribution channel through the liquid inlet channel, flows to both sides, then flows upward into the fine holes of the inlet valve channel, is controlled by the medium isolation valve, and then flows downward into the liquid injection channel, and finally flows through the liquid injection steel needle and is injected into the test tube of the target well plate.

[0029] The automatic low-temperature valve of the present invention can be set by a program to achieve automatic addition of enzyme reagents. Basically, it can complete the enzyme addition of a 96-well plate in 48 seconds, and the automatic addition efficiency is high.

[0030] During the process of automatically adding enzyme reagents, the refrigerant continuously circulates and works to cool the valve seat, so that the temperature inside the valve island cavity is below 8°C, ensuring the quality of the enzyme reagents in the valve seat, and improving the success rate and output efficiency of gene synthesis.

[0031] The automatic low-temperature valve of the present invention first cools and lowers the temperature of the valve seat, so that the low-temperature enzyme reagent is always in a low-temperature state, thus reducing the waste of enzyme reagents and saving costs.

[0032] The automatic low-temperature valve of the present invention, through the flow channel design in the valve seat, that is, the distribution of the medium channel, realizes the separated output of multiple medium channels, can inject liquid into multiple test tubes at one time, and improves the addition efficiency; at the same time, cooling channels are distributed around the medium channel, and refrigerant is injected into them to cool the valve seat, especially the medium channel, so that the low-temperature enzyme medium is always in a cold environment, improving the reliability of the medium.

[0033] The automatic low-temperature valve of the present invention has a simple structure and strong usability; by adjusting the temperature of the refrigerant, the temperature of the valve seat and the medium channel can be controlled to meet the ambient temperature requirements for adding reagents at different temperatures, facilitating the automatic addition operation requirements and improving production efficiency. Description of the Drawings

[0034] Figure 1Schematic diagram of the overall structure of the automatic cryogenic valve of the present utility model Figure 1 ;

[0035] Figure 2 Schematic diagram of the overall structure of the automatic cryogenic valve of the present utility model Figure 2 ;

[0036] Figure 3 Overall structure external view of the valve seat of the present utility model;

[0037] Figure 4 Schematic diagram of the lower cross-section of the valve seat of the present utility model;

[0038] Figure 5 Schematic diagram of the upper cross-section of the valve seat of the present utility model;

[0039] Figure 6 Schematic diagram of the medium channel inside the valve seat of the present utility model;

[0040] Figure 7 Schematic diagram of the structure of a group of solenoid valves;

[0041] Figure 8 Schematic diagram of the longitudinal section of the rear part of the valve seat of the present utility model

[0042] Figure 9 Schematic diagram of the cooling channel inside the valve seat of the present utility model. Specific implementation manners

[0043] The following further elaborates on the specific implementation manners of the present utility model in conjunction with the accompanying drawings.

[0044] The automatic cryogenic valve of the present utility model has an overall structure as shown in Figure 1 , Figure 2 , and includes a valve seat 1. A liquid inlet interface 2 is installed on one side of the valve seat 1. A number of liquid injection steel needles 3 are arranged and installed at the bottom of the valve seat 1. A number of medium isolation valves 4 are arranged and installed at the top of the valve seat 1, and 1 medium isolation valve 4 corresponds to 1 liquid injection steel needle 3. A medium channel is provided inside the valve seat 1, and the liquid inlet interface 2 is connected to the liquid injection steel needle 3 through the medium channel via the medium isolation valve 4. Cooling interfaces 5 are provided on both sides of the valve seat 1. A cooling channel is provided inside the valve seat 1, and the cooling channel is isolated from the medium channel. The two ends of the cooling channel are respectively provided with cooling interfaces 5, one end is a refrigerant inlet interface 51, and the other end is a refrigerant outlet interface 52. A number of plugs 6 are also installed on the valve seat 1 to block the process ports of the medium channel and the cooling channel.

[0045] The key point of the present utility model is to design the structures of the medium channel and the cooling channel of the valve seat 1.

[0046] The specific design of the medium channel is as shown in Figure 3 , Figure 4 ,Figure 5 , Figure 6 , Figure 7 As shown in Figure 7 , a liquid inlet flow channel 11 is provided on the front side of the valve seat 1, and a liquid inlet interface 2 is installed at the end for connection with a reagent barrel; two columns of channels are provided in the vertical direction inside the valve seat 1. One column on the front side is a plurality of inlet valve channels 13, and one column on the rear side is a plurality of liquid injection channels 14; one inlet valve channel 13 and one liquid injection channel 14 form a group and are connected to one medium isolation valve 4. Mounting screw holes 15 are provided on both sides of a group of inlet valve channels 13 and liquid injection channels 14 for fixing the mounting screws 44 of the medium isolation valve 4.

[0047] In this utility model, only one liquid inlet flow channel 11 is provided, but a plurality of inlet valve channels 13 (8 in the attached drawings of this embodiment) are provided. Therefore, a distribution flow channel 12 is provided between the liquid inlet flow channel 11 and the inlet valve channels 13. The distribution flow channel 12 is horizontally arranged, and distribution plugs 122 are provided at both ends for installing plugs 6 for sealing. Preferably, the liquid inlet flow channel 11 is located in the middle of the distribution flow channel 12, and a plurality of inlet valve channels 13 are evenly distributed upward from the distribution flow channel 12.

[0048] The inlet valve channel 13 is a fine hole with a diameter smaller than that of the distribution flow channel 12, so as to ensure that the flow velocity and flow rate flowing to each medium isolation valve 4 are basically the same; correspondingly, the upper end of the liquid injection channel 14 connected to the medium isolation valve 4 is a fine hole to match the inlet valve channel 13, and a liquid injection steel needle 3 is installed at the bottom of the liquid injection channel 14, and the inside of the liquid injection steel needle 3 is also a fine hole.

[0049] The automatic low-temperature valve of this utility model is used for automatically separating and adding reagents such as enzymes. The liquid inlet interface 2 is connected to a reagent barrel through a pipeline. The reagent enters the medium channel inside the valve seat 1 through the liquid inlet interface 2, first flows into the distribution flow channel 12 through the liquid inlet flow channel 11, flows to both sides, and then flows upward into the fine holes of the inlet valve channels 13. Controlled by the medium isolation valve 4, and then flows downward into the liquid injection channels 14, and finally flows through the liquid injection steel needles 3 and is injected into the test tubes of the target well plate.

[0050] To realize the operation of the reagent in a low-temperature environment, a cooling channel is also provided inside the valve seat 1 to cool down the valve seat 1.

[0051] The specific design of the cooling channel is as Figure 3 , Figure 4 , Figure 5 , Figure 8 , Figure 9 As shown in Figure 9 , to realize the full cooling of the valve seat 1, three horizontally penetrating cooling channels are provided inside the valve seat 1, namely the first cooling channel 16, the second cooling channel 17, and the third cooling channel 18.

[0052] Among them, the first cooling channel 16 and the second cooling channel 17 are located at the rear of the valve seat 1 to cool down the rear of the valve seat 1, and they are connected by a vertical channel 163; the first cooling channel 16 is located below, one end is provided with a refrigerant inlet 161 for installing a refrigerant inlet interface 51, and the other end is provided with a first cold plug 162 for installing a plug 6 for sealing; the vertical channel 163 is close to the first cold plug 162, and the top of the vertical channel 163 is provided with a vertical cold plug 1632 for installing a plug 6 for sealing.

[0053] The second cooling channel 17 and the third cooling channel 18 are located at the upper part of the valve seat 1 to cool down the upper part of the valve seat 1, and they are connected by a horizontal channel 173; both ends of the second cooling channel 17 are provided with second cold plugs 172 for installing plugs 6 for sealing; one end of the third cooling channel 18 is provided with a third cold plug 182 for installing a plug 6 for sealing, and the other end is provided with a refrigerant outlet 185 for installing a refrigerant outlet interface 52; the horizontal channel 173 is close to the third cold plug 182; the front end of the horizontal channel 173 is provided with a horizontal cold plug 1732 for installing a plug 6 for sealing.

[0054] The refrigerant is circulating cooling water. After being cooled to 4°C, it is pumped into the cooling channels in the valve seat 1 through the refrigerant inlet interface 51, and flows through the first cooling channel 16, the vertical channel 163, the second cooling channel 17, the horizontal channel 173, and the third cooling channel 18, flowing everywhere in the valve seat 1, especially surrounding the distribution channel 12, the inlet valve channel 13, and the injection channel 14, to cool down the periphery of the medium channel, so that the temperature of the valve seat 1 is lower than 8°C, meeting the usage requirements of low-temperature enzyme reagents. Finally, it is output through the refrigerant outlet interface 52 and then returned to the refrigeration equipment for refrigeration and recycled.

[0055] When the automatic low-temperature valve of the present invention is in use, first start the refrigerant in advance, inject the refrigerant below 4°C into the cooling channels in the valve seat 1, and run for a period of time (generally 5 minutes, which can be adjusted according to the ambient temperature) to reduce the internal temperature of the valve seat 1 from room temperature to below 8°C; then start working, start the pipeline of the low-temperature enzyme reagent, the reagent enters the valve seat 1 through the liquid separation pipeline, and then is controlled by the medium isolation valve 4 and injected into the test tube through the injection steel needle 3; the automatic low-temperature valve of the present invention can be set by a program to automatically add the enzyme reagent, and basically can complete the enzyme addition of a 96-well plate in 48 seconds, with a high automation addition efficiency; at the same time, during the process, the refrigerant continuously circulates and works to cool the valve seat 1, so that the temperature of the internal cavity of the valve island is below 8°C, ensuring the quality of the enzyme reagent in the valve seat 1 and improving the success rate and output efficiency of gene synthesis.

[0056] For the automatic low-temperature valve of the present utility model, the valve seat 1 is first cooled down so that the low-temperature enzyme reagent is always in a low-temperature state, thus reducing the waste of the enzyme reagent and saving costs.

[0057] The preferred embodiments of the present utility model have been specifically described above. However, the present utility model is not limited to the described embodiments. Those skilled in the art can also make various equivalent modifications or substitutions without departing from the spirit of the present utility model. These equivalent modifications or substitutions are all included within the scope defined by the claims of this application.

Claims

1. Automatic low-temperature valve, characterized in that, It includes a valve seat; A liquid inlet interface is installed on one side of the valve seat. A number of liquid injection steel needles are arranged and installed at the bottom of the valve seat. A number of medium isolation valves are arranged and installed at the top of the valve seat. One medium isolation valve corresponds to one liquid injection steel needle; A medium channel is provided inside the valve seat. The liquid inlet interface is connected to the liquid injection steel needle through the medium channel via the medium isolation valve; Cooling interfaces are provided on both sides of the valve seat. A cooling channel is provided inside the valve seat. The cooling channel is isolated from the medium channel; The cooling channel winds around the medium channel; One end of the cooling channel is a refrigerant inlet interface, and the other end is a refrigerant outlet interface; The refrigerant inlet interface and the refrigerant outlet interface are connected to the cooling circulation device through pipelines, and refrigerant flows through the cooling channel.

2. The automated cryogenic valve according to claim 1, wherein, A horizontal liquid inlet flow channel is provided on the front side of the valve seat. The liquid inlet interface is installed at the end of the liquid inlet flow channel. The liquid inlet interface is connected to the reagent barrel through a pipeline; Two columns of channels are provided inside the valve seat in the vertical direction. The front column is a number of inlet valve channels, and the rear column is a number of liquid injection channels; One inlet valve channel and one liquid injection channel form a group and are connected to one medium isolation valve; Mounting screw holes are provided on both sides of a group of inlet valve channels and liquid injection channels for fixing the mounting screws of the medium isolation valve.

3. The automated cryogenic valve according to claim 2, characterized in that, A distribution channel is provided between the liquid inlet flow channel and the inlet valve channel. The distribution channel is horizontally arranged.

4. The automated cryogenic valve according to claim 3, wherein The liquid inlet flow channel is located in the middle of the distribution channel. A number of inlet valve channels are evenly distributed upward from the distribution channel.

5. The automated cryogenic valve according to claim 3, wherein The aperture of the inlet valve channel is smaller than the diameter of the distribution channel.

6. The automated cryogenic valve according to claim 1, wherein, The cooling channel is provided with three horizontally penetrating cooling channels inside the valve seat, namely the first cooling channel, the second cooling channel, and the third cooling channel.

7. The automated cryogenic valve according to claim 6, wherein, The first cooling channel and the second cooling channel are located at the rear of the valve seat. The first cooling channel and the second cooling channel are distributed vertically up and down, and are connected by a vertical channel; The first cooling channel is located below, and one end is provided with a refrigerant inlet and is installed with a refrigerant inlet interface; The vertical channel is far from the refrigerant inlet interface.

8. The automated cryogenic valve according to claim 6 or 7, characterized in that, The second cooling channel and the third cooling channel are located at the upper part of the valve seat. The second cooling channel and the third cooling channel are distributed horizontally front and back, and are connected by a horizontal channel; The third cooling channel is located inside the medium channel. One end of the third cooling channel is provided with a refrigerant outlet and is installed with a refrigerant outlet interface; The horizontal channel is far from the refrigerant outlet interface.

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