Novel in-vitro frog heart perfusion experiment device

Abstract

The utility model discloses a novel in-vitro frog heart perfusion experiment device which comprises a vein cannula and an artery cannula, the top of the vein cannula is communicated with an input end, and the top of the artery cannula is communicated with an output end. The top of the output end is an n-shaped part; the liquid outlet of the output end is communicated with a hose extending into the input end; the output end is fixedly connected with a transverse fixing rod, a sliding ring matched with the transverse fixing rod is fixed to the input end, and the sliding ring is in threaded connection with a first fixing bolt. The transverse fixing rod is connected with a supporting frame. Hooks are fixed to the side faces of the input end and the output end, connecting ropes are fixed to the hooks in a binding mode, and the hooks are used for enhancing binding and fixing of the input end and the output end. The device can effectively prevent an in-vitro frog core from falling off in the experiment process, the distance between the input end and the output end can be adjusted according to the size of the in-vitro frog core, and the left-right position and the high-low position of the whole device can be conveniently adjusted according to needs.

Description

TECHNICAL FIELD

[0001] The utility model relates to the field of biotechnology, specifically relates to a novel isolated frog heart perfusion experimental device. BACKGROUND

[0002] The isolated frog heart is used for the isolated heart perfusion, it can be observed the change of heart activity through perfusing the liquid of different components, for example different drugs, and the same drug of different concentration etc. Because the heart is exposed, therefore can very directly observe the activity change related to heart. Specifically like the application CN 108091231 A discloses a novel isolated frog heart perfusion experimental device and its application method. But the existing perfusion device does not have fixing structure after the sleeve pipe is inserted into the artery and vein of frog heart, and the isolated frog heart is easy to slide in the test process. In addition, because the size of isolated frog heart is different, leading to the interval of the artery and vein of isolated frog heart that need to be inserted into the tube is different, which needs to adjust the interval between the artery sleeve pipe and the vein sleeve pipe. But the existing experimental device has the defects of position fixation, and cannot be adjusted according to the actual size of isolated frog heart, therefore needs improvement. SUMMARY

[0003] The utility model discloses a novel isolated frog heart perfusion experimental device.

[0004] To solve the above problems, the technical scheme of the utility model is:

[0005] A novel isolated frog heart perfusion experimental device, including vein sleeve pipe and artery sleeve pipe, the top of vein sleeve pipe is connected with input end, and the top of artery sleeve pipe is connected with output end, the top of output end is n-shaped part, the output end is connected with the hose that enters into the input end at the liquid outlet, the output end is fixedly connected with the horizontal fixed link, and the input end is fixed with the sliding ring that cooperates with the horizontal fixed link, and the first fixed bolt is screw connected on the sliding ring, the horizontal fixed link is connected with the support frame, and the side of input end and output end is fixed with the hook, and the connecting rope is fixed on the hook and is bound, and is used for strengthening the binding of input end and output end.

[0006] Further improvement, the side of input end is provided with scale.

[0007] Further improvement, the support frame includes base, the vertical rod is fixed on the base, the vertical sleeve is slidably connected on the vertical rod, and the second fixed bolt is screw connected on the vertical sleeve.

[0008] Further improvement, the vertical sleeve is connected with the horizontal sleeve, the horizontal sleeve is connected with the third fixed bolt, and the horizontal fixed link passes through the horizontal sleeve and is fixed with the support frame.

[0009] Further improvement, the top of input end forms arc edge.

[0010] Further improvement, the venous cannula, arterial cannula, input and output are made of transparent glass.

[0011] Further improvement, the hose is a rubber hose.

[0012] Further improvement, the input top is formed with a U-shaped notch.

[0013] Further improvement, the outer periphery of the venous cannula and arterial cannula is formed with a plurality of fixing grooves.

[0014] Further improvement, the hook is on the outer side of the input and output.

[0015] The advantages of the present application are:

[0016] 1. Can effectively prevent the isolated frog heart from falling during the experiment.

[0017] 2. The distance between the input and output can be adjusted according to the size of the isolated frog heart.

[0018] 3. The left and right and height of the whole device can be easily adjusted according to the need.

[0019] 4. The fixed groove can effectively prevent the isolated frog heart from slipping out of the cannula. BRIEF DESCRIPTION OF DRAWINGS

[0020] Figure 1 It is the overall structure schematic diagram of the present application;

[0021] Figure 2 It is the vertical sleeve and the sectional structure schematic diagram of the vertical sleeve. DETAILED DESCRIPTION

[0022] In order to make the above-mentioned purpose, features and advantages of the present application more obvious and easy to understand, the technical scheme in the embodiment of the present application will be described clearly and completely.

[0023] As shown in Figure 1 and Figure 2 A novel isolated frog heart perfusion experiment device, comprising a venous cannula 1, a fixed groove 11, an arterial cannula 2, an input 3, a sliding ring 31, a first fixed bolt 32, a scale 33, an arc-shaped edge 34, a U-shaped notch 35, an output 4, an n-shaped part 41, a hose 42, a horizontal fixing rod 5, a support frame 6, a base 61, a vertical rod 62, a second fixed bolt 63, a horizontal sleeve 64, a third fixed bolt 65, a vertical sleeve 66, a hook 7, a connecting rope 8 and an isolated frog heart 9.

[0024] The hooks are used to strengthen the binding of the input end and the output end, to prevent the isolated frog heart from falling off. The side of the input end 3 and the output end 4 is fixed with a hook 7, and one end of a connecting rope 8 is fixed on the hook 7. After the other end of the connecting rope 8 is inserted into the veins and arteries of the isolated frog heart through the venous cannula 1 and the arterial cannula 2, the connecting rope 8 is wound and fixed with the venous cannula 1 and the arterial cannula 2 respectively, so as to prevent the isolated frog heart from falling off during the experiment. The hook 7 is located on the outer side, which is convenient for operation.

[0025] In order to adjust the distance between the venous cannula 1 and the arterial cannula 2 according to the size of the isolated frog heart, the output end 4 is fixedly connected with a transverse fixing rod 5, and the input end 3 is fixed with a sliding ring 31 matched with the transverse fixing rod 5. The sliding ring 31 is threadedly connected with a first fixing bolt 32. Thus, the position of the input end 3 can be moved according to the need, and the first fixing bolt 32 is tightened after the input end 3 reaches the appropriate position.

[0026] In specific use, the distance between the venous cannula 1 and the arterial cannula 2 is adjusted according to the size of the isolated frog heart, then the venous cannula 1 is inserted into the veins connected with the isolated frog heart, and the arterial cannula 2 is inserted into the arteries connected with the isolated frog heart. The connecting rope 8 is used to fix the veins and the cannula or the arteries and the cannula connected with the isolated frog heart, and then the connecting rope 8 is wound on the hook 7 to prevent the isolated frog heart from falling off. A predetermined amount of liquid medicine is added to the input end 3 according to the scale 33, which is pumped out through the isolated frog heart, and then returns through the n-shaped part 41 of the output end 4. During the process, various indexes of the isolated frog heart can be observed or measured.

[0027] The transverse fixing rod 5 is connected with a support frame 6. The support frame 6 includes a base 61, and a vertical rod 62 is fixed on the base 61. A vertical sleeve 66 is slidably connected to the vertical rod 62, and a second fixing bolt 63 is threadedly connected to the vertical sleeve 66. The vertical sleeve 66 is connected with a horizontal sleeve 64, and a third fixing bolt 65 is connected to the horizontal sleeve 64. The transverse fixing rod 5 passes through the horizontal sleeve 64 and is fixed with the support frame 6. Thus, the entire device can be adjusted left and right or up and down according to the need.

[0028] The U-shaped notch 35 is provided to facilitate the supplement of liquid into the input end 3.

[0029] An arc-shaped edge 34 is formed on the top of the input end 3, which is convenient for pouring liquid medicine. The venous cannula 1, the arterial cannula 2, the input end 3 and the output end 4 are all made of transparent glass, which is convenient for observing the flow of liquid medicine.

[0030] Further, in order to prevent the isolated frog heart from detaching from the cannula, a fixing groove 11 is formed on the outer periphery of the venous cannula 1 and the arterial cannula 2. The connecting rope 8 binds the veins and arteries of the isolated frog heart at the fixing groove 11, thereby effectively preventing the isolated frog heart from slipping off. The fixing groove 11 can be multiple, such as 3-5.

[0031] The above merely describes preferred embodiments of the present application and is not intended to limit the present application, and any modification, equivalent replacement, and improvement within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A novel in vitro frog heart perfusion experimental device, characterized in that, The device includes a venous cannula (1) and an arterial cannula (2). The top of the venous cannula (1) is connected to an input end (3), and the top of the arterial cannula (2) is connected to an output end (4). The top of the output end (4) is an n-shaped part (41). A flexible tube (42) that extends into the input end (3) is connected to the outlet of the output end (4). A transverse fixing rod (5) is fixedly connected to the output end (4). A sliding ring (31) that cooperates with the transverse fixing rod (5) is fixed on the input end (3). A first fixing bolt (32) is threaded onto the sliding ring (31). A support frame (6) is connected to the transverse fixing rod (5). Hooks (7) are fixed on the sides of both the input end (3) and the output end (4). A connecting rope (8) is tied to the hook (7).

2. The novel isolated frog heart perfusion experimental device as described in claim 1, characterized in that, The input terminal (3) has a scale (33) on its side.

3. The novel isolated frog heart perfusion experimental device as described in claim 2, characterized in that, The support frame (6) includes a base (61), a vertical rod (62) is fixed on the base (61), a vertical sleeve (66) is slidably connected on the vertical rod (62), and a second fixing bolt (63) is threadedly connected to the vertical sleeve (66).

4. The novel isolated frog heart perfusion experimental device as described in claim 3, characterized in that, The vertical sleeve (66) is connected to the horizontal sleeve (64), the horizontal sleeve (64) is connected to the third fixing bolt (65), and the horizontal fixing rod (5) passes through the horizontal sleeve (64) and is fixed to the support frame (6).

5. The novel isolated frog heart perfusion experimental device as described in claim 1, characterized in that, The top of the input terminal (3) has an arc-shaped edge (34).

6. The novel isolated frog heart perfusion experimental device as described in claim 1, characterized in that, The venous cannula (1), arterial cannula (2), input end (3), and output end (4) are all made of transparent glass.

7. The novel isolated frog heart perfusion experimental device as described in claim 1, characterized in that, The hose (42) is a rubber hose.

8. The novel isolated frog heart perfusion experimental device as described in claim 1, characterized in that, The top of the input terminal (3) has a U-shaped notch (35).

9. The novel isolated frog heart perfusion experimental device as described in claim 1, characterized in that, Both the venous cannula (1) and the arterial cannula (2) have several fixing grooves (11) formed on their outer periphery.

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