Specimen preparation device for electrophysiological experiment

Abstract

The utility model discloses a specimen preparation device for electrophysiology experiments, which relates to the technical field of specimen preparation and comprises a preparation table, and a timer, a gas flow controller, a temperature controller, a microscope, an electronic balance, a cell separation operation area and a blowing and suction tube accommodating box are arranged on the upper surface of the preparation table. The mixed gas of the oxygen steel cylinder and the carbon dioxide steel cylinder is controlled by the gas flow controller to flow into the first incubation bath tank, the second incubation bath tank and the digestion bath tank, and the gas does not need to be frequently filled from the gas steel cylinders by using an oxygen bag, so that the consumed time is shorter, and the error is smaller; two first rotating shafts are driven by a rotating disc to revolve, then gears are matched with gear rings, so that the two first rotating shafts rotate, second magnets repel or attract first magnets in the revolution process, and the first rotating shafts reciprocate up and down in the revolution process; and the oxygen and the carbon dioxide in the mixing barrel are uniformly mixed.

Description

TECHNICAL FIELD

[0001] The utility model relates to the specimen preparation technical field, concretely is a specimen preparation device for electrophysiological experiment. BACKGROUND

[0002] Electrophysiological experiment is an experimental method for studying bioelectricity and its mechanism, and plays a key role in many fields such as neuroscience, physiology and pharmacology.

[0003] During the experiment, due to the differences in animal anesthesia, the proficiency of the experimenter, and the individual differences of the experimental animals, there are relatively large differences in the morphology and functional state of the obtained nerve tissue, of which hypoxia, freezing damage, poor rewarming, and poor incubation are the main reasons.

[0004] The preparation device used in the previous experiment needs to use an oxygen bag to frequently fill gas from a gas cylinder, which takes a lot of time, has a large error, and affects the experimental results and progress.

[0005] Therefore, the present application provides a specimen preparation device for electrophysiological experiment, which can eliminate the drawbacks of the existing device. SUMMARY

[0006] The utility model discloses a specimen preparation device for electrophysiological experiment, which can solve the problems of the modern product in the background art.

[0007] To achieve the above-mentioned purpose, the utility model provides the following technical scheme:

[0008] A specimen preparation device for electrophysiological experiment, comprising a preparation table, a timer, a gas flow controller, a temperature controller, a microscope, an electronic balance, a separation cell operation area, a blow-suction tube container, a first incubation bath, a second incubation bath, and a digestion bath are arranged on the upper surface of the preparation table, three drawers are arranged on the lower surface of the preparation table, a thermostat is arranged in each of the first incubation bath, the second incubation bath, and the digestion bath, and the thermostat is electrically connected with the temperature controller.

[0009] On the basis of the above-mentioned technical scheme, the utility model also provides the following optional technical scheme:

[0010] In an alternative: the preparation table lower surface is fixedly connected with an oxygen cylinder and a carbon dioxide cylinder through a support, the preparation table upper surface is provided with a mixing barrel, the oxygen cylinder and the mixing barrel are connected with an oxygen connecting pipe, the carbon dioxide cylinder and the mixing barrel are connected with a carbon dioxide connecting pipe, a tee pipe is arranged on the top wall of the mixing barrel, three gas pipes of the tee pipe are respectively inserted into the first incubation bath, the second incubation bath and the digestion bath, the oxygen connecting pipe and the carbon dioxide connecting pipe are both provided with an electric control valve, the electric control valve is electrically connected with a gas flow controller, and the three gas pipes of the tee pipe are all provided with valves.

[0011] In an alternative: the mixing barrel upper surface is fixedly connected with a motor through a support, the mixing barrel top wall is connected with a second rotating shaft through a bearing, the second rotating shaft is fixedly connected with the motor output end, the second rotating shaft lower surface is fixedly connected with a rotating disc, and the rotating disc is provided with two first rotating shafts penetrating therethrough, and the two first rotating shafts are both provided with a plurality of mixing blades.

[0012] In an alternative: the first rotating shaft is fixedly connected with a gear, and the mixing barrel inner side wall is fixedly connected with a gear ring, the gear ring is engaged with the gear.

[0013] In an alternative: the first rotating shaft top end is fixedly connected with a second magnet, the first rotating shaft inner top wall is fixedly connected with a plurality of circumferentially distributed first magnets, adjacent two first magnets are magnetically opposite, and the first rotating shaft is fixedly connected with a limiting plate.

[0014] In an alternative: the first incubation bath, the second incubation bath and the digestion bath are all provided with a filter screen with adjustable depth.

[0015] Compared with the prior art, the utility model has the beneficial effects as follows:

[0016] 1、The utility model discloses a gas flow controller controls the mixed gas of oxygen cylinder and carbon dioxide cylinder to flow into the first incubation bath, the second incubation bath and the digestion bath, and the oxygen bag is not needed to be used to fill the gas from the gas cylinder frequently, and the time consumption is shorter, and the error is smaller.

[0017] 2, the utility model discloses a starting motor, and the motor drives the second rotating shaft to rotate, and the second rotating shaft drives the rotating disc to rotate, and the rotating disc drives two first rotating shafts to revolve, and the gear and the gear ring cooperate when the two first rotating shafts revolve, drive two first rotating shafts to rotate, and at the same time, the first rotating shaft drives the second magnet to revolve, and the second magnet revolving track corresponds the first magnet position, when the second magnet revolves, because the magnetic property of two first magnets adjacent is opposite, so the second magnet and the first magnet repel or attract in the revolving process, make the first rotating shaft reciprocate up and down in the revolving process, and oxygen and carbon dioxide in the mixing barrel are uniformly mixed, and the gas mixing effect is improved. BRIEF DESCRIPTION OF DRAWINGS

[0018] Figure 1 It is the structural schematic diagram of the utility model.

[0019] Figure 2 It is the first perspective drawing of the utility model.

[0020] Figure 3 It is the second perspective drawing of the utility model.

[0021] Figure 4 It is the internal structure schematic diagram of the mixing barrel of the utility model.

[0022] Legend: 1 preparation table, 2 timer, 3 gas flow controller, 4 temperature controller, 5 microscope, 6 electronic balance, 7 separation cell operation area, 8 drawer, 9 blow suction pipe containing box, 10 first incubation bath, 11 second incubation bath, 12 digestion bath, 13 oxygen cylinder, 14 carbon dioxide cylinder, 15 oxygen connecting pipe, 16 carbon dioxide connecting pipe, 17 mixing barrel, 18 filter screen, 19 tee pipe, 20 rotating disc, 21 motor, 22 first magnet, 23 second magnet, 24 gear ring, 25 gear, 26 limit plate, 27 first rotating shaft, 28 second rotating shaft. DETAILED DESCRIPTION

[0023] In order to make the purpose, technical scheme and advantage of the utility model more clearly, the following is in combination with the drawings and example, and the utility model is further detailed.

[0024] In one embodiment, as Figures 1-4As shown, a specimen preparation device for electrophysiological experiment comprises a preparation table 1, a timer 2, a gas flow controller 3, a temperature controller 4, a microscope 5, an electronic balance 6, a separated cell operation area 7, a blow-suction tube containing box 9, a first incubation bath 10, a second incubation bath 11, a digestion bath 12 are arranged on the upper surface of the preparation table 1, three drawers 8 are arranged on the lower surface of the preparation table 1, a thermostat is arranged in each of the first incubation bath 10, the second incubation bath 11 and the digestion bath 12, and the thermostat is electrically connected with the temperature controller 4.

[0025] The first incubation bath 10 and the second incubation bath 11 are pre-filled with tissue incubation solution;

[0026] After the tissue slice is prepared on the oscillating microtome, the tissue slice is moved into the filter screen 18 in the solution in the first incubation bath 10, and the timer 2 is used to incubate for 1 hour;

[0027] After the incubation, an appropriate amount and concentration of enzyme digestion solution is added into the digestion bath 12, the enzyme is weighed by the electronic balance 6, mixed gas is introduced into the digestion bath 12, 2-3 tissue slices are selected from the first incubation bath 10 and are transferred to the filter screen 18 in the digestion bath 12, and the digestion is performed for about 0.5 hours, and the digestion time can be adjusted according to the age of the animal;

[0028] After the enzyme digestion is completed, the tissue slice in the digestion bath 12 is transferred to the filter screen 18 in the second incubation bath 11, and is incubated for about 1 hour, so that the processing of the electrophysiological experimental specimen is completed.

[0029] The specific part of the tissue slice can be pre-cut and separated. Under the assistance of the separated cell operation area 7 and the microscope 5, single cells are mechanically separated from the tissue slice after enzyme digestion by means of blow-suction tubes with different diameters for standby use.

[0030] In an embodiment, the lower surface of the preparation table 1 is fixedly connected with an oxygen cylinder 13 and a carbon dioxide cylinder 14 through a support, a mixing barrel 17 is arranged on the upper surface of the preparation table 1, an oxygen connecting pipe 15 is connected between the oxygen cylinder 13 and the mixing barrel 17, a carbon dioxide connecting pipe 16 is connected between the carbon dioxide cylinder 14 and the mixing barrel 17, a tee pipe 19 is arranged on the top wall of the mixing barrel 17, three gas conveying pipes of the tee pipe 19 are respectively inserted into the first incubation bath 10, the second incubation bath 11 and the digestion bath 12, the oxygen connecting pipe 15 and the carbon dioxide connecting pipe 16 are both provided with electric control valves, the electric control valves are electrically connected with the gas flow controller 3, and valves are arranged on the three gas conveying pipes of the tee pipe 19.

[0031] The oxygen cylinder 13 and the carbon dioxide cylinder 14 are controlled by the gas flow controller 3 to continuously introduce the mixed gas of 95% oxygen + 5% carbon dioxide into the first incubation bath 10 and the second incubation bath 11, and the temperature in the first incubation bath 10 and the second incubation bath 11 is controlled by the temperature controller 4 to be room temperature.

[0032] In one embodiment, the upper surface of the mixing barrel 17 is fixedly connected with the motor 21 through a support, the top wall of the mixing barrel 17 is penetratively connected with the second rotating shaft 28 through a bearing, the second rotating shaft 28 is fixedly connected with the output end of the motor 21, the lower surface of the second rotating shaft 28 is fixedly connected with the rotating disc 20, and the rotating disc 20 is penetratively provided with two first rotating shafts 27, and each of the two first rotating shafts 27 is provided with a plurality of mixing blades.

[0033] The motor 21 is started, the motor 21 drives the second rotating shaft 28 to rotate, the second rotating shaft 28 drives the rotating disc 20 to rotate, and the rotating disc 20 drives the two first rotating shafts 27 to revolve to uniformly mix the gas.

[0034] In one embodiment, the first rotating shaft 27 is fixedly connected with the gear 25 on the side, and the inner side wall of the mixing barrel 17 is fixedly connected with the gear ring 24, and the gear ring 24 is engaged with the gear 25.

[0035] The two first rotating shafts 27 revolve while the gear 25 cooperates with the gear ring 24 to drive the two first rotating shafts 27 to rotate, thereby uniformly mixing the oxygen and the carbon dioxide in the mixing barrel 17.

[0036] In one embodiment, the first rotating shaft 27 is fixedly connected with the second magnet 23 at the top end, the inner top wall of the first rotating shaft 27 is fixedly connected with a plurality of circumferentially distributed first magnets 22, the magnetic properties of adjacent two first magnets 22 are opposite, and the first rotating shaft 27 is fixedly connected with the limiting plate 26 on the side.

[0037] At the same time, in the revolving process of the first rotating shaft 27 driving the second magnet 23 to revolve, the revolving track of the second magnet 23 corresponds to the position of the first magnet 22, and when the second magnet 23 revolves, since the magnetic properties of adjacent two first magnets 22 are opposite, the second magnet 23 repels or attracts the first magnet 22 in the revolving process, so that the first rotating shaft 27 reciprocates up and down in the revolving process, thereby further improving the mixing effect of the gas.

[0038] In one embodiment, the first incubation bath 10, the second incubation bath 11 and the digestion bath 12 are all provided with the filter screen 18 with adjustable depth.

[0039] The slice is placed on the filter screen 18 to make the slice fully contact with oxygen and keep active.

[0040] The above embodiment discloses a specimen preparation device for electrophysiological experiment, and the specific working principle and process are as follows:

[0041] S1: The tissue incubation solution is added in advance into the first incubation bath 10 and the second incubation bath 11.

[0042] S2: The oxygen cylinder 13 and the carbon dioxide cylinder 14 are controlled by the gas flow controller 3, and the mixed gas of 95% oxygen and 5% carbon dioxide is continuously introduced into the first incubation bath 10 and the second incubation bath 11, and the temperature in the first incubation bath 10 and the second incubation bath 11 is controlled to room temperature by the temperature controller 4.

[0043] S3: After the tissue section is completed on the oscillating microtome, the tissue section is moved into the filter screen 18 in the solution in the first incubation bath 10, and the timer 2 is used for timed incubation for 1 hour, and the mixed gas is continuously mixed.

[0044] S4: After incubation, the appropriate amount and concentration of enzyme digestion solution is added in the digestion bath 12, the enzyme is weighed by the electronic balance, the mixed gas is introduced into the digestion bath 12, 2-3 tissue sections are selected from the first incubation bath 10 and transferred to the filter screen 18 in the digestion bath 12, and the digestion time is about 0.5 hours, which can be adjusted according to the age of the animal.

[0045] S5: After the enzyme digestion is completed, the tissue section in the digestion bath 12 is transferred to the filter screen 18 in the second incubation bath 11, and incubated for about 1 hour, and the processing of the electrophysiological experiment specimen is completed.

[0046] For the specific part of the tissue section, the specific part can be cut and separated in advance, and under the assistance of the separation cell operation area 7 and the microscope 5, the single cell is separated from the tissue section by means of the blow and suction pipe with different diameters for standby.

[0047] When the gas mixing is needed, the motor 21 is started, the second rotating shaft 28 is rotated by the motor 21, the rotating disc 20 is rotated by the second rotating shaft 28, the two first rotating shafts 27 are revolved by the rotating disc 20, the two first rotating shafts 27 revolve while the gear 25 cooperates with the gear ring 24 to drive the two first rotating shafts 27 to rotate, and the oxygen and the carbon dioxide in the mixing barrel 17 are uniformly mixed, and at the same time, the second magnet 23 revolves in the process of revolving driven by the first rotating shaft 27, the revolving track of the second magnet 23 corresponds to the position of the first magnet 22, and when the second magnet 23 revolves, the second magnet 23 repels or attracts the first magnet 22 in the revolving process due to the opposite magnetic properties of the adjacent two first magnets 22, so that the first rotating shaft 27 moves up and down in the revolving process, and the mixing effect of the gas is further improved.

[0048] The above merely provides the specific implementation of the present application, but the protection scope of the present application is not limited to this. Any person skilled in the art can easily think of the changes or replacements within the technical range disclosed by the present application, which should be covered in the protection scope of the present application. Therefore, the protection scope of the present application should be subject to the protection scope of the claims.

Claims

1. A specimen preparation apparatus for electrophysiological experiments, characterized in that, The preparation table (1) includes a timer (2), a gas flow controller (3), a temperature controller (4), a microscope (5), an electronic balance (6), a cell separation operation area (7), a blowpipe container (9), a first incubation bath (10), a second incubation bath (11), and a digestion bath (12) on its upper surface. The preparation table (1) has three drawers (8) on its lower surface. Each of the first incubation bath (10), the second incubation bath (11), and the digestion bath (12) is equipped with a thermostat, which is electrically connected to the temperature controller (4).

2. The specimen preparation device for electrophysiological experiments according to claim 1, characterized in that, The lower surface of the preparation table (1) is fixedly connected to an oxygen cylinder (13) and a carbon dioxide cylinder (14) by a bracket. A mixing tank (17) is provided on the upper surface of the preparation table (1). An oxygen connecting pipe (15) is connected between the oxygen cylinder (13) and the mixing tank (17). A carbon dioxide connecting pipe (16) is connected between the carbon dioxide cylinder (14) and the mixing tank (17). A three-way pipe (19) is provided on the top wall of the mixing tank (17). The three gas supply pipes of the three-way pipe (19) are respectively inserted into the first incubation bath (10), the second incubation bath (11), and the digestion bath (12). Both the oxygen connecting pipe (15) and the carbon dioxide connecting pipe (16) are provided with electrically controlled valves. The electrically controlled valves are electrically connected to the gas flow controller (3). Valves are provided on the three gas supply pipes of the three-way pipe (19).

3. The specimen preparation device for electrophysiological experiments according to claim 2, characterized in that, A motor (21) is fixedly connected to the upper surface of the mixing tank (17) by a bracket. A second rotating shaft (28) is connected through the top wall of the mixing tank (17) by a bearing. The second rotating shaft (28) is fixedly connected to the output end of the motor (21). A rotating disk (20) is fixedly connected to the lower surface of the second rotating shaft (28). Two first rotating shafts (27) are provided through the rotating disk (20). Each of the two first rotating shafts (27) is provided with several mixing blades.

4. The specimen preparation device for electrophysiological experiments according to claim 3, characterized in that, A gear (25) is fixedly connected to the circumference of the first rotating shaft (27), and a gear ring (24) is fixedly connected to the inner wall of the mixing barrel (17), and the gear ring (24) meshes with the gear (25).

5. The specimen preparation device for electrophysiological experiments according to claim 4, characterized in that, A second magnet (23) is fixedly connected to the top of the first rotating shaft (27), and a number of circumferentially distributed first magnets (22) are fixedly connected to the inner top wall of the first rotating shaft (27). The magnetic properties of two adjacent first magnets (22) are opposite, and a limit plate (26) is fixedly connected to the periphery of the first rotating shaft (27).

6. The specimen preparation apparatus for electrophysiological experiments according to claim 1, characterized in that, The first incubation bath (10), the second incubation bath (11), and the digestion bath (12) are all equipped with adjustable-depth filters (18).

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