Primary cell extraction device

Abstract

The utility model discloses a primary cell extraction device, which relates to the technical field of cell extraction, and comprises a cell centrifugal machine and a collecting device, the bottom of the cell centrifugal machine is fixedly connected with the top of the collecting device, in the utility model, the device can automatically cut and crush a cell sample through the reverse rotation of a left group of cutters and a right group of cutters; manual intervention is greatly reduced, the cell extraction efficiency is improved, meanwhile, the crushing consistency of a packaged sample can be achieved, the purity and yield of subsequent cell extraction are improved, a cell centrifugal machine is started according to a preset separation program for primary cells, the centrifugal process is automatically completed, layering of the cells and impurities is achieved, and after centrifugation is finished, the cell extraction efficiency is improved. Separated cells and supernate can be accurately collected into corresponding containers through the collection device, tissue smashing, cell separation and collection are integrated in one device, the sample transfer frequency is reduced, the pollution risk is reduced, and the working efficiency is improved.

Description

Technical Field

[0001] This utility model relates to the field of cell extraction technology, and in particular to a primary cell extraction device. Background Technology

[0002] Primary cells are cells that are cultured immediately after being removed from the body. They have crucial applications in many fields such as biological research, drug development, and regenerative medicine. Primary cells have a short in vitro culture time and their genetic traits are similar to those of cells in vivo, making them suitable for studying cell morphology, function, and differentiation. Primary isolation and extraction refers to the process of separating a single-cell suspension from tissues in vivo. Generally, animal tissues are surgically removed, the tissues are artificially cut into pieces, and then the tissues are digested and filtered to obtain primary cells. These primary cells are then cultured in vitro for cell experiments.

[0003] For example, Chinese patent CN214115521U discloses an experimental device for extracting primary cells from solid tissues, including a sleeve, a cap, a stirring cap, and a filter cylinder. The cap can cover the top opening of the sealed sleeve; the stirring cap includes a cap body, a stirring shaft, and a handle; the cap body can cover the top opening of the sealed sleeve, and a rotary motor is installed inside the cap body; the top of the stirring shaft is detachably fixedly connected to the shaft of the rotary motor, and stirring blades are provided on the stirring shaft; the handle is integrated with the cap body, and a power supply is provided inside the handle; the filter cylinder is detachably installed on the upper inner side of the sleeve.

[0004] Currently, primary cell extraction typically relies on manual operation, using tissue scissors to cut and pulverize tissue samples. This method is not only inefficient, but also difficult to guarantee operational consistency. Inconsistent tissue fragmentation can easily occur due to differences in operator technique, which in turn affects the purity and yield of subsequent cell extraction. To address these issues, a primary cell extraction device is proposed. Utility Model Content

[0005] The purpose of this invention is to solve the problems existing in the prior art by proposing a primary cell extraction device.

[0006] To achieve the above objectives, the present invention adopts the following technical solution: a primary cell extraction device, comprising a cell centrifuge and a collection device, wherein the bottom of the cell centrifuge is fixedly connected to the top of the collection device, and a pulverizing device is fixedly connected to the top of the cell centrifuge. The pulverizing device includes a controller, a pulverizing cylinder, a discharge pipe, a motor, a rotating wheel, and a rotating rod. The output end of the motor is fixedly connected to one end of the rotating wheel. Two bevel gears are symmetrically fixedly connected to both ends of the rotating wheel. Two bevel gears are symmetrically rotatably connected to the top wall of the pulverizing cylinder. The outer wall of the bevel gears meshes with the outer side of the bevel gears, and the bottom end of the bevel gears is fixedly installed to the top of the rotating rod. Multiple cutting blades are fixedly connected in a circular array to the outer side of the top of the rotating rod, and multiple pulverizing blades are fixedly connected to the outer side of the rotating rod. A feeding pipe is fixedly connected to the top of the pulverizing cylinder. The top of the discharge pipe is fixedly connected to the bottom of the pulverizing cylinder, and a solenoid valve is fixedly connected to the bottom of the discharge pipe. A filter screen is fixedly connected to the inner wall of the top of the discharge pipe.

[0007] Preferably, a liquid addition pipe is fixedly connected to the top of the grinding cylinder, and a liquid discharge pipe is fixedly connected to the bottom of the grinding cylinder.

[0008] Preferably, a valve is fixedly connected to one end of the drain pipe, and a protective cover is fixedly installed on the top of the pulverizing cylinder.

[0009] Preferably, one end of the protective cover is fixedly installed with one end of the controller, and the bottom of the discharge pipe is fixedly connected to the top of the cell centrifuge.

[0010] Preferably, the collection device includes a base, a diversion tube, a connecting tube, and two collection containers. The top of the connecting tube is fixedly connected to the bottom of the cell centrifuge, and the bottom of the connecting tube is fixedly connected to the top of the diversion tube.

[0011] Preferably, two diversion valves are symmetrically and fixedly connected to both ends of the diversion pipe, and a collection pipe is fixedly connected to one end of each diversion valve.

[0012] Preferably, the base has symmetrical notches on both sides, and the top of the base is fixedly installed on the outer side of the bottom of the cell centrifuge.

[0013] Compared with the prior art, the advantages and positive effects of this utility model are as follows:

[0014] 1. In this utility model, a motor drives a rotating wheel and two bevel gears at both ends to rotate, which in turn drives two bevel gears and a rotating rod to rotate. The two bevel gears rotate in opposite directions, which in turn causes multiple pulverizing blades and multiple cutting blades on the outside of the rotating rod to rotate. The multiple pulverizing blades and multiple cutting blades perform fine cutting of the tissue inside the pulverizing cylinder. This device can automatically cut and pulverize cell samples by rotating the left and right sets of blades in opposite directions, which greatly reduces manual intervention, improves cell extraction efficiency, and can also ensure the consistency of pulverized packaged samples, thereby improving the purity and yield of subsequent cell extraction.

[0015] 2. In this invention, the cell centrifuge is started according to a preset separation program for primary cells, and the centrifugation process is automatically completed, achieving the separation of cells and impurities. After centrifugation, the separated cells and supernatant can be accurately collected into corresponding containers by the collection device, completing the extraction and preliminary classification of primary cells. The whole process is simple and efficient. This device integrates tissue pulverization, cell separation and collection into one device, reducing the number of sample transfers, reducing the risk of contamination and improving work efficiency. Attached Figure Description

[0016] Figure 1 This is a three-dimensional structural diagram of a primary cell extraction device proposed in this utility model.

[0017] Figure 2 This is a top view of a primary cell extraction device proposed in this utility model;

[0018] Figure 3 This is a front sectional view of a primary cell extraction device proposed in this utility model;

[0019] Figure 4 This is a top view of the internal structure of the pulverizing cylinder of a primary cell extraction device proposed in this utility model. Legend: 1. Pulverizing device; 2. Cell centrifuge; 3. Collection device; 11. Controller; 12. Protective cover; 13. Pulverizing cylinder; 14. Feeding pipe; 15. Liquid adding pipe; 16. Drain pipe; 17. Valve; 18. Discharge pipe; 19. Solenoid valve; 110. Motor; 111. Rotating wheel; 112. Bevel gear one; 113. Bevel gear two; 114. Rotating rod; 115. Pulverizing blade; 116. Cutting blade; 117. Filter screen; 31. Base; 32. Notch; 33. Collection container; 34. Diverter pipe; 35. Diverter valve; 36. Collection pipe; 37. Connecting pipe. Detailed Implementation

[0020] To better understand the above-mentioned objectives, features, and advantages of this utility model, the present utility model will be further described below with reference to the accompanying drawings and embodiments. It should be noted that, unless otherwise specified, the embodiments and features described in these embodiments can be combined with each other.

[0021] Many specific details are set forth in the following description in order to provide a full understanding of the present invention. However, the present invention may also be implemented in other ways different from those described herein. Therefore, the present invention is not limited to the specific embodiments disclosed in the following specification.

[0022] Example 1: As Figure 1 - Figure 4 As shown, this utility model provides a primary cell extraction device, including a cell centrifuge 2 and a collection device 3. The bottom of the cell centrifuge 2 is fixedly connected to the top of the collection device 3. A pulverizing device 1 is fixedly connected to the top of the cell centrifuge 2. The pulverizing device 1 includes a controller 11, a pulverizing cylinder 13, a discharge pipe 18, a motor 110, a rotating wheel 111, and a rotating rod 114. The output end of the motor 110 is fixedly connected to one end of the rotating wheel 111. Two bevel gears 112 are symmetrically fixedly connected to both ends of the rotating wheel 111. Two bevel gears 113 are symmetrically rotatably connected to the top wall of the pulverizing cylinder 13. The outer wall of the bevel gears 113 meshes with the outer side of the bevel gears 112, and the bottom end of the bevel gears 113 is fixedly installed to the top of the rotating rod 114. Multiple cutting blades 116 are fixedly connected to the outer ring of the top of the 14, and multiple crushing blades 115 are fixedly connected to the outer side of the rotating rod 114. The top of the crushing cylinder 13 is fixedly connected to the feeding pipe 14. The top of the discharge pipe 18 is fixedly connected to the bottom of the crushing cylinder 13, and the bottom of the discharge pipe 18 is fixedly connected to the solenoid valve 19. The inner wall of the top of the discharge pipe 18 is fixedly connected to the filter screen 117. The top of the crushing cylinder 13 is fixedly connected to the liquid feeding pipe 15, and the bottom of the crushing cylinder 13 is fixedly connected to the drain pipe 16. One end of the drain pipe 16 is fixedly connected to the valve 17. The top of the crushing cylinder 13 is fixedly installed with a protective cover 12. One end of the protective cover 12 is fixedly installed with one end of the controller 11. The bottom of the discharge pipe 18 is fixedly connected to the top of the cell centrifuge 2.

[0023] The specific setup and function of this embodiment are described below: The device drives the rotating wheel 111 and the two bevel gears 112 at both ends to rotate via the motor 110, thereby driving the two bevel gears 113 and the rotating rod 114 to rotate. The two bevel gears 113 rotate in opposite directions, which in turn causes the multiple pulverizing blades 115 and multiple cutting blades 116 on the outside of the rotating rod 114 to rotate. The multiple pulverizing blades 115 and multiple cutting blades 116 perform fine cutting on the tissue inside the pulverizing cylinder 13. Through the opposite rotation of the left and right sets of blades, the cell sample can be automatically cut and pulverized, greatly reducing manual intervention and improving cell extraction efficiency. At the same time, it can also ensure the consistency of the packaged sample pulverization, improve the purity and yield of subsequent cell extraction. In addition, this device integrates tissue pulverization, cell separation and collection into one device, reducing the number of sample transfers, reducing the risk of contamination, and shortening the overall operation time, thereby improving work efficiency.

[0024] Example 2: Figure 1 and Figure 3 As shown, the collection device 3 includes a base 31, a diversion tube 34, a connecting tube 37, and two collection containers 33. The top of the connecting tube 37 is fixedly connected to the bottom of the cell centrifuge 2, and the bottom of the connecting tube 37 is fixedly connected to the top of the diversion tube 34. Two diversion valves 35 are symmetrically fixedly connected to both ends of the diversion tube 34, and a collection tube 36 is fixedly connected to one end of the diversion valve 35. Notches 32 are symmetrically opened on both sides of the base 31, and the top of the base 31 is fixedly installed on the outer side of the bottom end of the cell centrifuge 2.

[0025] The overall effect of this embodiment is that after centrifugation, one of the diversion valves 35 is opened by the controller 11 to introduce the lower cell pellet into the collection container 33 through the connecting tube 37, diversion tube 34 and collection tube 36. Then, the other diversion valve 35 is opened by the controller 11 to introduce the upper supernatant into another collection container 33 through the connecting tube 37, diversion tube 34 and collection tube 36. This allows for the precise collection of the separated cells and supernatant into their respective containers. The collection container 33 is placed in the base 31 and kept at a low temperature, thus completing the extraction and preliminary classification of primary cells. The entire process is simple and efficient.

[0026] The device's operation and working principle are as follows: This primary cell extraction device mainly consists of a pulverizer 1, a cell centrifuge 2, and a collection device 3. When cell extraction is required from a tissue sample, the first step is cell processing. The fresh tissue sample is placed into the pulverizer 13 through the feeding tube 14. The feeding tube 14 is then closed, and the motor 110 is started via the controller 11. The motor 110 drives the rotating wheel 111 and the two bevel gears 112 at both ends to rotate. The outer side of the bevel gear 112 meshes with the outer side of the bevel gear 113, thereby driving the two bevel gears 113 and the rotating rod 114 to rotate. The two bevel gears 113 rotate in opposite directions, thus causing multiple pulverizers on the outer side of the rotating rod 114 to rotate. The blade 115 and multiple cutting blades 116 rotate, and the multiple pulverizing blades 115 and multiple cutting blades 116 finely cut the tissue in the pulverizing cylinder 13. After the cutting is completed, the motor 110 is turned off and the solenoid valve 19 is opened. The filter screen 117 filters and screens the pulverized sample fragments. The sample fragments that meet the pulverization requirements flow into the cell centrifuge 2 through the filter screen 117 and the discharge pipe 18, thus entering the cell centrifugation stage. This device can automatically cut and pulverize cell samples by rotating the left and right sets of blades in opposite directions, which greatly reduces manual intervention, improves cell extraction efficiency, and can also ensure the consistency of packaged sample pulverization, thereby improving the purity and yield of subsequent cell extraction.

[0027] The cell centrifuge 2 starts according to the preset separation program for primary cells and automatically completes the centrifugation process, achieving stratification of cells and impurities. After centrifugation, the controller 11 opens one of the diversion valves 35 to introduce the lower cell precipitate into the collection container 33 through the connecting tube 37, diversion tube 34 and collection tube 36. Then, the controller 11 opens another diversion valve 35 to introduce the upper supernatant into another collection container 33 through the connecting tube 37, diversion tube 34 and collection tube 36. This can accurately collect the separated cells and supernatant into the corresponding containers. The collection container 33 is placed in the base 31 and kept at a low temperature, completing the extraction and preliminary classification of primary cells. The whole process is simple and efficient.

[0028] Finally, open the liquid inlet pipe 15 and inject cleaning solution into the pulverizing cylinder 13. Turn on the motor 110 to rinse the inner wall of the pulverizing cylinder 13 and the blades (multiple pulverizing blades 115 and multiple cutting blades 116). After rinsing, open the valve 17 and discharge the waste liquid through the drain pipe 16. This device integrates tissue pulverization, cell separation and collection into one device, reducing the number of sample transfers, reducing the risk of contamination, and shortening the overall operation time and improving work efficiency.

[0029] The above description is merely a preferred embodiment of the present utility model and is not intended to limit the present utility model in any other way. Any person skilled in the art may make changes or modifications to the above-disclosed technical content to create equivalent embodiments for application in other fields. However, any simple modifications, equivalent changes, and modifications made to the above embodiments based on the technical essence of the present utility model without departing from the technical solution of the present utility model shall still fall within the protection scope of the present utility model.

Claims

1. A primary cell extraction device, comprising a cell centrifuge (2) and a collection device (3), wherein the bottom of the cell centrifuge (2) is fixedly connected to the top of the collection device (3), characterized in that: A pulverizing device (1) is fixedly connected to the top of the cell centrifuge (2). The pulverizing device (1) includes a controller (11), a pulverizing cylinder (13), a discharge pipe (18), a motor (110), a rotating wheel (111), and a rotating rod (114). The output end of the motor (110) is fixedly connected to one end of the rotating wheel (111). Two bevel gears (112) are symmetrically fixedly connected to both ends of the rotating wheel (111). Two bevel gears (113) are symmetrically rotatably connected to the top wall of the pulverizing cylinder (13). The outer wall of the bevel gears (113) is connected to the outer side of the bevel gears (112). The bevel gear (113) is meshed with the bottom end of the rotating rod (114), and the rotating rod (114) is fixedly connected to the outer side of the top end of the rotating rod (114) in a ring array. The rotating rod (114) is also fixedly connected to the outer side of the rotating rod (114) in a ring array. The feeding pipe (14) is fixedly connected to the top end of the crushing cylinder (13). The top end of the discharge pipe (18) is fixedly connected to the bottom end of the crushing cylinder (13), and the bottom end of the discharge pipe (18) is fixedly connected to a solenoid valve (19). The inner wall of the top end of the discharge pipe (18) is fixedly connected to a filter screen (117).

2. The primary cell extraction device according to claim 1, characterized in that: The top end of the pulverizing cylinder (13) is fixedly connected to a liquid adding pipe (15), and the bottom end of the pulverizing cylinder (13) is fixedly connected to a liquid draining pipe (16).

3. The primary cell extraction device according to claim 2, characterized in that: A valve (17) is fixedly connected to one end of the drain pipe (16), and a protective cover (12) is fixedly installed on the top of the crushing cylinder (13).

4. The primary cell extraction device according to claim 3, characterized in that: One end of the protective cover (12) is fixedly installed with one end of the controller (11), and the bottom of the discharge pipe (18) is fixedly connected to the top of the cell centrifuge (2).

5. The primary cell extraction device according to claim 1, characterized in that: The collection device (3) includes a base (31), a diversion tube (34), a connecting tube (37), and two collection containers (33). The top of the connecting tube (37) is fixedly connected to the bottom of the cell centrifuge (2), and the bottom of the connecting tube (37) is fixedly connected to the top of the diversion tube (34).

6. The primary cell extraction apparatus according to claim 5, characterized in that: Two diversion valves (35) are symmetrically fixedly connected to both ends of the diversion pipe (34), and a collection pipe (36) is fixedly connected to one end of each diversion valve (35).

7. The primary cell extraction apparatus according to claim 5, characterized in that: The base (31) has symmetrical notches (32) on both sides, and the top of the base (31) is fixedly installed on the outer side of the bottom of the cell centrifuge (2).

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