Fluorescent dye molecule detection equipment

Abstract

The utility model relates to the technical field of fluorescent dye molecule detection, in particular to fluorescent dye molecule detection equipment. The device comprises a workbench, the top of the workbench is fixedly connected with a detection box, a light source and a detection probe are arranged in the top end of the detection box, and a bearing plate is arranged in the bottom end of the detection box; a containing groove is formed in the top of the bearing plate in an embedded mode. A mounting frame is fixedly connected to the bottom of the bearing plate, a first motor is fixedly connected to the top of the mounting frame, an output shaft of the first motor is fixedly connected to the bottom of the containing groove, and the output shaft of the first motor is rotationally connected to the bearing plate through a bearing; by driving the placing groove, sample particles can be redistributed, the uniformity of the fluorescent dye can be improved, the overall fluorescence condition of the sample can be reflected more truly, and fluorescent pigment molecules in each layer from the surface to the inside can be effectively excited through rotation.

Description

Technical Field

[0001] This utility model relates to the field of fluorescent dye molecule detection technology, and in particular to a fluorescent dye molecule detection device. Background Technology

[0002] Fluorescent dyes are substances that, after absorbing a certain wavelength of light, can emit another wavelength of light with a longer wavelength than the absorbed light. Most of them are compounds containing benzene rings or heterocycles and conjugated double bonds. Fluorescent dyes can be used alone or in combination into composite fluorescent dyes. Fluorescent dye molecules need to be detected by detection equipment before use.

[0003] A Chinese patent with publication number CN207730668U discloses a fluorescent dye molecule detection device, including a worktable, a device body, a detection and processing chamber, a fluorescent dye receiving plate, and a magnetic groove. The device body is fixed at the center of the top of the worktable, and the detection and processing chamber is located at the center of the inside of the device body. The fluorescent dye receiving plate is installed on the two side walls of the detection and processing chamber via sliding strips, and a fluorescent dye placement chamber is located at the center of the top of the fluorescent dye receiving plate. A detector cover is hinged to the center of the device body above the fluorescent dye receiving plate, and a molecular detector is fixed on the inner side wall of the detector cover. A control panel is installed on the top of the device body on one side of the molecular detector. By installing a fixing plate at the center of the inner side wall of the placement chamber cover, and installing a height adjustment column at the end of the fixing plate away from the inner side wall of the placement chamber cover, and by fixing a support frame at the bottom of the height adjustment column, the height and angle adjustment function of the laser emission source is realized.

[0004] In current technologies, fluorescent dyes are placed in a fluorescent dye placement cavity during detection. However, the fluorescent dye placement cavity is a common groove. If the dye is liquid, it can flow freely and make the dye level. If the dye is solid, uneven stacking may prevent some fluorescent dye molecules from being effectively excited, leading to deviations in fluorescence detection.

[0005] Therefore, a fluorescent dye molecule detection device is proposed to address the above problems. Utility Model Content

[0006] Therefore, the technical problem to be solved by this invention is to overcome the fact that some fluorescent dye molecules cannot be effectively excited due to uneven stacking in the prior art.

[0007] To solve the above-mentioned technical problems, this utility model provides a fluorescent dye molecule detection device.

[0008] In one embodiment of this utility model, a workbench is included. A detection box is fixedly connected to the top of the workbench. A light source and a detection probe are provided inside the top of the detection box, and a receiving plate is provided inside the bottom of the detection box. A placement groove is embedded in the top of the receiving plate. A mounting frame is fixedly connected to the bottom of the receiving plate, and a first motor is fixedly connected to the top of the mounting frame. The output shaft of the first motor is fixedly connected to the bottom of the placement groove, and the output shaft of the first motor is rotatably connected to the receiving plate through a bearing. Slide rods are fixedly connected to both ends of the receiving plate, and slide rails are symmetrically fixed to the inner walls of both sides of the detection box. The slide rods are slidably connected within the slide rails. Multiple anti-slip grooves are provided at one end of the receiving plate.

[0009] In one embodiment of this utility model, a second motor is fixedly connected to the top of the detection box, the output shaft of the second motor is rotatably connected to the detection box, and a rotating frame is fixedly connected thereto. The second motor is located at one end of the rotating frame. A reciprocating lead screw is rotatably connected to the rotating frame via a bearing. A moving block is threaded onto the reciprocating lead screw. A second side plate is fixedly connected to one side wall of the moving block. A second electric push rod is fixedly connected to the bottom of the second side plate. A light source is installed on the output end of the second electric push rod. A third motor is provided at one end of the reciprocating lead screw. The rotating frame is longer than the radius of the placement slot.

[0010] In one embodiment of the present invention, a first side plate is fixedly connected to the other side wall of the movable block, a first electric push rod is fixedly connected to the bottom of the first side plate, and a detection probe is installed on the output end of the first electric push rod.

[0011] In one embodiment of this utility model, a temperature sensor is installed on the top inner wall of the detection box, and a dustproof plate is installed on the detection box with a perforation; a first fan is installed on the inner wall of the dustproof plate; an electric heating wire is provided on the inner side of the first fan, and the electric heating wire is electrically connected to the temperature sensor.

[0012] In one embodiment of this utility model, a mounting frame is fixedly connected to the top of the detection box, and a second fan is installed inside the mounting frame. The second fan is electrically connected to the temperature sensor.

[0013] In one embodiment of this utility model, a sealing plate is slidably connected to the side wall of the detection box away from the first fan, and a handle is fixedly connected to the outer wall of the sealing plate; a first magnetic block is fixedly connected to the side wall of the detection box, and the first magnetic block is in contact with the top of the handle; a second magnetic block is embedded and fixedly connected to the top of the workbench, and the second magnetic block is in contact with the bottom of the handle.

[0014] In one embodiment of this utility model, a controller and a transparent window are installed on the side wall of the detection box, and the controller is electrically connected to the detection probe.

[0015] In one embodiment of this utility model, two guide rods are fixedly connected to the rotating frame, and the moving block is slidably connected to the two guide rods.

[0016] The above-mentioned technical solution of this utility model has the following advantages compared with the prior art:

[0017] The fluorescent dye molecule detection device described in this utility model can redistribute sample particles by driving the placement groove, which can improve the uniformity of fluorescent dye and more accurately reflect the overall fluorescence of the sample. The rotation can ensure that fluorescent pigment molecules at all levels from the surface to the interior can be effectively excited.

[0018] The fluorescent dye molecule detection device described in this utility model features a reciprocating screw and a rotating frame. The reciprocating screw facilitates adjustment of the position of the light source and the detection probe, while the rotating frame facilitates rotation of the light source and the detection probe, thereby increasing the detection range. After the fluorescent dye is irradiated by the light source, the detection probe is activated to begin detection. The excitation light source emits excitation light according to the set parameters, which is transmitted through the optical path and irradiates the fluorescent dye. The fluorescence signal generated by the fluorescent dye is received by the detection probe and converted into an electrical signal. The data is then transmitted to the data processing system for data analysis. Attached Figure Description

[0019] To make the content of this utility model easier to understand, the present utility model will be further described in detail below with reference to specific embodiments and accompanying drawings.

[0020] Figure 1 This is the first perspective view of the present invention;

[0021] Figure 2 This is the second perspective view of the present invention;

[0022] Figure 3 This is a sectional view of the present invention;

[0023] Figure 4 This is a perspective view of the receiving plate in this utility model;

[0024] Figure 5 This is a perspective view of the movable block in this utility model;

[0025] Explanation of reference numerals in the accompanying drawings: 1. Workbench; 2. Detection box; 3. Sealing plate; 4. Slide rail; 5. Slide rod; 6. Receiving plate; 7. Placement slot; 8. First motor; 9. Mounting bracket; 10. Second motor; 11. Rotating bracket; 12. Reciprocating lead screw; 13. Guide rod; 14. Third motor; 15. Moving block; 16. First side plate; 17. First electric actuator; 18. Detection probe; 19. Second side plate; 20. Second electric actuator; 21. Light source; 22. Anti-slip groove; 23. Temperature sensor; 24. Heating wire; 25. First fan; 26. Dustproof plate; 27. Mounting frame; 28. Second fan; 29. ​​Transparent window; 30. Controller; 31. First magnetic block; 32. Handle; 33. Second magnetic block. Detailed Implementation

[0026] The present invention will be further described below with reference to the accompanying drawings and specific embodiments, so that those skilled in the art can better understand and implement the present invention. However, the embodiments are not intended to limit the present invention.

[0027] Reference Figures 1-5 As shown, a fluorescent dye molecule detection device of this utility model includes a workbench 1, a detection box 2 fixedly connected to the top of the workbench 1, a light source 21 and a detection probe 18 disposed inside the top of the detection box 2, and a receiving plate 6 disposed inside the bottom of the detection box 2; a placement groove 7 is embedded in the top of the receiving plate 6; a mounting frame 9 is fixedly connected to the bottom of the receiving plate 6, a first motor 8 is fixedly connected to the top of the mounting frame 9, the output shaft of the first motor 8 is fixedly connected to the bottom of the placement groove 7, and the output shaft of the first motor 8 is rotatably connected to the receiving plate 6 through a bearing; sliding rods 5 are fixedly connected to both ends of the receiving plate 6, and slide rails 4 are symmetrically fixed to the inner walls on both sides of the detection box 2, and the sliding rods 5 are slidably connected within the slide rails 4; a plurality of anti-slip grooves 22 are opened at one end of the receiving plate 6.

[0028] During operation, fluorescent dyes include both liquid and solid forms. If the dye is liquid, it can flow freely to ensure even distribution. If the dye is solid, uneven stacking may prevent some fluorescent dye molecules from being effectively excited, leading to deviations in fluorescence detection. The fluorescent dye is poured into the placement tank 7, and the receiving plate 6 is mounted on the slide rail 4 using the cooperation of the slide rod 5 and the slide rail 4. During detection, the first motor 8 needs to be turned on, which drives the placement tank 7 to rotate. The placement tank 7 then drives the fluorescent dye to rotate. Rotation can redistribute the sample particles, more accurately reflecting the overall fluorescence of the sample. Rotation ensures that fluorescent dye molecules at all levels, from the surface to the interior, can be effectively excited.

[0029] Furthermore, such as Figure 5As shown, a second motor 10 is fixedly connected to the top of the detection box 2. The output shaft of the second motor 10 is rotatably connected to the detection box 2 and is fixedly connected to a rotating frame 11. The second motor 10 is located at one end of the rotating frame 11. A reciprocating lead screw 12 is rotatably connected to the rotating frame 11 via a bearing. A moving block 15 is threaded onto the reciprocating lead screw 12. A second side plate 19 is fixedly connected to one side wall of the moving block 15. A second electric push rod 20 is fixedly connected to the bottom of the second side plate 19. A light source 21 is installed on the output end of the second electric push rod 20. A third motor 14 is provided at one end of the reciprocating lead screw 12. The rotating frame 11 is longer than the radius of the placement slot 7.

[0030] During operation, when testing, the fluorescence needs to be excited by the light source 21. By turning on the third motor 14, the output shaft of the third motor 14 drives the reciprocating screw 12 to rotate. The reciprocating screw 12 drives the moving block 15 to reciprocate. The moving block 15 drives the second side plate 19 to reciprocate. The second side plate 19 drives the second electric push rod 20 and the light source 21 to move. At the same time, the second motor 10 is turned on. Through the cooperation of the second motor 10 and the third motor 14, the light source 21 can rotate above the entire placement tank 7, so that there are no dead corners in the fluorescent dye in the placement tank 7 illuminated by the light source 21. By setting the second electric push rod 20, the height of the light source 21 can be easily adjusted, so that the light source 21 can be used to irradiate fluorescent dyes of different thicknesses. The rotating frame 11 rotates in the opposite direction to the placement tank 7.

[0031] Furthermore, such as Figure 5 As shown, a first side plate 16 is fixedly connected to the other side wall of the movable block 15, a first electric actuator 17 is fixedly connected to the bottom of the first side plate 16, and a detection probe 18 is installed on the output end of the first electric actuator 17.

[0032] During operation, by setting the detection probe 18, the fluorescent dye after being irradiated by the light source 21 is activated to start detection. The excitation light source 21 emits excitation light according to the set parameters, which is transmitted through the optical path to irradiate the fluorescent dye. The fluorescence signal generated by the fluorescent dye is received by the detection probe 18 and converted into an electrical signal. The data is transmitted to the data processing system for data analysis.

[0033] Furthermore, such as Figure 3 As shown, a temperature sensor 23 is installed on the top inner wall of the detection box 2, and a dustproof plate 26 is installed on the detection box 2 through a hole; a first fan 25 is installed on the inner wall of the dustproof plate 26; an electric heating wire 24 is provided on the inner side of the first fan 25, and the electric heating wire 24 is electrically connected to the temperature sensor 23.

[0034] During operation, different fluorescent dyes are suitable for different ambient temperatures. The temperature inside the detection chamber 2 is monitored by the temperature sensor 23. Then, the first fan 25 and the heating wire 24 are powered on. The heating wire 24 generates heat, which is blown into the detection chamber 2 by the first fan 25 to keep the fluorescent dye at a suitable temperature for excitation.

[0035] Furthermore, such as Figure 1 As shown, a mounting frame 27 is fixed to the top of the detection box 2, and a second fan 28 is installed inside the mounting frame 27. The second fan 28 is electrically connected to the temperature sensor 23.

[0036] During operation, after the test is completed, the receiving plate 6 needs to be removed, the temperature inside the test chamber 2 is monitored by the temperature sensor 23, and then the second fan 28 is controlled to exhaust the heat to prevent high temperature injury to the operator.

[0037] Furthermore, such as Figure 1 As shown, a sealing plate 3 is slidably connected to the side wall of the detection box 2 away from the first fan 25, and a handle 32 is fixedly connected to the outer wall of the sealing plate 3; a first magnetic block 31 is fixedly connected to the side wall of the detection box 2, and the first magnetic block 31 is in contact with the top of the handle 32; a second magnetic block 33 is embedded and fixedly connected to the top of the workbench 1, and the second magnetic block 33 is in contact with the bottom of the handle 32.

[0038] During operation, the sealing plate 3 is used to seal the detection box 2. During the placement of the receiving plate 6, the handle 32 needs to be lifted upwards to engage with the first magnetic block 31. After the receiving plate 6 is placed, the handle 32 needs to be pulled downwards to engage with the second magnetic block 33 to maintain the sealing of the detection box 2.

[0039] Furthermore, such as Figure 1 As shown, a controller 30 and a transparent window 29 are installed on the side wall of the detection box 2, and the controller 30 is electrically connected to the detection probe 18.

[0040] During operation, the transparent window 29 facilitates observation of the state inside the detection box 2; the controller 30, which is electrically connected to the detection probe 18, facilitates receiving the detection data from the detection probe 18 and performing data processing.

[0041] Furthermore, such as Figure 5 As shown, two guide rods 13 are fixedly connected to the rotating frame 11, and the moving block 15 is slidably connected to the two guide rods 13.

[0042] During operation, the movable block 15 slides on the guide rod 13, which limits the movement of the movable block 15 in the horizontal direction.

[0043] Working principle: Fluorescent dye is poured into the placement tank 7. The receiving plate 6 is installed on the slide rail 4 through the cooperation of the slide rod 5 and the slide rail 4. During detection, the first motor 8 is turned on, which drives the placement tank 7 to rotate. The placement tank 7 drives the fluorescent dye to rotate. The rotation can redistribute the sample particles, more accurately reflecting the overall fluorescence of the sample. The rotation ensures that fluorescent pigment molecules at all levels from the surface to the interior can be effectively excited. During detection, the fluorescence is excited by the light source 21. The third motor 14 is turned on, and the output shaft of the third motor 14 drives the reciprocating screw 12 to rotate. The reciprocating screw 12 drives the moving block 15 to reciprocate. The moving block 15 drives the second side plate 19 to reciprocate. The second side plate 19 drives the second electric push rod 20 and the light source 21 to reciprocate. The light source 21 moves, and simultaneously the second motor 10 is activated. Through the cooperation of the second motor 10 and the third motor 14, the light source 21 can rotate above the entire placement tank 7, ensuring that there are no blind spots in the fluorescent dye within the placement tank 7 where the light source 21 will irradiate. By setting a second electric push rod 20, the height of the light source 21 can be easily adjusted, making the light source 21 suitable for irradiating fluorescent dyes of different thicknesses. The rotating frame 11 rotates in the opposite direction to the placement tank 7. By setting a detection probe 18, after the fluorescent dye is irradiated by the light source 21, the detection probe 18 is activated to start detection. The excitation light source 21 emits excitation light according to the set parameters, which is transmitted through the optical path and irradiates the fluorescent dye. The fluorescence signal generated by the fluorescent dye is received by the detection probe 18 and converted into an electrical signal. The data is transmitted to the data processing system for data analysis.

[0044] Obviously, the above embodiments are merely illustrative examples for clear explanation and are not intended to limit the implementation. Those skilled in the art will recognize that other variations or modifications can be made based on the above description. It is neither necessary nor possible to exhaustively list all possible implementations here. However, obvious variations or modifications derived therefrom are still within the protection scope of this invention.

Claims

1. A fluorescent dye molecule detection device, comprising a workbench (1), a detection box (2) is fixed on the top of the workbench (1), a light source (21) and a detection probe (18) are arranged inside the top end of the detection box (2), and a receiving plate (6) is arranged inside the bottom end of the detection box (2); characterized in that: The top of the bearing plate (6) is embedded with a placing groove (7); the bottom of the bearing plate (6) is fixedly connected with a mounting frame (9), the top of the mounting frame (9) is fixedly connected with a first motor (8), the output shaft of the first motor (8) is fixedly connected at the bottom of the placing groove (7), and the output shaft of the first motor (8) is rotatably connected to the bearing plate (6) through a bearing; both ends of the bearing plate (6) are fixedly connected with sliding rods (5), the inner walls on both sides of the detection box (2) are fixedly connected with sliding rails (4) symmetrically, and the sliding rods (5) are slidably connected in the sliding rails (4) respectively; a plurality of anti-skid grooves (22) are formed in one end of the bearing plate (6).

2. The apparatus of claim 1, wherein: The top of the detection box (2) is fixedly connected with a second motor (10), the output shaft of the second motor (10) is rotatably connected to the detection box (2), and a rotating frame (11) is fixedly connected, and the second motor (10) is arranged at one end of the rotating frame (11); a reciprocating screw rod (12) is rotatably connected to the rotating frame (11) through a bearing, a moving block (15) is threadedly connected to the reciprocating screw rod (12), a second side plate (19) is fixedly connected to one side wall of the moving block (15), a second electric push rod (20) is fixedly connected to the bottom of the second side plate (19), and a light source (21) is mounted on the output end of the second electric push rod (20); one end of the reciprocating screw rod (12) is provided with a third motor (14); the rotating frame (11) is longer than the radius of the placing groove (7).

3. The apparatus of claim 2, wherein: The other side wall of the moving block (15) is fixedly connected with a first side plate (16), the bottom of the first side plate (16) is fixedly connected with a first electric push rod (17), and a detection probe (18) is mounted on the output end of the first electric push rod (17).

4. The apparatus of claim 3, wherein: A temperature sensor (23) is mounted on the top inner wall of the detection box (2), and a dustproof plate (26) is mounted in a hollow manner on the detection box (2); a first fan (25) is mounted on the inner wall of the dustproof plate (26); an electric heating wire (24) is arranged on the inner side of the first fan (25), and the electric heating wire (24) is electrically connected with the temperature sensor (23).

5. The apparatus of claim 4, wherein: A mounting frame (27) is fixedly connected to the top of the detection box (2), a second fan (28) is mounted in the mounting frame (27), and the second fan (28) is electrically connected with the temperature sensor (23).

6. The apparatus of claim 5, wherein: A sealing plate (3) is slidably connected to the side wall of the detection box (2) away from the first fan (25), a handle (32) is fixedly connected to the outer wall of the sealing plate (3); a first magnetic block (31) is fixedly connected to the side wall of the detection box (2), and the top of the handle (32) is attached to the first magnetic block (31); a second magnetic block (33) is fixedly connected to the top of the workbench (1), and the bottom of the handle (32) is attached to the second magnetic block (33).

7. The apparatus of claim 6, wherein: A controller (30) and a transparent window (29) are mounted on the side wall of the detection box (2), and the controller (30) is electrically connected with the detection probe (18).

8. The apparatus of claim 7, wherein: Two guide rods (13) are fixedly connected to the rotating frame (11), and the moving block (15) is slidably connected to the two guide rods (13).

Images