Hydrostatic pressure device for inducing fish polyploidy by combined method

Abstract

The utility model discloses a hydrostatic pressure device for inducing fish polyploidy by a combined method, which comprises a cylinder body, a hydrostatic pressure cylinder barrel is arranged in the cylinder body, a hydraulic cylinder cover is arranged above the hydrostatic pressure cylinder barrel, sealing gaskets are respectively arranged among the cylinder body, the hydrostatic pressure cylinder barrel and the hydraulic cylinder cover, and a hydrostatic pressure cylinder barrel pipeline is connected with a water replenishing tank. A booster pump, a pressure controller and a temperature controller are arranged on the pipeline, the booster pump is in communication connection with the pressure controller, a heating and refrigerating pipe is arranged on the outer surface of the cylinder body and connected with a compressor through a refrigerant conveying pipe, and the compressor is in communication connection with the temperature controller. The hydrostatic pressure device for inducing the fish polyploidy by the combined method can simultaneously realize hydrostatic pressure and heat shock environments, performs tetraploid induction treatment on Yellow River carp fertilized eggs, is convenient for field operation, is safe, reliable, simple and feasible, and has important application value in triploid induction and large-scale culture of cyprinid fishes.

Description

Technical Field

[0001] This utility model belongs to the field of fish polyploid breeding technology, specifically relating to a hydrostatic pressure device for inducing polyploidy in fish using a combined method. Background Technology

[0002] Currently, artificially induced tetraploid fish are mainly induced in many laboratories both domestically and internationally using methods such as cytochalasin B, heat shock, cold shock, and hydrostatic pressure treatment. Many researchers are attempting to produce triploid offspring for breeding by mating fertile tetraploid males with normal diploid females. Fish chromosome manipulation is one of the most active areas of fish genetic breeding research in recent years. To date, triploids have been obtained in nearly 20 farmed fish species using methods such as hydrostatic pressure stress, heat shock, and cold shock. Homotetraploids or allotetraploids have been obtained in *Cyprinus rubrotinctum*, *Carassius amurensis*, *Carassius amurensis*, Nile tilapia, and Mozambique tilapia. Homotetraploids have been reported in grass carp, loach, and *Carassius spp.*, but the success rate of polyploidization is low. After reviewing a large number of domestic and foreign literature, there are currently no reports on inducing tetraploid Yellow River carp using the method of simultaneous heat shock and hydrostatic pressure stress, and there are no reports on corresponding devices for treating fertilized eggs with simultaneous temperature shock and hydrostatic pressure stress. Summary of the Invention

[0003] This invention provides a hydrostatic pressure device for inducing polyploidy in fish using a combined method.

[0004] The purpose of this utility model is achieved in the following manner: a hydrostatic pressure device for inducing polyploidy in fish using a combined method, comprising a cylinder body, a hydrostatic pressure cylinder barrel disposed inside the cylinder body, a cylinder cover disposed above the hydrostatic pressure cylinder barrel, sealing gaskets disposed between the cylinder body, the hydrostatic pressure cylinder barrel and the cylinder cover, the hydrostatic pressure cylinder barrel being connected to a water supply tank via an inlet pipe and an outlet pipe, a booster pump, a pressure controller and a temperature controller being disposed on the inlet pipe or the outlet pipe, the booster pump being communicatively connected to the pressure controller, a heating and cooling pipe being disposed on the outer surface of the cylinder body, the heating and cooling pipe being connected to a compressor via a refrigerant delivery pipe, and the compressor being communicatively connected to the temperature controller.

[0005] Both the cylinder block and cylinder head are made of stainless steel.

[0006] The cylinder head is stepped.

[0007] The sealing gasket is a silicone sealing gasket.

[0008] An insulation layer is also provided on the outer surface of the cylinder.

[0009] Compared with existing technologies, the hydrostatic pressure device for inducing polyploidy in fish disclosed in this utility model can simultaneously achieve hydrostatic pressure and heat shock environment to induce tetraploidy in fertilized eggs of Yellow River carp. This device is easy to operate on-site, safe and reliable, and simple to implement, and has important application value in triploidy induction and large-scale farming of carp. Attached Figure Description

[0010] Figure 1 This is a schematic diagram of the structure of this utility model.

[0011] In the diagram: 1. Cylinder body; 2. Hydrostatic cylinder barrel; 3. Cylinder head; 4. Booster pump; 5. Water tank; 6. Pressure controller; 7. Sealing gasket; 8. Heating and cooling pipe; 9. Compressor; 10. Mounting plate; 11. Refrigerant delivery pipe; 12. Thermostat; 13. Hydraulic device. Detailed Implementation

[0012] The present invention will be further described below with reference to the accompanying drawings and specific embodiments. It should be understood that these embodiments are only for illustrating the present invention and are not intended to limit the scope of the present invention. After reading the contents of the present invention, those skilled in the art can make various modifications or alterations to the present invention, and these equivalent forms also fall within the scope defined by the present invention.

[0013] like Figure 1 As shown, a hydrostatic pressure device for inducing polyploidy in fish using a combined method includes a cylinder 1, a hydrostatic pressure cylinder 2 inside the cylinder 1, a cylinder cover 3 above the hydrostatic pressure cylinder 2, sealing gaskets 7 between the cylinder 1, the hydrostatic pressure cylinder 2, and the cylinder cover 3, the hydrostatic pressure cylinder 2 being connected to a water supply tank 5 via an inlet pipe and an outlet pipe, a booster pump 4, a pressure controller 6, and a thermostat 12 being installed on the inlet or outlet pipe, the booster pump 4 being communicatively connected to the pressure controller 6, a heating and cooling pipe 8 being installed on the outer surface of the cylinder 1, the heating and cooling pipe 8 being connected to a compressor 9 via a refrigerant delivery pipe 11, and the compressor 9 being communicatively connected to the thermostat 12.

[0014] Furthermore, a mounting plate 10 is provided above the cylinder body 1. The mounting plate 10 is fixed to the ground or to the cylinder body by a bracket (not shown in the figure). A hydraulic device 13 is installed on the mounting plate 10. The hydraulic device 13 drives the cylinder head 3 to rise or fall, covering the cylinder head 3 on the hydrostatic cylinder 2 of the cylinder body 1, or pulling it out from the hydrostatic cylinder 2 of the cylinder body 1.

[0015] The heating and cooling pipe 8 can be a single pipe spirally wound around the surface of the cylinder 1, or it can be a series of interconnected pipes.

[0016] Water from the water supply tank 5 is pumped into the hydrostatic cylinder 2 by the booster pump 4. The water in the water supply tank 5 and the hydrostatic cylinder 2 circulates between the inlet and outlet pipes. The pressure in the hydrostatic cylinder 2 is controlled by the pressure controller 6 and regulated by the booster pump 4. The temperature in the hydrostatic cylinder 2 is controlled by the thermostat 12 and regulated by the compressor 9. The thermostat 12 measures the water temperature in the inlet or outlet pipe. When the temperature rises above the preset upper limit, the thermostat 12 sends a cooling command to the compressor 9 to cool the water. When the water temperature drops to the preset lower limit, the thermostat 12 sends a heating command to the compressor 9 to heat the water, maintaining the water temperature in the water supply tank 5 and the hydrostatic cylinder 2 within a certain range.

[0017] Furthermore, the cylinder body 1 is a single piece of stainless steel, either in a block or column shape. A space called the hydrostatic cylinder barrel 2 is machined inside the cylinder body 1. The upper part of the hydrostatic cylinder barrel 2 is stepped, wider at the top and narrower at the bottom, while the lower part is cylindrical. The cylinder head 3 is also made of stainless steel, and its shape matches the stepped shape of the upper part of the hydrostatic cylinder barrel 2. Because a high pressure needs to be maintained inside the hydrostatic cylinder barrel 2, the cylinder head 3 needs to have excellent sealing performance with the cylinder body 1 and the hydrostatic cylinder barrel 2. In addition to high machining precision, silicone sealing gaskets are placed at the two steps of the cylinder head 3. To ensure a good seal, the sealing gaskets can be made thicker, or other sealing gaskets with good sealing performance can be selected.

[0018] Furthermore, an insulation layer is provided on the outer surface of the cylinder 1 to prevent heat loss from the heating and cooling pipes 8.

[0019] The working method of this device is as follows: Fill the hydrostatic cylinder 2 and the water supply tank 5 with water. Turn on the compressor 9 through the thermostat 12 to adjust the water temperature in the hydrostatic cylinder 2 and the water supply tank 5 to the set temperature value. Place the fertilized eggs into the hydrostatic cylinder 2, start the hydraulic cover 3 to seal the hydrostatic cylinder 2, turn on the booster pump 4 to adjust the pressure in the hydrostatic cylinder 2 and the water supply tank 5 to the set value, and monitor and maintain the set temperature and pressure through the pressure controller 6. Induce the fertilized eggs in the hydrostatic cylinder 2 for a certain period of time to induce polyploids.

[0020] Example: Mature Yellow River carp were selectively fertilized. Mature eggs and semen were collected separately. The eggs were placed in a dry container, and semen was slowly added at a volume ratio of 1000 μL:100 mL. Dry fertilization was performed by gently stirring the eggs with a feather to mix them thoroughly. The mixture was then spread on a sieve and immersed in water at 24-26℃ to complete fertilization. The fertilized eggs were then gently shaken to complete the fertilization process. After fertilization, the fertilized eggs continued to develop in the water for 2-4 minutes. Then, the fertilized eggs were placed in a hydrostatic chamber for hydrostatic treatment at a pressure of 550-630 kg / cm². 2The water temperature in the static pressure chamber is maintained at 40-43℃, and the treatment time is 3-5 minutes. After treatment, the fertilized eggs are transferred to a water tank with oxygenation equipment and water flow at 24-26℃ for incubation. After hatching, the fry are transferred to a special breeding pond after they swim horizontally. The DNA count is tested after the fry grow to 3-5cm.

[0021] This invention discloses a hydrostatic pressure device for inducing polyploidy in fish using a combined method. This device can simultaneously achieve hydrostatic pressure and heat shock environment to induce tetraploidy in fertilized eggs of Yellow River carp. The device is easy to operate on-site, safe, reliable, and simple to implement, and has important application value in triploid induction and large-scale farming of carp.

[0022] The above description is only a preferred embodiment of the present utility model. It should be noted that those skilled in the art can make several changes and improvements without departing from the overall concept of the present utility model, and these should also be considered within the protection scope of the present utility model.

Claims

1. A hydrostatic pressure device for inducing polyploidy in fish using a combined method, characterized in that: Includes a cylinder body (1), a hydrostatic cylinder (2) is installed inside the cylinder body (1), a hydraulic cylinder cover (3) is installed above the hydrostatic cylinder (2), a sealing gasket (7) is installed between the cylinder body (1), the hydrostatic cylinder (2) and the hydraulic cylinder cover (3), the hydrostatic cylinder (2) is connected to a water supply tank (5) through an inlet pipe and an outlet pipe, a booster pump (4), a pressure controller (6) and a thermostat (12) are installed on the inlet pipe or the outlet pipe, the booster pump (4) is connected to the pressure controller (6) through communication, a heating and cooling pipe (8) is installed on the outer surface of the cylinder body (1), the heating and cooling pipe (8) is connected to a compressor (9) through a refrigerant delivery pipe (11), and the compressor (9) is connected to the thermostat (12) through communication.

2. The hydrostatic pressure device for inducing polyploidy in fish according to claim 1, characterized in that: Both the cylinder body (1) and the hydraulic cylinder head (3) are made of stainless steel.

3. The hydrostatic pressure device for inducing polyploidy in fish according to claim 1, characterized in that: The hydraulic cylinder head (3) is stepped.

4. The hydrostatic pressure device for inducing polyploidy in fish according to claim 1, characterized in that: The sealing gasket (7) is a silicone sealing gasket.

5. The hydrostatic pressure device for inducing polyploidy in fish according to claim 1, characterized in that: The outer surface of the cylinder (1) is also provided with a heat insulation layer.

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