Circulating tumor cell in-vitro culture fluid filtering device

Abstract

The utility model discloses a kind of circulating tumor cell in vitro culture fluid filtering devices, including shell, the bottom surface of the shell is fixedly installed with rotating motor, the inner bottom wall of the shell is inlayed with first bearing, the inner ring of the first bearing is fixedly connected with rotating rod, the output end of the rotating motor is connected with the bottom end of rotating rod by first bearing, the upper surface of the rotating rod is fixedly connected with clamping block. The cooperation of the device through clamping groove and clamping block, realize rotating motor and filter frame flexible separation, by inserting the insert block of cover plate bottom into storage groove, and using positioning assembly to position cover plate, make synchronous assembly extrude filter frame, the phenomenon of displacement occurs when filter frame rotates, realize that filter frame is conveniently disassembled and installed, to facilitate subsequent maintenance and cleaning, to solve the disassembly of the filter screen of existing device is more cumbersome, leading to the problem of lower work efficiency.

Description

Technical Field

[0001] This utility model relates to the field of cell culture technology, and in particular to a filtration device for circulating tumor cell in vitro culture medium. Background Technology

[0002] Circulating tumor cells (CTCs) refer to tumor cells that detach from the primary tumor or metastatic lesions and enter the peripheral blood circulation. They are one of the core detection targets of "liquid biopsy" and are of great significance for early diagnosis, metastasis monitoring, efficacy evaluation and personalized treatment of tumors. Culture medium is a special culture medium used to maintain and support the survival, growth and function of circulating tumor cells in vitro.

[0003] To ensure the purity of the culture environment and cell viability, circulating tumor cell in vitro culture medium filtration devices are usually used to filter the culture medium. However, after prolonged use, the filter screen of existing circulating tumor cell in vitro culture medium filtration devices gradually becomes clogged with impurities, and disassembling the filter screen is cumbersome, resulting in low work efficiency. Therefore, we propose a circulating tumor cell in vitro culture medium filtration device to solve the above problems. Utility Model Content

[0004] The purpose of this invention is to provide a filtration device for circulating tumor cell in vitro culture medium to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, this utility model provides the following technical solution: A circulating tumor cell in vitro culture medium filtration device includes a housing, a rotary motor fixedly mounted on the bottom surface of the housing, a first bearing embedded in the inner bottom wall of the housing, a rotating rod fixedly connected to the inner ring of the first bearing, the output end of the rotary motor connected to the bottom end of the rotating rod through the first bearing, a locking block fixedly connected to the upper surface of the rotating rod, a filter frame provided inside the housing, a slot formed on the bottom surface of the filter frame, the locking block located inside the slot, symmetrical receiving slots formed on the upper surface of the housing, inserts slidably connected inside the two receiving slots, a cover plate fixedly connected to the upper surface of the two inserts, a second bearing embedded in the bottom surface of the cover plate, a synchronization component fixedly connected to the inner ring of the second bearing, a fixing component provided inside the two inserts, a support plate fixedly connected to the inner wall of the housing, a rotating plate slidably connected to the upper surface of the support plate, and the inner wall of the rotating plate connected to the outer surface of the filter frame.

[0006] In a further embodiment, the bottom surface of the outer shell is fixedly connected to symmetrical support legs, and the bottom surfaces of both support legs are fixedly connected to elastic seats.

[0007] In a further embodiment, a drain pipe is fixedly connected to the outer surface of the housing, and a valve is fixedly installed on the outer surface of the drain pipe.

[0008] In a further embodiment, both of the fixing components include fastening bolts, and each of the two inserts has a threaded hole on its opposite side, with the interior of each threaded hole threadedly connected to the outer surface of the fastening bolt.

[0009] In a further embodiment, the outer surface of the housing has symmetrical openings, and the two fastening bolts, with their opposite sides passing through the openings, extend to the outside of the housing.

[0010] In a further embodiment, the synchronization component includes a turntable with symmetrical positioning holes on its bottom surface. Positioning posts are slidably connected inside each of the two positioning holes, and the bottom ends of both positioning posts are connected to the upper surface of the filter frame. In a further embodiment, a symmetrical spring is fixedly connected inside the slot, and the bottom ends of the two springs are connected to a movable plate, the bottom surface of which contacts the upper surface of the card block.

[0011] Compared with the prior art, the beneficial effects of this utility model are: This device uses a combination of slots and blocks to allow for flexible separation of the rotary motor and the filter frame. By inserting the insert block at the bottom of the cover plate into the storage slot and using the positioning component to position the cover plate, the synchronous component presses the filter frame to position it, preventing displacement during the rotation of the filter frame. This facilitates the disassembly and installation of the filter frame, making subsequent maintenance and cleaning easier. This solves the problem of cumbersome filter screen disassembly and low work efficiency in existing devices. Attached Figure Description

[0012] Figure 1 This is a front view schematic diagram of a circulating tumor cell in vitro culture medium filtration device.

[0013] Figure 2 This is a side view of a circulating tumor cell in vitro culture medium filtration device.

[0014] Figure 3 This is a cross-sectional schematic diagram of a circulating tumor cell in vitro culture medium filtration device.

[0015] Figure 4 Filtering device for circulating tumor cell in vitro culture medium Figure 3 A magnified structural diagram of part A in the middle.

[0016] Figure 5 This is a top view of the outer shell of a circulating tumor cell in vitro culture medium filtration device.

[0017] In the diagram: 1. Outer shell; 2. Cover plate; 3. Support leg; 4. Elastic seat; 5. Movable opening; 6. Fixing component; 601. Fastening bolt; 602. Screw hole; 7. Drain pipe; 8. Valve; 9. Rotary motor; 10. First bearing; 11. Rotating rod; 12. Locking block; 13. Locking slot; 14. Filter frame; 15. Synchronization component; 151. Turntable; 152. Positioning hole; 153. Positioning column; 16. Second bearing; 17. Spring; 18. Movable plate; 19. Storage slot; 20. Insert block; 21. Rotating plate; 22. Bearing plate. Detailed Implementation

[0018] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0019] Please see Figure 1-5 In this utility model, a circulating tumor cell in vitro culture medium filtration device includes a shell 1. A rotary motor 9 is fixedly installed on the bottom surface of the shell 1. A first bearing 10 is embedded in the inner bottom wall of the shell 1. A rotating rod 11 is fixedly connected to the inner ring of the first bearing 10. The output end of the rotary motor 9 is connected to the bottom end of the rotating rod 11 through the first bearing 10. A locking block 12 is fixedly connected to the upper surface of the rotating rod 11. A filter frame 14 is provided inside the shell 1. A slot 13 is opened on the bottom surface of the filter frame 14. The locking block 12 is located inside the slot 13. Symmetrical storage slots 19 are opened on the upper surface of the shell 1. Insert blocks 20 are slidably connected inside the two storage slots 19. A cover plate 2 is fixedly connected to the upper surface of the two insert blocks 20. A second bearing 16 is embedded in the bottom surface of the cover plate 2. A synchronization component 15 is fixedly connected to the inner ring of the second bearing 16. Both inserts 20 have a fixing component 6 inside. The inner wall of the outer shell 1 is fixedly connected to a support plate 22. The upper surface of the support plate 22 is slidably connected to a rotating plate 21. The inner wall of the rotating plate 21 is connected to the outer surface of the filter frame 14. Through the cooperation of the locking block 12 and the locking groove 13, the filter frame 14 can be flexibly separated from the rotary motor 9. The rotary motor 9 drives the filter frame 14 to rotate. Centrifugal force is used to separate the culture medium and impurities in the filter frame 14, so that the filtered culture medium flows into the interior of the outer shell 1, and the impurities flow into the filter frame 14. Through the cooperation of the receiving groove 19, the inserts 20 and the fixing component 6, the cover plate 2 can be fixed to the upper part of the outer shell 1, and the synchronization component 15 can limit the filter frame 14. Through the cooperation of the rotating plate 21 and the support plate 22, the installation height of the filter frame 14 can be ensured.

[0020] The bottom surface of the outer casing 1 is fixedly connected to symmetrical support legs 3, and the bottom surface of each support leg 3 is fixedly connected to an elastic seat 4. Through the cooperation of the support legs 3 and the elastic seat 4, the vibration during the operation of the device can be reduced, ensuring the stability of the device.

[0021] A drain pipe 7 is fixedly connected to the outer surface of the outer shell 1, and a valve 8 is fixedly installed on the outer surface of the drain pipe 7. Through the cooperation of the drain pipe 7 and the valve 8, the culture medium filtered inside the outer shell 1 can be discharged to the outside, which improves the practicality of the device.

[0022] Both fixing components 6 include fastening bolts 601, and each of the two insert blocks 20 has a screw hole 602 on the side that is far apart from each other. The interior of the two screw holes 602 is threaded to the outer surface of the fastening bolt 601. By inserting the fastening bolt 601 through the movable port 5 into the screw hole 602, the insert block 20 can be fixed.

[0023] The outer surface of the housing 1 has symmetrical movable openings 5. The two fastening bolts 601 have their opposite sides passing through the movable openings 5 ​​and extending to the outside of the housing 1. By providing movable openings 5, space can be reserved for the fastening bolts 601 to facilitate installation.

[0024] The synchronization component 15 includes a turntable 151. The bottom surface of the turntable 151 has symmetrical positioning holes 152. Positioning posts 153 are slidably connected inside the two positioning holes 152. The bottom ends of the two positioning posts 153 are connected to the upper surface of the filter frame 14. As the turntable 151 moves with the cover plate 2, it contacts the top of the filter frame 14 and limits the position of the filter frame 14. The positioning posts 153 are inserted into the positioning holes 152, which facilitates the filter frame 14 to drive the turntable 151 to move.

[0025] The slot 13 is fixedly connected with symmetrical springs 17. The bottom ends of the two springs 17 are connected to a movable plate 18. The bottom surface of the movable plate 18 contacts the upper surface of the block 12. After the cover plate 2 is separated from the outer shell 1, the springs 17 use their own elasticity to drive the filter frame 14 to rise, so that the top of the filter frame 14 is higher than the outer shell 1, making it easier for the user to take out the filter frame 14.

[0026] The working principle of this utility model is as follows: To install the filter frame 14, first engage the slot 13 at the bottom of the filter frame 14 with the locking block 12 at the top of the rotating rod 11. Then, insert the insert block 20 into the storage slot 19. Secure the cover plate 2 to the upper part of the outer shell 1 by passing the fastening bolt 601 through the movable opening 5 and screwing it into the screw hole 602. At this time, the positioning pin 153 is inserted into the positioning hole 152 to prevent the filter frame 14 from shaking or shifting, thus completing the installation of the filter frame 14.

[0027] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A device for filtering circulating tumor cell in vitro culture medium, characterized in that: The enclosure includes a housing (1), on which a rotary motor (9) is fixedly mounted. A first bearing (10) is embedded in the inner bottom wall of the housing (1). A rotating rod (11) is fixedly connected to the inner ring of the first bearing (10). The output end of the rotary motor (9) is connected to the bottom end of the rotating rod (11) through the first bearing (10). A locking block (12) is fixedly connected to the upper surface of the rotating rod (11). A filter frame (14) is provided inside the housing (1). A slot (13) is opened on the bottom surface of the filter frame (14). The locking block (12) is located inside the slot (13). A groove (13) is opened on the upper surface of the housing (1). The two storage slots (19) are symmetrically arranged, and each of the two storage slots (19) is slidably connected with a plug (20). The upper surfaces of the two plugs (20) are fixedly connected with a cover plate (2). The bottom surface of the cover plate (2) is inlaid with a second bearing (16). The inner ring of the second bearing (16) is fixedly connected with a synchronization component (15). The two plugs (20) are each provided with a fixing component (6). The inner wall of the outer shell (1) is fixedly connected with a support plate (22). The upper surface of the support plate (22) is slidably connected with a rotating plate (21). The inner wall of the rotating plate (21) is connected to the outer surface of the filter frame (14).

2. The circulating tumor cell in vitro culture medium filtration device according to claim 1, characterized in that: The bottom surface of the outer shell (1) is fixedly connected to symmetrical support legs (3), and the bottom surfaces of the two support legs (3) are fixedly connected to elastic seats (4).

3. The circulating tumor cell in vitro culture medium filtration device according to claim 1, characterized in that: The outer surface of the outer shell (1) is fixedly connected to a drain pipe (7), and a valve (8) is fixedly installed on the outer surface of the drain pipe (7).

4. The circulating tumor cell in vitro culture medium filtration device according to claim 1, characterized in that: Both of the fixing components (6) include fastening bolts (601), and each of the two inserts (20) has a screw hole (602) on the side away from each other. The interior of each screw hole (602) is threaded to the outer surface of the fastening bolt (601).

5. The circulating tumor cell in vitro culture medium filtration device according to claim 4, characterized in that: The outer surface of the outer shell (1) is provided with symmetrical movable openings (5), and the two fastening bolts (601) with their opposite sides passing through the movable openings (5) and extending to the outside of the outer shell (1).

6. The circulating tumor cell in vitro culture medium filtration device according to claim 1, characterized in that: The synchronization component (15) includes a turntable (151), and the bottom surface of the turntable (151) is provided with symmetrical positioning holes (152). Positioning posts (153) are slidably connected inside the two positioning holes (152), and the bottom ends of the two positioning posts (153) are connected to the upper surface of the filter frame (14).

7. The circulating tumor cell in vitro culture medium filtration device according to claim 1, characterized in that: The slot (13) is fixedly connected to a symmetrical spring (17), and the bottom ends of the two springs (17) are connected to a movable plate (18). The bottom surface of the movable plate (18) is in contact with the upper surface of the block (12).

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