Cell culture roller bottle infiltrator

Abstract

The utility model discloses a cell culture transfer bottle infiltrator belongs to cell culture technical field, incline adjusting assembly is equipped with clamping installation component in the upper end of incline adjusting assembly, its technical scheme main point is, hydraulic telescopic rod drives moving rod movement in clamping installation component, through the structure such as hinged link, sliding rod, make clamping rod can adjust the interval flexibly, realize accurate clamping fixed to different specifications transfer bottle, and the placement tray also provides stable support for transfer bottle, and incline adjusting assembly utilizes servo motor drive worm, worm wheel, and then drive rotating shaft rotation, through the meshing of driving bevel gear and driven bevel gear, realize the accurate rotation of angle adjusting shaft, and this transmission structure can accurately control the inclination angle of transfer bottle, make liquid even infiltrate cell layer in transfer bottle, avoid the problem of local excessive flushing or infiltration deficiency, guarantee the consistency of cell growth environment, effectively improve cell activity and culture quality.

Description

Technical Field

[0001] This utility model relates to the field of cell culture technology, specifically to a cell culture roller bottle infiltrator. Background Technology

[0002] In the field of cell culture technology, the cell culture roller bottle is a commonly used cell culture container and is widely used in large-scale adherent cell culture. During the cell culture process, the effective infiltration, washing and medium replacement of cells in the roller bottle are crucial to the cell growth status and culture quality.

[0003] Currently, conventional cell culture roller bottle infiltration operations mostly rely on manual tilting of the roller bottle and injection of liquid. This method is not only inefficient and difficult to meet the needs of large-scale culture, but also results in uneven liquid distribution, which can easily lead to excessive flushing or insufficient infiltration of cells in certain areas, affecting cell viability and culture results. Although some automated equipment can tilt the roller bottle, it has deficiencies in the precise clamping of the roller bottle, the accuracy of angle adjustment, and the automated control of liquid infiltration. It cannot be adapted to roller bottles of different sizes, and it is difficult to accurately adjust the infiltration parameters according to cell characteristics, thus limiting the standardization and industrialization of cell culture. Utility Model Content

[0004] This invention addresses the technical problems existing in the prior art by providing a cell culture roller bottle infiltration device.

[0005] The technical solution of this utility model to solve the above-mentioned technical problems is as follows: It includes a tilt adjustment component, the upper end of which is provided with a clamping and mounting component, and the upper end of the clamping and mounting component is provided with a rotating bottle immersion vessel; The tilt adjustment assembly includes a main housing, inside which a servo motor is fixedly connected. A worm gear is fixedly connected to the output end of the servo motor. A rotating shaft is rotatably connected inside the main housing. A worm wheel is fixedly connected to the middle of the rotating shaft, located inside the main housing. When the servo motor inside the main housing starts, the output shaft drives the worm gear to rotate. The worm gear meshes with the worm wheel, transmitting the rotational motion of the servo motor to the rotating shaft. A driving bevel gear is connected to the upper end of the rotating shaft. The driving bevel gear meshes with a driven bevel gear installed inside the housing. When the rotating shaft rotates, the driving bevel gear drives the driven bevel gear, causing the angle adjustment shaft to rotate, thereby achieving precise adjustment of the tilt angle of the rotating bottle to meet different wetting requirements.

[0006] The beneficial effects of this invention are as follows: The hydraulic telescopic rod in the clamping and mounting assembly drives the moving rod to move. Through the structure of hinged rods and sliding rods, the clamping rods can flexibly adjust the spacing to achieve precise clamping and fixing of rotating bottles of different sizes. The placement tray also provides stable support for the rotating bottles. This design not only enhances the equipment's adaptability to different rotating bottles, but also ensures that the rotating bottles are stable and do not shake during the immersion operation, avoiding liquid spillage or cell damage caused by unstable clamping. It meets the equipment compatibility requirements of large-scale cell culture. The tilt adjustment assembly uses a servo motor to drive the worm gear and worm wheel, which in turn drives the rotating shaft to rotate. Through the meshing of the active bevel gear and the driven bevel gear, the precise rotation of the angle adjustment shaft is achieved. This transmission structure can accurately control the tilt angle of the rotating bottle, so that the liquid evenly immerses the cell layer in the rotating bottle, avoiding the problems of excessive rinsing or insufficient immersion in certain areas, ensuring the consistency of the cell growth environment, and effectively improving cell activity and culture quality.

[0007] Furthermore, an installation housing is fixedly connected to the upper end of the main body shell. The installation housing is equipped with a driving bevel gear. The lower end of the driving bevel gear is fixedly connected to the upper end of the rotating shaft. An angle adjustment shaft is rotatably connected inside the installation housing. A driven bevel gear is fixedly connected to the middle part of the angle adjustment shaft. The driven bevel gear meshes with the driving bevel gear.

[0008] Furthermore, a fixed connecting block is fixedly connected to the middle of the angle adjustment shaft, and an upper mounting plate is fixedly connected to the upper end of the fixed connecting block. A pair of symmetrical L-shaped connecting frames are fixedly connected to the middle of the upper mounting plate. The fixed connecting block fixed in the middle of the angle adjustment shaft will rotate with the angle adjustment shaft, and the upper mounting plate and L-shaped connecting frames at the upper end of the fixed connecting block will also rotate accordingly. The L-shaped connecting frames are used to install the clamping and mounting components. Their rotation allows the clamping and mounting components and the rotating bottle to tilt at a precise angle, ensuring the wetting effect of the liquid in the rotating bottle.

[0009] Furthermore, the clamping and mounting assembly includes a limiting mounting plate fixedly connected to the upper end of a pair of L-shaped connecting frames, a hydraulic telescopic rod fixedly connected to the lower end of the limiting mounting plate, a movable rod fixedly connected to the output end of the hydraulic telescopic rod, and multiple fixed mounting plates fixedly connected to the middle part of the movable rod.

[0010] Furthermore, each of the fixed mounting plates has a pair of symmetrically hinged rods hinged to its center. Each pair of hinged rods has a sliding rod hinged to one end. A sliding block is fixedly connected to the lower end of each sliding rod. Multiple sliding blocks are slidably connected to the limiting mounting plate. The limiting mounting plate is mounted on the tilt adjustment assembly via an L-shaped connecting frame. After the hydraulic telescopic rod is activated, its output end pushes the moving rod up and down. The fixed mounting plate on the moving rod drives the hinged rod to move. Since one end of the hinged rod is hinged to the sliding rod and the other end is hinged to the fixed mounting plate, the movement of the moving rod causes the hinged rod to push the sliding rod. The sliding block at the lower end of the sliding rod slides within the strip-shaped sliding hole of the limiting mounting plate, thereby adjusting the spacing of the clamping rod to clamp and fix bottles of different sizes. The placement plate at the upper end of the moving rod provides bottom support for the rotating bottle.

[0011] Furthermore, a clamping rod is fixedly connected to the upper end of each sliding rod, and a placement tray is fixedly connected to the upper end of the moving rod, which facilitates the placement and support of the immersion vessel.

[0012] Furthermore, the upper end of the mounting housing is provided with a strip-shaped through hole that cooperates with the rotation of the fixed connecting block, so that the fixed connecting block can rotate through it.

[0013] Furthermore, the middle part of the limiting mounting plate is provided with multiple strip-shaped sliding holes that cooperate with the sliding block for sliding limitation, thereby playing the role of cooperating with the sliding block for sliding limitation. Attached Figure Description

[0014] Figure 1 This is a schematic diagram of the overall structure of this utility model; Figure 2 This is a schematic diagram showing the overall disassembly of this utility model; Figure 3 This is a cross-sectional view of the main body shell of this utility model; Figure 4 This is a schematic diagram of the disassembled structure of the clamping and mounting assembly of this utility model; Figure 5 This is a schematic diagram of the connection structure between the limiting mounting plate and the sliding block of this utility model; Figure 6 This is a schematic diagram of the overall structure of the sliding rod of this utility model.

[0015] The attached diagram lists the components represented by each number as follows: 1. Tilt adjustment assembly; 2. Clamping and mounting assembly; 3. Rotating bottle immersion vessel; 101. Main body shell; 102. Servo motor; 103. Worm gear; 104. Rotating shaft; 105. Worm wheel; 106. Mounting shell; 107. Driving bevel gear; 108. Angle adjustment shaft; 109. Driven bevel gear; 110. Fixed connecting block; 111. Upper mounting plate; 112. L-shaped connecting frame; 201. Limiting mounting plate; 202. Hydraulic telescopic rod; 203. Moving rod; 204. Fixed mounting plate; 205. Hinge rod; 206. Sliding rod; 207. Sliding block; 208. Clamping rod; 209. Placement tray. Detailed Implementation

[0016] The technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments. Based on the embodiments of this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application.

[0017] In the description of this application, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of the stated features. In the description of this application, "multiple" means two or more, unless otherwise explicitly specified.

[0018] In the description of this application, it should also be noted that, unless otherwise expressly specified and limited, the terms "set up," "install," "connect," and "link" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this technology based on the specific circumstances.

[0019] In the description of this application, spatial relation terms such as "below," "under," "below," "below," "above," "over," etc., are used herein to describe the relationship between one element or feature shown in the figures and other elements or features. It should be understood that, in addition to the orientation shown in the figures, spatial relation terms also include different orientations of the device in use and operation. For example, if the device in the figures is flipped, an element or feature described as "below" or "under" other elements or features would be oriented "over" other elements or features. Therefore, the exemplary terms "below" and "under" can include both upper and lower orientations. Furthermore, the device may also include other orientations (e.g., rotated 90 degrees or other orientations), and the spatial descriptive terms used herein are interpreted accordingly.

[0020] In the description of this application, the term "for example" is used to mean "used as an example, illustration, or description." Any embodiment described as "for example" in this application is not necessarily to be construed as being more preferred or advantageous than other embodiments. The following description is provided to enable any person skilled in the art to implement and use the present invention. Details are set forth in the following description for purposes of explanation. It should be understood that those skilled in the art will recognize that the present invention can be implemented without using these specific details. In other instances, well-known structures and processes will not be described in detail to avoid obscuring the description of the present invention with unnecessary detail. Therefore, the present invention is not intended to be limited to the embodiments shown, but is consistent with the broadest scope of the principles and features disclosed in this application.

[0021] Example 1 Figure 1 This is a structural diagram of a cell culture roller bottle infiltrator provided in an embodiment of the present invention. Figure 2 This is a cross-sectional view of the internal structure of the purification tank of this utility model. Figure 1 , Figure 2 , Figure 3 As shown, the device includes a tilt adjustment component 1, a clamping and mounting component 2 at the upper end of the tilt adjustment component 1, and a rotating bottle immersion vessel 3 at the upper end of the clamping and mounting component 2. The tilt adjustment assembly 1 includes a main housing 101, a servo motor 102 fixedly connected inside the main housing 101, a worm gear 103 fixedly connected to the output end of the servo motor 102, a rotating shaft 104 rotatably connected inside the main housing 101, and a worm wheel 105 fixedly connected to the middle of the rotating shaft 104 and located inside the main housing 101.

[0022] Example 2 Based on Embodiment 1, the present invention can be further improved in the following ways, such as... Figure 4 , Figure 5 , Figure 6 As shown, a mounting housing 106 is fixedly connected to the upper end of the main housing 101. An active bevel gear 107 is provided inside the mounting housing 106. The lower end of the active bevel gear 107 is fixedly connected to the upper end of the rotating shaft 104. An angle adjustment shaft 108 is rotatably connected inside the mounting housing 106. A driven bevel gear 109 is fixedly connected to the middle of the angle adjustment shaft 108. The driven bevel gear 109 meshes with the active bevel gear 107.

[0023] A fixed connecting block 110 is fixedly connected to the middle of the angle adjustment shaft 108. An upper mounting plate 111 is fixedly connected to the upper end of the fixed connecting block 110. A pair of mutually symmetrical L-shaped connecting brackets 112 are fixedly connected to the middle of the upper mounting plate 111.

[0024] The clamping and mounting assembly 2 includes a limiting mounting plate 201 fixedly connected to the upper end of a pair of L-shaped connecting frames 112. A hydraulic telescopic rod 202 is fixedly connected to the lower end of the limiting mounting plate 201. A moving rod 203 is fixedly connected to the output end of the hydraulic telescopic rod 202. Multiple fixed mounting plates 204 are fixedly connected to the middle part of the moving rod 203.

[0025] Each fixed mounting plate 204 has a pair of symmetrical hinge rods 205 hinged to its middle part. Each pair of hinge rods 205 has a sliding rod 206 hinged to one end. The lower end of the sliding rod 206 is fixedly connected to a sliding block 207. Multiple sliding blocks 207 are slidably connected to the limiting mounting plate 201.

[0026] Each sliding rod 206 has a clamping rod 208 fixedly connected to its upper end, and a placement plate 209 is fixedly connected to the upper end of the moving rod 203.

[0027] The upper end of the mounting housing 106 has a strip-shaped through hole that rotates to accommodate the fixed connecting block 110.

[0028] The middle part of the limiting mounting plate 201 has multiple strip-shaped sliding holes for sliding limit with the sliding block 207.

[0029] After the hydraulic telescopic rod 202 is activated, the output end pushes the moving rod 203 to move up and down. The fixed mounting plate 204 in the middle of the moving rod drives the hinge rod 205 to move. One end of the hinge rod is hinged to the sliding rod 206, and the other end is hinged to the fixed mounting plate, so that the sliding block 207 at the lower end of the sliding rod slides laterally in the strip-shaped sliding hole of the limiting mounting plate 201, thereby driving the clamping rod 208 at the upper end of the sliding rod to open and close, adapting to the diameter of different sizes of rotating bottles. The placement plate 209 at the upper end of the moving rod supports the bottom of the rotating bottle, forming a double fixing structure of "clamping + support" to ensure the stability of the rotating bottle. The servo motor 102 drives the worm gear 103 to rotate. Through the meshing of the worm gear and the worm wheel 105, the power is transmitted to the rotating shaft 104. The active bevel gear 107 at the upper end of the rotating shaft rotates accordingly, and drives the driven bevel gear 109 and the angle adjustment shaft 108 in the mounting housing 106 to rotate synchronously. The fixed connecting block 110 in the middle of the angle adjustment shaft is connected to the upper mounting plate 111 and L The shaped connecting bracket 112 drives the clamping and mounting components and the rotating bottle to tilt as a whole. The strip-shaped through hole at the upper end of the mounting shell provides space for the fixed connecting block to rotate, and the strip-shaped sliding hole of the limiting mounting plate ensures that the sliding block moves smoothly.

[0030] While embodiments or examples of this disclosure have been described with reference to the accompanying drawings, it should be understood that the above embodiments are merely exemplary embodiments or examples, and the scope of this utility model is not limited by these embodiments or examples, but only by the granted claims and their equivalents. Various elements in the embodiments or examples may be omitted or replaced by their equivalents. Furthermore, the steps may be performed in a different order than that described in this disclosure. Further, various elements in the embodiments or examples may be combined in various ways. Importantly, as the technology evolves, many elements described herein can be replaced by equivalents that appear after this disclosure.

Claims

1. A cell culture roller bottle infiltrator comprising a tilt adjustment assembly (1), characterized in that: The upper end of the tilt adjustment component (1) is provided with a clamping and mounting component (2), and the upper end of the clamping and mounting component (2) is provided with a rotating bottle immersion vessel (3). The tilt adjustment assembly (1) includes a main housing (101), a servo motor (102) is fixedly connected inside the main housing (101), a worm gear (103) is fixedly connected to the output end of the servo motor (102), a rotating shaft (104) is rotatably connected inside the main housing (101), and a worm wheel (105) is fixedly connected to the middle of the rotating shaft (104) and inside the main housing (101).

2. A cell culture roller bottle infiltrator according to claim 1, wherein: The upper end of the main body shell (101) is fixedly connected to the mounting shell (106). The interior of the mounting shell (106) is provided with a driving bevel gear (107). The lower end of the driving bevel gear (107) is fixedly connected to the upper end of the rotating shaft (104). The interior of the mounting shell (106) is rotatably connected to an angle adjustment shaft (108). The middle part of the angle adjustment shaft (108) is fixedly connected to a driven bevel gear (109). The driven bevel gear (109) meshes with the driving bevel gear (107).

3. A cell culture roller bottle infiltrator according to claim 2, wherein: A fixed connecting block (110) is fixedly connected to the middle of the angle adjustment shaft (108), and an upper mounting plate (111) is fixedly connected to the upper end of the fixed connecting block (110). A pair of mutually symmetrical L-shaped connecting brackets (112) are fixedly connected to the middle of the upper mounting plate (111).

4. The cell culture spinner flask infiltrator of claim 1, wherein: The clamping and mounting assembly (2) includes a limiting mounting plate (201) fixedly connected to the upper end of a pair of L-shaped connecting frames (112). A hydraulic telescopic rod (202) is fixedly connected to the lower end of the limiting mounting plate (201). A moving rod (203) is fixedly connected to the output end of the hydraulic telescopic rod (202). A plurality of fixed mounting plates (204) are fixedly connected to the middle part of the moving rod (203).

5. A cell culture spinner flask infiltrator according to claim 4, wherein: Each of the fixed mounting plates (204) has a pair of symmetrical hinge rods (205) hinged to its middle part. Each pair of hinge rods (205) has a sliding rod (206) hinged to one end. The lower end of the sliding rod (206) is fixedly connected to a sliding block (207). Multiple sliding blocks (207) are slidably connected to the limiting mounting plate (201).

6. A cell culture spinner flask infiltrator according to claim 5, wherein: Each of the sliding rods (206) has a clamping rod (208) fixedly connected to its upper end, and the moving rod (203) has a placement plate (209) fixedly connected to its upper end.

7. The cell culture spinner flask infiltrator of claim 2, wherein: The upper end of the mounting housing (106) is provided with a strip-shaped through hole that is rotatable with the fixed connecting block (110).

8. The cell culture spinner flask infiltrator of claim 4, wherein: The limiting mounting plate (201) has multiple strip-shaped sliding holes in the middle for sliding and limiting the sliding of the sliding block (207).

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